CN105432293B - Tinosporae cuttage breeding method - Google Patents
Tinosporae cuttage breeding method Download PDFInfo
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- CN105432293B CN105432293B CN201510869891.9A CN201510869891A CN105432293B CN 105432293 B CN105432293 B CN 105432293B CN 201510869891 A CN201510869891 A CN 201510869891A CN 105432293 B CN105432293 B CN 105432293B
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- 238000009395 breeding Methods 0.000 title claims abstract description 46
- 238000005520 cutting process Methods 0.000 claims abstract description 56
- 239000004576 sand Substances 0.000 claims abstract description 38
- 239000007864 aqueous solution Substances 0.000 claims abstract description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 6
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 6
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 239000011159 matrix material Substances 0.000 claims description 37
- 238000001802 infusion Methods 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 239000010455 vermiculite Substances 0.000 claims description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000011084 recovery Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- MXTLAHSTUOXGQF-UHFFFAOYSA-O Jatrorrhizine Chemical compound COC1=CC=C2C=C3C(C=C(C(=C4)O)OC)=C4CC[N+]3=CC2=C1OC MXTLAHSTUOXGQF-UHFFFAOYSA-O 0.000 description 2
- PTPHDVKWAYIFRX-UHFFFAOYSA-N Palmatine Natural products C1C2=C(OC)C(OC)=CC=C2C=C2N1CCC1=C2C=C(OC)C(OC)=C1 PTPHDVKWAYIFRX-UHFFFAOYSA-N 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- QUCQEUCGKKTEBI-UHFFFAOYSA-N palmatine Chemical compound COC1=CC=C2C=C(C3=C(C=C(C(=C3)OC)OC)CC3)[N+]3=CC2=C1OC QUCQEUCGKKTEBI-UHFFFAOYSA-N 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- YLRXAIKMLINXQY-ZDUSSCGKSA-O (S)-magnoflorine Chemical compound C1=C(OC)C(O)=C2C3=C(O)C(OC)=CC=C3C[C@@H]3[N+](C)(C)CCC1=C23 YLRXAIKMLINXQY-ZDUSSCGKSA-O 0.000 description 1
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- AQASRZOCERRGBL-UHFFFAOYSA-N Dauricine Natural products CN1CCC2=CC(OC)=C(OC)C=C2C1CC1=CC=C(O)C(OC2=CC=C(C=C2)CC2N(C)CCC=3C=C(C(=CC=32)OC)OC)=C1 AQASRZOCERRGBL-UHFFFAOYSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010017553 Furuncle Diseases 0.000 description 1
- KYEAXNAYHSCLMT-CVVGWEDFSA-N Magnoflorine Natural products C[C@H]1OC=C2[C@@H]3[C@@H]1CN4CCc5c([nH]c6ccccc56)[C@@H]4[C@@H]3OC2=O KYEAXNAYHSCLMT-CVVGWEDFSA-N 0.000 description 1
- 240000007817 Olea europaea Species 0.000 description 1
- 206010068319 Oropharyngeal pain Diseases 0.000 description 1
- 201000007100 Pharyngitis Diseases 0.000 description 1
- 241000133430 Tinospora Species 0.000 description 1
- 241001627788 Tinospora capillipes Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 206010000269 abscess Diseases 0.000 description 1
- 230000000146 antalgic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- AALLCALQGXXWNA-DURQJQQASA-N columbin Chemical compound C=1([C@@H]2C[C@@]3([C@H](C(=O)O2)CC[C@@]2(C)[C@]4(O)C(=O)O[C@@H](C=C4)[C@@H]32)C)C=COC=1 AALLCALQGXXWNA-DURQJQQASA-N 0.000 description 1
- OKIIUWUOOIYUGL-UHFFFAOYSA-N columbin Natural products OC12C=CC(OC1=O)C3C4CC(OC(=O)C4CCC23)c5cocc5 OKIIUWUOOIYUGL-UHFFFAOYSA-N 0.000 description 1
- AQASRZOCERRGBL-ROJLCIKYSA-N dauricine Chemical compound CN1CCC2=CC(OC)=C(OC)C=C2[C@H]1CC1=CC=C(O)C(OC2=CC=C(C=C2)C[C@H]2N(C)CCC=3C=C(C(=CC=32)OC)OC)=C1 AQASRZOCERRGBL-ROJLCIKYSA-N 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000010159 dioecy Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- AALLCALQGXXWNA-UHFFFAOYSA-N isocolumbin Natural products O1C(=O)C(CCC2(C)C3(O)C(=O)OC(C=C3)C32)C3(C)CC1C=1C=COC=1 AALLCALQGXXWNA-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- UEAPAHNNFSZHMW-UHFFFAOYSA-N stepahnine Natural products COC1=CC=CC(C2=C34)=C1CC3N(C)CCC4=CC1=C2OCO1 UEAPAHNNFSZHMW-UHFFFAOYSA-N 0.000 description 1
- UEAPAHNNFSZHMW-CQSZACIVSA-N stephanine Chemical compound CN([C@@H]1CC2=C(C3=C11)C=CC=C2OC)CCC1=CC1=C3OCO1 UEAPAHNNFSZHMW-CQSZACIVSA-N 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Hydroponics (AREA)
Abstract
The invention discloses a kind of tinosporae cuttage breeding methods, including:1) cuttings is prepared, the tinosporae tendril of semi-lignified is chosen, takes the stem section of one leaf of one section band as cuttings, it is spare;2) aqueous solution of taking root is prepared, chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, the volume for adding water to mixed liquor thereto is 20 times of the alcohol by volume, and must take root aqueous solution, spare;3) cuttings processing and cuttage, the part that the length by the cuttings of step 1) preparation close to lower end is 1.5~2.5cm are soaked in the 30~60min of aqueous solution of taking root, pull out, dried in shady place, processed cuttings cuttage is cultivated in sand bed;Tinosporae cuttage breeding method of the present invention is applied to the cutting propagation nursery of tinosporae, and cuttage survival rate reaches 90% or more, the protection and recovery in imminent danger of wild resource in seedling problem in short supply and nature can be effectively relieved.
Description
Technical field
The present invention relates to a kind of tinosporae propagation methods.It is more particularly related to a kind of tinosporae cutting propagation
Method.
Background technology
Tinosporae (Tinospora capillipes Gagnep.) is the evergreen winding liana of Menispermaceae Tinospora,
Main product has effects that clearing heat and detoxicating, relieving sore throat and acesodyne in Sichuan, Hunan, Guangxi, Hubei, Guizhou and other places, cure mainly abscess of throat,
Ulcer serious case of furuncle, diarrhea, dysentery, gastral cavity abdomen heat pain etc..Tinosporae root tuber contains palmatine (Palnmtine) and columbin
(Colmtfifin), the ingredients such as palmatine, jateorrhizine, jateorrhizine, stephanine, Dauricine and magnoflorine, it is civil
It is widely used in treatment stomachache.Modern pharmacology research shows that tinosporae has apparent anti-inflammatory and antalgic, antibacterial, antitumor, drop blood
The effects that sugar, resisting stress and removing toxic substances, and it is called the natural drug of alternative antibiotic.Due to excavating for a long time, tinosporae is wild
Resource is increasingly exhausted, artificial growth and foster it is imperative.In addition, tinosporae dioecism, pollination is influenced greatly by environment,
Natural seed setting rate is low.It being found in resource investigation and in introducing a fine variety, staminiferous plant is far more than female plant (about 50: 1), and as a result chance greatly reduces,
And seed has apparent rest period, germination percentage relatively low.Therefore, introduces a collection shortage is the main reason for tinosporae resource falls sharply.In addition
Recently as being continuously increased for market demand, the collection capacity of wild resource is gradually increased, wild tinosporae resource is on the verge of
Exhaustion, it is cultivated at present introduce a fine variety breeding etc. technologies have not been reported.Therefore cuttage breeding method is utilized, can fast and effectively be increased
The generation for the problems such as adding its breeding coefficient, avoiding decline, variation is conducive to the merit for keeping seedling, and improving seedling makes
Safety provides technical support to resources of medicinal plant regeneration in short supply in imminent danger and sustainable utilization.
Invention content
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of tinosporae cuttage breeding method, tinosporae cutting propagation of the invention
Method is applied to the cutting propagation nursery of tinosporae, and cuttage survival rate reaches 90% or more, seedling problem in short supply can be effectively relieved
And in nature wild resource protection and recovery in imminent danger.
In order to realize these purposes and other advantages according to the present invention, a kind of tinosporae cuttage breeding method is provided,
Including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
250~500mg in proportion:50~100mg:It is mixed that 50ml chooses methyl α-naphthyl acetate, indolebutyric acid and 95% alcohol respectively
It closes, the volume for adding water to mixed liquor thereto is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by cuttings prepared by step 1) close to lower end is 1.5~2.5cm be soaked in it is described take root it is water-soluble
30~60min of liquid, is pulled out, is dried in shady place, and processed cuttings cuttage is cultivated in sand bed.
Preferably, the tinosporae cuttage breeding method further includes 4 after the step 3)) it manages after cuttage:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
Preferably, the tinosporae cuttage breeding method, cutting time are the 17 of annual late May to early July
~19 points.
Preferably, the tinosporae cuttage breeding method, the sand bed is interior to be equipped with matrix, and the matrix is volume ratio
It is 1:1:2 dry moss, sand and vermiculite.
Preferably, the tinosporae cuttage breeding method, the sand bed include that opening is set in a rectangular parallelepiped shape and upward
The bed body set, and be fixed on multiple tube bodies with hollow structure of the bed body bottom vertically, tube wall be include internal layer
It is a cavity being connected to, the internal layer and outer layer table of the tube wall between the internal layer and the outer layer with the double-layer structure of outer layer
Face opens up multiple through-holes, and each through-hole surfaces covers one layer of ventilated membrane, each the cavity of the tube wall with the external world
Air blower connection, to be embodied as infusion of air in the cavity.
Preferably, the tinosporae cuttage breeding method, in the step 3), by processed cuttings cuttage in sand
It is cultivated in bed, specially:
Matrix is put into the hollow structure of tube body so that matrix height is the 1/3 of the tube body height, is spread in stromal surface
If multilayer is inserted into hollow structure in circular wire netting, then by processed cuttings by the hole among the wire netting
Medium Culture finally continues to put into matrix into the hollow structure of the tube body so that matrix height reaches the tube body height
2/3, when equal one cuttings of cuttage in each tube body, the part to the bed body except the tube body puts into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body, duration 20min.
Preferably, the tinosporae cuttage breeding method, the sand bed are full exposure spraying sand bed.
Preferably, the tinosporae cuttage breeding method in the step 3), dries, specially in shady place:In
Shady place is dried in the air does not drip to surface.
The present invention includes at least following advantageous effect:
(1) tinosporae cuttage breeding method of the invention is applied to the cutting propagation nursery of tinosporae, and cuttage survival rate reaches
To 90% or more, the protection and recovery in imminent danger of wild resource in seedling problem in short supply and nature can be effectively relieved;
(2) present invention is multiple with hollow structure by the way that tinosporae cuttage to be arranged in specific sand bed, in sand bed
Tube body, using air is passed through in the hollow structure that air blower is tube body, to provide more oxygen for the growth of tinosporae cuttings
Gas provides its survival rate and growth rate;
(3) during cuttage, it is laid with multilayer wire netting among matrix, reinforces the gas permeability of Medium Culture, golden fruit is provided
The survival rate and growth rate of olive cuttage.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Description of the drawings
Fig. 1 is the structural schematic diagram of sand bed of the present invention.
Specific implementation mode
The present invention is described in further detail with reference to the accompanying drawings and examples, to enable those skilled in the art's reference
Specification word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
<Embodiment 1>
The present invention provides a kind of tinosporae cuttage breeding method, including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
250mg in proportion:50mg:50ml chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, adds thereto
The volume of water to mixed liquor is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by the cuttings of step 1) preparation close to lower end is 1.5cm is soaked in the aqueous solution of taking root
30min is pulled out, is dried in shady place, and processed cuttings cuttage is cultivated in sand bed.
The tinosporae cuttage breeding method further includes 4 after the step 3)) it manages after cuttage:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
The tinosporae cuttage breeding method, cutting time are 17 points of annual late May to early July.
The tinosporae cuttage breeding method, the sand bed is interior to be equipped with matrix, and the matrix is that volume ratio is 1:1:2
Dry moss, sand and vermiculite.
As shown in Figure 1, the tinosporae cuttage breeding method, the sand bed includes that opening is set in a rectangular parallelepiped shape and upward
The bed body 1 set, and be fixed on multiple tube bodies 2 with hollow structure of the bed body bottom vertically, tube wall be include interior
The double-layer structure of layer and outer layer is a cavity being connected to, the internal layer and outer layer of the tube wall between the internal layer and the outer layer
Surface opens up multiple through-holes, and each through-hole surfaces covers one layer of ventilated membrane, each the cavity of the tube wall with outside
The air blower 3 on boundary is connected to, to be embodied as infusion of air in the cavity of the tube wall.
In the step 3), processed cuttings cuttage is cultivated in sand bed for the tinosporae cuttage breeding method,
Specially:
Matrix is put into the hollow structure of tube body 2 so that matrix height is the 1/3 of the tube body height, in stromal surface
Multilayer is laid in circular wire netting, then processed cuttings is inserted into hollow structure by the hole among the wire netting
Medium Culture, finally continue to put into matrix into the hollow structure of the tube body 2 so that matrix height reach the tube body height
2/3, when equal one cuttings of cuttage in each tube body 2, to the bed body 1 except the tube body 2 part put into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body 2, duration 20min.
The tinosporae cuttage breeding method, the sand bed are full exposure spraying sand bed.
The tinosporae cuttage breeding method in the step 3), dries, specially in shady place:It dries in the air in shady place
It does not drip to surface.
<Embodiment 2>
The present invention provides a kind of tinosporae cuttage breeding method, including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
350mg in proportion:70mg:50ml chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, adds thereto
The volume of water to mixed liquor is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by the cuttings of step 1) preparation close to lower end is 2cm is soaked in the aqueous solution 50min of taking root,
It pulls out, is dried in shady place, processed cuttings cuttage is cultivated in sand bed.
The tinosporae cuttage breeding method further includes 4 after the step 3)) it manages after cuttage:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
The tinosporae cuttage breeding method, cutting time are 18 points of annual late May to early July.
The tinosporae cuttage breeding method, the sand bed is interior to be equipped with matrix, and the matrix is that volume ratio is 1:1:2
Dry moss, sand and vermiculite.
As shown in Figure 1, the tinosporae cuttage breeding method, the sand bed includes that opening is set in a rectangular parallelepiped shape and upward
The bed body 1 set, and be fixed on multiple tube bodies 2 with hollow structure of the bed body bottom vertically, tube wall be include interior
The double-layer structure of layer and outer layer is a cavity being connected to, the internal layer and outer layer of the tube wall between the internal layer and the outer layer
Surface opens up multiple through-holes, and each through-hole surfaces covers one layer of ventilated membrane, each the cavity of the tube wall with outside
The air blower 3 on boundary is connected to, to be embodied as infusion of air in the cavity of the tube wall.
In the step 3), processed cuttings cuttage is cultivated in sand bed for the tinosporae cuttage breeding method,
Specially:
Matrix is put into the hollow structure of tube body 2 so that matrix height is the 1/3 of the tube body height, in stromal surface
Multilayer is laid in circular wire netting, then processed cuttings is inserted into hollow structure by the hole among the wire netting
Medium Culture, finally continue to put into matrix into the hollow structure of the tube body 2 so that matrix height reach the tube body height
2/3, when equal one cuttings of cuttage in each tube body 2, to the bed body 1 except the tube body 2 part put into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body 2, duration 20min.
The tinosporae cuttage breeding method, the sand bed are full exposure spraying sand bed.
The tinosporae cuttage breeding method in the step 3), dries, specially in shady place:It dries in the air in shady place
It does not drip to surface.
<Embodiment 3>
The present invention provides a kind of tinosporae cuttage breeding method, including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
500mg in proportion:100mg:50ml chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, thereto
The volume for adding water to mixed liquor is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by the cuttings of step 1) preparation close to lower end is 2.5cm is soaked in the aqueous solution of taking root
60min is pulled out, is dried in shady place, and processed cuttings cuttage is cultivated in sand bed.
The tinosporae cuttage breeding method further includes 4 after the step 3)) it manages after cuttage:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
The tinosporae cuttage breeding method, cutting time are 19 points of annual late May to early July.
The tinosporae cuttage breeding method, the sand bed is interior to be equipped with matrix, and the matrix is that volume ratio is 1:1:2
Dry moss, sand and vermiculite.
As shown in Figure 1, the tinosporae cuttage breeding method, the sand bed includes that opening is set in a rectangular parallelepiped shape and upward
The bed body 1 set, and be fixed on multiple tube bodies 2 with hollow structure of the bed body bottom vertically, tube wall be include interior
The double-layer structure of layer and outer layer is a cavity being connected to, the internal layer and outer layer of the tube wall between the internal layer and the outer layer
Surface opens up multiple through-holes, and each through-hole surfaces covers one layer of ventilated membrane, each the cavity of the tube wall with outside
The air blower 3 on boundary is connected to, to be embodied as infusion of air in the cavity of the tube wall.
In the step 3), processed cuttings cuttage is cultivated in sand bed for the tinosporae cuttage breeding method,
Specially:
Matrix is put into the hollow structure of tube body 2 so that matrix height is the 1/3 of the tube body height, in stromal surface
Multilayer is laid in circular wire netting, then processed cuttings is inserted into hollow structure by the hole among the wire netting
Medium Culture, finally continue to put into matrix into the hollow structure of the tube body 2 so that matrix height reach the tube body height
2/3, when equal one cuttings of cuttage in each tube body 2, to the bed body 1 except the tube body 2 part put into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body 2, duration 20min.
The tinosporae cuttage breeding method, the sand bed are full exposure spraying sand bed.
The tinosporae cuttage breeding method in the step 3), dries, specially in shady place:It dries in the air in shady place
It does not drip to surface.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and drawings and examples shown and described herein.
Claims (5)
1. a kind of tinosporae cuttage breeding method, which is characterized in that including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
250~500mg in proportion:50~100mg:50ml chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, to
The volume for wherein adding water to mixed liquor is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by the cuttings of step 1) preparation close to lower end is 1.5~2.5cm is soaked in the aqueous solution 30 of taking root
~60min, is pulled out, is dried in shady place, and processed cuttings cuttage is cultivated in sand bed;
Matrix is housed, the matrix is that volume ratio is 1 in the sand bed:1:2 dry moss, sand and vermiculite;
The sand bed includes the bed body that in a rectangular parallelepiped shape and opening is arranged upward, and is fixed on the more of the bed body bottom vertically
A tube body with hollow structure, tube wall are the double-layer structure for including internal layer and outer layer, between the internal layer and the outer layer
For the cavity of a connection, the internal layer and superficies of the tube wall open up multiple through-holes, and each through-hole surfaces cover
The cavity of one layer of ventilated membrane, each tube wall is connected to extraneous air blower, to be embodied as infusion of air in the cavity;
In the step 3), processed cuttings cuttage is cultivated in sand bed, specially:
Matrix is put into the hollow structure of tube body so that matrix height is the 1/3 of the tube body height, is laid in stromal surface more
Layer passes through the matrix of the hole insertion hollow structure among the wire netting in circular wire netting, then by processed cuttings
It is interior, finally continue to put into matrix into the hollow structure of the tube body so that matrix height reaches the 2/3 of the tube body height,
When equal one cuttings of cuttage in each tube body, the part to the bed body except the tube body puts into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body, duration 20min.
2. tinosporae cuttage breeding method as described in claim 1, which is characterized in that further include 4 after the step 3)) skewer
It is managed after inserting:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
3. tinosporae cuttage breeding method as described in claim 1, which is characterized in that cutting time is annual late May to 7
17~19 points of the first tenday period of a month moon.
4. tinosporae cuttage breeding method as described in claim 1, which is characterized in that the sand bed is that full exposure spraying is husky
Bed.
5. tinosporae cuttage breeding method as described in claim 1, which is characterized in that in the step 3), dry in the air in shady place
It is dry, specially:It dries in the air in shady place and does not drip to surface.
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CN106212172A (en) * | 2016-07-29 | 2016-12-14 | 广东神州木兰园林有限公司 | Poem beautiful jade leads to cuttage and seedling culture method with a smile |
CN106472158A (en) * | 2016-10-14 | 2017-03-08 | 柳州环山科技有限公司 | A kind of Radix Tinosporae cuttage seedling cultivating substrate and preparation method thereof |
CN107637292A (en) * | 2017-11-14 | 2018-01-30 | 广西壮族自治区药用植物园 | That rattan cuttage breeding method of the five fingers |
CN108934867B (en) * | 2018-07-02 | 2021-01-01 | 广西壮族自治区药用植物园 | Intensive and directional planting method for tinospora cordifolia |
CN110432102B (en) * | 2019-07-25 | 2022-09-06 | 华润三九(雅安)药业有限公司 | Tinospora cordifolia cutting propagation method |
CN111418364A (en) * | 2020-05-11 | 2020-07-17 | 广西壮族自治区药用植物园 | Cutting propagation method of vernonia galophylla |
CN111713327B (en) * | 2020-06-30 | 2022-01-25 | 四川省中医药科学院 | Planting method for intercropping tinospora root and pear trees |
CN114793656B (en) * | 2022-06-15 | 2024-03-19 | 华润三九医药股份有限公司 | Cutting propagation method of radix tinosporae seedlings |
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Application publication date: 20160330 Assignee: Tengxian Mingfeng Agricultural Technology Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980046049 Denomination of invention: Cutting Propagation Method of Golden Fruit Olive Granted publication date: 20180821 License type: Common License Record date: 20231108 |