CN102715079A - Breeding method of novel variety of red-rooted salvia - Google Patents
Breeding method of novel variety of red-rooted salvia Download PDFInfo
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Abstract
The invention discloses a breeding method of a novel variety of red-rooted salvia. The breeding method of the novel variety of red-rooted salvia includes using a male sterile line SH-B-18 of red-rooted salvia as a female parent and a fertile line A-107 of red-rooted salvia as a male parent, and preparing the female parent and the male parent into a hybrid of red-rooted salvia. The breeding method of the novel variety of red-rooted salvia is applicable to novel high-yield, stable-yield, high-quality, disease-resistant and highly-applicable hybrid varieties of red-rooted salvia with fine comprehensive agronomic characters, which are planted in a base of Shangluo red-rooted salvia, even in same planting areas of China, and is applicable to the technical field of breeding.
Description
Technical field
The invention belongs to breeding technical field, relate to a kind of selection of red sage root new varieties.
Background technology
The root and the rhizome of the Labiatae herbaceous plant red sage root.Originate in ground such as Jiangsu, Anhui, Hebei, Sichuan.Spring, autumn excavate, and clean, and dry.Section, section, give birth to or wine fry and use.Bitter, cold nature.Can be promoting blood circulation and removing blood stasis, cool blood is calmed the nerves.Contain tanshinone, protocatechualdehyde, protocatechuic acid, danshensu, vitamin E etc.The ability coronary artery dilator increases coronary flow, improves myocardial ischemia, infraction and cardiac function, regulates the rhythm of the heart, and can expand peripheral vascular, improves microcirculation; Can improve body's hypoxia tolerance; Anticoagulation is arranged, promote fibrinolytic, suppress platelet aggregation, suppress thrombotic effect; Can reduce blood fat, suppress coronary atherosclerosis and form; Can suppress or alleviate degeneration of liver cells, necrosis and inflammatory reaction, promote liver cell regeneration, and anti-fibrosis effect is arranged; Can shorten the convalescence of red blood cell and hemochrome, Reticulocyte is increased, can promote the reparation of tissue, quicken the healing of fracture; There is nervous centralis that inhibitory action is arranged; Antitumor action is arranged; Ability enhancing body immunologic function; The ability blood sugar lowering; Various bacteria such as tubercle bacillus there is inhibitory action.
This shows that the red sage root is important Chinese herbal medicine commonly used, consumption is very big, is in great demand very much in medicinal material market.General its price comparison is stable, is one of comparatively ideal varieties of plant.But not smart as if shortage practical experience or technology in plantation, it is good results to occur, does not reach the income of expection.
Summary of the invention
In order to solve the problems of the technologies described above; The present invention provides a kind of selection of red sage root new varieties, and the high yield, stable yields, high-quality, the red sage root hybrid new breed disease-resistant, that adaptability strong, the comprehensive agronomy proterties is good that are fit to red sage root base, Shanglou and even the whole nation similar area plantation with seed selection are main purpose.Its technical scheme is following:
A kind of selection of red sage root new varieties, SH-B-18 does female parent with red sage root male sterile line, and fertile line red sage root A-107 does the formulated red sage root crossbreed of male parent.
The standard of maternal red sage root male sterile line SH-B-18 is following:
The biological property characteristic:
Phenophase: SH-B-18 strain system emerged at the beginning of 4 months by the end of March, mid-April bolting, late April buddings, the early flowering season at the beginning of 5 months, May the early and middle ten days full-bloom stage, seed maturity at the beginning of late June to 7 month, late October the medicinal material of gathering, mid or late November is withered.
Morphological feature: the SH-B-18 strain is leaf be ovum circle, gradually point; Leaf edge sawtooth shape; The blade face light green color has fold and utmost point down is arranged; The blade back light green color, fine hair is many and close, short; Stem is green, close tomentellate, corolla purple; Flower is sterile flower; Root is brick-red to red; The fruit sepia;
The overground part proterties: the SH-B-18 strain is plant height 30-55cm; The long 3.0-6.0cm of blade, wide 2.5.0-6.0cm; 3 go out or 5 go out, and minority 7 goes out compound leaf; Inflorescence main axis length 10-15cm; Branch amount 6-10; The thick 0.8-1.2cm of stem.
Kind attribute: this article kind male sterile red sage root.
Suitable area: suit in base, Shanglou and the whole nation similar area plantation.
The culture technique main points:
Seedling-cultivating method: dig out kind of a root before late October or soil are freeze-up, be cut into the 5-8cm segment, on hold level with both hands, the lower end is oblique.Ground is turned over, flattened, and 20 kilograms/mu of sealing fertilizers dig dark 20cm, the little furrow of wide 100-120cm, the long 10-12m of furrow; Make the bottom, seedbed loose smooth, in soil in the root segment straight cutting furrow, and keep the upper end upwards, lower ends downward, spacing in the rows 3-5cm; Line-spacing 5-10cm buries with wet soil and to cut root, and wet soil thickness and furrow face are closely flat, notes preventing freeze in winter, can be antifreeze with corn straw or film at the furrow face; After the beginning of spring, field soil thawed, scalp a furrow surface blinding, stay the 2-4cm blinding, prevent to damage the sprouting that sends.
Seedbed management: often keep the seedbed moistening, and in time pull out the seedbed weeds, cultivate strong sprout.
Cultivation method: can transplant when seedling grows 4 young leaves, in time water after planting.Every mu of high-quality farmyard manure 1000kg such as pig, cattle and sheep excrement that executes to become thoroughly decomposed makes base manure during transplanting, and seeding row spacing is 20cm * 25cm.
Field management: will in time weed with a hoe seedling stage, before the envelope ridge, topdresses 1-2 time, and envelope also will in time weed a garden behind the ridge.
Points for attention: (1) preceding crop is not selected legumes such as peanut, soybean.
Not continuous cropping when (2) planting is to avoid the generation of root knot nematode disease.
(3) with preceding stubble red sage root plantation interval 3-4.
Male parent fertile line red sage root A-107 standard is following:
The biological property characteristic:
Phenophase: at the beginning of 4 months, emerge at the bottom of the A-1073 month, mid-April bolting, late April buddings, the early flowering season at the beginning of 5 months, May the early and middle ten days full-bloom stage, seed maturity at the beginning of late June to 7 month, late October the medicinal material of gathering, mid or late November is withered.
Morphological feature: the A-107 strain is leaf be ovum circle, gradually point; Leaf edge sawtooth shape; The blade face light green color has fold and down is arranged; The blade back light green color, fine hair is many and close; Stem is green, close tomentellate, corolla purple; Flower is fertile flower; Root is red; The fruit sepia;
Kind attribute: this article kind male-fertile red sage root.
Suitable area: suit in base, Shanglou and the whole nation similar area plantation.
The culture technique main points:
Grow seedlings: when July, early and middle ten days was grown seedlings, ground is turned over, flattened, 20 kilograms/mu of sealing fertilizers earlier; Rake is thin, does the wide little furrow of wide 100-120cm, the drilling of ditching again; Ditch depth 2-4cm broadcasts not earthing of back, pats with broom or spade; Seed is contacted with soil closely, shelters from heat or light with wheat straw or shade net in ground again.
Seedbed management: often keep the seedbed moistening, in time throw off wheat straw or shade net after waiting to emerge, keep the Soil Dried wet alternate simultaneously, and in time pull out the seedbed weeds, cultivate strong sprout.
Cultivation method: treat that shoot root is long long and can transplant to 5-10cm.Every mu of high-quality farmyard manure 1000kg such as pig, cattle and sheep excrement that executes to become thoroughly decomposed makes base manure during transplanting, and seeding row spacing is 20cm * 25cm.
Field management: will in time weed with a hoe seedling stage, before the envelope ridge, topdresses 1-2 time, and envelope also will in time weed a garden behind the ridge.
Seed results:, should in time gather at this moment when fruit ear 2/3rds overstrikes are seed maturity.For guaranteeing seed quality, should gather immediately after the maturation, do not wait until that all fruit ear maturations gather again.
Points for attention: (1) preceding crop is not selected legumes such as peanut, soybean.
Not continuous cropping when (2) planting is to avoid the generation of root knot nematode disease.
(3) with preceding stubble red sage root plantation interval 3-4.
The crossbreed breeding:
It is convenient to select to irrigate, and soil fertility sandy loam on the upper side is as seed farm, and every mu of high-quality farmyard manure 1000kg such as pig, cattle and sheep excrement that executes to become thoroughly decomposed makes base manure; To turn over, flatten, rake is thin, trench digging; This capable of planting by 1: 2 or 2: 2 of father and mother, seeding row spacing is 20cm * 25cm.During plantation the seedling root all is embedded in the soil, will in time waters after planting, guarantee full stand.
The seed-breeding field management: will in time weed with a hoe seedling stage, before the envelope ridge, topdresses 1-2 time, and be main with fertilizer, N, P, K are used, and promote multi-branched and healthy and strong growth, guarantee full stand and healthy seedling.Envelope also will in time weed a garden behind the ridge.Florescence sprays potassium dihydrogen phosphate, to promote seed full.
The seed results: late June or early July,, fruit ear 2/3rds is seed maturity when becoming sepia, and can gather this moment, answers a ripe part results part, and receipts back airing is done back threshing in time on canvas or colour bar cloth.Should earlier the male parent acrial part be prescinded the seed of gathering in the crops again on the maternal plant before the results.
Strict production of hybrid seeds measure, carrying out anti-assorted protect pure: seed farm will with the red sage root of other kind or salvia spacing distance more than 1000m, seedling stage and each impurity elimination of florescence 1 time simultaneously, the strain of mixing of assorted fringe is rejected in close inspection during results.Seed grinds to be beaten, in the airing process, note preventing artificially and mechanical admixture.
Points for attention: preceding crop is not selected leguminous plants such as peanut, soybean; Not continuous cropping during plantation is to avoid the generation of root knot nematode disease; With preceding stubble red sage root plantation interval 3-4.
Compared with prior art, beneficial effect of the present invention:
Through combining ability test, A-107 and SH-B-18 strain cross offspring coordinate force are the highest, and resistance is best, and hybrid vigour is the most obvious.
The new varieties of seed selection of the present invention are had outstanding performance in comprehensive proterties such as output, resistance etc., and 968.84 kilograms of the bright medicines of average yield per mu are amounted to 261.20 kilograms of dried medicines.Compared test in continuous 3 years, and carry out kind zone comparative test at Shangzhou District, Shanyang County, Yangling District, the average raising the output 35.76% of contrast,
Characteristics such as new varieties of the present invention have high yield, stable yields, strong stress resistance, active constituent content height, and comprehensive proterties is good.Suit in base, Shanglou and even the good hybrid new breed of national similar regional popularizing planting.
Carry out variety comparative test at Shangzhou District, Shanyang County, Yangling District, 3 years 3 ground long run tests are average yield per mu 1032.97kg as a result, contrasts Shanglou red sage root average yield per mu 760.90kg raising the output 35.76%.
New varieties testing result of the present invention: tanshinone IIA content 0.56%; Content of danshinolic acid B 7.4%; Increase by 180% and 146.7% respectively than " pharmacopeia " regulation tanshinone IIA (0.2%), tanshin polyphenolic acid B (3.0%) content, comparison increases by 154.5% and 29.8% respectively according to kind Shanglou red sage root tanshinone IIA (0.22%) and tanshin polyphenolic acid B (5.7%).In district's examination, do not find root knot nematode disease, root rot, the fusarium wilt diseased plant is few, demonstrates the good characteristics of disease resistance.
Embodiment
Below in conjunction with specific embodiment method of the present invention is done explanation in further detail.
They red No. two is to do female parent with red sage root male sterile line SH-B-18, and fertile line red sage root A-107 does the formulated red sage root crossbreed of male parent.
The seed selection process:
The seed selection of male parent inbred line red sage root A-107: inbred line red sage root A-107 is that in May, 2003 is from the field production red sage root of Shanyang County, Shangluo City; The individual plant strain of selecting through systematic selection is; Its phenophase, resistance, coordinate force, quality and single plant yield are carried out network analysis, and at last according to overall target, wherein main content with danshensu, tanshinone and phenolic acid in the red rooted salvia is index; Confirm through combining ability test to be into roguing; Guarantee that each item index is stable, selecting at last into roguing is that A-107 is the male parent inbred line, and this inbred line is named red sage root A-107.
The seed selection of maternal " red sage root SH-B-18 male sterile line ": SH-B is that Xibei Univ. of Agricultural & Forest Science & Technology cooperates the strain male sterile plant that big Tanaka found in red sage root base in 2002 with sky, Shaanxi scholar's power plant pharmaceutcal corporation, Ltd; Through cutting root propagation, select growth and development stage and A-107 basically identical, sterility is thorough; Root bar number is more than 10; Root is scarlet, and the content of danshensu, tanshinone and phenolic acid is higher than local medicinal material and pharmacopeia regulation, the fine individual plant SH-B-18 that disease resistance is good; Cut root propagation continuously and select seed selection to form, name male sterile line into red sage root SH-B-18.
The hybridization assembly is through combining ability test, and A-107 and SH-B-18 strain cross offspring coordinate force are the highest, and resistance is best, and hybrid vigour is the most obvious, and its first generation of hybrid named is No. two, sky pellet.
Inbred line is produced:
Maternal male sterile line production: sterile line propagation adopts and cuts the root propagation method, digs out kind of a root before late October or soil are freeze-up, is cut into the 5-8cm segment, on hold level with both hands, the lower end is oblique.Ground is turned over, flattened, and 20 kilograms/mu of sealing fertilizers dig dark 20cm, the little furrow of wide 100-120cm, the long 10-12m of furrow; Make the bottom, seedbed loose smooth, in soil in the root segment straight cutting furrow, and keep the upper end upwards, lower ends downward, spacing in the rows 3-5cm; Line-spacing 5-10cm buries with wet soil and to cut root, and wet soil thickness and furrow face are closely flat, notes preventing freeze in winter, can be antifreeze with corn straw or film at the furrow face; After the beginning of spring, field soil thawed, scalp a furrow surface blinding, stay the 2-4cm blinding, prevent to damage the sprouting that sends.After the beginning of spring, often keep the seedbed moistening, and in time pull out the seedbed weeds, cultivate strong sprout.Can transplant when seedling grows 4 young leaves, in time water after planting.Every mu of high-quality farmyard manure 1000kg such as pig, cattle and sheep excrement that executes to become thoroughly decomposed makes base manure during transplanting, ridging behind the conventional whole ground, row spacing 100-120cm, furrow width 30cm, degree of depth 25cm; Every ridge plantation 5 row, spacing in the rows 20cm, every mu of total seedling is counted the 10000-12000 strain; Growing period is pressed each impurity elimination strain of male sterile line standard 2 times at seedling stage, florescence, guarantees male sterile line purity.When October, the kind root was gathered, remove undesirable kind of root, keep the good species root based on sterile line root standard.
The male parent fertile line is produced: ground turned over, flattened, and 20 kilograms/mu of sealing fertilizers, rake is thin; Do the wide little furrow of wide 1-1.2m, in annual July early and middle ten days grow seedlings the trench digging drilling; Ditch depth 2-3cm broadcasts not earthing of back, pats with broom, spade; Seed is contacted with soil closely, shelters from heat or light with wheat straw or shade net in ground again.Often keep the seedbed moistening, in time throw off wheat straw or shade net after waiting to emerge, keep the Soil Dried wet alternate simultaneously, and in time pull out the seedbed weeds, cultivate strong sprout.The long 5-10cm of shoot root, the soil preceding transplanting of freezing, or transplant the back that thaws the beginning of spring.Enhance field management vegetative period, mixes strain once by the male parent standard in seedling stage and each removal of florescence, to guarantee parental purity.When fruit ear 2/3rds overstrikes, promptly late June or early July will in time be gathered in the crops seed during seed maturity.Seed results, grind and beat, in the airing process, note preventing artificially and mechanical admixture.
The crossbreed breeding: it is convenient to select to irrigate, and soil fertility sandy loam on the upper side is as seed farm, and every mu of high-quality farmyard manure 1000kg such as pig, cattle and sheep excrement that executes to become thoroughly decomposed makes base manure; To turn over, flatten, rake is thin, trench digging; This capable of planting by 1: 2 or 2: 2 of father and mother, seeding row spacing is 20cm * 25cm.During plantation the seedling root all is embedded in the soil, will in time waters after planting, guarantee full stand.
The seed-breeding field management: will in time weed with a hoe seedling stage, before the envelope ridge, topdresses 1-2 time, and be main with fertilizer, N, P, K are used, and promote multi-branched and healthy and strong growth, guarantee full stand and healthy seedling.Envelope also will in time weed a garden behind the ridge.Florescence sprays potassium dihydrogen phosphate, to promote seed full.
The seed results: late June or early July,, fruit ear 2/3rds is seed maturity when becoming sepia, and can gather this moment, answers a ripe part results part, and receipts back airing is done back threshing in time on canvas or colour bar cloth.Should earlier the male parent acrial part be prescinded the seed of gathering in the crops again on the maternal plant before the results.
Strict production of hybrid seeds measure, it is pure to carry out anti-assorted guarantor
Seed farm will with the red sage root of other kind or salvia spacing distance more than 1000m, seedling stage and each impurity elimination of florescence 1 time simultaneously, the strain of mixing of assorted fringe is rejected in close inspection during results.Seed grinds to be beaten, in the airing process, note preventing artificially and mechanical admixture.
They red No. two breed standards
The biological property characteristic:
Phenophase: at the beginning of by the end of March to 4 months, emerge days red No. two, early or mid April bolting, mid or late April buddings, bloom at the beginning of 5 months, mid or late June seed maturity, late October the results medicinal material.
Morphological feature: day red No. two plant leafs are oval, and blade tip is point gradually, leaf edge sawtooth shape, blade face light green color, and stem stalk exocuticle is green and fine hair is arranged, the corolla purple, root is brick-red, the fruit sepia.
The overground part proterties: day red No. two plant height 40-60cm, the long 4.0-8.0cm of blade, wide 3.5-7.0cm, each petiole has 3-5 to go out compound leaf, and minority 7 goes out compound leaf, the long 12-20cm of inflorescence, the thick 1.2-1.4cm of stem, branch several months 12-14 then.
Root characteristic: day red No. two long 25-30cm of root; Root diameter 0.8-1.5cm; The root bar is counted 18-25 root/strain; Individual plant root fresh weight 260-550g/ strain; Individual plant root dry weight 80-120g/ strain; The rate 26.96% of giving money as a gift.
Quality and output: the bright red sage root 969.87-1071.38 of this kind per mu yield kilogram, amount to dried medicinal material 261.48-288.84 kilogram.Active constituent content: Tanshinone I I A content 0.56%, content of danshinolic acid B 7.4%.
The kind attribute: this article kind crossbreed, can only utilize the first-filial generation kind, two generation seed can not make kind of a usefulness.
Suitable area: suit in base, Shanglou and the whole nation similar area plantation.
Through red sage root regional experiment of variety; Identify the red sage root kind (being) of the seed selection red sage root and introduction; And adaptability and the productivity of landrace under different ecological condition of screening; Therefrom select wide adaptability, high yield, stable yields, high-quality, meet the good salvia kind of domestic and international market needs, for red sage root new varieties are identified, registration provides scientific basis.
Tested variety
The test tested variety adopts anonymous tubulation reason for hosting test unit, and unified numbering has 7 tested varieties, title and code name such as tables 1.Compare with Shanglou red sage root.
Table 1
| Code name | Variety name |
| 1 | It is red No. one |
| 2 | They red No. two |
| 3 | Shanglou red sage root |
| 4 | Wite red-rooted salvia root |
| 5 | The Shanxi red sage root |
| 6 | The Henan red sage root |
| 7 | The Shandong red sage root |
Experimental scheme
Randomised block design is adopted in test, three repetitions, and zone leader 6.0m, wide 3.6 meters, adopt the ridging plantation; 0.8 meter of row spacing, 0.3 meter of row spacing, every ridge plantation 3 row, line-spacing 25cm; Spacing in the rows 15cm, 3 ridges are planted in every sub-district, every ridge 120 young plants, density of reserved seedlings 16.5 ten thousand strains/hm2.The field is arranged and is seen table 2, representes with the code name in the table 1.
Table 2
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| 5 | 7 | 6 | 2 | 3 | 4 | 1 |
| 4 | 6 | 5 | 1 | 7 | 2 | 3 |
Profile test and field management
Experimental field field management is basic identical with general field management method, and fertilizing amount is a little more than the land for growing field crops, the soil property middle fertility.
Test result analysis
Main economic characters
Root is long
In the tested variety (being) average bright root root long the longest be days red No. two, be 28.30cm, average root long the shortest be Wite red-rooted salvia root, be 21.42cm, other kind (being) is between 23.49-28.18cm.
The root diameter
What average bright root root diameter was the thickest in the tested variety (being) is No. two, day pellet, is 1.37cm, and the thinnest is Wite red-rooted salvia root, is 0.76cm, and other kind (being) is between 0.99-1.21cm.
Root bar number
What average root bar number was maximum in the tested variety (being) is No. two, day pellet, is 21.47/strain, and minimum is the Shanxi red sage root, is 13.80/strain, and other kind (being) is between 14.31-17.16 root/strain.
Individual plant root fresh weight
What average individual plant root fresh weight weight was maximum in the tested variety (being) is No. two, day pellet, is the 428.64g/ strain, and minimum is Wite red-rooted salvia root, is the 265.11g/ strain, and other kind (being) is between the 322.77-375.44g/ strain.
The individual plant root dry weight
What average individual plant root dry weight weight was maximum in the tested variety (being) is No. two, day pellet, is the 114.42g/ strain, and minimum is Wite red-rooted salvia root, is the 71.31g/ strain, and other kind (being) is between the 83.94-102.63g/ strain.
The rate of giving money as a gift
What the rate of on average giving money as a gift in the tested variety (being) was maximum is No., day pellet, is 27.29%, and minimum is the Shandong red sage root, is 25.94%, and other kind (being) is between 26.00-26.96%.
Output
In the tested variety (being), what sub-district average root output of fresh was maximum is No. two, day pellet, is 33.47kg, per mu yield 1032.97kg, and minimum is Wite red-rooted salvia root, is 19.48kg, per mu yield 601.31kg, other kind (being) per mu yield is between 679.96-963.58kg.Through variance analysis, the article interspecific difference reaches significance level.
Each pilot average yield is the highest with Yang Ling, is 803.83kg, and adret is minimum, is 784.72kg.Through variance analysis, the volume variance of each pilot is not remarkable, shows that the environmental condition difference of each pilot is little.
Kind and place are made effect mutually and are not reached significance level, and yielding ability and the adaptability difference that shows each kind be significance not.Various places overall merit is: day red No. two kinds do very well, and a day kind of pellet shows better, and red sage root kind performance in Shanglou is general, and the Shanxi red sage root, the Henan red sage root, Shandong red sage root kind show relatively poor, and the Wite red-rooted salvia root kind shows bad.
Active constituent content
It red tanshinone IIA: 0.48%, tanshin polyphenolic acid B: 7.5%, contrast Shanglou red sage root (IIA:0.22%, tanshin polyphenolic acid B: 5.7%) increase by 118.18% and 32.73% respectively.They red No. two Tanshinone I I A:0.56%, tanshin polyphenolic acid B 7.4%, contrasting Shanglou red sage root increases by 154.55% and 29.82% respectively.
Kind (being) is commented
It red No., the long 28.19cm of root, root diameter 1.21cm, the several 17.16/strains of root bar, individual plant root fresh weight 375.44g, individual plant root dry weight 102.63g, the rate 27.29% of giving money as a gift.Average yield per mu (fresh weight) 963.58kg/ mu, comparison occupies the 2nd according to Shanglou red sage root raising the output 26.64%.General performance is good.
They red No. two, the long 28.30cm of root, root diameter 1.37cm, the several 21.47/strains of root bar, individual plant root fresh weight 428.64g, individual plant root dry weight 114.42g, the rate 26.96% of giving money as a gift.Average yield per mu (fresh weight) 1032.97g/ mu, comparison occupies the 1st according to Shanglou red sage root raising the output 35.76%.General performance is fine.
Shanglou red sage root, the long 25.87cm of root, root diameter 1..10cm, the several 16.62/strains of root bar, individual plant root fresh weight 338.67g, individual plant root dry weight 88.55g, the rate 26.66% of giving money as a gift.Average yield per mu (fresh weight) 760.90kg/ mu occupies the 3rd.General performance is general.
Wite red-rooted salvia root, the long 21.42cm of root, root diameter 0.76cm, the several 15.42/strains of root bar, individual plant root fresh weight 265.11g, individual plant root dry weight 71.31g, the rate 26.48% of giving money as a gift.Average yield per mu (fresh weight) 601.31g/ mu, comparison occupies the 7th according to Shanglou red sage root underproduction 20.97%.General performance is relatively poor.
The Shanxi red sage root, the long 24.16cm of root, root diameter 1.00cm, the several 13.80/strains of root bar, individual plant root fresh weight 324.24g, individual plant root dry weight 84.01g, the rate 26.16% of giving money as a gift.Average yield per mu (fresh weight) 679.96kg/ mu, comparison occupies the 6th according to Shanglou red sage root underproduction 10.64%.General performance is general.
The Henan red sage root, the long 24.53cm of root, root diameter 0.99cm, the several 16.46/strains of root bar, individual plant root fresh weight 329.23g, individual plant root dry weight 87.20g, the rate 26.00% of giving money as a gift.Average yield per mu (fresh weight) 713.68kg/ mu, comparison occupies the 5th according to Shanglou red sage root underproduction 6.21%.Performance is general.
The Shandong red sage root, the long 23.49cm of root, root diameter 1.02cm, the several 14.31/strains of root bar, individual plant root fresh weight 322.77g, individual plant root dry weight 83.94g, the rate 25.94% of giving money as a gift.Average yield per mu (fresh weight) 755.30kg/ mu, comparison occupies the 4th according to Shanglou red sage root underproduction 0.74%.General performance is general.
2010 is the 3rd year of red sage root regional test, through the joint efforts of each pilot, has obtained comparatively complete testing data, has further identified yield level, adaptability and the yield stability of tested variety (being).
Through variance analysis, tested variety, tested variety and pilot, pilot and between the time, pilot and tested variety all reach the significance level of difference.
In 3 annual red sage root regional experiment of variety, average per unit area yield surpasses contrast (Shanglou red sage root) be day red No. one with days red No. two, contrast raising the output 26.64% and 35.76% respectively.
Active constituent content show as day red No. one with days red No. two tanshinone IIA respectively contrast increase by 118.18% and 154.55%; Tanshin polyphenolic acid B is contrast increase by 32.73% and 29.82% respectively.
Concrete data in the above-mentioned experiment are shown in table 3-10:
The main economic characters summary sheet of table 32008-2010 red sage root regional experiment of variety
Table 42008-2010 red sage root regional experiment of variety yield result summary sheet
Table 5:2008-2010 red sage root regional experiment of variety kind, pilot output precedence summary sheet
Unit: kg/ mu
Table 6 red sage root regional experiment of variety variance analysis table (multiple years is district's group at random)
| Source of variation | SS | df | MS | F | Prob. |
| In the point between year inner region group | 91.4091 | 18 | 5.0783 | ||
| Between the time | 174.9207 | 2 | 87.4604 | 15.5951 | 0.0001 |
| Intersite | 245.515 | 2 | 122.7575 | 3.146 | 0.1511 |
| Between kind | 4100.438 | 6 | 683.4064 | 55.9275 | 0.0001 |
| Place * time | 156.0831 | 4 | 39.0208 | 6.9578 | 0.0001 |
| Kind * time | 146.6341 | 12 | 12.2195 | 2.1789 | 0.0176 |
| Place * kind | 219.5599 | 12 | 18.2967 | 1.363 | 0.2496 |
| The place x kind x time | 322.1819 | 24 | 13.4242 | 2.3937 | 0.0012 |
| Error | 605.6842 | 108 | 5.6082 | ||
| Total | 6062.426 | 188 |
Table 7 variety trial variance analysis table (multiple years is district's group at random)
| Source of variation | SS | df | MS | F | Prob. |
| In the point between year inner region group | 91.4091 | 18 | 5.0783 | ||
| Between the time | 174.9207 | 2 | 87.4604 | 15.5951 | 0.0001 |
| Intersite | 245.515 | 2 | 122.7575 | 3.146 | 0.1511 |
| Between kind | 4100.438 | 6 | 683.4064 | 55.9275 | 0.0001 |
| Place * time | 156.0831 | 4 | 39.0208 | 6.9578 | 0.0001 |
| Kind * time | 146.6341 | 12 | 12.2195 | 2.1789 | 0.0176 |
| Place * kind | 219.5599 | 12 | 18.2967 | 1.363 | 0.2496 |
| The place x kind x time | 322.1819 | 24 | 13.4242 | 2.3937 | 0.0012 |
| Error | 605.6842 | 108 | 5.6082 | ||
| Total | 6062.426 | 188 |
The multiple ratio of table 8Duncan ' s multiple range test
| The kind numbering | Average | 5% significance level | 1% utmost point significance level |
| 2 | 33.4667 | a | A |
| 1 | 31.2185 | b | A |
| 3 | 24.6519 | c | B |
| 7 | 24.4704 | c | B |
| 6 | 23.1222 | cd | B |
| 5 | 22.0296 | d | BC |
| 4 | 19.4815 | e | C |
Each intersite significance of difference multiple ratio of table 9
| The place | Average | 5% significance level | 1% utmost point significance level |
| Yang Ling | 27.1032 | a | A |
| Adret | 24.7079 | b | A |
| Shangzhou | 24.6635 | b | A |
Table 10 kind yielding ability and stability analysis thereof
The above; Be merely the preferable embodiment of the present invention; Protection scope of the present invention is not limited thereto; Any technical staff who is familiar with the present technique field is in the technical scope that the present invention discloses, and the simple change of the technical scheme that obtains or equivalence replacement all fall in protection scope of the present invention with may be obvious that.
Claims (8)
1. the selection of a red sage root new varieties is characterized in that, SH-B-18 does female parent with red sage root male sterile line, and fertile line red sage root A-107 does the formulated red sage root crossbreed of male parent.
2. the selection of red sage root new varieties according to claim 1 is characterized in that, at the beginning of 4 months, emerges at the bottom of the said maternal red sage root male sterile line SH-B-183 month; Mid-April bolting, late April buddings, the early flowering season at the beginning of 5 months; May the early and middle ten days full-bloom stage; Seed maturity at the beginning of late June to 7 month, late October the medicinal material of gathering, mid or late November is withered.
3. the selection of red sage root new varieties according to claim 1 is characterized in that, digs out kind of a root before said maternal red sage root male sterile line SH-B-18 late October or soil are freeze-up, is cut into the 5-8cm segment; On hold level with both hands, the lower end is oblique, ground is turned over, is flattened, 20 kilograms/mu of sealing fertilizers; Dig dark 20cm, the little furrow of wide 100-120cm, the long 10-12m of furrow makes the bottom, seedbed loose smooth; In soil in the root segment straight cutting furrow, and keep the upper end upwards, lower ends downward, spacing in the rows 3-5cm; Line-spacing 5-10cm buries with wet soil and to cut root, and wet soil thickness and furrow face are closely flat.
4. the selection of red sage root new varieties according to claim 1 is characterized in that, said maternal red sage root male sterile line SH-B-18 can transplant when seedling grows 4 young leaves, and seeding row spacing is 20cm * 25cm.
5. the selection of red sage root new varieties according to claim 1 is characterized in that, at the beginning of 4 months, emerges at the bottom of the said male parent fertile line red sage root A-1073 month; Mid-April bolting, late April buddings, the early flowering season at the beginning of 5 months; May the early and middle ten days full-bloom stage; Seed maturity at the beginning of late June to 7 month, late October the medicinal material of gathering, mid or late November is withered.
6. the selection of red sage root new varieties according to claim 1 is characterized in that, the said male parent fertile line red sage root is when the A-1077 month, early and middle ten days was grown seedlings; Earlier ground is turned over, flattened, 20 kilograms/mu of sealing fertilizers, rake is thin; Do the wide little furrow of wide 100-120cm, the drilling of ditching again, ditch depth 2-4cm.
7. the selection of red sage root new varieties according to claim 1 is characterized in that, said male parent fertile line red sage root A-107 treats that shoot root is long long and can transplant to 5-10cm that seeding row spacing is 20cm * 25cm.
8. the selection of red sage root new varieties according to claim 1 is characterized in that, this capable by 1: 2 or 2: 2 of father and mother during the breeding of said red sage root crossbreed than planting, and seeding row spacing is 20cm * 25cm.
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Cited By (6)
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| CN103583367A (en) * | 2013-11-20 | 2014-02-19 | 陈瑞阳 | Quick breeding method for novel triploid salvia miltiorrhiza detoxification varieties |
| CN105724238A (en) * | 2016-02-24 | 2016-07-06 | 河南中医学院 | Good-quality and high-yield salvia miltiorrhiza cultivation method |
| CN107173008A (en) * | 2017-06-07 | 2017-09-19 | 云南自然谷生物开发有限公司 | Improve the red sage root implantation methods of tanshinone content |
| CN108633660A (en) * | 2018-04-27 | 2018-10-12 | 山东省农业科学院农产品研究所 | A kind of Radix Salviae Miltiorrhizae new varieties production of hybrid seeds method for culturing seedlings |
| CN113796271A (en) * | 2020-06-15 | 2021-12-17 | 山东农业大学 | Method for rapidly breeding excellent red sage root variety |
| CN116472957A (en) * | 2023-03-07 | 2023-07-25 | 四川农业大学 | Method for screening nuclear-cytoplasmic interactive male sterile red sage root and application thereof |
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|---|---|---|---|---|
| CN103583367A (en) * | 2013-11-20 | 2014-02-19 | 陈瑞阳 | Quick breeding method for novel triploid salvia miltiorrhiza detoxification varieties |
| CN103583367B (en) * | 2013-11-20 | 2015-07-15 | 陈瑞阳 | Quick breeding method for novel triploid salvia miltiorrhiza detoxification varieties |
| CN105724238A (en) * | 2016-02-24 | 2016-07-06 | 河南中医学院 | Good-quality and high-yield salvia miltiorrhiza cultivation method |
| CN105724238B (en) * | 2016-02-24 | 2018-05-29 | 河南中医学院 | A kind of breeding method of good quality and high output Radix Salviae Miltiorrhizae |
| CN107173008A (en) * | 2017-06-07 | 2017-09-19 | 云南自然谷生物开发有限公司 | Improve the red sage root implantation methods of tanshinone content |
| CN108633660A (en) * | 2018-04-27 | 2018-10-12 | 山东省农业科学院农产品研究所 | A kind of Radix Salviae Miltiorrhizae new varieties production of hybrid seeds method for culturing seedlings |
| CN113796271A (en) * | 2020-06-15 | 2021-12-17 | 山东农业大学 | Method for rapidly breeding excellent red sage root variety |
| CN116472957A (en) * | 2023-03-07 | 2023-07-25 | 四川农业大学 | Method for screening nuclear-cytoplasmic interactive male sterile red sage root and application thereof |
| CN116472957B (en) * | 2023-03-07 | 2024-02-02 | 四川农业大学 | Method for screening nuclear-cytoplasmic interactive male sterile red sage root and application thereof |
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