CN105462961A - 一种提取核酸的方法及试剂盒 - Google Patents
一种提取核酸的方法及试剂盒 Download PDFInfo
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- CN105462961A CN105462961A CN201610023898.3A CN201610023898A CN105462961A CN 105462961 A CN105462961 A CN 105462961A CN 201610023898 A CN201610023898 A CN 201610023898A CN 105462961 A CN105462961 A CN 105462961A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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Abstract
Description
0.2ng组 | 0天 | 1天 | 2天 | 5天 | 10天 | 20天 |
0.1%TritonX-100 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 |
ddH2O | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 |
0.1ng组 | 0天 | 1天 | 2天 | 5天 | 10天 | 20天 |
0.1%TritonX-100 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 |
ddH2O | 4/4 | 4/4 | 4/4 | 4/4 | 3/4 | 3/4 |
0.05ng组 | 0天 | 1天 | 2天 | 5天 | 10天 | 20天 |
0.1%TritonX-100 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 |
ddH2O | 4/4 | 4/4 | 4/4 | 4/4 | 2/4 | 0/4 |
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CN105462961B CN105462961B (zh) | 2019-04-19 |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107459480A (zh) * | 2017-09-05 | 2017-12-12 | 镇江斯格派医疗器械有限公司 | 一种双芳基脲类组蛋白去乙酰化酶抑制剂 |
CN108251414A (zh) * | 2018-01-17 | 2018-07-06 | 云健康基因科技(上海)有限公司 | 骨髓细胞基因组dna的提取方法及提取试剂盒 |
CN108864278A (zh) * | 2018-07-27 | 2018-11-23 | 珠海宝锐生物科技有限公司 | 一种制备分子生物级牛血清白蛋白的方法 |
CN108893465A (zh) * | 2018-07-25 | 2018-11-27 | 古蔺县公安局 | 磁珠用于显现手印痕迹的用途以及手印痕迹dna提取方法 |
CN112322614A (zh) * | 2020-11-16 | 2021-02-05 | 武汉吉诺百客医学科技有限公司 | 一种磁珠法提取血液基因组dna的试剂盒及其使用方法 |
CN112899132A (zh) * | 2021-01-13 | 2021-06-04 | 上海金鑫生物科技有限公司 | 一种用于核酸提取与纯化的硅胶过滤吸头 |
CN112921028A (zh) * | 2019-12-06 | 2021-06-08 | 深圳华大基因科技服务有限公司 | Dna纯化方法、基因组dna的提取方法、测序方法和试剂盒 |
CN113046417A (zh) * | 2021-03-25 | 2021-06-29 | 武汉吉诺百客医学科技有限公司 | 一种磁珠法提取血浆游离dna的试剂盒及其使用方法 |
CN113755484A (zh) * | 2020-06-01 | 2021-12-07 | 苏州新海生物科技股份有限公司 | 一种核酸提取方法以及应用 |
Citations (3)
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CN1539013A (zh) * | 2001-07-09 | 2004-10-20 | ������������ʽ���� | 应用非织造织物纯化和检测核酸的方法 |
CN101613697B (zh) * | 2009-08-05 | 2011-06-15 | 公安部物证鉴定中心 | 一种提取纯化dna的方法 |
CN102725406A (zh) * | 2009-12-29 | 2012-10-10 | 通用电气医疗集团生物科学公司 | 核酸洗脱的改进 |
-
2016
- 2016-01-14 CN CN201610023898.3A patent/CN105462961B/zh active Active
Patent Citations (3)
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---|---|---|---|---|
CN1539013A (zh) * | 2001-07-09 | 2004-10-20 | ������������ʽ���� | 应用非织造织物纯化和检测核酸的方法 |
CN101613697B (zh) * | 2009-08-05 | 2011-06-15 | 公安部物证鉴定中心 | 一种提取纯化dna的方法 |
CN102725406A (zh) * | 2009-12-29 | 2012-10-10 | 通用电气医疗集团生物科学公司 | 核酸洗脱的改进 |
Non-Patent Citations (2)
Title |
---|
徐文莉等: "《磁珠法核酸提取对检测低HBV DNA病毒载量的临床价值》", 《热带医学杂志》 * |
查瑶等: "《煮沸裂解法与磁珠法在HBV DNA荧光定量检测中的核酸提取效果》", 《浙江预防医学》 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107459480A (zh) * | 2017-09-05 | 2017-12-12 | 镇江斯格派医疗器械有限公司 | 一种双芳基脲类组蛋白去乙酰化酶抑制剂 |
CN108251414A (zh) * | 2018-01-17 | 2018-07-06 | 云健康基因科技(上海)有限公司 | 骨髓细胞基因组dna的提取方法及提取试剂盒 |
CN108893465A (zh) * | 2018-07-25 | 2018-11-27 | 古蔺县公安局 | 磁珠用于显现手印痕迹的用途以及手印痕迹dna提取方法 |
CN108864278A (zh) * | 2018-07-27 | 2018-11-23 | 珠海宝锐生物科技有限公司 | 一种制备分子生物级牛血清白蛋白的方法 |
CN108864278B (zh) * | 2018-07-27 | 2021-02-19 | 珠海宝锐生物科技有限公司 | 一种制备分子生物级牛血清白蛋白的方法 |
CN112921028A (zh) * | 2019-12-06 | 2021-06-08 | 深圳华大基因科技服务有限公司 | Dna纯化方法、基因组dna的提取方法、测序方法和试剂盒 |
CN113755484A (zh) * | 2020-06-01 | 2021-12-07 | 苏州新海生物科技股份有限公司 | 一种核酸提取方法以及应用 |
CN112322614A (zh) * | 2020-11-16 | 2021-02-05 | 武汉吉诺百客医学科技有限公司 | 一种磁珠法提取血液基因组dna的试剂盒及其使用方法 |
CN112899132A (zh) * | 2021-01-13 | 2021-06-04 | 上海金鑫生物科技有限公司 | 一种用于核酸提取与纯化的硅胶过滤吸头 |
CN113046417A (zh) * | 2021-03-25 | 2021-06-29 | 武汉吉诺百客医学科技有限公司 | 一种磁珠法提取血浆游离dna的试剂盒及其使用方法 |
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Inventor after: Li Min Inventor after: Zhou Ruhua Inventor after: Shi Yunjie Inventor after: Zhang Jian Inventor after: Wang Xin Inventor after: Zhang Wei Inventor after: Li Pengfei Inventor after: Bi Wanli Inventor after: Wang Zhifeng Inventor before: Bi Wanli Inventor before: Zhou Ruhua Inventor before: Wang Zhifeng Inventor before: Shi Yunjie Inventor before: Zhang Jian Inventor before: Zhang Wei Inventor before: Li Pengfei |
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