CN105400680B - A kind of quantitative real time PCR Instrument - Google Patents
A kind of quantitative real time PCR Instrument Download PDFInfo
- Publication number
- CN105400680B CN105400680B CN201510911542.9A CN201510911542A CN105400680B CN 105400680 B CN105400680 B CN 105400680B CN 201510911542 A CN201510911542 A CN 201510911542A CN 105400680 B CN105400680 B CN 105400680B
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- Prior art keywords
- gear
- pcr instrument
- bearing
- motor
- main frame
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
Abstract
A kind of quantitative real time PCR Instrument, belong to technical field of medical equipment, including kit, upper plate, elastic rod, spring, support plate, first gear, roller bearing, flange bearing, controller, bottom plate, second motor, support frame, axle sleeve, second gear, second bearing pin, connecting rod, flake bearing, connecting pole, first bearing pin, cylinder, slide rail, connecting rod, leading screw, sliding block, screw, bearing bracket stand, first motor, support, PCR instrument main frame and cover plate, mounting flange bearing on the bottom plate, roller bearing is installed on flange bearing, first gear is installed on roller bearing, roller bearing upper end is arranged under support plate, first gear each side installs second gear, second gear is mutually coincide with first gear, second gear is arranged on axle sleeve, axle sleeve is arranged on the output shaft of the second motor；It is an advantage of the invention that：Kit can be automatically turned on.
Description
Technical field
The present invention relates to a kind of quantitative real time PCR Instrument, belong to technical field of medical equipment.
Background technology
Quantitative real time PCR Instrument is specific target gene detection and quantitative unified platform, by PCR thermal cycles, fluorescence inspection
Survey and various analysis softwares be combined together, can dynamically observe in each reaction tube of each circulations of PCR pcr amplification product by
Gradually increased situation.The kit of the quantitative real time PCR Instrument of prior art is required for people to pull out manually, easily makes on kit
It is infected with germs, and then influences experimental analysis.In order to solve above-mentioned difficulties, it is necessary to which developing a can automatically turn on the new of kit
Quantitative real time PCR Instrument.
The content of the invention
It is an object of the invention to provide a kind of quantitative real time PCR Instrument.
The problem to be solved in the present invention is that the kit of the quantitative real time PCR Instrument of prior art is required for people to pull out manually
The problem of.
To realize the purpose of the present invention, the technical solution adopted by the present invention is：
A kind of quantitative real time PCR Instrument, including kit, upper plate, elastic rod, spring, support plate, first gear, roller bearing, flange
Bearing, controller, bottom plate, the second motor, support frame, axle sleeve, second gear, the second bearing pin, connecting rod, flake bearing, connecting pole,
First bearing pin, cylinder, slide rail, connecting rod, leading screw, sliding block, screw, bearing bracket stand, the first motor, support, PCR instrument main frame and lid
Plate, mounting flange bearing on the bottom plate, roller bearing is installed on flange bearing, first gear, the installation of roller bearing upper end are installed on roller bearing
Under support plate, first gear each side installs second gear, and second gear is mutually coincide with first gear, second gear peace
On axle sleeve, axle sleeve is arranged on the output shaft of the second motor, and the second motor is arranged on bottom plate, the installation support of axle sleeve lower end
Frame, support frame are arranged on bottom plate, and controller is arranged on bottom plate, and controller is connected by wire with the second motor, on support plate
More than one spring and more than one elastic rod are installed, upper plate is installed on spring and elastic rod, PCR instrument main frame is installed on upper plate,
Installation kit, kit each side install flake bearing, connecting pole, cylinder are installed on flake bearing in PCR instrument main frame
Piston rod be arranged on the right side of connecting pole, cylinder is arranged on PCR instrument main frame side, and kit each side installs the first pin
Axle, connecting rod are arranged on the first bearing pin, and connecting rod lower end is arranged on PCR instrument main frame side, PCR instrument main frame or so by the second bearing pin
Slide rail is respectively installed in both sides, and sliding block is installed on slide rail, connecting rod is installed on the left of sliding block, cover plate, cover plate are installed between two connecting rods
Kit is covered, screw is installed on the right side of sliding block, screw is arranged on leading screw, and leading screw upper end passes through shaft coupling and the first motor phase
Even, the first motor is arranged on support, and the first motor is connected by wire with controller, and support is arranged on PCR instrument main frame, silk
Thick stick left and right ends are respectively arranged on bearing bracket stand, and bearing bracket stand is arranged on slide rail.
The cylinder internal solenoid valve, magnetic valve are connected by wire with controller.
It is an advantage of the invention that：Controller controls the second electric motor starting, rotates second gear, drives first gear rotation
Turn, support plate can be rotated, rotate PCR instrument main frame, to change the direction of PCR instrument main frame；Spring and elastic rod are avoided that PCR
Instrument main frame produced when being knocked it is larger rock, preserve PCR instrument main frame steady；Controller controls the first electric motor starting, makes
Leading screw rotates, and moves up screw, moves up sliding block, move up cover plate, exposes kit；Controller control
The energization or power-off of cylinder internal solenoid valve processed, make the piston rod of cylinder do stretching routine, are displaced outwardly kit, avoid people
It is manually pulled out kit.
Brief description of the drawings
Fig. 1 is a kind of quantitative real time PCR Instrument overall structure front view of the present invention；
Fig. 2 is the left view of a portions structure；
In figure：1st, kit 2, upper plate 3, elastic rod 4, spring 5, support plate 6, first gear 7, roller bearing
8th, flange bearing 9, controller 10, bottom plate 11, the second motor 12, support frame 13, axle sleeve 14, second gear
15th, the second bearing pin 16, connecting rod 17, flake bearing 18, connecting pole 19, the first bearing pin 20, cylinder 21, slide rail
22nd, connecting rod 23, leading screw 24, sliding block 25, screw 26, bearing bracket stand 27, the first motor 28, support 29, PCR
Instrument main frame 30, cover plate.
Embodiment
Below in conjunction with the accompanying drawings and embodiment the present invention is further illustrated.
A kind of quantitative real time PCR Instrument of the present invention, including kit 1, upper plate 2, elastic rod 3, spring 4, support plate 5, the first tooth
Wheel 6, roller bearing 7, flange bearing 8, controller 9, bottom plate 10, the second motor 11, support frame 12, axle sleeve 13, second gear 14, second
Bearing pin 15, connecting rod 16, flake bearing 17, connecting pole 18, the first bearing pin 19, cylinder 20, slide rail 21, connecting rod 22, leading screw 23, cunning
Block 24, screw 25, bearing bracket stand 26, the first motor 27, support 28, PCR instrument main frame 29 and cover plate 30, Method for Installation on the bottom plate 10
Blue bearing 8, roller bearing 7 to be installed on flange bearing 8, first gear 6 is installed on roller bearing 7, the upper end of roller bearing 7 is arranged under support plate 5, and first
Gear 6 each side installs second gear 14, and second gear 14 is mutually coincide with first gear 6, and second gear 14 is arranged on
On axle sleeve 13, axle sleeve 13 is arranged on the output shaft of the second motor 11, and the second motor 11 is arranged on bottom plate 10, and controller 9 is controlled
Make the second motor 11 to start, rotate second gear 14, drive first gear 6 to rotate, can rotate support plate 5, make PCR instrument master
Machine 29 rotates, to change the direction of PCR instrument main frame 29；Support frame 12 is installed in the lower end of axle sleeve 13, and support frame 12 is arranged on bottom plate 10
On, controller 9 is arranged on bottom plate 10, and controller 9 is connected by wire with the second motor 11, and more than one is installed on support plate 5
Upper plate 2 is installed, spring 4 and elastic rod 3 are avoided that PCR instrument master on spring 4 and more than one elastic rod 3, spring 4 and elastic rod 3
Machine 29 produced when being knocked it is larger rock, PCR instrument main frame 29 is preserved steadily；PCR instrument main frame 29, PCR is installed on upper plate 2
Installation kit 1, kit 1 each side install flake bearing 17, connecting pole are installed on flake bearing 17 in instrument main frame 29
18, the piston rod of cylinder 20 is arranged on the right side of connecting pole 18, and cylinder 20 is arranged on the side of PCR instrument main frame 29, the in-built electrical of cylinder 20
Magnet valve, magnetic valve are connected by wire with controller 9, and kit 1 each side installs the first bearing pin 19, and connecting rod 16 is arranged on
On first bearing pin 19, the lower end of connecting rod 16 is arranged on the side of PCR instrument main frame 29 by the second bearing pin 15, and controller 9 controls cylinder 20
The energization or power-off of internal solenoid valve, make the piston rod of cylinder 20 do stretching routine, are displaced outwardly kit 1, avoid human hand
It is dynamic to pull out kit 1；PCR instrument main frame 29 each side installs slide rail 21, and sliding block 24 is installed on slide rail 21, and the left side of sliding block 24 is pacified
Load extension bar 22, installs cover plate 30 between two connecting rods 22, and cover plate 30 covers kit 1, the right side of sliding block 24 installation screw 25,
Screw 25 is arranged on leading screw 23, and the upper end of leading screw 23 is connected by shaft coupling with the first motor 27, and the first motor 27 is arranged on machine
On seat 28, the first motor 27 controls the first motor 27 to start by wire and the connected controllers 9 of controller 9, rotates leading screw 23,
Screw 25 is moved up, moves up sliding block 24, moves up cover plate 30, exposes kit 1；Support 28 is installed
On PCR instrument main frame 29, the left and right ends of leading screw 23 are respectively arranged on bearing bracket stand 26, and bearing bracket stand 26 is arranged on slide rail 21.
Application method of the present invention：Controller 9 controls the second motor 11 to start, and rotates second gear 14, drives the first tooth
The rotation of wheel 6, can rotate support plate 5, rotate PCR instrument main frame 29, to change the direction of PCR instrument main frame 29；Spring 4 and elastic force
Rod 3 be avoided that PCR instrument main frame 29 produced when being knocked it is larger rock, PCR instrument main frame 29 is preserved steadily；Controller 9 is controlled
Make the first motor 27 to start, rotate leading screw 23, move up screw 25, move up sliding block 24, make cover plate 30 upward
It is mobile, expose kit 1；Controller 9 controls the energization or power-off of the internal solenoid valve of cylinder 20, makes the piston rod of cylinder 20
Stretching routine is done, is displaced outwardly kit 1, avoids people from being manually pulled out kit 1.
Claims (2)
1. a kind of quantitative real time PCR Instrument, including kit（1）, upper plate（2）, elastic rod（3）, spring（4）, support plate（5）, first
Gear（6）, roller bearing（7）, flange bearing（8）, controller（9）, bottom plate（10）, the second motor（11）, support frame（12）, axle sleeve
（13）, second gear（14）, the second bearing pin（15）, connecting rod（16）, flake bearing（17）, connecting pole（18）, the first bearing pin（19）、
Cylinder（20）, slide rail（21）, connecting rod（22）, leading screw（23）, sliding block（24）, screw（25）, bearing bracket stand（26）, the first motor
（27）, support（28）, PCR instrument main frame（29）And cover plate（30）, it is characterized in that：The bottom plate（10）Upper mounting flange bearing（8）,
Flange bearing（8）Upper installation roller bearing（7）, roller bearing（7）Upper installation first gear（6）, roller bearing（7）Upper end is arranged on support plate（5）Under,
First gear（6）Second gear is each side installed（14）, second gear（14）With first gear（6）Mutually it coincide, second
Gear（14）Installed in axle sleeve（13）On, axle sleeve（13）Installed in the second motor（11）Output shaft on, the second motor（11）Peace
Mounted in bottom plate（10）On, axle sleeve（13）Lower end is installed by support frame（12）, support frame（12）Installed in bottom plate（10）On, controller
（9）Installed in bottom plate（10）On, controller（9）Pass through wire and the second motor（11）It is connected, support plate（5）Upper installation more than one
Spring（4）With more than one elastic rod（3）, spring（4）And elastic rod（3）Upper installation upper plate（2）, upper plate（2）Upper installation PCR instrument
Main frame（29）, PCR instrument main frame（29）Interior installation kit（1）, kit（1）Flake bearing is each side installed（17）, fish
Axis oculi is held（17）Upper installation connecting pole（18）, cylinder（20）Piston rod be arranged on connecting pole（18）Right side, cylinder（20）Installation
In PCR instrument main frame（29）Side, kit（1）First bearing pin is each side installed（19）, connecting rod（16）Installed in the first pin
Axle（19）On, connecting rod（16）Lower end passes through the second bearing pin（15）Installed in PCR instrument main frame（29）Side, PCR instrument main frame（29）It is left
Right both sides are respectively installed by slide rail（21）, slide rail（21）Upper installation sliding block（24）, sliding block（24）Left side is installed by connecting rod（22）, two companies
Extension bar（22）Between cover plate is installed（30）, cover plate（30）Cover kit（1）, sliding block（24）Right side is installed by screw（25）, screw
（25）Installed in leading screw（23）On, leading screw（23）Upper end passes through shaft coupling and the first motor（27）It is connected, the first motor（27）Peace
Mounted in support（28）On, the first motor（27）Pass through wire and controller（9）It is connected, support（28）Installed in PCR instrument main frame
（29）On, leading screw（23）Left and right ends are respectively arranged on bearing bracket stand（26）On, bearing bracket stand（26）Installed in slide rail（21）On, controller
（9）Control the second motor（11）Start, make second gear（14）Rotation, drive first gear（6）Rotation, can make support plate（5）
Rotation, makes PCR instrument main frame（29）Rotation, to change PCR instrument main frame（29）Direction；Spring（4）And elastic rod（3）It is avoided that
PCR instrument main frame（29）Produced when being knocked it is larger rock, make PCR instrument main frame（29）Preserve steady；Controller（9）Control the
One motor（27）Start, make leading screw（23）Rotation, makes screw（25）Move up, make sliding block（24）Move up, make cover plate
（30）Move up, make kit（1）Expose；Controller（9）Control cylinder（20）The energization or power-off of internal solenoid valve, make
Cylinder（20）Piston rod do stretching routine, make kit（1）It is displaced outwardly, avoids people from being manually pulled out kit（1）.
2. a kind of quantitative real time PCR Instrument according to claim 1, it is characterized in that：The cylinder（20）Internal solenoid valve, electricity
Magnet valve passes through wire and controller（9）It is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510911542.9A CN105400680B (en) | 2015-12-11 | 2015-12-11 | A kind of quantitative real time PCR Instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510911542.9A CN105400680B (en) | 2015-12-11 | 2015-12-11 | A kind of quantitative real time PCR Instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105400680A CN105400680A (en) | 2016-03-16 |
| CN105400680B true CN105400680B (en) | 2017-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510911542.9A Active CN105400680B (en) | 2015-12-11 | 2015-12-11 | A kind of quantitative real time PCR Instrument |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105936869B (en) * | 2016-06-23 | 2018-05-25 | 浙江爱易生物医学科技有限公司 | A kind of Multifunction fluorescent quantitative PCR apparatus |
| CN106047683B (en) * | 2016-06-23 | 2018-08-03 | 浙江爱易生物医学科技有限公司 | A kind of complete set of equipments of genetic test |
| CN106119075B (en) * | 2016-06-23 | 2018-09-04 | 浙江爱易生物医学科技有限公司 | A kind of real-time fluorescence quantitative PCR system |
| CN105969638B (en) * | 2016-06-23 | 2018-11-30 | 浙江爱易生物医学科技有限公司 | A kind of adjustable fluorescence quantitative PCR instrument |
| CN106318863B (en) * | 2016-10-14 | 2018-10-09 | 上海爱易生物医学科技股份有限公司 | A kind of system of genetic test |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6660228B1 (en) * | 1998-03-02 | 2003-12-09 | Cepheid | Apparatus for performing heat-exchanging, chemical reactions |
| KR101420094B1 (en) * | 2010-10-27 | 2014-07-17 | (주)바이오니아 | Automatic realtime PCR system for the various analysis of biological sample, method for Automatic nucleic acid purification and realtime quantification of gene amplification, method for automatic viable cell count of pathogenic bacteria using realtime quantitative PCR, method for automatically getting antigene density using quantitative immunity PCR |
| CN102492603B (en) * | 2011-11-22 | 2013-05-01 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| KR101762295B1 (en) * | 2012-02-10 | 2017-08-04 | (주)바이오니아 | Automatic analysis apparatus and method of biological samples |
| CN105039155B (en) * | 2015-07-29 | 2017-01-25 | 杭州安杰思生物科技有限公司 | Real-time fluorescent quantitative PCR instrument |
| CN205205125U (en) * | 2015-12-11 | 2016-05-04 | 舟山医院 | Novel fluorescent quantitation PCR appearance |
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| CN105400680A (en) | 2016-03-16 |
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Legal Events
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| C06 | Publication | ||
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| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220414 Address after: 102600 Room 403, unit 1, 15th floor, changfengyuan, Huangcun Town, Daxing District, Beijing Patentee after: Du Lihong Address before: 316021 No. 739, dingshen Road, Lincheng street, Dinghai District, Zhoushan City, Zhejiang Province Patentee before: ZHOUSHAN Hospital |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220711 Address after: 300000 Tianjin International Biomedical Joint Research Institute n1012, No. 220 Dongting Road, economic and Technological Development Zone, Binhai New Area, Tianjin Patentee after: TIANJIN MOGENETICS BIOTECHNOLOGY CO.,LTD. Address before: 102600 Room 403, unit 1, 15th floor, changfengyuan, Huangcun Town, Daxing District, Beijing Patentee before: Du Lihong |
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Effective date of registration: 20220824 Address after: Room D209, 2nd Floor, Building 1, No. 12, Shangdi Information Road, Haidian District, Beijing 100000 Patentee after: BEIJING FIRE GENE DX TECHNOLOGY CO.,LTD. Address before: 300000 Tianjin International Biomedical Joint Research Institute n1012, No. 220 Dongting Road, economic and Technological Development Zone, Binhai New Area, Tianjin Patentee before: TIANJIN MOGENETICS BIOTECHNOLOGY CO.,LTD. |