CN105596961A - Traditional Chinese medicine preparation for treating chronic obstructive pulmonary disease and preparation method of traditional Chinese medicine preparation - Google Patents
Traditional Chinese medicine preparation for treating chronic obstructive pulmonary disease and preparation method of traditional Chinese medicine preparation Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine preparation for treating a chronic obstructive pulmonary disease and a preparation method of the traditional Chinese medicine preparation. The traditional Chinese medicine preparation is prepared from the blackberry lily, gomphrena globosa, lycoris radiate, fructus amomi rotundus, rauvolfia verticillate and plumeria rubra. The preparation method comprises the following steps: cleaning, airing, crushing, screening and mixing various raw materials; extracting the raw materials by a supercritical CO2 extraction technology, collecting extracted volatile oil, carrying out warm-soaking extraction with ethanol, and filtering and collecting an extract solution; adding water for reflux extraction, filtering and pumping the extract solution into an ultrasonic standing wave liquid-phase preparation chromatography; and separating and purifying the extract solution to obtain the target traditional Chinese medicine preparation. The medicine disclosed by the invention has the efficacies of moistening the lung, preserving yin, clearing heat, cooling blood, freeing lung, relieving asthma, relieving a sore throat and expelling pus, and is significant in curative effect, low in price, convenient to use, free of a toxic or side effect and free of dependency. The injury on hepatic and renal functions caused by the western medicine can be reduced by the treatment mode of the traditional Chinese medicine preparation; and improvement of the disease is accelerated, so that the target of treating both symptoms and root causes is achieved.
Description
Technical field
The present invention relates to the field of Chinese medicines, specifically a kind of Chinese medicine preparation and preparation thereof for the treatment of chronic obstructive pulmonary diseaseMethod.
Background technology
COPD is a kind of important chronic respiratory disease, and number of patients is many, and case fatality rate is high.Because it slowly carries out sexual development, have a strong impact on patient's labour capacity and quality of life. Chronic obstructive pulmonary diseasePatient is after acute attack stage, though clinical symptoms are alleviated to some extent, its PFT is still continuing deterioration, andDue to the reduction of self-defense and immunologic function and the impact of extraneous various adverse factors, often outbreak repeatedly,And produce gradually various Cardiopulmonary Complications. This disease is a kind of destructive PUD D, with not exclusively reversibleFlow limitation is the disease of feature, flow limitation be conventionally carry out sexual development and with lung to deleterious particle or gasAbnormal inflammatory reaction is relevant. Although chronic obstructive pulmonary disease is the disease of air flue, to the systematic influence of whole body alsoCan not be ignored. This sick cardinal symptom performance taste is (1) chronic cough, and obviously, have night in normal daystart coughParoxysmal cough or expectoration; (2) expectoration, is generally white mucus or serosity frothy sputum, occasionally can be with the trace of blood, clearMorning, expectoration was more. Acute attack stage amount of expectoration increases, and can have purulent sputum; (3) breathe hard or have difficulty in breathing. In early daysIn the time of labour, occur, after increase the weight of gradually so that even also feel to breathe hard when rest in daily routines, be chronic resistanceThe significant symptom of plug property tuberculosis; (4) pant with uncomfortable in chest, some patients were is severe patient or acute adding particularlyWhen heavy, occur panting; (5) patients with terminal has Body weight loss, anorexia etc.
At present mostly the medicine for the treatment of chronic obstructive pulmonary disease is that index do not effect a permanent cure, although clinical symptoms alleviate to some extent,But PFT still worsens continuing, and due to the reduction of self-defense and immunologic function and extraneously variously haveThe impact of noxa element, often outbreak repeatedly, brings great misery to patient, and the traditional Chinese medical science is past in treatment chronic diseaseToward being good at removing focus from root, taking Yin nourishing and lung moistening, tonify the kidney to improve inspiration as main, alleviate patient's slight illness, therebyReach the effect for the treatment of.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease and preparation method thereof,To solve the problem proposing in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
Treat a Chinese medicine preparation for chronic obstructive pulmonary disease, be prepared from by the raw material of following weight portion: blackberry lily25～35 parts, 30～40 parts of globe amaranths, 18～24 parts of short-tube lycoris, 16～20 parts, cardamom, devilpepper 14～18Part, 20～30 parts of frangipanis.
A preparation method for the Chinese medicine preparation of described treatment chronic obstructive pulmonary disease, concrete steps are as follows:
(1) above-mentioned each raw material is cleaned and dried respectively, take each raw material by proportioning, pulverize with pulverizer respectivelyAfter, cross 300～360 mesh sieves, fully mix;
(2) raw material mixing is adopted to supercritical carbon dioxide extraction method, 54～56 DEG C of extraction temperature, extractionPressure power 4～5MPa, extraction time 2～3h, collects the volatile oil of extraction, obtains extract A, for subsequent use;
(3) be solvent by extract A with concentration 90～95% ethanol, at 75～80 DEG C, warm lixiviate is got, and carriesGetting number of times is 3～5 times, and each extraction time is 2～3h, and each solvent load is 8～10 of raw material gross weightDoubly, filter, collect extract, extract reclaims ethanol, and concentrate drying, obtains extract B;
(4) extract B is added water refluxing extraction, extraction time is 2～4 times, each extraction time is 2～3h, each water consumption is 6～8 times of raw material gross weight, filters, and obtains extract;
(5) extract is pumped in ultrasonic standing wave preparative liquid chromatogram, ultrasonic standing wave power 3.0～5.0KW,Frequency 900～1000kHz, flow velocity 200～300mL/min, UV-detector wavelength 280nm, separation and purification,Obtain target Chinese medicine preparation.
Compared with prior art, the invention has the beneficial effects as follows: medicine of the present invention have moisten the lung and nourish yin, heat-clearing is coolBlood, freeing lung and relieving asthma, the effect such as relieve sore throat apocenosis, and evident in efficacy, cheap, easy to use, nontoxic secondary workWith, no dependence, the mode that this Chinese medicine preparation adopts is treated and can be reduced the damage of Western medicine to patient's hepatic and renal function,Accelerate the improvement of the state of an illness, the object that reaching takes stopgap measures effects a permanent cure.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clearly and completelyDescribe, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art obtain not making under creative work prerequisiteThe every other embodiment obtaining, belongs to the scope of protection of the invention.
Embodiment 1
The Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease, is prepared from by the raw material of following weight portion: blackberry lily 25Part, 40 parts of globe amaranths, 18 parts of short-tube lycoris, 20 parts, cardamom, 14 parts of devilpeppers, 30 parts of frangipanis.
The preparation process of Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease in above-described embodiment is as follows:
(1) above-mentioned each raw material is cleaned and dried respectively, take each raw material by proportioning, pulverize with pulverizer respectivelyAfter, cross 350 mesh sieves, fully mix;
(2) raw material mixing is adopted to supercritical carbon dioxide extraction method, 54 DEG C of extraction temperature, extraction is pressedPower 5MPa, extraction time 3h, collects the volatile oil of extraction, obtains extract A, for subsequent use;
(3) be solvent by extract A with concentration 90～95% ethanol, at 75 DEG C, warm lixiviate is got, and extracts inferiorNumber is 5 times, and each extraction time is 3h, and each solvent load is 8 times of raw material gross weight, filters, and receivesCollection extract, extract reclaims ethanol, and concentrate drying, obtains extract B;
(4) extract B is added water refluxing extraction, extraction time is 2 times, each extraction time is 3h, everyInferior water consumption is 6 times of raw material gross weight, filters, and obtains extract;
(5) extract is pumped in ultrasonic standing wave preparative liquid chromatogram to ultrasonic standing wave power 4.0KW, frequency1000kHz, flow velocity 200mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine systemAgent.
Embodiment 2
The Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease, is prepared from by the raw material of following weight portion: blackberry lily 30Part, 35 parts of globe amaranths, 21 parts of short-tube lycoris, 18 parts, cardamom, 16 parts of devilpeppers, 25 parts of frangipanis.
The preparation process of Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease in above-described embodiment is as follows:
(1) above-mentioned each raw material is cleaned and dried respectively, take each raw material by proportioning, pulverize with pulverizer respectivelyAfter, cross 350 mesh sieves, fully mix;
(2) raw material mixing is adopted to supercritical carbon dioxide extraction method, 55 DEG C of extraction temperature, extraction is pressedPower 4.5MPa, extraction time 2.5h, collects the volatile oil of extraction, obtains extract A, for subsequent use;
(3) be solvent by extract A with concentration 95% ethanol, at 78 DEG C, warm lixiviate is got, and extraction time is4 times, each extraction time is 2.5h, and each solvent load is 9 times of raw material gross weight, filters, and collection is carriedGet liquid, extract reclaims ethanol, and concentrate drying, obtains extract B;
(4) extract B is added water refluxing extraction, extraction time is 3 times, each extraction time is 2.5h,Each water consumption is 7 times of raw material gross weight, filters, and obtains extract;
(5) extract is pumped in ultrasonic standing wave preparative liquid chromatogram to ultrasonic standing wave power 4.0KW, frequency1000kHz, flow velocity 250mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine systemAgent.
Embodiment 3
The Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease, is prepared from by the raw material of following weight portion: blackberry lily 35Part, 30 parts of globe amaranths, 24 parts of short-tube lycoris, 16 parts, cardamom, 18 parts of devilpeppers, 20 parts of frangipanis.
The preparation process of Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease in above-described embodiment is as follows:
(1) above-mentioned each raw material is cleaned and dried respectively, take each raw material by proportioning, pulverize with pulverizer respectivelyAfter, cross 350 mesh sieves, fully mix;
(2) raw material mixing is adopted to supercritical carbon dioxide extraction method, 56 DEG C of extraction temperature, extraction is pressedPower 4MPa, extraction time 2h, collects the volatile oil of extraction, obtains extract A, for subsequent use;
(3) be solvent by extract A with concentration 95% ethanol, at 80 DEG C, warm lixiviate is got, and extraction time is3 times, each extraction time is 2h, and each solvent load is 10 times of raw material gross weight, filters, and collection is carriedGet liquid, extract reclaims ethanol, and concentrate drying, obtains extract B;
(4) extract B is added water refluxing extraction, extraction time is 4 times, each extraction time is 2h, everyInferior water consumption is 8 times of raw material gross weight, filters, and obtains extract;
(5) extract is pumped in ultrasonic standing wave preparative liquid chromatogram to ultrasonic standing wave power 4.0KW, frequency1000kHz, flow velocity 300mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine systemAgent.
The anti-chronic obstructive pulmonary disease experiment of embodiment 5 Chinese medicine preparation
Method: the female BALB/c mouse of SPF level, 18-20g, is divided into Normal group, model contrast at randomGroup, drug-treated group (Chinese medicine preparation, Compound I H and compound IV 10mg/kg, gastric infusion), every group10. Mouse is placed in 4L container, sootiness every day (Guangdong board cigarette one red shell, tar content is less than 16mg),One Friday time, surrounding continuously, first 1 hour gastric infusion of sootiness every day, mouse after last sootiness 1 hour,With sodium phenobarbital anesthesia, with PBS buffer solution (pH=7.0) the lung perfusion of 0.6mL precooling, rinse 3Inferior, sucking-off BAL fluid (BALF), the centrifugal 10min of 250xg, supernatant be used for analyzing TNF α,The content of RANTES. Cell is resuspended with PBS buffer solution, and counting is used Rui Shi-Giemsa staining after cell smear,Analyze the variation of inflammatory cell in BALF.
The preparation of Chinese medicine preparation: get respectively the medicine of embodiment 1, embodiment 2 and embodiment 3, use distilled waterBe diluted to the liquid of 10mg/L.
Experimental example 1 Chinese medicine preparation generates inflammatory mediator in COPD mouse bronchial bronchoalveolar lavage fluid (BALF)Inhibitory action
(1) Chinese medicine preparation generates inhibitory action to TNF α in COPD mouse BALF
The startup factor in the pathogenic process that front inflammatory cytokine TNF α is COPD, COPD patient's TNF αLevel is higher than normal person, and the bronchial epithelial cell of cultivation contacts with the smog of cigarette can TNF secretion α, TNF αCan impel neutrophil leucocyte to take off particle, mucomembranous cell hyperplasia and hypersecretion are played in induction, increase epithelial cell IL-8Generate, increase Macrophage Matrix Metalloproteinase and generate, promote airway hyperreactivity. This experiment purpose isInvestigate the impact that Chinese medicine preparation generates TNF α in COPD mouse BALF, the results are shown in Table 1.
The impact that table 1 Chinese medicine preparation generates TNFQ in COPD mouse BALF
| Group | Dosage (mg/kg) | The content (pg/ml) of TNF α in COPD mouse BALF |
| Normal group | 6.9 | |
| Model control group | 28.4# | |
| Embodiment 1 | 10 | 11.6* |
| Embodiment 2 | 10 | 11.2 |
| Embodiment 3 | 10 | 11.5 |
Note: * P < 0.05, compared with model control group; #P < 0.05, compared with Normal group. N=10.
Result: Chinese medicine preparation to COPD model mice gastric infusion, can significantly reduce little under 10mg/L concentrationThe generation of TNF α in mouse BALF.
(2) Chinese medicine preparation is to RANTES secretion inhibition in COPD mouse BALF
The migration of inflammatory cell is subject to the adjusting of chemotactic factor (CF), and various Constituent cell core inflammatory cells all can be secreted thisA little small molecular proteins, in COPD pathogenic process, RANTES secretion increases, chemotactic Monocytes/Macrophages, T lymphCell, acidophic cell. This experiment purpose is to investigate Chinese medicine preparation to KANTES in COPD mouse BALFThe impact of secretion, the results are shown in Table 2.
The impact of table 2 Chinese medicine preparation on RANTES secretion in COPD mouse BALF
| Group | Dosage (mg/kg) | The content (pg/ml) of TNF α in COPD mouse BALF |
| Normal group | 11.6 | |
| Model control group | 75.2# | |
| Embodiment 1 | 10 | 21.2* |
| Embodiment 2 | 10 | 21.0 |
| Embodiment 3 | 10 | 21.3 |
Note: * P < 0.05, compared with model control group; #P < 0.01, compared with Normal group. N=10.
Result: Chinese medicine preparation to COPD model mice gastric infusion, can reduce mouse under 10mg/L concentrationThe secretion of RANTES in BALF.
Experimental example 2 Chinese medicine preparations are to inhibitory action that in COPD mouse BALF, inflammatory cell is raised
(l) Chinese medicine preparation is to inhibitory action that in COPD mouse BALF, neutrophil leucocyte is raised
Neutrophil leucocyte has important function in COPD morbidity, and it can discharge two kinds of serine proteases,Elastoser and half moon bright propylhomoserin proteinase-3, induced animal produces the pathological change of mankind's pulmonary emphysema sample. NeutralGranulocyte life is of short duration, and the process that air flue and crossing gap chamber are recruited in its circulation is very rapid. Pathological studyProve that in some COPD patient bronchial tissue, neutrophil leucocyte number increases relevant with the degree of airflow obstruction.In the COPD patient airway of smoking, neutrophil leucocyte number increases, and particularly those are with chronic bronchitisPatient. Affecting the principal element that in COPD Patients with Lung, neutrophil leucocyte is raised is that IL-8 chemotactic activity strengthens. ThisExperiment purpose is to investigate Chinese medicine preparation to the impact that in COPD mouse BALF, neutrophil leucocyte is raised, and the results are shown inTable 3.
Table 3 Chinese medicine preparation is on impact that in COPD mouse BALF, neutrophil leucocyte is raised
| Group | Dosage (mg/kg) | The content (pg/ml) of TNF α in COPD mouse BALF |
| Normal group | 0.15 | |
| Model control group | 1.78# | |
| Embodiment 1 | 10 | 0.30* |
| Embodiment 2 | 10 | 0.28 |
| Embodiment 3 | 10 | 0.29 |
Note: * P < 0.05; #P < 0.01, compared with Normal group; N=10.
Result: Chinese medicine preparation to COPD model mice gastric infusion, can reduce mouse BALF under 10mg/LRaising of middle neutrophil leucocyte.
(2) Chinese medicine preparation is to inhibitory action that in c0PD mouse BALF, macrophage is raised
In smoker and COPD Patients with Lung, macrophage compared with normal crowd level increases, and is gathered in alveolar, thin moreBronchus and small airway. Alveolus wall macrophage number and light moderate pulmonary emphysema and COPD patient's small airwayDisease degree is proportionate. Lesion tissue and damage location can be seen the slow progress of COPD and chronic disease and hugeThe long-term increase of phagocyte is parallel. Macrophage may cause elastic fibrous tissue degraded by release matrix metalloproteinasesAbility increases extremely, brings out emophysematous generation. This experiment purpose is to investigate Chinese medicine preparation to COPD mouseThe impact that in BALF, macrophage is raised, the results are shown in Table 4.
Table 4 Chinese medicine preparation is on impact that in COPD mouse BALF, macrophage is raised
Note: * P < 0.05, compared with model control group; #P < 0.01, compared with Normal group, n=10.
Result: Chinese medicine preparation to COPD model mice gastric infusion, can significantly reduce little under 10mg/L concentrationIn mouse BALF, macrophage raises.
Conclusion: Chinese medicine preparation is gastric infusion under 10mg/L concentration, to the mouse chronic obstructive of cigarette inductionThe generation of the inflammatory mediator in tuberculosis process and the secretion of chemotactic factor (CF) have remarkable inhibitory action, to inflammatory cellInfiltration has significant inhibition, and chronic obstructive pulmonary disease is had significant therapeutic effect.
The clinical statistics data that patient uses medicine of the present invention below
1, physical data
Accept out-patient's 400 examples for medical treatment, patient is divided into observation group and control group at random, the wherein male sex's 220 examples,Women's 180 examples, M-F 1.2:1,45 years old mean age, two groups of patient ages, sex, course of disease etc.Every basic document is basic identical, difference not statistically significant (P > 0.05).
2, methods for the treatment of
The particle that observation group prepares according to medicine embodiment 2 of the present invention, boiling water is taken after mixing it with water, every day 3 times, each oneBag 15th is a course for the treatment of.
Control group, according to conventional western medicine method, carries out symptomatic treatment to it, within 15 days, is an observation period.
3, efficacy assessment standard
Cure: the transference cures such as expiratory dyspnea, expectoration, cough, heavy breathing toot sound, it is normal that blood gas index recovers, bodyIt is normal that matter is recovered, and every inspection is all normal;
Take a turn for the better: the symptoms such as expiratory dyspnea, expectoration, cough, heavy breathing toot sound alleviate, blood gas index take a turn for the better, physique byGradually take a turn for the better, indices all recovers to some extent;
Do not heal: various clinical symptoms are without any improvement, even increase the weight of.
4, observation group's result statistics
Control group result statistics
Take two groups of patients', two courses for the treatment of Comparison of therapeutic situation
| Group | Number of cases | Cure rate | Improvement rate | Rate does not heal | Total effective rate |
| Observation group | 200 | 80 | 19 | 1 | 99 |
| Control group | 200 | 40 | 39 | 21 | 79 |
Can find by the treatment total effective rate that contrasts two groups of patients, observation group is apparently higher than control group, thusIllustrate, Chinese medicine preparation of the present invention has good clinical effectiveness in treatment chronic obstructive pulmonary disease, can effectively improveThe cure rate for the treatment of, is worth promoting in clinical practice.
Model case
Case 1: field, man, 51 years old, patient's cough, expectoration were had a surplus March, showing with heating when seriousResemble, after activity, have phenomenon uncomfortable in chest, that pant, patient goes to hospital to check, is diagnosed as chronic obstructiveTuberculosis, patient's oral western medicine about two weeks, transference cure, but how long do not have, patient's cough, expectoration,The phenomenon of breathing hard recurs again, and patient determines to adopt the methods for the treatment of of Chinese medicine, in the use embodiment of the present invention 2Medicine preparation, took after the course for the treatment of, and patient coughs and obviously alleviates, and asthma phenomenon is also eased, due to patientPhysique is poor, for consolidating drug effect, continues two courses for the treatment of of drug administration, and other symptoms such as cough, expectoration disappear completelyLose, physique also increases, uncomfortable in chest, and the phenomenon of breathing hard disappears, and removes examination in hospital, and indices all shows normally,Have no recurrence, recovery from illness.
Case 2: leaf, man, 58 years old, patient accompanied shortness of breath because of cough phlegm and has a surplus March, since patient's idiopathyThe state of mind is not good, and physique also declines gradually, and patient itself has chronic bronchitis history, and conditions of patients increases the weight of,Occurred breathing hard, the phenomenon of palpitaition, remove examination in hospital, be finally diagnosed as chronic obstructive pulmonary disease, patient adoptsThe Chinese medicine preparation of the embodiment of the present invention 2, took after the course for the treatment of, and the phenomenon of coughing, breathe hard is clearly better, spiritState is also taken a turn for the better, and continues to take a course for the treatment of, and patient coughs, breathes hard, palpitaition phenomenon disappears completely, itsHe is also taken a turn for the better at clinical symptoms, and physique also strengthens to some extent, goes hospital check, indices all to show normally,Recovery from illness.
Case 3: Lee, female, 42 years old, patient, because of cough, the admission examination of breathing hard, was finally diagnosed as chronic resistancePlug property tuberculosis, patient adopts the method for Chinese medicinal of the embodiment of the present invention 2, takes after the course for the treatment of, and cough subtractsGently, amount of expectoration reduces, and continues to take a course for the treatment of afterwards, and patient's cough, the phenomenon of breathing hard disappear completely, and physique also hasInstitute strengthens, and patient goes hospital's check, every inspection normal, recovery from illness.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned example embodiment, andAnd in the situation that not deviating from spirit of the present invention or essential characteristic, can realize this with other concrete formBright. Therefore, no matter from which point, all should regard embodiment as exemplary, and be non-limiting, scope of the present invention is limited by claims instead of above-mentioned explanation, is therefore intended to want dropping on rightAll changes in the implication and the scope that are equal to important document of asking are included in the present invention.
Claims (2)
1. a Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease, is characterized in that, is prepared from by the raw material of following weight portion: 25～35 parts of blackberry lilys, 30～40 parts of globe amaranths, 18～24 parts of short-tube lycoris, 16～20 parts, cardamom, 14～18 parts of devilpeppers, 20～30 parts of frangipanis.
2. a preparation method for the Chinese medicine preparation for the treatment of chronic obstructive pulmonary disease as claimed in claim 1, is characterized in that, concrete steps are as follows:
(1) above-mentioned each raw material is cleaned and dried respectively, take each raw material by proportioning, after pulverizing with pulverizer respectively, cross 300～360 mesh sieves, fully mix;
(2) raw material mixing is adopted to supercritical carbon dioxide extraction method, 54～56 DEG C of extraction temperature, extracting pressure 4～5MPa, extraction time 2～3h, collects the volatile oil of extraction, obtains extract A, for subsequent use;
(3) be solvent by extract A with concentration 90～95% ethanol, at 75～80 DEG C, warm lixiviate is got, extraction time is 3～5 times, each extraction time is 2～3h, and each solvent load is 8～10 times of raw material gross weight, filters, collect extract, extract reclaims ethanol, and concentrate drying, obtains extract B;
(4) extract B is added water refluxing extraction, extraction time is 2～4 times, and each extraction time is 2～3h, and each water consumption is 6～8 times of raw material gross weight, filters, and obtains extract;
(5) extract is pumped in ultrasonic standing wave preparative liquid chromatogram, ultrasonic standing wave power 3.0～5.0KW, frequency 900～1000kHz, flow velocity 200～300mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine preparation.
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| CN110916241A (en) * | 2019-11-20 | 2020-03-27 | 许达勇 | Nicotine aerosol and preparation method thereof |
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| CN101757289A (en) * | 2008-12-24 | 2010-06-30 | 天津中新药业集团股份有限公司乐仁堂制药厂 | Traditional Chinese medicine ointment for treating gastrointestinal diseases and preparation method thereof |
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| CN101757289A (en) * | 2008-12-24 | 2010-06-30 | 天津中新药业集团股份有限公司乐仁堂制药厂 | Traditional Chinese medicine ointment for treating gastrointestinal diseases and preparation method thereof |
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| 谭兴贵等: "《中国食物药用大典》", 31 July 2013, 西安交通大学出版社 * |
| 高振等: "近10 年慢性阻塞性肺病中医证治用药规律的文献研究", 《中国实验方剂学杂志》 * |
Cited By (1)
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| CN110916241A (en) * | 2019-11-20 | 2020-03-27 | 许达勇 | Nicotine aerosol and preparation method thereof |
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