CN103229762A - Leucaena leucocephala seed ultra-dry preservation method - Google Patents
Leucaena leucocephala seed ultra-dry preservation method Download PDFInfo
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Abstract
The invention belongs to the technical field of seed ultra-dry preservation, and provides a leucaena leucocephala seed ultra-dry preservation method. According to the invention, silica gel and leucaena leucocephala seeds with a weight ratio of 6:1 are placed in a drying device, and dehydration drying is carried out under room temperature; dry silica gel processed through 120 DEG C bake-drying and cooling is replaced every day; the ultra-dry seeds are fetched after 5 days; the seeds are sealed in double-layer aluminum foil bags; and the seeds are preserved under room temperature. Before imbibition germination of the preserved ultra-dry leucaena leucocephala seed, a rewetting treatment is carried out, such that the vitality of the ultra-dry seeds is effectively ensured. Before rewetting of the ultra-dry seeds, the seeds are rewetted for 1.5-2.5 days in a drying device with saturated NH4Cl solution in the bottom; the seeds are then rewetted for 1.5-2.5 days in a drying device with water in the bottom; and the seeds are fetched and placed under room temperature. The leucaena leucocephala seed ultra-dry preservation method is simple and feasible, and has the advantage of low investment, low cost of year-round operation, high practicality, and high popularization and application values.
Description
Technical field
The invention belongs to the super dried preservation technical field of seed, relate in particular to a kind of method of super dried preservation white popinac seed.
Background technology
White popinac originates in America, is the perennial shrub of pulse family Mimosoideae Leucaen or arbor, the row self-pollination, but can carry out interspecific cross by artificial pollination, be distributed widely in the torrid zone, subtropical zone.About 800 mutation of white popinac of countries in the world cultivation at present are divided into 3 major types: Hawaii type (plain edition), Salva's many types of (huge kind), Peru's type (medium tree type).Its drought-enduring, anti-lean, adaptability is strong, fast growing and high yield, be of high nutritive value, good palatability, be easy to cultivation, fresh and tender cauline leaf annual production 37～60t/hm
2Gross protein value reaches 22%～29% in the dry matter, also contain rich in amino acid, carotin, multivitamin and trace element, its branches and leaves, beanpod all are the good feeds of ox, sheep, rabbit, chicken, pig happiness food, be described as " miracle tree ", also be described as " the protein warehouse " of arid area by the feed expert of FAO (Food and Agriculture Organization of the United Nation).One of multipurpose fine tree species that to be FAO (Food and Agriculture Organization of the United Nation) promote to the Asian-Pacific area.White popinac serves many purposes: feed, green manure, fuel, forest and phytoremediation, water and soil conservation, cultivation edible mushroom, health care and medicinal etc.Leucaena leaf and spray contain rich in protein, fat, mineral matter and various trace element, are desirable protein feedss, can green grass or young crops raise, driedly raise, ensiling and pasture stock.
Although many to the research of the super kept dry of seed both at home and abroad, not relevant for the research report of the super kept dry of grass seed, only there is couple ultralow temperature (LN in China as yet at present
2,-196 ℃) and the storage time limit of storing grass seed, several gramineous forage grass seed storage characteristic, gramineous forage grass seed reports with the research of vigor relation.White popinac is China torrid zone, the important tropical pasture in south subtropics area, has broad application prospects.With seminal propagation, be the part of tropical pasture germ plasm resource mainly, its seed is the indispensable material base of the good tropical pasture kind of seed selection, is the important materials of carrying out physiologic theory research.Prolong the seed storage life-span to greatest extent, significant for the long preservation of germ plasm resource and high price seed.But, also do not set up the white popinac seed bank at present in the torrid areas, carry out the super dried preservation research of tropical pasture seed, protection, breeding and the germ plasm resource of bio-diversity are preserved with utilizing have very important meaning and application prospect.
Seed moisture content and reserve temperature are two most important factors that influence the seed storage life-span, suitably reduce seed moisture content and reserve temperature and all can effectively prolong seed longevity.But be subjected to the restriction of dry technology and dewatering for a long time, generally believe that stored seeds should not be too dried, classical theory is decided to be seed safety water content lower limit with 5%～7% water content, thinks and breaks through the generation that this lower limit will speed up seed vitality decline and blastation.The condition of the long preservation seed germplasm that FAO (Food and Agriculture Organization of the United Nation) (FAO) and IBPGR (IBPGR) formulate is that the water content of 5%-7% and-18 ℃～-25 ℃ reserve temperature are the general accepted standard in various countries, the world today (IBPGR, 1976).But, the low temperature storage plant germplasm is costly, this is to be difficult to realize for economically less developed region and single breeding units, seed operating unit, and the seed storage technology that develop energy-conservation, economical and convenient and safety is the great practical problem that the preservation plant germplasm resource need solve.
The super dry-storage of seed is the research focus of phase late 1980s germplasm preservation aspect rise, being intended to explore seed moisture content, to be reduced to traditional lower limit following but still effectively keep the dry technology of seed vitality, to reach the purpose of long preservation germ plasm resource under normal temperature condition.Because this technology can reduce the expense of building the storehouse and keeping the storehouse running significantly, have huge economic benefit and application prospect in the germ plasm resource preservation.It preserves technology as energy-saving and environmental protection, economical and practical seed, is subjected to the favor of countries in the world.
Super dry-storage energy significant prolongation seed longevity, storage effect is not second to low temperature storage, 1986, the Ellis of Britain is reduced to 2% with the sesame seed moisture content from 5%, discovery can make its shelf-life prolong more than 40 times, this is equivalent to reserve temperature from 20 ℃ of effects (Ellis and Hong, 1986) that are reduced to-20 ℃.The imagination of super dry storage has been proposed on this basis.In recent years, both at home and abroad super the dried preservation research of seed has been obtained remarkable progress, not only in the physiology of seed field, and has all started no small upsurge in germ plasm resource preservation field, part Study result delivers (Zheng et al., 1998 at " Nature "; Ellis and Roberts, 1998; Demir and Ozcoban, 2007; Perez-Garcia et al., 2007).Compare with low temperature or ultralow temperature storage practice, although the problem that super dry storage exists some to solve technically, this technology is simple and easy to do relatively, small investment, and running expense is low throughout the year, and in environmental protection positive effect is arranged.
There are larger difference in the super dry-storage effect of different monoid seeds, anti-dried degree and super dried time wet condition, and at present, the seed of super dry-storage relates to about more than 50 species, mostly is the seed of vegetables, melon and fruit, beans, cereal, medicinal plant and trees greatly.Studies have shown that most seeds can be down to water content below 5%～7% safely by special dry technologies such as freezing vacuum drying, air blast silica dehydrator, desiccant room temperature dryings, seed storability at normal temperatures increases substantially, in conjunction with specific packing technique, can obtain better preservation effect (Wang Xiaofeng etc., 2001).
People such as Ellis are doing number of research projects aspect the super dry storage of seed, successively the seed of more than 20 kind of kinds such as paddy rice, barley, wheat is tested, the result shows, in certain water content ranges, all all meet seed moisture content and the storage temperature action rule to the seed storage life-span for planting experimentally son, but not of the same race suitable water content lower limit difference (Ellis, 1998; Leon-Lobos and Ellis, 2002; Ellis and Hong, 2006).
The research of the relevant super dry storage of China is started late with respect to Britain.Zheng Guanghua etc. at first carried out the super kept dry research of seed in China in 1989, and were obtaining certain progress (Zheng et al., 1998) aspect the physiology of super dry seed and the cytology.Zheng Guanghua, Cheng Hongyan, Wang Xiaofeng etc. to Chinese cabbage seeds research think, Chinese cabbage seeds through super dried technical finesse, its water content was down to 1.6% o'clock, its seed germination rate and vigor are without any obvious variation, and aspect vitality index and the germination rate burin-in process there is being more patience (Wang et al., 2003) than the contrast seed.People such as Sun Hongmei place 50,35,20 ℃ of storages respectively with the corn seed of different moisture content, and the result shows the optimum moisture content difference of corn seed storage under the different temperatures, presents with the reduction of reserve temperature trend of rising (Sun Hongmei etc., 2004).People's such as Zhou Xiangsheng research thinks that seeds such as the rape of high oil content, radish, Semen sesami nigrum are suitable for super kept dry, but suitable water content lower limit not of the same race variant (Zhou Xiangsheng and Bi Xinhua, 1993).For the imbibition person who solves after super dry handle of seed hinders problem, people such as Zhang Yun orchid carried out different times wet methods to the super dry seeds of crops such as pea, millet in 1994 and have compared, think same variety seeds sometimes its high-moisture seed and low moisture seed adapt to return wet method different, should adopt the suitable wet method of returning according to its seed moisture content height is different.Poly-diethanol (PEG) is returned wet seed, and its concentration transfers influential effect very big to oozing, if improper use does not only have the repairing effect that the accent effect impairs cell membrane on the contrary of oozing.
Seed moisture content and reserve temperature are two most important factors that influence the seed storage life-span, suitably reduce seed moisture content and reserve temperature and all can effectively prolong seed longevity, and the method for preserving seed at present mainly contains:
(1) low temperature or ultralow temperature storage practice, being about to the seed germplasm preserves under the condition of the reserve temperature of 5%～7% water content and-18 ℃～-25 ℃, the low temperature storage plant germplasm is costly, is difficult to realize for economically less developed region and single breeding units, seed operating unit;
(2) super dry storage is energy-conservation, easy, an effective seed storage technology.Although the problem that exists some to solve technically, this technology is simple and easy to do relatively, small investment, and running expense is low throughout the year, and in environmental protection positive effect is arranged.
At present, the seed of super dry-storage relates to about more than 50 species, mostly is the seed of vegetables, melon and fruit, beans, cereal, medicinal plant and trees greatly.Studies have shown that most seeds can be down to water content below 5%～7% safely by special dry technologies such as freezing vacuum drying, air blast silica dehydrator, desiccant room temperature dryings, seed storability at normal temperatures increases substantially, in conjunction with specific packing technique, can obtain better preservation effect.But there are larger difference in the super dry-storage effect of different monoid seeds, anti-dried degree and super dried time wet condition.Therefore, study at the super dry-storage effect of white popinac seed, anti-dried degree and super dried time super dried technology such as wet condition.
Summary of the invention
The invention provides a kind of method of super dried preservation white popinac seed, be intended to simple and easy to do relatively, small investment, running expense is low and have a method of the preservation white popinac seed of positive effect in environmental protection throughout the year.
The object of the present invention is to provide a kind of method of super dried preservation white popinac seed, this method may further comprise the steps:
Step 1 is embedded in the white popinac seed in the drier in the silica gel, at room temperature dehydrates;
Step 2, drier is changed through 120 ℃ of cooled dried silica gel of oven dry every day;
Step 3 is taken out the super dry seeds in the drier after 5 days, will surpass dry seeds and be sealed in room temperature preservation in the double-layer aluminum-foil bag.
Further, in the drier the weight ratio of the silica gel of putting into and white popinac seed be 6: 1.
Further, the long term storage white popinac kind period of the day from 11 p.m. to 1 a.m, the suitableeest super dried water content of the super dry seeds of white popinac is 4 ± 0.2%.
Further, before the white popinac seed imbibition that super dry treatment is preserved was sprouted, returning wet process was the key factor that guarantees super dry seeds vigor.
Further, the super dried implementation method of returning wet process of white popinac seed that super dry treatment is preserved is:
The white popinac seed of super dry treatment preservation is put into the bottom fill saturated NH
4Return wet 2 ± 0.5d in the drier of Cl solution;
Put into drier time wet 2 ± 0.5d that the bottom fills water again, be taken out to room temperature and place.
Further, saturated NH
4Relative moisture in the Cl solution in the drier is 80%RH.
Further, the relative moisture that is filled with water in the drier is 100%RH.
Further, when the white popinac seed surpasses dry treatment, also can at room temperature dehydrate white popinac seed and silica gel are placed in the airtight seed packet.
The method of super dried preservation white popinac seed provided by the invention, be that 6: 1 silica gel and white popinac seed is positioned in the drier with weight ratio, at room temperature dehydrate, change every day through 120 ℃ of cooled dried silica gel of oven dry, take out super dry seeds after 5 days, be sealed in room temperature preservation in the double-layer aluminum-foil bag; Before the white popinac seed imbibition that super dry treatment is preserved is sprouted, return the vigor that wet process can guarantee super dry seeds effectively, super dried the returning when wetting of seed put into seed the bottom earlier and filled saturated NH
4Return wet 2 ± 0.5d in the drier of Cl solution, put into drier time wet 2 ± 0.5d that the bottom fills water then, be taken out to room temperature and place; Should be super dried method of preserving the white popinac seed, simple and easy to do, small investment, long-term running expense are low, practical, have stronger popularization and using value.
Description of drawings
Fig. 1 is the super dried realization flow figure that preserves the method for white popinac seed that the embodiment of the invention provides;
Fig. 2 is the super dried realization flow figure that returns the method for wet process of the white popinac seed to super dry treatment preservation that the embodiment of the invention provides.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further described in detail.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in and limit invention.
The super dried realization flow of preserving the method for white popinac seed that Fig. 1 provides for the embodiment of the invention.
This method may further comprise the steps:
In step S101, the white popinac seed is embedded in the drier in the silica gel, dehydrate under the room temperature;
In step S102, drier is changed through 120 ℃ of cooled dried silica gel of oven dry every day;
In step S103, take out the super dry seeds in the drier after 5 days, will surpass thousand seeds and be sealed in room temperature preservation in the double-layer aluminum-foil bag.
In embodiments of the present invention, in the drier the weight ratio of the silica gel of putting into and white popinac seed be 6: 1.
In embodiments of the present invention, the long term storage white popinac kind period of the day from 11 p.m. to 1 a.m, the suitableeest super dried water content of the super dry seeds of white popinac is 4 ± 0.2%.
In embodiments of the present invention, before the white popinac seed imbibition that super dry treatment is preserved was sprouted, returning wet process was the key factor that guarantees super dry seeds vigor.
The super dried implementation method flow process of returning wet process of the white popinac seed to super dry treatment preservation that Fig. 2 provides for the embodiment of the invention.
In embodiments of the present invention, the super dried implementation method of returning wet process of white popinac seed that super dry treatment is preserved is:
In step S201, the white popinac seed of super dry treatment preservation is put into the bottom fill saturated NH
4Return wet 2 ± 0.5d in the drier of Cl solution;
In step S202, put into drier time wet 2 ± 0.5d that the bottom fills water again, be taken out to room temperature and place.
In embodiments of the present invention, saturated NH
4Relative moisture in the Cl solution in the drier is 80%RH.
In embodiments of the present invention, the relative moisture in the drier that is filled with water is 100%RH.
In embodiments of the present invention, when the white popinac seed surpasses dry treatment, also can at room temperature dehydrate white popinac seed and silica gel are placed in the airtight seed packet.
Below in conjunction with drawings and the specific embodiments application principle of the present invention is further described.
Experiment material:
(1) vegetable material: different leaf white popinac, water content 7.28%, germination rate 82.7%;
(2) chemicals: silica gel, ammonium chloride;
(3) instrument and equipment: illumination box, high constant temperature oven, biochemical incubator, aluminium foil bag, drier, cracker;
Test method: obtaining and preserving of (1) super dry seeds, adopt the seed of the super dry treatment of silica dehydrator method to place seed packet, be embedded in the interior silica gel of drier.Silica gel is 6: 1 with the seed weight ratio, at room temperature dehydrates.Change every day through 120 ℃ of cooled dried silica gel of oven dry.Regularly weigh every day, with the super dry seeds of preparation different moisture content.Super dry seeds is sealed in the double-layer aluminum-foil bag.
(2) seed moisture content is measured, and measures (State Bureau of Technical Supervision, 2001) according to " grass seed inspection procedure ".Adopt high constant temperature oven method, get seed and grind, take by weighing sample 6.000～7.000g with 1/1000 analytical balance, repeat twice, dry 2h under 130 ℃～133 ℃ conditions is chilled to room temperature, weighs.
(3) percentage of seedgermination and vitality test are with 80 ℃ of hot water seed soaking 3～5min, after 70% ethanol surface sterilization, fully rinse well with distilled water, place the two layers of filter paper bed to germinate then, place 28 ℃ of illumination boxs to germinate, 100 in every part of seed, triplicate.Keep the skin wet every day, adds up germination vigor behind the 4d, adds up germination rate behind the 10d.Vitality index (VI) is calculated as follows: VI=GI * Sx, germination index GI=∑ (Gt/Dt) wherein, the germinative number of Gt:t after day; Dt: germination number of days; Sx: the seedling of germination 4d long (get 10 strains and measure its mean value) (international seed testing association, 1993).
(4) seed returns wet process, before the testing index, will surpass dry seeds earlier and put into the bottom and fill saturated NH
4Return wet 50h in the drier of Cl solution, put into the drier time wet 50h that the bottom fills water then, be taken out to room temperature and place.
Method step:
The first step: measure different leaf white popinac seed moisture content, germination rate and germination index and record, room temperature preservation is in seed;
Second step: seed is placed seed packet, and silica gel is 6: 1 with the seed weight ratio, is embedded in the interior silica gel of drier, at room temperature dehydrates.Change every day through 120 ℃ of cooled dried silica gel of oven dry.Take out super dry seeds (the suitableeest super dried water content is (4 ± 0.2) %) after 5 days, be sealed in room temperature preservation in the double-layer aluminum-foil bag.Measure seed moisture content, germination rate and germination index and record;
The 3rd step: take out an amount of super dry seeds, other seeds still are sealed in room temperature preservation in the double-layer aluminum-foil bag, will surpass dry seeds and fill saturated NH bottom putting into
4Return wet 50h in the drier of Cl solution, put into the drier time wet 50h that the bottom fills water then, be taken out to room temperature and place;
The 4th step: measure experimental data and records such as seed moisture content, germination rate and germination index.Use in order to other experiment.
The beneficial effect that technical solution of the present invention is brought: super dry treatment is to the influence of different leaf white popinac seed vitality, and result of the test shows, when seed without returning wet process, water content was down to 0.58% o'clock by 7.28%, seed vitality descends with water content and significantly reduces.Super dry seeds is returned wet process before imbibition is sprouted, when water content is not less than 4.12%, potentiality of seed 60.7%, germination rate 82.5%, germination index 33.76, vitality index 57.39 all with contrast no significant difference.But when water content drops to 4.12% when following, potentiality of seed, germination rate, germination index, vitality index all with contrast difference significantly (table 1), show and return the physics repair efficiency that wet process can reach plasma membrane in advance, thereby improved the activity level of super dry seeds.4.12% water content is the critical moisture content that super dry seeds is preserved vigor, and super dry treatment is harmless to different leaf white popinac seed, and returns the key factor that wet process is the super dry seeds vigor of assurance before the imbibition sprouting.The suitableeest super dried water content is 4 ± 0.2%.
The super dry seeds vigor of the different leaf white popinac of table 1 different moisture content changes
The ageing resistance of super dried white popinac seed:
The white popinac seed is through 50 ℃ of burin-in process 0～15d, and the germination rate of super dry seeds and vitality index keep stable with the increase of aging fate, and contrast seed germination rate and vitality index significantly descend.The aging 5d of contrast seed moisture content 7.28%, germination rate and vitality index sharply are down to 65.6% and 31.76 from 82.7% and 57.47, and 5～15d slowly is down to 54.3% and 22.53 subsequently; And water content be 4.12% super dry seeds behind burin-in process 15d, germination rate and vitality index and 0d contrast indifference, stable remaining on 81.7% and 56.81 the level.
The super dry-storage of white popinac seed is to the influence of vigor:
The room temperature storage of different leaf white popinac seed is after 2 years, and seed vitality descends very fast, and seed moisture content is down to 4.12%, then demonstrates stronger long keeping ability.As seen from Table 2, at room temperature preserve 2 years through the white popinac of super dry treatment after, germination rate, seedling grow and index such as seed vitality on still keep higher level, compare with-20 ℃ of stored seedses and significant difference not occur with 5 ℃.The contrast seed is under similarity condition, and indexs such as its germination rate, germination index, seedling length and vitality index reduce significantly, compares with the low temperature storage seed with super dry seeds to present significant difference, and seed has produced serious bad change.
The comparison of table 2 storage different leaf white popinac seed vitality after 2 years
Annotate: different letter representation significant differences (P＜0.01).
Super dry seeds preparation method: silica gel is 6: 1 with the seed weight ratio, is embedded in the interior silica gel of drier, at room temperature dehydrates.Change every day through 120 ℃ of cooled dried silica gel of oven dry.Take out super dry seeds (the suitableeest super dried water content is 4 ± 0.2%) after 5 days, be sealed in room temperature preservation in the double-layer aluminum-foil bag.
The super dried wet method of returning of seed: earlier seed is put into the bottom and filled saturated NH
4Return wet 2 ± 0.5d in the drier of Cl solution (80%RH), put into the bottom then and fill water (100%RH)) drier return wet 2 ± 0.5d, be taken out to room temperature and place.
The method of the super dried preservation white popinac seed that the embodiment of the invention provides, be that 6: 1 silica gel and white popinac seed is positioned in the drier with weight ratio, at room temperature dehydrate, change every day through 120 ℃ of cooled dried silica gel of oven dry, take out super dry seeds after 5 days, be sealed in room temperature preservation in the double-layer aluminum-foil bag; Before the white popinac seed imbibition that super dry treatment is preserved is sprouted, return the vigor that wet process can guarantee super dry seeds effectively, super dried the returning when wetting of seed put into seed the bottom earlier and filled saturated NH
4Return wet 2 ± 0.5d in the drier of Cl solution, put into drier time wet 2 ± 0.5d that the bottom fills water then, be taken out to room temperature and place; Should be super dried method of preserving the white popinac seed, simple and easy to do, small investment, long-term running expense are low, practical, have stronger popularization and using value.
The above only is preferred embodiment of the present invention, not in order to limiting the present invention, all any modifications of doing within the spirit and principles in the present invention, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. the method for a super dried preservation white popinac seed is characterized in that this method may further comprise the steps:
Step 1 is embedded in the white popinac seed in the drier in the silica gel, at room temperature dehydrates;
Step 2, drier is changed through 120 ℃ of cooled dried silica gel of oven dry every day;
Step 3 is taken out the super dry seeds in the drier after 5 days, will surpass dry seeds and be sealed in room temperature preservation in the double-layer aluminum-foil bag.
2. the method for claim 1 is characterized in that, in the drier the weight ratio of the silica gel of putting into and white popinac seed be 6: 1.
3. the method for claim 1 is characterized in that, the long term storage white popinac kind period of the day from 11 p.m. to 1 a.m, and the suitableeest super dried water content of the super dry seeds of white popinac is 4 ± 0.2%.
4. the method for claim 1 is characterized in that, before the white popinac seed imbibition that super dry treatment is preserved was sprouted, returning wet process was the key factor that guarantees super dry seeds vigor.
5. method as claimed in claim 4 is characterized in that, the super dried implementation method of returning wet process of white popinac seed that super dry treatment is preserved is:
The white popinac seed of super dry treatment preservation is put into the bottom fill saturated NH
4Return wet 2 ± 0.5d in the drier of Cl solution; Put into drier time wet 2 ± 0.5d that the bottom fills water again, be taken out to room temperature and place.
6. method as claimed in claim 5 is characterized in that, saturated NH
4Relative moisture in the Cl solution in the drier is 80%RH.
7. method as claimed in claim 5 is characterized in that, the relative moisture in the drier that is filled with water is 100%RH.
8. the method for claim 1 is characterized in that, when the white popinac seed surpasses dry treatment, also can at room temperature dehydrate white popinac seed and silica gel are placed in the airtight seed packet.
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| CN105850988A (en) * | 2016-06-15 | 2016-08-17 | 山东农业大学 | Maize germplasm storage cup |
| CN109907171A (en) * | 2019-03-12 | 2019-06-21 | 云南省草地动物科学研究院 | A kind of ensiling processing and utilization method of white popinac |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105123354A (en) * | 2015-08-31 | 2015-12-09 | 三峡大学 | Method for biologically preventing and controlling leucaena glauca diffusion |
| CN105123354B (en) * | 2015-08-31 | 2017-10-27 | 三峡大学 | A kind of method of biological control white popinac diffusion |
| CN105850988A (en) * | 2016-06-15 | 2016-08-17 | 山东农业大学 | Maize germplasm storage cup |
| CN105850988B (en) * | 2016-06-15 | 2019-01-15 | 山东农业大学 | Corn germ plasm resource reservoir cups |
| CN109907171A (en) * | 2019-03-12 | 2019-06-21 | 云南省草地动物科学研究院 | A kind of ensiling processing and utilization method of white popinac |
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