CN105533377A - Enzyme beverage and preparation method thereof - Google Patents

Enzyme beverage and preparation method thereof Download PDF

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Publication number
CN105533377A
CN105533377A CN201610016141.1A CN201610016141A CN105533377A CN 105533377 A CN105533377 A CN 105533377A CN 201610016141 A CN201610016141 A CN 201610016141A CN 105533377 A CN105533377 A CN 105533377A
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raw material
parts
enzyme beverage
oxide
deuterium
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丛峰松
徐强
丁运文
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Shanghai Jinweizi Biotechnology Co Ltd
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Shanghai Jinweizi Biotechnology Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A23L2/382—Other non-alcoholic beverages fermented
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses an enzyme beverage. The enzyme beverage is prepared from a raw material, a leavening agent and deuterium-depleted water. The mass ratio of the deuterium-depleted water to the raw material is (1-10):1. The leavening agent comprises saccharomycetes and lactic acid bacteria, the use amount of the saccharomycetes accounts for 5% to 10% of the volume fraction of the inoculated raw material, and the use amount of the lactic acid bacteria accounts for 1% to 3% of the mass of the inoculated raw material. The invention further discloses a preparation method. The preparation method comprises the following steps of 1 raw material pretreatment, 2 saccharomycete fermentation, 3 lactobacillus fermentation and 4 ripening. The enzyme stock solution obtained after ripening can be directly orally taken, and can also be taken after being diluted by 1-10 times with deuterium-depleted water. The enzyme beverage and the preparation method have the advantages that the deuterium-depleted water is adopted as fermentation water, the characteristics that the deuterium-depleted water is high in permeability and the reaction activity of biological enzymes is enhanced are fully utilized, the fermentation period can be obviously shortened, the bioactivator content of the obtained enzyme product is high, the production method is simple in process and suitable for large-scale production, and high economic and social benefits are achieved.

Description

A kind of enzyme beverage and preparation method thereof
Technical field
The invention belongs to technical field of health care food, be specially a kind of enzyme beverage and preparation method thereof.
Background technology
Ferment is also called " enzyme " is the catalyst of various biochemical reaction, and ferment is present in all living cells, is that it starts the vigor of cell, makes cell show kind of a biological phenomena.Enzyme beverage became the new lover in market in recent years.Japan is the country studying ferment the earliest, nowadays the fashionable whole world.Because enzyme beverage contains a large amount of from plant, microbe-derived ferment group and multiple ferment metabolite, the phenomenon that human body endo enzyme that body internal cause food overprocessing and environmental pollution cause generally lacks therefore can be corrected.Large quantity research shows, enzyme beverage has numerous health-care efficacies, and as coordinating intestines and stomach, improvement is digested and assimilated, develop immunitypty, anti-inflammation and sterilization, eliminates free radical, delays senility, anti-cancer, skin maintenance and control body weight etc.
The enzyme beverage occurred in the market, such as publication number is 104137974A, publication number 1047387801A invention disclosed patent, it all discloses fruit, vegetables, mushroom obtains enzyme beverage by fermentation, its beneficiating ingredient all depends on fermentation and obtains, and for example publication number is 104605448A invention disclosed patent, adopt fruit, raw material such as vegetables and add coenzyme element and carry out fermentation and obtain ferment material, there is zymotechnique complexity and need three fermentations in the method, make some technological deficiencies that fermentation period is long, all there is zymotechnique complexity, the shortcoming that fermentation period is long.
Summary of the invention
The present invention only depends on fermentation step obtain beneficiating ingredient to solve enzyme beverage, the technological deficiency of zymotechnique complexity, length consuming time is made with this, a kind of enzyme beverage and preparation method thereof is provided, realize the feature strengthening biological respinse enzymatic activity, the object that method is easy, fermentation period is short, makes to obtain the high enzyme beverage of bioactive ingredients content further while minimizing fermentation step.
In order to reach above-mentioned technique effect, the present invention includes following technical scheme: a kind of enzyme beverage of the present invention's exploitation, comprise raw material, leavening, raw material comprise edible mushroom, algae, veterinary antibiotics, this enzyme beverage also comprises low deuterium-oxide, and low deuterium-oxide and raw material quality are than being 1-10:1; Leavening comprises saccharomycete, lactic acid bacteria, and the amount that saccharomycete uses is for accounting for the 5-10% of inoculation volume of raw material mark, and the amount that lactic acid bacteria uses is for accounting for the 1-3% of inoculation raw material quality; The parts by weight of edible mushroom in raw material are 5-20 part; Parts by weight in algae raw material are 5-20 part.The water existed in nature is generally made up of 2 hydrogen atoms and 1 oxygen atom, but 3 isotopes that hydrogen atom has quality different, atomic weight is respectively 1, hydrogen (H), deuterium (D, heavy hydrogen), the tritium (T, superheavy hydrogen) of 2,3.Domestic and international research shows, deuterium to the survival and development of life entity and procreation harmful, the atomic energy nuclear power station usually mentioned or manufacture the so-called heavy water reactor of atom bomb, heavy water be exactly deuterium-oxide.The water that in water, the content of deuterium commonly uses the content of deuterium in water (nature is commonly used the content of heavy hydrogen in water and is about 150ppm) lower than nature is low deuterium-oxide.Low deuterium-oxide has very important effect in cell activation, cancer-preventing health, disease treatment and cosmetic slimming etc., has many benefits to health.The present invention adopts low deuterium-oxide and edible mushroom, algae, veterinary antibiotics to be mixed and made into beverage, find that low deuterium-oxide mixes harmful effect can't occur with above-mentioned food, find that need not depend on sweat just can make nutritious simultaneously, fermentation time and flow process can be simplified, owing to there being the introducing of low deuterium-oxide, the nutriment in food can be made effectively to dissolve formation beverage, there is effect of coordinating intestines and stomach function, develop immunitypty, anti-ageing and cancer-resisting.
Preferably, edible mushroom comprise mushroom, Hericium erinaceus, Pleurotus ferulae, grifola frondosus, Agricus blazei, pleurotus eryngii and, straw mushroom, flat mushroom, asparagus, glossy ganoderma and auricularia auriculajudae any one or more than one.Edible mushroom contains rich in protein and amino acid.The fungi polysaccharide that edible mushroom is rich in, can stimulate antibody produce and improve body immunity by activated macrophage, have antitumor activity, have very high medicinal health value.Edible mushroom can adopt above-mentioned list one or more, coordinate with low deuterium-oxide and all can form nutritious functional beverage through fermenting.
Preferably, algae comprises any one or more than one combination in brown alga, red algae, green alga.Algae contains abundant bioactive ingredients, and wherein studying more is algal polysaccharides, and have antiviral, antitumor, anti-oxidant, the biologically active such as radioresistance and immunity moderation function, wherein antitumor action especially introduces concern.Such as, sea-tangle in brown alga, undaria pinnitafida, sargassum fusifome etc. contain fucoidin, tool anti-inflammatory, antiviral, activating immune cell, induction cancer cancer cell-apoptosis, and the characteristic of angiogenesis inhibiting.Adopt above-mentioned list one or more, coordinate with low deuterium-oxide and can not make the original disabler of algae through fermenting, with the mutual compatibility of other food source materials, have and further improve the health-care effect such as immunity.
Preferably, vegetables comprise in carrot, tomato, asparagus, wild cabbage one or more, the parts by weight of each composition in raw material are carrot 5-20 part, tomato 5-20 part, wild cabbage 5-20 part, asparagus 5-20 part.The carrotene be rich in carrot improves the immunity of body against cancer and indirectly kills the function of cancer cell; Tomato is containing lycopene.Lycopene is the natural materials that the oxidation resistance that finds so far is strong, can prevention and cure of cardiovascular disease, improve immunity of organisms, pre-anti-cancer; Asparagus is rich in several amino acids, protein and vitamin, its content is all higher than general fruit and vegetables, asparagine particularly in asparagus and trace elements of selenium, molybdenum, chromium, manganese etc., there is adjustment organism metabolism, improve effect of body immunity, in the prevention and therapy to hypertension, heart disease, leukaemia, leukemia, oedema, cystitis etc., there is very strong inhibitory action and pharmacodynamics effect; Brussels sprouts are rich in Yin, can restrain cancer cell hyperplasia, advance toxin expelling, and the toxicity of cancer cell can be become nontoxic after, excrete, and then prevent breast cancer cell from adding, prevent contracting breast cancer.Yin can add body immunity, not only can prevent breast cancer, also to intestinal cancer, cervix cancer, cancer of the stomach, has and prevents effect.Current research shows that eating wild cabbage can reduce the carcinoma of urinary bladder incidence of disease 40% more.Select above-mentioned several vegetables, the effect of vegetables itself can be compatible with other food source materials, the effect that common performance is useful to human body.
Preferably, fruit comprise in apple, pears, hawthorn, pawpaw one or more, the parts by weight of each composition in raw material are apple 5-20 part, pears 5-20 part, hawthorn 5-20 part, red date 5-20 part, pawpaw 5-20 part.Fruit is rich in vitamin C and various trace element, have hypotensive, delaying senility, weight-reducing, skin care, improving eyesight, anticancer, reduce cholesterol and improve the health-care effects such as body immunity.Above-mentioned preferred fruit coordinates with low deuterium-oxide through fermenting, and obtains beverage with the mutual compatibility of other food source materials, has and further improves the health-care effects such as immunity.
Preferably, low deuterium-oxide is mass concentration is 10-137ppm.. when our research finds the low deuterium-oxide when using deuterium content lower than 137ppm, can improve enzyme activity, shortening fermentation time, the enzyme beverage of simultaneously drinking deuterium content when human body can improve physique, and raising immunity of organisms, has anti-cancer effect.Further, the mass concentration of preferred low deuterium-oxide is 30-110ppm.
Further, the invention also discloses the method preparing above-mentioned enzyme beverage, comprise the steps, (1) pre-treatment of raw material: select fresh raw material, cut off after washing and sterilizing, add raw material weight 1-10 low deuterium-oxide making beating doubly;
(2) saccharomycetes to make fermentation: the fruit and vegetable juice pol to be fermented step (1) obtained is adjusted to as 10%-20%, be volume fraction 5-10% according to inoculum concentration, by in the fruit, vegetable juice of Yeast Cultivation liquid access step (1) gained, fermentation temperature is 28-30 DEG C, time 3-5d;
(3) lactobacillus-fermented: according to the 1-3% of inoculation raw material amount, also accessed by lactic acid bacteria in the Juice of step (2), fermentation temperature is 25-37 DEG C, time 3-5d;
(4) maturation: filtering fermentation liquor to be placed in closed container 20-25 DEG C of after-ripening 1-2 month, namely micro-filtrate membrane filtration obtains ferment stoste.Ferment stoste can be directly oral, also can take with after low deuterium-oxide dilution suitable multiple as required.
In above-mentioned preparation method, by means of only saccharomycete and lactic acid bacteria fermenting twice, and time shorten is to 6-10 days, functional component in raw material can be dissolved by the introducing of low deuterium-oxide and obtain enzyme beverage, do not depend on fermentation and obtain beneficiating ingredient, therefore the defect that the Fermentation Engineering time in conventional method of solving is long, even and if also improve that also cannot to realize raw material compatibility by means of only Fermentation Engineering good, the collaborative efficacy exertion that makes arrives best effect.
To sum up, beneficial effect of the present invention is: (1) the present invention selects bacterium mushroom, marine alga, fruits and vegetables to be raw material, science is arranged in pairs or groups, nutritious, the bioactivator that a large amount of human body is easy to absorption is produced by beneficial microbe fermentation, there is coordinating intestines and stomach function, develop immunitypty, effect of anti-ageing and cancer-resisting; (2) the present invention adopts low deuterium-oxide to be fermentation water, making full use of the high feature with strengthening biology enzyme reactivity of low deuterium-oxide permeability, obviously can shorten fermentation period, and the ferment product bioactive substance content obtained is high; (3) production method technique of the present invention is simple, is suitable for large-scale production, has higher economic and social benefit.
Detailed description of the invention
Below by specific embodiment, the present invention is described in further detail.
Embodiment one: a kind of enzyme beverage that the present invention develops, comprise raw material, leavening, raw material comprise edible mushroom, algae, veterinary antibiotics, and this enzyme beverage also comprises low deuterium-oxide, and low deuterium-oxide and raw material quality are than being 1:1; Leavening comprises saccharomycete, lactic acid bacteria, and the amount that saccharomycete uses is for accounting for 5% of inoculation volume of raw material mark, and the amount that lactic acid bacteria uses is for accounting for 1% of inoculation raw material quality; The parts by weight of edible mushroom in raw material are 5 parts; The parts by weight of algae in raw material are 5 parts, the vegetables adopted and fruit can meet the mass ratio of low deuterium-oxide, adopt existing method by raw material stripping and slicing (sheet), cleaning and sterilizing, low deuterium-oxide and raw material are adopted to pull an oar, through saccharomycete, lactobacillus-fermented, namely micro-filtrate membrane filtration obtains ferment stoste.This stoste can directly be drunk, every day 10-30 milliliter.Also the low deuterium-oxide 100ppm of stoste can be diluted 5 times, after modulation mouthfeel, obtain ferment drink.Total acid content >=0.1 after testing, soluble solid content >=1.0, amylase content 16.32U/mL, protease content 220.12U/mL, lipase content 19.32U/mL.Significant humidification is all had to the cellular immunity of normal mouse, humoral immunity after pharmacological evaluation proof ferment stoste dilutes ten times.
One, materials and methods
1.1 animals used as test select cleaning grade female KM kind mouse, and body weight 18g ~ 22g, is divided into four groups at random by body weight: contrast and basic, normal, high dosage group, and often organize 12, test group dosage is respectively 3.3ml/kg, 6.6ml/kg, 10ml/kg body weight.
1.2 test method
1.2.1 cellular immunity adopts mouse delayed allergy test (DTH), spleen lymphocyte conversion test (mtt assay) and Thymus and spleen organ coefficient to measure.
1.2.2 humoral immunity adopts spleen antibody-producting cell to detect (Jerne improves slide method), HD50 value (HC50) measures.
Two, result
2.1 enzyme beverage are on the impact of mice organs coefficient.In table 1.
Group (g/kg.bw) Number of animals (only) Spleen weight/body weight (mg/g) Thymus gland/body weight (mg/g)
Control group (0) 12 4.69±0.78 3.05±0.62
Low dosage (3.3) 12 4.72±1.12 3.22±0.78
Middle dosage (6.6) 12 4.75±1.42 3.26±0.66
High dose (10) 12 4.80±1.63 3.42±1.22
From table 1, per os gives the ferment 30d of mouse various dose, and the organ coefficient Analysis of variance of spleen and thymus gland compares there was no significant difference (P>0.05) between each dosage group.
2.2 enzyme beverage are on the impact of mouse delayed allergy function (DTH).In table 2.
Group (g/kg.bw) Number of animals (only) Swelling degree of the paw difference (mm)
Control group (0) 12 0.52±0.12
Low dosage (3.3) 12 0.58±0.14
Middle dosage (6.6) 12 0.68±0.15*
High dose (10) 12 0.79±0.13**
From table 2, per os gives the ferment 30d of mouse various dose, the swelling degree of the paw of middle and high dosage group and the obvious rising of control group, and has significant difference (P<0.05, P<0.01)
2.3 enzyme beverage are on the impact of the mouse spleen lymphocyte transformation function that ConA induces.In table 3.
From table 3, per os gives the ferment 30d of mouse various dose, along with the increase of dosage, the splenic lymphocyte proliferation ability of mouse strengthens gradually, and it is low, the OD difference of high dose group compares with control group significant difference (P<0.05).
2.4 enzyme beverage are on the impact of mouse spleen antibody-producting cell number.In table 4.
From table 4, per os gives the ferment 30d of mouse various dose, its antibody-producting cell number middle and high dosage group and the obvious rising of control group, and has significant difference (P<0.05, P<0.01).
2.5 enzyme beverage are on the impact of mouse HD50 value (HC50).In table 5.
Group (g/kg.bw) Number of animals (only) HC 50
Control group (0) 12 115.02±18.16
Low dosage (3.3) 12 122.68±16.57
Middle dosage (6.6) 12 132.51±14.26**
High dose (10) 12 141.76±7.66**
From table 5, per os gives the ferment 30d of mouse various dose, the HD50 value of middle and high dosage group and the obvious rising of control group, and has significant difference (P<0.01).
Embodiment two: a kind of enzyme beverage that the present invention develops, comprise raw material, leavening, raw material comprise edible mushroom, algae, veterinary antibiotics, and this enzyme beverage also comprises low deuterium-oxide, and low deuterium-oxide and raw material quality are than being 5:1; Leavening comprises saccharomycete, lactic acid bacteria, and the amount that saccharomycete uses is for accounting for 8% of inoculation volume of raw material mark, and the amount that lactic acid bacteria uses is for accounting for 2% of inoculation raw material quality; The parts by weight of edible mushroom in raw material are 10 parts; The parts by weight of algae in raw material are 15 parts, and vegetables comprise carrot, tomato, asparagus, wild cabbage, and the parts by weight of each composition in raw material are 20 parts, carrot, tomato 5 parts, wild cabbage 20 parts, asparagus 5 parts.
Described edible mushroom can adopt mushroom, Hericium erinaceus, Pleurotus ferulae, grifola frondosus, Agricus blazei, pleurotus eryngii and, straw mushroom, flat mushroom, asparagus, glossy ganoderma and auricularia auriculajudae any one or more than one.
Algae can adopt any one or more than one combination in brown alga, red algae, green alga.
Fruit comprise in apple, pears, hawthorn, pawpaw one or more, the parts by weight of each composition in raw material are apple 5 parts, pears 20 parts, hawthorn 5 parts, red date 5-20 part, pawpaw 5 parts.
Described low deuterium-oxide is mass concentration is 10ppm.
The method preparing above-mentioned enzyme beverage comprises the steps, (1) pre-treatment of raw material: select fresh raw material, cut off after washing and sterilizing, adds the low deuterium-oxide making beating of raw material weight 5 times;
(2) saccharomycetes to make fermentation: the fruit and vegetable juice pol to be fermented step (1) obtained is adjusted to as 10%-20%, be volume fraction 8% according to inoculum concentration, by in the fruit, vegetable juice of Yeast Cultivation liquid access step (1) gained, fermentation temperature is 28-30 DEG C, time 3-5d;
(3) lactobacillus-fermented: according to 2% of inoculation raw material amount, also accessed by lactic acid bacteria in the Juice of step (2), fermentation temperature is 28-30 DEG C, time 3-5d;
(4) maturation: filtering fermentation liquor to be placed in closed container 20-25 DEG C of after-ripening 1-2 month, namely micro-filtrate membrane filtration obtains ferment stoste.Ferment stoste can be directly oral, also can take with after low deuterium-oxide dilution suitable multiple as required.
Obtain above-mentioned beverage, every day is drunk 10-30 milliliter, total acid content >=0.1 after testing, soluble solid content >=1.0, amylase content 17.24U/mL, protease content 210.23U/mL, lipase content 20.02U/mL in suggestion.Significant humidification is all had to the cellular immunity of normal mouse, humoral immunity after pharmacological evaluation proof ferment stoste dilutes ten times.
Embodiment three: a kind of enzyme beverage that the present invention develops, comprise raw material, leavening, raw material comprise edible mushroom, algae, veterinary antibiotics, and this enzyme beverage also comprises low deuterium-oxide, and low deuterium-oxide and raw material quality are than being 8:1; Leavening comprises saccharomycete, lactic acid bacteria, and the amount that saccharomycete uses is for accounting for 6% of inoculation volume of raw material mark, and the amount that lactic acid bacteria uses is for accounting for 1.5% of inoculation raw material quality; The parts by weight of edible mushroom in raw material are 10 parts; The parts by weight of algae in raw material are 15 parts, and vegetables comprise carrot, tomato, asparagus, wild cabbage, and the parts by weight of each composition in raw material are 20 parts, carrot, tomato 5 parts, wild cabbage 20 parts, asparagus 5 parts.
Described edible mushroom can adopt mushroom, Hericium erinaceus, Pleurotus ferulae, grifola frondosus, Agricus blazei, pleurotus eryngii and, straw mushroom, flat mushroom, asparagus, glossy ganoderma and auricularia auriculajudae any one or more than one.
Algae can adopt any one or more than one combination in brown alga, red algae, green alga.
Fruit comprise in apple, pears, hawthorn, pawpaw one or more, the parts by weight of each composition in raw material are apple 5 parts, pears 20 parts, hawthorn 5 parts, red date 5-20 part, pawpaw 5 parts.
Described low deuterium-oxide is mass concentration is 10ppm.
The method preparing above-mentioned enzyme beverage comprises the steps, (1) pre-treatment of raw material: select fresh raw material, cut off after washing and sterilizing, adds the low deuterium-oxide making beating of raw material weight 8 times;
(2) saccharomycetes to make fermentation: the fruit and vegetable juice pol to be fermented step (1) obtained is adjusted to as 10%-20%, be volume fraction 6% according to inoculum concentration, by in the fruit, vegetable juice of Yeast Cultivation liquid access step (1) gained, fermentation temperature is 28-30 DEG C, time 3-5d;
(3) lactobacillus-fermented: according to 1.5% of inoculation raw material amount, also accessed by lactic acid bacteria in the Juice of step (2), fermentation temperature is 28-30 DEG C, time 3-5d;
(4) maturation: filtering fermentation liquor to be placed in closed container 20-25 DEG C of after-ripening 1-2 month, namely micro-filtrate membrane filtration obtains ferment stoste.Ferment stoste can be directly oral, also can take with after low deuterium-oxide dilution suitable multiple as required.
Obtain above-mentioned beverage, every day is drunk 10-30 milliliter, total acid content >=0.1 after testing, soluble solid content >=1.0, amylase content 12.32U/mL, protease content 233.42U/mL, lipase content 15.11U/mL in suggestion.Significant humidification is all had to the cellular immunity of normal mouse, humoral immunity after pharmacological evaluation proof ferment stoste dilutes ten times.
Embodiment four: a kind of enzyme beverage that the present invention develops, comprise raw material, leavening, raw material comprise edible mushroom, algae, veterinary antibiotics, and this enzyme beverage also comprises low deuterium-oxide, and low deuterium-oxide and raw material quality are than being 2:1; Leavening comprises saccharomycete, lactic acid bacteria, and the amount that saccharomycete uses is for accounting for 7% of inoculation volume of raw material mark, and the amount that lactic acid bacteria uses is for accounting for 2.5% of inoculation raw material quality; The parts by weight of edible mushroom in raw material are 10 parts; The parts by weight of algae in raw material are 15 parts, and vegetables comprise carrot, tomato, asparagus, wild cabbage, and the parts by weight of each composition in raw material are 5 parts, carrot, tomato 20 parts, wild cabbage 5 parts, asparagus 20 parts.
Described edible mushroom can adopt mushroom, Hericium erinaceus, Pleurotus ferulae, grifola frondosus, Agricus blazei, pleurotus eryngii and, straw mushroom, flat mushroom, asparagus, glossy ganoderma and auricularia auriculajudae any one or more than one.
Algae can adopt any one or more than one combination in brown alga, red algae, green alga.
Fruit comprise in apple, pears, hawthorn, pawpaw one or more, the parts by weight of each composition in raw material are apple 20 parts, pears 5 parts, hawthorn 20 parts, red date 5 parts, pawpaw 20 parts.
Described low deuterium-oxide is mass concentration is 137ppm.
The method preparing above-mentioned enzyme beverage comprises the steps, (1) pre-treatment of raw material: select fresh raw material, cut off after washing and sterilizing, adds the low deuterium-oxide making beating of raw material weight 2 times;
(2) saccharomycetes to make fermentation: the fruit and vegetable juice pol to be fermented step (1) obtained is adjusted to as 10%-20%, be volume fraction 7% according to inoculum concentration, by in the fruit, vegetable juice of Yeast Cultivation liquid access step (1) gained, fermentation temperature is 28-30 DEG C, time 3-5d;
(3) lactobacillus-fermented: according to 2.5% of inoculation raw material amount, also accessed by lactic acid bacteria in the Juice of step (2), fermentation temperature is 28-30 DEG C, time 3-5d;
(4) maturation: filtering fermentation liquor to be placed in closed container 20-25 DEG C of after-ripening 1-2 month, namely micro-filtrate membrane filtration obtains ferment stoste.Ferment stoste can be directly oral, also can take with after low deuterium-oxide dilution suitable multiple as required.
Obtain above-mentioned beverage, every day is drunk 10-30 milliliter, total acid content >=0.1 after testing, soluble solid content >=1.0, amylase content 12.32U/mL, protease content 233.42U/mL, lipase content 15.11U/mL in suggestion.Significant humidification is all had to the cellular immunity of normal mouse, humoral immunity after pharmacological evaluation proof ferment stoste dilutes ten times.
Embodiment five: a kind of enzyme beverage that the present invention develops, comprise raw material, leavening, raw material comprise edible mushroom, algae, veterinary antibiotics, and this enzyme beverage also comprises low deuterium-oxide, and low deuterium-oxide and raw material quality are than being 9:1; Leavening comprises saccharomycete, lactic acid bacteria, and the amount that saccharomycete uses is for accounting for 9% of inoculation volume of raw material mark, and the amount that lactic acid bacteria uses is for accounting for 2.5% of inoculation raw material quality; The parts by weight of edible mushroom in raw material are 10 parts; The parts by weight of algae in raw material are 15 parts, and vegetables comprise carrot, tomato, asparagus, wild cabbage, and the parts by weight of each composition in raw material are 10 parts, carrot, tomato 15 parts, wild cabbage 17 parts, asparagus 12 parts.
Described edible mushroom can adopt mushroom, Hericium erinaceus, Pleurotus ferulae, grifola frondosus, Agricus blazei, pleurotus eryngii and, straw mushroom, flat mushroom, asparagus, glossy ganoderma and auricularia auriculajudae any one or more than one.
Algae can adopt any one or more than one combination in brown alga, red algae, green alga.
Fruit comprise in apple, pears, hawthorn, pawpaw one or more, the parts by weight of each composition in raw material are apple 15 parts, pears 10 parts, hawthorn 12 parts, red date 19 parts, pawpaw 8 parts.
Described low deuterium-oxide is mass concentration is 60ppm.
The method preparing above-mentioned enzyme beverage comprises the steps, (1) pre-treatment of raw material: select fresh raw material, cut off after washing and sterilizing, adds the low deuterium-oxide making beating of raw material weight 9 times;
(2) saccharomycetes to make fermentation: the fruit and vegetable juice pol to be fermented step (1) obtained is adjusted to as 10%-20%, be volume fraction 9% according to inoculum concentration, by in the fruit, vegetable juice of Yeast Cultivation liquid access step (1) gained, fermentation temperature is 28-30 DEG C, time 3-5d;
(3) lactobacillus-fermented: according to 2.5% of inoculation raw material amount, also accessed by lactic acid bacteria in the Juice of step (2), fermentation temperature is 28-30 DEG C, time 3-5d;
(4) maturation: filtering fermentation liquor to be placed in closed container 20-25 DEG C of after-ripening 1-2 month, namely micro-filtrate membrane filtration obtains ferment stoste.Ferment stoste can be directly oral, also can take with after low deuterium-oxide dilution suitable multiple as required.
Obtain above-mentioned beverage, every day is drunk 10-30 milliliter, total acid content >=0.1 after testing, soluble solid content >=1.0, amylase content 14.21U/mL, protease content 227.32U/mL, lipase content 15.03U/mL in suggestion.Significant humidification is all had to the cellular immunity of normal mouse, humoral immunity after pharmacological evaluation proof ferment stoste dilutes ten times.
Embodiment six: a kind of enzyme beverage that the present invention develops, comprise raw material, leavening, raw material comprise edible mushroom, algae, veterinary antibiotics, and this enzyme beverage also comprises low deuterium-oxide, and low deuterium-oxide and raw material quality are than being 9:1; Leavening comprises saccharomycete, lactic acid bacteria, and the amount that saccharomycete uses is for accounting for 9% of inoculation volume of raw material mark, and the amount that lactic acid bacteria uses is for accounting for 2.5% of inoculation raw material quality; The parts by weight of edible mushroom in raw material are 10 parts; The parts by weight of algae in raw material are 15 parts, and vegetables comprise carrot, tomato, asparagus, wild cabbage, and the parts by weight of each composition in raw material are 6 parts, carrot, tomato 19 parts, wild cabbage 12 parts, asparagus 14 parts.
Described edible mushroom can adopt mushroom, Hericium erinaceus, Pleurotus ferulae, grifola frondosus, Agricus blazei, pleurotus eryngii and, straw mushroom, flat mushroom, asparagus, glossy ganoderma and auricularia auriculajudae any one or more than one.
Algae can adopt any one or more than one combination in brown alga, red algae, green alga.
Fruit comprise in apple, pears, hawthorn, pawpaw one or more, the parts by weight of each composition in raw material are apple 7 parts, pears 18 parts, hawthorn 8 parts, red date 14 parts, pawpaw 13 parts.
Described low deuterium-oxide is mass concentration is 100ppm.
The method preparing above-mentioned enzyme beverage comprises the steps, (1) pre-treatment of raw material: select fresh raw material, cut off after washing and sterilizing, adds the low deuterium-oxide making beating of raw material weight 9 times;
(2) saccharomycetes to make fermentation: the fruit and vegetable juice pol to be fermented step (1) obtained is adjusted to as 10%-20%, be volume fraction 9% according to inoculum concentration, by in the fruit, vegetable juice of Yeast Cultivation liquid access step (1) gained, fermentation temperature is 28-30 DEG C, time 3-5d;
(3) lactobacillus-fermented: according to 2.5% of inoculation raw material amount, also accessed by lactic acid bacteria in the Juice of step (2), fermentation temperature is 28-30 DEG C, time 3-5d;
(4) maturation: filtering fermentation liquor to be placed in closed container 20-25 DEG C of after-ripening 1-2 month, namely micro-filtrate membrane filtration obtains ferment stoste.Ferment stoste can be directly oral, also can take with after low deuterium-oxide dilution suitable multiple as required.
Obtain above-mentioned beverage, every day is drunk 10-30 milliliter, total acid content >=0.1 after testing, soluble solid content >=1.0, amylase content 20.34U/mL, protease content 289.13U/mL, lipase content 16.42U/mL in suggestion.Significant humidification is all had to the cellular immunity of normal mouse, humoral immunity after pharmacological evaluation proof ferment stoste dilutes ten times.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (8)

1. an enzyme beverage, comprises raw material, leavening, and raw material comprise edible mushroom, algae, veterinary antibiotics, it is characterized in that, this enzyme beverage also comprises low deuterium-oxide, and low deuterium-oxide and raw material quality are than being 1-10:1; Leavening comprises saccharomycete, lactic acid bacteria, and the amount that saccharomycete uses is for accounting for the 5-10% of inoculation volume of raw material mark, and the amount that lactic acid bacteria uses is for accounting for the 1-3% of inoculation raw material quality; The parts by weight of edible mushroom in raw material are 5-20 part; The parts by weight of algae in raw material are 5-20 part.
2. enzyme beverage according to claim 1, is characterized in that, edible mushroom comprise mushroom, Hericium erinaceus, Pleurotus ferulae, grifola frondosus, Agricus blazei, pleurotus eryngii and, straw mushroom, flat mushroom, asparagus, glossy ganoderma and auricularia auriculajudae any one or more than one.
3. enzyme beverage according to claim 1, is characterized in that, algae comprises any one or more than one combination in brown alga, red algae, green alga.
4. enzyme beverage according to claim 1, it is characterized in that, vegetables comprise in carrot, tomato, asparagus, wild cabbage one or more, the parts by weight of each composition in raw material are carrot 5-20 part, tomato 5-20 part, wild cabbage 5-20 part, asparagus 5-20 part.
5. enzyme beverage according to claim 1, it is characterized in that, fruit comprise in apple, pears, hawthorn, pawpaw one or more, the parts by weight of each composition in raw material are apple 5-20 part, pears 5-20 part, hawthorn 5-20 part, red date 5-20 part, pawpaw 5-20 part.
6. enzyme beverage according to claim 1, is characterized in that, low deuterium-oxide is mass concentration is 10-137ppm.
7. prepare a method for enzyme beverage described in claim 1, it is characterized in that, the method comprises the steps, (1) pre-treatment of raw material: select fresh raw material, cut off after washing and sterilizing, adds raw material weight 1-10 low deuterium-oxide making beating doubly;
(2) saccharomycetes to make fermentation: the fruit and vegetable juice pol to be fermented step (1) obtained is adjusted to as 10%-20%, be volume fraction 5-10% according to inoculum concentration, by in the fruit, vegetable juice of Yeast Cultivation liquid access step (1) gained, fermentation temperature is 28-30 DEG C, time 3-5d;
(3) lactobacillus-fermented: according to the 1-3% of inoculation raw material amount, also accessed by lactic acid bacteria in the Juice of step (2), fermentation temperature is 25-37 DEG C, time 3-5d;
(4) maturation: filtering fermentation liquor to be placed in closed container 20-25 DEG C of after-ripening 1-2 month, namely micro-filtrate membrane filtration obtains ferment stoste.
8. the method preparing enzyme beverage according to claim 7, dilutes with low deuterium-oxide after obtaining ferment stoste.
CN201610016141.1A 2016-01-11 2016-01-11 Enzyme beverage and preparation method thereof Pending CN105533377A (en)

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CN105962321A (en) * 2016-05-10 2016-09-28 天津膳方缘科技有限公司 Preparation method of lucid ganoderma fermentation solution
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CN108056347A (en) * 2018-01-09 2018-05-22 安徽妙奇树生物科技有限公司 A kind of hornwort enzyme beverage and preparation method thereof
CN108201043A (en) * 2016-12-19 2018-06-26 吉林农业大学 A kind of mushroom ferment composite beverage secondary fermentation production technology
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CN108902318A (en) * 2018-08-13 2018-11-30 安徽国科生物科技有限公司 The method that a kind of low deuterium-oxide of rich trace elements prepares small molecular protein peptide beverage
CN109077203A (en) * 2018-08-18 2018-12-25 安徽国科生物科技有限公司 Micro- drinks of a kind of low deuterium-oxide richness and preparation method thereof
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CN105962321A (en) * 2016-05-10 2016-09-28 天津膳方缘科技有限公司 Preparation method of lucid ganoderma fermentation solution
CN107409742A (en) * 2016-05-23 2017-12-01 浙江泛亚生物医药股份有限公司 A kind of artificial culture method of cicada fungus
CN105919109A (en) * 2016-06-08 2016-09-07 福建国粮食品股份有限公司 Preparation method of shaddock leaven and shaddock peel leaven
CN106213095A (en) * 2016-06-30 2016-12-14 恒枫食品科技有限公司 The preparation method of a kind of fermented fruits and vegetables juice and obtain the application of product
CN106235320A (en) * 2016-09-26 2016-12-21 黄玉捷 A kind of processing method of ferment stock solution
CN106578794A (en) * 2016-12-01 2017-04-26 李恒 Fermented fruit and vegetable juice beverage preparation process
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CN107536061A (en) * 2017-07-27 2018-01-05 贵州源熙生物研发有限公司 Compound probiotic dietary fiber with and preparation method thereof
CN107964482A (en) * 2017-10-18 2018-04-27 湖州新驰医药科技有限公司 A kind of production method of compound edible mushroom wine
CN107723184A (en) * 2017-10-18 2018-02-23 湖州新驰医药科技有限公司 A kind of production method of edible mushroom wine
CN107964481A (en) * 2017-10-18 2018-04-27 湖州新驰医药科技有限公司 The production method of compound edible mushroom wine
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CN109090579A (en) * 2018-07-03 2018-12-28 玖捌壹健康科技集团有限公司 Significantly improve the nutritional supplement and methods and applications of human health level
CN108902318A (en) * 2018-08-13 2018-11-30 安徽国科生物科技有限公司 The method that a kind of low deuterium-oxide of rich trace elements prepares small molecular protein peptide beverage
CN109090394A (en) * 2018-08-13 2018-12-28 安徽国科生物科技有限公司 A kind of preparation method preventing and treating four high low deuterium-oxide beverages
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