CN105624219A - Method for producing citric acid by fermenting wheat starch milk - Google Patents
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Abstract
The invention discloses a method for producing citric acid by fermenting wheat starch milk, the byproduct of vital gluten industrial production. Wheat starch milk, the byproduct of vital gluten industrial production, is directly used for being mixed with thermostable alpha amylase for high-temperature spraying and liquefying, the mixture enters a fermenting system or is mixed with other starch raw material liquefied liquid to be fermented, and citric acid is extracted through a calcium salt method or a chromatography. No processing of other modes is conducted in the wheat starch milk liquefying process, the technological process is simplified, production cost is saved, and sewage emission is reduced; meanwhile, wheat starch milk is used for replacement or mixed with other starch raw materials for use, and therefore the utilization rate of wheat starch can be increased, and the problem that other starch raw materials are high in cost is solved.
Description
Technical field
The present invention relates to a kind of method of fermentative Production citric acid, particularly relate to a kind of method producing citric acid with wheat starch milk fermentation, wherein wheat starch breast is the by product of industrial production gluten powder.
Background technology
Citric acid is a kind of important organic acid, mainly through fermentative Production, has pleasant tart flavour, safety non-toxic, can directly be absorbed metabolism by organism, and purposes is very extensive. Its foodstuffs industry, medicine, cosmetics, makeup and other industrial be widely used, such as aspects such as the foodstuff productions such as beverage, jam and jelly, candy, bean product and seasonings, metal purification, de-sludging, improvement industrial gaseous waste, waste water.
Citric acid is nearly all utilize carbohydrate for matrix now, produced by the method for fermentation, conventional carbohydrate comes as corn, cassava, preserved fruit, rice, wheat, Chinese sorghum etc., wherein extensive using corn as citric acid fermentation raw material, but corn is as main grain, in short supply, price height. Of common occurrence about taking wheat starch as the report that raw material carries out fermenting. The patent of invention that salt pond, Ningxia in 1993 Citric Acid Plant is announced describes the processing method that the by product wheat starch breast after utilizing wheat-flour to extract gluten powder carries out citric acid fermentation as raw material, its main technique is that the starch slurry obtained by extraction gluten powder is through settling tank supernatant 8h, just carrying out liquefying, ferment, the shortcoming of the method is that fermentation raw material is single; Operation is loaded down with trivial details, the supernatant time is long; Starch slurry is perishable, even if settling tank is adopted sterilising treatment, also cannot prevent because of flour or and the miscellaneous bacteria brought into of face water itself cause the phenomenon of microbiological contamination; After supernatant, the soluble proteins water of the row of taking out adds the burden of sewage disposal; Fermentation and acid rate is low, and average acid production rate only has 11.8%. 1996 so far, and phase sequence discloses taking wheat B starch and wheat flour as raw material, and the patent of fermentation production of citric acid, the deficiency that these patents generally exist is that processing method is loaded down with trivial details, facility investment expense is big, raw material is single, cost height.
In the patent of invention in China's notification number to be CN101407830, publication date be on April 15th, 2009, disclose " being that fermenting raw materials produces citric acid technique taking whole meal flour ", technical scheme is: industry flour is added water and makes face slurry, separation by a., obtains wet gluten and starch breast; B. in gluten, add 1-2 times of water, sieve, washing, obtain wet gluten and rare starch breast; C. wet gluten dried, pulverize, pack, be i.e. obtained commodity gluten powder; The breast separation of rare starch, obtains starch breast; D. starch breast gradation obtained is collected, liquefaction, liquefier pol 15-16%, access bacterial classification, and fermentation, obtains fermented liquid; Concentrated, crystallization, separation, obtain citric acid. But there is technical process complexity, the shortcoming that fermentation level is not high.
Summary of the invention
For overcoming above-mentioned shortcoming, the present invention extracts by product with gluten powder, namely the mixture of wheat A starch and wheat B starch is raw material, directly add high temperature resistant starch to liquefy, without the operation such as supernatant, Plate Filtration, whole production process non-pollutant discharge, through plate-type heat exchanger, starch slurry is heated in advance, reduce the steam consumption of liquefaction process, it is to increase production efficiency, reduce production cost. It is an object of the invention to provide that a kind of technique is simple, production efficiency height, equipment, raw material and the low citric acid production method of cost of supplementary product, comprise the following steps:
Flour mixes with water, slaking, obtains wheat A starch slurry and gluten slurries through cyclone separator, and gluten slurries obtain wheat B starch slurry and wet gluten through gluten sieve, and wheat A starch slurry and wheat B starch slurry are mixed to get wheat starch breast.
Wheat starch breast after pretreatment, or mixes with other liquefying starchiness raw material liquid, is mixed with seed culture medium and fermention medium, aspergillus niger spore access seed culture medium enlarged culturing, moves to, in fermentor tank, obtaining finished lemon/lime acid through fermentation, extraction.
Further, wheat starch breast is the by product after industrial production gluten powder, i.e. the mixed solution of wheat A starch and wheat B starch, and total sugar content is 15-30%, and total nitrogen content is 0.08-0.20%.
Further, the pretreatment process of wheat starch breast is that wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, mix with ��-fire resistant alpha-diastase, ��-fire resistant alpha-diastase amount of adding is 20-60U/g starch, through being steam heated to 100-110 DEG C, insulation 5-15min, is then cooled to 80-95 DEG C, maintains 2-5h.
Further, after pre-treatment, the DE value of wheat starch breast is 25-40%.
Further, other starchy material is one or more in Semen Maydis powder, sorghum flour, Tapioca Starch, starch, sweet potato powder, and pretreated total sugar content is 15-30%.
Further, seed culture medium is by wheat starch breast or presses 1:1-1:9 mixed preparing with other starchy material, and surely hold with clear water or other water source, other water source is the waste water produced in citric acid leaching process, total sugar content is 7-13%, having when needing, additional sulfuric acid ammonium, bean cake powder or cottonseed are as nitrogenous source, and seed culture medium total nitrogen content is 0.15-0.30%.
Preferably, other starchy material first liquefies, and seed culture medium presses 1:1-1:5 mixing by wheat starch breast and other starchiness liquefier, and is settled to, with the relevant water source of clear water or other, the seed culture medium that total sugar content is 7-13%, additional nitrogenous source, the total nitrogen content of adjustment seed culture medium is 0.15-0.30%, under 121 DEG C of conditions, and sterilizing 30min, when being cooled to 37-39 DEG C, by the amount of 2-5*104g/ml access aspergillus niger spore, carry out seed culture, incubation time 20-30h.
Preferably, aspergillus niger spore is using wheat starch breast as fermentation raw material, the adaptation that screening obtains and wheat starch milk fermentation, the aspergillus niger strain that stability is high.
Further, prepare after fermention medium with wheat starch breast or with other starchy material mixing citric acid, saccharifying enzyme need to be added in the fermentation medium, saccharifying enzyme add-on is 80-200U/ml fermention medium, after sterilizing, adds saccharifying enzyme when temperature is down to 65-70 DEG C, fermention medium temperature-fall period carries out saccharification, after saccharification, feed liquid DE value is 60-90%, and fermention medium total sugar content is 15-18%, and total nitrogen content is 0.08-0.12%.
Preferably, fermention medium by wheat starch breast or wheat starch breast with other liquefying starchiness raw material liquid according to 1:1-1:9 mixed preparing, surely holding with clear water or other water source, control fermention medium total reducing sugar is 15-18%, and total nitrogen content is 0.08-0.12%, then at 95 DEG C, sterilizing 30min, when being cooled to 65-70 DEG C, adds saccharifying enzyme and liquefies, the saccharifying enzyme amount of adding is 80-200U/ml fermention medium, saccharification time 1-3h.
Further, by cultivating the seed culture medium of 20-30h, it is forwarded in fermention medium and ferments, then extract citric acid by calcium salt method or chromatography.
The technique effect that the present invention is useful is:
1. the by product producing gluten powder, without any process, directly carries out liquefying and fermenting, and simplifies production technique, shortens Production Flow Chart, it is to increase the utilization ratio of raw material;
2. wheat starch breast is first heated by plate-type heat exchanger, it is achieved that in citric acid production process, the recycling of heat, can reduce the consumption of steam simultaneously, save production cost;
3. wheat starch breast passes through steam rapid heat to 100-110 DEG C, high temperature maintains 5-15min, it is cooled to 80-95 DEG C, maintain 2-5h, starch granules abundant water-swelling, gelatinization after pyroprocessing can be made, enzyme liquid fully being contacted with starch granules, farthest maintains the vigor of alpha-amylase, liquefaction is thoroughly;
4. in fermention medium sterilizing temperature-fall period, completing the saccharification of feed liquid, compared with other patent saccharifying, can save the additional working hour of 8h, it is to increase working efficiency, this technique meets the requirement that enterprise increases work efficiency;
5. seed culture is formulated in proportion with other liquefying starchiness raw material liquid by wheat starch breast, compound material is fermented, ensure that the consistence of seed culture medium and fermention medium, it is to increase the adaptive faculty of aspergillus niger strain, be conducive to improving fermentation efficiency;
6. screening is to wheat starch breast strong adaptability, bacterial classification that stability is high, avoids bacterial classification because being not suitable with by wheat starch breast, and the financial loss caused.
Accompanying drawing explanation
Fig. 1 is the process flow sheet of the present embodiment.
Embodiment
Below in conjunction with accompanying drawing, the better embodiment of the present invention is described in detail, so that advantages and features of the invention can be easier to be readily appreciated by one skilled in the art, thus protection scope of the present invention is made more explicit defining.
Embodiment 1
With a method for wheat starch milk fermentation citric acid, its key step comprises:
(1) water and flour are in the ratio of 0.7:1-1:1 and face, slaking 1h;
(2) through cyclone separator, wheat A starch slurry is isolated;
(3) isolating wheat B starch slurry through gluten sieve, wheat A, B starch slurry concentrates in a container, mixes, and forms wheat starch breast, and wheat starch breast total sugar content is 15-20%, and total nitrogen content is 0.08-0.20%;
(4) wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, by the enzyme concentration of 20-60U/g starch, add fire resistant alpha-diastase, instant steam is utilized to be heated to 100 DEG C, maintain 15min, utilize the System and Surroundings temperature difference so that feed temperature is down to 85-95 DEG C, maintaining 2h, the lo-tionized liquid DE value of gained wheat starch is 25-30%;
(5) in seeding tank, 10-15m is added3The lo-tionized liquid of wheat starch that step (4) obtains, and it is settled to 26m with clear water3, seeding tank total reducing sugar is 7-11%, and adjustment total nitrogen content is 0.15-0.20%, 121 DEG C of sterilizing 30min, when being cooled to 37-39 DEG C, presses the amount access aspergillus niger spore of 2-5*104/ml, carries out aerlbic culture, incubation time 20-30h;
(6) in fermentor tank, 270-310m is added3Through the lo-tionized liquid of wheat starch that step (3) obtains, and it is settled to 295-310m with water3, fermention medium total reducing sugar is 15-17%, lowers the temperature after 95 DEG C of sterilizing 30min, when temperature is down to 65-70 DEG C, adds the saccharifying enzyme of 80-200U/ml fermention medium;
(7) when fermention medium temperature is down to 38-40 DEG C, start to move and plant, carry out aerlbic culture;
(8) fermentation period is 55-70h, and fermentation ends acid production rate is 14.5-16.8%, and fermentation conversion rate is 97-101.23%;
(9) extract citric acid by " calcium salt method " or " chromatography ", obtain citric acid finished product.
Embodiment 2
With a method for wheat starch milk fermentation citric acid, its key step comprises:
(1) water and flour are in the ratio of 0.7:1-1:1 and face, slaking 1h;
(2) through cyclone separator, wheat A starch slurry is isolated;
(3) isolating wheat B starch slurry through gluten sieve, wheat A, B starch slurry concentrates in a container, mixes, and forms wheat starch breast, and wheat starch breast total sugar content is 20-25%, and total nitrogen content is 0.08-0.20%;
(4) wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, by the enzyme concentration of 20-60U/g starch, add fire resistant alpha-diastase, instant steam is utilized to be heated to 105 DEG C, maintain 10min, utilize the System and Surroundings temperature difference so that feed temperature is down to 85-95 DEG C, maintaining 3-4h, the lo-tionized liquid DE value of gained wheat starch is 25-35%;
(5) in seeding tank, 13m is added3The lo-tionized liquid of wheat starch that step (4) obtains, and it is settled to 26m with clear water3, seeding tank total reducing sugar is 10-13%, and adjustment total nitrogen content is 0.15-0.20%, 121 DEG C of sterilizing 30min, when being cooled to 37-39 DEG C, presses the amount access aspergillus niger spore of 2-5*104/ml, carries out aerlbic culture, incubation time 20-30h;
(6) in fermentor tank, 210-280m is added3Through the lo-tionized liquid of wheat starch that step (3) obtains, and it is settled to 295-310m with water3, fermention medium total reducing sugar is 17-18%, lowers the temperature after 95 DEG C of sterilizing 30min, when temperature is down to 65-70 DEG C, adds the saccharifying enzyme of 80-200U/ml fermention medium;
(7) when fermention medium temperature is down to 38-40 DEG C, start to move and plant, carry out aerlbic culture;
(8) fermentation period is 65-80h, and fermentation ends acid production rate is 16.0-17.2%, and fermentation conversion rate is 94-97.7%;
(9) extract citric acid by " calcium salt method " or " chromatography ", obtain citric acid finished product.
Embodiment 3
With a method for wheat starch milk fermentation citric acid, its key step comprises:
(1) water and flour are in the ratio of 0.7:1-1:1 and face, slaking 1h;
(2) through cyclone separator, wheat A starch slurry is isolated;
(3) isolating wheat B starch slurry through gluten sieve, wheat A, B starch slurry concentrates in a container, mixes, and forms wheat starch breast, and wheat starch breast total sugar content is 25-30%, and total nitrogen content is 0.08-0.20%;
(4) wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, by the enzyme concentration of 20-60U/g starch, add fire resistant alpha-diastase, instant steam is utilized to be heated to 110 DEG C, maintain 15min, utilize the System and Surroundings temperature difference so that feed temperature is down to 85-95 DEG C, maintaining 4-5h, the lo-tionized liquid DE value of gained wheat starch is 30-40%;
(5) in seeding tank, 11m is added3The lo-tionized liquid of wheat starch that step (4) obtains, and it is settled to 26m with clear water3, seeding tank total reducing sugar is 12-13%, and adjustment total nitrogen content is 0.15-0.20%, and 121 DEG C of sterilizing 30min, when being cooled to 37-39 DEG C, press 2-5*104The amount access aspergillus niger spore of individual/ml, carries out aerlbic culture, incubation time 20-30h;
(6) in fermentor tank, 180-225m is added3Through the lo-tionized liquid of wheat starch that step (3) obtains, and it is settled to 295-310m with water3, fermention medium total reducing sugar is 17-18%, lowers the temperature after 95 DEG C of sterilizing 30min, when temperature is down to 65-70 DEG C, adds the saccharifying enzyme of 80-200U/ml fermention medium;
(7) when fermention medium temperature is down to 38-40 DEG C, start to move and plant, carry out aerlbic culture;
(8) fermentation period is 65-80h, and fermentation ends acid production rate is 16.3-17.6%, and fermentation conversion rate is 95.0-98.5%;
(9) extract citric acid by " calcium salt method " or " chromatography ", obtain citric acid finished product.
Embodiment 4
With a method for wheat starch milk fermentation citric acid, its key step comprises:
(1) water and flour are in the ratio of 0.7:1-1:1 and face, slaking 1h;
(2) through cyclone separator, wheat A starch slurry is isolated;
(3) isolating wheat B starch slurry through gluten sieve, wheat A, B starch slurry concentrates in a container, mixes, and forms wheat starch breast, and wheat starch breast total sugar content is 15-20%, and total nitrogen content is 0.08-0.20%;
(4) wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, by the enzyme concentration of 20-60U/g starch, add fire resistant alpha-diastase, instant steam is utilized to be heated to 100 DEG C, maintain 15min, utilize the System and Surroundings temperature difference so that feed temperature is down to 85-95 DEG C, maintaining 2h, the lo-tionized liquid DE value of gained wheat starch is 25-30%;
(5) waste water that other starchy material is undertaken liquefying or utilizing citric acid leaching process to produce by patent CN104109697A by existing ripe liquefaction process liquefies, and obtains corresponding liquefier, comprises the clear liquid of liquefaction and the mixed liquid of liquefaction;
(6) adding the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in seeding tank, both ratios are 1:1-1:3, and the waste water produced with clear water or citric acid leaching process is settled to 26m3, seeding tank total reducing sugar is 7-11%, and adjustment total nitrogen content is 0.15-0.20%, and 121 DEG C of sterilizing 30min, when being cooled to 37-39 DEG C, press 2-5*104The amount access aspergillus niger spore of individual/ml, carries out aerlbic culture, incubation time 20-30h;
(7) adding the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in fermentor tank, both ratios are 1:1-1:3, and the waste water produced with clear water or citric acid leaching process is settled to 295-310m3, fermention medium total reducing sugar is 15-17%, lowers the temperature after 95 DEG C of sterilizing 30min, when temperature is down to 65-70 DEG C, adds the saccharifying enzyme of 80-200U/ml fermention medium;
(8) when fermention medium temperature is down to 38-40 DEG C, start to move and plant, carry out aerlbic culture;
(9) fermentation period is 50-60h, and fermentation ends acid production rate is 15.2-17.5%, and fermentation conversion rate is 99.0-103.2%;
(10) extract citric acid by " calcium salt method " or " chromatography ", obtain citric acid finished product.
Embodiment 5
With a method for wheat starch milk fermentation citric acid, its key step comprises:
(1) water and flour are in the ratio of 0.7:1-1:1 and face, slaking 1h;
(2) through cyclone separator, wheat A starch slurry is isolated;
(3) isolating wheat B starch slurry through gluten sieve, wheat A, B starch slurry concentrates in a container, mixes, and forms wheat starch breast, and wheat starch breast total sugar content is 20-25%, and total nitrogen content is 0.08-0.20%;
(4) wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, by the enzyme concentration of 20-60U/g starch, add fire resistant alpha-diastase, instant steam is utilized to be heated to 105 DEG C, maintain 10min, utilize the System and Surroundings temperature difference so that feed temperature is down to 85-95 DEG C, maintaining 3-4h, the lo-tionized liquid DE value of gained wheat starch is 25-35%;
(5) waste water that other starchy material is undertaken liquefying or utilizing citric acid leaching process to produce by patent CN104109697A by existing ripe liquefaction process liquefies, and obtains corresponding liquefier, comprises the clear liquid of liquefaction and the mixed liquid of liquefaction;
(6) adding the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in seeding tank, both ratios are 1:3-1:5, and the waste water produced with clear water or citric acid leaching process is settled to 26m3, seeding tank total reducing sugar is 10-13%, and adjustment total nitrogen content is 0.15-0.20%, and 121 DEG C of sterilizing 30min, when being cooled to 37-39 DEG C, press 2-5*104The amount access aspergillus niger spore of individual/ml, carries out aerlbic culture, incubation time 20-30h;
(7) adding the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in fermentor tank, both ratios are 1:3-1:5, and are settled to 295-310m with water3, fermention medium total reducing sugar is 17-18%, lowers the temperature after 95 DEG C of sterilizing 30min, when temperature is down to 65-70 DEG C, adds the saccharifying enzyme of 80-200U/ml fermention medium;
(8) when fermention medium temperature is down to 38-40 DEG C, start to move and plant, carry out aerlbic culture;
(9) fermentation period is 55-75h, and fermentation ends acid production rate is 16.2-17.6%, and fermentation conversion rate is 96.2-99.6%;
(10) extract citric acid by " calcium salt method " or " chromatography ", obtain citric acid finished product.
Embodiment 6
With a method for wheat starch milk fermentation citric acid, its key step comprises:
(1) water and flour are in the ratio of 0.7:1-1:1 and face, slaking 1h;
(2) through cyclone separator, wheat A starch slurry is isolated;
(3) isolating wheat B starch slurry through gluten sieve, wheat A, B starch slurry concentrates in a container, mixes, and forms wheat starch breast, and wheat starch breast total sugar content is 25-30%, and total nitrogen content is 0.08-0.20%;
(4) wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, by the enzyme concentration of 20-60U/g starch, add fire resistant alpha-diastase, instant steam is utilized to be heated to 110 DEG C, maintain 15min, utilize the System and Surroundings temperature difference so that feed temperature is down to 85-95 DEG C, maintaining 4-5h, the lo-tionized liquid DE value of gained wheat starch is 30-40%;
(5) waste water that other starchy material is undertaken liquefying or utilizing citric acid leaching process to produce by patent CN104109697A by existing ripe liquefaction process liquefies, and obtains corresponding liquefier, comprises the clear liquid of liquefaction and the mixed liquid of liquefaction;
(6) adding the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in seeding tank, both ratios are 1:5-1:9, and the waste water produced with clear water or citric acid leaching process is settled to 26m3, seeding tank total reducing sugar is 10-13%, and adjustment total nitrogen content is 0.15-0.20%, and 121 DEG C of sterilizing 30min, when being cooled to 37-39 DEG C, press 2-5*104The amount access aspergillus niger spore of individual/ml, carries out aerlbic culture, incubation time 20-30h;
(7) adding the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in fermentor tank, both ratios are 1:5-1:9, and the waste water produced with clear water or citric acid leaching process is settled to 295-310m3, fermention medium total reducing sugar is 17-18%, lowers the temperature after 95 DEG C of sterilizing 30min, when temperature is down to 65-70 DEG C, adds the saccharifying enzyme of 80-200U/ml fermention medium;
(8) when fermention medium temperature is down to 38-40 DEG C, start to move and plant, carry out aerlbic culture;
(9) fermentation period is 55-72h, and fermentation ends acid production rate is 16.8-18.2%, and fermentation conversion rate is 96.5-100.5%;
(10) extract citric acid by " calcium salt method " or " chromatography ", obtain citric acid finished product.
Embodiment 7
With a method for wheat starch milk fermentation citric acid, its key step comprises:
(1) water and flour are in the ratio of 0.7:1-1:1 and face, slaking 1h;
(2) through cyclone separator, wheat A starch slurry is isolated;
(3) isolating wheat B starch slurry through gluten sieve, wheat A, B starch slurry concentrates in a container, mixes, and forms wheat starch breast, and wheat starch breast total sugar content is 15-20%, and total nitrogen content is 0.08-0.20%;
(4) wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, by the enzyme concentration of 20-60U/g starch, add fire resistant alpha-diastase, instant steam is utilized to be heated to 100 DEG C, maintain 5min, utilize the System and Surroundings temperature difference so that feed temperature is down to 85-95 DEG C, maintaining 1-2h, the lo-tionized liquid DE value of gained wheat starch is 25-30%;
(5) waste water that other starchy material is undertaken liquefying or utilizing citric acid leaching process to produce by patent CN104109697A by existing ripe liquefaction process liquefies, and obtains corresponding liquefier, comprises the clear liquid of liquefaction and the mixed liquid of liquefaction;
(6) adding two or more of the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in seeding tank, according to any proportion mixing, and the waste water produced with clear water or citric acid leaching process is settled to 26m3, seeding tank total reducing sugar is 7-11%, and adjustment total nitrogen content is 0.15-0.20%, and 121 DEG C of sterilizing 30min, when being cooled to 37-39 DEG C, press 2-5*104The amount access aspergillus niger spore of individual/ml, carries out aerlbic culture, incubation time 20-30h;
(7) adding two or more of the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in fermentor tank, according to any proportion mixing, and the waste water produced with clear water or citric acid leaching process is settled to 295-310m3, fermention medium total reducing sugar is 15-17%, lowers the temperature after 95 DEG C of sterilizing 30min, when temperature is down to 65-70 DEG C, adds the saccharifying enzyme of 80-200U/ml fermention medium;
(8) when fermention medium temperature is down to 38-40 DEG C, start to move and plant, carry out aerlbic culture;
(9) fermentation period is 55-65h, and fermentation ends acid production rate is 15.7-17.8%, and fermentation conversion rate is 99.7-101.9%;
(10) extract citric acid by " calcium salt method " or " chromatography ", obtain citric acid finished product.
Embodiment 8
With a method for wheat starch milk fermentation citric acid, its key step comprises:
(1) water and flour are in the ratio of 0.7:1-1:1 and face, slaking 1h;
(2) through cyclone separator, wheat A starch slurry is isolated;
(3) isolating wheat B starch slurry through gluten sieve, wheat A, B starch slurry concentrates in a container, mixes, and forms wheat starch breast, and wheat starch breast total sugar content is 20-25%, and total nitrogen content is 0.08-0.20%;
(4) wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, by the enzyme concentration of 20-60U/g starch, add fire resistant alpha-diastase, instant steam is utilized to be heated to 105 DEG C, maintain 10min, utilize the System and Surroundings temperature difference so that feed temperature is down to 85-95 DEG C, maintaining 3-4h, the lo-tionized liquid DE value of gained wheat starch is 25-35%;
(5) waste water that other starchy material is undertaken liquefying or utilizing citric acid leaching process to produce by patent CN104109697A by existing ripe liquefaction process liquefies, and obtains corresponding liquefier, comprises the clear liquid of liquefaction and the mixed liquid of liquefaction;
(6) adding two or more of the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in seeding tank, according to any proportion mixing, and the waste water produced with clear water or citric acid leaching process is settled to 26m3, seeding tank total reducing sugar is 10-13%, and adjustment total nitrogen content is 0.15-0.20%, and 121 DEG C of sterilizing 30min, when being cooled to 37-39 DEG C, press 2-5*104The amount access aspergillus niger spore of individual/ml, carries out aerlbic culture, incubation time 20-30h;
(7) adding two or more of the lo-tionized liquid of wheat starch and other liquefying starchiness raw material liquid in fermentor tank, according to any proportion mixing, and the waste water produced with clear water or citric acid leaching process is settled to 295-310m3, fermention medium total reducing sugar is 17-18%, lowers the temperature after 95 DEG C of sterilizing 30min, when temperature is down to 65-70 DEG C, adds the saccharifying enzyme of 80-200U/ml fermention medium;
(8) when fermention medium temperature is down to 38-40 DEG C, start to move and plant, carry out aerlbic culture;
(9) fermentation period is 60-75h, and fermentation ends acid production rate is 16.5-17.8%, and fermentation conversion rate is 95.3-99.6%;
(10) extract citric acid by " calcium salt method " or " chromatography ", obtain citric acid finished product.
More than implement mode only for technical conceive and the feature of the present invention are described; its object is to allow person skilled in the art understand the content of the present invention and implemented; can not limit the scope of the invention with this; all equivalences done according to spirit of the invention change or modify, and all should be encompassed in protection scope of the present invention.
Claims (10)
1. produce the method for citric acid with wheat starch milk fermentation for one kind, it is characterized in that: wheat starch breast is after pretreatment, or mix with other liquefying starchiness raw material liquid, it is mixed with seed culture medium and fermention medium, aspergillus niger spore access seed culture medium enlarged culturing, move to, in fermentor tank, obtaining finished lemon/lime acid through fermentation, extraction.
2. according to right 1 a kind of with wheat starch milk fermentation produce citric acid method, it is characterized in that: described wheat starch breast is the by product after industrial production gluten powder, the i.e. mixed solution of wheat A starch and wheat B starch, total sugar content is 15-30%, and total nitrogen content is 0.08-0.20%.
3. according to right 1 a kind of with wheat starch milk fermentation produce citric acid method, it is characterized in that: the pretreatment process of described wheat starch breast is that wheat starch breast is heated to 50-60 DEG C through plate-type heat exchanger, mix with ��-fire resistant alpha-diastase, ��-fire resistant alpha-diastase amount of adding is 20-60U/g starch, through being steam heated to 100-110 DEG C, insulation 5-15min, is then cooled to 80-95 DEG C, maintains 2-5h.
4. according to right 1 or 3 a kind of with wheat starch milk fermentation produce citric acid method, it is characterised in that: after described pre-treatment, the DE value of wheat starch breast is 25-40%.
5. according to right 1 a kind of with wheat starch milk fermentation produce citric acid method, it is characterized in that: other starchy material described is one or more in Semen Maydis powder, sorghum flour, Tapioca Starch, starch, sweet potato powder, pretreated total sugar content is 15-30%.
6. according to right 1 a kind of with wheat starch milk fermentation produce citric acid method, it is characterized in that: described seed culture medium by wheat starch breast or with other starchy material by 1:1-1:9 mixed preparing, and surely hold with clear water or other water source, total sugar content is 7-13%, have when needing, additional sulfuric acid ammonium, bean cake powder or cottonseed are as nitrogenous source, and seed culture medium total nitrogen content is 0.15-0.30%.
7. according to right 6 a kind of with wheat starch milk fermentation produce citric acid method, it is characterised in that: other described water source be in citric acid leaching process produce waste water.
8. according to right 1 a kind of with wheat starch milk fermentation produce citric acid method, it is characterized in that: described with wheat starch breast or with after other starchy material mixing citric acid preparation fermention medium, saccharifying enzyme need to be added in the fermentation medium, saccharifying enzyme add-on is 80-200U/ml fermention medium, after sterilizing, adding saccharifying enzyme when temperature is down to 65-70 DEG C, carry out saccharification in fermention medium temperature-fall period, after saccharification, feed liquid DE value is 60-90%.
9. according to right 1 or 8 a kind of with wheat starch milk fermentation produce citric acid method, it is characterised in that: described fermention medium total sugar content is 15-18%, and total nitrogen content is 0.08-0.12%.
10. according to right 1 a kind of with wheat starch milk fermentation produce citric acid method, it is characterized in that: described aspergillus niger spore for using wheat starch breast as fermentation raw material, the adaptation that screening obtains and wheat starch milk fermentation, the aspergillus niger strain that stability is high.
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| CN111607622A (en) * | 2020-05-15 | 2020-09-01 | 山东省食品发酵工业研究设计院 | Process method for producing 3-hydroxy butanone by using wheat B starch |
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