CN105294254A - Crop nutrient based on hydrolyzed selenium-enriched yeast and preparation method and application of crop nutrient - Google Patents
Crop nutrient based on hydrolyzed selenium-enriched yeast and preparation method and application of crop nutrient Download PDFInfo
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- CN105294254A CN105294254A CN201510718115.9A CN201510718115A CN105294254A CN 105294254 A CN105294254 A CN 105294254A CN 201510718115 A CN201510718115 A CN 201510718115A CN 105294254 A CN105294254 A CN 105294254A
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Abstract
The invention discloses crop nutrient based on hydrolyzed selenium-enriched yeast and a preparation method and application of the crop nutrient. According to the method, sodium selenite serves as a selenium source, a fermentation method is adopted for culturing selenium-enriched saccharomycetes, fermentation raw materials and water do not contain sulphur-containing substances, then the selenium-enriched saccharomycetes is subjected to hydrolytic treatment through pig gastric mucosa, and thus the crop nutrient is obtained. The invention provides the crop nutrient produced by the preparation method of the crop nutrient based on hydrolyzed selenium-enriched yeast and application of the crop nutrient. According to the preparation method, all raw materials are fully and reasonably utilized, 'thorough utilization' is achieved, no residual slag or residual liquor exists, and no environmental pollution exists; the obtained crop nutrient is high in content of organic selenium, the organic selenium exists in a selenium substituted amino acid form, absorption of plants is facilitated, and the content of organic selenium in agricultural products can be remarkably raised; the crop nutrient can be subjected to spraying application or root application according to the selected products in actual situations.
Description
Technical field
The present invention relates to organic selenium fertilizer field.More particularly, the present invention relates to a kind of corps nutrient thing based on hydrolysis yeast rich in selenium and preparation method thereof and application.
Background technology
Lack selenium element in the soil of China more than 60%, therefore the general selenium content of agricultural-food is not enough.According to " DRIs " that China formulates for 2000, within more than 18 years old, grownup's selenium intake every day should be more than 50 micrograms (50 μ g/d), the average daily intake that the World Health Organization (WHO) is recommended is 50 ~ 200 μ g/d, but China resident actual average intake is only 27 micrograms.Some area of China extremely lacks selenium, and situation is even more serious.Selenium plays anti-cancer, anti-oxidant and improve the vital role of immunological competence in human body, and the selenium content therefore promoting agricultural products in China is the important measures improving people ' s health.
Current farm crop are mainly fertile by the blade sprayed containing Sodium Selenite, to increase selenium constituent content in agricultural-food.In fodder industry, mainly through adding Sodium Selenite, increase the selenium constituent content in meat, egg.But to human body it is beneficial that organoselenium (effective constituent is selenomethionine, selenocystine, seleno-cysteine etc.), inorganic selenium (sodium selenate and Sodium Selenite) on the contrary can the health of harmful to human.There is strict limit standard in country to inorganic selenium content in agricultural-food, as in cereals, inorganic selenium content can not more than 0.3mg/kg.The absorbent organoselenium of crop leaf is seleno-amino acids (selenomethionine, selenocystine, seleno-cysteine etc.), and existing organoselenium crop leaf spray product can not ensure that selenium element exists with the form of seleno-amino acids.Therefore be necessary to develop the novel crops nutrition being rich in organoselenium, to promote the organic selenium content in agricultural-food.
Summary of the invention
An object of the present invention is to solve at least the problems referred to above, and the advantage will illustrated at least is below provided.
A further object of the invention is to provide a kind of corps nutrient thing based on hydrolysis yeast rich in selenium, and in this nutrition, selenium content is high, and exists with the form of seleno-amino acids, is conducive to the absorption of crop, can significantly improve the content of organoselenium in agricultural-food.
A further object of the invention is to provide a kind of preparation method of the corps nutrient thing based on hydrolysis yeast rich in selenium, the method take Sodium Selenite as selenium source, fermentation method is adopted to cultivate yeast rich in selenium, not containing S-contained substance in fermentation substrate, selenium content in yeast is high, again yeast rich in selenium is hydrolyzed process, and then obtained described corps nutrient thing.
In order to realize according to these objects of the present invention and other advantage, provide a kind of preparation method of the corps nutrient thing based on hydrolysis yeast rich in selenium, comprise yeast rich in selenium and cultivate, it comprises the steps:
Steps A, the tapioca (flour) of the urea of the molasses of 3-8 weight part, 3-8 weight part, the dregs of beans of 3-9 weight part and 4-12 weight part is stirred 35min at 110-130 DEG C, obtain sterilizing raw material;
Step B, when described sterilizing material temperature is down to below 35 DEG C, adds the Sodium Selenite of 0.05-0.3 weight part, the potassiumphosphate of 1-3 weight part and the magnesium chloride of 0.5-1.5 weight part, under the rotating speed of 100-200r/min, stir 1h, obtain fermentation substrate;
Step C, to Air washer in described fermentation substrate to fermentation substrate water content be 20-40%, add the cereuisiae fermentum of 2-3 weight part, stir 1h under the rotating speed of 100-200r/min after, continue to Air washer in described fermentation substrate, make its water content reach 50-70%, more described fermentation substrate to be placed in temperature be 35-40 DEG C, humidity is the condition bottom fermentation 36-60h of 85-90%, obtain yeast rich in selenium mixture, with 150r/min Keep agitation in fermenting process.
Preferably, the preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, also comprises, described yeast rich in selenium mixture is stirred 30min at 110-130 DEG C and carries out sterilizing, carry out hydrolysis treatment, obtain hydrolysed mix after sterilizing; By described hydrolysed mix after filtration or dry, described corps nutrient thing is obtained.
Preferably, the preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described hydrolysis treatment, it joins in the described yeast rich in selenium mixture after sterilizing after the pig gastric mucosa of 1-2 weight part is ground to form pasty state, adds water, the water content of described yeast rich in selenium mixture is regulated to be 70-90%, and adjust its pH value to 3-4 with hydrochloric acid, in 37 DEG C, under the rotating speed of 150r/min, hydrolysis 4-6h, is adjusted to 6.5-7.5 by its pH value after being hydrolyzed.
Preferably, the preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described hydrolysis treatment, its be by sterilizing after described yeast rich in selenium mixture carry out Microwave Pretreatment, microwave condition is: frequency 300-500MHz, time 30-45s, join after again the pig gastric mucosa of 1-2 weight part being ground to form pasty state in pretreated described yeast rich in selenium mixture, add water, the water content of described yeast rich in selenium mixture is regulated to be 80-90%, and adjust its pH value to 3-4 with hydrochloric acid, transformation hydrolysis 4h at 30-35 DEG C, wherein, when 0-1h and 2-4h, pressure is 0.1MPa, when 1-2h, pressure is 0.12-0.13MPa, after being hydrolyzed, its pH value is adjusted to 6.5-7.5.
Preferably, the preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described corps nutrient thing comprises yeast rich in selenium base crop nutrient solution, the corps nutrient agent of yeast rich in selenium base and yeast rich in selenium base corps nutrient material, wherein, described yeast rich in selenium base crop nutrient solution and the agent of described yeast rich in selenium base corps nutrient, it is respectively the pure water adding 80-120 weight part in described hydrolysed mix, after mixing, the filtrate obtained after filtration and filter residue; Described yeast rich in selenium base corps nutrient material, it described hydrolysed mix is stirred under the rotating speed of 50-60 DEG C, 200r/min dry 5-6h to obtain.
Preferably, the preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, in described steps A, described molasses are 5 weight parts, and described urea is 5 weight parts, and described dregs of beans is 6 weight parts, and described tapioca (flour) is 8 weight parts; In described step B, described Sodium Selenite is 0.1-0.2 weight part, and described potassiumphosphate is 2 weight parts, and described magnesium chloride is 1 weight part.
Preferably, the preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, in described step C, described fermentation substrate being placed in temperature is 37 DEG C, and humidity is the condition bottom fermentation 48h of 85%, obtains yeast rich in selenium mixture.
Preferably, the preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described pig gastric mucosa collects the fresh pig gastric mucosa in slaughterhouse, by after its packing in-30 DEG C of freezings, during use, described pig gastric mucosa is placed in container, places at 4-7 DEG C, after thawing completely, use after described pig gastric mucosa is ground to form pasty state together with the remaining juice in container.
A kind of corps nutrient thing of preparation method's production of the corps nutrient thing based on hydrolysis yeast rich in selenium.
An application for corps nutrient thing, described yeast rich in selenium base crop nutrient solution, its through dilution 200-300 doubly after for foliage-spray; Described yeast rich in selenium base corps nutrient agent and described yeast rich in selenium base corps nutrient material, it is directly used in soil or crop cultivation matrix, and in soil, consumption is 30-80 kg/acre, and in crop cultivation matrix, addition is 0.5-5%.
The present invention at least comprises following beneficial effect:
The first, the present inventor finds, in yeast rich in selenium culturing process, exist between sulphur and selenium and vie each other, the existence of sulphur can affect the absorption of yeast to selenium element, reduces the organic selenium content in yeast; In yeast rich in selenium culturing process, do not use raw material and the water of sulfur-bearing, be conducive to the abundant absorption of selenium to the replacement of sulphur and selenium element, improve the content of organoselenium in yeast;
The second, adopt pig gastric mucosa hydrolysis yeast rich in selenium, ensure that the organoselenium in product exists with the form of seleno-amino acids, be conducive to the absorption of plant, the content of organoselenium in agricultural-food can be significantly improved, and pig gastric mucosa price is low, hydrolysising condition is gentle, can effectively reduce hydrolysis cost;
Three, the corps nutrient thing adopting the present invention to prepare, not only overcomes inorganic selenium in agricultural-food and, to the harm of human body, also overcomes the fertilizer damage phenomenon such as burning seedling, tikka caused because the fertile consumption of inorganic selenium is improper;
Four, before inoculation yeast bacterial classification, by molasses, urea, dregs of beans and tapioca (flour) are carried out sufficient germicidal treatment, the growth of miscellaneous bacteria can be avoided; After adding Sodium Selenite, potassiumphosphate and magnesium chloride, by abundant stirring, the excessive injury to barms in the local of Sodium Selenite can be avoided;
Five, by the described yeast rich in selenium mixture after sterilizing being carried out Microwave Pretreatment and transformation hydrolysis, hydrolysis efficiency and seleno-amino acids content can be improved, wherein, Microwave Pretreatment, the slight cracking of yeast rich in selenium albumen can be made, increase the action site of enzyme in pig gastric mucosa; Increasing hydrolysis pressure when being hydrolyzed 1-2h to 0.12-0.13MPa, the activity of enzyme in pig gastric mucosa can be improved;
Six, preparation method of the present invention, makes all raw materials all obtain and fully, reasonably utilizes, and achieves " eating dry squeezing only ", does not occur any residue, raffinate, without any environmental pollution.
Part is embodied by explanation below by other advantage of the present invention, target and feature, part also will by research and practice of the present invention by those skilled in the art is understood.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, can implement according to this with reference to specification sheets word to make those skilled in the art.
It should be noted that experimental technique described in following embodiment if no special instructions, is ordinary method, described reagent and material, if no special instructions, all can obtain from commercial channels.
Embodiment 1:
Based on a preparation method for the corps nutrient thing of hydrolysis yeast rich in selenium, comprise yeast rich in selenium and cultivate, it comprises the steps:
Steps A, the tapioca (flour) of the urea of the molasses of 3 weight parts, 3 weight parts, the dregs of beans of 3 weight parts and 4 weight parts is stirred 35min at 110 DEG C, obtain sterilizing raw material;
Step B, when described sterilizing material temperature is down to below 35 DEG C, adds the Sodium Selenite of 0.05 weight part, the potassiumphosphate of 1 weight part and the magnesium chloride of 0.5 weight part, under the rotating speed of 100r/min, stir 1h, obtain fermentation substrate;
Step C, to Air washer in described fermentation substrate to fermentation substrate water content be 20%, add the cereuisiae fermentum of 2 weight parts, stir 1h under the rotating speed of 100r/min after, continue to Air washer in described fermentation substrate, make its water content reach 50%, more described fermentation substrate to be placed in temperature be 35 DEG C, humidity is the condition bottom fermentation 60h of 85%, obtain yeast rich in selenium mixture, with 150r/min Keep agitation in fermenting process.
In above-mentioned raw materials, part material can use other raw material substitutions, and as potassium hydrogen phosphate sodium phosphate+Repone K substitutes, Sodium Selenite sodium selenate substitutes, molasses sucrose or glucose substitute, other dregs of fat of dregs of beans substitute; For promoting that in fermenting process, yeast is to the absorption of selenium element, in raw material and water, all do not contain S-contained substance.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, also comprises, described yeast rich in selenium mixture is stirred 30min at 110 DEG C and carries out sterilizing, carry out hydrolysis treatment, obtain hydrolysed mix after sterilizing; By described hydrolysed mix after filtration or dry, described corps nutrient thing is obtained.
After having fermented, yeast rich in selenium mixture is carried out sterilising treatment, can constant product quality be ensured.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described hydrolysis treatment, it joins in the described yeast rich in selenium mixture after sterilizing after the pig gastric mucosa of 1 weight part is ground to form pasty state, add water, regulate the water content of described yeast rich in selenium mixture to be 70%, and adjust its pH value to 3 with hydrochloric acid, in 37 DEG C, under the rotating speed of 150r/min, hydrolysis 4h, is adjusted to 6.5 by its pH value after being hydrolyzed.
Described pig gastric mucosa can with other acidic protein enzymes extraction.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described corps nutrient thing comprises yeast rich in selenium base crop nutrient solution, the corps nutrient agent of yeast rich in selenium base and yeast rich in selenium base corps nutrient material, wherein, described yeast rich in selenium base crop nutrient solution and the agent of described yeast rich in selenium base corps nutrient, it is respectively the pure water adding 80 weight parts in described hydrolysed mix, after mixing, and the filtrate obtained after filtration and filter residue; Described yeast rich in selenium base corps nutrient material, it is by described hydrolysed mix through 50 DEG C, stirs dry 5h and obtain under the rotating speed of 200r/min.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described pig gastric mucosa collects the fresh pig gastric mucosa in slaughterhouse, by after its packing in-30 DEG C of freezings, during use, described pig gastric mucosa is placed in container, place at 4 DEG C, after thawing completely, use after described pig gastric mucosa is ground to form pasty state together with the remaining juice in container.
A kind of corps nutrient thing of preparation method's production of the corps nutrient thing based on hydrolysis yeast rich in selenium.
An application for corps nutrient thing,
Described yeast rich in selenium base crop nutrient solution, its organic selenium content is 0.24g/L, sprays the peanut blade face in Post flowering after being diluted 200 times, sprayed once every 7 days, totally three times, after gathering, detect organic selenium content, reach 0.13mg/kg, than the high 200-300 do not used doubly;
The agent of described yeast rich in selenium base corps nutrient, its organic selenium content is 0.16g/kg, and added in soil before peanut seeding, every mu of addition is 30 kilograms, detects organic selenium content, reach 0.08mg/kg after gathering, than the high 120-180 do not used doubly;
Described yeast rich in selenium base corps nutrient material, its organic selenium content is 0.35g/kg, and added in soil before peanut seeding, every mu of addition is 30 kilograms, detects organic selenium content, reach 0.26mg/kg after gathering, than the high 400-600 do not used doubly.
Embodiment 2:
Based on a preparation method for the corps nutrient thing of hydrolysis yeast rich in selenium, comprise yeast rich in selenium and cultivate, it comprises the steps:
Steps A, the tapioca (flour) of the urea of the molasses of 5 weight parts, 5 weight parts, the dregs of beans of 6 weight parts and 8 weight parts is stirred 35min at 120 DEG C, obtain sterilizing raw material;
Step B, when described sterilizing material temperature is down to below 35 DEG C, adds the Sodium Selenite of 0.1 weight part, the potassiumphosphate of 2 weight parts and the magnesium chloride of 1 weight part, under the rotating speed of 150r/min, stir 1h, obtain fermentation substrate;
Step C, to Air washer in described fermentation substrate to fermentation substrate water content be 30%, add the cereuisiae fermentum of 2.5 weight parts, stir 1h under the rotating speed of 150r/min after, continue to Air washer in described fermentation substrate, make its water content reach 60%, more described fermentation substrate to be placed in temperature be 37 DEG C, humidity is the condition bottom fermentation 48h of 85%, obtain yeast rich in selenium mixture, with 150r/min Keep agitation in fermenting process.
In above-mentioned raw materials, part material can use other raw material substitutions, and as potassium hydrogen phosphate sodium phosphate+Repone K substitutes, Sodium Selenite sodium selenate substitutes, molasses sucrose or glucose substitute, other dregs of fat of dregs of beans substitute; For promoting that in fermenting process, yeast is to the absorption of selenium element, in raw material and water, all do not contain S-contained substance.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, also comprises, described yeast rich in selenium mixture is stirred 30min at 120 DEG C and carries out sterilizing, carry out hydrolysis treatment, obtain hydrolysed mix after sterilizing; By described hydrolysed mix after filtration or dry, described corps nutrient thing is obtained.
After having fermented, yeast rich in selenium mixture is carried out sterilising treatment, can constant product quality be ensured.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described hydrolysis treatment, it joins in the described yeast rich in selenium mixture after sterilizing after the pig gastric mucosa of 1.5 weight parts is ground to form pasty state, add water, regulate the water content of described yeast rich in selenium mixture to be 80%, and adjust its pH value to 3.5 with hydrochloric acid, in 37 DEG C, under the rotating speed of 150r/min, hydrolysis 5h, is adjusted to 7 by its pH value after being hydrolyzed.
Described pig gastric mucosa can with other acidic protein enzymes extraction.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described corps nutrient thing comprises yeast rich in selenium base crop nutrient solution, the corps nutrient agent of yeast rich in selenium base and yeast rich in selenium base corps nutrient material, wherein, described yeast rich in selenium base crop nutrient solution and the agent of described yeast rich in selenium base corps nutrient, it is respectively the pure water adding 100 weight parts in described hydrolysed mix, after mixing, and the filtrate obtained after filtration and filter residue; Described yeast rich in selenium base corps nutrient material, it is by described hydrolysed mix through 55 DEG C, stirs dry 5.5h and obtain under the rotating speed of 200r/min.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described pig gastric mucosa collects the fresh pig gastric mucosa in slaughterhouse, by after its packing in-30 DEG C of freezings, during use, described pig gastric mucosa is placed in container, place at 5 DEG C, after thawing completely, use after described pig gastric mucosa is ground to form pasty state together with the remaining juice in container.
A kind of corps nutrient thing of preparation method's production of the corps nutrient thing based on hydrolysis yeast rich in selenium.
An application for corps nutrient thing,
Described yeast rich in selenium base crop nutrient solution, its organic selenium content is 0.3g/L, spray in oyster mushroom, Pleurotus eryngii bacterium, flat mushroom bacterium, Coprinus comatus bacterium the last week of gathering after being diluted 250 times, once a day, continuous three days, detect organic selenium content after gathering 3 days, reach 25-30 μ g/kg (dry weight), higher 100 ~ 200 times than what do not use;
The agent of described yeast rich in selenium base corps nutrient, its organic selenium content is 0.2g/kg, added in edible fungus culture medium, addition is 1%, for cultivating oyster mushroom, Pleurotus eryngii bacterium, flat mushroom bacterium, Coprinus comatus bacterium, detect organic selenium content after gathering 3 days, reach 22-28 μ g/kg (dry weight), higher 80 ~ 150 times than what do not use;
Described yeast rich in selenium base corps nutrient material, its organic selenium content is 0.45g/kg, added in edible fungus culture medium, addition is 0.67%, for cultivating oyster mushroom, Pleurotus eryngii bacterium, flat mushroom bacterium, Coprinus comatus bacterium, detect organic selenium content after gathering 3 days, reach 30-35 μ g/kg (dry weight), than the high 200-300 do not used doubly.
Embodiment 3:
On the basis of embodiment 2, described hydrolysis treatment, its be by sterilizing after described yeast rich in selenium mixture carry out Microwave Pretreatment, microwave condition is: frequency 400MHz, time 35s, join after again the pig gastric mucosa of 1.5 weight parts being ground to form pasty state in pretreated described yeast rich in selenium mixture, add water, the water content of described yeast rich in selenium mixture is regulated to be 80%, and adjust its pH value to 3.5 with hydrochloric acid, transformation hydrolysis 4h at 32 DEG C, wherein, when 0-1h and 2-4h, pressure is 0.1MPa, when 1-2h, pressure is 0.125MPa, after being hydrolyzed, its pH value is adjusted to 7.0.
An application for corps nutrient thing,
Described yeast rich in selenium base crop nutrient solution, its organic selenium content is 0.36g/L, spray in oyster mushroom, Pleurotus eryngii bacterium, flat mushroom bacterium, Coprinus comatus bacterium the last week of gathering after being diluted 250 times, once a day, continuous three days, detect organic selenium content after gathering 3 days, reach 29-34 μ g/kg (dry weight), than the high 115-220 do not used doubly;
The agent of described yeast rich in selenium base corps nutrient, its organic selenium content is 0.24g/kg, added in edible fungus culture medium, addition is 1%, for cultivating oyster mushroom, Pleurotus eryngii bacterium, flat mushroom bacterium, Coprinus comatus bacterium, detect organic selenium content after gathering 3 days, reach 26-33 μ g/kg (dry weight), than the high 95-180 do not used doubly;
Described yeast rich in selenium base corps nutrient material, its organic selenium content is 0.54g/kg, added in edible fungus culture medium, addition is 0.67%, for cultivating oyster mushroom, Pleurotus eryngii bacterium, flat mushroom bacterium, Coprinus comatus bacterium, detect organic selenium content after gathering 3 days, reach 35-41 μ g/kg (dry weight), than the high 235-350 do not used doubly.
Embodiment 4:
Based on a preparation method for the corps nutrient thing of hydrolysis yeast rich in selenium, comprise yeast rich in selenium and cultivate, it comprises the steps:
Steps A, the tapioca (flour) of the urea of the molasses of 8 weight parts, 8 weight parts, the dregs of beans of 9 weight parts and 12 weight parts is stirred 35min at 130 DEG C, obtain sterilizing raw material;
Step B, when described sterilizing material temperature is down to below 35 DEG C, adds the Sodium Selenite of 0.3 weight part, the potassiumphosphate of 3 weight parts and the magnesium chloride of 1.5 weight parts, under the rotating speed of 200r/min, stir 1h, obtain fermentation substrate;
Step C, to Air washer in described fermentation substrate to fermentation substrate water content be 40%, add the cereuisiae fermentum of 3 weight parts, stir 1h under the rotating speed of 200r/min after, continue to Air washer in described fermentation substrate, make its water content reach 70%, more described fermentation substrate to be placed in temperature be 40 DEG C, humidity is the condition bottom fermentation 36h of 90%, obtain yeast rich in selenium mixture, with 150r/min Keep agitation in fermenting process.
In above-mentioned raw materials, part material can use other raw material substitutions, and as potassium hydrogen phosphate sodium phosphate+Repone K substitutes, Sodium Selenite sodium selenate substitutes, molasses sucrose or glucose substitute, other dregs of fat of dregs of beans substitute; For promoting that in fermenting process, yeast is to the absorption of selenium element, in raw material and water, all do not contain S-contained substance.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, also comprises, described yeast rich in selenium mixture is stirred 30min at 130 DEG C and carries out sterilizing, carry out hydrolysis treatment, obtain hydrolysed mix after sterilizing; By described hydrolysed mix after filtration or dry, described corps nutrient thing is obtained.
After having fermented, yeast rich in selenium mixture is carried out sterilising treatment, can constant product quality be ensured.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described hydrolysis treatment, it joins in the described yeast rich in selenium mixture after sterilizing after the pig gastric mucosa of 2 weight parts is ground to form pasty state, add water, regulate the water content of described yeast rich in selenium mixture to be 90%, and adjust its pH value to 4 with hydrochloric acid, in 37 DEG C, under the rotating speed of 150r/min, hydrolysis 6h, is adjusted to 7.5 by its pH value after being hydrolyzed.
Described pig gastric mucosa can with other acidic protein enzymes extraction.
The preparation method of the described corps nutrient thing based on hydrolysis yeast rich in selenium, described corps nutrient thing comprises yeast rich in selenium base crop nutrient solution, the corps nutrient agent of yeast rich in selenium base and yeast rich in selenium base corps nutrient material, wherein, described yeast rich in selenium base crop nutrient solution and the agent of described yeast rich in selenium base corps nutrient, it is respectively the pure water adding 120 weight parts in described hydrolysed mix, after mixing, and the filtrate obtained after filtration and filter residue; Described yeast rich in selenium base corps nutrient material, it is by described hydrolysed mix through 60 DEG C, stirs dry 6h and obtain under the rotating speed of 200r/min.
A kind of corps nutrient thing of preparation method's production of the corps nutrient thing based on hydrolysis yeast rich in selenium.
Described yeast rich in selenium base crop nutrient solution, its organic selenium content is 0.23g/L, starts to spray after being diluted 300 times in the paddy rice earing flowering initial stage, sprayed once every 5 days, totally three times, after gathering, detect organic selenium content, reach 0.25mg/kg, than the high 150-200 do not used doubly;
The agent of described yeast rich in selenium base corps nutrient, its organic selenium content is 0.14g/kg, and added in soil before peanut seeding, every mu of addition is 80 kilograms, detects organic selenium content, reach 0.1mg/kg after gathering, than the high 60-80 do not used doubly;
Described yeast rich in selenium base corps nutrient material, its organic selenium content is 0.4g/kg, and added in soil before peanut seeding, every mu of addition is 80 kilograms, detects organic selenium content, reach 0.29mg/kg after gathering, than the high 200-250 do not used doubly.
Comparative example 1:
The beneficial effect using the raw material of not sulfur-bearing cultivation yeast rich in selenium to prepare corps nutrient thing is further illustrated in the present invention by comparative example 1.Wherein, control group is the potassium sulfate potassiumphosphate of 2 weight parts in embodiment 2 step B being replaced with 2 weight parts, the composition of all the other raw materials of control group, proportioning, and operational condition is with embodiment 2.Experimental result sees the following form.
As seen from the above table, in control group, organic selenium content in the yeast rich in selenium base crop nutrient solution of gained, the corps nutrient agent of yeast rich in selenium base and yeast rich in selenium base corps nutrient material is all remarkable in embodiment 2, illustrate that in the existence meeting remarkably influenced yeast rich in selenium culturing process of the sulfate ion in potassium sulfate, cereuisiae fermentum is to the absorption of selenium, cause the organic selenium content in yeast to reduce, and then the organic selenium content in the yeast rich in selenium base crop nutrient solution making to obtain, the corps nutrient agent of yeast rich in selenium base and yeast rich in selenium base corps nutrient material reduce; In yeast rich in selenium culturing process, do not use raw material and the water of sulfur-bearing, be conducive to the abundant absorption of selenium to the replacement of sulphur and selenium element, improve the content of organoselenium in yeast.
Although embodiment of the present invention are open as above, but it is not restricted to listed in specification sheets and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (10)
1. based on a preparation method for the corps nutrient thing of hydrolysis yeast rich in selenium, it is characterized in that, comprise yeast rich in selenium and cultivate, it comprises the steps:
Steps A, the tapioca (flour) of the urea of the molasses of 3-8 weight part, 3-8 weight part, the dregs of beans of 3-9 weight part and 4-12 weight part is stirred 35min at 110-130 DEG C, obtain sterilizing raw material;
Step B, when described sterilizing material temperature is down to below 35 DEG C, adds the Sodium Selenite of 0.05-0.3 weight part, the potassiumphosphate of 1-3 weight part and the magnesium chloride of 0.5-1.5 weight part, under the rotating speed of 100-200r/min, stir 1h, obtain fermentation substrate;
Step C, to Air washer in described fermentation substrate to fermentation substrate water content be 20-40%, add the cereuisiae fermentum of 2-3 weight part, stir 1h under the rotating speed of 100-200r/min after, continue to Air washer in described fermentation substrate, make its water content reach 50-70%, more described fermentation substrate to be placed in temperature be 35-40 DEG C, humidity is the condition bottom fermentation 36-60h of 85-90%, obtain yeast rich in selenium mixture, with 150r/min Keep agitation in fermenting process.
2. as claimed in claim 1 based on the preparation method of the corps nutrient thing of hydrolysis yeast rich in selenium, it is characterized in that, also comprise, described yeast rich in selenium mixture is stirred 30min at 110-130 DEG C and carries out sterilizing, carry out hydrolysis treatment after sterilizing, obtain hydrolysed mix; By described hydrolysed mix after filtration or dry, described corps nutrient thing is obtained.
3. as claimed in claim 2 based on the preparation method of the corps nutrient thing of hydrolysis yeast rich in selenium, it is characterized in that, described hydrolysis treatment, it joins in the described yeast rich in selenium mixture after sterilizing after the pig gastric mucosa of 1-2 weight part is ground to form pasty state, adds water, the water content of described yeast rich in selenium mixture is regulated to be 70-90%, and adjust its pH value to 3-4 with hydrochloric acid, in 37 DEG C, under the rotating speed of 150r/min, hydrolysis 4-6h, is adjusted to 6.5-7.5 by its pH value after being hydrolyzed.
4. as claimed in claim 2 based on the preparation method of the corps nutrient thing of hydrolysis yeast rich in selenium, it is characterized in that, described hydrolysis treatment, its be by sterilizing after described yeast rich in selenium mixture carry out Microwave Pretreatment, microwave condition is: frequency 300-500MHz, time 30-45s, join after again the pig gastric mucosa of 1-2 weight part being ground to form pasty state in pretreated described yeast rich in selenium mixture, add water, the water content of described yeast rich in selenium mixture is regulated to be 80-90%, and adjust its pH value to 3-4 with hydrochloric acid, transformation hydrolysis 4h at 30-35 DEG C, wherein, when 0-1h and 2-4h, pressure is 0.1MPa, when 1-2h, pressure is 0.12-0.13MPa, after being hydrolyzed, its pH value is adjusted to 6.5-7.5.
5. as claimed in claim 2 based on the preparation method of the corps nutrient thing of hydrolysis yeast rich in selenium, it is characterized in that, described corps nutrient thing comprises yeast rich in selenium base crop nutrient solution, the corps nutrient agent of yeast rich in selenium base and yeast rich in selenium base corps nutrient material, wherein, described yeast rich in selenium base crop nutrient solution and the agent of described yeast rich in selenium base corps nutrient, it is respectively the pure water adding 80-120 weight part in described hydrolysed mix, after mixing, the filtrate obtained after filtration and filter residue; Described yeast rich in selenium base corps nutrient material, it described hydrolysed mix is stirred under the rotating speed of 50-60 DEG C, 200r/min dry 5-6h to obtain.
6., as claimed in claim 1 based on the preparation method of the corps nutrient thing of hydrolysis yeast rich in selenium, it is characterized in that, in described steps A, described molasses are 5 weight parts, described urea is 5 weight parts, and described dregs of beans is 6 weight parts, and described tapioca (flour) is 8 weight parts; In described step B, described Sodium Selenite is 0.1-0.2 weight part, and described potassiumphosphate is 2 weight parts, and described magnesium chloride is 1 weight part.
7., as claimed in claim 1 based on the preparation method of the corps nutrient thing of hydrolysis yeast rich in selenium, it is characterized in that, in described step C, described fermentation substrate being placed in temperature is 37 DEG C, and humidity is the condition bottom fermentation 48h of 85%, obtains yeast rich in selenium mixture.
8. as claimed in claim 3 based on the preparation method of the corps nutrient thing of hydrolysis yeast rich in selenium, it is characterized in that, described pig gastric mucosa collects the fresh pig gastric mucosa in slaughterhouse, by after its packing in-30 DEG C of freezings, during use, described pig gastric mucosa is placed in container, places at 4-7 DEG C, after thawing completely, use after described pig gastric mucosa is ground to form pasty state together with the remaining juice in container.
9. one kind as arbitrary in claim 1-8 as described in based on hydrolysis yeast rich in selenium corps nutrient thing preparation method produce corps nutrient thing.
10. an application for corps nutrient thing as claimed in claim 9, is characterized in that, described yeast rich in selenium base crop nutrient solution, its through dilution 200-300 doubly after for foliage-spray; Described yeast rich in selenium base corps nutrient agent and described yeast rich in selenium base corps nutrient material, it is directly used in soil or crop cultivation matrix, and in soil, consumption is 30-80 kg/acre, and in crop cultivation matrix, addition is 0.5-5%.
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