CN105325282A - Oil-used paeonia lactiflora first-generation hybrid seed production method and seed uses - Google Patents
Oil-used paeonia lactiflora first-generation hybrid seed production method and seed uses Download PDFInfo
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- CN105325282A CN105325282A CN201510625973.9A CN201510625973A CN105325282A CN 105325282 A CN105325282 A CN 105325282A CN 201510625973 A CN201510625973 A CN 201510625973A CN 105325282 A CN105325282 A CN 105325282A
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- herbaceous peony
- chinese herbaceous
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 30
- 244000236658 Paeonia lactiflora Species 0.000 title abstract description 9
- 235000008598 Paeonia lactiflora Nutrition 0.000 title abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 49
- 238000002955 isolation Methods 0.000 claims abstract description 16
- 239000008157 edible vegetable oil Substances 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 241000736199 Paeonia Species 0.000 claims description 126
- 235000006484 Paeonia officinalis Nutrition 0.000 claims description 124
- 241000196324 Embryophyta Species 0.000 claims description 41
- 230000008774 maternal effect Effects 0.000 claims description 36
- 239000003921 oil Substances 0.000 claims description 33
- 235000019198 oils Nutrition 0.000 claims description 33
- 230000010152 pollination Effects 0.000 claims description 19
- 201000010099 disease Diseases 0.000 claims description 17
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 17
- 239000007921 spray Substances 0.000 claims description 13
- 230000008775 paternal effect Effects 0.000 claims description 10
- 238000010899 nucleation Methods 0.000 claims description 8
- 239000005980 Gibberellic acid Substances 0.000 claims description 7
- 239000005983 Maleic hydrazide Substances 0.000 claims description 7
- BGRDGMRNKXEXQD-UHFFFAOYSA-N Maleic hydrazide Chemical compound OC1=CC=C(O)N=N1 BGRDGMRNKXEXQD-UHFFFAOYSA-N 0.000 claims description 7
- 230000005200 bud stage Effects 0.000 claims description 7
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 7
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 6
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- 230000002269 spontaneous effect Effects 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 abstract description 5
- 208000035240 Disease Resistance Diseases 0.000 abstract 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
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- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 5
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 5
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 5
- 239000005642 Oleic acid Substances 0.000 description 5
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 5
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 5
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 5
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 4
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 235000014113 dietary fatty acids Nutrition 0.000 description 4
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- YKRGDOXKVOZESV-UHFFFAOYSA-N paeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(CO)O5)O)CC3(O)OC1C24COC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-UHFFFAOYSA-N 0.000 description 4
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 3
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 229960004488 linolenic acid Drugs 0.000 description 3
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 3
- 230000008569 process Effects 0.000 description 3
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- UILPJVPSNHJFIK-UHFFFAOYSA-N Paeonol Chemical compound COC1=CC=C(C(C)=O)C(O)=C1 UILPJVPSNHJFIK-UHFFFAOYSA-N 0.000 description 2
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- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
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- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
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- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- STECJAGHUSJQJN-UHFFFAOYSA-N N-Methyl-scopolamin Natural products C1C(C2C3O2)N(C)C3CC1OC(=O)C(CO)C1=CC=CC=C1 STECJAGHUSJQJN-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000123968 Paeonia anomala Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
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- 235000019784 crude fat Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- YLTGFGDODHXMFB-UHFFFAOYSA-N isoacetovanillon Natural products COC1=CC=C(C(C)=O)C=C1O YLTGFGDODHXMFB-UHFFFAOYSA-N 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
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- 230000013011 mating Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 235000021313 oleic acid Nutrition 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- MLIBGOFSXXWRIY-UHFFFAOYSA-N paeonol Natural products COC1=CC=C(O)C(C(C)=O)=C1 MLIBGOFSXXWRIY-UHFFFAOYSA-N 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
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- STECJAGHUSJQJN-FWXGHANASA-N scopolamine Chemical compound C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-FWXGHANASA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses an oil-used paeonia lactiflora first-generation hybrid seed production method and seed uses. The method includes the following steps: choosing any one or any two or three good-character male sterile lines having high quality, high yield and disease resistance from seed buds of Bozhou paeonia lactiflora or Sichuan paeonia lactiflora to be used as a female parent; (2) choosing a male parent, and selecting any one or any two or three good-character selfing lines under an isolation state and with high quality, high yield and disease resistance from seedlings of seed setting type paeonia lactiflora to be used as the male parent; (3) selecting an isolation zone; (4) transplanting and carrying out fixed planting; (5) adjusting a flowering period; (6) pollinating; and (7) harvesting. The use of seeds harvested from the female parent by the seed production method comprises that seeds are directly used in field planting production; and the uses of seeds harvested from the male parent comprise that the seeds are used as raw material for production of edible oil and used as a male parent for production of next-generation seeds. The production method has the beneficial effects that the first-generation hybrid is firstly produced; seed production procedures are simplified, and the production cost is reduced; and demand of mass production of the high-quality paeonia lactiflora and seed grains thereof and manufacture of edible vegetable oil can be met.
Description
Technical field
The invention belongs to field of plant breeding, be specifically related to the novelty teabag of a kind of oil Chinese herbaceous peony first generation cross-fertilize seed producing method for seed and seed.
Background technology
Chinese herbaceous peony, for hair section Paeonia perennial herb medicinal plant Chinese herbaceous peony, root hyoscine, its main component Chinese herbaceous peony addicted to, paeonol etc., bitter, to be slightly cold, returning Liver Channel, is the conventional key medicine of China traditional Chinese medicine tradition, medicinal herb grower's plantation of 80 percent be all based on the farm variety of Bo medicine, river medicine, and Bo medicine and river medicine are mostly by vegetative propagation, before will waiting until, season Chinese herbaceous peony growth separately uses bud head after gathering in the crops for many years as seedling, and do not produce seed, breeding radix is low, cycle is long, is unfavorable for large area Industry Promotion and mechanization production.
At present, after the present inventor utilizes wild RADIX PAEONIAE RUBRA and family to plant paenoiae alba hybridization, isolated heterozygosis seed does sexual propagation, and this seed production is relatively low.Show according to professional institution's research: crude fat more than 20% contained by Chinese herbaceous peony seed, oleic acid, linoleic acid, linolenic acid ratio is contained all between 25%-35% in its grease, three kinds of unsaturated fatty acid ratios are close to balanced, its linolenic content is 50 times of homogenous quantities olive oil on the market, be at present known to one of best in the world green health edible oil resource.
Modern nutriology is thought: the oleic acid in plant in unsaturated fatty acid has the effect regulating high and low density cholesterol concentration ratio in blood of human body; Linoleic acid has the effect reducing cholesterol and blood fat in blood of human body; Linolenic acid have remove human free radical develop immunitypty, reduction and stabilizing blood pressure and blood sugar, increase intelligence, vision protection and antioxidation; high nutraceutical development and utilization is had to be worth; the possible threat that the edible oils such as current genetically engineered soybean, cotton, corn are particularly formed health of people; the trend gone to the world along with the great rejuvenation of the Chinese nation and traditional Chinese medicine and trend, Chinese herbaceous peony seed edible oil has more irreplaceable strategic value and economic worth.
The above-mentioned method producing a small amount of Chinese herbaceous peony seed, be called conventional breeding method (being also systematic breeding method), but, still there are some problems in this producing method for seed, be mainly manifested in: though the seed of plant results, but yield poorly, and these seeds belong to the allometrosis of extremely unstable, offspring is separated seriously, production traits extremely unstable, plant property poor, as: Chinese herbaceous peony plant height is uneven, plant is general thinner and more delicate, extent of the root system, diameter is all undesirable, resistance against diseases is poor, seed size is inconsistent, ripe sooner or later also inconsistent, angle fruit (seed) is easily sagging, be unfavorable for mechanized harvest and establishing in large scale, and this method will through seed selection, 4-7 is for selfing supervisor, cycle is long, program is loaded down with trivial details, manually pay cost high.
Therefore, conventional breeding method (being also systematic breeding method) seriously governs the commercial development of Chinese herbaceous peony edible oil because of all drawbacks such as production of hybrid seeds long flow path, seed properties root of herbaceous peony quality that is unstable, that bring out seed regenerative ratio are not ideal, also affects the performance of some merits of Chinese medicine Chinese herbaceous peony.
Summary of the invention
Object of the present invention provides a kind of oil Chinese herbaceous peony producing method for seed and seed purposes, the method utilizes three series mating Theory and technology, to be set seeds on paternal plant grain by this method, maternal plant bears seeds, and the seed that maternal plant is born is the Chinese herbaceous peony first generation cross-fertilize seed (F1) possessing the excellent proterties of parents, field seeding can be directly used in produce, production of hybrid seeds flow process is short, F1 generation proterties is stablized, well, and the seed of future generation sowed by F1 generation seed that the grain yield of future generation produced produces compared with conventional breeding method and produce exceeds 30-50%, F1 generation Paeonia lactiflora seed meets commercialization, the needs of large-scale production high-quality Chinese herbaceous peony and Chinese herbaceous peony seed, its Chinese herbaceous peony seed can meet manufacture high-quality, the demand of high-grade vegetable edible oil.
For realizing goal of the invention, the technical scheme of employing is:
1, oil Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, is characterized in that: comprise the steps:
(1) female parent is selected
Select to have from Bo Chinese herbaceous peony or river Chinese herbaceous peony kind bud high-quality, high yield, disease-resistant any one or any two or three kinds of good proterties male sterile line do maternal;
(2) male parent is selected
Select to have from the seedling of class Chinese herbaceous peony of setting seeds high-quality, high yield, disease-resistant any one or any two or three kinds of good proterties and be that inbred line under isolation situation makes male parent;
(3) isolated area is selected
The regional arrangement hybrid seeding field of the class Chinese herbaceous peony that do not blossom and have seeds in 5-10 kilometer around plantation, to get rid of the interference of assorted system;
(4) transplant planting
To the maternal budding described in step (1), male parent seedling, the female parent kind bud that divided are transplanted to put in order by the large Tanaka of the isolated area interval field planting field in proportion described in step (3);
(5) florescence alternation, promotes male parent, maternal florescence consistent;
(6) pollinate: female parent is pollinated;
(7) gather: when seed maturity, to gather seed from paternal plant, to gather seed (F1) from maternal plant.
For better realizing the pollination to female parent, pollination is any one or the combination of wantonly two kinds or the combination of three kinds in spontaneous pollination, supplementary pollination, artificial pollination.
For the florescence of promotion male parent, maternal plant is consistent, for the male parent having Blooming to be inclined to or female parent in step (5), can bloom to postpone it with the directed foliage spray of the maleic hydrazide of 100ppm at bud stage, spray 1-3 time, the consumption of every mu of maleic hydrazide aqueous solution is 20-60 kilogram, promotes male parent, maternal florescence consistent.
For the florescence of promotion male parent, maternal plant is consistent, for having the male parent or female parent of postponing tendency of blooming in step (5), can at the directed foliar spray of the gibberellic acid of bud stage 40ppm to impel its Blooming, spray 1-3 time, every mu of gibberellic acid amount of aqueous solution used is 20-60 kilogram, promotes male parent, maternal florescence consistent.
For convenience of the seed of father, maternal results, seed later maintenance and application, comprise step (8): the seed born of the seed born male parent, female parent, separately after results, carries out airing respectively, collects and manage and protect.
The first purposes of product is directly obtained: a kind of seed adopting oil according to claim 1 to gather from maternal plant with Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, this seed is first generation cross-fertilize seed (F1), produces for field seeding as the inventive method.
Directly obtain the second purposes of product as the inventive method: a kind of seed adopting oil according to claim 1 to gather from paternal plant with Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, the purposes of this seed is the raw material as producing edible vegetable oil.
The third purposes of product is directly obtained: a kind of seed adopting oil according to claim 1 to gather from paternal plant with Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, the purposes of this seed is the male parent as claim 1 step (2) described production seed of future generation as the inventive method.
Good effect of the present invention is mainly manifested in:
1, pioneering first generation cross-fertilize seed.
Adopt this method, cultivate pioneering Chinese herbaceous peony first generation cross-fertilize seed (F1) both at home and abroad, first generation cross-fertilize seed proterties is stablized, possesses parents' advantageous feature (showing plant characteristic etc.), the special seed of Production of Large Fields can be made, overcome that conventional breeding legal system obtains that Paeonia lactiflora seed obtains for Production of Large Fields that Chinese herbaceous peony yields poorly, poor quality and seed is unstable, easily separated, plant sexual involution problem.
2, simplify production of hybrid seeds program, reduce seed produces cost.
Conventional breeding method first hybridizes the sexual method of breeding be separated again, and finally just will can select through repeatedly seed selection, 4-7 the seed meeting breeding objective for selfing, program is loaded down with trivial details, and breeding time generally takes 5-10 years; And the inventive method simple, be easy to grasp, easy to utilize, breeding time is short, within general about 2 years, just can set seeds, high yield after 3-5, and utilization periods can reach more than 10 years, and reproductive efficiency is high, is easy to the large-scale production production of hybrid seeds and results.
3, the raw material needs meet the industrialization of Chinese herbaceous peony oil, commercially producing.
The Paeonia lactiflora seed adopting the present invention to produce and seed, is applicable to implant mass and breeds production again, and every oil-containing nutrient component index is not less than the seed of conventional breeding method, can meet industrialization, commercially produce the needs of the raw material of Chinese herbaceous peony oil.
4, Social and economic benef@is considerable.
Adopt this producing method for seed, meet the needs of Chinese herbaceous peony oil raw material in traditional Chinese medicine, health products and food on the one hand, for the health of the mankind and longevity contribute, on the other hand, improve mechanized cultivation, the implant mass degree of Chinese medicine Chinese herbaceous peony and Chinese herbaceous peony oil raw material, alleviate manual labor's burden, greatly improve the income at vast plantation family, therefore there is good economic benefit and social benefit.
Accompanying drawing explanation
Fig. 1 is male parent seedling of the present invention, female parent plants one of field ratio schematic diagram of bud;
Fig. 2 is male parent seedling of the present invention, maternal field ratio schematic diagram two of planting bud.
Embodiment
By following some embodiments, describe the present invention in detail.
embodiment 1
(1) female parent is selected
From the Chinese herbaceous peony kind bud of river, select the male sterile line with good proterties to do maternal to plant bud: refer to through selecting, select there are any one or any two combinations in high-quality, high yield, these three kinds of characteristics disease-resistant or three kinds of good proterties entirely possessed and the kind bud of river Chinese herbaceous peony for male sterile line.The kind of good proterties is how many, fixed according to breeding objective, than if any breeding objective focus on output (rhizome, plant grain output), the male sterile line possessing the good proterties of " high yield " when focal selection does female parent, some breeding objectives are the characteristics focusing on " high yield " and " disease-resistant ", the male sterile line simultaneously possessing " high yield " and " disease-resistant " two kinds of superperformances when focal selection does female parent, how breeding objective is selected, how good proterties kind is determined, all belong to prior art, one skilled in the art all can realize, and no longer describe in detail here.
The system of selection of good proterties river Chinese herbaceous peony kind bud is conventional method, and as long-term observation, comparative trial, analytic statistics etc., selecting party prior art owned by France, one of ordinary skill in the art all can realize, and no longer describe in detail here.
Maternal embodiment of planting bud is the agricultural methods of existing routine, such as takes bud head to breed: to cut several piece, stay bud, sprout tuber is buried soil pit cultivation etc., no longer describe in detail here.
(2) male parent is selected
From class Chinese herbaceous peony of setting seeds---select wild RADIX PAEONIAE RUBRA seedling to have good proterties and be that under isolation, inbred line makes male parent seedling: referring to through selecting, there are in selecting any one or any two combination in high-quality, high yield, these three kinds of characteristics disease-resistant or three kinds of good proterties entirely possessed and be the seedling of the wild RADIX PAEONIAE RUBRA of the inbred line under isolation.The kind of good proterties is how many, fixed according to breeding objective, than if any breeding objective focus on output (rhizome, plant grain output), the inbred line possessing " high yield " good proterties when focal selection makes male parent, some breeding objectives are the characteristics focusing on " high yield " and " disease-resistant ", the inbred line seedling simultaneously possessing " high yield " and " disease-resistant " two kinds of superperformances when focal selection makes male parent, how breeding objective is selected, how good proterties kind is determined, belong to prior art, one skilled in the art all can realize, and no longer describe in detail here.
The system of selection of the wild RADIX PAEONIAE RUBRA seedling of good proterties is conventional method, and as long-term observation, measurement, contrast, test, analytic statistics etc., selecting party prior art owned by France, one skilled in the art all can realize, and no longer describe in detail here.
This wild RADIX PAEONIAE RUBRA inbred line is generally selfing 3 generation more than, preferably 3 generations selfing, and selfing realizes under isolation, isolation comprises the partition methods such as space isolation, artificial gauze isolation, belong to prior art, one skilled in the art all can realize, and no longer describe in detail here.
The embodiment of male parent seedling is existing conventional agriculture method, such as conventional seed seedling-raising: comprise soil sowing, germinating growth is root of herbaceous peony seedling etc.Here no longer describe in detail.
(3) isolated area is selected
The regional arrangement hybrid seeding field of the class Chinese herbaceous peony that do not blossom and have seeds in 5-10 kilometer around plantation, to get rid of the interference of assorted system.
Class of blossoming and having seeds Chinese herbaceous peony mainly comprises wild class Chinese herbaceous peony of setting seeds, Hangzhoupro Chinese herbaceous peony, artificial culture " No. one, oily Chinese herbaceous peony ", wherein, wild class Chinese herbaceous peony of setting seeds mainly comprises: wild RADIX PAEONIAE RUBRA, root of Paeonia sinjiangensis, mutation Peony, wildly spend more the kind such as Chinese herbaceous peony, wild single flower Chinese herbaceous peony.
(4) transplant planting
In annual autumn (August-November during), to the maternal budding described in step (1), male parent seedling, the female parent kind bud that divided are transplanted to put in order by the large Tanaka of the isolated area interval field planting field in proportion described in step (3).
In this step, indication " in proportion " interval field planting belongs to existing routine techniques, object is convenient to pollination and results seed, there is no fixing proportional manner, interval mode, one of ordinary skill in the art know, according to pollination scope, uniformity coefficient and whether take artificial pollination, and how to facilitate artificial pollination, how to facilitate results seed, selection percentage, interval mode flexibly voluntarily.Such as: the present invention is preferably: as shown in Figure 1, field margins place ratio is: left hand edge a line is maternal row, right hand edge adjacent male parent row: maternal behavior 1:1, field mid portion: male parent row and the capable ratio of female parent are 1:2, also can be as shown in Figure 2, field mid portion: male parent row and the capable ratio of female parent are 1:3, also can be other proportional spacing pattern, no longer describe in detail here.
(5) florescence alternation, promotes male parent, maternal florescence consistent.
The male parent be inclined to for there being Blooming, maternal can with the directed foliage spray of the maleic hydrazide of 100ppm with late blooming at bud stage, sprays 1-3 time, the consumption of every mu of maleic hydrazide aqueous solution is 20-60 kilogram, and promotion male parent, female parent florescence are consistent;
For have postpone tendency of blooming male parent, female parent can at the directed foliar spray of the gibberellic acid of bud stage 40ppm to impel Blooming, sprays 1-3 time, the consumption of every mu of gibberellic acid aqueous solution is 20-60 kilogram, and promotion male parent, female parent florescence are consistent.
Maleic hydrazide, gibberellic acid specifically spray number of times and every mu of consumption, how many according to the time that male parent, maternal bud stage stagger, and grasp flexibly voluntarily.
(6) pollinate: female parent is pollinated.
For better realizing the pollination to female parent, pollination is any one or the combination of wantonly two kinds or the combination of three kinds in spontaneous pollination, supplementary pollination, artificial pollination.
Supplementary pollination mainly adopts wild bee or manually puts honeybee in a suitable place to breed to realize.
(7) gather: when seed maturity, to gather seed (F1) from gather seed, maternal plant of paternal plant.
The seed that seed that paternal plant is gathered, maternal plant are born separately results during results, be applied to different purposes, the seed of the seed also paternal plant can gathered in the crops, maternal plant results mixes, all for the manufacture of edible oil.
(8) divide solarization, point to hide, be in charge of: during results the seed airing respectively of the seed of male parent results, maternal results, collect and manage and protect, produce and application as the later stage.
Also according to situation on the spot, selectively can comprise: the step such as whole ground basal dressing, root outer spraying, the extermination of disease and insect pest, intertill and clean tillage, field management, all belongs to conventional prior art, no longer describe in detail here in said process.
embodiment 2
(1) female parent is selected
From Bo Chinese herbaceous peony kind bud, select the male sterile line with good proterties to do maternal to plant bud: refer to through selecting, select there are any one or any two combinations in high-quality, high yield, these three kinds of characteristics disease-resistant or three kinds of good proterties entirely possessed and be the Bo Chinese herbaceous peony kind bud of male sterile line.How good proterties kind is determined, the kind bud selection selection method of good proterties Bo Chinese herbaceous peony is identical with " river Chinese herbaceous peony " in embodiment 1 step (1), and one skilled in the art all can realize, and no longer describe in detail here.
The embodiment of Bo Chinese herbaceous peony kind bud is the agricultural methods of existing routine, identical with the embodiment that " river Chinese herbaceous peony " in embodiment 1 step (1) plants bud, no longer describes in detail here.
(2) male parent is selected
From class Chinese herbaceous peony of setting seeds---select the Chinese herbaceous peony seedling of Hangzhoupro to have good proterties and be that under isolation, inbred line makes male parent seedling: refer to through selecting, select there are any one or any two combination in high-quality, high yield, these three kinds of characteristics disease-resistant or three kinds of proterties entirely possessed and be the seedling of the Hangzhoupro Chinese herbaceous peony of the inbred line under isolation.How good proterties kind is determined, the Hangzhoupro Chinese herbaceous peony seedling system of selection of good proterties is identical with " the wild RADIX PAEONIAE RUBRA " in embodiment 1 step (2), and one skilled in the art all can realize, and no longer describe in detail here.
Hangzhoupro Chinese herbaceous peony inbred line, isolated instances are identical with " the wild RADIX PAEONIAE RUBRA " in embodiment 1 step (2), and one skilled in the art all can realize, and no longer describe in detail here.
The embodiment of Hangzhoupro Chinese herbaceous peony seedling is existing conventional agriculture method, identical with " wild RADIX PAEONIAE RUBRA " type of rearing in embodiment 1 step (2), no longer describes in detail here.
Step (3)-(8) are corresponding identical with step (3)-(8) in embodiment 1, therefore omit.
embodiment 3
First, introduce a kind of novel class Chinese herbaceous peony of setting seeds---" No. one, oily Chinese herbaceous peony ", " No. one, oily Chinese herbaceous peony " (code name, lower same) be a kind of Chinese herbaceous peony new varieties, it is many for selection cross (i.e. aforesaid conventional breeding method) to be that the present inventor's root of herbaceous peony variety source Hangzhoupro Chinese herbaceous peony farm variety of utilizing China famous and wild herbaceous peony carry out, this kind generates a small amount of seed, every fruit is on average set seeds grain about 30, this product plant height 1-1.5 rice, blade is more roomy, blade face is thinner, color is more shallow, seed oil content is more than 20%, the general per mu yield of life in 4 years of this kind medicinal effects (rhizome) can reach more than 1500 kilograms, one, secondary product rate is high, than farm variety volume increase 15%-20%(when not affecting seed produces), seed is full rear easily sagging, be unfavorable for mechanized harvest, paeoniflorin content exceeds more than 20% than farm variety.
(1) female parent is selected
In the present embodiment, step (1) " seed selection is maternal " is identical with step (1) " seed selection the is maternal " content in embodiment 1, therefore omits.Female parent selection object is " river Chinese herbaceous peony " or " Bo Chinese herbaceous peony ".
(2) male parent is selected
From class Chinese herbaceous peony of setting seeds---select to have good proterties " No. one, oily Chinese herbaceous peony " seedling, and be that inbred line under isolation makes male parent seedling: refer to through selecting, select and there is high-quality, high yield, any one or any two combination in these three kinds of characteristics disease-resistant or three kinds of good proterties entirely possessed, and be the seedling of inbred line under isolation " No. one, oily Chinese herbaceous peony ", how the kind of good proterties is determined, " No. one, oily Chinese herbaceous peony " seedling system of selection of good proterties is identical with " the wild RADIX PAEONIAE RUBRA " of step (2) in embodiment 1, one skilled in the art all can realize, here no longer describe in detail.
" No. one, oily Chinese herbaceous peony " inbred line, isolated instances are identical with " the wild RADIX PAEONIAE RUBRA " in embodiment 1 step (2), and one skilled in the art all can realize, and no longer describe in detail here.
" No. one, oily Chinese herbaceous peony " seedling fostering mode is existing conventional agriculture method, identical with " wild RADIX PAEONIAE RUBRA " type of rearing in embodiment 1 step (2), no longer describes in detail here.
The present embodiment step (3)-(8) are corresponding identical with step (3)-(8) in embodiment 1, therefore omit.
embodiment 4
In the present embodiment, content is identical " to select female parent " with the step (1) in embodiment 1 for step (1) " selection is maternal ".Therefore omit.
(2) male parent is selected
From class Chinese herbaceous peony of setting seeds---select the inbred line with good proterties to make male parent seedling " mutation Peony " seedling: refer to through selecting, select and there is high-quality, high yield, any one or any two combination in these three kinds of characteristics disease-resistant or three kinds of proterties entirely possessed, and be the seedling of the mutation Peony of the inbred line under isolation, how good proterties kind is determined, " mutation Peony " seedling system of selection of good proterties is identical with " the wild RADIX PAEONIAE RUBRA " in embodiment 1 step (2), one skilled in the art all can realize, here no longer describe in detail.
" mutation Peony " inbred line, isolated instances are identical with " the wild RADIX PAEONIAE RUBRA " in embodiment 1 step (2), and one skilled in the art all can realize, and no longer describe in detail here.
" mutation Peony " seedling embodiment is existing conventional agriculture method, and to cultivate embodiment identical with " wild RADIX PAEONIAE RUBRA " in step (2) in embodiment 1, no longer describes in detail here.
The present embodiment step (3)-(8) are corresponding identical with step (3)-(8) in embodiment 1, therefore omit.
embodiment 5
In the present embodiment, content is identical " to select female parent " with the step (1) in embodiment 1 for step (1) " selection is maternal ".Therefore omit.
(2) male parent is selected
From class Chinese herbaceous peony of setting seeds---select the inbred line with good proterties to make male parent seedling " root of Paeonia sinjiangensis ": refer to through selecting, select and there are any one or any two combinations in high-quality, high yield, disease-resistant these three kinds of characteristics strong or three kinds of proterties entirely possessed and be the seedling of the root of Paeonia sinjiangensis of the inbred line under isolation, how good proterties kind is determined, the system of selection of " root of Paeonia sinjiangensis " seedling of good proterties is identical with " the wild RADIX PAEONIAE RUBRA " of embodiment 1 step (2), one skilled in the art all can realize, and no longer describe in detail here.
" root of Paeonia sinjiangensis " inbred line, isolated instances are identical with " the wild RADIX PAEONIAE RUBRA " in embodiment 1 step (2), and one skilled in the art all can realize, and no longer describe in detail here.
" root of Paeonia sinjiangensis " seedling embodiment is existing conventional agriculture method, identical with " wild RADIX PAEONIAE RUBRA " type of rearing in step (2) in embodiment 1, no longer describes in detail here.
The present embodiment step (3)-(8) are corresponding identical with step (3)-(8) in embodiment 1, therefore omit.
In addition, the Chinese herbaceous peony that can be used as male parent seedling described in the inventive method step (2) also comprises: wildly spend more Chinese herbaceous peony, the red spoon of mutation hair, beautiful Chinese herbaceous peony, careless Chinese herbaceous peony and mutation Mao Yecao Chinese herbaceous peony thereof, spend Chinese herbaceous peony, Paeonia anomala etc. in vain and to set seeds class Chinese herbaceous peony.
Apart from the above, the seed utilizing the inventive method to gather in the crops in male parent can be used as male parent when producing seed of future generation, can refer to step (2), carrys out choice for use.
Below by some tests, detect, good effect of the present invention is described.
test one:
Inventor is through great many of experiments, the present invention is adopted to have many advantages than conventional breeding method, such as: in October, 2011 plants, check and accept on August 10th, 2014, " " (adopt the first generation cross-fertilize seed F1 that producing method for seed of the present invention obtains at female parent, the present inventor is by this Hybrid F1 called after " assorted No. one of oil " for assorted No. one of oil for the present inventor's No. 1 in Sha Ji township of Yucheng County of Henan Province farmland covered by contract plantation, one mu down together), plantation " No. one, oily Chinese herbaceous peony " (male parent) a mu, contrast by analysis, conclusion is as following table:
| the production of hybrid seeds cycle | whether mechanization | production of hybrid seeds step | seed outward appearance | whether seeding comprises first generation cross-fertilize seed | grain yield (2014) (pound/acre) | |
| the inventive method | gather in the crops seed after 2 years, annual results once. | fruit is put on an arrow at angle, is very convenient to mechanized harvest. | simply, convenient operation | full, in the same size | (female parent is gathered in the crops) | 235 |
| conventional breeding method | gather in the crops seed after 3 years, annual results once. | fruit is lower big-eared at angle, can not mechanical harvest, can only manually gather in the crops. | loaded down with trivial details, through too much generation is separated, unfavorable operation | having flatly has full, not of uniform sizely causes | no | 158 |
test two:
On August 1st, 2014 obtains result of the test: inventor congratulates certain farmland covered by contract six mu in Sha Ji West Street, Sha Ji township of Yucheng County of Henan Province, wherein plant " assorted No. one of oil " three mu, life in 3 years " assorted No. one of oil " average yield per mu seed 310 jin, plantation " No. one, oily Chinese herbaceous peony " three mu, life in 3 years " No. one, oily Chinese herbaceous peony " 220 jin, average yield per mu seed, amount of increase in production reaches 40.9%.
test three:
On July 30th, 2015 obtains result of the test: inventor appoints certain farmland covered by contract in Sha Ji township of Yucheng County of Henan Province Ren Qiaocunhuang building villager group, plantation " assorted No. one of oil " one mu, 4 years raw per mu yield seeds 480 jin, plantation " No. one, oily Chinese herbaceous peony " conventional kind one mu, 290 jin, per mu yield seed after same 4 years, amount of increase in production reaches 65.5%.
test four:
On July 26th, 2015, inventor is in Sha Ji township of Yucheng County of Henan Province Ren Qiao village Lee farmland covered by contract, plant two mu of root of herbaceous peonys (being life in 4 years), one mu of root of herbaceous peony (code name: Chinese herbaceous peony A) is the first generation root of herbaceous peony that the first generation cross-fertilize seed (F1) (code name: assorted No. one of oil) obtained by the present invention is generated, one mu of root of herbaceous peony (code name: Chinese herbaceous peony B) is the root of herbaceous peony generated by conventional breeding method acquisition seed (code name: No. one, oily Chinese herbaceous peony), plant characteristic is between the two through measuring, detecting, and results of comparison sees the following form:
detect 1:
The first generation cross-fertilize seed (F1) (code name: assorted No. one of oil) obtained by producing method for seed of the present invention grows up to the Chinese herbaceous peony rhizome of results, and the present inventor delivers Anhui Province's Bozhou City traditional Chinese medicine on March 27th, 2014 and imports and exports inspection center, and after testing, testing result is as follows:
" Chinese Pharmacopoeia " specifies: in the root of herbaceous peony, paeoniflorin content is not less than 1.8%.The paeoniflorin content being produced Chinese herbaceous peony by F1 generation of the present invention is 3.3%, far exceeds the regulation of pharmacopeia, also apparently higher than " root of herbaceous peony B " paeoniflorin content 2.4% in " test four ".
More than test, it is short compared with the conventional breeding method time that testing result fully indicates the present invention, production of hybrid seeds flow process is few, reproductive efficiency is high, the most important thing is that this method creates the Chinese herbaceous peony first generation cross-fertilize seed (F1) (inventor is from its code name of name: assorted No. one of oil) possessing the excellent proterties of parents, field seeding can be directly used in produce, F1 generation proterties is stablized, well, and F1 generation sowing produce grain yield of future generation comparatively conventional breeding method exceed 30-50%, the quality of the root of herbaceous peony bred by F1 generation, output, the indexs such as the property of medicine are obviously better than the root of herbaceous peony that conventional breeding method brings out seed produces.
detect 2:
The root of herbaceous peony seed (dry product) of being gathered in male parent by the inventive method, inventor proposes fatty acid inspection request on October 15th, 2014 to agricultural product quality supervision and inspection center of the Ministry of Agriculture (Zhengzhou), check through this center, in survey report, the every fatty acid index of root of herbaceous peony seed (dry product) is as following table:
Can find out that its seed can be used as good edible oil raw material by this report.Oleic acid, linoleic acid, linolenic index are higher, and ratio is more balanced, nutritious, adapts to very much the raw material as pure natural, health food oil.
detect 3:
The vegetable oil that the root of herbaceous peony grain seed (dry product) of being gathered in male parent by the inventive method is squeezed, inventor proposes fatty acid inspection request on October 15th, 2014 to agricultural product quality supervision and inspection center of the Ministry of Agriculture (Zhengzhou), check through this center, in survey report, the every fatty acid index of root of herbaceous peony seed (dry product) is as following table:
Can find out that this root of herbaceous peony oil oleic acid, linoleic acid, linolenic index are higher by this report, and it is very balanced, closely 1:1:1, without other poisonous and harmful elements, more existing oil plant type seeds (as peanut, soybean, camellia oilseeds etc.) the oleic acid of oil, linoleic acid, the linolenic acid that squeeze also not reach or close to above-mentioned scientific and rational natural ratio 1:1:1, therefore, the Chinese herbaceous peony seed produced by the present invention is suitable as the raw material of pure natural, healthy non-polluted edible oil very much, can meet commercialization, refine oil needs on a large scale.
The figure of above-mentioned expression, explanation; be only several preferred embodiment of the present invention; not that protection scope of the present invention is limited; to those skilled in the art; under the prerequisite not departing from the present invention's spirit and protection domain; the present invention probably has changes and improvements, and these changes and improvements all should fall within the scope of claimed the claims in the present invention.
Claims (8)
1. oil Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, is characterized in that: comprise the steps:
(1) female parent is selected
Select to have from Bo Chinese herbaceous peony or river Chinese herbaceous peony kind bud high-quality, high yield, disease-resistant any one or any two or three kinds of good proterties male sterile line do maternal;
(2) male parent is selected
Select to have from class Chinese herbaceous peony seedling of setting seeds high-quality, high yield, disease-resistant any one or any two or three kinds of good proterties and be that inbred line under isolation situation makes male parent;
(3) isolated area is selected
The regional arrangement hybrid seeding field of the class Chinese herbaceous peony that do not blossom and have seeds in 5-10 kilometer around plantation, to get rid of the interference of assorted system;
(4) transplant planting
To the maternal budding described in step (1), male parent seedling, the female parent kind bud that divided are transplanted to put in order by the large Tanaka of the isolated area interval field planting field in proportion described in step (3);
(5) florescence alternation, promotes male parent, maternal florescence consistent;
(6) pollinate: female parent is pollinated;
(7) gather: when seed maturity, to gather seed from paternal plant, to gather seed (F1) from maternal plant.
2. oil Chinese herbaceous peony first generation cross-fertilize seed producing method for seed according to claim 1, is characterized in that: step (6) pollination is any one or the combination of wantonly two kinds or the combination of three kinds in spontaneous pollination, supplementary pollination, artificial pollination.
3. oil according to claim 1 Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, it is characterized in that: for the male parent having Blooming to be inclined to or female parent in step (5), can bloom to postpone it with the directed foliage spray of the maleic hydrazide of 100ppm at bud stage, spray 1-3 time, the consumption of every mu of maleic hydrazide aqueous solution is 20-60 kilogram, promotes male parent, maternal florescence consistent.
4. oil according to claim 1 Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, it is characterized in that: for having the male parent or female parent of postponing tendency of blooming in step (5), at the directed foliar spray of the gibberellic acid of bud stage 40ppm, to impel in advance, it is bloomed, spray 1-3 time, every mu of gibberellic acid amount of aqueous solution used is 20-60 kilogram, promotes male parent, maternal florescence consistent.
5. oil Chinese herbaceous peony first generation cross-fertilize seed producing method for seed according to claim 1, is characterized in that: comprise step (8): the seed born of the seed born male parent, female parent, separately after results, carries out airing respectively, collects and manage and protect.
6. the seed adopting oil according to claim 1 to gather from maternal plant with Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, is characterized in that: this seed is first generation cross-fertilize seed (F1), produces for field seeding.
7. the seed adopting oil according to claim 1 to gather from paternal plant with Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, is characterized in that: the purposes of this seed is the raw material as producing edible vegetable oil.
8. the seed adopting oil according to claim 1 to gather from paternal plant with Chinese herbaceous peony first generation cross-fertilize seed producing method for seed, is characterized in that: the purposes of this seed is the male parent as claim 1 step (2) described production seed of future generation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109601372A (en) * | 2019-01-02 | 2019-04-12 | 四川鑫沃康农业有限责任公司 | A kind of breed improvement method of Radix Paeoniae Alba |
| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109601372A (en) * | 2019-01-02 | 2019-04-12 | 四川鑫沃康农业有限责任公司 | A kind of breed improvement method of Radix Paeoniae Alba |
| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
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