CN106360743A - Extraction method of sea asparagus dietary fiber - Google Patents
Extraction method of sea asparagus dietary fiber Download PDFInfo
- Publication number
- CN106360743A CN106360743A CN201610860772.1A CN201610860772A CN106360743A CN 106360743 A CN106360743 A CN 106360743A CN 201610860772 A CN201610860772 A CN 201610860772A CN 106360743 A CN106360743 A CN 106360743A
- Authority
- CN
- China
- Prior art keywords
- dietary fiber
- salicornia bigelovii
- bigelovii torr
- filtrate
- filtering residue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 89
- 238000000605 extraction Methods 0.000 title claims abstract description 33
- 235000005340 Asparagus officinalis Nutrition 0.000 title claims abstract description 18
- 244000003416 Asparagus officinalis Species 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 42
- 102000004190 Enzymes Human genes 0.000 claims abstract description 24
- 108090000790 Enzymes Proteins 0.000 claims abstract description 24
- 239000000843 powder Substances 0.000 claims abstract description 22
- 241000234427 Asparagus Species 0.000 claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 10
- 229930006000 Sucrose Natural products 0.000 claims abstract description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 8
- 235000020121 low-fat milk Nutrition 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 4
- 241000201920 Salicornia bigelovii Species 0.000 claims description 76
- 239000000706 filtrate Substances 0.000 claims description 47
- 238000001914 filtration Methods 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 38
- 239000012531 culture fluid Substances 0.000 claims description 29
- 241000894006 Bacteria Species 0.000 claims description 25
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 19
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 13
- 150000001875 compounds Chemical class 0.000 claims description 11
- 235000012054 meals Nutrition 0.000 claims description 10
- 239000004382 Amylase Substances 0.000 claims description 9
- 108010065511 Amylases Proteins 0.000 claims description 9
- 102000013142 Amylases Human genes 0.000 claims description 9
- 241000222481 Schizophyllum commune Species 0.000 claims description 9
- 235000019418 amylase Nutrition 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 9
- 239000002054 inoculum Substances 0.000 claims description 9
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 9
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 239000000835 fiber Substances 0.000 claims description 7
- 230000001376 precipitating effect Effects 0.000 claims description 7
- 238000001556 precipitation Methods 0.000 claims description 7
- 238000010298 pulverizing process Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- 208000031481 Pathologic Constriction Diseases 0.000 claims description 6
- 241001149558 Trichoderma virens Species 0.000 claims description 6
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- 210000001215 vagina Anatomy 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 230000002255 enzymatic effect Effects 0.000 abstract description 4
- 230000014759 maintenance of location Effects 0.000 abstract description 4
- 229920002472 Starch Polymers 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 239000008107 starch Substances 0.000 abstract description 2
- 235000019698 starch Nutrition 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 2
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 238000005238 degreasing Methods 0.000 abstract 1
- 235000013681 dietary sucrose Nutrition 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 239000013049 sediment Substances 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 19
- 230000008569 process Effects 0.000 description 13
- 238000011081 inoculation Methods 0.000 description 7
- 239000001913 cellulose Substances 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 241000201895 Salicornia Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- -1 chromocor compound Chemical class 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 108010059892 Cellulase Proteins 0.000 description 2
- 241000223261 Trichoderma viride Species 0.000 description 2
- 230000000767 anti-ulcer Effects 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000036186 satiety Effects 0.000 description 2
- 235000019627 satiety Nutrition 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 230000004992 fission Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229940059442 hemicellulase Drugs 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000004223 radioprotective effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to extraction of dietary fiber, in particular to an extraction method of sea asparagus dietary fiber. The extraction method comprises the following steps of S1, cleaning sea asparagus, drying, smashing, sieving and degreasing; S2, quickly extracting and separating to obtain filter liquor and a filter residue; S3, adding low-fat milk powder and saccharose into the filter liquor, homogenizing and sterilizing to obtain a culture solution; S4, inoculating a mixed culture into the culture solution, and culturing to obtain a fermentation agent; S5, adjusting pH of the filter liquor, then adding complex enzyme, and obtaining enzymatic hydrolysate through enzymolysis; S6, enzyme denaturing and cooling the enzymatic hydrolysate, inoculating the fermentation agent, culturing, and centrifugally separating to obtain filter liquor and a filter residue; S7, washing the filter residue with water, and drying to obtain insoluble dietary fiber; adding ethyl alcohol into the filter liquor, collecting sediments, and drying to obtain water-soluble dietary fiber; S8, mixing the water-soluble dietary fiber and the insoluble dietary fiber. According to the extraction method of the sea asparagus dietary fiber provided by the invention, the reaction condition is moderate, other impurities such as protein and starch can be effectively removed, the yield and the purity of the dietary fiber are improved, and the retention ability and the expansibility of the dietary fiber are improved.
Description
Technical field
The present invention relates to a kind of dietary fiber, more particularly, to a kind of extracting method of Salicornia Bigelovii Torr. dietary fiber.
Background technology
The Carnified euhalophyte of no base of leaf that Salicornia Bigelovii Torr. (salicornia) belongs to for Chenopodiaceae salicornia europaeal, the tolerance of salinity is high,
It is commonly called as marine borer grass, salicornia europaeal, Salicornia Bigelovii Torr. or extra large bean, be more common in lick, seabeach is taken up in salt lake.Recently as both at home and abroad to sea
Deepening continuously of Germinatus Phragmitiss research, has separately obtained polysaccharide, chromocor compound, biogenic salt, chlorophyll etc. multiple from Salicornia Bigelovii Torr.
There is the active component of medical value and health value.Show that chromocor compound has vessel softening, blood sugar lowering, fall by research
Blood fat, antioxidation, removing interior free yl, defying age, resisting pathogenic microbes, infection, anti-inflammatory, antiulcer, antiallergic, rush
Enter the different physiological roles such as tissue regeneration;Polysaccharide has antitumor, antiviral, antiulcer, radioprotective, strengthens immunity, resisting fatigue
The effects such as.
Dietary fiber is described as the seventh-largest nutrient of the mankind, has important function to maintaining human health diet.In recent years
Carry out the raising with people's living standard, diet structure has a very large change, dietary fiber is as a kind of functional food
Base material, increasingly receives significant attention;Dietary fiber divides according to dissolubility and can be divided into water soluble dietary fiber (sdf) and insoluble
Property dietary fiber (idf);Water soluble dietary fiber (sdf) is the class meals that a class may be dissolved in 100 DEG C of water of ph6~7
Stored substance in fiber, predominantly plant cell and secretions, additionally include microbial polysaccharide and synthesis polysaccharide, its composition
A mainly class gelatin substance and glucide.Sdf can participate in and affect the multiple metabolism in human body, such as lipid metabolism, carbon water
Compound metabolism;Stomach satiety can be increased, reduce food intake dose, there is the effect of prevention obesity.Insoluble meals are fine
Dimension (idf) is the insoluble class dietary fiber in 100 DEG C of water of ph6~7 of a class, and its composition is cellulose, half fiber
Element, lignin, Pectin, proto and shitosan etc..Idf can increase stomach satiety, contributes to enterokinesia, can change as dredging constipation
Solution calculus, loses weight, prevents the functional food of intestinal cancer.
Extract dietary fiber at present from Salicornia Bigelovii Torr., typically extracted using the method that alkaline process or alkaline process are combined with enzyme process,
But using alkaline process due to immersion repeatedly and frequently heat treatment, retention ability and the expansive force of diet fiber product can be reduced,
In addition the alkalies cognition discharged causes environmental pollution;Use Enzymatic Extraction dietary fiber, process time is long;And said method is all
A problem is that gained dietary fiber low yield, purity is low.It is therefore desirable to it is fine to set up a kind of extraction Salicornia Bigelovii Torr. meals
The new method of dimension.
Content of the invention
The technical problem to be solved is: in view of the shortcomings of the prior art, provides a kind of Salicornia Bigelovii Torr. meals
The extracting method of fiber, this method can improve extraction ratio and the purity of dietary fiber, improves retention ability and the expansive force of dietary fiber.
For solving above-mentioned technical problem, the technical scheme is that
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, described extracting method comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder;
S2. defat Salicornia Bigelovii Torr. dry powder in s1 is taken to add volumetric concentration for 5~% according to solid-liquid ratio 1:5~1:10, mixing is all
Carry out homogenate extraction after even, separate and obtain filtering residue and filtrate;
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 1~3% and 0.5~1% sucrose, after mixing well
Sterilize at 100~110 DEG C 3~5min, obtains culture fluid;
S4. take the culture fluid in s3, then lactic acid bacteria, Hypocrea virens and Schizophyllum commune Franch. are inoculated in culture fluid, 22~
At 37 DEG C, culture 4~8h obtains leaven;
S5. take the filtering residue that s2 obtains, adjust ph to 4~5.5, then add the compound of described filtering residue weight 0.1~0.5%
Enzyme, obtains enzymolysis solution through enzymolysis;
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, the leaven of inoculation s4 preparation, then at 25~37 DEG C
Lower culture 12~36h, then stops fermentation, is centrifugally separating to obtain filtrate and filtering residue;
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber;
Add the ethanol that 5~8 times of volumetric concentrations of filtrate volume are 65~85%, stirring, standing, precipitating in filtrate, collect precipitation, be dried
Obtain sea asparagus water-solubility dietary fiber;
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
As a kind of improved technical scheme, the extraction time 1~3min of described homogenate extraction, Extracting temperature 50~60
DEG C, extract voltage 165~220v.
As a kind of improved technical scheme, in step s4 the inoculum concentration of mixed bacteria be culture fluid weight 1.5~
3%.
As a kind of improved technical scheme, in step s4, mixed bacteria is by lactic acid bacteria, Hypocrea virens, sturdy wheat stricture of vagina born of the same parents
Bacterium and Schizophyllum commune Franch. mix according to mass ratio 0.5~1:0.5~1.2:0.4~0.7:0.3~0.7 ratio.
As a kind of improved technical scheme, in step s5, compound enzyme includes protease and a~amylase, and described albumen
Enzyme and a~amylase mix according to the ratio of 0.5~1.2:0.4~0.7.
As a kind of improved technical scheme, in step s6, the inoculum concentration of leaven is the 2~4% of enzymolysis solution weight.
As a kind of preferred technical scheme, step s5 is digested using ultrasonic wave added mode, ultrasonic power 300~600w,
Ultrasonic time 5~15min.
After employing technique scheme, the invention has the beneficial effects as follows:
Compared with prior art, the present invention mainly has the advantage that
(1) present invention, with Salicornia Bigelovii Torr. as raw material, carries out dietary fiber extraction, takes full advantage of Salicornia Bigelovii Torr. active component content
Feature abundant, that comprehensive nutrient is high, has established technical foundation for exploitation dietary fiber.
(2) using volumetric concentration, the ethanol for 5%-15% carries out homogenate extraction to Salicornia Bigelovii Torr., can make flavone in Salicornia Bigelovii Torr.
Compound isoreactivity composition fully extracts, and as culture fluid, prepared by inoculation strain for the filtrate then being obtained with after homogenate extraction
Leaven;Again process is carried out to the filtering residue of homogenate extraction using ultrasonic wave added enzymatic isolation method and obtain enzymolysis solution, this process is using compound
Enzyme can remove the starch in Salicornia Bigelovii Torr., also protein is effectively degraded simultaneously, improves the purity of dietary fiber;Then again
Leaven is inoculated in enzymolysis solution by fermentation, because lactic acid bacteria produces substantial amounts of organic acid in sweat, fiber can be made
The glucosides bond fission of element, reduces the macromolecular polymeric degree of dietary fiber, improves the content of water soluble dietary fiber;Green wood
Mycete is combined the Secretory cycle that sturdy wheat stricture of vagina born of the same parents bacterium fermentation can extend cellulase, contributes to cellulose and fully degrades;In addition split
Gill fungus also can secrete substantial amounts of cellulase and hemicellulase in fermentation, and three's synergic fermentation reduces cellulose and half fibre
Dimension cellulose content, and also improve the molten of Salicornia Bigelovii Torr. dietary fiber while the other active components in retaining Salicornia Bigelovii Torr. are active
Swollen property;
(3) dietary fiber that the present invention is extracted with Salicornia Bigelovii Torr. for raw material, the method pair being combined with fermentation method using enzyme process
Dodge the filtrate after carrying and filtering residue is processed respectively, substantially increase dietary fiber yield and purity, further improve dietary fiber simultaneously
Physicochemical property, this research dietary fiber yield is up to 45.6%, and purity is 92.8%;The retention ability of gained dietary fiber up to
12.16g/g, expansive force is up to 16.8ml/g;Reaction condition is gentle, and dietary fiber loss is few.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
Example 1
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder;
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add, according to solid-liquid ratio 1:5, the ethanol that volumetric concentration is 5%, mixing is all
Carry out homogenate extraction (extraction time 1min, 50 DEG C of Extracting temperature extract voltage 165v) after even, separate and obtain filtering residue and filtrate;
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 1.5% and 0.5% sucrose, after mixing well
Sterilize at 100 DEG C 3min, obtains culture fluid;
S4. take the culture fluid in s3, then by the 1.5% of culture fluid weight mixed bacteria (lactic acid bacteria, Trichoderma viride
Bacterium, sturdy wheat stricture of vagina born of the same parents bacterium and Schizophyllum commune Franch. mix according to the ratio of 0.6:0.7:0.5:0.3) it is inoculated in culture fluid, train at 23 DEG C
Foster 5h obtains leaven;
S5. take the filtering residue that s2 obtains, adjust ph to 4, then add compound enzyme (protease and a of described filtering residue weight 0.1%
~amylase is mixed in the ratio of 0.7:0.4), obtain through ultrasonic wave added enzymolysis (ultrasonic power 360w, ultrasonic time 5min)
Enzymolysis solution;
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
The 2% of liquid weight), then cultivate 24h at 28 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue;
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber;
Add the ethanol that 6 times of volumetric concentrations of filtrate volume are 65%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber;
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 41.9%, and purity is 89.3%, and gained meals are fine
Maintain waterpower 10.2g/g, expansive force 14.2ml/g.
Example 2
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder;
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add, according to solid-liquid ratio 1:8, the ethanol that volumetric concentration is 8%, mixing is all
Carry out homogenate extraction (extraction time 1.5min, 52 DEG C of Extracting temperature extract voltage 170v) after even, separate and obtain filtering residue and filter
Liquid;
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 1.8% and 0.7% sucrose, after mixing well
Sterilize at 108 DEG C 3min, obtains culture fluid;
S4. take the culture fluid in s3, then by the 1.7% of culture fluid weight mixed bacteria (lactic acid bacteria, Hypocrea virens
Mix according to the ratio of 0.8:0.7:0.6:0.5 with Schizophyllum commune Franch.) it is inoculated in culture fluid, at 25 DEG C, culture 6h obtains leaven;
S5. take the filtering residue that s2 obtains, adjust ph to 4.5, then add filtering residue weight 0.2% compound enzyme (protease and a~
Amylase is mixed in the ratio of 1:0.7), digested through ultrasonic wave added enzymolysis (ultrasonic power 400w, ultrasonic time 13min)
Liquid;
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
The 2.5% of liquid weight), then cultivate 28h at 30 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue;
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber;
Add the ethanol that 7 times of volumetric concentrations of filtrate volume are 70%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber;
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 42.8%, and purity is 89.9%, and gained meals are fine
Maintain waterpower 10.8g/g, expansive force 14.9ml/g.
Example 3
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder;
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add the ethanol that volumetric concentration is 10%, mixing according to solid-liquid ratio 1:10
Carry out homogenate extraction (extraction time 1.8min, 55 DEG C of Extracting temperature extract voltage 180v) after uniformly, separate and obtain filtering residue and filter
Liquid;
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 2% and 0.5% sucrose, 110 after mixing well
Sterilize at DEG C 3min, obtains culture fluid;
S4. take the culture fluid in s3, then that the mixed bacteria of culture fluid weight 2% is (lactic acid bacteria, Hypocrea virens, thick
Strong wheat stricture of vagina born of the same parents bacterium and Schizophyllum commune Franch. mix according to the ratio of 1:1:0.4:0.7) it is inoculated in culture fluid, at 28 DEG C, culture 7h is sent out
Ferment agent;
S5. take the filtering residue that s2 obtains, adjust ph to 4.8, then add filtering residue weight 0.3% compound enzyme (protease and a~
Amylase is mixed in the ratio of 0.7:0.6), obtain enzyme through ultrasonic wave added enzymolysis (ultrasonic power 450w, ultrasonic time 8min)
Solution liquid;
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
The 3% of liquid weight), then cultivate 30h at 32 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue;
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber;
Add the ethanol that 7 times of volumetric concentrations of filtrate volume are 75%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber;
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 43.9%, and purity is 90.3%, and gained meals are fine
Maintain waterpower 11.2g/g, expansive force 15.2ml/g.
Example 4
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder;
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add the ethanol that volumetric concentration is 10%, mixing according to solid-liquid ratio 1:8
Carry out homogenate extraction (extraction time 2.5min, 60 DEG C of Extracting temperature extract voltage 200v) after uniformly, separate and obtain filtering residue and filter
Liquid;
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 2.5% and 0.8% sucrose, after mixing well
Sterilize at 105 DEG C 4.5min, obtains culture fluid;
S4. take the culture fluid in s3, then by the 2.5% of culture fluid weight mixed bacteria (lactic acid bacteria, Trichoderma viride
Bacterium, sturdy wheat stricture of vagina born of the same parents bacterium and Schizophyllum commune Franch. mix according to the ratio of 1:1:0.7:0.7) it is inoculated in culture fluid, cultivate 5h at 30 DEG C
Obtain leaven;
S5. take the filtering residue that s2 obtains, adjust ph to 5.5, then add compound enzyme (protease and a of filtering residue weight 0.45%
~amylase is mixed in the ratio of 0.5:0.7), obtain through ultrasonic wave added enzymolysis (ultrasonic power 500w, ultrasonic time 12min)
Enzymolysis solution;
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
Liquid weight 3.5%), then cultivate 36h at 35 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue;
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber;
Add the ethanol that 8 times of volumetric concentrations of filtrate volume are 80%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber;
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 45.6%, and purity is 92.8%, and gained meals are fine
Maintain waterpower 12.16g/g, expansive force 16.8ml/g.
Example 5
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder;
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add the ethanol that volumetric concentration is 15%, mixing according to solid-liquid ratio 1:10
Carry out homogenate extraction (extraction time 3min, 60 DEG C of Extracting temperature extract voltage 220v) after uniformly, separate and obtain filtering residue and filter
Liquid;
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 3% and 1% sucrose, at 105 DEG C after mixing well
Lower sterilizing 3min, obtains culture fluid;
S4. take the culture fluid in s3, then by the 3% of culture fluid weight mixed bacteria (lactic acid bacteria, Hypocrea virens and
Schizophyllum commune Franch. mixes according to the ratio of 1:1.2:0.7:0.7) it is inoculated in culture fluid, at 28 DEG C, culture 5h obtains leaven;
S5. take the filtering residue that s2 obtains, adjust ph to 5.2, then add filtering residue weight 0.5% compound enzyme (protease and a~
Amylase is mixed in the ratio of 1.2:0.7), obtain enzyme through ultrasonic wave added enzymolysis (ultrasonic power 560w, ultrasonic time 10min)
Solution liquid;
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
Liquid weight 4%), then cultivate 36h at 37 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue;
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber;
Add the ethanol that 8 times of volumetric concentrations of filtrate volume are 85%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber;
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 45.2%, and purity is 92.3%, and gained meals are fine
Maintain waterpower 11.85%, expansive force 16.2ml/g.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (7)
1. a kind of extracting method of Salicornia Bigelovii Torr. dietary fiber is it is characterised in that described extracting method comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain defat
Salicornia Bigelovii Torr. dry powder;
S2. defat Salicornia Bigelovii Torr. dry powder in s1 is taken to add, according to solid-liquid ratio 1:5~1:10, the ethanol that volumetric concentration is 5%~15%,
Carry out homogenate extraction after mix homogeneously, separate and obtain filtering residue and filtrate;
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 1~3% and 0.5~1% sucrose, after mixing well
Sterilize at 100~110 DEG C 3~5min, obtains culture fluid;
S4. take the culture fluid in s3, then mixed bacteria is inoculated in culture fluid, culture 4~8h obtains at 22~37 DEG C
Leaven;
S5. take the filtering residue that s2 obtains, adjust ph to 4~5.5, then add the compound enzyme of described filtering residue weight 0.1~0.5%, warp
Cross enzymolysis and obtain enzymolysis solution;
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, inoculate the leaven of s4 preparation, then train at 25~37 DEG C
Support 12~36h, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue;
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber;Filtrate
Middle addition 5~8 times of volumetric concentrations of filtrate volume are 65~85% ethanol, and stirring, standing, precipitating are collected precipitation, be dried to obtain
Sea asparagus water-solubility dietary fiber;
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr. meals
Fiber.
2. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: described homogenate extraction
Extraction time 1~3min, 50~60 DEG C of Extracting temperature, extract voltage 165~220v.
3. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: in step s4 mix
The inoculum concentration of strain is the 1.5~3% of culture fluid weight.
4. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: in step s4 mix
Strain by lactic acid bacteria, Hypocrea virens, sturdy wheat stricture of vagina born of the same parents bacterium and Schizophyllum commune Franch. according to mass ratio 0.5~1:0.5~1.2:0.4~
0.7:0.3~0.7 ratio mixes.
5. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: compound in step s5
Enzyme includes protease and a~amylase, and described protease and a~amylase mix according to the ratio of 0.5~1.2:0.4~0.7
Close.
6. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: step s5 is using super
Sound supplementary mode digests, ultrasonic power 300~600w, ultrasonic time 5~15min.
7. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: in step s6 ferment
The inoculum concentration of agent is the 2~4% of enzymolysis solution weight.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610860772.1A CN106360743A (en) | 2016-09-28 | 2016-09-28 | Extraction method of sea asparagus dietary fiber |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610860772.1A CN106360743A (en) | 2016-09-28 | 2016-09-28 | Extraction method of sea asparagus dietary fiber |
Publications (1)
Publication Number | Publication Date |
---|---|
CN106360743A true CN106360743A (en) | 2017-02-01 |
Family
ID=57898384
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610860772.1A Pending CN106360743A (en) | 2016-09-28 | 2016-09-28 | Extraction method of sea asparagus dietary fiber |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106360743A (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107969708A (en) * | 2017-11-29 | 2018-05-01 | 马斌祥 | A kind of method that water-soluble dietary fiber is extracted from hybrid giant napier |
CN108925790A (en) * | 2018-06-20 | 2018-12-04 | 四川农业大学 | A kind of preparation method of high fine orange beverage |
CN108936700A (en) * | 2018-06-19 | 2018-12-07 | 四川省农业科学院土壤肥料研究所 | A method of extracting dietary fiber from hickory chick |
CN109170918A (en) * | 2018-10-09 | 2019-01-11 | 山西省农业科学院果树研究所 | A kind of preparation method and preparation system of jujube dietary fiber |
CN110124619A (en) * | 2019-05-16 | 2019-08-16 | 重庆三峡学院 | A kind of preparation method of heavy metal lead adsorbent |
CN114431401A (en) * | 2022-02-28 | 2022-05-06 | 江西省农业科学院农产品加工研究所 | Preparation method of asparagus polysaccharide emulsion and application of asparagus polysaccharide emulsion in rice flour anti-aging |
CN114847494A (en) * | 2022-04-20 | 2022-08-05 | 湖南农业大学 | Method for extracting dietary fiber from reed shoots |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103445190A (en) * | 2013-09-12 | 2013-12-18 | 江门市浩伦生态农业有限公司 | Method for extracting dietary fibers from dendrobium officinale |
CN103549215A (en) * | 2013-11-15 | 2014-02-05 | 双胞胎(集团)股份有限公司 | Intensive pig farm weanling pig feed and feeding mode thereof |
CN104957640A (en) * | 2015-06-26 | 2015-10-07 | 北京京隆卓尚投资有限公司 | Cistanche deserticola dietary fiber and preparation method thereof |
CN106172675A (en) * | 2016-09-28 | 2016-12-07 | 潍坊友容实业有限公司 | A kind of Salicornia Bigelovii Torr. dietary-fiber biscuit and preparation method thereof |
-
2016
- 2016-09-28 CN CN201610860772.1A patent/CN106360743A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103445190A (en) * | 2013-09-12 | 2013-12-18 | 江门市浩伦生态农业有限公司 | Method for extracting dietary fibers from dendrobium officinale |
CN103549215A (en) * | 2013-11-15 | 2014-02-05 | 双胞胎(集团)股份有限公司 | Intensive pig farm weanling pig feed and feeding mode thereof |
CN104957640A (en) * | 2015-06-26 | 2015-10-07 | 北京京隆卓尚投资有限公司 | Cistanche deserticola dietary fiber and preparation method thereof |
CN106172675A (en) * | 2016-09-28 | 2016-12-07 | 潍坊友容实业有限公司 | A kind of Salicornia Bigelovii Torr. dietary-fiber biscuit and preparation method thereof |
Non-Patent Citations (2)
Title |
---|
艾仄宜等: "茶叶水溶性膳食纤维的制备及其理化特性的研究", 《茶叶科学》 * |
谢碧霞等: "竹笋膳食纤维开发和主要功能性质的研究", 《经济林研究》 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107969708A (en) * | 2017-11-29 | 2018-05-01 | 马斌祥 | A kind of method that water-soluble dietary fiber is extracted from hybrid giant napier |
CN108936700A (en) * | 2018-06-19 | 2018-12-07 | 四川省农业科学院土壤肥料研究所 | A method of extracting dietary fiber from hickory chick |
CN108925790A (en) * | 2018-06-20 | 2018-12-04 | 四川农业大学 | A kind of preparation method of high fine orange beverage |
CN109170918A (en) * | 2018-10-09 | 2019-01-11 | 山西省农业科学院果树研究所 | A kind of preparation method and preparation system of jujube dietary fiber |
CN110124619A (en) * | 2019-05-16 | 2019-08-16 | 重庆三峡学院 | A kind of preparation method of heavy metal lead adsorbent |
CN114431401A (en) * | 2022-02-28 | 2022-05-06 | 江西省农业科学院农产品加工研究所 | Preparation method of asparagus polysaccharide emulsion and application of asparagus polysaccharide emulsion in rice flour anti-aging |
CN114431401B (en) * | 2022-02-28 | 2023-07-04 | 江西省农业科学院农产品加工研究所 | Preparation method of asparagus polysaccharide emulsion and application of asparagus polysaccharide emulsion in rice flour aging resistance |
CN114847494A (en) * | 2022-04-20 | 2022-08-05 | 湖南农业大学 | Method for extracting dietary fiber from reed shoots |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106360743A (en) | Extraction method of sea asparagus dietary fiber | |
CN101775376B (en) | Compound bamboo shoot wall breaking enzyme and preparation method of bamboo shoot dietary fiber | |
CN106387924A (en) | Method for extracting dietary fibers for suaeda salsa | |
CN104187746B (en) | The technique that fermentation method prepares Testa Phaseoli radiati water soluble dietary fiber | |
CN103190576B (en) | Cordyceps militaris soybean and preparation method thereof | |
CN105901697A (en) | Fungal extract probiotic functional food | |
CN106387923A (en) | Soluble dietary fibers rich in galactomannan and preparation method of soluble dietary fibers | |
CN105725144A (en) | Bee pollen with high wall-breaking rate and preparation method thereof | |
CN106172675A (en) | A kind of Salicornia Bigelovii Torr. dietary-fiber biscuit and preparation method thereof | |
CN105831773A (en) | Functional food of compound fungal polysaccharide and composition | |
CN105053602A (en) | Mulberry pomace chicken feed and preparation method thereof | |
CN106954775A (en) | A kind of method that utilization microorganism sterile fermentation produces olive tea vinegar drink | |
CN107130006A (en) | The fermentation process and its active product of high activity gumbo polysaccharide | |
CN105768106A (en) | Liver nutrition improving functional food | |
CN106108033A (en) | A kind of preparation method of Entermorpha dietary fiber | |
CN105707803A (en) | High-biological-activity bee pollen and preparation method thereof | |
CN107574124B (en) | The method that Irpex lacteus prepares active dietary fiber with schizophyllum commune fermentation | |
CN105031289A (en) | Dendrobium officinale and lucid ganoderma capsules and preparation method thereof | |
CN117137131A (en) | Polygonatum sibiricum and wolfberry enzyme powder and preparation method thereof | |
CN111743065A (en) | Asparagus beverage and preparation method thereof | |
CN103229666A (en) | Cordyceps militaris peanut and preparing method thereof | |
CN106047643A (en) | Plum blossom vinegar rich in gentian oligosaccharide and fermentation preparation method | |
CN116158490A (en) | A feed additive rich in stachyose extract and Bacillus licheniformis | |
CN105535035A (en) | Inonotus obliquus fermentation culture composition and preparation method thereof | |
CN104928331A (en) | Technology for preparing functional xylo-oligosaccharide by comprehensively utilizing wheat straw |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170201 |