CN105420074B - A kind of multifunctional bio material disconnecting gear - Google Patents
A kind of multifunctional bio material disconnecting gear Download PDFInfo
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- CN105420074B CN105420074B CN201610034026.7A CN201610034026A CN105420074B CN 105420074 B CN105420074 B CN 105420074B CN 201610034026 A CN201610034026 A CN 201610034026A CN 105420074 B CN105420074 B CN 105420074B
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- magnetosheath
- push rod
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- main shaft
- connection ring
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The present invention provides a kind of multifunctional bio material disconnecting gears, belong to biotechnology.The multifunctional bio material disconnecting gear includes housing, the main shaft push rod through housing, returning device, magnetosheath connection ring and electric device；The both ends of main shaft push rod protrude from housing, and one end is provided with magnetic pole；Magnetosheath connection ring is sheathed on main shaft push rod, and one end that magnetosheath connection ring is in outside housing is provided with magnetosheath；Returning device is set inside housings；And for main shaft push rod to be made to be moved to the direction far from magnetosheath；Electric device is connected with magnetosheath connection ring, and for magnetosheath connection ring to be made to rotate movement (including the magnetosheath being disposed thereon)；This multifunctional bio material disconnecting gear provided by the invention, it is particularly suitable for application to the process of paramagnetic particle method extraction nucleic acid,, with that can cause magnetic bead and its effect there are system (being mostly lysate or eluent) quick separating, the apparatus structure is compact, easy to operate for it.
Description
Technical field
The present invention relates to biotechnology, in particular to a kind of multifunctional bio material disconnecting gear.
Background technology
In biotechnology, the operation mainly taken at present for the separation of substance has centrifugation, filtering, extraction, electrophoresis
Deng.The principle of these methods is to detach different substances by the weight of solid matter, the size of diameter.Such as centrifuge isolate
Matter is different by the quality of solid matter or liquid (proportion) in liquid, generates different interfaces under the influence of centrifugal force
And achieve the purpose that substance and detach；Filtering (including sieving) is reached by the Kong Jing of filter paper (filter membrane) and by the diameter of filter material
The purpose detached to substance.In addition, also having drying, volatilization, extraction, electrophoresis etc. is come by physics or the principles of chemistry mostly
Reach substance separation purpose.
For above-mentioned several traditional separation methods, specifically, application of the centrifugal process in terms of biological substance separation is very
Extensively, history is also very long；Have a disadvantage in that centrifuge tube used must mark clear before centrifuging every time, will be unable to really after otherwise centrifuging
Random sample product label.Mark centrifuge tube time consuming, when especially sample size is more.In addition, centrifugal process can only will be substantially the same
The substance of quality is divided into a certain level (interface), can not isolate equal quality but the substance of different bioactivity.
Filtering, extraction, electrophoresis etc. exist and can only the substance of equal quality be separated to a certain level, can not isolate
The substance of equal quality but different bioactivity, simultaneously for used equipment, there is also operating process is excessively cumbersome, easy production
Raw sample cell mislabels, operating process is difficult to sterile working, and the precision of substance separation is all difficult to control, also can not be according to life
Object activity is come the defects of distinguishing substance.
In recent years, cell, virus, protein, nucleic acid separation process in, the application of immunology principle and nanometer magnetic bead
It is very extensive, such as magnet stand method and automatic extracting instrument used in extraction nucleic acid.For magnet stand method, principle be allow first by
The substance of separation forms a complex with nanometer magnetic bead in the interior reaction bonded of a certain container (such as test tube), and one is then added outside test tube
After magnetic bead is adsorbed onto tube wall by magnetic field, then with certain methods waste liquid is removed.One of shortcoming is that operator must be to each test tube
Then label carries out next step operation；The time that being generally separated a sample needs is 30 minutes, and N number of sample must be N*30 points
Clock.Shortcoming second is that magnetic bead precipitate in a liquid quickly, to keep liquid in all substances be in uniform state, each grasp
The process of work must be added with an extra action-rotation whirlpool instrument mixed processing, this adds increased operating procedure, and dropped significantly
Low service speed.The three of shortcoming are every time with before whirlpool instrument, it is necessary to cover tightly test tube cap, otherwise can not complete operating procedure.It can
See, the above shortcoming cannot all grow with each passing hour with the technology in modern biology field.
For automatic extracting instrument, existing DNA automations extraction equipment majority is using immunology and nanometer in the market
Magnetic bead with reference to principle complete its substance separation process.Its design principle is substantially similar with magnet stand method, only increases certainly
Dynamicization manipulates and other heating functions.One of its shortcoming is that the preparation stage is especially complex；Shortcoming second is that data input very
Trouble, must check verification repeatedly before startup program；As long as the three of shortcoming are done wrong there are one step, sample just gives up by the gross
.Even if the four of shortcoming be after starting, the automation mechanized operation time is also very long (usually to want 30-150 minutes, the reason is that certain steps
Need to be dehydrated), and in existing equipment all using heating drying, etc. the means such as to be dried, when this just substantially prolongs whole operation
Between.
Though it can be seen that above-mentioned magnet stand method and the method for DNA automation extractions compensate for conventional method it is many not
Enough (such as can carry out separate substance according to bioactivity), but the structure design due to existing equipment and technological process have deficiency
Place in operating procedure and is difficult the advantage for embodying modern biology new technique and modern project on the time.
In view of this, it is special to propose the present invention.
Invention content
The first object of the present invention is to provide a kind of multifunctional bio material disconnecting gear, of the prior art to solve
It was operated present in biological substance separation process (separation including carrying out cell, virus, protein, nucleic acid using paramagnetic particle method)
The technical issues of journey is cumbersome, time and effort consuming and separative efficiency are poor.
This multifunctional bio material disconnecting gear provided by the invention is particularly suitable for application to paramagnetic particle method and provides nucleic acid
Process, magnetic bead and its effect there are system (being mostly lysate or eluent) quick separating, the dress can be caused by having
Put it is compact-sized, it is easy to operate.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
The present invention provides a kind of multifunctional bio material disconnecting gear, pushed away including housing, through the main shaft of the housing
Bar, returning device, magnetosheath connection ring and electric device；
The both ends of the main shaft push rod protrude from the housing, and one end is provided with magnetic pole；The magnetosheath connection ring
Be sheathed on the main shaft push rod, and one end for being in outside the housing of the magnetosheath connection ring be provided with magnetosheath (it may be noted that
, in this application, main shaft push rod is not relatively-stationary with magnetosheath connection ring, and main shaft push rod is across magnetosheath connection ring
Central hollow section, so as to forming the structure that a kind of similar magnetosheath connection ring is sleeved on main shaft push rod).
The returning device is arranged on the enclosure interior；And for making the main shaft push rod to the side far from the magnetosheath
To movement；The electric device is connected with the magnetosheath connection ring, and for the magnetosheath connection ring to be made to rotate.
For the multifunctional bio material disconnecting gear, agent structure is housing, is provided on the housing and passes through housing
The main shaft push rod worn；The both ends of main shaft push rod protrude from housing, and have one end to be provided with magnetic pole.In addition, on main shaft push rod
Magnetosheath connection ring is arranged with, and there is provided magnetosheaths for one end (one end outside housing)；In this way under the drive of electric device,
Magnetosheath connection ring and magnetosheath can rotate；And when applying active force to one end (being not provided with magnetic pole) of main shaft push rod, it is main
Axis push rod and its magnetic pole then can realize relative motion with housing, and under the action of returning device, return then occurs for main shaft push rod.
Namely main shaft push rod, when moving back and forth in the housing, magnetic pole is also to be moved back and forth in magnetosheath, and magnetosheath and magnetosheath connection
Ring then rotates under the action of electric device.
During the biological substance separator is applied, such as use it for separation magnetic bead lysate and (contain
The magnetic bead and lysate of nucleic acid are adsorbed)；Magnetosheath is extend into mixed system, pushes main shaft push rod at this time so that magnetic pole
End extend into the bottom of magnetosheath；Under the action of magnetic pole, the magnetic bead in mixed system has been adsorbed on magnetosheath, and by
It removes, so as to fulfill magnetic bead and the quick separating of mixed system.Further, due to magnetosheath be under the drive of electric device can
With rotation, in rotary course, magnetic bead is maintained at by the effect in magnetic field on magnetosheath, and liquid is got rid of under the influence of centrifugal force
It is de- so that magnetic bead and liquid further detach.After the completion of centrifugation, remove the power for being applied to main shaft push rod, main shaft push rod exists
Return under the action of returning device drives magnetic pole to be left from the bottom of magnetosheath；Suction of the magnetic bead adsorbed due to losing magnetic force
Attached effect can carry out the operation of next step so as to be detached from from magnetosheath.
Above-mentioned biological substance separator its during operation, can realize the separation of magnetic bead and mixed system,
And then it can overcome and liquid phase be sucked out from mixed system in the prior art and existing separation is not thorough and magnetic bead is easy to heavy
The defects of dropping and being detached.In addition, the device can further realize the operation of centrifugation, so as to improve by after Beads enrichment
The effect of entire biological substance separation, the methods of can overcoming centrifugation, filtering, extraction biological substance separation, such as can not achieve at the matter
The defects of measuring the separation of heterogeneity substance.
For the device, in order to realize the effect of main shaft push rod returning quickly, in the biological substance point that invention provides
From in device, it is preferred that be further fixedly arranged on wedging position in the housing；The returning device includes the first lantern ring, second
Lantern ring and the first spring；
First lantern ring, the first spring and the second lantern ring are sheathed on respectively on the main shaft push rod, the first set
Ring offsets with the wedging position, and second lantern ring is fixedly connected with the main shaft push rod, the both ends point of first spring
It does not offset with first lantern ring and second lantern ring；
One end in the hull outside is provided with push rod package set on the main shaft push rod；The push rod wraps up sleeve
The one end closed is wrapped in the end of the main shaft push rod, and open one end offsets with second lantern ring.
Wedging position is fixedly disposed in housing, when pushing push rod package set in this way, main shaft push rod and second set
Multiple structures such as ring move (magnetic pole stretches to the bottom of magnetosheath) each along housing；Since the first lantern ring is contacted against in clamping portion
On position, and wedging position is fixed, and when direction movement of second lantern ring towards the first lantern ring, the first spring is compressed.This
When such as discharge additional pressure, since the second lantern ring with main shaft push rod is fixed, so the first spring is applied to the second lantern ring
After active force, further main shaft push rod is driven, so as to fulfill the return of entire main shaft push rod.This kind of return mode uses two
A lantern ring for being placed on main shaft push rod realizes that lantern ring has main shaft push rod the effect of positioning, therefore return effect is good, is not easy to make master
Beat occurs for axis push rod.
Preferably, the enclosure interior is further fixedly arranged on positioning sleeve, and the positioning sleeve is sheathed on the push rod package set
Part in the housing；The inner wall of the positioning sleeve and the outer wall of push rod package set fit.Positioning sleeve will push away
Bar package set and housing are positioned, smooth further so that main shaft push rod is steady in the process of reciprocating.
Preferably, second spring and the magnetosheath disengaging push rod set on the housing is further included；The second spring
One end is fixed on the housing, and one end that the other end is detached from push rod with the magnetosheath offsets, and the magnetosheath is detached from the another of push rod
One end extends to the opening of the housing passed through by magnetic pole.
Magnetosheath is detached from push rod under the effect of external force, is moved along housing, specially towards the housing for being provided with magnetosheath
End motion, during movement, bottom can be abutted on magnetosheath, and then can be detached from magnetosheath, after magnetosheath is detached from, the
Under the action of two springs, magnetosheath is detached from the return original position of push rod.
Optionally, it is additionally provided with circuit board in the housing；The circuit board is electrically connected with the electric device, and described
The motor adjusting knob and charge port for protruding from the housing are additionally provided on circuit board；And it is additionally provided on the housing
For the button switch of control circuit board power supply.
Circuit board integrates various circuits, by motor adjusting knob, can realize that the output power of electric device is adjusted, and
Magnetosheath connection ring and the slewing rate of magnetosheath are directly controlled；Realize the rotational speed regulation of magnetosheath；And by charge port (for convenience
For the sake of, can be set to USB interface), power supply (interior to be set on housing) can be given to realize charging effect；In addition, it is additionally provided on housing
For the button switch of control circuit board power supply.
Preferably, the hull outside setting is there are one handle, and described handle one end is fixed on the housing, the other end
It is bent to towards the direction of magnetic pole.By the handle of peripheral hardware on housing, it on the one hand can realize and successfully hang, put biological substance point
From device；On the other hand, it since the handle is bending, is held convenient for user, is highly convenient for using.
Preferably, the electric device is arranged on the enclosure interior, be fixedly connected in the magnetosheath connection ring there are one
Driven gear, there are one the transmission gears engaged with the driven gear for setting on the electric device.Electric device (specifically may be used
It is arranged to a motor, and when magnetosheath is single, is preferably arranged in housing) it is installed with transmission gear, electric device
After operation, transmission gear rotation so that the driven gear engaged also rotates, and finally drives magnetosheath rotating dress
It puts and rotates with magnetosheath；The mode of this kind transmission can realize the rotation of magnetosheath easily, easy to operate feasible.
When the sample disposably handled is more, the mode (magnetosheath is configured in i.e. each housing) of single head obviously cannot expire
Sufficient batch processing demand, it is therefore preferable that multiple magnetosheaths are set, and realized by a main shaft push rod while control multiple magnetosheaths,
Magnetic pole is very important, therefore, it is further preferred that the biological substance separator further includes a bull link；
One end of the bull link and the housing is socketed；
The main shaft push rod includes a drive link and Duo Gen follower levers；All follower levers are arranged side by side, and by a connection
Bar is fixed, and the connecting rod is fixedly connected with the drive link；The drive link, follower lever and connecting rod are arranged at described
On bull link；All follower levers both pass through a fixed plate, and it passes through one end of the fixed plate to be both provided with
Magnetic pole, the fixed plate are fixed on the bull link；Magnetosheath connection ring is arranged on each follower lever；
One end is both provided with magnetosheath in each magnetosheath connection ring, and the other end is respectively and fixedly provided with driven gear；Per two adjacent driven gears
Between intermesh, the electric device is arranged on the bull link, and for the driven gear to be driven to rotate.
In the biological substance separator of above-mentioned " bull structure ", main shaft push rod with it is simple in " single head structure "
Main shaft push rod it is not consistent, in this scenario, main shaft push rod is divided into two parts, wherein the part in housing for transmission
Bar (namely main shaft push rod in " single head structure ")；Hull outside is divided into more follower levers arranged side by side；More drive link quilts
One connecting rod is fixed, and drive link is then fixedly connected with the connecting rod.In this way after set application active force is wrapped up push rod, drive link
Connecting rod and all follower levers is then pushed to move, the magnetic pole for being arranged on each driven boom end at this time is then also pushed and stretched
Enter to the bottom of its corresponding magnetosheath.
Further, since it is arranged with magnetosheath connection ring on each follower lever；One end of each magnetosheath connection ring is both provided with
Magnetosheath, the other end are respectively and fixedly provided with gear；It intermeshes between per two adjacent gears, electric device is arranged on the bull and connects
It connects on frame (convenient to realize that multiple gears rotate simultaneously by an electric device).After electric device is run, as long as driving is multiple
A gear in gear, which rotates, can be realized the rotation of all gear sets.
Due on bull link and the structures such as fixed plate, when main shaft push rod (drive link) moves, only owning
Follower lever (magnetic pole including its end), connecting rod movement, and between gear set, fixed plate and bull link and housing
It is relatively stationary.
The above-mentioned biological substance separator with " bull structure ", corresponding construction and the single head biology of enclosure interior
Material disconnecting gear is basically identical, however multiple magnetosheaths that the biological substance separator of the bull has due to it are (including magnetic
Column), batch processing can be carried out at the same time to the treatment fluid arranged in advance, so as to improve treatment effeciency, be saved for experiment
A large amount of time.
Preferably, the drive link and all follower levers are parallel, and the connecting rod is vertical with the drive link.
Preferably, the magnetosheath is one end open, and the cavity of one end closing, one end of opening is set in the magnetosheath and connects
It connects on ring, one end of closing sets multiple protrusions.
After the multiple protrusions of external setting of one end of magnetosheath closing, one side facilitates magnetic bead to be adsorbed to its table
On the other hand face can also be effectively prevented magnetosheath the magnetic bead assembled during rotation is scattered the phenomenon that.
Preferably, the cecum outer surface of the magnetosheath can be V-arrangement asperities, U-shaped asperities, V-arrangement smooth surface or U-shaped smooth surface.This
Outside, magnetic pole can be cylindrical, square column type, ellipse, pie or any shape that can be placed inside tubular container.
The blind end (cecum) of magnetosheath can be V-arrangement or U-shaped, and to increase mixing effect, cecum can be additional
' linear type ', ' cross ' protrusion.
Magnetosheath can be made of plastics, rubber, glass or not magnetic any known material, can be it is transparent,
Opaque or different colors.Moreover, the connection mode between magnetosheath connection ring and electric device is changeable (being not limited to rotate),
I.e. magnetosheath can do and vibrate.
Compared with prior art, beneficial effects of the present invention are：
(1), this biological substance separator provided by the invention after being applied to biological substance separation, has separation
Thoroughly, efficient advantage；And during being detached using the device, which can greatly simplified operation, gram
The defects of magnetic bead easily is not easy to remove by water remaining in absorption and magnetic bead in the prior art is taken.
(2), the total setting of the device is compact, easy to use, when magnetosheath rotates, by adjusting its rotating speed, goes back
The effect of stirring can be played, there is vortex instrument.
(3), due to the setting of magnetic pole, magnetosheath is in rotary course, and magnetic bead is maintained at by the effect in magnetic field on magnetosheath, and liquid
Body is got rid of de- under the influence of centrifugal force so that magnetic bead and liquid further detach.In addition, the outer surface to magnetosheath is arranged to
After asperities, the effect of grinding can be played to mixed system during rotation, therefore there is tissue, clasmatosis.
Description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described.
Fig. 1, Fig. 2 and Fig. 3 are that multifunctional bio material disconnecting gear provided by the invention shows in the structure of " single head structure "
It is intended to；
Reference numeral：
Housing -101；Main shaft push rod -102；Magnetosheath connection ring -103；Electric device -104；Magnetic pole -105；Magnetosheath -106；
First lantern ring -201；Second lantern ring -202；First spring -203；Wedging position -204；Push rod package set -205；Positioning sleeve-
206；Second spring -207；Magnetosheath is detached from push rod -208；Button switch -209；Handle -210；Driven gear -211；
Fig. 4, Fig. 5 are the structure diagram that the multifunctional bio material disconnecting gear that the present invention supplies is in " bull structure "；
Reference numeral：
Housing -101；Main shaft push rod -102：Magnetosheath connection ring -103；Electric device -104；Magnetosheath -106；Push rod wraps up
Set -205；Positioning sleeve -206；Second spring -207；Magnetosheath is detached from push rod -208；Button switch -209；Handle -210；Driven tooth
Wheel -211；Bull link -301；Follower lever -302；Connecting rod -303；Fixed plate -304；Protrusion -305.
Specific embodiment
- Fig. 3 is please referred to Fig.1, this multifunctional bio material disconnecting gear provided by the invention including housing 101, runs through
Main shaft push rod 102, returning device, magnetosheath connection ring 103 and the electric device 104 of housing 101；The both ends of main shaft push rod 102
Housing 101 is protruded from, and one end is provided with magnetic pole 105 (embodying in fig. 2, similarly hereinafter)；Magnetosheath connection ring 103 (Fig. 1 and figure
It is not shown in 3, corresponding position is embodied in fig. 2, similarly hereinafter) it is sheathed on main shaft push rod 102, and magnetosheath connection ring 103 is in
One end outside housing 101 is provided with magnetosheath 106；Returning device is arranged on inside housing 101；And for make main shaft push rod 102 to
Direction far from magnetosheath 106 moves；Electric device 104 is connected with magnetosheath connection ring 103, and for sending out magnetosheath connection ring 103
Raw rotation.
Above-mentioned biological substance separator, not only can be efficient, rapidly realizes that mixed system (contains magnetic material
Matter, such as magnetic bead) separating effect；It, can be non-but also with the effect of the instruments such as similar vortex instrument, dehydration centrifuge, beveller
Often it is conveniently used in biological experiment.
In more preferred scheme, wedging position 204 is further fixedly arranged in housing 101；Returning device includes first
Lantern ring 201, the second lantern ring 202 and the first spring 203；
First lantern ring 201, the first spring 203 and the second lantern ring 202 are sheathed on respectively on main shaft push rod 102, first set
Ring 201 offsets with wedging position 204, and the second lantern ring 202 is fixedly connected with main shaft push rod 102, the both ends difference of the first spring 203
It offsets with the first lantern ring 201 and the second lantern ring 202；
One end on main shaft push rod 102 outside housing 101 is provided with push rod package set 205；Push rod package 205 envelopes of set
The one end closed is wrapped in the end of main shaft push rod 102, and open one end offsets with the second lantern ring 202.
For wedging position 204, structure is there is no particular determination, as long as being fixed on the inside of housing 101 and can be with
First lantern ring 201 there are larger contact surface, additionally, it is preferred that, in order to play the effect of fixed main shaft push rod 102, card
There are one sliding slot, which passes through for main shaft push rod 102 for the free end setting of socket part position 204, so as to main shaft push rod 102,
First lantern ring 201, second lantern ring 202 etc. play locating effect.In addition, for the first lantern ring 201 and the second lantern ring 202, it is
It realizes its being bonded between the first spring 203, includes for the prominent of sheathed spring (both ends for stretching into spring)
Go out portion.
Returning device with above structure can fast and effeciently realize the return effect of main shaft push rod 102；It is compressed
The first spring 203 can equably force on main shaft push rod 102, so as to promote to return it to original position.
It is smooth in order to further so that main shaft push rod 102 is steady in the process of reciprocating, in the base of said program
On plinth；Further, positioning sleeve 206 is further fixedly arranged on inside housing 101, positioning sleeve 206 is sheathed on push rod package set 205
Part in housing 101；The inner wall of positioning sleeve 206 and the outer wall of push rod package set 205 fit.
In the present invention, since magnetosheath 106 and magnetosheath connection ring 103 are sockets, make when magnetosheath 106 is repeated as many times
With or meet operation the needs of when, generally require to lift down it from magnetosheath connection ring 103；And in order to facilitate itself and magnetosheath
Connection ring 103 is detached from, it is further preferred that on the basis of above-mentioned preferred embodiment, is further included and is arranged on housing 101
Second spring 207 and magnetosheath are detached from push rod 208；One end of second spring 207 is fixed on housing 101, and the other end takes off with magnetosheath
One end from push rod 208 offsets, and the other end that magnetosheath is detached from push rod 208 extends to the main shaft push rod 102 for being equipped with magnetic pole 105
The opening of housing 101 that passes through of one end.
Magnetosheath is detached from push rod 208 under the effect of external force, is moved along housing 101, specially towards being provided with magnetosheath
106 101 end motion of housing, during movement, bottom can be abutted on magnetosheath 106, and then can take off magnetosheath 106
From after magnetosheath 106 is detached from, under the action of second spring 207, magnetosheath is detached from push rod 208 and returns to original position.
The structure of push rod 208 is detached from for magnetosheath, specific restriction is not done, as long as it disclosure satisfy that along housing 101
Reciprocating motion, such as it can be with groove-like structure, the one end for being used to push away magnetosheath 106 is further fixedly arranged on a lantern ring
(in figure 3 embody), lantern ring can be equably by magnetosheath 106 from magnetosheath connection ring 103 in this way when applying thrust to its other end
On push.For second spring 207, one end or part need to be fixed on housing 101, and the other end is then detached from magnetosheath
One end (two ends) of push rod 208 offsets.In addition, in order to enable structure is compacter rationally, the first spring 203 and the
It is parallel between two springs 207.
In further preferred scheme, circuit board is additionally provided in housing 101, and (installation position is random, does not show in figure
Go out)；Circuit board is electrically connected with electric device 104, and the motor adjusting knob for protruding from housing 101 is additionally provided on circuit board
And charge port (not shown in FIG.)；And the button switch 209 for control circuit board power supply is additionally provided on housing 101.
By circuit board, various circuits can be integrated, motor adjusting knob can realize that electric device 104 (is specially one
Motor) output power is adjusted, and has directly controlled magnetosheath connection ring 103 and the slewing rate of magnetosheath 106；Realize magnetosheath 106
Rotational speed regulation；And by charge port (for convenience, can be set to USB interface), it can realize charging effect to power supply；This
Outside, the button switch 209 for control circuit board power supply is additionally provided on housing 101.During use, open power supply and press
Button switch 209, so that electric device 104 enters working condition, and electronic dress can then be adjusted by adjusting motor adjusting knob
Put 104 working efficiency.It should be pointed out that it is random for the installation position of circuit board, and for motor adjusting knob, then it is excellent
Choosing is arranged on the middle part of housing 101 so that using then convenient for adjusting motor power；And button switch 209 is then arranged on housing 101
End, convenient for thumb press operate.
In addition, for the ease of taking, putting the biological substance separator, on the basis of above-mentioned preferred embodiment, further
Preferably, there are one handle 210,210 one end of handle to be fixed on housing 101 setting of the outside of housing 101, and the other end is to towards magnetic
The direction bending of column 105.
Under normal circumstances, a magnetosheath 106 is configured in each biological substance separator, i.e., " single head structure "；This is whole
Body structure is similar to the shape of common liquid-transfering gun, in order to enable its 106 smooth rotation of magnetosheath, in above-mentioned preferred technical side
On the basis of case, electric device 104 is arranged on inside housing 101, is fixedly connected in magnetosheath connection ring 103 there are one driven tooth
Wheel 211, there are one the transmission gears engaged with driven gear 211 for setting on electric device 104.
Incorporated by reference to Fig. 1-Fig. 3, in above-mentioned technical proposal, the structure and its operation principle of single head separator are embodied, so
And during practical operation, when the sample disposably handled is more, mode (i.e. each configuration of housing 101 one of single head
A magnetosheath 106) batch processing demand obviously is unsatisfactory for, it is therefore preferable that setting multiple magnetosheaths 106, and pass through a main shaft push rod
102 realize while multiple magnetosheaths 106, magnetic pole 105 are controlled to be very important.
Therefore ,-Fig. 5 is please referred to Fig.4, the present invention also provides a kind of biological substance separator, including：Housing 101,
Through the main shaft push rod 102 of housing 101, returning device, magnetosheath connection ring 103 and electric device 104；
Main shaft push rod 102 (specifically includes drive link inside housing 101 and outside housing 101 in this scenario
The follower lever 302 in portion, wherein drive link are not shown due to being in inside housing 101 in figure) both ends it is prominent
In housing 101, and one end is provided with magnetic pole 105；Magnetosheath connection ring 103 is sheathed on main shaft push rod 102 (magnetosheath connection ring
103 structure with consistent shown in Fig. 1-Fig. 3, in the present solution, magnetosheath connection ring 103 be specifically arranged on follower lever 302,
Due to being blocked in Figure 4 and 5, do not show that), and one end that magnetosheath connection ring 103 is in outside housing 101 is provided with magnetosheath
106；Returning device is arranged on inside housing 101；And for main shaft push rod 102 to be made to be moved to the direction far from magnetosheath 106；It is electronic
Device 104 is connected with magnetosheath connection ring 103, and for magnetosheath connection ring 103 to be made to rotate；
Positioning sleeve 206 is further fixedly arranged on inside housing 101, positioning sleeve 206 is sheathed on push rod package set 205 in housing
Part in 101；The inner wall of positioning sleeve 206 and the outer wall of push rod package set 205 fit；
The device further includes：The second spring 207 and magnetosheath being arranged on housing 101 are detached from push rod 208；Second spring
207 one end is fixed on housing 101, and one end that the other end is detached from push rod 208 with magnetosheath offsets, and magnetosheath is detached from push rod 208
The other end extends to the opening of housing 101 that the one end for the main shaft push rod 102 for being equipped with magnetic pole 105 passes through；
Circuit board is additionally provided in housing 101；Circuit board is electrically connected with electric device 104, and is additionally provided on circuit board
Protrude from the motor adjusting knob and charge port of housing 101；And it is additionally provided on housing 101 for control circuit board power supply
Button switch 209.
There are one handle 210,210 one end of handle to be fixed on housing 101 setting of the outside of housing 101, the other end to towards
The direction bending of magnetic pole 105；
The biological substance separator further includes a bull link 301；Bull link 301 and the one of housing 101
End cap connects；Main shaft push rod 102 includes a drive link and Duo Gen follower levers 302；All follower levers 302 are arranged side by side, and by one
Connecting rod 303 is fixed, and connecting rod 303 is fixedly connected with drive link；Drive link, follower lever 302 and connecting rod 303 are arranged at
On bull link 301；
All follower levers 302 both pass through a fixed plate 304, and it passes through one end of fixed plate 304 to be both provided with magnetic pole
105, fixed plate 304 is fixed on bull link 301；
Magnetosheath connection ring 103 is arranged on each follower lever 302；In each magnetosheath connection ring 103, one end is both provided with
Magnetosheath 106, the other end is respectively and fixedly provided with gear；It intermeshes between per two adjacent gears, electric device 104 is arranged on bull
On link 301, and rotated for drive gear.
In the biological substance separator of above-mentioned " bull structure ", main shaft push rod 102 is divided to for two parts, wherein locating
In the part in housing 101 be drive link；And 101 outer portion of housing includes the follower lever 302 more side by side；More drive link quilts
One connecting rod 303 is fixed, and drive link is then fixedly connected with connecting rod 303.
After applying active force to push rod package set 205, drive link then pushes connecting rod 303 and all follower levers 302
Movement, the magnetic pole 105 for being arranged on each 302 end of follower lever at this time are then also pushed and stretch to its corresponding magnetosheath 106
Bottom.In addition, due to being arranged with magnetosheath connection ring 103 on each follower lever 302；In each magnetosheath connection ring 103, one end is equal
Magnetosheath 106 is provided with, the other end is respectively and fixedly provided with gear；It intermeshes between per two adjacent gears, (the tool of electric device 104
Body is a motor) it is arranged on bull link 301 (convenient to realize that multiple gears rotate simultaneously by an electric device 104).
After electric device 104 is run, as long as a gear in multiple gears is driven, which to rotate, can be realized all gear sets
Rotation, and then easily realize the rotation of all magnetosheaths 106.
Due to the structures such as bull link 301 and fixed plate 304, (it is specially drive link, is in main shaft push rod 102
Inside housing 101, in Fig. 5) movement when, only all follower levers 302 (magnetic pole 105 including its end), connecting rod 303
Movement, and be relatively stationary between gear set, fixed plate 304 and bull link 301 and housing 101.
The above-mentioned biological substance separator with bull structure, the corresponding construction inside housing 101 are given birth to single head
Object material disconnecting gear is basically identical, however (the packet of multiple magnetosheaths 106 that the biological substance separator of the bull has due to it
Include magnetic pole 105), batch processing can be carried out at the same time to the treatment fluid arranged in advance, so as to improve treatment effeciency.
It is further preferred that drive link and all follower levers 302 are parallel, connecting rod 303 is vertical with drive link；Magnetosheath
106 be one end open, and the cavity of one end closing, one end of opening is set in magnetosheath connection ring 103, one end setting of closing
Multiple raised 305, the structure of protrusion 305 is specially fan blade, to increase stirring and vortex work(of the magnetosheath 106 during rotation
Energy.
This biological substance separator provided by the invention opens a novel way for biological substance separation field
Diameter；By taking its quick separating DNA as an example, step only has four steps, and the time was in completion in one minute or so；
Specific steps：
1. adding in magnetic bead, lysate and peripheral blood in the first pipe, start rotation button, accelerate to rotate with device, cell
It can be cleaved in moment, DNA is discharged into lysate；
2. pressing the button that stops rotating, main shaft push rod is pushed to provide magnetic field, magnetic bead is inhaled under the action of magnetic pole magnetic force
Magnetosheath periphery；
3. the magnetic bead with DNA is cleaned in washing lotion；
Come 4. DNA and magnetic bead are separated in eluent, then magnetic field is provided, magnetic bead is picked up, and throws aside magnetic bead i.e.
The DNA that can be purified.
As it can be seen that by the device, the step of biological substance detaches is greatly simplified, sets up extraction DNA speed
Milestone (1 minute extraction DNA), creates the single instrument of biological substance separation field and completes multiple function precedents；Specifically, its
Create the new method of dehydrating function in separate substance engineering；It sets up microminiature instrument and solves the problems, such as huge model；It establishes
The new tool that liquid is mixed in substance separation process；The ingenious combination of the Design and Features of magnetosheath is embodied (in magnetic field
In the presence of round-trip and rotary motion) value；Embodying increases the function of grinding and disrupting tissue after magnetosheath shape changes.
To sum up, multifunctional bio material disconnecting gear provided by the invention is various big quickly to be removed from liquid media
The magnetic-particle of small and shape and quick, light, quick, the moveable new instrument of one kind designed.
The device from contaminated liquids suitable for removing pollutant or other impurity.Such as from blood, marrow, brain ridge
In liquid, cell culture medium, food, milk, beverage remove and purify virus, bacterium, toxin, hormone, drug, insecticide, itself
Immune protein, antigen, haptens etc..Also unwanted impurity can be removed from biological reagent, iundustrial oil, chemical liquid.
Field of medical biology is mainly used for quick separating biological substance.It is common to have from different types of animal or plant tissue soon
Speed separation nucleic acid, protein, virus and various cells；It can also be used for removing internal harmful virus (such as hepatitis, AIDS
Disease), cancer cell (such as leukaemia), autoimmunity albumen (such as systemic loupus erythematosus, rheumatoid arthritis) and play and control
Treat and alleviate the purpose of disease.
The multifunctional bio material disconnecting gear medicine, field of biology biological substance separation in terms of, either it
Separating rate, accuracy, agility all surmounted current Routine Test Lab overwhelming majority instrument.The dexterous, function of its body
Assembly, using convenience, operation it is it is simple, set up the mileage that single type in world's similar product completes multiple functions
Upright stone tablet；Multifunctional bio material disconnecting gear is in environmental protection and energy saving, safe and harmless aspect and the speed and essence in biological substance separation
The first in the world of similar product is all created in terms of accuracy.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from the present invention's
Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of multifunctional bio material disconnecting gear, which is characterized in that including housing, through the housing main shaft push rod,
Returning device, magnetosheath connection ring and electric device；
The both ends of the main shaft push rod protrude from the housing, and one end is provided with magnetic pole；The magnetosheath connection ring is arranged
In on the main shaft push rod, and one end that the magnetosheath connection ring is in outside the housing is provided with magnetosheath；
The returning device is arranged on the enclosure interior；And for the main shaft push rod to be made to be transported to the direction far from the magnetosheath
It is dynamic；The electric device is connected with the magnetosheath connection ring, and for the magnetosheath connection ring to be made to rotate.
2. multifunctional bio material disconnecting gear according to claim 1, which is characterized in that also fixation is set in the housing
It is equipped with wedging position；The returning device includes the first lantern ring, the second lantern ring and the first spring；
First lantern ring, the first spring and the second lantern ring are sheathed on respectively on the main shaft push rod, first lantern ring with
The wedging position offsets, and second lantern ring is fixedly connected with the main shaft push rod, the both ends of first spring respectively with
First lantern ring and second lantern ring offset；
One end in the hull outside is provided with push rod package set on the main shaft push rod；The push rod package set closing
One end is wrapped in the end of the main shaft push rod, and open one end offsets with second lantern ring.
3. multifunctional bio material disconnecting gear according to claim 2, which is characterized in that the enclosure interior is also fixed
Positioning sleeve is provided with, the positioning sleeve is sheathed on part of the push rod package set in the housing；The positioning sleeve
The outer wall of inner wall and push rod package set fits.
4. multifunctional bio material disconnecting gear according to claim 3, which is characterized in that further include and be arranged on the shell
Second spring and magnetosheath on body are detached from push rod；One end of the second spring is fixed on the housing, the other end with it is described
One end that magnetosheath is detached from push rod offsets, and the other end that the magnetosheath is detached from push rod extends to opening for the housing that is passed through by magnetic pole
At mouthful.
5. multifunctional bio material disconnecting gear according to claim 4, which is characterized in that be additionally provided in the housing
Circuit board；The circuit board is electrically connected with the electric device, and is additionally provided on the circuit board and is protruded from the housing
Motor adjusting knob and charge port；And it is additionally provided on the housing for the button switch of control circuit board power supply.
6. multifunctional bio material disconnecting gear according to claim 5, which is characterized in that the hull outside is provided with
One handle, described handle one end are fixed on the housing, and the other end is bent to towards the direction of magnetic pole.
7. multifunctional bio material disconnecting gear according to claim 6, which is characterized in that the electric device is arranged on
The enclosure interior is fixedly connected in the magnetosheath connection ring there are one driven gear, on the electric device there are one settings
The transmission gear engaged with the driven gear.
8. multifunctional bio material disconnecting gear according to claim 6, which is characterized in that the biological substance separation dress
It puts and further includes a bull link；One end of the bull link and the housing is socketed；
The main shaft push rod includes a drive link and Duo Gen follower levers；All follower levers are arranged side by side, and are consolidated by a connecting rod
Fixed, the connecting rod is fixedly connected with the drive link；The drive link, follower lever and connecting rod are arranged at the bull
On link；
All follower levers both pass through a fixed plate, and it passes through one end of the fixed plate to be both provided with magnetic pole, described
Fixed plate is fixed on the bull link；
Magnetosheath connection ring is arranged on each follower lever；One end of each magnetosheath connection ring is both provided with magnetosheath, another
End is respectively and fixedly provided with driven gear；It intermeshes between per two adjacent driven gears, the electric device is arranged on described more
On head link, and for the driven gear to be driven to rotate.
9. multifunctional bio material disconnecting gear according to claim 8, which is characterized in that the drive link and all institutes
It is parallel to state follower lever, the connecting rod is vertical with the drive link.
10. according to claim 1-9 any one of them multifunctional bio material disconnecting gears, which is characterized in that the magnetosheath
For one end open, the cavity of one end closing, one end of opening is set in the magnetosheath connection ring, and one end setting of closing is more
A protrusion.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610034026.7A CN105420074B (en) | 2016-01-19 | 2016-01-19 | A kind of multifunctional bio material disconnecting gear |
| EP16890137.9A EP3405288A4 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| PCT/CA2016/000204 WO2017139864A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| CA3008660A CA3008660A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| US16/071,038 US20190022667A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610034026.7A CN105420074B (en) | 2016-01-19 | 2016-01-19 | A kind of multifunctional bio material disconnecting gear |
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| Publication Number | Publication Date |
|---|---|
| CN105420074A CN105420074A (en) | 2016-03-23 |
| CN105420074B true CN105420074B (en) | 2018-06-29 |
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| CN201610034026.7A Expired - Fee Related CN105420074B (en) | 2016-01-19 | 2016-01-19 | A kind of multifunctional bio material disconnecting gear |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20180090543A (en) * | 2017-02-03 | 2018-08-13 | 주식회사 미코바이오메드 | Apparatus and Process For Isolating or Purifying Target Material From Biological Sample |
| CN108330097B (en) * | 2018-03-16 | 2020-10-23 | 天信和(苏州)生物科技有限公司 | Cell culture and detection method |
| CN108220126A (en) * | 2018-03-21 | 2018-06-29 | 山西振东基赛生物科技有限公司 | Bio-carrier device |
| CN108772358A (en) * | 2018-04-27 | 2018-11-09 | 泰州泽成生物技术有限公司 | A kind of new automatic more reagent strip cleaning magnetic beads mechanism |
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| CN105420074A (en) | 2016-03-23 |
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Effective date of registration: 20170216 Address after: 030600 Jinzhong Development Zone, Shanxi Hui Road, No. 8 Applicant after: SHANXI ZDGSY BIO-SCIENTIFIC CO., LTD. Applicant after: Shanxi Traditional Chinese Medical College Address before: 030600 Jinzhong Economic Development Zone, Shanxi Road No. 8 Applicant before: SHANXI ZDGSY BIO-SCIENTIFIC CO., LTD. |
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