CN105475271B - A kind of method of artemisia annua seed long-term preservation - Google Patents
A kind of method of artemisia annua seed long-term preservation Download PDFInfo
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- CN105475271B CN105475271B CN201511002432.7A CN201511002432A CN105475271B CN 105475271 B CN105475271 B CN 105475271B CN 201511002432 A CN201511002432 A CN 201511002432A CN 105475271 B CN105475271 B CN 105475271B
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- 235000001405 Artemisia annua Nutrition 0.000 title claims abstract description 80
- 240000000011 Artemisia annua Species 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000004321 preservation Methods 0.000 title claims abstract description 19
- 230000007774 longterm Effects 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 241000196324 Embryophyta Species 0.000 claims abstract description 24
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 15
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000006013 carbendazim Substances 0.000 claims abstract description 15
- 238000001035 drying Methods 0.000 claims abstract description 8
- 238000001291 vacuum drying Methods 0.000 claims abstract description 8
- 238000004806 packaging method and process Methods 0.000 claims abstract description 3
- 239000000843 powder Substances 0.000 claims description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 17
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 10
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 10
- 239000004571 lime Substances 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 6
- 239000005030 aluminium foil Substances 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- 230000008774 maternal effect Effects 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 238000009423 ventilation Methods 0.000 claims description 5
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 4
- 239000003292 glue Substances 0.000 claims description 4
- 239000004563 wettable powder Substances 0.000 claims description 4
- 230000000717 retained effect Effects 0.000 claims 1
- 239000004575 stone Substances 0.000 claims 1
- 108010016634 Seed Storage Proteins Proteins 0.000 abstract description 8
- 238000011161 development Methods 0.000 abstract description 6
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 206010013786 Dry skin Diseases 0.000 abstract 1
- 239000011888 foil Substances 0.000 abstract 1
- 238000004064 recycling Methods 0.000 abstract 1
- BLUAFEHZUWYNDE-NNWCWBAJSA-N artemisinin Chemical compound C([C@](OO1)(C)O2)C[C@H]3[C@H](C)CC[C@@H]4[C@@]31[C@@H]2OC(=O)[C@@H]4C BLUAFEHZUWYNDE-NNWCWBAJSA-N 0.000 description 7
- 229930101531 artemisinin Natural products 0.000 description 6
- 240000001851 Artemisia dracunculus Species 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 230000035784 germination Effects 0.000 description 5
- 229940079593 drug Drugs 0.000 description 4
- 201000004792 malaria Diseases 0.000 description 4
- 229930183339 qinghaosu Natural products 0.000 description 4
- 239000003430 antimalarial agent Substances 0.000 description 3
- 229960004191 artemisinin Drugs 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000282994 Cervidae Species 0.000 description 2
- 241000723353 Chrysanthemum Species 0.000 description 2
- 235000007516 Chrysanthemum Nutrition 0.000 description 2
- 230000000078 anti-malarial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- 229910021532 Calcite Inorganic materials 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 244000000013 helminth Species 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to Seed storage field, specially a kind of method of artemisia annua seed long-term preservation, this method includes several steps：Step 1: the selection of plant left to run to seed；Step 2: the collection of seed；Step 3: Seeds preprocess：First with carbendazim sprinkling sterilization 13 minutes, quick-frozen the surface of the seed water sublimate is taken out after recycling limewash 25 hours stifling thereafter；Step 4: vacuum drying：Using 4 DEG C of dryings of vacuum drying chamber temperature adjustment, water content stops up to 7% 8%；Step 5: packaging：It is packed using double-layer aluminum-foil valve bag, while pouring nitrogen-sealed preservation in bag；Step 6: storage：Packaged artemisia annua seed is put into 24 DEG C of conditions to store.Method provided by the invention extends the shelf-life of artemisia annua seed, ensure that artemisia annua variety and quality, the sustainable development for artemisia annua industry provides technical support.
Description
Technical field
The present invention relates to Seed storage fields, the especially method of artemisia annua seed long-term preservation.
Background technology
Artemisia annua (sweet wormwood) Artemisia annua L. are traditional Chinese medicine, and China medical personal is the beginning of the seventies from Huang
A kind of novel antimalarial ingredient-qinghaosu is found in flower wormwood artemisia, is had to brain type malaria, malignant malaria, tertian fever etc. efficient, quick-acting, low
The feature of poison, preferred antimalarial agent was appointed as in 2004 by the World Health Organization.And qinghaosu finder slaughter cry of a deer in
In September, 2011 obtains the Lars gram prize for being known as the Nobel Prize " wind vane ", slaughter within 2015 cry of a deer obtain the Nobel Prize, this is
The world-class highest Grand Prix that China's natural science circle obtains so far.Malaria is popular most wide, incidence in the world today
And dead highest tropical parasitic worm infectious disease.Global malaria differentiation is distributed in more than 100 country, clinical patients about 4-5 hundred million according to statistics
People has ten thousand people of 200-300 death every year, wherein the most serious with Africa and Hispanic country.Artemisinin-based drug is one
Kind of political drug, President Hu Jintao resist cruel drug as support artemisine on " in non-summit meeting " in 2006
One of the 4 big key projects in Africa, especially written instructions support qinghaosu industry.In the Eleventh Five-Year Plan implemented in 2006, China
Government provides 300,000,000 RMB and gives African country for purchasing artemisinin-based drug.Artemisia annua is qinghaosu unique raw material plant,
China is the major production areas of artemisia annua, and the raw material in the world 90% comes from China, and artemisia annua is made that not in world's antimalarial history
Appreciable contribution.
Restrict at present artemisia annua is high-quality, high yield, sustainable development factor mainly there are two aspects：1. wormwood artemisia is strictly to be selfed
Not affine plant, selfing rate is less than 5%, and artemisia annua quality deterioration is more serious after hybridization, and qinghaosu contains between different single plants
It is also very big to measure difference, so the selection and breeding of artemisia annua high quality seed are more difficult；2. conventional artemisia annua Seed storage method, seed are lived
Power is reduced than very fast, and the holding time is soon.It is difficult for artemisia annua seed breeding, the Seed storage time soon the technical issues of, this
A kind of high-quality method of artemisia annua seed long-term preservation of invention offer, and experiments indicate that, it is processed by the present invention
Novel species percentage of seedgermination up to the germination percentage about 70% of seed after 99%, 4 year, the germination percentage of 3 years kinds is 76% or so.Institute
It is to ensure the effective way of artemisia annua germplasm quality, but yet there are no related Huang with the long-term preservation of artemisia annua high quality seed
The report of flower wormwood artemisia seed long-term preservation.This patent provides a kind of method of artemisia annua long-term preservation, be artemisia annua is high-quality, high yield,
Sustainable development provides reference frame.
Invention content
The present invention is intended to provide a kind of method of long-term preservation artemisia annua high quality seed, extends guaranteeing the quality for artemisia annua seed
Phase ensure that artemisia annua variety and quality, and the sustainable development for artemisia annua industry provides technical support, in order to make up the prior art
Deficiency, provide a kind of method of artemisia annua seed long-term preservation, specific technical solution is as follows：
A kind of method of artemisia annua seed long-term preservation, this method mainly include the following steps that：
1), the selection of plant left to run to seed：Select plant and maternal variation little, and robust growth, artemislnin content are high
Plant；
2), the collection of seed：Artemisia annua seed is completely smoothed out with the fingers down from plant left to run to seed, is then placed in ventilation and shady place
It spontaneously dries, cannot be protected in sieve by being taken again with 40 mesh sieve sieve after the impurity of sieve followed by the removal of 24 mesh sieve
The seed stayed is the artemisia annua seed that can be reserved seed for planting；
3), Seeds preprocess：The sprinkling processing of artemisia annua seed is sterilized for 1-3 minutes with carbendazim first, uses pearl
Artemisia annua seed is placed under room temperature and impregnates 5-10 minutes by layer amidin, clean with clear water after immersion, again with limewash every
Water smoking steams 2-5 hours, and limewash mass percent concentration is 1%-4%, takes out seed and passes through accelerated freeze-drying, the surface of the seed has one
Layer lime moisture film, stops；
4) it, is dried in vacuo：It will shakeout in pallet, be put into vacuum drying chamber by pretreated artemisia annua seed,
Temperature is adjusted to 3-5 DEG C and is dried, and the time is 2-4 days, stops drying when finally water content being examined to reach 7%-8%；
5) it, packs：Dried artemisia annua seed is packed into double-deck self-styled aluminium foil bag, while being passed through nitrogen, it is last close
Envelope；
6) it, is stored：It is 2-4 DEG C of repository that intact artemisia annua seed, which will be sealed, and be put into temperature, is preserved.
Preferably, in step 2) seed collection：Acquisition time is annual early November or mid-November.
Preferably, the proportioning of carbendazim is 50% wettable powder in step 3), and 40% glue is outstanding, surplus by water be made
Carbendazim solution.
Preferably, nacreous layer powder aqueous solution is that nacreous layer powder that weight component ratio is 1: 30-50 and water stir in step 3)
It mixes.
Preferably, during Seeds preprocess limewash water proof is stifling in step 3), lime coolant-temperature gage is controlled at 30-40 DEG C,
Seed is with lime water level mutually from 6-8cm, and process can not stir.
Preferably, in step 5) seed packaging：The unsuitable overfill of seed pack, it should which there are 1/4 spaces, are filled with nitrogen
Gas.
Preferably, the intact artemisia annua seed that will seal in step 6) is put into repository, the artemisia annua seed often wrapped
Sack can not stack mutually from 0.5-1cm.
Beneficial effects of the present invention：The present invention strictly controls the water of Seed storage from seed selection, pretreatment during dry etc.
Point and temperature.Sterilization processing is carried out to seed with carbendazim first, ensures that seed is not corroded during preserving by bacterium；Its
Secondary cooperation nacreous layer powder solution impregnates, and the composed structure of shell is divided into three layers, outermost cuticula, the prism shell of middle layer
With internal layer nacre, nacre is made of corner post shape calcite, and the nacre that nacreous layer powder is then shell internal layer scrapes off mill
At powder, compared to pearl powder containing abundant metallic element, trace element and amino acid, and its water-soluble and absorbability pole
It is high.Seed nutritional ingredient after being impregnated by nacreous layer powder increases, and the isolation by boiling layer powder and lime stone after stifling
Moisture content causes the Free water of seed to reduce；It is impregnated by nacreous layer powder and lime moisture film wraps up seed, on the one hand solve seed
It is on the other hand also solved so as to cause the phenomenon of seed necrosis in the suspend mode for preserving the pathogen destruction seed that will appear in the process
Determined Seed storage initial stage have faint breath effect generate moisture drying problem.It finally combines the selection of drying temperature and contains
The control of water allows seed to inhibit the growth of bacterium well, and moisture is few, and nutritional ingredient consumption is few, and last metabolism is slow,
To achieve the purpose that effectively to improve survival rate, the long-term preservation of seed.Method provided by the invention extends artemisia annua seed
Shelf-life, ensure that artemisia annua variety and quality, the sustainable development for artemisia annua industry provides technical support.It yet there are no
Report in relation to artemisia annua seed long-term preservation, method provided by the invention have very vast potential for future development.
Specific implementation mode
To keep the object, technical solutions and advantages of the present invention more clear, this is invented with reference to examples of implementation
It is described in further detail.
Embodiment 1
1), the selection of plant left to run to seed：Select plant and maternal variation little, and robust growth, artemislnin content are high
Plant；
2), the collection of seed：Artemisia annua seed is completely smoothed out with the fingers down from plant left to run to seed, is then placed in ventilation and shady place
It spontaneously dries, cannot be protected in sieve by being taken again with 40 mesh sieve sieve after the impurity of sieve followed by the removal of 24 mesh sieve
The seed stayed is the artemisia annua seed that can be reserved seed for planting；
3), Seeds preprocess：The sprinkling processing of artemisia annua seed is sterilized for 1 minute with carbendazim first, uses nacre
Artemisia annua seed is placed under room temperature by amidin to be impregnated 5 minutes, is cleaned with clear water after immersion, stifling with limewash water proof again
2 hours, limewash mass percent concentration was 1%, takes out seed and passes through accelerated freeze-drying, and the surface of the seed has one layer of lime moisture film,
Stop；
4) it, is dried in vacuo：It will shakeout in pallet, be put into vacuum drying chamber by pretreated artemisia annua seed,
Temperature is adjusted to 3 DEG C and is dried, and the time is 2 days, stops drying when finally water content being examined to reach 7%；
5) it, packs：Dried artemisia annua seed is packed into double-deck self-styled aluminium foil bag, while being passed through nitrogen, it is last close
Envelope；
6) it, is stored：It is 2 DEG C of repositories that intact artemisia annua seed, which will be sealed, and be put into temperature, is preserved.It handles well
Seed under conditions of 4 DEG C, water content 7.2%, up to 4 years holding time, germination percentage 60%.
Embodiment 2
1), the selection of plant left to run to seed：Select plant and maternal variation little, and robust growth, artemislnin content are high
Plant；
2), the collection of seed：Artemisia annua seed is completely smoothed out with the fingers down from plant left to run to seed, is then placed in ventilation and shady place
It spontaneously dries, cannot be protected in sieve by being taken again with 40 mesh sieve sieve after the impurity of sieve followed by the removal of 24 mesh sieve
The seed stayed is the artemisia annua seed that can be reserved seed for planting；
3), Seeds preprocess：The sprinkling processing of artemisia annua seed is sterilized for 1 minute with carbendazim first, carbendazim
Proportioning is 50% wettable powder, and 40% glue is outstanding, and carbendazim solution is made in the water that surplus is, with nacreous layer powder aqueous solution by chrysanthemum
Wormwood artemisia seed, which is placed under room temperature, to be impregnated 5 minutes, and nacreous layer powder aqueous solution is the nacreous layer powder that weight component ratio is 1: 30 and water stirring
It forms, is cleaned with clear water after immersion, 2 hours stifling with limewash water proof again, limewash mass percent concentration is 1%, is taken
Going out seed and passes through accelerated freeze-drying, the surface of the seed has one layer of lime moisture film, stops, during limewash water proof is stifling, lime coolant-temperature gage control
System is at 30 DEG C, and seed is with lime water level phase from 6cm, and process can not stir；
4) it, is dried in vacuo：It will shakeout in pallet, be put into vacuum drying chamber by pretreated artemisia annua seed,
Temperature is adjusted to 3 DEG C and is dried, and the time is 2 days, stops drying when finally water content being examined to reach 7%；
5) it, packs：Dried artemisia annua seed is packed into double-deck self-styled aluminium foil bag, while being passed through nitrogen, it is last close
Envelope, the unsuitable overfill of seed pack, it should which there are 1/4 spaces, are filled with nitrogen；
6) it, is stored：It is 2 DEG C of repositories that intact artemisia annua seed, which will be sealed, and be put into temperature, is preserved, will be sealed
Intact artemisia annua seed is put into repository, and the artemisia annua seed sack often wrapped can not stack mutually from 0.5cm.
The seed handled well is under conditions of 4 DEG C, water content 7.2%, up to 5 years holding time, germination percentage 65%
Embodiment 3
1), the selection of plant left to run to seed：Select plant and maternal variation little, and robust growth, artemislnin content are high
Plant；
2), the collection of seed：Artemisia annua seed is completely smoothed out with the fingers down from plant left to run to seed, is then placed in ventilation and shady place
It spontaneously dries, cannot be protected in sieve by being taken again with 40 mesh sieve sieve after the impurity of sieve followed by the removal of 24 mesh sieve
The seed stayed is the artemisia annua seed that can be reserved seed for planting；
3), Seeds preprocess：The sprinkling processing of artemisia annua seed is sterilized for 3 minutes with carbendazim first, carbendazim
Proportioning is 50% wettable powder, and 40% glue is outstanding, and carbendazim solution is made in the water that surplus is, with nacreous layer powder aqueous solution by chrysanthemum
Wormwood artemisia seed, which is placed under room temperature, to be impregnated 10 minutes, and nacreous layer powder aqueous solution is the nacreous layer powder that weight component ratio is 1: 50 and water stirring
It forms, is cleaned with clear water after immersion, 5 hours stifling with limewash water proof again, limewash mass percent concentration is 4%, is taken
Going out seed and passes through accelerated freeze-drying, the surface of the seed has one layer of lime moisture film, stops, during limewash water proof is stifling, lime coolant-temperature gage control
System is at 40 DEG C, and seed is with lime water level phase from 8cm, and process can not stir；
4) it, is dried in vacuo：It will shakeout in pallet, be put into vacuum drying chamber by pretreated artemisia annua seed,
Temperature is adjusted to 3 DEG C and is dried, and the time is 2 days, stops drying when finally water content being examined to reach 7%；
5) it, packs：Dried artemisia annua seed is packed into double-deck self-styled aluminium foil bag, while being passed through nitrogen, it is last close
Envelope, the unsuitable overfill of seed pack, it should which there are 1/4 spaces, are filled with nitrogen；
6) it, is stored：It is 4 DEG C of repositories that intact artemisia annua seed, which will be sealed, and be put into temperature, is preserved, will be sealed
Intact artemisia annua seed is put into repository, and the artemisia annua seed sack often wrapped can not stack mutually from 1cm.It handles well
Seed is under conditions of 4 DEG C, water content 7.2%, up to 5 years holding time, germination percentage 68%.
Table 1 is the influence of different moisture content to Seed storage at 25 DEG C of embodiment 2
Table 2 is the influence that embodiment 2 preserves Seed storage in different temperatures
Seed moisture content 7.2%
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (5)
1. a kind of method of artemisia annua seed long-term preservation, which is characterized in that the method includes following steps:
1), the selection of plant left to run to seed：Select plant and maternal variation little, and robust growth, the high plant of artemislnin content；
2）, seed collection：Artemisia annua seed is completely smoothed out with the fingers down from plant left to run to seed, ventilation is then placed in and shady place is natural
It is dry, it cannot be retained in sieve by being taken again with 40 mesh sieve sieve after the impurity of sieve followed by the removal of 24 mesh sieve
The seed come is the artemisia annua seed that can be reserved seed for planting；
3）, Seeds preprocess：The sprinkling processing of artemisia annua seed is sterilized for 1-3 minutes with carbendazim first, uses nacreous layer powder
Artemisia annua seed is placed under room temperature by aqueous solution to be impregnated 5-10 minutes, is cleaned with clear water after immersion, smoked with limewash water proof again
It steams 2-5 hours, limewash mass percent concentration is 1%-4%, takes out seed and passes through accelerated freeze-drying, the surface of the seed has one layer of lime
Moisture film stops；The proportioning of the carbendazim is 50% wettable powder, and 40% glue is outstanding, and carbendazim solution is made in the water of surplus；It is described
Nacreous layer powder aqueous solution is that weight component ratio is 1:The nacreous layer powder and water of 30-50 stirs；
4）, vacuum drying：It will shakeout in pallet, be put into vacuum drying chamber, temperature by pretreated artemisia annua seed
It is adjusted to 3-5 DEG C to be dried, the time is 2-4 days, stops drying when finally water content being examined to reach 7%-8%；
5）, packaging：Dried artemisia annua seed is packed into double-deck self-styled aluminium foil bag, while being passed through nitrogen, finally sealed；
6）, stored：It is 2-4 DEG C of repository that intact artemisia annua seed, which will be sealed, and be put into temperature, is preserved.
2. the method for artemisia annua seed long-term preservation according to claim 1, which is characterized in that step 2）The receipts of middle seed
Collection：Acquisition time is annual early November or mid-November.
3. the method for artemisia annua seed long-term preservation according to claim 1, which is characterized in that step 3）Middle seed is located in advance
During reason stone buck water proof is stifling, the control of lime coolant-temperature gage is at 30-40 DEG C, and seed and lime water level phase are from 6-8cm, and process
It can not stir.
4. the method for artemisia annua seed long-term preservation according to claim 1, which is characterized in that step 5）The packet of middle seed
Dress：The unsuitable overfill of seed pack, it should which there are 1/4 spaces, are filled with nitrogen.
5. the method for artemisia annua seed long-term preservation according to claim 1, which is characterized in that step 6）In will seal
Good artemisia annua seed is put into repository, and the artemisia annua seed sack often wrapped can not stack mutually from 0.5-1cm.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6641827B2 (en) * | 1998-08-21 | 2003-11-04 | Takeda Chemical Industries, Ltd. | Insecticide obtained from plants for use on termites |
| CN101444158A (en) * | 2008-12-23 | 2009-06-03 | 重庆市中药研究院 | Method for storing seeds of southernwood |
| CN104206267A (en) * | 2014-09-18 | 2014-12-17 | 李国桥 | Hybridization breeding method of traditional Chinese medicinal material-sweet wormwood |
| CN104429558A (en) * | 2014-12-22 | 2015-03-25 | 中国林业科学研究院亚热带林业研究所 | Seed field seedling growing method for sapindus mukorossi gaertn |
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2015
- 2015-12-28 CN CN201511002432.7A patent/CN105475271B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6641827B2 (en) * | 1998-08-21 | 2003-11-04 | Takeda Chemical Industries, Ltd. | Insecticide obtained from plants for use on termites |
| CN101444158A (en) * | 2008-12-23 | 2009-06-03 | 重庆市中药研究院 | Method for storing seeds of southernwood |
| CN104206267A (en) * | 2014-09-18 | 2014-12-17 | 李国桥 | Hybridization breeding method of traditional Chinese medicinal material-sweet wormwood |
| CN104429558A (en) * | 2014-12-22 | 2015-03-25 | 中国林业科学研究院亚热带林业研究所 | Seed field seedling growing method for sapindus mukorossi gaertn |
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