CN105265927A - Preparation method of water-soluble dietary fiber from soybean dregs by microbial fermentation - Google Patents
Preparation method of water-soluble dietary fiber from soybean dregs by microbial fermentation Download PDFInfo
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Abstract
The invention relates to a preparation method of water-soluble dietary fiber from soybean dregs by microbial fermentation. The preparation method comprises the following steps: mixing soybean dregs with water to obtain mixed liquor; grafting acclimated Rhizopusoryzae Wentet Pr. Geerl. into the mixed liquor to ferment; dispersing the fermented soybean dregs in water and steaming by high temperature and high pressure; separating by centrifuge, so as to obtain fat, supernatant and residue; dispersing the residue in water then performing second steaming, separating by centrifuge and combining the supernatants of the two separations; performing ultra-filtration to the combined supernatants then spray-drying to obtain the water-soluble dietary fiber; then washing, drying and crushing the water-insoluble dietary fiber of the precipitates of the two centrifugal separations. The preparation method utilizes liquid-state fermentation, so that the preparation method is mild in reaction condition, free of metal catalyst, capable of inhibiting harmful bacteria and reducing toxin content, simple in process and equipment as well as convenient to operate; moreover, the preparation method utilizes spray-drying, so that the preparation method is free of the steps of vacuum concentration, alcohol precipitation, drying and the like; thus, the preparation has greatly reduced product cost.
Description
Technical field
The present invention relates to a kind of method utilizing fermentable to prepare soya-dregs water-soluble dietary fiber.
Background technology
Dietary fiber is called as " the 7th nutrient " of needed by human, the same with proteins,vitamins,minerals essential to health.In nutrition educational circles, dietary fiber is called as " green street cleaner ", and human body intestinal canal can be kept unobstructed, toxin expelling defaecation, fat eliminating beauty treatment, safeguards skin health.Brainstrust is unanimously thought: dietary fiber will be that 21 worlds dominate one of food.
Dietary fiber can be divided into water-soluble and insoluble diedairy fiber according to dissolubility, and the two all has specific physiological function and health-care efficacy in human body, but specifically acts on difference.The effect of insoluble composition is considered to mainly be to make enteron aisle produce mechanical creeping effect, can prevent obesity, constipation, intestinal cancer etc.Water soluble ingredient then has larger facilitation to physiological metabolism, as prevented gall stone, get rid of harmful metal ion, prevent diabetes, reduce serum and hepatic cholesterol, suppress postprandial blood sugar rise and prevent hypertension and heart disease etc.The soluble component content of high-quality dietary fiber should reach more than 10%, there is good dilatancy and retentiveness, such product just possesses strong physiologically active, has obvious health-care effect, can as the additive of various food and for the production of health food.
China is major country of production and the product consumption state of soybean, and bean product processing total scale is very large, and soybean slag output is very considerable.Soy bean dregs dietary fiber is considered to foolproof dietary fiber sources and draws attention, though but in soybean slag the content of dietary fiber up to more than 50%, but wherein majority is insoluble diedairy fiber, and water soluble dietary fiber content very low (only accounting for about 3% of raw material), this physiological function that greatly have impact on soy bean dregs dietary fiber is active, thus limits its scope of application.Therefore the preparation method researching and developing soya-dregs water-soluble dietary fiber has important practical significance.
Summary of the invention
The technical solution used in the present invention is summarized as follows:
Fermentable is utilized to prepare the method for soya-dregs water-soluble dietary fiber, adopt with the obtained soya-dregs water-soluble dietary fiber of fermentable, in conjunction with HTHP boiling and fermentable metabolin to coarse-fibred modification, improve water-soluble dietary fiber yield, ultrafiltration dialysis, concentrated and spraying dry are carried out to the water-soluble dietary fiber feed liquid obtained.
Step is as follows:
(1) strain domestication mode
1) get a fresh slant activation bacterial classification, add 20mL physiological saline, scrape spore with oese, make spore suspension, get 1mL spore suspension and be inoculated in and be equipped with in the 250mL triangular flask of 60mL fermentation medium, add certain amount of CaCO
3, 34 DEG C, 60h cultivated by 200r/min shaking table, activates 2 times;
2) bacterial classification of step 1) being got 1mL accesses in 60mL mixed liquor (soybean slag: water=1:1), and 60h cultivated by 200r/min shaking table, and soybean slag is wet basis, and water content is 80%;
3) by step 2) in bacterial classification get 1mL and access in 100mL mixed liquor (soybean slag: water=1:9), 60h cultivated by 200r/min shaking table.Bacterial classification sum reaches 10
9individual/mL, as leavening;
(2) soybean slag: water=1:20-30, soybean slag is wet basis, and water content is 80%.Cross colloid mill at least 1 time, adjustment pH is 6-6.5, and be placed in high-pressure sterilizing pot 121 DEG C, sterilizing 20min, as fermentation medium;
(3) by the culture medium of Rhizopus oryzae access step (2) after domestication, inoculum concentration is 5%-7%, 34 DEG C, static gas wave refrigerator 5-7d;
(4) high-pressure sterilizing pot 121 DEG C is placed in the culture medium in step (3), sterilizing 20min;
(5) undertaken centrifugal by the mixed liquor in step (4), centrifugal speed is 3500rpm, and the centrifugal time is 30min, and retained by supernatant after centrifugal, sediment is dispersed in 2 times of water and carries out stirring boiling, and the time is 30min;
(6) undertaken centrifugal by the mixed liquor in step (5), centrifugal speed is 3500rpm, and the time is 30min, is retained by supernatant after centrifugal, and sediment is soya-dregs water insoluble diedairy fiber through washing, drying, pulverizing;
(7) by the supernatant collection of step (5), (6) to carrying out ultrafiltration together, ultrafiltration can be selected and be retained the milipore filter that relative molecular weight is 1K ~ 10KDa, liquor inlet pressure 3 × 10
5pa, outlet pressure 0.5 × 10
5pa, after carrying out ultrafiltration dialysis 2h with pure water, be concentrated into that trapped fluid volume is feed liquid original volume 20% ~ 25%; Spray-dired inlet temperature is 135 DEG C, and outlet temperature is 80 DEG C, and what obtain is soya-dregs water-soluble dietary fiber.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated:
Embodiment 1:
1. get soybean slag 500g(wet basis, moisture 80%), the amount being 1:20 by solid-liquid ratio adds water mixing, and through 2 milling treatment of colloid, regulate pH to be 6.0, be placed in high-pressure sterilizing pot 121 DEG C, sterilizing 20min, as fermentation medium;
2. access the Rhizopus oryzae leavening prepared, addition is 5%, 34 DEG C, static gas wave refrigerator 5d; Cultivate end and to be placed in high-pressure sterilizing pot 121 DEG C, sterilizing 20min; To mixed liquor centrifugation, 3500rpm, centrifugal 30min, collect supernatant, sediment;
3. add water by 2 times amount of sediment quality, stir boiling 30min; Centrifugation again, 3500rpm, centrifugal 30min, collect supernatant, sediment; Sediment is soya-dregs water insoluble diedairy fiber through washing, drying, pulverizing;
4. collect twice centrifugal after supernatant, carry out ultrafiltration dialysis, concentrated to supernatant, collect ultra-filter retentate, ultrafiltration is selected and is retained the milipore filter that relative molecular weight is 1KDa, liquor inlet pressure 3 × 10
5pa, outlet pressure 0.5 × 10
5pa, after carrying out ultrafiltration dialysis 2h with pure water, to be concentrated into trapped fluid volume be time of the 20%-25% of feed liquid original volume is 9h;
5. trapped fluid carries out spraying dry, inlet temperature 135 DEG C, outlet temperature 80 DEG C, collects spray dried powder, obtains water-soluble dietary fiber powder-product;
Product yield (water-soluble dietary fiber accounts for gross mass percentage) is 25.7%, and measuring water soluble dietary fiber content in product through AOAC method is 83.8%, protein content 1.5%, fat content 0.2%, content of ashes 1.1%.
Embodiment 2:
1. get soybean slag 500g(wet basis, moisture 80%), the amount being 1:30 by solid-liquid ratio adds water mixing, and through 2 milling treatment of colloid, regulate pH to be 6.5, be placed in high-pressure sterilizing pot 121 DEG C, sterilizing 20min, as fermentation medium;
2. access the Rhizopus oryzae leavening prepared, addition is 7%, 34 DEG C, static gas wave refrigerator 7d; Cultivate end and to be placed in high-pressure sterilizing pot 121 DEG C, sterilizing 20min; To mixed liquor centrifugation, 3500rpm, centrifugal 30min, collect supernatant, sediment;
3. add water by 2 times amount of sediment quality, stir boiling 30min; Centrifugation again, 3500rpm, centrifugal 30min, collect supernatant, sediment; Sediment is soya-dregs water insoluble diedairy fiber through washing, drying, pulverizing;
4. collect twice centrifugal after supernatant, carry out ultrafiltration dialysis, concentrated to supernatant, collect ultra-filter retentate, ultrafiltration is selected and is retained the milipore filter that relative molecular weight is 1KDa, liquor inlet pressure 3 × 10
5pa, outlet pressure 0.5 × 10
5pa, after carrying out ultrafiltration dialysis 2h with pure water, to be concentrated into trapped fluid volume be time of the 20%-25% of feed liquid original volume is 9h;
5. trapped fluid carries out spraying dry, inlet temperature 135 DEG C, outlet temperature 80 DEG C, collects spray dried powder, obtains water-soluble dietary fiber powder-product;
Product yield (water-soluble dietary fiber accounts for gross mass percentage) is 26.5%, and measuring water soluble dietary fiber content in product through AOAC method is 84.5%, protein content 1.3%, fat content 0.3%, content of ashes 0.9%.
Claims (2)
1. utilize fermentable to prepare the method for soya-dregs water-soluble dietary fiber, adopt with the obtained soya-dregs water-soluble dietary fiber of fermentable, in conjunction with HTHP boiling and fermentable metabolin to coarse-fibred modification, improve water-soluble dietary fiber yield, ultrafiltration dialysis, concentrated and spraying dry are carried out to the water-soluble dietary fiber feed liquid obtained.
2. utilize fermentable to prepare the method for soya-dregs water-soluble dietary fiber, comprise the steps:
(1) strain domestication mode
1) get a fresh slant activation bacterial classification, add 20mL physiological saline, scrape spore with oese, make spore suspension, get 1mL spore suspension and be inoculated in and be equipped with in the 250mL triangular flask of 60mL fermentation medium, add certain amount of CaCO
3, 34 DEG C, 60h cultivated by 200r/min shaking table, activates 2 times;
2) bacterial classification of step 1) being got 1mL accesses in 60mL mixed liquor (soybean slag: water=1:1), and 60h cultivated by 200r/min shaking table, and soybean slag is wet basis, and water content is 80%;
3) by step 2) in bacterial classification get 1mL and access in 100mL mixed liquor (soybean slag: water=1:9), 60h cultivated by 200r/min shaking table; Bacterial classification sum reaches 10
9individual/mL, as leavening;
(2) soybean slag: water=1:20-30, soybean slag is wet basis, and water content is 80%; Cross colloid mill at least 1 time, adjustment pH is 6-6.5, and be placed in high-pressure sterilizing pot 121 DEG C, sterilizing 20min, as fermentation medium;
(3) by the culture medium of Rhizopus oryzae access step (2) after domestication, inoculum concentration is 5%-7%, 34 DEG C, static gas wave refrigerator 5-7d;
(4) high-pressure sterilizing pot 121 DEG C is placed in the culture medium in step (3), sterilizing 20min;
(5) undertaken centrifugal by the mixed liquor in step (4), centrifugal speed is 3500rpm, and the centrifugal time is 30min, and retained by supernatant after centrifugal, sediment is dispersed in 2 times of water and carries out stirring boiling, and the time is 30min;
(6) undertaken centrifugal by the mixed liquor in step (5), centrifugal speed is 3500rpm, and the time is 30min, is retained by supernatant after centrifugal, and sediment is soya-dregs water insoluble diedairy fiber through washing, drying, pulverizing;
(7) by the supernatant collection of step (5), (6) to carrying out ultrafiltration together, ultrafiltration can be selected and be retained the milipore filter that relative molecular weight is 1K ~ 10KDa, liquor inlet pressure 3 × 10
5pa, outlet pressure 0.5 × 10
5pa, after carrying out ultrafiltration dialysis 2h with pure water, be concentrated into that trapped fluid volume is feed liquid original volume 20% ~ 25%; Spray-dired inlet temperature is 135 DEG C, and outlet temperature is 80 DEG C, and what obtain is soya-dregs water-soluble dietary fiber.
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Cited By (3)
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| CN109170429A (en) * | 2018-09-06 | 2019-01-11 | 山东禹王生态食业有限公司 | A kind of preparation method of modified bean dregs dietary fiber beverage |
| CN112385851A (en) * | 2020-11-16 | 2021-02-23 | 东北农业大学 | Method for preparing soluble dietary fiber powder by using bean dregs |
| CN114041561A (en) * | 2021-11-18 | 2022-02-15 | 江南大学 | Method for producing low-GI red yeast powder by fermenting yellow wine lees with monascus |
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| CN109170429A (en) * | 2018-09-06 | 2019-01-11 | 山东禹王生态食业有限公司 | A kind of preparation method of modified bean dregs dietary fiber beverage |
| CN112385851A (en) * | 2020-11-16 | 2021-02-23 | 东北农业大学 | Method for preparing soluble dietary fiber powder by using bean dregs |
| CN114041561A (en) * | 2021-11-18 | 2022-02-15 | 江南大学 | Method for producing low-GI red yeast powder by fermenting yellow wine lees with monascus |
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