CN105548020A - Full-automatic multichannel biochemical detector and detection method thereof - Google Patents
Full-automatic multichannel biochemical detector and detection method thereof Download PDFInfo
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
The invention discloses a full-automatic multichannel biochemical detector and a detection method thereof. The biochemical detector comprises a pedestal, a plurality of detection units, and a control device. Each detection unit is arranged on the pedestal, and comprises a multi-wavelength light source device, and a reaction position which is used for placing a reaction device. The multi-wavelength light source device is rotated to a detection wavelength position under the function of a control device, and detects a content of a target substance in a to-be-detected object in the reaction device under a wavelength. The detection method comprises the following steps: arranging the reaction device at the reaction position, and performing detection under the control of the control device. Through arrangement of the multi-wavelength light source device, which can be rotated to a proper detection wavelength position, the to-be-detected object can be detected with more accuracy and small error.
Description
Technical field
The invention belongs to technical field of biological, relate to a kind of pick-up unit and detection method thereof, be specifically related to a kind of fully-automatic multi-channel biochemical detection device and detection method thereof.
Background technology
POCT is the abbreviation of PointofCareTesting, can be translated as " scene is detected immediately ".POCT industry belongs to the segmented industry of IVD industry, is one of segmented industry that IVD industry development is the fastest in recent years.The application of POCT product is very extensive, divide from test item, mainly concentrate on 13 large projects such as the detection of the blood fat projects such as blood sugar test, vim and vigour and electrolyte analysis, fast Blood coagulation test, cardiac marker quick diagnosis, drug abuse screening, urinalysis, Dry-type biochemical detection, conceived test, fecal occult blood blood analysis, food pathogens examination, haemoglobin detection, infectious disease detection, triglyceride and cholesterol.
Biochemical, immunity in recent years, chemical detection process all require high efficiency, cost reduction and simple to operate.Therefore, it is desirable to come at a high speed with less automatic analysing apparatus and correctly detect project as much as possible.In this case, POCT Biochemical Analyzer is required the function possessing automatic identification, the at a high speed multiple test item of accurate process.
Summary of the invention
For solving the problems of the technologies described above, the object of the present invention is to provide a kind of fully-automatic multi-channel biochemical detection device and detection method thereof, thus can by multi-wavelength light source device, rotate to suitable detection wavelength location when detecting, thus thing to be detected is detected, that detects is more accurate, and error is little.
For achieving the above object, technical scheme of the present invention is as follows:
On the one hand, the invention provides a kind of fully-automatic multi-channel biochemical detection device, comprise base, also comprise multiple detecting unit and control device, described detecting unit is located on base, and described detecting unit comprises multi-wavelength light source device and reaction station, and described reaction station is used for placing response device, described multi-wavelength light source device turns to determined wavelength position under the effect of control device, and the target substance content of thing to be detected under described wavelength in detection reaction device.
Fully-automatic multi-channel biochemical detection device of the present invention, by arranging multi-wavelength light source device, can rotate to suitable detection wavelength location when detecting, thus to detect thing to be detected, detection more accurate, and error is little; And owing to arranging multiple detecting unit, multi wave length illuminating source detects multiple biochemical project respectively, and testing process is convenient and simple, the error that in prior art, troublesome operation exists can be reduced, multiple sample can be detected simultaneously, realize high flux and detect; Greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and biochemical analysis system volume of the present invention is little, structure is simple, highly sensitive, testing result is reliably accurate, can be widely used in the detection fields such as biological chemistry, immunity, chemistry; The Automated condtrol that overall process is realized except sample adds step by control device, realizes the calibration of Automated electronic curve, reactant liquor mixing, temperature of reaction control, timing and detection.
On the basis of technique scheme, the present invention also can make following improvement:
As preferred scheme, described multi-wavelength light source device comprises incident light source and multiple rotating optical filter, described multiple rotating optical filter is located at one end that described incident light source is connected with described base, described multiple rotating optical filter, under the effect of control device, makes the optical filter of determined wavelength turn in light path.
Adopt above-mentioned preferred scheme, the optical filter of multiple different wave length can, under the effect of control device, make the optical filter of suitable determined wavelength turn in light path, and detection speed is fast, and error is little.
As preferred scheme, described multiple rotating optical filter is connected with described base by swinging mounting.
As preferred scheme, described multi-wavelength light source device also comprises chlorine halogen tungsten lamp, optical fiber, reception light end.
As preferred scheme, also comprise transmitted light path device, described transmitted light path system comprises transmitted light path receiver, and described transmitted light path receiver is located on base, and described reaction station is located between described multiple rotating optical filter and described transmitted light receiver.
Adopt above-mentioned preferred scheme, reaction station is located between multiple rotating optical filter and transmitted light receiver, after optical filter turns to correct position, directly the light source of suitable determined wavelength is emitted to reaction station, read the information of reacted thing to be detected, then the information of reading is directly inputted into the reception of transmitted light receiver, save light source, lose little, the result of detection is accurate.
As preferred scheme, described multi-wavelength light source device also comprises incident light through hole, and one end of described incident light through hole is connected with described optical filter, and the other end of described incident light through hole is connected with described reaction station.
Adopt above-mentioned preferred scheme, the setting of incident light through hole can avoid the light between each detecting unit to disturb, and incident light directly enters reaction station by entrance pupil, reduce the impact on Detection results, thus testing result is more accurate.
As preferred scheme, described transmitted light path device also comprises transmitted light path channels or optical fiber, chrominance response cup and photoelectric conversion battery.
As preferred scheme, described reaction unit comprises the first reagent reacting cup and the second reagent reacting cup, first and second reagent reacting cup described is respectively used to hold first and second reagent, described second reagent reacting cup is inserted on the first reagent reacting cup, one end of described second reagent reacting cup is installed with irony spheroid, and the other end of described second reagent reacting cup is provided with the pressure ball parts be slidably connected with it; Described biochemical detection device also comprises push rod device and the stirring apparatus for driving irony spheroid to rotate, and described pressure ball parts fall into the first reagent reacting cup in the promotion of push rod device, makes first and second reagent and thing to be detected mixing.
Adopt above-mentioned preferred scheme, irony spheroid not only can play the effect of sealing second reagent reacting cup, can also play the effect of stirring, thus makes reaction result more accurate, and reaction velocity is faster.
As preferred scheme, described push rod device comprises the mechanical hook-up that can pump, and is linked at the thin inserted link on mechanical hook-up, moves downward for promoting pressure ball parts, makes first and second reagent and thing to be detected mixing.
As preferred scheme, described pressure ball parts are push-press type sealing-plug or push-press type enclosed switch.
Adopt above-mentioned preferred scheme, sealing-plug or enclosed switch can be selected as required.
As preferred scheme, described first reagent reacting cup becomes split type setting with the second reagent reacting cup, and described peristome is provided with peelable sealed membrane.
Adopt above-mentioned preferred scheme, first reagent reacting cup becomes split type setting with second component, separately sealing separately time no, what seal is better, connect reagent mixing wherein during use again, will, because of when sealing not offhand detection fortunately, make the reagent in the first reagent reacting cup and the second time parts enter the other side, reduce the amount detecting reagent or detect sample, and the data that impact detects when detecting.
As preferred scheme, described irony spheroid comprises iron core, and described iron core outer cladding is by elastomeric layer.
Adopt above-mentioned preferred scheme, the setting of iron core can be interrupted or magnetic field continuously effect under vertical tremor or horizontally rotate, thus make the first reagent, the second reagent and sample to be tested realize mixing fully, play good effect when stirring; Elastomeric layer makes irony spheroid have certain elasticity, can carry out corresponding deformation according to the change of the second reagent reacting cup, thus can make the second reagent reacting cup good seal.
As preferred scheme, described elastomeric layer is made up of rubber, silica gel or plastics.
As preferred scheme, described second reagent reacting cup is also arranged with Handheld Division outward.
Adopt above-mentioned preferred scheme, be convenient to pick and place, the second reagent reacting cup is installed.
As preferred scheme, described Handheld Division is provided with multiple projection.
Adopt above-mentioned preferred scheme, the setting of Handheld Division projection, anti-skidding effect can be played, safer when picking and placeing, not easily landing.
As preferred scheme, described Handheld Division is also provided with guide part, described guide part and described first reagent reacting cup suitable, and along described second reagent reacting cup is installed with irony spheroid one end extend.
Adopt above-mentioned preferred scheme, the setting of guide part, be convenient to the second reagent reacting cup to be arranged on the first reagent reacting cup, make both closely cooperate, the unlikely reactant liquor that makes spills.
As preferred scheme, when described second reagent reacting cup is inserted on described peristome, described peristome and the second reagent reacting cup entirety fall into the first reagent reacting cup, and described Handheld Division and described peristome are combined closely.
As preferred scheme, described Handheld Division is that cavity type is arranged, and one end of described Handheld Division is set in outside described pressure ball parts.
Adopt above-mentioned preferred scheme, save space, save material.
As preferred scheme, described biochemical detection device also comprises barcode recognition apparatus, and after described barcode recognition apparatus reads thing to be detected, control device is according to the information of the thing to be detected read, start the optical filter of determined wavelength in described multiple rotating optical filter, turn to light path position.
Adopt above-mentioned preferred scheme, arrange trace routine by barcode recognition apparatus, carry out the detection of respective items object respectively to the reactant liquor in different passage, testing process is simple, and Detection results is high.
As preferred scheme, described biochemical detection device also comprises heating arrangement, and described heating arrangement comprises electric boiling plate or heating wire below reaction station, is in the temperature of matters for the reactant liquor controlled in reaction unit.
As preferred scheme, described stirring apparatus comprises electromagnet, for driving the irony spheroid in reaction unit, the reactant liquor in reaction unit is mixed.
As preferred scheme, described fully-automatic multi-channel biochemical detection device also comprises housing, and described control device, multiple detecting unit and base are all arranged in described housing.
As preferred scheme, described housing arranges LCDs, described LCDs for showing actions menu, inquiry test result, thus operates more convenient.
On the other hand, the present invention also provides a kind of detection method of fully-automatic multi-channel biochemical detection device, comprises the following steps:
1) thing to be detected is added in the first reagent reacting cup, then the second reagent reacting cup is plugged in the first reagent reacting cup, obtain reaction unit, then described reaction unit is placed on reaction station;
2) after described control device senses the signal of reaction unit, the optical filter starting determined wavelength in multiple rotating optical filter turns in light path, it is descending that control device promotes pressure ball parts again, makes irony spheroid and the second reagent fall into the first reagent reacting cup;
3) then stirring apparatus restarted by control device, stirring apparatus drives irony spheroid to rotate, first and second reagent and thing to be detected mixing are obtained potpourri, reactant mixed liquor is obtained after described potpourri reaction, control device starts the concentration of transmitted light path device detection reaction potpourri, control device receives transmitted light path device again by the information of the concentration of the reaction mixture of detection, and the information of the concentration of the reaction mixture received is exported, take out reaction unit, then carry out the detection of next sample.
The detection method of fully-automatic multi-channel biochemical detection device of the present invention, can rotate to suitable detection wavelength location by arranging multi-wavelength light source device when detecting, thus to detect thing to be detected, detection more accurate, and error is little; And owing to detecting multiple biochemical project respectively for multi wave length illuminating source, testing process is convenient and simple, the error that in prior art, troublesome operation exists can be reduced, multiple sample can be detected simultaneously; Greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and biochemical analysis system volume of the present invention is little, structure is simple, highly sensitive, testing result is reliably accurate, can be widely used in the detection fields such as biological chemistry, immunity, chemistry; The Automated condtrol that overall process is realized except sample adds step by control device, realizes the calibration of Automated electronic curve, reactant liquor mixing, temperature of reaction control, timing and detection.
On the basis of technique scheme, the present invention also can make following improvement:
As preferred scheme, in step 2) in, control device to start in multiple rotating optical filter before the optical filter of determined wavelength turns in light path, also comprises and first starts barcode recognition apparatus, then is read the step of information of described thing to be detected by barcode recognition apparatus.
Adopt above-mentioned preferred scheme, arrange trace routine by barcode recognition apparatus, carry out the detection of respective items object respectively to the reactant liquor in different passage, testing process is simple, and Detection results is high.
As preferred scheme, in step 1) in, described thing to be detected comprises whole blood, serum and plasma, when described thing to be detected is whole blood, second reagent reacting cup is plugged in after in the first reagent reacting cup, after also comprising the steps: that control device senses the signal of reaction unit, the optical filter starting determined wavelength in multiple rotating optical filter turns in light path, described control device by calculating the content of haemoglobin, converses the amount of the actual serum added in the first reagent reacting cup again.
Adopt above-mentioned preferred scheme, detection method of the present invention not only can detect blood plasma and serum, whole blood can also be detected, do not need to carry out pre-separation to whole blood, after only need adding whole blood sample, allow the first reagent reacting in whole blood sample and the first reagent reacting cup, namely by calculating the content of haemoglobin, and the content of the actual serum contained in whole blood of being added by the content of haemoglobin, and then according to the serum content calculated, select corresponding trace routine, Detection results is high, and testing result is accurate.
Accompanying drawing explanation
Fig. 1 is the structural representation of the detecting unit in fully-automatic multi-channel biochemical detection device of the present invention;
Fig. 2 is the outside drawing of fully-automatic multi-channel biochemical detection device of the present invention;
Fig. 3 is the process flow diagram of the detection method of fully-automatic multi-channel biochemical detection device of the present invention;
Fig. 4 is the structural representation that the second reagent reacting cup of reaction unit in fully-automatic multi-channel biochemical detection device of the present invention is inserted on the first reagent reacting cup;
Fig. 5 is the decomposition texture schematic diagram of the reaction unit in fully-automatic multi-channel biochemical detection device of the present invention;
Fig. 6 is the structural representation of the irony spheroid of the reaction unit of fully-automatic multi-channel biochemical detection device of the present invention;
Wherein:
1. base, 2. detecting unit, 3. react station, 4. incident light source, 5. optical filter, 6. transmitted light path receiver, 7. incident light through hole, 8. transmitted light path channels, 9. the first reagent reacting cup, 10. the second reagent reacting cup, 101. Handheld Divisions, 102. guide parts, 103. is protruding, 11. irony spheroids, 1101. iron cores, 1102. elastomeric layers, 12. pressure ball parts, 13. peristomes, 14. housings, 1401, LCDs, 1402. reaction cabins, 15. stirring apparatuss.
Embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with accompanying drawing.
In order to reach object of the present invention, as shown in Fig. 1 to 2, fully-automatic multi-channel biochemical detection device of the present invention, comprise base 1, also comprise multiple detecting unit 2 and control device, detecting unit 2 is located on base 1, detecting unit 2 comprises multi-wavelength light source device and reaction station 3, reaction station 3 is for placing response device, and multi-wavelength light source device turns to determined wavelength position under the effect of control device, and the target substance content of thing to be detected under described wavelength in detection reaction device.Fully-automatic multi-channel biochemical detection device of the present invention, can rotate to suitable wavelength location by arranging multi-wavelength light source device when detecting, thus to detect thing to be detected, detection more accurate, and error is little; And owing to arranging multiple detecting unit, multi wave length illuminating source detects multiple biochemical project respectively, and testing process is convenient and simple, the error that in prior art, troublesome operation exists can be reduced, multiple sample can be detected simultaneously, realize high flux and detect; Greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and biochemical analysis system volume of the present invention is little, structure is simple, highly sensitive, testing result is reliably accurate, can be widely used in the detection fields such as biological chemistry, immunity, chemistry; The Automated condtrol that overall process is realized except sample adds step by control device, realizes the calibration of Automated electronic curve, reactant liquor mixing, temperature of reaction control, timing and detection.
In order to optimize implementation result of the present invention further, multi-wavelength light source device comprises incident light source 4 and multiple rotating optical filter 5, multiple rotating optical filter 5 is located at one end that described incident light source 4 is connected with base 1, multiple rotating optical filter 5 can, under the effect of control device, make the optical filter of determined wavelength turn in light path.The optical filter of multiple different wave length can, under the effect of control device, make the optical filter of determined wavelength turn in light path, and detection speed is fast, and error is little.
In order to optimize implementation result of the present invention further, multiple rotating optical filter 5 is connected with base 1 by swinging mounting.
In order to optimize implementation result of the present invention further, multi-wavelength light source device also comprises chlorine halogen tungsten lamp, optical fiber, reception light end.
In order to optimize implementation result of the present invention further, also comprise transmitted light path device, described transmitted light path system comprises transmitted light path receiver 6, and transmitted light path receiver 6 is located on base 1, and reacts station 3 and be located between multiple rotating optical filter 5 and transmitted light receiver 6.Reaction station is located between multiple rotating optical filter and transmitted light receiver, optical filter turns to detecting position postpone, directly the light source of determined wavelength is emitted to reaction station, read the information of reacted thing to be detected, again the information of reading is directly inputted into transmitted light receiver to receive, save light source, lose little, the result of detection is accurate.
In order to optimize implementation result of the present invention further, multi-wavelength light source device also comprises incident light through hole 7, and one end of incident light through hole 7 is connected with optical filter 5, and the other end of incident light through hole 7 is connected with reaction station 3.The setting of incident light through hole can avoid the light between each detecting unit to disturb, and incident light directly enters reaction station by entrance pupil, reduce the impact on Detection results, thus testing result is more accurate.
In order to optimize implementation result of the present invention further, transmitted light path device also comprises transmitted light path channels 8 or optical fiber, chrominance response cup and photoelectric conversion battery, one end of transmitted light path channels 8 is connected with reaction station 3, and the other end is connected with transmitted light path receiver 6.
In order to optimize implementation result of the present invention further, as shown in Fig. 4 to 5, reaction unit comprises the first reagent reacting cup 9 and the second reagent reacting cup 10, first and second reagent reacting cup 9,10 is respectively used to hold first and second reagent, second reagent reacting cup 10 is inserted on the first reagent reacting cup 9, the other end that one end of second reagent reacting cup 10 is installed with irony spheroid 11, second reagent reacting cup 10 is provided with the pressure ball parts 12 be slidably connected with it; Biochemical detection device also comprises push rod device and the stirring apparatus 15 for driving irony spheroid 11 to rotate, and pressure ball parts 12 fall into the first reagent reacting cup 9 in the promotion of push rod device, makes first and second reagent and thing to be detected mixing.Irony spheroid not only can play the effect of sealing second reagent reacting cup, can also play the effect of stirring, thus makes reaction result more accurate, and reaction velocity is faster.
In order to optimize implementation result of the present invention further, push rod device comprises the mechanical hook-up that can pump, and is linked at the thin inserted link on mechanical hook-up, moves downward for promoting pressure ball parts 12, makes first and second reagent and thing to be detected mixing.
In order to optimize implementation result of the present invention further, pressure ball parts 12 are push-press type sealing-plug or push-press type enclosed switch.Sealing-plug or enclosed switch can be selected as required.
In order to optimize implementation result of the present invention further, the setting split type with the second reagent reacting cup 10 one-tenth of first reagent reacting cup 9, first reagent reacting cup 9 is provided with peristome 13, peristome 13 is provided with peelable sealed membrane, and the second reagent reacting cup 10 is inserted on the first reagent reacting cup 9 by peristome 13.First reagent reacting cup becomes split type setting with second component, separately sealing separately time no, what seal is better, connect reagent mixing wherein during use again, can not because of when sealing not offhand detection fortunately, make the reagent in the first reagent reacting cup and the second time parts enter the other side, reduce the amount detecting reagent or detect sample, and the data that impact detects when detecting.
In order to optimize implementation result of the present invention further, as shown in Figure 6, irony spheroid 11 comprises iron core 1101, and iron core 1101 outer cladding is by elastomeric layer 1102.The setting of iron core can be interrupted or magnetic field continuously effect under vertical tremor or horizontally rotate, thus make the first reagent, the second reagent and sample to be tested realize mixing fully, play good effect when stirring; Elastomeric layer makes irony spheroid have certain elasticity, can carry out corresponding deformation according to the change of the second reagent reacting cup, thus can make the second reagent reacting cup good seal.
In order to optimize implementation result of the present invention further, elastomeric layer is made up of rubber, silica gel or plastics.
In order to optimize implementation result of the present invention further, the second reagent reacting cup 10 is outer is also arranged with Handheld Division 101.Be convenient to pick and place, the second reagent reacting cup is installed.
In order to optimize implementation result of the present invention further, Handheld Division 101 is provided with multiple protruding 103.The setting of Handheld Division projection, can play anti-skidding effect, safer when picking and placeing, not easily landing.
In order to optimize implementation result of the present invention further, Handheld Division 101 is also provided with guide part 102, guide part 102 and the first reagent reacting cup 9 suitable, and along the second reagent reacting cup 10 is installed with irony spheroid 11 one end extend.The setting of guide part, is convenient to the second reagent reacting cup to be arranged on the first reagent reacting cup, and make both closely cooperate, the unlikely reactant liquor that makes spills.
In order to optimize implementation result of the present invention further, when the second reagent reacting cup 10 is inserted on peristome 13, peristome 13 and the second reagent reacting cup 10 entirety fall into the first reagent reacting cup 9, and Handheld Division 101 and peristome 13 are combined closely.
In order to optimize implementation result of the present invention further, Handheld Division 101 is cavity type setting, and one end of Handheld Division 101 is set in outside pressure ball parts 12.Save space, save material.
In order to optimize implementation result of the present invention further, biochemical detection device also comprises barcode recognition apparatus, after barcode recognition apparatus reads thing to be detected, control device, according to the information of the thing to be detected read, starts multiple rotating optical filter 5 and turns in light path.Arrange trace routine by barcode recognition apparatus, carry out the detection of respective items object respectively to the reactant liquor in different passage, testing process is simple, and Detection results is high.
In order to optimize implementation result of the present invention further, biochemical detection device also comprises heating arrangement, and heating arrangement comprises electric boiling plate or heating wire below reaction station, is in the temperature of matters for the reactant liquor controlled in reaction unit.
In order to optimize implementation result of the present invention further, stirring apparatus 15 comprises electromagnet, for driving the irony spheroid 11 in reaction unit, the reactant liquor in reaction unit is mixed.
In order to optimize implementation result of the present invention further, fully-automatic multi-channel biochemical detection device also comprises housing 14, and control device, multiple detecting unit and base 1 are all arranged in housing 14.
In order to optimize implementation result of the present invention further, housing 14 is arranged LCDs 1401 and reaction cabin 1402, LCDs 1401 is for showing actions menu, inquiry test result.
In order to reach object of the present invention, as shown in Figure 3, the detection method of fully-automatic multi-channel biochemical detection device of the present invention, comprises the following steps:
1) thing to be detected is added in the first reagent reacting cup 9, then the second reagent reacting cup 10 is plugged in the first reagent reacting cup 9, obtain reaction unit, then described reaction unit is placed on reaction station;
2) after described control device senses the signal of reaction unit, the optical filter 5 starting determined wavelength in multiple rotating optical filter turns in light path, it is descending that control device promotes pressure ball parts 12 again, makes irony spheroid 11 and the second reagent fall into the first reagent reacting cup;
3) then stirring apparatus 15 restarted by control device, stirring apparatus 15 drives irony spheroid 11 to rotate, first and second reagent and thing to be detected mixing are obtained potpourri, reactant mixed liquor is obtained after potpourri reaction, control device starts the concentration of transmitted light path device detection reaction potpourri, control device receives transmitted light path device again by the information of the concentration of the reaction mixture of detection, and the information of the concentration of the reaction mixture received is exported, take out reaction unit, carry out the detection of next sample again.
The detection method of fully-automatic multi-channel biochemical detection device of the present invention, can rotate to determined wavelength position by arranging multi-wavelength light source device when detecting, thus to detect thing to be detected, detection more accurate, and error is little; And owing to detecting multiple biochemical project respectively for multi wave length illuminating source, testing process is convenient and simple, the error that in prior art, troublesome operation exists can be reduced, multiple sample can be detected simultaneously; Greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and biochemical analysis system volume of the present invention is little, structure is simple, highly sensitive, testing result is reliably accurate, can be widely used in the detection fields such as biological chemistry, immunity, chemistry; The Automated condtrol that overall process is realized except sample adds step by control device, realizes the calibration of Automated electronic curve, reactant liquor mixing, temperature of reaction control, timing and detection.
In order to optimize implementation result of the present invention further, in step 2) in, before in the multiple rotating optical filter of control device startup, the optical filter of determined wavelength turns in light path, also comprise and first start barcode recognition apparatus, then read the step of information of described thing to be detected by barcode recognition apparatus.Arrange trace routine by barcode recognition apparatus, carry out the detection of respective items object respectively to the reactant liquor in different passage, testing process is simple, and Detection results is high.
In order to optimize implementation result of the present invention further, in step 1) in, described thing to be detected comprises whole blood, serum and plasma, when described thing to be detected is whole blood, second reagent reacting cup is plugged in after in the first reagent reacting cup, after also comprising the steps: that control device senses the signal of reaction unit, the optical filter starting determined wavelength in multiple rotating optical filter turns in light path, described control device by calculating the content of haemoglobin, converses the amount of the actual serum added in the first reagent reacting cup again.Detection method of the present invention not only can detect blood plasma and serum, whole blood can also be detected, do not need to carry out pre-separation to whole blood, after only need adding whole blood sample, allow the first reagent reacting in whole blood sample and the first reagent reacting cup, namely by calculating the content of haemoglobin, and the content of the actual serum contained in whole blood of being added by the content of haemoglobin, and then according to the serum content calculated, select corresponding trace routine, Detection results is high, and testing result is accurate.
Below in the mode of specific embodiment, the step detecting whole blood is described:
Step one, start preheating, makes system reach working temperature;
Step 2, blinking red lamp in LCDs 1401 sash of reaction cabin 1402 correspondence, shows that this reaction station is ready, can detect;
Step 3, takes out the first reagent reacting cup 9, tears sealed membrane off, draws x microliters of sample add in the first reagent reacting cup 9 with kapillary.Again the peristome 13 of the second reagent reacting cup 10 lower end and the first reagent reacting cup 9 upper end is fitted together forming reactions device. reaction unit is placed on the reaction station in reaction cabin 1402, detector tube inductor by Signal transmissions to control device, green light flicker in LCDs 1401 sash of reaction cabin 1402 correspondence, start filter supporter to rotate simultaneously, green color filter is turned in light path.The change (reddening) of transmitted light path receiver 6 Quorum Sensing liquid color, start reaction timer, control device converts the content of haemoglobin automatically, and converts packed cell volume to, converses the actual amount adding serum in reaction cup.
Step 4, control device starts Quick Response Code (bar code) recognition device, according to the information that Quick Response Code in reaction cup (bar code) comprises, starts filter supporter and rotates, turned in light path by the optical filter 5 of corresponding wavelength.
Step 5, control device starts lower plunger thrust unit, is pushed downwards by the irony spheroid 11 in the second reagent reacting cup 10 in first reagent reacting cup 9, and the second reagent flows in the first reagent reacting cup 9 simultaneously.
Step 6, control device starts stirring apparatus 15, and stirring apparatus 15 stopped afterwards automatically at 5 ~ 10 seconds, and the green light in corresponding LCDs 1401 sash stops flicker, the concentration of reactant liquor in transmitted light path device start detection reaction unit.
Step 7, after reaction n second, in LCDs 1401 sash, green light reddens lamp, and optical system stops measuring, and in LCDs 1401, show the testing result of biochemical indicator.
Step 7, takes out and detects complete reaction cup 5, and now in LCDs 1401 sash, red light starts flicker again, and expression can carry out next sample tests.
The present invention compared with prior art has significant progressive and advantage, be applicable to the biochemistry of double reagent, immunity, chemical reaction liquid is tested, the transmitted light system arranging different wave length can detect multiple test item respectively, the Automated condtrol that overall process is realized except sample adds step by control device, testing process is convenient and simple, the error that in existing POCT technology, troublesome operation exists can be reduced, biochemical detection device of the present invention can detect several different samples simultaneously in addition, greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and specific protein biochemical detection device volume of the present invention is little, structure is simple, highly sensitive, testing result is reliably accurate, various biochemistry can be widely used for, immunity, the analysis and resolution of chemical reaction.
Above-described is only the preferred embodiment of the present invention, it should be pointed out that for the person of ordinary skill of the art, and without departing from the concept of the premise of the invention, can also make some distortion and improvement, these all belong to protection scope of the present invention.
Claims (10)
1. a fully-automatic multi-channel biochemical detection device, comprise base, it is characterized in that, also comprise multiple detecting unit and control device, described detecting unit is located on base, and described detecting unit comprises multi-wavelength light source device and reaction station, and described reaction station is used for placing response device, described multi-wavelength light source device turns to determined wavelength position under the effect of control device, and the target substance content of thing to be detected under described wavelength in detection reaction device.
2. fully-automatic multi-channel biochemical detection device according to claim 1, it is characterized in that, described multi-wavelength light source device comprises incident light source and multiple rotating optical filter, described multiple rotating optical filter is located at one end that described incident light source is connected with described base, described multiple rotating optical filter, under the effect of control device, makes the optical filter of determined wavelength turn in light path.
3. fully-automatic multi-channel biochemical detection device according to claim 2, is characterized in that, described multiple rotating optical filter is connected with described base by swinging mounting.
4. fully-automatic multi-channel biochemical detection device according to any one of claim 1 to 3, it is characterized in that, described biochemical detection device also comprises transmitted light path device, described transmitted light path system comprises transmitted light path receiver, described transmitted light path receiver is located on base, and described reaction station is located between described multiple rotating optical filter and described transmitted light receiver.
5. fully-automatic multi-channel biochemical detection device according to claim 4, it is characterized in that, described multi-wavelength light source device also comprises incident light through hole, one end of described incident light through hole is connected with described multiple rotating optical filter, and the other end of described incident light through hole is connected with described reaction station.
6. fully-automatic multi-channel biochemical detection device according to any one of claim 1 to 5, it is characterized in that, described reaction unit comprises the first reagent reacting cup and the second reagent reacting cup, first and second reagent reacting cup described is respectively used to hold first and second reagent, described second reagent reacting cup is inserted on the first reagent reacting cup, one end of described second reagent reacting cup is installed with irony spheroid, and the other end of described second reagent reacting cup is provided with the pressure ball parts be slidably connected with it; Described biochemical detection device also comprises push rod device and the stirring apparatus for driving irony spheroid to rotate, and described pressure ball parts fall into the first reagent reacting cup in the promotion of push rod device, makes first and second reagent and thing to be detected mixing.
7. fully-automatic multi-channel biochemical detection device according to any one of claim 1 to 6, it is characterized in that, described biochemical detection device also comprises barcode recognition apparatus, after described barcode recognition apparatus reads thing to be detected, control device is according to the information of the thing to be detected read, start the optical filter of determined wavelength in described multiple rotating optical filter, turn to light path position.
8. the detection method of fully-automatic multi-channel biochemical detection device according to any one of claim 1 to 7, comprises the following steps:
1) thing to be detected is added in the first reagent reacting cup, then the second reagent reacting cup is plugged in the first reagent reacting cup, obtain reaction unit, then described reaction unit is placed on reaction station;
2) after described control device senses the signal of reaction unit, the optical filter starting determined wavelength in multiple rotating optical filter turns in light path, it is descending that control device promotes pressure ball parts again, makes irony spheroid and the second reagent fall into the first reagent reacting cup;
3) then stirring apparatus restarted by control device, stirring apparatus drives irony spheroid to rotate, first and second reagent and thing to be detected mixing are obtained potpourri, reactant mixed liquor is obtained after described potpourri reaction, control device starts the concentration of transmitted light path device detection reaction potpourri, control device receives transmitted light path device again by the information of the concentration of the reaction mixture of detection, and the information of the concentration of the reaction mixture received is exported, take out reaction unit, then carry out the detection of next sample.
9. the detection method of fully-automatic multi-channel biochemical detection device according to claim 8, it is characterized in that, in step 2) in, before in the multiple rotating optical filter of control device startup, the optical filter of determined wavelength turns in light path, also comprise and first start barcode recognition apparatus, then read the step of information of described thing to be detected by barcode recognition apparatus.
10. the detection method of fully-automatic multi-channel biochemical detection device according to claim 8 or claim 9, is characterized in that, in step 1) in, described thing to be detected comprises whole blood, serum and plasma; Preferably, when described thing to be detected is whole blood, second reagent reacting cup is plugged in after in the first reagent reacting cup, after also comprising the steps: that control device senses the signal of reaction unit, the optical filter starting determined wavelength in multiple rotating optical filter turns to light path position, described control device by calculating the content of haemoglobin, converses the amount of the actual serum added in the first reagent reacting cup again.
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