CN105541831B - Medicine root berberrubine derived from amur cork-tree processed product and separation preparation method and application thereof - Google Patents

Medicine root berberrubine derived from amur cork-tree processed product and separation preparation method and application thereof Download PDF

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CN105541831B
CN105541831B CN201610026002.7A CN201610026002A CN105541831B CN 105541831 B CN105541831 B CN 105541831B CN 201610026002 A CN201610026002 A CN 201610026002A CN 105541831 B CN105541831 B CN 105541831B
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medicine
medicine root
cortex phellodendri
root
berberrubine
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CN105541831A (en
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张凡
刘蓬蓬
贾天柱
徐珊
高慧
史辑
鞠成国
林桂梅
单国顺
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Liaoning University of Traditional Chinese Medicine
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D455/00—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine
    • C07D455/03—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine containing quinolizine ring systems directly condensed with at least one six-membered carbocyclic ring, e.g. protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine

Abstract

The invention provides medicine root berberrubine derived from an amur cork-tree processed product and a separation preparation method and application thereof. The method includes the steps that 1, jatrorrhizine hydrochloride reference substances are precisely weighed, placed in an evaporation dish to be baked, taken out and cooled, chromatographic grade methyl alcohol is added, millipore filter filtering is conducted, and a baked jatrorrhizine reference substance solution is obtained; 2, the baked jatrorrhizine reference substance solution is separated through a preparative liquid phase, eluant of a target chromatographic peak is collected and purified through a sephadex column, the purified eluant is subjected to nitrogen blowing and freeze drying, and pink powder, namely, medicine root berberrubine crystals prepared through separation are obtained. The prepared medicine root berberrubine is a new compound found in amur cork-tree processed decoction pieces, the separation preparation method has the advantages of being high in efficiency, stable in process, easy and convenient to operate and low in cost, high-purity separation preparation of a large number of medicine root berberrubine monomers can be achieved, and the medicine root berberrubine compound has good macrophage phagocytosis and high immunization.

Description

A kind of red alkali of medicine root and its method for separating and preparing and purposes for coming from Cortex Phellodendri processed product
Technical field
The present invention relates to technical field of Chinese medicine, more particularly to a kind of red alkali of medicine root for coming from Cortex Phellodendri processed product and its separation Preparation method and purposes.
Background technology
Cortex Phellodendri begins to be loaded in《Sheng Nong's herbal classic》, it is rutaceae wampeePhellodendron chinense Or Cortex Phellodendri Schneid.PhellodendronThe dry bark of amurense Rupr., existing more than 2,000 years medicinal history, For traditional Chinese medical science tradition heat clearing and fire purging medicine.Ancient Chinese medicine doctor attaches great importance to the processing to Cortex Phellodendri in clinical practice, and its processed product is shown in earliest Yu Jindai, Ge Hong's《Zhouhou Beiji Fang》.Traditional concocting method of Cortex Phellodendri has net system, cutting, processed with honey, processed with wine, salt system, charcoal 16 kinds of concocting methods such as system, milk system, urine of baby boys are now more based on processed with wine, salt system and charcoal processing.
The composition of alkaloids such as berberine, palmatine, jateorhizine, phellodendrine are mainly contained in Cortex Phellodendri.Modern pharmacology is acted on Research shows that there is Cortex Phellodendri blood sugar lowering, blood pressure lowering, anticancer, antioxidation etc. to act on.Cortex Phellodendri is in concocting process due to the impact of temperature Its chemical composition can occur certain change, and because adding different adjuvants after, its property of medicine also can be varied from, and ultimately result in medicine The difference of reason effect.The high-efficient liquid phase chromatogram discovery that product, salt process product is processed by comparing Cortex Phellodendri health product, wine, Cortex Phellodendri is Jing after processing There is new chromatographic peak to generate.This laboratory early-stage Study result shows that Cortex Phellodendri has two chemical compositions in concocting process Change, one of them is the berberrubine being transformed by berberine, another is the Ba Mahong being transformed by palmatine Spit of fland, at the same it was observed that the content of jateorhizine decreases after processing, therefore heat treated has been carried out to jateorhizine monomer, find A newly-generated chromatographic peak on high-efficient liquid phase chromatogram after one newly-generated chromatographic peak and the peak and Cortex Phellodendri processing has phase Same retention time.Thus, thus it is speculated that in Cortex Phellodendri processed product the newly-generated chemical composition be in Cortex Phellodendri jateorhizine in concocting process In be transformed.Binding experiment room early-stage Study result, is that the alkaloid before and after whole observation Cortex Phellodendri processes changes and former little Changing Pattern of the bark of a cork tree alkaline alkaloid in concocting process, therefore to jateorhizine（C20H20NO4）Processing before and after chemical conversion New component is prepared separation.
The content of the invention
For the problems referred to above, the present invention provide a kind of red alkali of medicine root and its method for separating and preparing for coming from Cortex Phellodendri processed product and Purposes, the method prepares the red alkali of medicine root from separation in Cortex Phellodendri processed product, with separation efficiency height, process stabilizing, it is easy to operate, into This is cheap, is capable of achieving the high-load high-purity of the red alkali monomer of a large amount of medicine roots（More than 98%）Separate and prepare.
To realize the above-mentioned purpose of the present invention, the present invention provides a kind of red alkali of medicine root for coming from Cortex Phellodendri processed product, molecular formula For C19H17NO4, the red alkali of medicine root is named as, chemical structural formula is as follows.
The present invention also provides a kind of method for separating and preparing of the red alkali of medicine root for coming from Cortex Phellodendri processed product, comprises the following steps.
Step 1, the simulation of jateorhizine are processed：Precision weighs Jatrorrhizine chloride reference substance, in putting evaporating dish, 160~216 15~25min is baked at DEG C, taking-up is cooled, and adds hplc grade methanol, then microporous filter membrane（0.45μm）Filter, after must baking Jateorhizine reference substance solution.
Step 2, the separation of jateorhizine monomer baking product new component and preparation.
Preparative liquid chromatography condition：Mobile phase A is water（Wherein 100ml water diethylamine containing 0.4ml）, B is methanol；Mobile phase Flow velocity：The mobile phase ratio of A and B is（70~90:30~10, volume ratio）；With anti-phase C18Bonded silica stationary phase is chromatographic column Filler；25 DEG C of column temperature；Ultraviolet detection wavelength is 220~345nm；Sample size is 100 μ L.
Jateorhizine reference substance solution after baking is separated with preparation liquid phase, the eluent of target chromatographic peak is collected simultaneously Jing polydextran gel column purifications；The eluent that purification is crossed carries out nitrogen and blows 15min, and lyophilization at putting -80 DEG C is to be obtained pink Color powder, the red alkali crystallization of medicine root of as separated preparation.
The red alkali of the medicine root is used to prepare immunoregulatory medicine.
Beneficial effects of the present invention compared with prior art.
What the present invention was provided comes from the red alkali of medicine root and its method for separating and preparing and purposes of Cortex Phellodendri processed product, prepared medicine The red alkali of root is the noval chemical compound found in Cortex Phellodendri processes decoction pieces, and the preparative separation method has efficiency high, process stabilizing, operation Easy, with low cost the characteristics of, it is capable of achieving the high-purity of the red alkali monomer of a large amount of medicine roots（More than 98%）Separate and prepare, and the medicine root Red alkali cpd has the phagocytosiss of good macrophage, there is stronger immunization, relative to original life in Cortex Phellodendri Alkaloids have higher immune effect.
Description of the drawings
Fig. 1 is the preparative liquid chromatography figure of the red alkali of medicine root, and wherein A is the red alkali of medicine root.
Fig. 2 is the red alkali of medicine root1H-NMR is composed.
Fig. 3 is the red alkali of medicine root13C-NMR is composed.
Fig. 4 is the relative phagocytic rate of variable concentrations jateorhizine and the red alkali of medicine root to cell.
Specific embodiment
The present invention is further described with reference to specific embodiment, but this should not be interpreted as above-mentioned theme of the invention Scope be only limitted to following embodiments, all technologies realized based on the above of the present invention belong to the scope of the present invention.
Embodiment 1.
1st, instrument and material.
1.1 instrument：Semi-preparative liquid chromatography instrument（Shimadzu LC-10AT）；The a ten thousandth of METTLER AE240 types ten analyzes day It is flat（Switzerland METTLER）；FA1004B type electronic balances（Shanghai Precision Scientific Apparatus Co., Ltd）；YP5102 type electronic balances （The positive Medical Instruments company limited of upper sea light）；KQ-250DB type numerical control ultrasonic cleaners（The limited public affairs of city of Kunshan's ultrasonic instrument Department）；Nitrogen evaporator（Tianjin Hengao Technology Development Co., Ltd.）；101 type electric drying oven with forced convections（Beijing's bright medical instrument forever Device factory）；CHRIST vacuum freeze driers（Germany）；The semi-automatic point sample instrument of CAMAG Linomat-5 types（Switzerland）.
1.2 reagent：Cortex Phellodendri medical material is purchased from medical material company of Sichuan Province；Jatrorrhizine chloride reference substance is purchased from Sichuan Province Wei Keqisheng Thing Science and Technology Ltd. (lot number 130218)；The red alkali of medicine root is laboratory self-control, Jing high effective liquid chromatography for measuring, purity ＞ 98%；Thin layer chromatography silica gel plate（Haiyang Chemical Plant, Qingdao）；Sephadex LH-20 ( Pharmacia)；Methanol analysis is pure（My god The big luxuriant chemical reagent factory in Jinshi City）；Acetonitrile, methanol chromatographically pure；Diethyl amine analysis are pure（Tianjin great Mao chemical reagent factories）；Water is pure Water purification.
2nd, the present embodiment provides a kind of method for separating and preparing of the red alkali of medicine root for coming from Cortex Phellodendri processed product, including following step Suddenly.
Step 1, by Cortex Phellodendri index composition jateorhizine reference substance be simulated process：Precision weighs Jatrorrhizine chloride Reference substance 5mg, in putting evaporating dish, bakes 20min at 216 DEG C, and taking-up is cooled, and adds hplc grade methanol 5mL, then micropore filter Film（0.45μm）Filter, the jateorhizine reference substance solution after must baking carries out the preparation of new component.
Step 2, the separation of jateorhizine monomer baking product new component and preparation, refer to Fig. 1.
Preparative liquid chromatography condition：Mobile phase A is water, wherein 100ml water diethylamine containing 0.4ml, and B is methanol；Mobile phase Flow velocity：The mobile phase ratio of A and B is（80:20）；With anti-phase C18Bonded silica stationary phase is chromatographic column filler；25 DEG C of column temperature；It is purple Outer Detection wavelength is 345nm；Sample size is 100 μ L.
Jateorhizine reference substance solution after baking is separated with preparation liquid phase, the eluent of target chromatographic peak is collected simultaneously Jing polydextran gel column purifications；The eluent that purification is crossed carries out nitrogen and blows 15min, and lyophilization at putting -80 DEG C is to be obtained pink Color powder, the red alkali crystallization of medicine root of as separated preparation.
3rd, the identification for separating the red alkali cpd of medicine root for preparing, refers to Fig. 2 and Fig. 3.
Isolated composition, Jing1H-NMR、13C-NMR Analysis and Identification.1H-NMR (MeOD, 300 MHz) δ:δH:7.53 (1H, s, H-1), 6.80 (1H, s, H-4), 3.12 (2H, t, H-5), 4.65 (2H, t, H-6), 9.34 (1H, s, H-8), 7.62 (1H, d, H-11), 7.01 (1H, d, H-12), 8.22 (1H, s, H-13), 3.93 (3H, s, 2-OCH3), 3.99 (3H, s, 2- OCH3)；13C-NMR (MeOD, 75 MHz) δ:δC:109.5 (C-1), 149.4 (C-2), 150.6 (C-3), 115.9 (C-4), 28.4 (C-5), 56.0 (C-6), 147.3 (C-8), 124.9 (C-8a), 169.3 (C-9), 57.0 (10-OCH3), 129.9 (C- 11), 129.5 (C-12), 134.3 (C-12a), 120.6 (C-13), 136.6 (C-14), 119.3 (C-14a), according to above number Gained and outward appearance pinkiness are converted according to the structure that new component is determined with document and by jateorhizine, therefore the new component is named as into medicine The red alkali of root, molecular formula is C19H17NO4.The jateorhizine path for transformation is as follows.
4th, it is further checking beneficial effects of the present invention, there is provided below " Activity determination of the red alkali of medicine root stimulates Jing LPS Macrophage immunoregulation effect " test case.
4.1 assay method.
The RAW264.7 cells of exponential phase adjust cell density to 1 × 10 Jing after digestion, scraping, piping and druming7Individual/ ML, and be inoculated on 96 well culture plates, per the μ L of pore volume 100.37 DEG C are put, 5% CO2Incubator is incubated after 24 h, abandons supernatant. It is divided into 3 groups, i.e. blank control group：Add the blank μ L of culture fluid 200；Model control group：The μ L of LPS 100 of 2 μ g/mL are added, And final volume is diluted to as 200 μ L with blank culture fluid；Administration group：7 variable concentrations are added containing jateorhizine and the red alkali of medicine root The μ L of LPS 100 of the μ L of dosing culture fluid 100 and 2 μ g/mL（The final concentration of 1 μ g/mL of LPS）Final volume be 200 μ L, each concentration If 3 multiple holes.37 DEG C, 5% CO2After 24 h are cultivated in incubator, after abandoning culture supernatant, 0.1% neutral red solution 200 is added μ L, 37 DEG C, 5% CO260 min are incubated, dimethyl diaminophenazine chloride is abandoned, 3 times is washed with the PBS of pre-temperature, every time 200 μ L, and are dried.Addition acetic acid- Ethanol（1:1, volume ratio）The μ L/ holes of cytolysate 200,4 DEG C stand overnight, and OD values are surveyed at the nm of microplate reader 570.And calculate thin Born of the same parents are with respect to phagocytic rate（RSR）.
Cell with respect to phagocytic rate (%)=（OD experimental group-OD blank groups）/（OD matched group-OD blank groups）×100%.
Statistical procedures are carried out using the softwares of SPSS 19.0, measurement data is represented with " means standard deviation ", examined using t Testing carries out comparing between group, and P ＜ 0.05 are variant, with statistical significance.
4.2 measurement results, refer to table 1 and Fig. 4.
Table 1：The impact of jateorhizine and the red alkali of medicine root to RAW264.7 macrophage phagocytics.
Group OD570 With respect to phagocytic rate (%)
Blank group 0.192±0.004 —
Matched group 0.324±0.004 —
100 μ g/mL jateorhizine 0.316±0.008 93.94
The red alkali of 100 μ g/mL medicine roots 0.506±0.048** 237.88
50 μ g/mL jateorhizine 0.368±0.015 133.33
The red alkali of 50 μ g/mL medicine roots 0.596±0.059** 306.06
25 μ g/mL jateorhizine 0.474±0.064 213.64
The red alkali of 25 μ g/mL medicine roots 0.628±0.045** 330.30
10 μ g/mL jateorhizine 0.566±0.013 283.33
The red alkali of 10 μ g/mL medicine roots 0.709±0.075* 391.67
5 μ g/mL jateorhizine 0.590±0.022 301.52
The red alkali of 5 μ g/mL medicine roots 0.736±0.088** 412.12
2.5 μ g/mL jateorhizine 0.473±0.015 212.88
The red alkali of 2.5 μ g/mL medicine roots 0.635±0.062* 335.61
1 μ g/mL jateorhizine 0.293±0.096 76.52
The red alkali of 1 μ g/mL medicine roots 0.420±0.007** 172.73
Compare * with jateorhizineP＜ 0.05, * *P＜ 0.01.
As a result show, the red alkali of medicine root has stronger facilitation compared with jateorhizine to macrophage phagocytic effect, illustrates medicine The red alkali of root compared with jateorhizine be relatively beneficial to phagocyte inflammation phase remove body injury at tissue and cell Necrotic Debris with And pathogen and affect the process of wound healing.
5th, the assay of jateorhizine, the red alkali of medicine root in Cortex Phellodendri, phellodendron bark, Cortex Phellodendri (processed with wine), stir-baked CORTEX PHELLODENDRI with salt solution is fried.
The preparation of the red alkali reference substance solution of 5.1 jateorhizine, medicine root.
Precision weighs the mg of jateorhizine 2.06, in putting 50mL volumetric flasks, dissolves and be diluted to scale, and making concentration is The reference substance solution of 0.0412 mg/mL.
Precision weighs the new component isolated, and the red alkali 0.72mg of medicine root in putting 10mL volumetric flasks, dissolves and be diluted to quarter Degree, makes the reference substance solution that concentration is 0.0720mg/mL.
5.2 the preparation of test sample.
Fry Cortex Phellodendri and take net Cortex Phellodendri decoction pieces 50g, 6min is fried in pot, take out, crush, cross 60 mesh sieves.
Phellodendron bark takes net Cortex Phellodendri decoction pieces 50g, crushes, and crosses 60 mesh sieves.
Cortex Phellodendri (processed with wine) takes net Cortex Phellodendri decoction pieces 50g, adds yellow wine（The water of the wine of 20% decoction pieces amount, 15% decoction pieces amount）Mix thoroughly, moistening 12h, puts parch 6min in 150~160 DEG C of iron pans, takes out, and cools, and crushes, and crosses 60 mesh sieves.
Stir-baked CORTEX PHELLODENDRI with salt solution takes net Cortex Phellodendri decoction pieces 50g, adds saline solution（The water of the salt of 2% decoction pieces amount, 25% decoction pieces amount, cools）, mix Even, moistening puts parch 6min in 150~160 DEG C of iron pans, takes out, and cools, and crushes, and crosses 60 mesh sieves.
Take and fry Cortex Phellodendri, phellodendron bark, Cortex Phellodendri (processed with wine), stir-baked CORTEX PHELLODENDRI with salt solution powder about 0.5g, it is accurately weighed, in putting conical flask with cover, claim Determine weight, the min of supersound process 60 takes out, lets cool, and with extraction solution the weight of reduction is supplied, and shakes up, and stands overnight, and filters, Subsequent filtrate is taken, is obtained final product.
5.3 experimental result.
Each need testing solution is taken respectively appropriate, be measured according to following chromatographic condition, the results are shown in Table 2, wherein ,-represent The composition is not detected.
Chromatographic condition：Waters e2695 type high performance liquid chromatographs；Chromatographic column：ECOSIL C18（5μm,4.6× 250mm）；Flow velocity：1mL/min；Sample size：30μL.
Table 2：Fry jateorhizine and the red alkali content of medicine root in Cortex Phellodendri, phellodendron bark, Cortex Phellodendri (processed with wine), stir-baked CORTEX PHELLODENDRI with salt solution.
Sample Simultaneous Determinat ion（%） The red alkali content of medicine root（%） Conversion ratio（%）
Fry Cortex Phellodendri 0.023 0.039 26.0
Phellodendron bark 0.150 —
Cortex Phellodendri (processed with wine) 0.390 0.012 8.0
Stir-baked CORTEX PHELLODENDRI with salt solution 0.466 0.023 15.3
As a result show, Simultaneous Determinat ion is significantly more than the content of jateorhizine in phellodendron bark in Cortex Phellodendri (processed with wine), stir-baked CORTEX PHELLODENDRI with salt solution, fries Huang Simultaneous Determinat ion is less than Simultaneous Determinat ion in phellodendron bark in cypress.The red alkali of medicine root is not detected by phellodendron bark, and it is yellow to fry Cortex Phellodendri, wine The red alkali content of medicine root that the red alkali of medicine root is detected in cypress, stir-baked CORTEX PHELLODENDRI with salt solution and generation in product is fried is more, and stir-baked CORTEX PHELLODENDRI with salt solution takes second place, Cortex Phellodendri (processed with wine) It is minimum.Jing after frying, jateorhizine is converted into the conversion ratio highest of the red alkali of medicine root to phellodendron bark.
Embodiment 2.
The present embodiment provides a kind of method for separating and preparing of the red alkali of medicine root for coming from Cortex Phellodendri processed product, comprises the following steps.
Step 1, by Cortex Phellodendri index composition jateorhizine reference substance be simulated process：Precision weighs Jatrorrhizine chloride Reference substance 5mg, in putting evaporating dish, bakes 15min at 160 DEG C, and taking-up is cooled, and adds hplc grade methanol 5mL, microporous filter membrane （0.45μm）Filter, the jateorhizine reference substance solution after must baking carries out the preparation of new component.
Step 2, the separation of jateorhizine monomer baking product new component and preparation.
Preparative liquid chromatography condition：Mobile phase A is water, wherein 100ml water diethylamine containing 0.4ml, and B is methanol；Mobile phase Flow velocity：The mobile phase ratio of A and B is（70:30）；With anti-phase C18Bonded silica stationary phase is chromatographic column filler；25 DEG C of column temperature；It is purple Outer Detection wavelength is 220nm；Sample size is 100 μ L.
Jateorhizine reference substance solution after baking is separated with preparation liquid phase, the eluent of target chromatographic peak is collected simultaneously Jing polydextran gel column purifications；The eluent that purification is crossed carries out nitrogen and blows 15min, and lyophilization at putting -80 DEG C is to be obtained pink Color powder, the red alkali crystallization of medicine root of as separated preparation.
Embodiment 3.
The present embodiment provides a kind of method for separating and preparing of the red alkali of medicine root for coming from Cortex Phellodendri processed product, comprises the following steps.
Step 1, by Cortex Phellodendri index composition jateorhizine reference substance be simulated process：Precision weighs Jatrorrhizine chloride Reference substance 5mg, in putting evaporating dish, bakes 25min at 180 DEG C, and taking-up is cooled, and adds hplc grade methanol 5mL, microporous filter membrane （0.45μm）Filter, the jateorhizine reference substance solution after must baking carries out the preparation of new component.
Step 2, the separation of jateorhizine monomer baking product new component and preparation.
Preparative liquid chromatography condition：Mobile phase A is water, wherein 100ml water diethylamine containing 0.4ml, and B is methanol；Mobile phase Flow velocity：The mobile phase ratio of A and B is（90:10）；With anti-phase C18Bonded silica stationary phase is chromatographic column filler；25 DEG C of column temperature；It is purple Outer Detection wavelength is 265nm；Sample size is 100 μ L.
Jateorhizine reference substance solution after baking is separated with preparation liquid phase, the eluent of target chromatographic peak is collected simultaneously Jing polydextran gel column purifications；The eluent that purification is crossed carries out nitrogen and blows 15min, and lyophilization at putting -80 DEG C is to be obtained pink Color powder, the red alkali crystallization of medicine root of as separated preparation.

Claims (5)

1. a kind of red alkali of medicine root for coming from Cortex Phellodendri processed product, it is characterised in that molecular formula is C19H17NO4, the red alkali of medicine root is named as, Chemical structural formula is as follows
。
2. the method for separating and preparing of the red alkali of medicine root of Cortex Phellodendri processed product is come from as claimed in claim 1, it is characterised in that included Following steps：
Step 1, the simulation of jateorhizine are processed：Precision weighs Jatrorrhizine chloride reference substance, puts in evaporating dish and bakes, and taking-up is cooled, Addition hplc grade methanol, filtering with microporous membrane, filtering with microporous membrane, the aperture of the filter membrane is 0.45 μm, the medicine after must baking Root alkali reference substance solution；
Step 2, the separation of jateorhizine monomer baking product new component and preparation：
Preparative liquid chromatography condition：Mobile phase A is water, wherein 100ml water diethylamine containing 0.4ml, and B is methanol；Flow rate of mobile phase： The mobile phase volume of A and B is than ratio（70-90:30-10）；With anti-phase C18Bonded silica stationary phase is chromatographic column filler；Column temperature 25℃；Ultraviolet detection wavelength is 220-345nm；Sample size is 100 μ L；
Jateorhizine reference substance solution after baking is separated with preparation liquid phase, eluent and the Jing Portugals of target chromatographic peak is collected Polysaccharide gel column purification；The eluent that purification is crossed carries out nitrogen and blows, lyophilization, and pink powder to be obtained is as separated The red alkali crystallization of medicine root of preparation.
3. the method for separating and preparing of the red alkali of medicine root of Cortex Phellodendri processed product is come from as claimed in claim 2, it is characterised in that described Baked temperature is 160-216 DEG C in step 1, and baking time is 15-25min.
4. the method for separating and preparing of the red alkali of medicine root of Cortex Phellodendri processed product is come from as claimed in claim 2, it is characterised in that described The purity for separating the red alkali of medicine root for preparing is more than 98%.
5. coming from the red alkali of medicine root of Cortex Phellodendri processed product as claimed in claim 1 is used to prepare immunoregulatory medicine.
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