CN105272412B - Crops Organic selenium nutrient solution and the preparation method and application thereof - Google Patents
Crops Organic selenium nutrient solution and the preparation method and application thereof Download PDFInfo
- Publication number
- CN105272412B CN105272412B CN201510696316.3A CN201510696316A CN105272412B CN 105272412 B CN105272412 B CN 105272412B CN 201510696316 A CN201510696316 A CN 201510696316A CN 105272412 B CN105272412 B CN 105272412B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- weight
- parts
- organic selenium
- nutrient solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation methods of crops Organic selenium nutrient solution, this method carries out fermentation process using bacillus subtilis to Se-enriched yeast, Se-enriched yeast is hydrolyzed using the protease generated in bacillus subtilis metabolic process, selenium-rich hydrolysate is obtained, and then the crops Organic selenium nutrient solution is made；The crops Organic selenium nutrient solution and a kind of application of crops Organic selenium nutrient solution that a kind of preparation method using crops Organic selenium nutrient solution produces.The preparation method of the present invention makes all raw materials obtain abundant, rational utilization, realizes " eating dry squeeze only ", does not occur any residue, raffinate, without any environmental pollution；Crops Organic selenium nutrient solution produced by the present invention, organic selenium content is high, and exists in the form of seleno-amino acids and small peptide, is conducive to the absorption of plant, is remarkably improved the content of Organic Selenium in agricultural product.
Description
Technical field
The present invention relates to organic selenium fertilizer fields.It is more particularly related to a kind of crops Organic selenium nutrient solution and
Preparation method and application.
Background technology
Lack selenium element in the soil in 60% or more China, therefore the universal selenium content of agricultural product is insufficient.According to China 2000
What year formulated《Dietary Reference Intakes of Chinese Residents》, the daily selenium intake of 18 years old or more adult should be 50 micrograms
More than (50 μ g/d), the average daily intake that the World Health Organization (WHO) is recommended is 50~200 μ g/d, but China resident is practical puts down
Equal intake is only 27 micrograms.Some regional extreme selenium deficiency of China, situation are even more serious.Selenium plays anti-cancer, anti-oxidant in human body
Selenium content with the important function for improving immunocompetence, therefore promotion agricultural products in China is the important measures for improving people's health.
Crops are mainly by spraying the blade fertilizer containing sodium selenite, to increase selenium element content in agricultural product at present.
In feed industry, mainly by adding sodium selenite, increase the selenium element content in meat, egg.But to human body it is beneficial that having
Machine selenium (active ingredient is selenomethionine, selenocystine, selenocysteine etc.), inorganic selenium (sodium selenate and sodium selenite)
Instead can harmful to human health.There is stringent limit standard in country to inorganic Se content in agricultural product, as inorganic in cereals
Se content is no more than 0.3mg/kg.The absorbent Organic Selenium of crop leaf is seleno-amino acids (selenomethionine, seleno Guang ammonia
Acid, selenocysteine etc.), and existing Organic Selenium crop leaf spray product cannot be guaranteed selenium element with seleno-amino acids
Form exist.It is therefore desirable to develop the novel crops nutrients for being rich in Organic Selenium, to promote the Organic Selenium in agricultural product
Content.
Invention content
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of crops Organic selenium nutrient solution, Se content is high in the nutrient solution, and
Exist in the form of seleno-amino acids, is conducive to the absorption of crop, is remarkably improved the content of Organic Selenium in agricultural product.
It is a still further object of the present invention to provide a kind of preparation method of crops Organic selenium nutrient solution, this method is using withered
Careless bacillus carries out fermentation process to Se-enriched yeast, using the protease generated in bacillus subtilis metabolic process to selenium-rich
Yeast is hydrolyzed, and obtains selenium-rich hydrolysate, and then the crops Organic selenium nutrient solution is made.
In order to realize these purposes and other advantages according to the present invention, a kind of crops Organic selenium nutrient solution is provided
Preparation method carries out fermentation process to Se-enriched yeast using bacillus subtilis, obtains selenium-rich hydrolysate, the fermentation process includes
Following steps：
Step 1: by the tapioca of the molasses of 3-5 parts by weight, the dregs of beans of 3-5 parts by weight and 5-6 parts by weight in 110-
35min is stirred at 130 DEG C to sterilize, and after sterilizing, is added into the Se-enriched yeast, under the rotating speed of 100-200r/min
1h is stirred, the second fermentation substrate is obtained；
Step 2: it is 20- to spray a small amount of deionized water to the second fermentation substrate water content into second fermentation substrate
40%, the bacillus subtilis of 1-2 parts by weight is added, after stirring 1h under the rotating speed of 100-200r/min, continues to described the
Air washer in two fermentation substrates makes its water content reach 50-70%, then second fermentation substrate is placed in temperature and is
35-40 DEG C, humidity is the 40-56h that ferments under conditions of 85-90%, is persistently stirred with 150r/min in fermentation process, fermentation ends
Afterwards, second fermentation substrate 30min is stirred at 110-130 DEG C to sterilize.
Preferably, the preparation method of the crops Organic selenium nutrient solution, the Se-enriched yeast, by following steps
It is prepared：
Step A, by the molasses of 5-8 parts by weight, the urea of 4-6 parts by weight, the dregs of beans of 6-10 parts by weight and 8-10 parts by weight
Tapioca stir 35min at 110-130 DEG C and sterilize, the temperature of above-mentioned raw materials after sterilization is down to 35 DEG C or less
When, the magnesium chloride of the sodium selenite of 0.05-0.3 parts by weight, the potassium phosphate and 0.5-1.5 parts by weight of 1-3 parts by weight is added, in
1h is stirred under the rotating speed of 100-200r/min, obtains the first fermentation substrate；
Step B, it is 20- that a small amount of deionized water to the first fermentation substrate water content is sprayed into first fermentation substrate
40%, the brewer's yeast of 2-3 parts by weight is added, after stirring 1h under the rotating speed of 100-200r/min, continues to first hair
Air washer in ferment substrate makes its water content reach 50-70%, then it is 35-40 that first fermentation substrate, which is placed in temperature,
DEG C, humidity is the 40-56h that ferments under conditions of 85-90%, is persistently stirred with 150r/min in fermentation process, after fermentation, will
First fermentation substrate stirs 30min at 110-130 DEG C and sterilizes, and obtains Se-enriched yeast.
Preferably, the preparation method of the crops Organic selenium nutrient solution, the fermentation process further include fermenting
Before, the Se-enriched yeast is subjected to Microwave Pretreatment, microwave condition is：Frequency 300-500MHz, time 30-45s.
Preferably, the preparation method of the crops Organic selenium nutrient solution, the fermentation process further include, in institute
It states and uses pressure assisted fermentation in the fermentation process of the second fermentation substrate, wherein when fermentation 18-22h, pressure 0.13-
0.14MPa, remaining fermentation time ferment for normal pressure.
Preferably, the preparation method of the crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution,
For the pure water of 80-120 parts by weight is added into the selenium-rich hydrolysate, 6-8h is stirred under the rotating speed of 150r/min, is passed through
Filter obtained filtrate.
Preferably, in the step 2, described second is sent out for the preparation method of the crops Organic selenium nutrient solution
It is 37 DEG C that ferment substrate, which is placed in temperature, and humidity is fermented 48h under conditions of being 85%, is persistently stirred with 150r/min in fermentation process.
Preferably, the preparation method of the crops Organic selenium nutrient solution, in the step A, the molasses are 5 weights
Part is measured, the urea is 5 parts by weight, and the dregs of beans is 6 parts by weight, and the tapioca is 8 parts by weight, and the sodium selenite is
0.1-0.2 parts by weight, the potassium phosphate are 2 parts by weight, and the magnesium chloride is 1 parts by weight.
A kind of crops Organic selenium nutrient solution of the preparation method production of crops Organic selenium nutrient solution.
A kind of application of crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution are used for leaf after dilution
Face sprays or root application.
The present invention includes at least following advantageous effect：
The first, fermentation process is carried out to Se-enriched yeast using bacillus subtilis, bacillus subtilis generation can be made full use of
It thanks to the protease generated in the process, Se-enriched yeast is made to hydrolyze, ensure that the Organic Selenium in product with seleno-amino acids (seleno egg
Propylhomoserin, selenocystine, selenocysteine etc.) and small peptide form exist, be conducive to the absorption of plant, be remarkably improved agriculture
The content of Organic Selenium in product, and under same hydrolysis degree, compared with using protease, selenium-rich is hydrolyzed using bacillus subtilis
Yeast is at low cost；
The second, before fermentation of bacillus subtilis, by carrying out Microwave Pretreatment to Se-enriched yeast, Se-enriched yeast albumen can be made
Slight cracking, promotes in fermentation process, and Se-enriched yeast hydrolysis is more thorough, improves the content of seleno-amino acids in selenium-rich hydrolysate；
Third carries out pressurized treatments in fermentation of bacillus subtilis 18-22h, at this point, bacillus subtilis is given birth to by logarithm
Enter stationary phase for a long time, pressurized treatments can stimulate bacillus subtilis to be metabolized, and so that it is generated more highly active protein enzymes, promote
Se-enriched yeast is rapidly hydrolyzed into selenium-rich amino acid and small peptide, improves the hydrolysis efficiency and degree of hydrolysis of Se-enriched yeast；
4th, the inventors discovered that, exist in Se-enriched yeast incubation, between sulphur and selenium and vie each other, sulphur is deposited
Absorption of the yeast to selenium element can be being influenced, the organic selenium content in yeast is reduced；It is not used in Se-enriched yeast incubation
The raw material and water of sulfur-bearing, are conducive to yeast and are fully absorbed to selenium element, improve the content of Organic Selenium in yeast；In withered grass gemma
During bacillus fermentation, the raw material and water of sulfur-bearing are not used, can avoid in fermentation process, substitution of the element sulphur to selenium element, drop
Organic selenium content in low selenium-rich hydrolysate；
5th, using the crops Organic selenium nutrient solution for preparing of the present invention, inorganic selenium is not only overcome in agricultural product to people
The harm of body also overcomes the fertilizer damages phenomenon such as burn seedlings, tikka because caused by inorganic selenium fertilizer dosage is improper；
6th, preparation method of the invention makes all raw materials obtain fully, reasonably utilizing, selenium-rich hydrolysate warp
After hybrid filtering, gained filtrate is crops Organic selenium nutrient solution, and gained filter residue can be used as selenium-enriched fertilizer, realize and " eat dry squeezing
Only ", do not occur any residue, raffinate, without any environmental pollution.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail, to enable those skilled in the art with reference to specification
Word can be implemented according to this.
It should be noted that experimental method described in following embodiments is unless otherwise specified conventional method, it is described
Reagent and material commercially obtain unless otherwise specified.
Embodiment 1：
A kind of preparation method of crops Organic selenium nutrient solution carries out at fermentation Se-enriched yeast using bacillus subtilis
Reason, obtains selenium-rich hydrolysate, the fermentation process includes the following steps：
Step 1: the tapioca of the molasses of 3 parts by weight, the dregs of beans of 3 parts by weight and 5 parts by weight is stirred at 110 DEG C
35min sterilizes, and after sterilizing, is added into the Se-enriched yeast, 1h is stirred under the rotating speed of 100r/min, obtain the second hair
Ferment substrate；
Step 2: spraying a small amount of deionized water to the second fermentation substrate water content into second fermentation substrate and being
20%, the bacillus subtilis of 1 parts by weight is added, after stirring 1h under the rotating speed of 100r/min, continues to second fermentation
Air washer in substrate makes its water content up to 50%, then it is 35 DEG C that second fermentation substrate, which is placed in temperature, and humidity is
Ferment 56h under conditions of 85%, is persistently stirred with 150r/min in fermentation process, after fermentation, by second fermentation bottom
Object stirs 30min at 110 DEG C and sterilizes.
In above-mentioned raw materials, molasses can use sucrose or glucose to substitute, and dregs of beans is substituted with other dregs of fat, and tapioca can use it
He substitutes starch, and bacillus subtilis can be substituted with the bacterium or fungi of other production protease.
The preparation method of the crops Organic selenium nutrient solution, the Se-enriched yeast are prepared by following steps：
Step A, by the molasses of 5 parts by weight, the tapioca of the urea of 4 parts by weight, the dregs of beans of 6 parts by weight and 8 parts by weight
35min is stirred at 110 DEG C to sterilize, and when the temperature of above-mentioned raw materials after sterilization is down to 35 DEG C or less, 0.05 weight is added
The magnesium chloride for measuring the sodium selenite of part, the potassium phosphate and 0.5 parts by weight of 1 parts by weight, stirs 1h under the rotating speed of 100r/min, obtains
First fermentation substrate；
Step B, it is 20% that a small amount of deionized water to the first fermentation substrate water content is sprayed into first fermentation substrate,
The brewer's yeast of 2 parts by weight is added, after stirring 1h under the rotating speed of 100r/min, continues to spray into first fermentation substrate
Deionized water makes its water content up to 50%, then it is 35 DEG C that first fermentation substrate, which is placed in temperature, the condition that humidity is 85%
Lower fermentation 56h is persistently stirred in fermentation process with 150r/min, after fermentation, by first fermentation substrate at 110 DEG C
Stirring 30min sterilizes, and obtains Se-enriched yeast.
In above-mentioned raw materials, molasses can use sucrose or glucose to substitute, and dregs of beans is substituted with other dregs of fat, and tapioca can use it
He substitutes starch, and potassium phosphate is substituted with sodium phosphate+potassium chloride, and sodium selenite is substituted with sodium selenate.
The preparation method of the crops Organic selenium nutrient solution, the fermentation process further include, will be described before fermentation
Se-enriched yeast carries out Microwave Pretreatment, and microwave condition is：Frequency 300MHz, time 30s.
The preparation method of the crops Organic selenium nutrient solution, the fermentation process further include, in second fermentation
Pressure assisted fermentation is used in the fermentation process of substrate, wherein when fermentation 18-22h, pressure 0.13MPa, remaining fermentation time
It ferments for normal pressure.
The preparation method of the crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution are to described
The pure water of 80 parts by weight is added in selenium-rich hydrolysate, 6h is stirred under the rotating speed of 150r/min, the filtrate through being obtained by filtration, institute
It obtains filter residue and can be used as selenium-enriched fertilizer.
A kind of crops Organic selenium nutrient solution of the preparation method production of crops Organic selenium nutrient solution.
A kind of application of crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution, organic selenium content are
0.25g/L is added in soil before peanut seeding, and additive amount is 30 kilograms per acre, and organic selenium content is detected after harvesting, is reached
0.3mg/kg is 200-300 times higher than what is do not applied.
Embodiment 2：
A kind of preparation method of crops Organic selenium nutrient solution carries out at fermentation Se-enriched yeast using bacillus subtilis
Reason, obtains selenium-rich hydrolysate, the fermentation process includes the following steps：
Step 1: the tapioca of the molasses of 4 parts by weight, the dregs of beans of 4 parts by weight and 5.5 parts by weight is stirred at 120 DEG C
It mixes 35min to sterilize, after sterilizing, is added into the Se-enriched yeast, stirs 1h under the rotating speed of 150r/min, obtain second
Fermentation substrate；
Step 2: spraying a small amount of deionized water to the second fermentation substrate water content into second fermentation substrate and being
30%, the bacillus subtilis of 1.5 parts by weight is added, after stirring 1h under the rotating speed of 150r/min, continues to second hair
Air washer in ferment substrate makes its water content up to 60%, then it is 37 DEG C that second fermentation substrate, which is placed in temperature, humidity
Ferment 48h under conditions of being 85%, is persistently stirred with 150r/min in fermentation process, and after fermentation, described second is fermented
Substrate stirs 30min at 120 DEG C and sterilizes.
In above-mentioned raw materials, molasses can use sucrose or glucose to substitute, and dregs of beans is substituted with other dregs of fat, and tapioca can use it
He substitutes starch, and bacillus subtilis can be substituted with the bacterium or fungi of other production protease.
The preparation method of the crops Organic selenium nutrient solution, the Se-enriched yeast are prepared by following steps：
Step A, by the molasses of 5 parts by weight, the tapioca of the urea of 5 parts by weight, the dregs of beans of 6 parts by weight and 8 parts by weight
35min is stirred at 120 DEG C to sterilize, and when the temperature of above-mentioned raw materials after sterilization is down to 35 DEG C or less, 0.15 weight is added
Measure the sodium selenite of part, 2 parts by weight potassium phosphate and 1 parts by weight magnesium chloride, stir 1h under the rotating speed of 150r/min, obtain the
One fermentation substrate；
Step B, it is 30% that a small amount of deionized water to the first fermentation substrate water content is sprayed into first fermentation substrate,
The brewer's yeast of 2.5 parts by weight is added, after stirring 1h under the rotating speed of 150r/min, continues to spray into first fermentation substrate
Deionized water is drenched, makes its water content up to 60%, then it is 37 DEG C that first fermentation substrate, which is placed in temperature, the item that humidity is 85%
Ferment 48h under part, is persistently stirred with 150r/min in fermentation process, after fermentation, by first fermentation substrate in 120 DEG C
Lower stirring 30min sterilizes, and obtains Se-enriched yeast.
In above-mentioned raw materials, molasses can use sucrose or glucose to substitute, and dregs of beans is substituted with other dregs of fat, and tapioca can use it
He substitutes starch, and potassium phosphate is substituted with sodium phosphate+potassium chloride, and sodium selenite is substituted with sodium selenate.
The preparation method of the crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution are to described
The pure water of 100 parts by weight is added in selenium-rich hydrolysate, 7h is stirred under the rotating speed of 150r/min, the filtrate through being obtained by filtration,
Gained filter residue can be used as selenium-enriched fertilizer.
A kind of crops Organic selenium nutrient solution of the preparation method production of crops Organic selenium nutrient solution.
A kind of application of crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution, organic selenium content are
0.28g/L is sprayed on oyster mushroom, Pleurotus eryngii bacterium, oyster mushroom bacterium, chicken leg mushroom, often after being diluted 200 times in harvesting the last week
It is primary, continuous three days, and harvesting detects organic selenium content after 3 days, 28-32 μ g/kg (dry weight) is reached, than the high 110- not applied
220 times.
Embodiment 3：
A kind of preparation method of crops Organic selenium nutrient solution carries out at fermentation Se-enriched yeast using bacillus subtilis
Reason, obtains selenium-rich hydrolysate, the fermentation process includes the following steps：
Step 1: the tapioca of the molasses of 5 parts by weight, the dregs of beans of 5 parts by weight and 6 parts by weight is stirred at 130 DEG C
35min sterilizes, and after sterilizing, is added into the Se-enriched yeast, 1h is stirred under the rotating speed of 200r/min, obtain the second hair
Ferment substrate；
Step 2: spraying a small amount of deionized water to the second fermentation substrate water content into second fermentation substrate and being
40%, the bacillus subtilis of 2 parts by weight is added, after stirring 1h under the rotating speed of 200r/min, continues to second fermentation
Air washer in substrate makes its water content up to 70%, then it is 40 DEG C that second fermentation substrate, which is placed in temperature, and humidity is
Ferment 40h under conditions of 90%, is persistently stirred with 150r/min in fermentation process, after fermentation, by second fermentation bottom
Object stirs 30min at 130 DEG C and sterilizes.
In above-mentioned raw materials, molasses can use sucrose or glucose to substitute, and dregs of beans is substituted with other dregs of fat, and tapioca can use it
He substitutes starch, and bacillus subtilis can be substituted with the bacterium or fungi of other production protease.
The preparation method of the crops Organic selenium nutrient solution, the Se-enriched yeast are prepared by following steps：
Step A, by the molasses of 8 parts by weight, the para arrowroot of the urea of 6 parts by weight, the dregs of beans of 10 parts by weight and 10 parts by weight
Powder stirs 35min at 130 DEG C and sterilizes, and when the temperature of above-mentioned raw materials after sterilization is down to 35 DEG C or less, 0.3 weight is added
The magnesium chloride for measuring the sodium selenite of part, the potassium phosphate and 1.5 parts by weight of 3 parts by weight, stirs 1h under the rotating speed of 200r/min, obtains
First fermentation substrate；
Step B, it is 40% that a small amount of deionized water to the first fermentation substrate water content is sprayed into first fermentation substrate,
The brewer's yeast of 3 parts by weight is added, after stirring 1h under the rotating speed of 200r/min, continues to spray into first fermentation substrate
Deionized water makes its water content up to 70%, then it is 40 DEG C that first fermentation substrate, which is placed in temperature, the condition that humidity is 90%
Lower fermentation 40h is persistently stirred in fermentation process with 150r/min, after fermentation, by first fermentation substrate at 130 DEG C
Stirring 30min sterilizes, and obtains Se-enriched yeast.
In above-mentioned raw materials, molasses can use sucrose or glucose to substitute, and dregs of beans is substituted with other dregs of fat, and tapioca can use it
He substitutes starch, and potassium phosphate is substituted with sodium phosphate+potassium chloride, and sodium selenite is substituted with sodium selenate.
The preparation method of the crops Organic selenium nutrient solution, the fermentation process further include, will be described before fermentation
Se-enriched yeast carries out Microwave Pretreatment, and microwave condition is：Frequency 500MHz, time 45s.
The preparation method of the crops Organic selenium nutrient solution, the fermentation process further include, in second fermentation
Pressure assisted fermentation is used in the fermentation process of substrate, wherein when fermentation 18-22h, pressure 0.14MPa, remaining fermentation time
It ferments for normal pressure.
The preparation method of the crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution are to described
The pure water of 120 parts by weight is added in selenium-rich hydrolysate, 8h is stirred under the rotating speed of 150r/min, the filtrate through being obtained by filtration,
Gained filter residue can be used as selenium-enriched fertilizer.
A kind of crops Organic selenium nutrient solution of the preparation method production of crops Organic selenium nutrient solution.
A kind of application of crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution, organic selenium content are
0.40g/L starts to spray after being diluted 400 times in rice earing flowering initial stage, is sprayed every 5 days once, altogether three times, harvesting
After detect organic selenium content, reach 0.29mg/kg, it is 200-250 times higher than what is do not applied.
Embodiment 4：
On the basis of embodiment 3, the fermentation process further includes, and before fermentation, it is pre- that the Se-enriched yeast is carried out microwave
Processing, microwave condition are：Frequency:400MHz, time 35s；Pressure assisted fermentation is used in fermentation process, wherein fermentation 18-
When 22h, pressure 0.135MPa, remaining fermentation time ferments for normal pressure.
A kind of application of crops Organic selenium nutrient solution, the crops Organic selenium nutrient solution, organic selenium content are
0.36g/L is sprayed on oyster mushroom, Pleurotus eryngii bacterium, oyster mushroom bacterium, chicken leg mushroom, often after being diluted 200 times in harvesting the last week
It is primary, continuous three days, and harvesting detects organic selenium content after 3 days, 30-35 μ g/kg (dry weight) is reached, than the high 120- not applied
230 times.
Comparative example 1：
The middle raw material culture yeast rich in selenium using not sulfur-bearing is further illustrated the present invention by comparative example 1 prepares farming
The advantageous effect of object Organic selenium nutrient solution.Wherein, control group is to replace with the potassium phosphate of 2 parts by weight in 2 step A of embodiment
The potassium sulfate of 2 parts by weight, composition, proportioning and the operating condition of remaining raw material of control group are the same as embodiment 2.Experimental result see the table below.
| Organic selenium content (g/L) in crops Organic selenium nutrient solution | |
| Control group | 0.12 |
| Embodiment 2 | 0.28 |
As seen from the above table, in control group, the organic selenium content in the crops Organic selenium nutrient solution of gained is substantially less than
Embodiment 2 illustrates that the presence of the sulfate ion in potassium sulfate can significantly affect brewer's yeast pair in Se-enriched yeast incubation
The absorption of selenium causes the organic selenium content in yeast to reduce, and then makes the Organic Selenium in crops Organic selenium nutrient solution obtained
Content reduces；The raw material and water that sulfur-bearing is not used in Se-enriched yeast incubation, are conducive to substitution and selenium element of the selenium to sulphur
Fully absorb, improve Se-enriched yeast in Organic Selenium content.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (8)
1. a kind of preparation method of crops Organic selenium nutrient solution, which is characterized in that using bacillus subtilis to Se-enriched yeast
Fermentation process is carried out, obtains selenium-rich hydrolysate, the fermentation process includes the following steps：
Step 1: by the tapioca of the molasses of 3-5 parts by weight, the dregs of beans of 3-5 parts by weight and 5-6 parts by weight in 110-130 DEG C
Lower stirring 35min sterilizes, and after sterilizing, is added into the Se-enriched yeast, is stirred under the rotating speed of 100-200r/min
1h obtains the second fermentation substrate；
Step 2: it is 20-40% that a small amount of deionized water to the second fermentation substrate water content is sprayed into second fermentation substrate,
The bacillus subtilis of 1-2 parts by weight is added, after stirring 1h under the rotating speed of 100-200r/min, continues to second fermentation
Air washer in substrate makes its water content reach 50-70%, then it is 35-40 DEG C that second fermentation substrate, which is placed in temperature,
Humidity is the 40-56h that ferments under conditions of 85-90%, is persistently stirred with 150r/min in fermentation process, after fermentation, by institute
It states the second fermentation substrate and stirs 30min at 110-130 DEG C and sterilize；
The Se-enriched yeast, is prepared by following steps：
Step A, by the molasses of 5-8 parts by weight, the wood of the urea of 4-6 parts by weight, the dregs of beans of 6-10 parts by weight and 8-10 parts by weight
Sweet potato starch stirs 35min at 110-130 DEG C and sterilizes, when the temperature of above-mentioned raw materials after sterilization is down to 35 DEG C or less,
The magnesium chloride of the sodium selenite of 0.05-0.3 parts by weight, the potassium phosphate and 0.5-1.5 parts by weight of 1-3 parts by weight is added, in 100-
1h is stirred under the rotating speed of 200r/min, obtains the first fermentation substrate；
Step B, it is 20-40% that a small amount of deionized water to the first fermentation substrate water content is sprayed into first fermentation substrate,
The brewer's yeast of 2-3 parts by weight is added, after stirring 1h under the rotating speed of 100-200r/min, continues to first fermentation substrate
Middle Air washer makes its water content reach 50-70%, then it is 35-40 DEG C that first fermentation substrate, which is placed in temperature, humidity
For the 40-56h that ferments under conditions of 85-90%, persistently stirred with 150r/min in fermentation process, after fermentation, by described
One fermentation substrate stirs 30min at 110-130 DEG C and sterilizes, and obtains Se-enriched yeast.
2. the preparation method of crops Organic selenium nutrient solution as described in claim 1, which is characterized in that the fermentation process,
Further include before fermentation, the Se-enriched yeast being subjected to Microwave Pretreatment, microwave condition is：Frequency 300-500MHz, time 30-
45s。
3. the preparation method of crops Organic selenium nutrient solution as described in claim 1, which is characterized in that the fermentation process,
Further include that pressure assisted fermentation is used in the fermentation process of second fermentation substrate, wherein when fermentation 18-22h, pressure
For 0.13-0.14MPa, remaining fermentation time ferments for normal pressure.
4. the preparation method of crops Organic selenium nutrient solution as described in claim 1, which is characterized in that the crops are organic
Selenium nutrient solution stirs for the pure water of 80-120 parts by weight is added into the selenium-rich hydrolysate under the rotating speed of 150r/min
6-8h is mixed, the filtrate through being obtained by filtration.
5. the preparation method of crops Organic selenium nutrient solution as described in claim 1, which is characterized in that in the step 2,
It is 37 DEG C that second fermentation substrate, which is placed in temperature, and humidity is fermented 48h under conditions of being 85%, with 150r/ in fermentation process
Min is persistently stirred.
6. the preparation method of crops Organic selenium nutrient solution as described in claim 1, which is characterized in that in the step A, institute
It is 5 parts by weight to state molasses, and the urea is 5 parts by weight, and the dregs of beans is 6 parts by weight, and the tapioca is 8 parts by weight, institute
It is 0.1-0.2 parts by weight to state sodium selenite, and the potassium phosphate is 2 parts by weight, and the magnesium chloride is 1 parts by weight.
7. a kind of crops of the preparation method production of crops Organic selenium nutrient solution as described in claim 1-6 is any are organic
Selenium nutrient solution.
8. a kind of application of crops Organic selenium nutrient solution as claimed in claim 7, which is characterized in that the crops are organic
Selenium nutrient solution is used for foliage-spray or root application after dilution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510696316.3A CN105272412B (en) | 2015-10-23 | 2015-10-23 | Crops Organic selenium nutrient solution and the preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510696316.3A CN105272412B (en) | 2015-10-23 | 2015-10-23 | Crops Organic selenium nutrient solution and the preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105272412A CN105272412A (en) | 2016-01-27 |
| CN105272412B true CN105272412B (en) | 2018-11-02 |
Family
ID=55142327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510696316.3A Active CN105272412B (en) | 2015-10-23 | 2015-10-23 | Crops Organic selenium nutrient solution and the preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105272412B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105294254A (en) * | 2015-10-29 | 2016-02-03 | 广西锦翰环保科技有限公司 | Crop nutrient based on hydrolyzed selenium-enriched yeast and preparation method and application of crop nutrient |
| CN105859356A (en) * | 2016-04-22 | 2016-08-17 | 长沙学院 | Special plant nutrient for producing polypeptide vegetables and preparation method thereof |
| CN106431571A (en) * | 2016-09-30 | 2017-02-22 | 桂林淮安天然保健品开发有限公司 | Ganoderma lucidum karst cultivation method for improving ganoderma lucidum polysaccharides and selenium content |
| CN106478236A (en) * | 2016-09-30 | 2017-03-08 | 桂林淮安天然保健品开发有限公司 | The Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved |
| CN107056503A (en) * | 2017-06-08 | 2017-08-18 | 合肥慧明瀚生态农业科技有限公司 | It is a kind of for nutrient solution of soilless culture and preparation method thereof |
| CN107216203A (en) * | 2017-06-28 | 2017-09-29 | 太仓市绿苗农场专业合作社 | A kind of selenium-rich water planting growth-promoting media |
| CN107396795A (en) * | 2017-07-27 | 2017-11-28 | 杨喜弟 | A kind of implantation methods for improving crisp pears quality |
| CN107801581A (en) * | 2017-10-16 | 2018-03-16 | 茂名华绿农牧科技有限公司 | A kind of implantation methods of selenium-rich leaf mustard |
| CN110317100A (en) * | 2019-07-22 | 2019-10-11 | 唐山津国农业技术开发有限公司 | The production technology of organic selenium Liquid Fertilizer |
| CN113197075B (en) * | 2021-03-11 | 2023-11-24 | 湖北国硒科技发展有限公司 | Processing method of asparagus rich in organic selenium |
| CN115477554A (en) * | 2022-10-09 | 2022-12-16 | 广东博沃特生物技术有限公司 | Selenium-enriched foliar fertilizer and preparation method and application thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113950A (en) * | 1994-05-13 | 1995-12-27 | 刘力 | Selenium enriched yeast and its producing technology |
| FR2904184B1 (en) * | 2006-07-28 | 2008-10-03 | Innovation Tech Expansion Comm | PROCESS FOR TREATING CROP PLANTS TO ENRICH IN SELENIUM AND USE OF PLANTS THUS PROCESSED |
| CN103145488B (en) * | 2012-11-28 | 2014-05-21 | 梁树森 | Multiple microbial manure applied in summer to increase quality of black tea and preparation method thereof |
| CN104108962A (en) * | 2014-07-03 | 2014-10-22 | 姚海斌 | Method for preparing organic selenium fertilizer through hydrolysis of selenium-enriched yeast |
| CN104276892B (en) * | 2014-10-13 | 2016-09-07 | 湖南省林业科学院 | A kind of Caulis Bambusae Germinatus special selenium-enriched liquid fertilizer and preparation method thereof |
| CN104557340A (en) * | 2015-02-09 | 2015-04-29 | 刘备 | Special microbial organic fertilizer for selenium-enriched rice |
-
2015
- 2015-10-23 CN CN201510696316.3A patent/CN105272412B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105272412A (en) | 2016-01-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105272412B (en) | Crops Organic selenium nutrient solution and the preparation method and application thereof | |
| CN104446813A (en) | Bioorganic fertilizer and preparation method thereof | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN104222668A (en) | Compound fish feed capable of improving body immunity | |
| CN104351901A (en) | Probiotics fermented chlorella beverage and production method thereof | |
| CN105110841B (en) | Culture base-material using Lenlinus edodes slag for cultivating hickory chick and preparation method thereof | |
| CN104017739B (en) | Complex micro organism fungicide, its preparation method and its application in high protein potato dreg fodder is produced | |
| CN103387463A (en) | Cultivation method and cultivation culture material of black fungus | |
| CN103319271A (en) | Indoor practical straw mushroom medium and its preparation method | |
| CN109748607A (en) | A kind of dedicated Selenium-rich nutrient solution of greenhouse crops and preparation method thereof | |
| CN106387336A (en) | Corn slurry fermented feed and production method thereof | |
| CN103922851A (en) | Black fungus liquid medium and black fungus cultural method | |
| CN104172137A (en) | Production process of selenium-rich edible fungus powder | |
| CN107360858A (en) | A kind of breeding method of mushroom edible mushroom | |
| CN112174724A (en) | Marine biological fertilizer capable of improving crop immunity | |
| CN109511461A (en) | A kind of high yield cultivating in bag method of black fungus | |
| CN104261980A (en) | Bio-organic fertilizer for planting lotus root | |
| CN110447456A (en) | A kind of selenium-enriched edible mushroom production technology | |
| CN102487725A (en) | Method for culturing hypsizygus marmoreus by using corn byproduct | |
| CN110122657A (en) | A kind of herbage biological feedstuff leavening | |
| CN104557154A (en) | Method for producing amino acid fertilizer from soybean meal through solid-state fermentation | |
| CN104206172A (en) | Cultivation method for selenium-enriched oyster mushrooms with small mushroom caps and large stipes | |
| CN109287853B (en) | Corn straw-soybean meal mixed fermentation material, and preparation method and application thereof | |
| CN101756011A (en) | Method for utilizing dual-strains solid fermented sauce/vinegar dregs to produce multi-enzyme high-lysine feedstuff | |
| CN105294254A (en) | Crop nutrient based on hydrolyzed selenium-enriched yeast and preparation method and application of crop nutrient |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |