CN105432293B - Tinosporae cuttage breeding method - Google Patents

Tinosporae cuttage breeding method Download PDF

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Publication number
CN105432293B
CN105432293B CN201510869891.9A CN201510869891A CN105432293B CN 105432293 B CN105432293 B CN 105432293B CN 201510869891 A CN201510869891 A CN 201510869891A CN 105432293 B CN105432293 B CN 105432293B
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cuttage
tinosporae
cuttings
matrix
breeding method
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CN105432293A (en
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黄芩芬
韦荣昌
韦范
覃芳
潘丽梅
谷筱玉
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Guangxi Botanical Garden of Medicinal Plants
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Guangxi Botanical Garden of Medicinal Plants
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Hydroponics (AREA)

Abstract

The invention discloses a kind of tinosporae cuttage breeding methods, including:1) cuttings is prepared, the tinosporae tendril of semi-lignified is chosen, takes the stem section of one leaf of one section band as cuttings, it is spare;2) aqueous solution of taking root is prepared, chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, the volume for adding water to mixed liquor thereto is 20 times of the alcohol by volume, and must take root aqueous solution, spare;3) cuttings processing and cuttage, the part that the length by the cuttings of step 1) preparation close to lower end is 1.5~2.5cm are soaked in the 30~60min of aqueous solution of taking root, pull out, dried in shady place, processed cuttings cuttage is cultivated in sand bed;Tinosporae cuttage breeding method of the present invention is applied to the cutting propagation nursery of tinosporae, and cuttage survival rate reaches 90% or more, the protection and recovery in imminent danger of wild resource in seedling problem in short supply and nature can be effectively relieved.

Description

Tinosporae cuttage breeding method
Technical field
The present invention relates to a kind of tinosporae propagation methods.It is more particularly related to a kind of tinosporae cutting propagation Method.
Background technology
Tinosporae (Tinospora capillipes Gagnep.) is the evergreen winding liana of Menispermaceae Tinospora, Main product has effects that clearing heat and detoxicating, relieving sore throat and acesodyne in Sichuan, Hunan, Guangxi, Hubei, Guizhou and other places, cure mainly abscess of throat, Ulcer serious case of furuncle, diarrhea, dysentery, gastral cavity abdomen heat pain etc..Tinosporae root tuber contains palmatine (Palnmtine) and columbin (Colmtfifin), the ingredients such as palmatine, jateorrhizine, jateorrhizine, stephanine, Dauricine and magnoflorine, it is civil It is widely used in treatment stomachache.Modern pharmacology research shows that tinosporae has apparent anti-inflammatory and antalgic, antibacterial, antitumor, drop blood The effects that sugar, resisting stress and removing toxic substances, and it is called the natural drug of alternative antibiotic.Due to excavating for a long time, tinosporae is wild Resource is increasingly exhausted, artificial growth and foster it is imperative.In addition, tinosporae dioecism, pollination is influenced greatly by environment, Natural seed setting rate is low.It being found in resource investigation and in introducing a fine variety, staminiferous plant is far more than female plant (about 50: 1), and as a result chance greatly reduces, And seed has apparent rest period, germination percentage relatively low.Therefore, introduces a collection shortage is the main reason for tinosporae resource falls sharply.In addition Recently as being continuously increased for market demand, the collection capacity of wild resource is gradually increased, wild tinosporae resource is on the verge of Exhaustion, it is cultivated at present introduce a fine variety breeding etc. technologies have not been reported.Therefore cuttage breeding method is utilized, can fast and effectively be increased The generation for the problems such as adding its breeding coefficient, avoiding decline, variation is conducive to the merit for keeping seedling, and improving seedling makes Safety provides technical support to resources of medicinal plant regeneration in short supply in imminent danger and sustainable utilization.
Invention content
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of tinosporae cuttage breeding method, tinosporae cutting propagation of the invention Method is applied to the cutting propagation nursery of tinosporae, and cuttage survival rate reaches 90% or more, seedling problem in short supply can be effectively relieved And in nature wild resource protection and recovery in imminent danger.
In order to realize these purposes and other advantages according to the present invention, a kind of tinosporae cuttage breeding method is provided, Including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
250~500mg in proportion:50~100mg:It is mixed that 50ml chooses methyl α-naphthyl acetate, indolebutyric acid and 95% alcohol respectively It closes, the volume for adding water to mixed liquor thereto is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by cuttings prepared by step 1) close to lower end is 1.5~2.5cm be soaked in it is described take root it is water-soluble 30~60min of liquid, is pulled out, is dried in shady place, and processed cuttings cuttage is cultivated in sand bed.
Preferably, the tinosporae cuttage breeding method further includes 4 after the step 3)) it manages after cuttage:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
Preferably, the tinosporae cuttage breeding method, cutting time are the 17 of annual late May to early July ~19 points.
Preferably, the tinosporae cuttage breeding method, the sand bed is interior to be equipped with matrix, and the matrix is volume ratio It is 1:1:2 dry moss, sand and vermiculite.
Preferably, the tinosporae cuttage breeding method, the sand bed include that opening is set in a rectangular parallelepiped shape and upward The bed body set, and be fixed on multiple tube bodies with hollow structure of the bed body bottom vertically, tube wall be include internal layer It is a cavity being connected to, the internal layer and outer layer table of the tube wall between the internal layer and the outer layer with the double-layer structure of outer layer Face opens up multiple through-holes, and each through-hole surfaces covers one layer of ventilated membrane, each the cavity of the tube wall with the external world Air blower connection, to be embodied as infusion of air in the cavity.
Preferably, the tinosporae cuttage breeding method, in the step 3), by processed cuttings cuttage in sand It is cultivated in bed, specially:
Matrix is put into the hollow structure of tube body so that matrix height is the 1/3 of the tube body height, is spread in stromal surface If multilayer is inserted into hollow structure in circular wire netting, then by processed cuttings by the hole among the wire netting Medium Culture finally continues to put into matrix into the hollow structure of the tube body so that matrix height reaches the tube body height 2/3, when equal one cuttings of cuttage in each tube body, the part to the bed body except the tube body puts into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body, duration 20min.
Preferably, the tinosporae cuttage breeding method, the sand bed are full exposure spraying sand bed.
Preferably, the tinosporae cuttage breeding method in the step 3), dries, specially in shady place:In Shady place is dried in the air does not drip to surface.
The present invention includes at least following advantageous effect:
(1) tinosporae cuttage breeding method of the invention is applied to the cutting propagation nursery of tinosporae, and cuttage survival rate reaches To 90% or more, the protection and recovery in imminent danger of wild resource in seedling problem in short supply and nature can be effectively relieved;
(2) present invention is multiple with hollow structure by the way that tinosporae cuttage to be arranged in specific sand bed, in sand bed Tube body, using air is passed through in the hollow structure that air blower is tube body, to provide more oxygen for the growth of tinosporae cuttings Gas provides its survival rate and growth rate;
(3) during cuttage, it is laid with multilayer wire netting among matrix, reinforces the gas permeability of Medium Culture, golden fruit is provided The survival rate and growth rate of olive cuttage.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this The research and practice of invention and be understood by the person skilled in the art.
Description of the drawings
Fig. 1 is the structural schematic diagram of sand bed of the present invention.
Specific implementation mode
The present invention is described in further detail with reference to the accompanying drawings and examples, to enable those skilled in the art's reference Specification word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more The presence or addition of a other elements or combinations thereof.
<Embodiment 1>
The present invention provides a kind of tinosporae cuttage breeding method, including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
250mg in proportion:50mg:50ml chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, adds thereto The volume of water to mixed liquor is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by the cuttings of step 1) preparation close to lower end is 1.5cm is soaked in the aqueous solution of taking root 30min is pulled out, is dried in shady place, and processed cuttings cuttage is cultivated in sand bed.
The tinosporae cuttage breeding method further includes 4 after the step 3)) it manages after cuttage:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
The tinosporae cuttage breeding method, cutting time are 17 points of annual late May to early July.
The tinosporae cuttage breeding method, the sand bed is interior to be equipped with matrix, and the matrix is that volume ratio is 1:1:2 Dry moss, sand and vermiculite.
As shown in Figure 1, the tinosporae cuttage breeding method, the sand bed includes that opening is set in a rectangular parallelepiped shape and upward The bed body 1 set, and be fixed on multiple tube bodies 2 with hollow structure of the bed body bottom vertically, tube wall be include interior The double-layer structure of layer and outer layer is a cavity being connected to, the internal layer and outer layer of the tube wall between the internal layer and the outer layer Surface opens up multiple through-holes, and each through-hole surfaces covers one layer of ventilated membrane, each the cavity of the tube wall with outside The air blower 3 on boundary is connected to, to be embodied as infusion of air in the cavity of the tube wall.
In the step 3), processed cuttings cuttage is cultivated in sand bed for the tinosporae cuttage breeding method, Specially:
Matrix is put into the hollow structure of tube body 2 so that matrix height is the 1/3 of the tube body height, in stromal surface Multilayer is laid in circular wire netting, then processed cuttings is inserted into hollow structure by the hole among the wire netting Medium Culture, finally continue to put into matrix into the hollow structure of the tube body 2 so that matrix height reach the tube body height 2/3, when equal one cuttings of cuttage in each tube body 2, to the bed body 1 except the tube body 2 part put into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body 2, duration 20min.
The tinosporae cuttage breeding method, the sand bed are full exposure spraying sand bed.
The tinosporae cuttage breeding method in the step 3), dries, specially in shady place:It dries in the air in shady place It does not drip to surface.
<Embodiment 2>
The present invention provides a kind of tinosporae cuttage breeding method, including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
350mg in proportion:70mg:50ml chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, adds thereto The volume of water to mixed liquor is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by the cuttings of step 1) preparation close to lower end is 2cm is soaked in the aqueous solution 50min of taking root, It pulls out, is dried in shady place, processed cuttings cuttage is cultivated in sand bed.
The tinosporae cuttage breeding method further includes 4 after the step 3)) it manages after cuttage:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
The tinosporae cuttage breeding method, cutting time are 18 points of annual late May to early July.
The tinosporae cuttage breeding method, the sand bed is interior to be equipped with matrix, and the matrix is that volume ratio is 1:1:2 Dry moss, sand and vermiculite.
As shown in Figure 1, the tinosporae cuttage breeding method, the sand bed includes that opening is set in a rectangular parallelepiped shape and upward The bed body 1 set, and be fixed on multiple tube bodies 2 with hollow structure of the bed body bottom vertically, tube wall be include interior The double-layer structure of layer and outer layer is a cavity being connected to, the internal layer and outer layer of the tube wall between the internal layer and the outer layer Surface opens up multiple through-holes, and each through-hole surfaces covers one layer of ventilated membrane, each the cavity of the tube wall with outside The air blower 3 on boundary is connected to, to be embodied as infusion of air in the cavity of the tube wall.
In the step 3), processed cuttings cuttage is cultivated in sand bed for the tinosporae cuttage breeding method, Specially:
Matrix is put into the hollow structure of tube body 2 so that matrix height is the 1/3 of the tube body height, in stromal surface Multilayer is laid in circular wire netting, then processed cuttings is inserted into hollow structure by the hole among the wire netting Medium Culture, finally continue to put into matrix into the hollow structure of the tube body 2 so that matrix height reach the tube body height 2/3, when equal one cuttings of cuttage in each tube body 2, to the bed body 1 except the tube body 2 part put into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body 2, duration 20min.
The tinosporae cuttage breeding method, the sand bed are full exposure spraying sand bed.
The tinosporae cuttage breeding method in the step 3), dries, specially in shady place:It dries in the air in shady place It does not drip to surface.
<Embodiment 3>
The present invention provides a kind of tinosporae cuttage breeding method, including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
500mg in proportion:100mg:50ml chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, thereto The volume for adding water to mixed liquor is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by the cuttings of step 1) preparation close to lower end is 2.5cm is soaked in the aqueous solution of taking root 60min is pulled out, is dried in shady place, and processed cuttings cuttage is cultivated in sand bed.
The tinosporae cuttage breeding method further includes 4 after the step 3)) it manages after cuttage:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
The tinosporae cuttage breeding method, cutting time are 19 points of annual late May to early July.
The tinosporae cuttage breeding method, the sand bed is interior to be equipped with matrix, and the matrix is that volume ratio is 1:1:2 Dry moss, sand and vermiculite.
As shown in Figure 1, the tinosporae cuttage breeding method, the sand bed includes that opening is set in a rectangular parallelepiped shape and upward The bed body 1 set, and be fixed on multiple tube bodies 2 with hollow structure of the bed body bottom vertically, tube wall be include interior The double-layer structure of layer and outer layer is a cavity being connected to, the internal layer and outer layer of the tube wall between the internal layer and the outer layer Surface opens up multiple through-holes, and each through-hole surfaces covers one layer of ventilated membrane, each the cavity of the tube wall with outside The air blower 3 on boundary is connected to, to be embodied as infusion of air in the cavity of the tube wall.
In the step 3), processed cuttings cuttage is cultivated in sand bed for the tinosporae cuttage breeding method, Specially:
Matrix is put into the hollow structure of tube body 2 so that matrix height is the 1/3 of the tube body height, in stromal surface Multilayer is laid in circular wire netting, then processed cuttings is inserted into hollow structure by the hole among the wire netting Medium Culture, finally continue to put into matrix into the hollow structure of the tube body 2 so that matrix height reach the tube body height 2/3, when equal one cuttings of cuttage in each tube body 2, to the bed body 1 except the tube body 2 part put into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body 2, duration 20min.
The tinosporae cuttage breeding method, the sand bed are full exposure spraying sand bed.
The tinosporae cuttage breeding method in the step 3), dries, specially in shady place:It dries in the air in shady place It does not drip to surface.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited In specific details and drawings and examples shown and described herein.

Claims (5)

1. a kind of tinosporae cuttage breeding method, which is characterized in that including:
1) cuttings is prepared
The tinosporae tendril for choosing semi-lignified, takes the stem section of one leaf of one section band as cuttings, spare;
2) aqueous solution of taking root is prepared
250~500mg in proportion:50~100mg:50ml chooses methyl α-naphthyl acetate, indolebutyric acid and the mixing of 95% alcohol respectively, to The volume for wherein adding water to mixed liquor is 20 times of the alcohol by volume, and must take root aqueous solution, spare;
3) cuttings processing and cuttage
The part that length by the cuttings of step 1) preparation close to lower end is 1.5~2.5cm is soaked in the aqueous solution 30 of taking root ~60min, is pulled out, is dried in shady place, and processed cuttings cuttage is cultivated in sand bed;
Matrix is housed, the matrix is that volume ratio is 1 in the sand bed:1:2 dry moss, sand and vermiculite;
The sand bed includes the bed body that in a rectangular parallelepiped shape and opening is arranged upward, and is fixed on the more of the bed body bottom vertically A tube body with hollow structure, tube wall are the double-layer structure for including internal layer and outer layer, between the internal layer and the outer layer For the cavity of a connection, the internal layer and superficies of the tube wall open up multiple through-holes, and each through-hole surfaces cover The cavity of one layer of ventilated membrane, each tube wall is connected to extraneous air blower, to be embodied as infusion of air in the cavity;
In the step 3), processed cuttings cuttage is cultivated in sand bed, specially:
Matrix is put into the hollow structure of tube body so that matrix height is the 1/3 of the tube body height, is laid in stromal surface more Layer passes through the matrix of the hole insertion hollow structure among the wire netting in circular wire netting, then by processed cuttings It is interior, finally continue to put into matrix into the hollow structure of the tube body so that matrix height reaches the 2/3 of the tube body height, When equal one cuttings of cuttage in each tube body, the part to the bed body except the tube body puts into matrix;
After cuttage, every 3 days, air blower was the cavity infusion of air 1 time of the tube body, duration 20min.
2. tinosporae cuttage breeding method as described in claim 1, which is characterized in that further include 4 after the step 3)) skewer It is managed after inserting:
The same day and later every 3 days atomized water sprays after cuttage is primary, until seedling of taking root.
3. tinosporae cuttage breeding method as described in claim 1, which is characterized in that cutting time is annual late May to 7 17~19 points of the first tenday period of a month moon.
4. tinosporae cuttage breeding method as described in claim 1, which is characterized in that the sand bed is that full exposure spraying is husky Bed.
5. tinosporae cuttage breeding method as described in claim 1, which is characterized in that in the step 3), dry in the air in shady place It is dry, specially:It dries in the air in shady place and does not drip to surface.
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CN106212172A (en) * 2016-07-29 2016-12-14 广东神州木兰园林有限公司 Poem beautiful jade leads to cuttage and seedling culture method with a smile
CN106472158A (en) * 2016-10-14 2017-03-08 柳州环山科技有限公司 A kind of Radix Tinosporae cuttage seedling cultivating substrate and preparation method thereof
CN107637292A (en) * 2017-11-14 2018-01-30 广西壮族自治区药用植物园 That rattan cuttage breeding method of the five fingers
CN108934867B (en) * 2018-07-02 2021-01-01 广西壮族自治区药用植物园 Intensive and directional planting method for tinospora cordifolia
CN110432102B (en) * 2019-07-25 2022-09-06 华润三九(雅安)药业有限公司 Tinospora cordifolia cutting propagation method
CN111418364A (en) * 2020-05-11 2020-07-17 广西壮族自治区药用植物园 Cutting propagation method of vernonia galophylla
CN111713327B (en) * 2020-06-30 2022-01-25 四川省中医药科学院 Planting method for intercropping tinospora root and pear trees
CN114793656B (en) * 2022-06-15 2024-03-19 华润三九医药股份有限公司 Cutting propagation method of radix tinosporae seedlings

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CN104705086A (en) * 2013-12-14 2015-06-17 朱振明 Double layer flowerpot
CN203896871U (en) * 2014-05-21 2014-10-29 东莞市信远无纺布有限公司 Agricultural biological oil-tea camellia seedling culturing nutrition bag
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