CN105432513A - Method for artificial propagation of longnose gudgeon - Google Patents
Method for artificial propagation of longnose gudgeon Download PDFInfo
- Publication number
- CN105432513A CN105432513A CN201510703792.3A CN201510703792A CN105432513A CN 105432513 A CN105432513 A CN 105432513A CN 201510703792 A CN201510703792 A CN 201510703792A CN 105432513 A CN105432513 A CN 105432513A
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- parent
- minnow
- longnose
- gudgeon
- female
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 241001125877 Gobio gobio Species 0.000 title 1
- 241000251468 Actinopterygii Species 0.000 claims abstract description 17
- 239000007924 injection Substances 0.000 claims abstract description 14
- 238000002347 injection Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 13
- 230000002196 ecbolic effect Effects 0.000 claims abstract description 10
- 239000003814 drug Substances 0.000 claims abstract description 8
- 229940079593 drug Drugs 0.000 claims abstract description 8
- 239000004570 mortar (masonry) Substances 0.000 claims abstract description 4
- 210000000006 pectoral fin Anatomy 0.000 claims abstract description 4
- 241000594009 Phoxinus phoxinus Species 0.000 claims description 48
- 230000004720 fertilization Effects 0.000 claims description 13
- 230000032696 parturition Effects 0.000 claims description 12
- 102000002322 Egg Proteins Human genes 0.000 claims description 8
- 108010000912 Egg Proteins Proteins 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 210000004681 ovum Anatomy 0.000 claims description 8
- 241000270295 Serpentes Species 0.000 claims description 7
- 241000252233 Cyprinus carpio Species 0.000 claims description 5
- 206010062767 Hypophysitis Diseases 0.000 claims description 5
- 210000004556 brain Anatomy 0.000 claims description 5
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical class C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 claims description 5
- 239000002434 gonadorelin derivative Substances 0.000 claims description 5
- 239000000890 drug combination Substances 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 210000002149 gonad Anatomy 0.000 claims description 2
- 230000006978 adaptation Effects 0.000 claims 1
- 230000000638 stimulation Effects 0.000 claims 1
- 241001125879 Gobio Species 0.000 abstract 6
- 210000001550 testis Anatomy 0.000 abstract 3
- 239000000243 solution Substances 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 239000004744 fabric Substances 0.000 abstract 1
- DPWSRXJWCYEGIV-CWMZTGDLSA-N acetic acid;n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-[(2s)-2-(ethylcarbamoyl)pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxoprop Chemical compound CC(O)=O.CC(O)=O.CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 DPWSRXJWCYEGIV-CWMZTGDLSA-N 0.000 description 5
- 235000013601 eggs Nutrition 0.000 description 5
- 244000144987 brood Species 0.000 description 4
- 230000008774 maternal effect Effects 0.000 description 3
- 230000013011 mating Effects 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 208000037805 labour Diseases 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 241000239250 Copepoda Species 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 241000252206 Cypriniformes Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000643938 Saurogobio dabryi Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention discloses a method for artificial propagation of longnose gudgeon. The method comprises the steps of: A, after a mature longnose gudgeon parent is fished out of water, placing the parent into an indoor spawning pond; B, when propagation is started, fishing out the longnose gudgeon parent, injecting an oxytocic drug after fixing the longnose gudgeon parent with a hand, wherein a pectoral fin base portion injection is adopted; C, injecting an oxytocic solution into each female parent, injecting an oxytocic solution into a male parent, and placing the injected parents back into the spawning pond; D, when the effect time is reached, after the female fish is fished out from the spawning pond, wiping the body surface with dry cloth, and extruding spawn into a dry plastic basin; E, acquiring fish sperms, removing a testis of the male fish, and placing the testis on a filter-paper to remove moisture, then placing the testis in a mortar to grind; and F, adopting artificial fecundation, wherein normal saline is used for fecundation, the ratio of male and female is 10:1. The method is easy to implement and easy to operate, and has a good effect of propagation; the problem of artificial propagation of longnose gudgeon is effectively solved, so that the propagation rate of longnose gudgeon can reach about 85%, and the fecundation rate is up to more than 80%.
Description
Technical field
The present invention relates to aquaculture field, particularly relate to the induced spawning method of a kind of She Minnow, it is applicable to the artificial propagation that She Minnow belongs to other kind of fish.
Background technology
She Minnow (Saurogobiodabryi) is under the jurisdiction of Cypriniformes, Cyprinidae, Minnow subfamily, She Minnow belongs to, and is a kind of small-sized economic fish, widely distributed in China, to the south until each water system of the Zhujiang River has She Minnow distributes from Heilungkiang.She Minnow is middle lower floor small fishes, with aquatic insect and copepoda for Major Foods.She Minnow mating season is the 3-5 month, ovum tool microviscosity, ovum footpath 1.0-1.1mm.She Minnow dressing percentage is high, stings little between flesh, and meat fertilizer is tender, delicious flavour, and nutritive value is higher, and the market price is high, demand is large.She Minnow is as the special breed variety of local name, and the exploitation being subject to the concern ， She Minnow industry of government and scientific and technical research personnel in recent years more and more comes into one's own.But, because the supply of the reason ， She Minnow such as catching intensity is excessive, environmental pollution cannot the amount of meeting the need of market.Therefore ， She Minnow propagate artificially imperative, but existing document and patent are all without the method relating to She Minnow artificial induced spawning, and this problem cannot solve the development limiting She Minnow aquaculture.The mating season of ， She Minnow relatively early compared with other fish, to breed water temperature on the low side, and this causes the Effect time of its artificial induced spawning relatively long.We are according to the particularity of She Minnow, and through test repeatedly with grope, provide the induced spawning method of a set of She Minnow, the development propagating industry Wei She Minnow artificially lays the foundation.
Summary of the invention
The object of the invention is to be to overcome prior art deficiency, it is a kind of method that there are provided She Minnow artificial induced spawning, easy to implement the method, easy and simple to handle, spawning effect is good, effectively solve the problem of snake Minnow artificial induced spawning, make snake Minnow spawning rate reach about 85%, fertilization rate reaches more than 80%.
In order to realize above-mentioned object, the present invention adopts following technical measures:
A method for She Minnow artificial induced spawning, the steps include:
A, ripe She Minnow parent is fished for water outlet after, put into indoor spawning pond and support temporarily and adapt to 2-4h.
B, hasten parturition when starting, She Minnow parent pulled out, after She Minnow is fixing, inject oxytocic drug with have gentle hands is light, adopt the injection of pectoral fin base portion, injection angles and fish body are about 45° angle, enter pin about 1.0cm, and a pin is injected;
C, every female parent of tail inject 0.2ml and to hasten parturition liquid, and male parent injects 0.1ml and to hasten parturition liquid, and is later than female parent 2h inject time, and the parent injected puts into spawning pond immediately, avoids long-time operation to cause stress reaction.
D, reach Effect time after, after pulling raun out spawning pond, dry body surface with dry towel, the ovum of maturation clamp-oned in dry plastic basin;
The sperm of E, acquisition fish, removes the spermary of milter, is put on filter paper and removes moisture, put into mortar afterwards and grind.
F, the artificial dry fertilization of employing, be fertilized with the physiological saline of 0.5% (mass volume ratio).Female-male proportion is 10:1.
Described oxytocic drug selects carp brain hypophysis (PG), luliberin analog A
2(LHRH-A
2) and Malaysia DOM (DOM) three kinds of drug combinations, dosage of hastening parturition is that every kilogram of parent uses carp brain hypophysis (PG) 3-5mg, luliberin analog A
2(LHRH-A
2) 5-8 μ g and Malaysia DOM (DOM) 2-3mg, milter dosage reduces by half, and the selection of concrete dosage is 12-26 DEG C according to the ripe situation of parent and water temperature (She Minnow suitable water temperature of hastening parturition) suitably adjust.
Parent's male and female after injection separately, are placed in two spawning ponds, the micro-stimulation by running water of discontinuity, promote gonad maturity.
Described injection oxytocic drug uses common injector for medical purpose to complete, and again puts again, make syringe needle expose 1.0cm after syringe needle headgear being cut off part before injection, convenient injection.
Adopt this method She Minnow spawning rate can reach about 85%, fertilization rate about 80%.
The different water temperature of table 1 is Yu the relation of She Minnow Effect time
Water temperature (
between seasonable (h)
Compared with prior art, the invention has the beneficial effects as follows:
She Minnow induced spawning method provided by the invention, convenient operation, avoid the stress damage that excessive operation Dui She Minnow parent causes, the method spawning effect is good, and can improve spawning rate and the fertilization rate of She Minnow parent, the development propagating industry Wei She Minnow artificially is laid a good foundation.Make snake Minnow spawning rate reach more than 85%, fertilization rate reaches about 80%.
Embodiment
Embodiment 1:
The technological means of the present invention's realization is specifically set forth below in conjunction with example.
A method for She Minnow artificial induced spawning, the steps include:
A, morning April 8 draw in the net to obtain snake Minnow parent, select 49 tails maternal, 10 tail male parents.Raun body weight 40-50g, relative fish brood amount about 200ind/g.Parent supported temporarily in spawning pond, carry out artificial induced spawning in the evening.
B, evening April 8 21:00 point, start artificial induced spawning.She Minnow parent pulled out, after She Minnow is fixing, inject oxytocic drug with have gentle hands is light, adopt the injection of pectoral fin base portion, injection angles and fish body are about 45° angle, enter pin about 1.0cm, and a pin injection, the female parent of every tail injects 0.2ml liquid.Described oxytocic drug selects carp brain hypophysis (PG), luliberin analog A
2(LHRH-A
2) and Malaysia DOM (DOM) three kinds of drug combinations, dosage of hastening parturition is that every kilogram of parent uses PG5mg, LHRH-A
27 μ g, DOM3mg, the parent injected puts into spawning pond immediately, avoids long-time operation to cause stress reaction.Spawning pond water temperature 21
C, evening April 8 23:00, start to inject male parent, method of operating (step B) is consistent with raun, and every tail male parent injects 0.1ml liquid, and liquid of hastening parturition is identical with raun (step B).
D, afternoon April 9 13:00, female snake Minnow parent starts to lay eggs, and 13:30 is laid eggs end, Effect time 16.5h.
E, reach Effect time after, after pulling raun out spawning pond, dry body surface with dry towel, the ovum of maturation clamp-oned in dry plastic basin.
The sperm of F, acquisition fish, removes the spermary of milter, is put on filter paper and removes moisture, put into mortar afterwards and grind.Adopt artificial dry fertilization, with the physiological saline fertilization of 0.5%.
Rear statistics of being fertilized has 43 tail raun natural labors, spawning rate 87.8%, obtains ovum amount 31000 altogether.
Evening April 9,23:00 point checked fertilization rate, and stochastical sampling 500 ovum, test under microscope fertilized egg number is 396, and fertilization rate is 79.2%.
Embodiment 2:
Next year we expand the scale of production, and will be advanced to the middle ten days and the last ten days in March mating season, general effect is good.
A method for She Minnow artificial induced spawning, the steps include:
A drew in the net to obtain snake Minnow parent in morning March 20, have chosen 536 tails altogether maternal, 96 tail male parents, and the same day, 21:00 point, started artificial induced spawning.Raun body weight 40-50g, relative fish brood amount about 220ind/g.
B is due to spawning pond water temperature only 19 DEG C, and ocyodinic dosage suitably improves by we, and every kilogram of parent uses PG5mg, LHRH-A
28 μ g, DOM4mg; That night, 21:00 started artificial induced spawning, and hasten parturition liquid configuration, injection volume, injecting method etc. are all identical with embodiment 1.
The C3 month 21 about 16:00 ， She Minnow female parent starts to lay eggs, and about 17:00 completes, Effect time 19h.Lay eggs method, Insemination procedures is all identical with embodiment 1.
The maternal natural labor of 472 tails is added up in random sampling, spawning rate 88.1%, 330,000, common property ovum, secondary daily inspection fertilization rate 83.0%.
The sexual maturity peak of D natural conditions Xia She Minnow is about about mid-March, and parent's fish brood amount, the fish brood amount in this season are all higher, and therefore compared with embodiment 1, spawning rate and the fertilization rate of example 2 are higher, but due to water temperature lower, Effect time is corresponding prolongation also.
Claims (1)
1. a method for snake Minnow artificial induced spawning, the steps include:
After ripe She Minnow parent is fished for water outlet, put into indoor spawning pond and support adaptation 2-4h temporarily;
Hasten parturition when starting, She Minnow parent pulled out, inject oxytocic drug with hand after She Minnow is fixing, adopt the injection of pectoral fin base portion, injection angles and fish body are 45° angle, enter pin 1.0cm, and a pin is injected;
The female parent of every tail injects 0.2ml and to hasten parturition liquid, and male parent injects 0.1ml and to hasten parturition liquid, and be later than female parent 2h inject time, the parent injected puts into spawning pond;
After reaching Effect time, after pulling raun out spawning pond, dry body surface with dry towel, ovum is clamp-oned in dry plastic basin;
Obtain the sperm of fish, the spermary of milter is removed, is put on filter paper and removes moisture, put into mortar afterwards and grind;
Adopt artificial dry fertilization, with the fertilization of the physiological saline of 0.5% mass volume ratio, female-male proportion is 10:1;
Described oxytocic drug selects carp brain hypophysis, luliberin analog A
2with Malaysia DOM three kinds of drug combinations, dosage of hastening parturition is that every kilogram of parent uses carp brain hypophysis 3-5mg, luliberin analog A
25-8 μ g and Malaysia DOM 2-3mg, milter dosage reduces by half;
Parent's male and female after described injection separately, are placed in two spawning ponds, discontinuity stimulation by running water, promote gonad maturity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510703792.3A CN105432513B (en) | 2015-10-26 | 2015-10-26 | A kind of method of She Minnow artificial induced spawnings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510703792.3A CN105432513B (en) | 2015-10-26 | 2015-10-26 | A kind of method of She Minnow artificial induced spawnings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105432513A true CN105432513A (en) | 2016-03-30 |
| CN105432513B CN105432513B (en) | 2018-06-29 |
Family
ID=55543510
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510703792.3A Expired - Fee Related CN105432513B (en) | 2015-10-26 | 2015-10-26 | A kind of method of She Minnow artificial induced spawnings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105432513B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548713A (en) * | 2018-11-20 | 2019-04-02 | 安徽省农业科学院水产研究所 | A kind of artificial fecundation method of stick flower fish |
| CN110178761A (en) * | 2019-06-17 | 2019-08-30 | 江西省水产科学研究所 | A kind of induced spawning method of Wen Minnow |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999331A (en) * | 2010-11-26 | 2011-04-06 | 中国水产科学研究院长江水产研究所 | Breeding method of snakehead by artificial insemination |
| KR101059515B1 (en) * | 2011-05-25 | 2011-08-26 | 전라남도 | Spawning induction by water temperature stimulus of larimichthys polyactis |
| CN102301972A (en) * | 2011-08-05 | 2012-01-04 | 句容市句容水库管理所 | Method for artificially breeding Plagiognathops microlepis |
| KR20120003961U (en) * | 2010-11-27 | 2012-06-07 | 신경철 | Assembly fish spawning box |
-
2015
- 2015-10-26 CN CN201510703792.3A patent/CN105432513B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999331A (en) * | 2010-11-26 | 2011-04-06 | 中国水产科学研究院长江水产研究所 | Breeding method of snakehead by artificial insemination |
| KR20120003961U (en) * | 2010-11-27 | 2012-06-07 | 신경철 | Assembly fish spawning box |
| KR101059515B1 (en) * | 2011-05-25 | 2011-08-26 | 전라남도 | Spawning induction by water temperature stimulus of larimichthys polyactis |
| CN102301972A (en) * | 2011-08-05 | 2012-01-04 | 句容市句容水库管理所 | Method for artificially breeding Plagiognathops microlepis |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109548713A (en) * | 2018-11-20 | 2019-04-02 | 安徽省农业科学院水产研究所 | A kind of artificial fecundation method of stick flower fish |
| CN110178761A (en) * | 2019-06-17 | 2019-08-30 | 江西省水产科学研究所 | A kind of induced spawning method of Wen Minnow |
| CN110178761B (en) * | 2019-06-17 | 2021-10-29 | 江西省水产科学研究所 | Artificial spawning induction method for rhinogobio ventralis |
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| CN105432513B (en) | 2018-06-29 |
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