CN105461451A - Microbial fermentation water-soluble fertilizer and preparation method thereof - Google Patents
Microbial fermentation water-soluble fertilizer and preparation method thereof Download PDFInfo
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- CN105461451A CN105461451A CN201510984792.5A CN201510984792A CN105461451A CN 105461451 A CN105461451 A CN 105461451A CN 201510984792 A CN201510984792 A CN 201510984792A CN 105461451 A CN105461451 A CN 105461451A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention provides a microbial fermentation water-soluble fertilizer and a preparation method thereof. The water-soluble fertilizer is prepared from the following raw materials: 70 to 100 parts of compound amino acid powder, 900 to 950 parts of yeast waste liquid, 10 to 30 parts of a dispersant, 50 to 100 parts of a chelated mid-element fertilizer, 2 to 5 parts of a chelated microelement fertilizer, 1 to 3 parts of a pichia pastoris fermentation agent and 1 to 3 parts of a bacillus subtillis fermentation agent. The method comprises the following steps: (1) mixing the compound amino acid powder and the yeast waste liquid; after adjusting the pH, filtering; (2) inoculating pichia pastoris and bacillus subtillis and fermenting for 5 to 7 days; (3) mixing the dispersant, the chelated mid-element fertilizer, the chelated microelement fertilizer and fermentation liquid. The microbial fermentation water-soluble fertilizer provided by the invention has the effects of improving soil, retaining fertilizer and water, stimulating the growth of crops, promoting disease prevention and insect resistance of the crops, increasing both production and income, improving the quality of agricultural products and the like.
Description
Technical field
The present invention relates to technical field of microbial fermentation, be specifically related to a kind of fermentable Water soluble fertilizer and preparation method thereof.
Background technology
China is a large agricultural country, and along with the fast development of agricultural, liquid manure resource consumption is on the rise, and also impacts environment, and therefore water saving joint is fertile is of great significance realizing China's agricultural sustainable development tool.Current the Integration of Water into Fertilizer can fundamentally address this problem, and not only achieves high-efficiency agriculture, and has saved water resources, improves chemical utilization rate, reduces chemical fertilizer to the pollution of environment.
Water soluble fertilizer (abbreviation Water soluble fertilizer) is a kind of new high-efficiency fertilizer that can be completely water-soluble, have dissolving well, without residue, instant effect, the remarkable advantage such as easy to use, be convenient to management and the popularization of the Integration of Water into Fertilizer, obtain at home in recent years and develop rapidly.
Summary of the invention
The object of the invention is to realize high-efficiency agriculture, saving water resource, improving chemical utilization rate, reduce chemical fertilizer to the pollution of environment, and a kind of novel fermentable Water soluble fertilizer and preparation method thereof is provided.Water soluble fertilizer of the present invention is rich in pre-biotic microorganism, humic acid, amino acid and moderate-element, be particularly suitable for the cash crop such as field crop and gourd, fruit and vegetable, can meet crop to moderate-element demand while, there is improvement soil, fertilizer conservation water conservation, stimulate plant growth, promote crop disease prevention anti-evil, improve the effects such as utilization rate of fertilizer, increasing both production and income, improvement quality of agricultural product.
The invention provides following technical proposals to solve the problems of the technologies described above.
One of technical scheme provided by the invention is: a kind of fermentable Water soluble fertilizer, it comprises following feed composition: with parts by weight, compound amino acid powder 70 ~ 100 parts, yeast waste liquor 900 ~ 950 parts, dispersion agent 10 ~ 30 parts, chelating secondary nutrients 50 ~ 100 parts, 2 ~ 5 parts, chelated microelement fertilizer, Pichia yeast (Pichiapastoris) starter 1 ~ 3 part, Bacillus subtillis (Bacillussubtills) starter 1 ~ 3 part, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.
Wherein, described compound amino acid powder is for described in the routine of this area, and it is the directed concentrated extract of effective amino-acid nutritive medium.In general, it is for raw material forms through hydrolysis, depickling, desalination and spraying dry with natural keratin hair, containing abundant Free amino acid, the elements such as organic and inorganic nitrogen, phosphorus, potassium also containing crop needs, general commercially available compound amino acid powder is all applicable to the present invention.Preferably, described compound amino acid powder contains L-glutamic acid, Histidine, aspartic acid, proline(Pro), α-amino-isovaleric acid, L-Ala, leucine, Methionin, arginine, Serine, tryptophane, methionine(Met), phenylalanine, tyrosine, glycine and Threonine.
Described yeast waste liquor (also known as yeast concentrated solution) is for described in the routine of this area, and it is the waste liquid produced in Yeast Fermentation Process.Generally speaking, it contains organic matter 45% ~ 55%, N+P
2o
5+ K
2o total amount is greater than 4.0%, moisture 40% ~ 45%, pH value 7.1 ~ 8.0.
Described dispersion agent is for described in the routine of this area, main play make system evenly, suspension property increase, non-setting effect, conventional dispersion agent such as Macrogol 200, poly(oxyethylene glycol) 400 and ethylene-acrylic acid copolymer etc. are all applicable to the present invention, the preferred poly(oxyethylene glycol) 400 of the present invention.
Described chelating secondary nutrients is the implication of this area routine, obtains with chelant ties moderate-element by fertilizer, and described sequestrant is that this area is conventional, sequestrant as conventional in EDTA, IDHA, HEDP etc.Described moderate-element is the implication of this area routine, preferably, described moderate-element be selected from calcium, magnesium and sulphur one or more.
Described chelated microelement fertilizer is the implication of this area routine, and obtain with chelant ties trace element by fertilizer, described sequestrant is that this area is conventional, sequestrant as conventional in EDTA, IDHA, HEDP etc.Described trace element is the implication of this area routine, preferably, described trace element be selected from copper, manganese, zinc and iron one or more.
Described Pichia yeast (Pichiapastoris) starter is for described in the routine of this area, as this area is conventional, its be generally by Pichia yeast bacterial classification through overactivation expand numerous obtain seed liquor (preferred secondary seed solution) after re-use, be added into fermentation system by seed liquor and ferment.
Described Bacillus subtillis (Bacillussubtills) starter is for described in the routine of this area, as this area is conventional, its be generally by Bacillus subtillis bacterial classification through overactivation expand numerous obtain seed liquor (preferred secondary seed solution) after re-use, be added into fermentation system by seed liquor and ferment.
The consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the implication of the weighing scale of bacterium liquid inoculated refer to that these two kinds of starters addition manner is in use not limited to bacterium liquid and (is preferably seed liquor, more preferably secondary seed solution), also can be the mode of other this area routines, no matter but which kind of addition manner, can be such as add solid-state bacterium powder, its addition be all equivalent to add and the OD of bacterium liquid with bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.
A preferred embodiments of the present invention is: a kind of fermentable Water soluble fertilizer, it comprises following feed composition: with parts by weight, compound amino acid powder 90 parts, yeast waste liquor 900 parts, dispersion agent 30 parts, chelating secondary nutrients 100 parts, 5 parts, chelated microelement fertilizer, pichia spp bacterium leavening agent 2 parts, Bacillus subtillis starter 2 parts, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.
Another preferred embodiments of the present invention is: a kind of fermentable Water soluble fertilizer, it comprises following feed composition: with parts by weight, compound amino acid powder 70 parts, yeast waste liquor 950 parts, dispersion agent 30 parts, chelating secondary nutrients 100 parts, 5 parts, chelated microelement fertilizer, pichia spp bacterium leavening agent 3 parts, Bacillus subtillis starter 3 parts, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.
Two of technical scheme provided by the invention is: the preparation method of aforementioned micro organism fermentation Water soluble fertilizer, it comprises the steps:
(1) compound amino acid powder is mixed with yeast waste liquor, between adjust ph to 6.5 ~ 7.5, mixed solution is filtered, obtains filtered liquid;
(2) in filtered liquid, inoculate pichia spp bacterium leavening agent and Bacillus subtillis starter, ferment 5 ~ 7 days to obtain fermented liquid;
(3) dispersion agent, chelating secondary nutrients, chelated microelement fertilizer are mixed with fermented liquid, to obtain final product.
In the present invention, step (1) is: mixed with yeast waste liquor by compound amino acid powder, between adjust ph to 6.5 ~ 7.5, is filtered by mixed solution, obtains filtered liquid.
Wherein, described adjust ph is this area routine operation, preferably for using NaOH/HCl solution adjust ph, more preferably uses the NaOH/HCl solution adjust ph of 6mol/L.
Describedly be filtered into this area routine, it does not have particular requirement in concrete operations, and the mechanical filter technique of this area routine is all applicable to the present invention.
In step (1) after described filtered liquid, preferably filtered liquid is sent into the fermentation operation of carrying out step (2) in bio-reactor.
Correspondingly, the fermentation described in step (2) is preferably carried out in bio-reactor.Preferably, the condition of described fermentation is: leavening temperature 25 DEG C ~ 30 DEG C, mixing speed 150 ~ 200r/min, air flow keep 1.5 ~ 1.8VVM.If fermentation is not carried out at bio-reactors such as fermentor tanks, its reaction conditions can be adjusted accordingly, but basic suitable with aforementioned condition.
The technical indicator of fermentable Water soluble fertilizer of the present invention: pichia spp (Pichiapastoris), its cell concentration is 1.0 × 10
8~ 5.0 × 10
8cfu/mL; Bacillus subtillis (Bacillussubtills), its cell concentration is 1.0 × 10
8~ 5.0 × 10
8cfu/mL; Content of humic acid 250 ~ 300g/L; Aminoacids content 70 ~ 100g/L; Middle amount essence nutrient content 50 ~ 80g/L; Micronutrient content 2 ~ 5g/L.
Fermentable Water soluble fertilizer of the present invention contains pre-biotic microorganism, amino acid, humic acid and moderate-element, crop yield can be increased, improve the ability that crop disease-resistant harms, improve quality of agricultural product, both to have fostered and apply fertilizer soil fertility, additionally reducing environmental pollution, is first-selected fertilizer that is green, non-polluted farm product.Moderate-element, trace element are that crop growth must obligato nutritive ingredient, not only can improve output, and to improving the quality of agricultural-food clearly, also effectively can strengthen the resistance of plant for disease, low temperature, high temperature and arid; Humic acid in Water soluble fertilizer has improvement soil physico-chemical property, promotes that soil agreegate is formed, native saltiness is shown in reduction, improves soil exchange capacity, reduces the potential of hydrogen of saline-alkali soil, promotes the functions such as seed roots; Pre-biotic microorganism is colonizated in edatope, its growth and breeding and Metabolic activity make Organic Matter Transformation form soil ulmin, add soil fertilizer water retention capacity, and these pre-biotic microorganism can also secrete the disease-resistant material of some growth-promotings, improve the resistance of crop, reduce disease; Inorganic carbonate radical ion, phosphate anion that chelant ties moderate-element, trace element avoid in moderate-element, trace element and soil form not easily molten carbonate and phosphoric acid salt, improve the utilization ratio of secondary nutrients, and amino acid self directly can be absorbed by plant, stimulating plant grows.
On the basis meeting this area general knowledge, above-mentioned each optimum condition, can arbitrary combination, obtains the preferred embodiments of the invention.
Agents useful for same of the present invention and raw material are all commercially.
Positive progressive effect of the present invention is:
Fermentable Water soluble fertilizer provided by the invention, utilize pre-biotic microorganism by yeast waste liquor and aminoacid mixture for raw material carries out microbe conversion for humic acid, and add chelating moderate-element, chelated microelement, dispersion agent, form the water soluble fertilizer being rich in pre-biotic microorganism, humic acid, amino acid, chelating moderate-element and chelated microelement, there is improvement soil, fertilizer conservation water conservation, stimulate plant growth, promote the anti-evil of crop disease prevention, improve the advantages such as utilization rate of fertilizer, increasing both production and income, improvement quality of agricultural product.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.In following each embodiment, unless otherwise noted, being conventional commercial can obtain for the various kinds of equipment used, reagent and material.
Wherein, chelating secondary nutrients is calcium carbonate, calcium sulfate, magnesium sulfate;
Chelated microelement fertilizer is copper sulfate, zinc sulfate, manganous sulfate, ferrous sulfate;
Pichia yeast (Pichiapastoris), Bacillus subtillis (Bacillussubtills) are all purchased from Beijing Voto Sky & Land Biotech Co., Ltd..
The production of embodiment 1 fermentable Water soluble fertilizer
1, composition of raw materials: with parts by weight, compound amino acid powder 80 parts, yeast waste liquor 900 parts, dispersion agent 30 parts, chelating secondary nutrients 80 parts, 5 parts, chelated microelement fertilizer, Pichia yeast (Pichiapastoris) starter 1 part, Bacillus subtillis (Bacillussubtills) starter 2.5 parts, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.Wherein, L-glutamic acid, Histidine, aspartic acid, proline(Pro), α-amino-isovaleric acid, L-Ala, leucine, Methionin, arginine, Serine, tryptophane, methionine(Met), phenylalanine, tyrosine, glycine and Threonine is contained in compound amino acid powder; Moderate-element comprises calcium, sulphur, magnesium; Trace element comprises zinc, copper, iron; Dispersion agent is ethylene-acrylic acid copolymer.
2, preparation method:
(1) compound amino acid powder is mixed with yeast waste liquor, by the NaOH/HCl solution adjust ph to 6.5 of 6mol/L, by mixed solution mechanical filter, obtain filtered liquid, filtered liquid is sent into fermentor tank;
(2) in filtered liquid, inoculate Pichia yeast and Bacillus subtillis is fermented 5 days, the condition of fermentation is: leavening temperature 30 DEG C, mixing speed 180r/min, air flow keep 1.5VVM;
(3) dispersion agent, chelating secondary nutrients, chelated microelement fertilizer are mixed with fermented liquid, to obtain final product.
3, technical target of the product:
After measured, contain in finished product Water soluble fertilizer:
The production of embodiment 2 fermentable Water soluble fertilizer
1, composition of raw materials: with parts by weight, compound amino acid powder 100 parts, yeast waste liquor 900 parts, dispersion agent 30 parts, chelating secondary nutrients 50 parts, 2 parts, chelated microelement fertilizer, Pichia yeast (Pichiapastoris) starter 3 parts, Bacillus subtillis (Bacillussubtills) starter 1 part, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.Wherein, L-glutamic acid, Histidine, aspartic acid, proline(Pro), α-amino-isovaleric acid, L-Ala, leucine, Methionin, arginine, Serine, tryptophane, methionine(Met), phenylalanine, tyrosine, glycine and Threonine is contained in compound amino acid powder; Moderate-element comprises calcium, magnesium and sulphur; Trace element comprises copper, manganese, zinc and iron; Dispersion agent is Macrogol 200.
2, preparation method:
(1) compound amino acid powder is mixed with yeast waste liquor, by the NaOH/HCl solution adjust ph to 7.5 of 6mol/L, by mixed solution mechanical filter, obtain filtered liquid, filtered liquid is sent into fermentor tank;
(2) in filtered liquid, inoculate Pichia yeast and Bacillus subtillis is fermented 7 days, the condition of fermentation is: leavening temperature 28 DEG C, mixing speed 150r/min, air flow keep 1.6VVM;
(3) dispersion agent, chelating secondary nutrients, chelated microelement fertilizer are mixed with fermented liquid, to obtain final product.
3, technical target of the product:
After measured, contain in finished product Water soluble fertilizer:
The production of embodiment 3 fermentable Water soluble fertilizer
1, composition of raw materials: with parts by weight, compound amino acid powder 100 parts, yeast waste liquor 920 parts, dispersion agent 10 parts, chelating secondary nutrients 50 parts, 2 parts, chelated microelement fertilizer, Pichia yeast (Pichiapastoris) starter 1 part, Bacillus subtillis (Bacillussubtills) starter 1 part, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.Wherein, L-glutamic acid, Histidine, aspartic acid, proline(Pro), α-amino-isovaleric acid, L-Ala, leucine, Methionin, arginine, Serine, tryptophane, methionine(Met), phenylalanine, tyrosine, glycine and Threonine is contained in compound amino acid powder; Moderate-element comprises calcium, magnesium and sulphur; Trace element comprises copper, manganese, zinc and iron; Dispersion agent is poly(oxyethylene glycol) 400.
2, preparation method:
(1) compound amino acid powder is mixed with yeast waste liquor, by the NaOH/HCl solution adjust ph to 7.0 of 6mol/L, by mixed solution mechanical filter, obtain filtered liquid, filtered liquid is sent into fermentor tank;
(2) in filtered liquid, inoculate Pichia yeast and Bacillus subtillis is fermented 6 days, the condition of fermentation is: leavening temperature 28 DEG C, mixing speed 200r/min, air flow keep 1.5VVM;
(3) dispersion agent, chelating secondary nutrients, chelated microelement fertilizer are mixed with fermented liquid, to obtain final product.
3, technical target of the product:
After measured, contain in finished product Water soluble fertilizer:
The production of embodiment 4 fermentable Water soluble fertilizer
1, composition of raw materials: with parts by weight, compound amino acid powder 90 parts, yeast waste liquor 900 parts, dispersion agent 30 parts, chelating secondary nutrients 100 parts, 5 parts, chelated microelement fertilizer, Pichia yeast (Pichiapastoris) starter 2 parts, Bacillus subtillis (Bacillussubtills) starter 2 parts, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.Wherein, L-glutamic acid, Histidine, aspartic acid, proline(Pro), α-amino-isovaleric acid, L-Ala, leucine, Methionin, arginine, Serine, tryptophane, methionine(Met), phenylalanine, tyrosine, glycine and Threonine is contained in compound amino acid powder; Moderate-element comprises calcium, magnesium and sulphur; Trace element comprises copper, manganese, zinc and iron; Dispersion agent is poly(oxyethylene glycol) 400.
2, preparation method:
(1) compound amino acid powder is mixed with yeast waste liquor, by the NaOH/HCl solution adjust ph to 6.8 of 6mol/L, by mixed solution mechanical filter, obtain filtered liquid, filtered liquid is sent into fermentor tank;
(2) in filtered liquid, inoculate Pichia yeast and Bacillus subtillis is fermented 6 days, the condition of fermentation is: leavening temperature 25 DEG C, mixing speed 180r/min, air flow keep 1.6VVM;
(3) dispersion agent, chelating secondary nutrients, chelated microelement fertilizer are mixed with fermented liquid, to obtain final product.
3, technical target of the product:
After measured, contain in finished product Water soluble fertilizer:
The production of embodiment 5 fermentable Water soluble fertilizer
1, composition of raw materials: with parts by weight, compound amino acid powder 70 parts, yeast waste liquor 950 parts, dispersion agent 30 parts, chelating secondary nutrients 100 parts, 5 parts, chelated microelement fertilizer, Pichia yeast (Pichiapastoris) starter 3 parts, Bacillus subtillis (Bacillussubtills) starter 3 parts, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.Wherein, L-glutamic acid, Histidine, aspartic acid, proline(Pro), α-amino-isovaleric acid, L-Ala, leucine, Methionin, arginine, Serine, tryptophane, methionine(Met), phenylalanine, tyrosine, glycine and Threonine is contained in compound amino acid powder; Moderate-element comprises calcium, magnesium and sulphur; Trace element comprises copper, manganese, zinc and iron; Dispersion agent is poly(oxyethylene glycol) 400.
2, preparation method:
(1) compound amino acid powder is mixed with yeast waste liquor, by the NaOH/HCl solution adjust ph to 7.0 of 6mol/L, by mixed solution mechanical filter, obtain filtered liquid, filtered liquid is sent into fermentor tank;
(2) in filtered liquid, inoculate Pichia yeast and Bacillus subtillis is fermented 6 days, the condition of fermentation is: leavening temperature 28 DEG C, mixing speed 170r/min, air flow keep 1.8VVM;
(3) dispersion agent, chelating secondary nutrients, chelated microelement fertilizer are mixed with fermented liquid, to obtain final product.
3, technical target of the product:
After measured, contain in finished product Water soluble fertilizer:
The application and result of effect example fermentable Water soluble fertilizer
Fermentable Water soluble fertilizer prepared by the present invention is applicable to crop time of infertility, mainly as topdressing.Fertilizing method can be selected drip irrigation according to local irrigation conditions and crop tillage method, sprays or rush with water fertilization modes such as executing.Rate of fertilizer application can change to some extent according to fertilization mode, agrotype and the difference of breeding time, and generally, punching is executed and filled with root 5 ~ 10kg/ mu, uses, monthly use 1 ~ 2 time after being watered 1000 ~ 1500 times of dilutions; If adopt drip irrigation and foliage-spray, use after dilute with water 200 ~ 500 times.
The booth vegetable of fermentable Water soluble fertilizer on Beijing, Hebei, Shandong, Yunnan and other places embodiment 1 ~ 5 produced, tomato, celery, strawberry, capsicum and food crop have done contrast demonstration test, test-results shows: fermentable Water soluble fertilizer not only the effect of increasing income is remarkable, and disease and insect resistance and anti-continuous cropping effect also fairly obvious, crop products quality is also very excellent.
The experiment effect that the fermentable Water soluble fertilizer that embodiment 1 ~ 5 obtains is used for celery is as shown in table 1:
Table 1 Water soluble fertilizer celery test-results
As can be seen from Table 1, use fermentable Water soluble fertilizer to compare with not applying fertilizer with use chemical fertilizer, crop per mu yield all increases a lot, than average volume increase more than 30% of not applying fertilizer.
The experiment effect that the fermentable Water soluble fertilizer that embodiment 1 ~ 5 obtains is used for tomato is as shown in table 2:
Table 2 Water soluble fertilizer tomato test-results
As can be seen from Table 2, use fermentable Water soluble fertilizer of the present invention, output can be made significantly to promote, and Tomato Quality is obviously improved.Use fermentable Water soluble fertilizer of the present invention, tomato per mu yield improves more than 54.1% than not applying fertilizer, and improves more than 20% than using chemical fertilizer; Tomato Vc content improves more than 7.41mg/kg than not applying fertilizer, and improves more than 4.55mg/kg than using chemical fertilizer.Such effect has been improve greatly and improve in this area, has unexpected part.
The experiment effect that the fermentable Water soluble fertilizer that embodiment 1 ~ 5 obtains is used for strawberry is as shown in table 3:
Table 3 Water soluble fertilizer strawberry test-results
As can be seen from Table 3, strawberry cultivating uses fermentable Water soluble fertilizer of the present invention than not applying fertilizer, apply the yield and quality of chemical fertilizer is all significantly increased: apply fermentable Water soluble fertilizer of the present invention than not applying fertilizer output increased more than 17.2%, in fruit, Vc content exceeds more than 2.2mg/10g, and total Soluble Sugar exceeds more than 31.7g/kg; Apply fermentable Water soluble fertilizer of the present invention than the output increased more than 5.9% applying chemical fertilizer, in fruit, Vc content exceeds more than 0.9mg/10g, and total Soluble Sugar exceeds more than 13.1g/kg.Analyze from the output of greenhouse strawberry demonstration plant, quality and Financial cost, use fermentable Water soluble fertilizer product of the present invention can significantly improve the economic benefit of strawberry.
Although above with general explanation, embodiment and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. a fermentable Water soluble fertilizer, it is characterized in that, it comprises following feed composition: with parts by weight, compound amino acid powder 70 ~ 100 parts, yeast waste liquor 900 ~ 950 parts, dispersion agent 10 ~ 30 parts, chelating secondary nutrients 50 ~ 100 parts, 2 ~ 5 parts, chelated microelement fertilizer, Pichia yeast (Pichiapastoris) starter 1 ~ 3 part, Bacillus subtillis (Bacillussubtills) starter 1 ~ 3 part, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.
2. fermentable Water soluble fertilizer according to claim 1, it is characterized in that, described compound amino acid powder contains L-glutamic acid, Histidine, aspartic acid, proline(Pro), α-amino-isovaleric acid, L-Ala, leucine, Methionin, arginine, Serine, tryptophane, methionine(Met), phenylalanine, tyrosine, glycine and Threonine.
3. fermentable Water soluble fertilizer according to claim 1, is characterized in that, described dispersion agent be selected from Macrogol 200, poly(oxyethylene glycol) 400 and ethylene-acrylic acid copolymer one or more.
4. fermentable Water soluble fertilizer according to claim 1, is characterized in that, described moderate-element be selected from calcium, magnesium and sulphur one or more.
5. fermentable Water soluble fertilizer according to claim 1, is characterized in that, described trace element be selected from copper, manganese, zinc and iron one or more.
6. fermentable Water soluble fertilizer according to claim 1, it is characterized in that, it comprises following feed composition: with parts by weight, compound amino acid powder 90 parts, yeast waste liquor 900 parts, dispersion agent 30 parts, chelating secondary nutrients 100 parts, 5 parts, chelated microelement fertilizer, pichia spp bacterium leavening agent 2 parts, Bacillus subtillis starter 2 parts, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.
7. fermentable Water soluble fertilizer according to claim 1, it is characterized in that, it comprises following feed composition: with parts by weight, compound amino acid powder 70 parts, yeast waste liquor 950 parts, dispersion agent 30 parts, chelating secondary nutrients 100 parts, 5 parts, chelated microelement fertilizer, pichia spp bacterium leavening agent 3 parts, Bacillus subtillis starter 3 parts, the consumption of described pichia spp bacterium leavening agent and described fermentation of bacillus subtilis agent is all with when starter is for bacterium liquid and the OD of bacterium liquid
600value for 3 ~ 4 time the weighing scale of bacterium liquid inoculated.
8. the preparation method of fermentable Water soluble fertilizer described in any one of claim 1 ~ 7, it comprises the steps:
(1) compound amino acid powder is mixed with yeast waste liquor, between adjust ph to 6.5 ~ 7.5, mixed solution is filtered, obtains filtered liquid;
(2) in filtered liquid, inoculate pichia spp bacterium leavening agent and Bacillus subtillis starter, ferment 5 ~ 7 days to obtain fermented liquid;
(3) dispersion agent, chelating secondary nutrients, chelated microelement fertilizer are mixed with fermented liquid, to obtain final product.
9. preparation method according to claim 8, is characterized in that, in step (1), described adjust ph, for using NaOH/HCl solution adjust ph, more preferably uses the NaOH/HCl solution adjust ph of 6mol/L; In step (1) after described filtered liquid, filtered liquid is sent into the fermentation operation of carrying out step (2) in bio-reactor; Correspondingly, the fermentation described in step (2) is carried out in bio-reactor.
10. preparation method according to claim 9, is characterized in that, the condition of described fermentation is: leavening temperature 25 DEG C ~ 30 DEG C, mixing speed 150 ~ 200r/min, air flow keep 1.5 ~ 1.8VVM.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN108395359A (en) * | 2018-06-06 | 2018-08-14 | 陕西鼎天济农腐殖酸制品有限公司 | A kind of high concentration humic acid applying liquid fertilizer and its production method |
| CN109369268A (en) * | 2018-11-26 | 2019-02-22 | 沈阳禾康永丰生物环保产业有限公司 | The production method of amino acid Water soluble fertilizer |
| CN109553473A (en) * | 2018-12-26 | 2019-04-02 | 龙蟒大地农业有限公司 | A kind of Water soluble fertilizer and preparation method thereof |
| CN109608259A (en) * | 2018-12-26 | 2019-04-12 | 龙蟒大地农业有限公司 | One kind is rich in biostimulant substance Water soluble fertilizer and preparation method thereof |
| CN110128214A (en) * | 2019-06-19 | 2019-08-16 | 乐斯福(明光)有限公司 | A method of it is produced using yeast thick slurry waste liquid containing humic acid water-soluble fertilizer |
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| CN112441860A (en) * | 2020-12-10 | 2021-03-05 | 潍坊福若海生物技术有限公司 | Water-soluble fertilizer synergistic composition and preparation method and application thereof |
| CN111302857B (en) * | 2020-02-26 | 2022-07-05 | 新疆农垦科学院 | Water-soluble organic biological bacterial fertilizer for drip irrigation tomatoes and preparation method thereof |
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| CN106518290B (en) * | 2016-10-09 | 2019-07-23 | 长沙学院 | Tailored version plant nutrient agent and preparation method thereof for producing high-quality tomato |
| CN106518290A (en) * | 2016-10-09 | 2017-03-22 | 长沙学院 | Special plant nutritional agent for producing high quality tomatoes and preparation method thereof |
| JPWO2019172277A1 (en) * | 2018-03-05 | 2021-03-11 | 味の素株式会社 | Composition for inducing disease resistance of plants or controlling disease of plants |
| WO2019172277A1 (en) * | 2018-03-05 | 2019-09-12 | 味の素株式会社 | Composition for inducing plant disease damage resistance or preventing plant disease damage |
| JP7375743B2 (en) | 2018-03-05 | 2023-11-08 | 味の素株式会社 | Composition for inducing plant disease resistance or controlling plant diseases |
| CN108395359A (en) * | 2018-06-06 | 2018-08-14 | 陕西鼎天济农腐殖酸制品有限公司 | A kind of high concentration humic acid applying liquid fertilizer and its production method |
| CN109369268A (en) * | 2018-11-26 | 2019-02-22 | 沈阳禾康永丰生物环保产业有限公司 | The production method of amino acid Water soluble fertilizer |
| CN109553473A (en) * | 2018-12-26 | 2019-04-02 | 龙蟒大地农业有限公司 | A kind of Water soluble fertilizer and preparation method thereof |
| CN109608259A (en) * | 2018-12-26 | 2019-04-12 | 龙蟒大地农业有限公司 | One kind is rich in biostimulant substance Water soluble fertilizer and preparation method thereof |
| CN110128214A (en) * | 2019-06-19 | 2019-08-16 | 乐斯福(明光)有限公司 | A method of it is produced using yeast thick slurry waste liquid containing humic acid water-soluble fertilizer |
| CN110541012A (en) * | 2019-08-26 | 2019-12-06 | 福建三炬生物科技股份有限公司 | preparation method of amino acid liquid, amino acid water-soluble fertilizer and application thereof |
| CN110963848A (en) * | 2019-12-30 | 2020-04-07 | 杨金铎 | Functional composite bacterial fertilizer and preparation method thereof |
| CN111302857B (en) * | 2020-02-26 | 2022-07-05 | 新疆农垦科学院 | Water-soluble organic biological bacterial fertilizer for drip irrigation tomatoes and preparation method thereof |
| CN112441860A (en) * | 2020-12-10 | 2021-03-05 | 潍坊福若海生物技术有限公司 | Water-soluble fertilizer synergistic composition and preparation method and application thereof |
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