CN105494321B - A kind of seed deep-frozen storage method - Google Patents
A kind of seed deep-frozen storage method Download PDFInfo
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- CN105494321B CN105494321B CN201610015376.9A CN201610015376A CN105494321B CN 105494321 B CN105494321 B CN 105494321B CN 201610015376 A CN201610015376 A CN 201610015376A CN 105494321 B CN105494321 B CN 105494321B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The present invention provides a kind of seed deep-freezing processing and storing methods, it is characterised in that：Seed is placed on gauze wrapped in freezing liquid, freezing 2 15 minutes is impregnated at a temperature of 15 DEG C~30 DEG C, after taking out seed, removes gauze, seed pelleting is carried out, packs and be put into stored frozen in seed bank, or is placed in the shady and cool warehouse of ventilation and is stored.This method has many advantages, such as that equipment cost is low, section space, speed is fast, easy to operate and floor space is small.Using this method treated seed the is rapidly reached storages condition such as drying and dehydrating requirement, and seed vitality can be improved, extend seed longeivity, increase the resistance of seed and plant, it is finally reached the effect of increasing both production and income, can solve the problems, such as that drying process and temperature humidity are difficult to co- controlling in existing storing technology.
Description
Technical field
This patent belongs to vegetable seeds storage related field, handles non-vernalization crop seeds, kind that treated
Son can be rapidly reached the requirement of the storages condition such as drying and dehydrating, and keep higher seed vitality.This method has at low cost, section sky
Between, the advantages that speed is fast and operating process is easy.
Background technology
Seed is the main material of agricultural production, and the quality of seed quality directly affects the efficiency and income of production.Seed
Service life is different in size, and the seed of cereal crops and industrial crops is mostly short life seed, and seed and germ plasm resource are in storage
Deterioration easy to aging in the process, this brings difficulty to seed and Germ-plasma resources protection, not only influences the work of breeding units and can also increase
The risk and cost of seed addition operating unit is put into.Country increasingly payes attention to the construction of seed freezer, and it is new all to dial huge fund every year
It builds the special freezer of batch of seeds and carries out Germ-plasma resources protection, each seeds company is also required to build specific warehouse, stores commission
The purpose of seed, storage is in order to keep the vigor of seed, to adapt to the needs of production.The basic principle of storage be low temperature,
Under conditions of drying, the respiratory intensity of seed is reduced as possible, nutrient consumption is reduced, to keep the vitality of seed.
The shelf-life of seed as general agricultural and sideline product, by shadows such as itself moisture, the temperature of storage environment, humidity
It rings.Seed also requires to keep corresponding temperature and humidity in library into should carry out necessary drying before library.Currently, China
Traditional Germplasm Bank conservation object is crops and its nearly edge wild plant germ plasm resource, these resources are using seed as germplasm
Carrier, seed can low temperature resistant and resistance to drying and dehydrating.Specific method：First, Germplasm Bank is after being received into seed, it need to be to seed
Cleaned, viability detection, the storages such as drying and dehydrating preserve pre-treatment, then pack -18 DEG C of freezer (temperature-of deposit
18 DEG C ± 1 DEG C, relative humidity<50%).The initial emergence rate that storage preserves seed generally requires to be higher than 90%, seed moisture content
Drying and dehydrating is to 5%~7%.It is estimated that under above-mentioned holding conditions, the general crop seed service life can save 50 years or more.But
It is the optimum storaging condition for how determining seed, update preservation sample, the genetic stability for monitoring germplasm, is all in the world
The problem that seed bank faces.Meanwhile the highly energy-consuming of Freezing room limits the construction and operation of seed bank.Second is that cryopreservation.
Plant germplasm resource cryopreservation is usually in the ultralow of liquid nitrogen (- 196 DEG C) and liquid nitrogen vapor phase (- 150~-180 DEG C)
Plant cell, tissue or organ are preserved under the conditions of temperature.Vegetable material at such lower temperatures, adjusts and controls cell growth generation
The effect for the enzyme classes thanked is greatly suppressed, and metabolic activity stops substantially, is in " life pause " or " seemingly-dead " state.
After cryopreservation, in " seemingly-dead " state vegetable material can keep the activity of normal cell, form occur potential and
Genetic stability, to achieve the purpose that long-term preservation vegetable material.But tolerance and plant of the vegetable material to low temperature
Kind, genotype and organ, tissue and cell physiological status have substantial connection.Different vegetable materials are with same ultralow
Result is also different after warm method preserves, and instrument when needing to establish different cryopreservation systems, while building library early period is set
Standby input spends also larger.
Therefore, if seed drying, dehydration and temperature humidity are controlled one using a kind of simple, low cost method
Step is completed, and will be the effective way of seed and germ plasm resource storage.And deep-frozen preservation technology is carried out to each substance
In processing procedure, dehydration is completed at the same time to the substance being placed in fresh-keeping freezing liquid, which fundamentally solves kind
The defect in matter library and cryopreservation, and can ensure the vigor of vegetable seeds, form green bio technologic material effectively, peace
Entirely, the method for stabilization, economy, long-term preservation.
Invention content
In order to solve the above problem, the present invention provides a kind of seed deep-freezings to process storing technology, specially：Seed is used
Gauze wrapped is placed in freezing liquid, at a temperature of -15 DEG C~-30 DEG C impregnate freezing 2-15 minute (most preferred scheme for -
Freezing 8 minutes is impregnated at a temperature of 20 DEG C), after taking out seed, remove gauze, carries out seed pelleting, packs and be put into seed bank
Stored frozen, or be placed in the shady and cool warehouse of ventilation and stored.
Seed deep-frozen storage method of the present invention, which is characterized in that the ingredient and weight hundred of the freezing liquid
Point ratio is：
Wherein, the ethyl alcohol that ethyl alcohol is a concentration of 98%, the sucrose are edible sucrose.
Most preferably proportioning is：Sodium chloride 15%, a concentration of 98% ethyl alcohol 26%, glycerine 23%, edible sucrose 5%, first
Shell element 6%, remaining is water.
Freezing liquid adjustable temperature of the present invention ranging from room temperature is to -60 DEG C.
Seed deep-frozen storage method of the present invention, it is characterised in that：This method is suitable for cereal crops, economy
The storage of non-vernalization crop seeds in crop.
Seed deep-frozen storage method of the present invention, section space low with equipment cost, speed is fast, operation is simple
Just the advantages that and floor space is small.Using deep-frozen method, treated that seed is rapidly reached the storages condition such as drying and dehydrating
It is required that and can be improved seed vitality, extend seed longeivity, increase the resistance of seed and plant, be finally reached the effect of increasing both production and income
Fruit can solve the problems, such as that drying process and temperature humidity are difficult to co- controlling in existing storing technology.
Description of the drawings
The seedling of Fig. 1 freezing processings.
The seedling of Fig. 2 room temperature processing.
The plant of Fig. 3 freezing processings.
The plant of Fig. 4 room temperature processing.
The plant of Fig. 5 freezing processings.
The plant of Fig. 6 room temperature processing.
Fig. 7 fruit ears (left figure is the fruit ear of low-temperature treatment, and right figure is the fruit ear of room temperature processing).
Fig. 8 routine cucumbers.
Fig. 9 freeze preservations handle cucumber.
Figure 10 routine cucumber maturity periods.
Figure 11 freeze preservations handle the cucumber maturity period.
Specific implementation mode
In order to verify effect of the invention in the processing storage of seed freeze cutting, we are respectively to corn and cucumber seeds
Working process is carried out, concrete condition such as embodiment 1 and embodiment 2 describe.
Embodiment 1：
Seeding ratio, plant growing way are influenced after the processing storage of corn seed freeze cutting, we choose suitable black
The main cultivation corn variety of each snow change of Longjiang province amounts to 12 (2 main breeds of each snow change) and is tested and (be shown in Table 1).
Table 1 chooses varieties of plant
Test method：
12 parts of corn hybrid seeds are subjected to deep-frozen processing.Processing procedure is the corn seed that will select -15
DEG C freezing liquid in freezing impregnate 6 minutes, freezing liquid is wiped after taking-up, is put into conventional refrigerator stored refrigerated 3 months.When plantation
Monoseeding, seed carry out 2 kinds of processing methods, and first group of seed for profound hypothermia processing, second group is untreated seed.Institute
It is with freezing liquid ingredient and weight percent：
By experiment material kind, seedling stage carries out growth of cereal crop seedlings observation in farmland, and determination of yield is carried out after ripe.Each test material kind
It plants in the Heilongjiang Province Shangzhi City experimental plots Mao Ershan and Heilongjiang Province Suihua City Yongan Manchu town experimental plot, cell 4m rows are long,
0.6m line width, spacing in the rows 0.25m, 4 row areas, 17 plants of often row plantation, 2 repetitions, normal field management.According to《National conventional corn
Regional experiment of variety, pilot production, pilot study survey item and standard》(2006) go out each test material seedling stage
Seedling rate is investigated, and the jointing stage carries out plant forms observation, and milk stage kataphase is investigated into the fields such as every trade plant height, growing way character, at
The ripe phase carries out determination of yield to respectively testing material, records data.
Experiment material is planted in Heilongjiang Province Suihua Yongan Manchu town and the Heilongjiang Province Shangzhi City experimental plots Mao Ershan, is gone out
Seeding ratio investigation is carried out to each 192 experimental plots under two environment after seedling, the 3-4 leaf phases carry out seedling growth investigation, pull out
The section phase investigates corn growing way, and milking maturity latter stage investigates corn growing way, to corn yield investigate after harvest
To following result：
Seeding ratio investigation (being shown in Table 2) is carried out to each 192 experimental plots under two environment after emergence, is found：It carries out
Its emergence rate of the seed of low-temperature treatment and the seeding ratio that room temperature is stored are substantially close.Experiment explanation, seed is by low temperature
After reason, vigor is basically unchanged.
Respectively processing seed is averaged emergence rate table 2
| Processing mode | Room temperature | Freezing |
| Seed is averaged emergence rate | 90.22 | 90.27 |
Consistency analysis is carried out to 12 material emergence rates, it is higher that analysis result shows that different cultivars emergence rate has
Consistency, seed variety influence cryogenic freezing effect little.It can thus be concluded that be applicable to Heilongjiang Province big for cryogenic freezing method
Most corn main breeds.
The 3-4 leaf phases investigate (see Fig. 1,2) to seedling growth, known to relatively：Test material after cryogenic freezing with not
The material of freezing is compared, and some materials growing way is more than the material not freezed；
Jointing stage investigates (see Fig. 3,4) to seedling growth, the plant that seed is grown by freezing compared with the plant of room temperature,
Most of material straw stiffness and plant height are better than room temperature plant；Plant growth situation after cryogenic freezing is good, preliminary aobvious
The effect that may increase production is shown.
Milking maturity latter stage investigates (see Fig. 5,6) to plant growing way, and chilled growth situation is good, is better than not chilled material
Material.
Maturity period carries out field and surveys production, the results are shown in Table 3.It can be seen that freezing processing and room temperature processing from table 3 and Fig. 7
Material is compared, in addition to CS5101, the imperial material for educating 9 and Feng He, 6 three material room temperature processing chilled processing of material ratio are slightly higher,
Yu Jun is slightly below the material of chilled processing.
The average spike weight amount (unit of 2 kinds of 3 field of table processing：Gram)
Embodiment 2:
In order to examine or check cucumber seeds through cryogenic freezing treated growing state, we have selected the Dalian institute of agricultural sciences to cultivate
09-11 even tested by elegant cucumber breeding.First use deep-frozen of the present invention processing method to cucumber seeds into
Row cryogenic freezing processing, then by cryogenic freezing processing and no processing cucumber seeds it is same cultivation plot two regions into
Row plantation, plantation terms and conditions are consistent.Can be seen that from the growth course of cucumber seeds the seed that is handled through cryogenic freezing its
Growing way, which has more than, does not have processed seed (see Fig. 8-11).
Embodiment 3：
Water intaking rice, soybean and pepper seed, two groups are divided by each seed：Cryogenic freezing processing group and untreated fish group,
The seed of cryogenic freezing processing group is placed on gauze wrapped in freezing liquid, freezing 8 minutes is impregnated at -20 DEG C, takes out kind
After son, remove gauze, carries out seed pelleting, packs and be put into conventional refrigerator stored refrigerated 3 months.The freezing liquid ingredient and
Weight percent is：Sodium chloride 15%, a concentration of 98% ethyl alcohol 26%, glycerine 23%, edible sucrose 5%, chitin 6%,
Remaining is water.
By the seed of same type cryogenic freezing processing group and untreated fish group respectively it is same cultivation plot two regions into
Row plantation, plantation terms and conditions are consistent.It can be seen that the seed that is handled through cryogenic freezing its length from the growth course of each plant
Gesture has more than untreated seed.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of seed deep-frozen storage method, it is characterised in that：Seed is placed on gauze wrapped in freezing liquid,
Freezing 2-15 minutes is impregnated at a temperature of -15 DEG C~-30 DEG C, after taking out seed, removes gauze, carries out seed pelleting, packaging simultaneously
It is put into stored frozen in seed bank, or is placed in the shady and cool warehouse of ventilation and is stored；
The ingredient and weight percent of the freezing liquid be：
Remaining is water；
The seed is non-vernalization crop seeds in cereal crops, industrial crops.
2. according to seed deep-frozen storage method described in claim 1, it is characterised in that：The ethyl alcohol is a concentration of 98%
Ethyl alcohol, the sucrose be edible sucrose.
3. according to seed deep-frozen storage method described in claim 1, it is characterised in that：The composition of the freezing liquid and
Composition proportion is：Sodium chloride 15%, a concentration of 98% ethyl alcohol 26%, glycerine 23%, edible sucrose 5%, chitin 6%,
Yu Weishui.
4. according to seed deep-frozen storage method described in claim 1, it is characterised in that：The freezing liquid adjustable temperature model
It encloses for room temperature to -60 DEG C.
5. according to seed deep-frozen storage method described in claim 1, it is characterised in that：Seed soaks at a temperature of -20 DEG C
Bubble freezing 8 minutes.
6. a kind of purposes of claim 1 the method, it is characterised in that：This method is suitable for cereal crops, industrial crops
The storage of non-vernalization crop seeds.
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| CN201610015376.9A CN105494321B (en) | 2016-01-11 | 2016-01-11 | A kind of seed deep-frozen storage method |
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| CN105494321B true CN105494321B (en) | 2018-09-21 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106258070B (en) * | 2016-08-10 | 2017-07-28 | 国俊伍 | A kind of seed freezing storage device and method |
| CN107182362A (en) * | 2017-06-30 | 2017-09-22 | 李兴国 | A kind of device and its processing method for activating crop seeds antifreeze glycoprotein |
| CN107593814A (en) * | 2017-09-22 | 2018-01-19 | 浙江智链生物科技有限公司 | A kind of vegetable seeds low temperature induction liquid |
| CN108812650A (en) * | 2018-07-18 | 2018-11-16 | 安徽省金海种业有限公司 | A kind of Cryopreservation of cereal seed |
| CN112930747B (en) * | 2021-01-20 | 2022-03-11 | 苏州三亩良铺农业科技有限公司 | Crop seed micro-freezing method and application thereof |
| CN113142050B (en) * | 2021-03-30 | 2022-07-22 | 苏州蓝润农业科技有限公司 | Treatment method and tissue culture process of blueberry branch explants |
| CN113331052B (en) * | 2021-03-30 | 2022-07-22 | 苏州蓝润农业科技有限公司 | Process for cultivating superior blueberry products by using micro-freezing biotechnology |
| CN114557166A (en) * | 2022-03-16 | 2022-05-31 | 上海道和邦电子商务有限公司 | Low-temperature treatment yield increasing method for crops |
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| CN101810072B (en) * | 2010-05-12 | 2011-09-28 | 西北农林科技大学 | Method for treating sea-buckthorn seeds before sowing |
| CN102301890A (en) * | 2011-07-20 | 2012-01-04 | 易思荣 | Technology for propagating Artocarpus nanchuanensis seeds |
| CN103689061B (en) * | 2013-12-05 | 2015-10-14 | 大连博缘科技有限公司 | A kind of cell freezing fresh-keeping liquid |
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