CN106360743A - Extraction method of sea asparagus dietary fiber - Google Patents
Extraction method of sea asparagus dietary fiber Download PDFInfo
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- CN106360743A CN106360743A CN201610860772.1A CN201610860772A CN106360743A CN 106360743 A CN106360743 A CN 106360743A CN 201610860772 A CN201610860772 A CN 201610860772A CN 106360743 A CN106360743 A CN 106360743A
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- dietary fiber
- salicornia bigelovii
- bigelovii torr
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to extraction of dietary fiber, in particular to an extraction method of sea asparagus dietary fiber. The extraction method comprises the following steps of S1, cleaning sea asparagus, drying, smashing, sieving and degreasing; S2, quickly extracting and separating to obtain filter liquor and a filter residue; S3, adding low-fat milk powder and saccharose into the filter liquor, homogenizing and sterilizing to obtain a culture solution; S4, inoculating a mixed culture into the culture solution, and culturing to obtain a fermentation agent; S5, adjusting pH of the filter liquor, then adding complex enzyme, and obtaining enzymatic hydrolysate through enzymolysis; S6, enzyme denaturing and cooling the enzymatic hydrolysate, inoculating the fermentation agent, culturing, and centrifugally separating to obtain filter liquor and a filter residue; S7, washing the filter residue with water, and drying to obtain insoluble dietary fiber; adding ethyl alcohol into the filter liquor, collecting sediments, and drying to obtain water-soluble dietary fiber; S8, mixing the water-soluble dietary fiber and the insoluble dietary fiber. According to the extraction method of the sea asparagus dietary fiber provided by the invention, the reaction condition is moderate, other impurities such as protein and starch can be effectively removed, the yield and the purity of the dietary fiber are improved, and the retention ability and the expansibility of the dietary fiber are improved.
Description
Technical field
The present invention relates to a kind of dietary fiber, more particularly, to a kind of extracting method of Salicornia Bigelovii Torr. dietary fiber.
Background technology
The Carnified euhalophyte of no base of leaf that Salicornia Bigelovii Torr. (salicornia) belongs to for Chenopodiaceae salicornia europaeal, the tolerance of salinity is high,
It is commonly called as marine borer grass, salicornia europaeal, Salicornia Bigelovii Torr. or extra large bean, be more common in lick, seabeach is taken up in salt lake.Recently as both at home and abroad to sea
Deepening continuously of Germinatus Phragmitiss research, has separately obtained polysaccharide, chromocor compound, biogenic salt, chlorophyll etc. multiple from Salicornia Bigelovii Torr.
There is the active component of medical value and health value.Show that chromocor compound has vessel softening, blood sugar lowering, fall by research
Blood fat, antioxidation, removing interior free yl, defying age, resisting pathogenic microbes, infection, anti-inflammatory, antiulcer, antiallergic, rush
Enter the different physiological roles such as tissue regeneration；Polysaccharide has antitumor, antiviral, antiulcer, radioprotective, strengthens immunity, resisting fatigue
The effects such as.
Dietary fiber is described as the seventh-largest nutrient of the mankind, has important function to maintaining human health diet.In recent years
Carry out the raising with people's living standard, diet structure has a very large change, dietary fiber is as a kind of functional food
Base material, increasingly receives significant attention；Dietary fiber divides according to dissolubility and can be divided into water soluble dietary fiber (sdf) and insoluble
Property dietary fiber (idf)；Water soluble dietary fiber (sdf) is the class meals that a class may be dissolved in 100 DEG C of water of ph6～7
Stored substance in fiber, predominantly plant cell and secretions, additionally include microbial polysaccharide and synthesis polysaccharide, its composition
A mainly class gelatin substance and glucide.Sdf can participate in and affect the multiple metabolism in human body, such as lipid metabolism, carbon water
Compound metabolism；Stomach satiety can be increased, reduce food intake dose, there is the effect of prevention obesity.Insoluble meals are fine
Dimension (idf) is the insoluble class dietary fiber in 100 DEG C of water of ph6～7 of a class, and its composition is cellulose, half fiber
Element, lignin, Pectin, proto and shitosan etc..Idf can increase stomach satiety, contributes to enterokinesia, can change as dredging constipation
Solution calculus, loses weight, prevents the functional food of intestinal cancer.
Extract dietary fiber at present from Salicornia Bigelovii Torr., typically extracted using the method that alkaline process or alkaline process are combined with enzyme process,
But using alkaline process due to immersion repeatedly and frequently heat treatment, retention ability and the expansive force of diet fiber product can be reduced,
In addition the alkalies cognition discharged causes environmental pollution；Use Enzymatic Extraction dietary fiber, process time is long；And said method is all
A problem is that gained dietary fiber low yield, purity is low.It is therefore desirable to it is fine to set up a kind of extraction Salicornia Bigelovii Torr. meals
The new method of dimension.
Content of the invention
The technical problem to be solved is: in view of the shortcomings of the prior art, provides a kind of Salicornia Bigelovii Torr. meals
The extracting method of fiber, this method can improve extraction ratio and the purity of dietary fiber, improves retention ability and the expansive force of dietary fiber.
For solving above-mentioned technical problem, the technical scheme is that
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, described extracting method comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder；
S2. defat Salicornia Bigelovii Torr. dry powder in s1 is taken to add volumetric concentration for 5～% according to solid-liquid ratio 1:5～1:10, mixing is all
Carry out homogenate extraction after even, separate and obtain filtering residue and filtrate；
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 1～3% and 0.5～1% sucrose, after mixing well
Sterilize at 100～110 DEG C 3～5min, obtains culture fluid；
S4. take the culture fluid in s3, then lactic acid bacteria, Hypocrea virens and Schizophyllum commune Franch． are inoculated in culture fluid, 22～
At 37 DEG C, culture 4～8h obtains leaven；
S5. take the filtering residue that s2 obtains, adjust ph to 4～5.5, then add the compound of described filtering residue weight 0.1～0.5%
Enzyme, obtains enzymolysis solution through enzymolysis；
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, the leaven of inoculation s4 preparation, then at 25～37 DEG C
Lower culture 12～36h, then stops fermentation, is centrifugally separating to obtain filtrate and filtering residue；
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber；
Add the ethanol that 5～8 times of volumetric concentrations of filtrate volume are 65～85%, stirring, standing, precipitating in filtrate, collect precipitation, be dried
Obtain sea asparagus water-solubility dietary fiber；
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
As a kind of improved technical scheme, the extraction time 1～3min of described homogenate extraction, Extracting temperature 50～60
DEG C, extract voltage 165～220v.
As a kind of improved technical scheme, in step s4 the inoculum concentration of mixed bacteria be culture fluid weight 1.5～
3%.
As a kind of improved technical scheme, in step s4, mixed bacteria is by lactic acid bacteria, Hypocrea virens, sturdy wheat stricture of vagina born of the same parents
Bacterium and Schizophyllum commune Franch． mix according to mass ratio 0.5～1:0.5～1.2:0.4～0.7:0.3～0.7 ratio.
As a kind of improved technical scheme, in step s5, compound enzyme includes protease and a～amylase, and described albumen
Enzyme and a～amylase mix according to the ratio of 0.5～1.2:0.4～0.7.
As a kind of improved technical scheme, in step s6, the inoculum concentration of leaven is the 2～4% of enzymolysis solution weight.
As a kind of preferred technical scheme, step s5 is digested using ultrasonic wave added mode, ultrasonic power 300～600w,
Ultrasonic time 5～15min.
After employing technique scheme, the invention has the beneficial effects as follows:
Compared with prior art, the present invention mainly has the advantage that
(1) present invention, with Salicornia Bigelovii Torr. as raw material, carries out dietary fiber extraction, takes full advantage of Salicornia Bigelovii Torr. active component content
Feature abundant, that comprehensive nutrient is high, has established technical foundation for exploitation dietary fiber.
(2) using volumetric concentration, the ethanol for 5%-15% carries out homogenate extraction to Salicornia Bigelovii Torr., can make flavone in Salicornia Bigelovii Torr.
Compound isoreactivity composition fully extracts, and as culture fluid, prepared by inoculation strain for the filtrate then being obtained with after homogenate extraction
Leaven；Again process is carried out to the filtering residue of homogenate extraction using ultrasonic wave added enzymatic isolation method and obtain enzymolysis solution, this process is using compound
Enzyme can remove the starch in Salicornia Bigelovii Torr., also protein is effectively degraded simultaneously, improves the purity of dietary fiber；Then again
Leaven is inoculated in enzymolysis solution by fermentation, because lactic acid bacteria produces substantial amounts of organic acid in sweat, fiber can be made
The glucosides bond fission of element, reduces the macromolecular polymeric degree of dietary fiber, improves the content of water soluble dietary fiber；Green wood
Mycete is combined the Secretory cycle that sturdy wheat stricture of vagina born of the same parents bacterium fermentation can extend cellulase, contributes to cellulose and fully degrades；In addition split
Gill fungus also can secrete substantial amounts of cellulase and hemicellulase in fermentation, and three's synergic fermentation reduces cellulose and half fibre
Dimension cellulose content, and also improve the molten of Salicornia Bigelovii Torr. dietary fiber while the other active components in retaining Salicornia Bigelovii Torr. are active
Swollen property；
(3) dietary fiber that the present invention is extracted with Salicornia Bigelovii Torr. for raw material, the method pair being combined with fermentation method using enzyme process
Dodge the filtrate after carrying and filtering residue is processed respectively, substantially increase dietary fiber yield and purity, further improve dietary fiber simultaneously
Physicochemical property, this research dietary fiber yield is up to 45.6%, and purity is 92.8%；The retention ability of gained dietary fiber up to
12.16g/g, expansive force is up to 16.8ml/g；Reaction condition is gentle, and dietary fiber loss is few.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
Example 1
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder；
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add, according to solid-liquid ratio 1:5, the ethanol that volumetric concentration is 5%, mixing is all
Carry out homogenate extraction (extraction time 1min, 50 DEG C of Extracting temperature extract voltage 165v) after even, separate and obtain filtering residue and filtrate；
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 1.5% and 0.5% sucrose, after mixing well
Sterilize at 100 DEG C 3min, obtains culture fluid；
S4. take the culture fluid in s3, then by the 1.5% of culture fluid weight mixed bacteria (lactic acid bacteria, Trichoderma viride
Bacterium, sturdy wheat stricture of vagina born of the same parents bacterium and Schizophyllum commune Franch． mix according to the ratio of 0.6:0.7:0.5:0.3) it is inoculated in culture fluid, train at 23 DEG C
Foster 5h obtains leaven；
S5. take the filtering residue that s2 obtains, adjust ph to 4, then add compound enzyme (protease and a of described filtering residue weight 0.1%
～amylase is mixed in the ratio of 0.7:0.4), obtain through ultrasonic wave added enzymolysis (ultrasonic power 360w, ultrasonic time 5min)
Enzymolysis solution；
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
The 2% of liquid weight), then cultivate 24h at 28 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue；
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber；
Add the ethanol that 6 times of volumetric concentrations of filtrate volume are 65%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber；
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 41.9%, and purity is 89.3%, and gained meals are fine
Maintain waterpower 10.2g/g, expansive force 14.2ml/g.
Example 2
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder；
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add, according to solid-liquid ratio 1:8, the ethanol that volumetric concentration is 8%, mixing is all
Carry out homogenate extraction (extraction time 1.5min, 52 DEG C of Extracting temperature extract voltage 170v) after even, separate and obtain filtering residue and filter
Liquid；
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 1.8% and 0.7% sucrose, after mixing well
Sterilize at 108 DEG C 3min, obtains culture fluid；
S4. take the culture fluid in s3, then by the 1.7% of culture fluid weight mixed bacteria (lactic acid bacteria, Hypocrea virens
Mix according to the ratio of 0.8:0.7:0.6:0.5 with Schizophyllum commune Franch．) it is inoculated in culture fluid, at 25 DEG C, culture 6h obtains leaven；
S5. take the filtering residue that s2 obtains, adjust ph to 4.5, then add filtering residue weight 0.2% compound enzyme (protease and a～
Amylase is mixed in the ratio of 1:0.7), digested through ultrasonic wave added enzymolysis (ultrasonic power 400w, ultrasonic time 13min)
Liquid；
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
The 2.5% of liquid weight), then cultivate 28h at 30 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue；
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber；
Add the ethanol that 7 times of volumetric concentrations of filtrate volume are 70%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber；
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 42.8%, and purity is 89.9%, and gained meals are fine
Maintain waterpower 10.8g/g, expansive force 14.9ml/g.
Example 3
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder；
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add the ethanol that volumetric concentration is 10%, mixing according to solid-liquid ratio 1:10
Carry out homogenate extraction (extraction time 1.8min, 55 DEG C of Extracting temperature extract voltage 180v) after uniformly, separate and obtain filtering residue and filter
Liquid；
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 2% and 0.5% sucrose, 110 after mixing well
Sterilize at DEG C 3min, obtains culture fluid；
S4. take the culture fluid in s3, then that the mixed bacteria of culture fluid weight 2% is (lactic acid bacteria, Hypocrea virens, thick
Strong wheat stricture of vagina born of the same parents bacterium and Schizophyllum commune Franch． mix according to the ratio of 1:1:0.4:0.7) it is inoculated in culture fluid, at 28 DEG C, culture 7h is sent out
Ferment agent；
S5. take the filtering residue that s2 obtains, adjust ph to 4.8, then add filtering residue weight 0.3% compound enzyme (protease and a～
Amylase is mixed in the ratio of 0.7:0.6), obtain enzyme through ultrasonic wave added enzymolysis (ultrasonic power 450w, ultrasonic time 8min)
Solution liquid；
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
The 3% of liquid weight), then cultivate 30h at 32 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue；
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber；
Add the ethanol that 7 times of volumetric concentrations of filtrate volume are 75%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber；
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 43.9%, and purity is 90.3%, and gained meals are fine
Maintain waterpower 11.2g/g, expansive force 15.2ml/g.
Example 4
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder；
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add the ethanol that volumetric concentration is 10%, mixing according to solid-liquid ratio 1:8
Carry out homogenate extraction (extraction time 2.5min, 60 DEG C of Extracting temperature extract voltage 200v) after uniformly, separate and obtain filtering residue and filter
Liquid；
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 2.5% and 0.8% sucrose, after mixing well
Sterilize at 105 DEG C 4.5min, obtains culture fluid；
S4. take the culture fluid in s3, then by the 2.5% of culture fluid weight mixed bacteria (lactic acid bacteria, Trichoderma viride
Bacterium, sturdy wheat stricture of vagina born of the same parents bacterium and Schizophyllum commune Franch． mix according to the ratio of 1:1:0.7:0.7) it is inoculated in culture fluid, cultivate 5h at 30 DEG C
Obtain leaven；
S5. take the filtering residue that s2 obtains, adjust ph to 5.5, then add compound enzyme (protease and a of filtering residue weight 0.45%
～amylase is mixed in the ratio of 0.5:0.7), obtain through ultrasonic wave added enzymolysis (ultrasonic power 500w, ultrasonic time 12min)
Enzymolysis solution；
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
Liquid weight 3.5%), then cultivate 36h at 35 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue；
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber；
Add the ethanol that 8 times of volumetric concentrations of filtrate volume are 80%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber；
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 45.6%, and purity is 92.8%, and gained meals are fine
Maintain waterpower 12.16g/g, expansive force 16.8ml/g.
Example 5
A kind of extracting method of Salicornia Bigelovii Torr. dietary fiber, comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain
Defat Salicornia Bigelovii Torr. dry powder；
S2. defat Salicornia Bigelovii Torr. dry powder 20g in s1 is taken to add the ethanol that volumetric concentration is 15%, mixing according to solid-liquid ratio 1:10
Carry out homogenate extraction (extraction time 3min, 60 DEG C of Extracting temperature extract voltage 220v) after uniformly, separate and obtain filtering residue and filter
Liquid；
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 3% and 1% sucrose, at 105 DEG C after mixing well
Lower sterilizing 3min, obtains culture fluid；
S4. take the culture fluid in s3, then by the 3% of culture fluid weight mixed bacteria (lactic acid bacteria, Hypocrea virens and
Schizophyllum commune Franch． mixes according to the ratio of 1:1.2:0.7:0.7) it is inoculated in culture fluid, at 28 DEG C, culture 5h obtains leaven；
S5. take the filtering residue that s2 obtains, adjust ph to 5.2, then add filtering residue weight 0.5% compound enzyme (protease and a～
Amylase is mixed in the ratio of 1.2:0.7), obtain enzyme through ultrasonic wave added enzymolysis (ultrasonic power 560w, ultrasonic time 10min)
Solution liquid；
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, (inoculum concentration is enzymolysis for leaven inoculation prepared by s4
Liquid weight 4%), then cultivate 36h at 37 DEG C, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue；
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber；
Add the ethanol that 8 times of volumetric concentrations of filtrate volume are 85%, stirring, standing, precipitating in filtrate, collect precipitation, be dried to obtain sea
Asparagus water-solubility dietary fiber；
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr.
Dietary fiber.
Under this process conditions, the extraction ratio of Salicornia Bigelovii Torr. dietary fiber is 45.2%, and purity is 92.3%, and gained meals are fine
Maintain waterpower 11.85%, expansive force 16.2ml/g.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (7)
1. a kind of extracting method of Salicornia Bigelovii Torr. dietary fiber is it is characterised in that described extracting method comprises the following steps:
S1. pluck Salicornia Bigelovii Torr. fresh leaf, after over cleaning, drying, pulverizing, ethyl acetate backflow ungrease treatment, drain and obtain defat
Salicornia Bigelovii Torr. dry powder；
S2. defat Salicornia Bigelovii Torr. dry powder in s1 is taken to add, according to solid-liquid ratio 1:5～1:10, the ethanol that volumetric concentration is 5%～15%,
Carry out homogenate extraction after mix homogeneously, separate and obtain filtering residue and filtrate；
S3. take the filtrate of s2 gained, add the low fat milk powder of filtrate weight 1～3% and 0.5～1% sucrose, after mixing well
Sterilize at 100～110 DEG C 3～5min, obtains culture fluid；
S4. take the culture fluid in s3, then mixed bacteria is inoculated in culture fluid, culture 4～8h obtains at 22～37 DEG C
Leaven；
S5. take the filtering residue that s2 obtains, adjust ph to 4～5.5, then add the compound enzyme of described filtering residue weight 0.1～0.5%, warp
Cross enzymolysis and obtain enzymolysis solution；
S6. by after the enzymolysis solution enzyme denaturing of s5 gained, cooling treatment, inoculate the leaven of s4 preparation, then train at 25～37 DEG C
Support 12～36h, then stop fermentation, be centrifugally separating to obtain filtrate and filtering residue；
S7. the filtering residue that s6 obtains and filtrate are taken, filtering residue is washed to neutrality, is dried to obtain Salicornia Bigelovii Torr. insoluble dietary fiber；Filtrate
Middle addition 5～8 times of volumetric concentrations of filtrate volume are 65～85% ethanol, and stirring, standing, precipitating are collected precipitation, be dried to obtain
Sea asparagus water-solubility dietary fiber；
S8. the Salicornia Bigelovii Torr. insoluble dietary fiber in s7 and sea asparagus water-solubility dietary fiber are mixed, obtain Salicornia Bigelovii Torr. meals
Fiber.
2. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: described homogenate extraction
Extraction time 1～3min, 50～60 DEG C of Extracting temperature, extract voltage 165～220v.
3. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: in step s4 mix
The inoculum concentration of strain is the 1.5～3% of culture fluid weight.
4. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: in step s4 mix
Strain by lactic acid bacteria, Hypocrea virens, sturdy wheat stricture of vagina born of the same parents bacterium and Schizophyllum commune Franch． according to mass ratio 0.5～1:0.5～1.2:0.4～
0.7:0.3～0.7 ratio mixes.
5. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: compound in step s5
Enzyme includes protease and a～amylase, and described protease and a～amylase mix according to the ratio of 0.5～1.2:0.4～0.7
Close.
6. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: step s5 is using super
Sound supplementary mode digests, ultrasonic power 300～600w, ultrasonic time 5～15min.
7. a kind of Salicornia Bigelovii Torr. dietary fiber according to claim 1 extracting method it is characterised in that: in step s6 ferment
The inoculum concentration of agent is the 2～4% of enzymolysis solution weight.
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