CN105284404B - The method that Arbuscular Mycorrhizal Fungi promotes hemp growth - Google Patents
The method that Arbuscular Mycorrhizal Fungi promotes hemp growth Download PDFInfo
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- CN105284404B CN105284404B CN201510850010.9A CN201510850010A CN105284404B CN 105284404 B CN105284404 B CN 105284404B CN 201510850010 A CN201510850010 A CN 201510850010A CN 105284404 B CN105284404 B CN 105284404B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The present invention relates to a kind of methods that Arbuscular Mycorrhizal Fungi promotes hemp growth, and this method handles by seed collection, sprout and be inoculated with, and determination step completion is observed and infected to growing state, through the experimental results showed that：Glomus mosseae Glomus mosseaeIt is inoculated with hempPoacynum pictum, to hempPoacynum pictumThe average plant height of plant, average growth rate, clump width, four important growth indexes of the number of blade, which show, to be apparently higher than to two non-seeded control groups and other AM fungal biodegradation groups.Glomus mosseae simultaneouslyGlomus mosseaeTo hempPoacynum pictumThere are high infection rate and high infection strength, is higher than other all processing groups, therefore to hempPoacynum pictumIt is inoculated with Glomus mosseaeGlomus mosseaeGrowth after strain is obviously promoted effect.
Description
Technical field
The present invention relates to a kind of Arbuscular Mycorrhizal Fungis to promote the method that hemp (Poacynum pictum) grows.
Background technology
Mycorhiza (Mycorrhizae) is a kind of universal plant symbiosis phenomenon of nature, and arbuscular mycorrhiza (AM fungies) is then
Distribution is most wide, with Agriculture, forestry And Animal Husbandry relationship a kind of mycorhiza the closest, 90% or more vascular plant can form clump branch on the earth
Mycorhiza.In arid area, the reconstruction of vegetation and the relationship of arbuscular mycorrhiza (AM fungies) are very close, some plants must rely on bacterium
Root community could survive or complete its life cycle；Plant roots and fungi community make a profit number and time and arbuscular mycorrhiza
The range of (AM fungies) group is related, and generally the high level of quick growth of plant phase and fungal colonization is related；Clump branch bacterium
Root (AM fungies) plays important role in Arid land ecosystems, orients and connects in plant introduction sexual reproduction process
Kind of arbuscular mycorrhiza (AM fungies), will help advantitious plant survive, grow, population child care, and can be the extensive of ecosystem function
Theoretical foundation is provided again, it is medicinal to arid biogeographic zone Precious, Rare, Endangered, economic plants to introduce a fine variety breeding meaning great.
There is the arid area of large area in China western part, since the unreasonable production activity of the mankind and fragile ecological environment are mutual
Effect cause Land capability to decline, land resource lose, desertification, desertification threat getting worse.Some are in ecology
The number of plant played an important roll in system reduces or is on the verge of impasse.
Research shows that very close in the reconstruction of arid area vegetation and the relationship of arbuscular mycorrhiza (AM fungies).Some plants
Must could survive or complete by mycorhiza community its life cycle；Other plants have benefited from mycorhiza under certain condition,
It is facultative community；And there are some plants infecting rear output and survival rate decline by arbuscular mycorrhiza (AM fungies).Plant roots
With fungi community make a profit number and the time it is related with arbuscular mycorrhiza (AM fungies) range of group, advantitious plant and clump bacterium
The functional evidence contacted that may be present between root (AM fungies), it is possible to which (AM is true with root arbuscular mycorrhiza by detecting it
Bacterium) pattern of group finds；Habitat characteristics (soil physical chemistry the hydrology weather etc.) or the ecology of arbuscular mycorrhiza (AM fungies) are special
Point may determine the dynamic change of arbuscular mycorrhiza (AM fungies).And plant growth the difference of feature and plant site may
Cause the prodigious difference of arbuscular mycorrhiza (AM fungies) taxon, to which the growth and development of plant can be influenced, because of different clump branches
Mycorhiza (AM fungies) taxon is supplied to the nutrient of plant at them and the ability of plant growth is promoted also to have prodigious difference, especially
It is that generally the high level of quick growth of plant phase and fungal colonization is related by plant very strong to mycorrhizal dependency, with
The type of fungi is also related.The study found that arbuscular mycorrhiza (AM fungies) have the ability by distribution of the nutrition between aulophyte come
The symbiosis of plant is adjusted, a kind of nutritional amt that plant is obtained determines the symbiosis of its competitiveness and it and other plants
Ability.Arbuscular mycorrhiza (AM fungies) type is rich and group's composition is if a kind of plant of different niches has similitude,
So the structure of phytobiocoenose may be subjected to the influence of arbuscular mycorrhiza (AM fungies) type, and in turn, aulophyte may
Also the structure of arbuscular mycorrhiza (AM fungies) group can be influenced, and it is also possible to plant shows with arbuscular mycorrhiza (AM fungies) type
A kind of interdepend adapts to the relationship of these habitat soil and weather conditions.
The effect generated on aulophyte forms such as arbuscular mycorrhiza (AM fungies) type and changes, arbuscular mycorrhiza
(AM fungies) is also incoordinate, arbuscular mycorrhiza (AM fungies) group of one ecosystem of change or part change to the effect of plant
It falls and just influences whether next process, in semiarid Mediterranean Region arbuscular mycorrhiza (AM fungies) population quantity because of agriculture behavior
And reduce, just restore after agro-farming stops 45 years, Stribley (1987) suggests mycorhiza for plant in natural ecosystems
In establiss and survive it is critically important, and emphasize measure an ecosystem in indigenous bacterium kinds of root it is also extremely important.
Therefore, in Arid land ecosystems, arbuscular mycorrhiza (AM fungies) plays important role, is not only the possibility to
The type of plant, the distribution of nutritional resource are decide with together with other biologies and abiotic factor, it could even be possible to influencing plant
Structure of community and diversity.Type, quantity and its life during introducing a fine variety pair with the symbiosis arbuscular mycorrhiza (AM fungies) of plant
State feature is recognized, and introduces a fine variety transplanting plant survival, growth, population child care then will not only help, but also can be ecosystem
The recovery for function of uniting provides theoretical foundation, to implement more effective recovery measure；In arid area, agriculture forest and husbandry produces, is medicinal
Artificial Arbuscular Mycorrhizal Fungi (AM fungies) during the introducing a fine variety of plant in plant exploitation, will promote growth and development, the enhancing of plant
Plant resistance to environment stress improves crop yield and quality, improves medicament effective component.
Invention content
Present invention aims at provide a kind of method that Arbuscular Mycorrhizal Fungi promotes hemp to grow, this method is by planting
Sub- acquisition process, sprout and inoculation, growing state observe and infect determination step completion, through the experimental results showed that：E Moses's sacculus
Mould Glomus mosseae are inoculated with hemp Poacynum pictum, to the average plant height of hemp Poacynum pictum plant,
Average growth rate, clump width, four important growth indexes of the number of blade show be apparently higher than to two non-seeded control groups and
Other AM fungal biodegradation groups.E Glomus mosseae Glomus mosseae have high infection rate to hemp Poacynum pictum simultaneously
With high infection strength, it is higher than other all processing groups, therefore E Glomus mosseaes are inoculated with to hemp Poacynum pictum
Growth after Glomus mosseae strains is obviously promoted effect.
The method that Arbuscular Mycorrhizal Fungi of the present invention promotes hemp growth, this method follow these steps to carry out：
Seed collection processing：
A, the hemp seed for picking up from full health after natural drying, impregnates 15min with NaClO solution, and sterile water washes down,
After seed soaking for 24 hours, it is put into the sterilized culture dish equipped with double-layer filter paper, is handled；
It sprouts and is inoculated with：
B, the hemp seed after step a sterilizings is placed in growth cabinet culture, climate box sets daily day temperature 25
DEG C, it cultivates 14 hours, 14 DEG C of night temperatures, cultivates 10 hours, after the sprouting of hemp seed, length is spare to 1-2cm；
C, experimental field 6 groups of the nutritive cube of 10cm × 15cm, every group of 15 nutritive cubes, then by 6 groups of nutrition are selected in the wild
The sandy soil after conventional method sterilizes of each nutritive cube paving 10cm thickness in alms bowl, distinguish uniform 6 kinds of A childrens of spreading on sandy soil surface
Set sacculus is mould, and the ivory white sacculus of B is mould, and C clumping sacculus is mould, and the closely bright sacculus of D is mould, and E Glomus mosseaes contain during F stratiform sacculus is mould
Then the washed-out sand microbial inoculum of fungal spore diameter 2mm, spreading thickness 2-3mm spread the frequent of 2mm thickness on 6 kinds of microbial inoculum surfaces respectively
Rule method sterilizing sandy soil, then be uniformly dispersed on sandy soil and place 5 plants of the seedling that the spare hemps of step b sprout, it is put down again on seedling
It spreads that 2mm is thick to sterilize sandy soil through conventional method, then waters；
Measurement is observed and infected to growing state：
D, start the plant height to each plant, clump width, Cong Jing, the number of blade, speed of growth data after emergence 10d to carry out periodically
It observes, takes root segment to fix after 90d, each processing takes more than 100 sections, altogether about more than 800 root segments, for infecting observation experiment in next step.
The spore density in fungal spore in step c is 25-100 spore/ml.
Hemp Poacynum pictum (Schrenk) Baill. Apocynaceaes, hemp category, I grade of Xinjiang protection species are
Arid biogeographic zone rare or endangered species species.
Arid biogeographic zone rare or endangered species hemp Poacynum pictum seeds are husky by Chinese Academy of Sciences Turfan in the present invention
Unconcerned botanical garden or its acquisition；Arbuscular mycorrhizal fungi 6 is the ivory white sacculus of A Glomus etunicatum Glomus etunicatum, B
Mould Glomus eburneum, the mould Glomus aggregatum of C clumping sacculus, the closely bright sacculus of D mould Glomus claroideum, E
The mould Glomus lamellosum of Glomus mosseae Glomus mosseae, F stratiform sacculus are sought by Beijing City Agriculture and Forestry Institute plant
It supports and expands with the Study on Resources institute " arbuscular mycorrhizal fungi germplasm resource bank (Bank of Glomeromycota in China, BGC) "
Numerous and offer.
Description of the drawings
Fig. 1 is present invention growth schematic diagram.
Specific implementation mode
As subjects hemp Poacynum pictum seeds, received respectively between 2013-2014 6-8 month fructescences
The seed for collecting full health, picks up from Chinese Academy of Sciences's Turpan Desert Botanical Garden.
Embodiment
Seed collection processing：
A, the hemp Poacynum pictum seeds for picking up from full health after natural drying, are impregnated with NaClO solution
15min, sterile water wash down, and after seed soaking for 24 hours, are put into the sterilized culture dish equipped with double-layer filter paper, are handled；
It sprouts and is inoculated with：
B, the hemp Poacynum pictum seeds merging growth cabinet culture after step a sterilizing, 25 DEG C of temperature,
Culture 14 hours, 14 DEG C of temperature are cultivated 10 hours, and after the sprouting of hemp Poacynum pictum seeds, length is spare to 1-2cm；
C, experimental field 6 groups of the nutritive cube of 10cm × 15cm, every group of 15 nutritive cubes, then by 6 groups of nutrition are selected in the wild
The sandy soil (soil data is shown in Table 1 table 2) after conventional method sterilizes of each nutritive cube paving 10cm thickness in alms bowl, on sandy soil surface
Washed-out sand microbial inoculum containing fungal spore diameter 2mm in uniform 6 kinds of A Glomus etunicatum Glomus etunicatum of spreading respectively,
Washed-out sand microbial inoculum containing fungal spore diameter 2mm in the mould Glomus eburneum of the ivory white sacculus of B, C clumping sacculus are mould
Washed-out sand microbial inoculum containing fungal spore diameter 2mm in Glomus aggregatum, the closely bright mould Glomus of sacculus of D
Washed-out sand microbial inoculum containing fungal spore diameter 2mm in claroideum, containing true in E Glomus mosseae Glomus mosseae
The washed-out sand microbial inoculum of bacterium spore diameter 2mm contains fungal spore diameter 2mm's in the mould Glomus lamellosum of F stratiform sacculus
Washed-out sand microbial inoculum, spreading thickness 2-3mm, then sterilized sandy soil through conventional method 6 kinds of microbial inoculum surfaces paving 2mm are thick respectively, then
It is uniformly dispersed on sandy soil and places 5 plants of the seedling that the spare hemp Poacynum pictum of step b sprout, tile again on seedling
2mm is thick to sterilize sandy soil through conventional method, then waters；It is set simultaneously there are two control, blank control CK1 and quartz sand compare
CK2, blank control CK1 are direct kind of 5 plants of hemp seedling on the sterile sandy soil of preset 10cm, the sterilizing for the 2mm thickness that tiles again on seedling
Sandy soil, 15 repetitions, quartz sand control control CK2 is first tile on the sterile sandy soil of preset 10cm 3mm physicochemical properties and band
There is the identical sterile no spore washing of the Inoculant of spore husky, repaves sandy soil after the sterilizing of 2mm thickness, be uniformly dispersed and put to 5 plants in vain
Numb seedling, the sterilizing sandy soil for the 2mm thickness that tiles again on seedling, 15 repetitions；
D, measurement is observed and infected to growing state
Start the plant height to each plant, clump width, Cong Jing, the number of blade, speed of growth data after emergence 10d periodically to be seen
It surveys, takes root segment to fix after 90d, each each processing takes 80-100 sections, and about more than 800 root segments, real for infecting observation in next step altogether
It tests.
Soil nutrient data：Table 1
| Sample ID | Organic carbon (g/kg) | Full nitrogen (g/kg) | Full phosphorus (g/kg) | Fast nitrogen (mg/kg) | Fast phosphorus (mg/kg) |
| 14.165 | 0.167 | 1.162 | 562.464 | 5.976 |
Soil nutrient data：Table 2
| Sample ID | PH | Cl-(mg/g) | SO4 2-(mg/g) | Ca2+(mg/g) | K+(mg/g) | Mg2+(mg/g) | Na+(mg/g) | CO3 2-(mg/g) |
| 7.92 | 0.15211 | 1.275935 | 0.399442 | 0.046193 | 0.05188 | 0.28248 | 0 |
Hemp (Poacynum pictum) upgrowth situation under 3 difference AM fungal biodegradations of table
Hemp (Poacynum pictum) infects data under 4 difference AM fungal biodegradations of table
It is shown according to the observed result of growth period 151d, the plant after E Glomus mosseae Glomus mosseae processing
Average plant height 35.50 ± 0.50 is showed with average growth rate 0.24 ± 0.003 is apparently higher than control group and other AM fungies
The phenomenon that processing group, clump width, the number of blade have increased resistance invasion also above to two non-seeded control groups and other AM fungal biodegradation groups
Dye rate 93.33 and high infection strength 47.33 show E Glomus mosseae Glomus mosseae to hemp Poacynum pictum
Growth produce apparent facilitation.In contrast, compared with control group and other AM fungal biodegradation groups, F stratiforms
The average plant height 18.78 ± 5.29 of the mould Glomus lamellosum processing groups of sacculus is omited with average growth rate 0.12 ± 0.04
It is low.Remaining 4 A Glomus etunicatum Glomus etunicatum, B mould Glomus eburneum of ivory white sacculus, C clumping sacculus
The mould Glomus claroideum of the closely bright sacculus of mould Glomus aggregatum, D are relative to control group to plant growth condition
It does not make significant difference.Therefore, the hemp Poacynum pictum lifes after E Glomus mosseae Glomus mosseae fungal biodegradations
Long situation, which has, to be significantly improved.
Claims (1)
1. a kind of method that Arbuscular Mycorrhizal Fungi promotes hemp growth, it is characterised in that this method follows these steps to carry out：
Seed collection processing：
A, the hemp seed for picking up from full health after natural drying, impregnates 15 min with NaClO solution, and sterile water washes down, and soaks
After 24 h of kind, it is put into the sterilized culture dish equipped with double-layer filter paper, is handled；
It sprouts and is inoculated with：
B, the hemp seed after step a sterilizings is placed in growth cabinet culture, climate box sets daily 25 DEG C of day temperature, training
It supports 14 hours, 14 DEG C of night temperatures, cultivates 10 hours, after the sprouting of hemp seed, length is spare to 1-2 cm；
C, experimental field 6 groups of the nutritive cube of the cm of 10 cm × 15, every group of 15 nutritive cubes, then by 6 groups of nutritive cubes are selected in the wild
In each nutritive cube paving 10cm thickness the sandy soil after conventional method sterilizes, in the uniform 6 kinds of A childrens of spreading of sandy soil surface difference
Set sacculus is mould, and the ivory white sacculus of B is mould, and C clumping sacculus is mould, and the closely bright sacculus of D is mould, E Glomus mosseaes, and F stratiform sacculus is mould
In the washed-out sand microbial inoculum containing fungal spore diameter 2mm, spreading thickness 2-3mm, then on 6 kinds of microbial inoculum surfaces, paving 2mm are thick respectively
Sterilize sandy soil through conventional method, then be uniformly dispersed on sandy soil and to place 5 plants of the seedling that the spare hemps of step b sprout, seedling
On tile that 2mm is thick to sterilize sandy soil through conventional method again, then water, wherein the spore density in fungal spore is 25-100
A spore/ml；
Measurement is observed and infected to growing state：
D, start the plant height to each plant, clump width, Cong Jing, the number of blade, speed of growth data after emergence 10d periodically to be seen
It surveys, takes root segment to fix after 90d, each processing takes more than 100 sections, altogether about more than 800 root segments, for infecting observation experiment in next step.
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| CN110651558B (en) * | 2019-10-12 | 2021-07-23 | 咸宁市农业科学院 | Method for improving germination rate of ramie seeds |
| CN110862929B (en) * | 2019-12-09 | 2020-11-06 | 江西农业大学 | Gloeostereum incarnatum, microbial inoculum comprising same and application thereof |
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| JPH03236721A (en) * | 1989-12-22 | 1991-10-22 | Kanegafuchi Chem Ind Co Ltd | Method for cultivating blanched welsh onion and tool used therefor |
| CN101306427A (en) * | 2007-05-17 | 2008-11-19 | 中国科学院生态环境研究中心 | Method for increasing organic pollutant degradation efficiency in soil |
| CN102172136A (en) * | 2011-01-25 | 2011-09-07 | 中国科学院新疆生态与地理研究所 | Method for improving artificial breeding survival rate of populus diversifolia by inoculating arbuscular mycorrhizal fungi |
| CN103242083A (en) * | 2013-05-24 | 2013-08-14 | 常熟市润丰农业有限公司 | Microbial organic fertilizer |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100224467B1 (en) * | 1997-10-02 | 1999-10-15 | 차동천 | The turf mat containing mycorrhiza |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03236721A (en) * | 1989-12-22 | 1991-10-22 | Kanegafuchi Chem Ind Co Ltd | Method for cultivating blanched welsh onion and tool used therefor |
| CN101306427A (en) * | 2007-05-17 | 2008-11-19 | 中国科学院生态环境研究中心 | Method for increasing organic pollutant degradation efficiency in soil |
| CN102172136A (en) * | 2011-01-25 | 2011-09-07 | 中国科学院新疆生态与地理研究所 | Method for improving artificial breeding survival rate of populus diversifolia by inoculating arbuscular mycorrhizal fungi |
| CN103242083A (en) * | 2013-05-24 | 2013-08-14 | 常熟市润丰农业有限公司 | Microbial organic fertilizer |
Non-Patent Citations (1)
| Title |
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| 荒漠河岸林植物丛枝菌根真菌侵染及环境影响因子-以塔里木河下游为例;杨玉海等;《自然科学进展》;20080430;第18卷(第4期);第397-405页 * |
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