CN105613049A - Method for cultivating black fungus strains by aid of decomposable pine and cypress sawdust - Google Patents
Method for cultivating black fungus strains by aid of decomposable pine and cypress sawdust Download PDFInfo
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- CN105613049A CN105613049A CN201610134506.0A CN201610134506A CN105613049A CN 105613049 A CN105613049 A CN 105613049A CN 201610134506 A CN201610134506 A CN 201610134506A CN 105613049 A CN105613049 A CN 105613049A
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- pine
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- black fungus
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- mycelia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention relates to a method for cultivating black fungus strains by the aid of decomposable pine and cypress sawdust. The method includes (1), collecting black fungi grown on pine trees, transferring the black fungi in culture media and cultivating the black fungi in the culture media in an isolated manner; (2), transferring the black fungi in ordinary culture media and cultivating the black fungi in the ordinary culture media; (3), inoculating the black fungi in fungus bags with culture media with the pine and cypress sawdust and cultivating strains until the fungus bags are filled with the grown strains; (4), transferring the strains from the fungus bags into ordinary PDA (potato dextrose agar) culture media. The method has the advantages that processes are simple, the cost is low, the pine and cypress sawdust can be sufficiently utilized for cultivating the black fungi as compared the cultivated black fungus strains to conventional black fungus strains, wastage of a large quantity of pine and cypress sawdust can be prevented, and the method for cultivating the black fungi in China is unique and has an excellent application prospect.
Description
Technical field
The invention belongs to black fungus bacterial classification field, in particular to the cultivating method of the black fungus bacterial classification of a kind of decomposable asymmetric choice net pine and cypress trees bits.
Background technology
China is that edible fungus culturing big country, particularly cultivation of auricularia auricula are extensive in China region, certainly reaches the cultivation that there is black fungus in north in the south. But the cultivation wood chip used of black fungus is all the wood chip of non-pine and cypress class, the wood chip of a large amount of pine tree of China and cypress can only be by heap fermentation for many years at present, just can carry out culture and utility after removing in wood chip the pine and cypress resin contained, thus cause the waste of this type of wood chip a large amount of. Therefore how to cultivate that can to decompose the black fungus bacterial classification utilizing pine and cypress wood chip be one of the key factor solving the waste of this type of wood chip.
Summary of the invention
Technical problem to be solved by this invention is to provide the cultivating method of the black fungus bacterial classification of a kind of decomposable asymmetric choice net pine and cypress trees bits, the method technique is simple, cost is low, the black fungus bacterial classification of cultivation is compared with conventional black fungus bacterial classification, there is the advantage that can make full use of pine tree and cypress wood chip cultivating black fungus, avoid a large amount of pine and cypress wood chip to waste, in China, cultivation of auricularia auricula kind has uniqueness, has a good application prospect.
The cultivating method of the black fungus bacterial classification of a kind of decomposable asymmetric choice net pine and cypress trees bits of the present invention, comprising:
(1) bacterial context getting the wild black fungus sporophore being grown on pine and cypress tree or the spore launched, be positioned on the substratum prepared in advance under open environment, substratum is positioned over the indoor cultivation 7��8 days of 25��26 DEG C subsequently, treats that mycelia grows;
(2) mycelia that step (1) obtains is transferred on common PDA substratum, subsequently substratum is positioned over the indoor cultivation 7��8 days of 25��26 DEG C, treats that mycelia grows;
(3) mycelia that step (2) obtains is transferred in pine and cypress tree sawdust medium bacterium bag (35cm �� 17cm) prepared in advance, subsequently bacterium bag is positioned over indoor 30-40 days of 25��26 DEG C, treats that mycelia is covered with;
(4) mycelia step (3) obtained is considered to be worth doing bacterium bag from pine and cypress trees and is transferred in common PDA substratum, is positioned over the indoor cultivation 7��8 days of 25��26 DEG C.
Substratum in described step (1) is PDA substratum.
Common PDA substratum in described step (2) consist of potato 200g filtration to boil water, glucose 20g and agar 20g.
Pine and cypress tree sawdust medium in described step (3) consist of pine and cypress wood chip 78wt%, wheat bran 20wt%, sugar 1wt% and gypsum 1wt%.
Useful effect
Technique of the present invention is simple, cost is low, and the black fungus bacterial classification of cultivation, compared with conventional black fungus bacterial classification, has the advantage that can make full use of pine tree and cypress wood chip cultivating black fungus, avoid a large amount of pine and cypress wood chip to waste, in China, cultivation of auricularia auricula kind has uniqueness, has a good application prospect.
Accompanying drawing explanation
Fig. 1 is wild pine tree black fungus sporophore;
Fig. 2 is the black fungus bacterial classification of decomposable asymmetric choice net pine and cypress trees bits.
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further. Limit the scope of the invention it will be understood that these embodiments are only not used in for illustration of the present invention. In addition it will be understood that after having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
(1) the bacterial context spore that is a little or that launch getting the wild black fungus sporophore being grown on pine and cypress tree is a small amount of, it is positioned on the PDA substratum prepared in advance under open environment, the indoor cultivation 7 days that substratum is positioned over 25 DEG C subsequently, treats that mycelia grows;
(2) mycelia that step (1) obtains is transferred on common PDA substratum (potato 200g filtration to boil water, glucose 20g and agar 20g), subsequently substratum is positioned over the indoor cultivation 7 days of 25 DEG C, treats that mycelia grows;
(3) mycelia that step (2) obtains is transferred in pine and cypress tree sawdust medium (pine and cypress wood chip 78wt%, wheat bran 20wt%, sugar 1wt% and gypsum 1wt%) the bacterium bag (35cm �� 17cm) prepared in advance, the indoor 30 days that bacterium bag is positioned over 25 DEG C subsequently, treat that mycelia is covered with;
(4) mycelia step (3) obtained is considered to be worth doing bacterium bag from pine and cypress trees and is transferred in common PDA substratum, is positioned over the indoor cultivation 7 days of 25 DEG C.
Embodiment 2
(1) the bacterial context spore that is a little or that launch getting the wild black fungus sporophore being grown on pine and cypress tree is a small amount of, it is positioned on the PDA substratum prepared in advance under open environment, the indoor cultivation 8 days that substratum is positioned over 26 DEG C subsequently, treats that mycelia grows;
(2) mycelia that step (1) obtains is transferred on common PDA substratum (potato 200g filtration to boil water, glucose 20g and agar 20g), subsequently substratum is positioned over the indoor cultivation 7 days of 25 DEG C, treats that mycelia grows;
(3) mycelia that step (2) obtains is transferred in pine and cypress tree sawdust medium (pine and cypress wood chip 78wt%, wheat bran 20wt%, sugar 1wt% and gypsum 1wt%) the bacterium bag (35cm �� 17cm) prepared in advance, the indoor 40 days that bacterium bag is positioned over 26 DEG C subsequently, treat that mycelia is covered with;
(4) mycelia step (3) obtained is considered to be worth doing bacterium bag from pine and cypress trees and is transferred in common PDA substratum, is positioned over the indoor cultivation 8 days of 26 DEG C.
Claims (4)
1. a cultivating method for the black fungus bacterial classification of decomposable asymmetric choice net pine and cypress trees bits, comprising:
(1) bacterial context getting the wild black fungus sporophore being grown on pine and cypress tree or the spore launched, be positioned on the substratum prepared in advance under open environment, substratum is positioned over the indoor cultivation 7��8 days of 25��26 DEG C subsequently, treats that mycelia grows;
(2) mycelia that step (1) obtains is transferred on common PDA substratum, subsequently substratum is positioned over the indoor cultivation 7��8 days of 25��26 DEG C, treats that mycelia grows;
(3) mycelia that step (2) obtains is transferred in the pine and cypress tree sawdust medium bacterium bag prepared in advance, subsequently bacterium bag is positioned over indoor 30-40 days of 25��26 DEG C, treats that mycelia is covered with;
(4) mycelia step (3) obtained is considered to be worth doing bacterium bag from pine and cypress trees and is transferred in common PDA substratum, is positioned over the indoor cultivation 7��8 days of 25��26 DEG C.
2. the cultivating method of the black fungus bacterial classification of a kind of decomposable asymmetric choice net pine and cypress trees according to claim 1 bits, it is characterised in that: the substratum in described step (1) is PDA substratum.
3. the cultivating method of black fungus bacterial classification of a kind of decomposable asymmetric choice net pine and cypress trees according to claim 1 bits, it is characterised in that: the common PDA substratum in described step (2) consist of potato 200g filtration to boil water, glucose 20g and agar 20g.
4. the cultivating method of black fungus bacterial classification of a kind of decomposable asymmetric choice net pine and cypress trees according to claim 1 bits, it is characterised in that: the pine and cypress tree sawdust medium in described step (3) consist of pine and cypress wood chip 78wt%, wheat bran 20wt%, sugared 1wt% and gypsum 1wt%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610134506.0A CN105613049B (en) | 2016-03-09 | 2016-03-09 | A kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust |
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| CN201610134506.0A CN105613049B (en) | 2016-03-09 | 2016-03-09 | A kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust |
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| CN105613049A true CN105613049A (en) | 2016-06-01 |
| CN105613049B CN105613049B (en) | 2018-12-14 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901589A (en) * | 2018-04-18 | 2018-11-30 | 上海沃施生物科技有限公司 | A kind of separation method of wild delicious lactarius strain |
| CN113174338A (en) * | 2021-05-28 | 2021-07-27 | 广西壮族自治区农业科学院 | Black fungus tissue isolation culture medium and application thereof |
Citations (4)
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|---|---|---|---|---|
| CN101830747A (en) * | 2010-03-03 | 2010-09-15 | 上海市农业科学院 | Culture medium for use in artificial culture of Tibetan Yatung wild black fungi |
| CN102440150A (en) * | 2011-10-14 | 2012-05-09 | 姚安农哈哈食用菌开发有限公司 | Processing method for planting edible fungus by replacing hardwood forest wood chips with pine wood chips |
| CN105254407A (en) * | 2015-10-01 | 2016-01-20 | 西藏大学农牧学院 | Black fungus cultivation material and method |
| CN105265164A (en) * | 2014-07-22 | 2016-01-27 | 王健 | Method for cultivating edible mushrooms by pine/cedar/eucalyptus wood chips |
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2016
- 2016-03-09 CN CN201610134506.0A patent/CN105613049B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101830747A (en) * | 2010-03-03 | 2010-09-15 | 上海市农业科学院 | Culture medium for use in artificial culture of Tibetan Yatung wild black fungi |
| CN102440150A (en) * | 2011-10-14 | 2012-05-09 | 姚安农哈哈食用菌开发有限公司 | Processing method for planting edible fungus by replacing hardwood forest wood chips with pine wood chips |
| CN105265164A (en) * | 2014-07-22 | 2016-01-27 | 王健 | Method for cultivating edible mushrooms by pine/cedar/eucalyptus wood chips |
| CN105254407A (en) * | 2015-10-01 | 2016-01-20 | 西藏大学农牧学院 | Black fungus cultivation material and method |
Non-Patent Citations (3)
| Title |
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| 丁湖广: "黑木耳栽培技术和方法的研究", 《林业科技通讯》 * |
| 夏温澍: "黑木耳松树锯末菌种及其栽培", 《食用菌》 * |
| 范莉莉: "利用松、杉木屑栽培食用菌技术", 《农家之友》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901589A (en) * | 2018-04-18 | 2018-11-30 | 上海沃施生物科技有限公司 | A kind of separation method of wild delicious lactarius strain |
| CN113174338A (en) * | 2021-05-28 | 2021-07-27 | 广西壮族自治区农业科学院 | Black fungus tissue isolation culture medium and application thereof |
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| CN105613049B (en) | 2018-12-14 |
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