CN105400680A - A novel fluorescent quantitative PCR instrument - Google Patents
A novel fluorescent quantitative PCR instrument Download PDFInfo
- Publication number
- CN105400680A CN105400680A CN201510911542.9A CN201510911542A CN105400680A CN 105400680 A CN105400680 A CN 105400680A CN 201510911542 A CN201510911542 A CN 201510911542A CN 105400680 A CN105400680 A CN 105400680A
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- pcr instrument
- gear
- bearing
- motor
- main frame
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Accommodation For Nursing Or Treatment Tables (AREA)
Abstract
The invention relates to a novel fluorescent quantitative PCR instrument, and belongs to the technical field of medical equipment. The fluorescent quantitative PCR instrument comprises a kit, an upper plate, elastic rods, springs, a supporting plate, a first gear, a roller, a flange bearing, a controller, a base plate, second motors, supports, shaft sleeves, second gears, second pin rolls, connecting rods, fisheye bearings, connecting posts, first pin rolls, air cylinders, sliding rails, connecting bars, lead screws, sliding blocks, nuts, a bearing support, first motors, motor bases, a PCR instrument main machine and a cover plate. The flange bearing is mounted on the base plate. The roller is mounted on the flange bearing. The first gear is mounted on the roller. The upper end of the roller is mounted to the lower part of the supporting plate. The second gearings are disposed at the right and left sides of the first gear respectively and are meshed with the first gear. The second gears are mounted to the shaft sleeves. The shaft sleeves are mounted on output shafts of the second motors. The fluorescent quantitative PCR instrument is advantageous in that the kit can be automatically opened.
Description
Technical field
The present invention relates to a kind of novel fluorescence quantitative PCR instrument, belong to technical field of medical equipment.
Background technology
Quantitative real time PCR Instrument is that specific target gene detects and quantitative unified platform, and by PCR thermal cycling, fluoroscopic examination and various analysis software combine, and dynamically can observe the situation that in each each reaction tubes that circulates of PCR, pcr amplification product increases gradually.The test kit of the quantitative real time PCR Instrument of prior art all needs people manually to pull out, and easily makes test kit is infected with germs, and then affects experimental analysis.In order to solve above-mentioned difficulties, need to develop a novel fluorescence quantitative PCR instrument automatically opening test kit.
Summary of the invention
The object of this invention is to provide a kind of novel fluorescence quantitative PCR instrument.
The problem to be solved in the present invention is the problem that the test kit of the quantitative real time PCR Instrument of prior art all needs people manually to pull out.
For realizing object of the present invention, the technical solution used in the present invention is:
A kind of novel fluorescence quantitative PCR instrument, comprises test kit, upper plate, elastic rod, spring, support plate, first gear, roller bearing, flange bearing, controller, base plate, second motor, bracing frame, axle sleeve, second gear, second bearing pin, connecting rod, flake bearing, joint pin, first bearing pin, cylinder, slide rail, union lever, leading screw, slide block, screw, bottom bracket, first motor, support, PCR instrument main frame and cover plate, mounting flange bearing on described base plate, flange bearing installs roller bearing, roller bearing is installed the first gear, under roller bearing upper end is arranged on support plate, the second gear each side installed by first gear, second gear and the first gear coincide mutually, second gear is arranged on axle sleeve, axle sleeve is arranged on the output shaft of the second motor, second motor is arranged on base plate, bracing frame is installed in axle sleeve lower end, bracing frame is arranged on base plate, controller is arranged on base plate, controller is connected with the second motor by wire, support plate is installed a more than spring and a more than elastic rod, spring and elastic rod install upper plate, upper plate is installed PCR instrument main frame, in PCR instrument main frame, test kit is installed, test kit each side installs flake bearing, joint pin installed by flake bearing, the piston rod of cylinder is arranged on the right side of joint pin, cylinder is arranged on PCR instrument main frame side, test kit each side installs the first bearing pin, connecting rod is arranged on the first bearing pin, connecting rod lower end is arranged on PCR instrument main frame side by the second bearing pin, PCR instrument main frame each side installs slide rail, slide block installed by slide rail, on the left of slide block, union lever is installed, between two union levers, cover plate is installed, cover plate covers test kit, on the right side of slide block, screw is installed, screw is arranged on leading screw, leading screw upper end is connected with the first motor by shaft coupling, first motor is arranged on support, first motor is connected with controller by wire, support is arranged on PCR instrument main frame, two ends, leading screw left and right are respectively arranged on bottom bracket, bottom bracket is arranged on slide rail.
Described cylinder internal solenoid valve, magnetic valve is connected with controller by wire.
Advantage of the present invention is: controller controls the second electric motor starting, make second gear rotate, drive first gear rotate, support plate can be made to rotate, PCR instrument main frame is rotated, change PCR instrument main frame towards; Spring and elastic rod can avoid PCR instrument main frame to produce larger rocking when being knocked, and make PCR instrument main frame preserve steadily; Controller controls the first electric motor starting, and leading screw is rotated, and screw is moved up, and slide block is moved up, and cover plate is moved up, and test kit is exposed; The energising of controller control cylinder internal solenoid valve or power-off, make the piston rod of cylinder do stretching, makes test kit outwards movement, avoid people manually to pull out test kit.
Accompanying drawing explanation
Fig. 1 is a kind of novel fluorescence quantitative PCR instrument of the present invention one-piece construction front view;
Fig. 2 is the left view of a bilge construction;
In figure: 1, test kit 2, upper plate 3, elastic rod 4, spring 5, support plate 6, first gear 7, roller bearing 8, flange bearing 9, controller 10, base plate 11, second motor 12, bracing frame 13, axle sleeve 14, second gear 15, second bearing pin 16, connecting rod 17, flake bearing 18, joint pin 19, first bearing pin 20, cylinder 21, slide rail 22, union lever 23, leading screw 24, slide block 25, screw 26, bottom bracket 27, first motor 28, support 29, PCR instrument main frame 30, cover plate.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further illustrated.
A kind of novel fluorescence quantitative PCR instrument of the present invention, comprises test kit 1, upper plate 2, elastic rod 3, spring 4, support plate 5, first gear 6, roller bearing 7, flange bearing 8, controller 9, base plate 10, second motor 11, bracing frame 12, axle sleeve 13, second gear 14, second bearing pin 15, connecting rod 16, flake bearing 17, joint pin 18, first bearing pin 19, cylinder 20, slide rail 21, union lever 22, leading screw 23, slide block 24, screw 25, bottom bracket 26, first motor 27, support 28, PCR instrument main frame 29 and cover plate 30, mounting flange bearing 8 on described base plate 10, flange bearing 8 is installed roller bearing 7, roller bearing 7 is installed the first gear 6, roller bearing 7 upper end is arranged on support plate 5 times, the second gear 14, second gear 14 each side installed by first gear 6 and the first gear 6 coincide mutually, and the second gear 14 is arranged on axle sleeve 13, axle sleeve 13 is arranged on the output shaft of the second motor 11, second motor 11 is arranged on base plate 10, and controller 9 controls the second motor 11 and starts, and the second gear 14 is rotated, the first gear 6 is driven to rotate, support plate 5 can be made to rotate, PCR instrument main frame 29 is rotated, change PCR instrument main frame 29 towards, bracing frame 12 is installed in axle sleeve 13 lower end, bracing frame 12 is arranged on base plate 10, controller 9 is arranged on base plate 10, controller 9 is connected with the second motor 11 by wire, support plate 5 is installed a more than spring 4 and a more than elastic rod 3, spring 4 and elastic rod 3 install upper plate 2, and spring 4 and elastic rod 3 can avoid PCR instrument main frame 29 to produce larger rocking when being knocked, and make PCR instrument main frame 29 preserve steadily, upper plate 2 is installed PCR instrument main frame 29, in PCR instrument main frame 29, test kit 1 is installed, test kit 1 each side installs flake bearing 17, flake bearing 17 is installed joint pin 18, the piston rod of cylinder 20 is arranged on the right side of joint pin 18, cylinder 20 is arranged on PCR instrument main frame 29 side, cylinder 20 internal solenoid valve, magnetic valve is connected with controller 9 by wire, test kit 1 each side installs the first bearing pin 19, connecting rod 16 is arranged on the first bearing pin 19, connecting rod 16 lower end is arranged on PCR instrument main frame 29 side by the second bearing pin 15, the energising of controller 9 control cylinder 20 internal solenoid valve or power-off, the piston rod of cylinder 20 is made to do stretching, make test kit 1 outwards movement, people is avoided manually to pull out test kit 1, PCR instrument main frame 29 each side installs slide rail 21, slide rail 21 is installed slide block 24, union lever 22 is installed on the left of slide block 24, between two union levers 22, cover plate 30 is installed, cover plate 30 covers test kit 1, screw 25 is installed on the right side of slide block 24, screw 25 is arranged on leading screw 23, leading screw 23 upper end is connected with the first motor 27 by shaft coupling, first motor 27 is arranged on support 28, first motor 27 controls the first motor 27 by wire and controller 9 connected controllers 9 and starts, leading screw 23 is rotated, screw 25 is moved up, slide block 24 is moved up, cover plate 30 is moved up, test kit 1 is exposed, support 28 is arranged on PCR instrument main frame 29, and leading screw about 23 two ends are respectively arranged on bottom bracket 26, and bottom bracket 26 is arranged on slide rail 21.
Using method of the present invention: controller 9 controls the second motor 11 and starts, makes the second gear 14 rotate, and drives the first gear 6 to rotate, support plate 5 can be made to rotate, PCR instrument main frame 29 is rotated, change PCR instrument main frame 29 towards; Spring 4 and elastic rod 3 can avoid PCR instrument main frame 29 to produce larger rocking when being knocked, and make PCR instrument main frame 29 preserve steadily; Controller 9 controls the first motor 27 and starts, and leading screw 23 is rotated, and screw 25 is moved up, and slide block 24 is moved up, and cover plate 30 is moved up, and test kit 1 is exposed; The energising of controller 9 control cylinder 20 internal solenoid valve or power-off, make the piston rod of cylinder 20 do stretching, makes test kit 1 outwards movement, avoids people manually to pull out test kit 1.
Claims (2)
1. a novel fluorescence quantitative PCR instrument, comprise test kit (1), upper plate (2), elastic rod (3), spring (4), support plate (5), first gear (6), roller bearing (7), flange bearing (8), controller (9), base plate (10), second motor (11), bracing frame (12), axle sleeve (13), second gear (14), second bearing pin (15), connecting rod (16), flake bearing (17), joint pin (18), first bearing pin (19), cylinder (20), slide rail (21), union lever (22), leading screw (23), slide block (24), screw (25), bottom bracket (26), first motor (27), support (28), PCR instrument main frame (29) and cover plate (30), it is characterized in that: the upper mounting flange bearing (8) of described base plate (10), flange bearing (8) is installed roller bearing (7), upper installation first gear (6) of roller bearing (7), under roller bearing (7) upper end is arranged on support plate (5), the second gear (14) each side installed by first gear (6), second gear (14) and the first gear (6) coincide mutually, second gear (14) is arranged on axle sleeve (13), axle sleeve (13) is arranged on the output shaft of the second motor (11), second motor (11) is arranged on base plate (10), bracing frame (12) is installed in axle sleeve (13) lower end, bracing frame (12) is arranged on base plate (10), controller (9) is arranged on base plate (10), controller (9) is connected with the second motor (11) by wire, support plate (5) is upper installs a more than spring (4) and a more than elastic rod (3), spring (4) and elastic rod (3) install upper plate (2), upper plate (2) is upper installs PCR instrument main frame (29), in PCR instrument main frame (29), test kit (1) is installed, test kit (1) each side installs flake bearing (17), flake bearing (17) is installed joint pin (18), the piston rod of cylinder (20) is arranged on joint pin (18) right side, cylinder (20) is arranged on PCR instrument main frame (29) side, test kit (1) each side installs the first bearing pin (19), connecting rod (16) is arranged on the first bearing pin (19), connecting rod (16) lower end is arranged on PCR instrument main frame (29) side by the second bearing pin (15), slide rail (21) each side installed by PCR instrument main frame (29), slide rail (21) is installed slide block (24), union lever (22) is installed in slide block (24) left side, between two union levers (22), cover plate (30) is installed, cover plate (30) covers test kit (1), screw (25) is installed on slide block (24) right side, screw (25) is arranged on leading screw (23), leading screw (23) upper end is connected with the first motor (27) by shaft coupling, first motor (27) is arranged on support (28), first motor (27) is connected with controller (9) by wire, support (28) is arranged on PCR instrument main frame (29), leading screw (23) two ends, left and right are respectively arranged on bottom bracket (26), bottom bracket (26) is arranged on slide rail (21).
2. a kind of novel fluorescence quantitative PCR instrument according to claim 1, it is characterized in that: described cylinder (20) internal solenoid valve, magnetic valve is connected with controller (9) by wire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510911542.9A CN105400680B (en) | 2015-12-11 | 2015-12-11 | A kind of quantitative real time PCR Instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510911542.9A CN105400680B (en) | 2015-12-11 | 2015-12-11 | A kind of quantitative real time PCR Instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105400680A true CN105400680A (en) | 2016-03-16 |
| CN105400680B CN105400680B (en) | 2017-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510911542.9A Active CN105400680B (en) | 2015-12-11 | 2015-12-11 | A kind of quantitative real time PCR Instrument |
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| Country | Link |
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| CN (1) | CN105400680B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105936869A (en) * | 2016-06-23 | 2016-09-14 | 浙江爱易生物医学科技有限公司 | Multifunctional fluorescent quantitative PCR instrument |
| CN105969638A (en) * | 2016-06-23 | 2016-09-28 | 浙江爱易生物医学科技有限公司 | Adjustable fluorogenic quantitative PCR instrument |
| CN106047683A (en) * | 2016-06-23 | 2016-10-26 | 浙江爱易生物医学科技有限公司 | Complete equipment for gene detection |
| CN106119075A (en) * | 2016-06-23 | 2016-11-16 | 浙江爱易生物医学科技有限公司 | A kind of real-time fluorescence quantitative PCR system |
| CN106318863A (en) * | 2016-10-14 | 2017-01-11 | 上海爱易生物医学科技股份有限公司 | System for gene detection |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105936869A (en) * | 2016-06-23 | 2016-09-14 | 浙江爱易生物医学科技有限公司 | Multifunctional fluorescent quantitative PCR instrument |
| CN105969638A (en) * | 2016-06-23 | 2016-09-28 | 浙江爱易生物医学科技有限公司 | Adjustable fluorogenic quantitative PCR instrument |
| CN106047683A (en) * | 2016-06-23 | 2016-10-26 | 浙江爱易生物医学科技有限公司 | Complete equipment for gene detection |
| CN106119075A (en) * | 2016-06-23 | 2016-11-16 | 浙江爱易生物医学科技有限公司 | A kind of real-time fluorescence quantitative PCR system |
| CN105936869B (en) * | 2016-06-23 | 2018-05-25 | 浙江爱易生物医学科技有限公司 | A kind of Multifunction fluorescent quantitative PCR apparatus |
| CN106119075B (en) * | 2016-06-23 | 2018-09-04 | 浙江爱易生物医学科技有限公司 | A kind of real-time fluorescence quantitative PCR system |
| CN105969638B (en) * | 2016-06-23 | 2018-11-30 | 浙江爱易生物医学科技有限公司 | A kind of adjustable fluorescence quantitative PCR instrument |
| CN106318863A (en) * | 2016-10-14 | 2017-01-11 | 上海爱易生物医学科技股份有限公司 | System for gene detection |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105400680B (en) | 2017-12-15 |
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Effective date of registration: 20220414 Address after: 102600 Room 403, unit 1, 15th floor, changfengyuan, Huangcun Town, Daxing District, Beijing Patentee after: Du Lihong Address before: 316021 No. 739, dingshen Road, Lincheng street, Dinghai District, Zhoushan City, Zhejiang Province Patentee before: ZHOUSHAN Hospital |
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Effective date of registration: 20220711 Address after: 300000 Tianjin International Biomedical Joint Research Institute n1012, No. 220 Dongting Road, economic and Technological Development Zone, Binhai New Area, Tianjin Patentee after: TIANJIN MOGENETICS BIOTECHNOLOGY CO.,LTD. Address before: 102600 Room 403, unit 1, 15th floor, changfengyuan, Huangcun Town, Daxing District, Beijing Patentee before: Du Lihong |
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Effective date of registration: 20220824 Address after: Room D209, 2nd Floor, Building 1, No. 12, Shangdi Information Road, Haidian District, Beijing 100000 Patentee after: BEIJING FIRE GENE DX TECHNOLOGY CO.,LTD. Address before: 300000 Tianjin International Biomedical Joint Research Institute n1012, No. 220 Dongting Road, economic and Technological Development Zone, Binhai New Area, Tianjin Patentee before: TIANJIN MOGENETICS BIOTECHNOLOGY CO.,LTD. |
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