CN105230321B - A kind of cuttage breeding method of borneol type burmannii tree - Google Patents
A kind of cuttage breeding method of borneol type burmannii tree Download PDFInfo
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- CN105230321B CN105230321B CN201510765630.2A CN201510765630A CN105230321B CN 105230321 B CN105230321 B CN 105230321B CN 201510765630 A CN201510765630 A CN 201510765630A CN 105230321 B CN105230321 B CN 105230321B
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- cutting
- cuttage
- breeding method
- burmannii
- borneol
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- DTGKSKDOIYIVQL-WEDXCCLWSA-N (+)-borneol Chemical compound C1C[C@@]2(C)[C@@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-WEDXCCLWSA-N 0.000 title claims abstract description 51
- REPVLJRCJUVQFA-UHFFFAOYSA-N (-)-isopinocampheol Natural products C1C(O)C(C)C2C(C)(C)C1C2 REPVLJRCJUVQFA-UHFFFAOYSA-N 0.000 title claims abstract description 47
- CKDOCTFBFTVPSN-UHFFFAOYSA-N borneol Natural products C1CC2(C)C(C)CC1C2(C)C CKDOCTFBFTVPSN-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 229940116229 borneol Drugs 0.000 title claims abstract description 47
- DTGKSKDOIYIVQL-UHFFFAOYSA-N dl-isoborneol Natural products C1CC2(C)C(O)CC1C2(C)C DTGKSKDOIYIVQL-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 238000009395 breeding Methods 0.000 title claims abstract description 30
- 238000005520 cutting process Methods 0.000 claims abstract description 109
- 241001597008 Nomeidae Species 0.000 claims abstract description 18
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 73
- 239000000243 solution Substances 0.000 claims description 30
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 20
- 239000007921 spray Substances 0.000 claims description 18
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 16
- 239000011449 brick Substances 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000004563 wettable powder Substances 0.000 claims description 14
- 230000003020 moisturizing effect Effects 0.000 claims description 11
- 239000008188 pellet Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 9
- 239000000645 desinfectant Substances 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 7
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 7
- 241001330002 Bambuseae Species 0.000 claims description 7
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 7
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 7
- 239000011425 bamboo Substances 0.000 claims description 7
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 7
- 229910052709 silver Inorganic materials 0.000 claims description 7
- 239000004332 silver Substances 0.000 claims description 7
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 7
- 229940033663 thimerosal Drugs 0.000 claims description 7
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 7
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000013522 chelant Substances 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 230000000855 fungicidal effect Effects 0.000 claims description 4
- 239000000417 fungicide Substances 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 3
- 230000000996 additive effect Effects 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- -1 silver ions Chemical class 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 9
- 230000013011 mating Effects 0.000 abstract description 3
- 230000006698 induction Effects 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- DTGKSKDOIYIVQL-NQMVMOMDSA-N (+)-Borneol Natural products C1C[C@]2(C)[C@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-NQMVMOMDSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 230000003750 conditioning effect Effects 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000005648 plant growth regulator Substances 0.000 description 3
- 239000002985 plastic film Substances 0.000 description 3
- 229920006255 plastic film Polymers 0.000 description 3
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 3
- 241000723347 Cinnamomum Species 0.000 description 2
- 240000005636 Dryobalanops aromatica Species 0.000 description 2
- 241000218195 Lauraceae Species 0.000 description 2
- ZSILVJLXKHGNPL-UHFFFAOYSA-L S(=S)(=O)([O-])[O-].[Ag+2] Chemical compound S(=S)(=O)([O-])[O-].[Ag+2] ZSILVJLXKHGNPL-UHFFFAOYSA-L 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 230000000873 masking effect Effects 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 244000037185 Cinnamomum burmannii Species 0.000 description 1
- 235000014487 Cinnamomum burmannii Nutrition 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 240000007182 Ochroma pyramidale Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 229960000846 camphor Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of cuttage breeding method of borneol type burmannii tree, belong to tree cuttage mating system technical field, technical points comprise the following steps：(1) cutting is gathered；(2) cutting is handled：The semi-lignified new life branch of collection is trimmed to the cutting that length is 5~8cm, base portion is cut into inclined-plane, tied using every 50~60 root cutting as a bundle, the lower end of cutting be soaked in bioactivity derivant solution 8~12 it is small when, it is spare；(3) slotting machine prepares；(4) cuttage；A kind of the present invention is intended to provide cuttage breeding method for the borneol type burmannii tree that survival rate is high, the cuttage cycle is short；Cutting propagation for borneol type burmannii tree.
Description
Technical field
The present invention relates to a kind of trees mating system, more specifically, more particularly to a kind of cuttage of borneol type burmannii tree
Mating system.
Background technology
Borneol type burmannii tree, that is, Borneolum tree (Dryobalanops aromatica) belongs to Lauraceae (Lauraceae) Cinnamomum
(Cinnamomum) aiphyllium, formalness are no different with burmannii plant (Cinnamomum burmannii B1.), are one
Physiology or chemical type burmannii rich in d-Bomeol.Contained Borneolum, scientific name " natural d-borneol " in its branches and leaves, are a kind of
Fine perfumery, and a kind of rare rare medicinal material, are widely used in the industries such as medicine, cosmetics, spices, because it can effectively press down
The generation of system or a variety of degenerative disorders such as epidemic prevention system degradation and angiocardiopathy, clinically using extremely wide,
It is the main component of a variety of famous-brand and high-quality Chinese patent drugs.The borneo camphor tree tree root for originating in Indonesia Sumatra archipelago is natural d-borneol
The main source of early stage, over nearly more than 100 years, the natural d-borneol in China relies primarily on import to meet needs, due to locality
Over-exploitation for a long time, natural resources destroy seriously, and local government, which has forbidden cutting down trees, in addition takes brain, thus, natural dextrorotation
Borneol product is in great shortage, more and more concerned.
The effectively product assay such as d-Bomeol is low in common burmannii tree branches and leaves, is effectively produced in the borneol type burmannii branches and leaves of selection and breeding
Thing content is high, can keep merit using cutting propagation mode (vegetative propagation), and quickly breeding goes out breeding nursery stock.With above-mentioned
It is to solve medicine source important channel in short supply that mode, which establishes raw material forestbase,.Therefore, it is necessary to research and develop a kind of survival rate height, skewer
Insert the cuttage breeding method of cycle short borneol type burmannii tree.
The content of the invention
It is an object of the invention in view of the above shortcomings of the prior art, there is provided a kind of survival rate is high, the cuttage cycle is short
The cuttage breeding method of borneol type burmannii tree.
The technical proposal of the invention is realized in this way：A kind of cuttage breeding method of borneol type burmannii tree, this method bag
Include following step：
(1) cutting is gathered：, will half wood during borneol type burmannii tree new life branch semi-lignified annual October to December
Matter new life branch is adopted down, spare；
(2) cutting is handled：The semi-lignified new life branch of collection is trimmed to the cutting that length is 5~8cm, base portion is cut into
Inclined-plane, is tied using every 50~60 root cutting as a bundle, and it is small that the lower end of cutting is soaked in bioactivity derivant solution 8~12
When, it is spare；
(3) slotting machine prepares：Cutting bed is located in cool canopy, surrounding common brick brick work, the group on the non-ground top layer of filling in slotting machine
As cutting medium, 10~15cm of thickness, flattens and pours permeable rear bed surface thimerosal spray disinfectant granular yellow mud, spare；
(4) cuttage：The small bamboo spike consistent with cutting with thickness delineates shallow ridges on slotting machine, after by the cutting handled well by strain
Away from being inserted into for 3~5cm in ditch, the deep 3~5cm of soil is inserted into, cuttage is delineated line by line by line-spacing 5cm, water of drenching after being plugged, and spray and kill
Once, lid diaphragm seal moisturizing, humid control is capped one layer of obscurity to microbial inoculum in the range of 70%~90% outside film in film
For 70~75% sunshade net, checked once every 10~15 days；Temperature automatically controlled moisture control device is selected to carry out certainly shady temperature of shed
Dynamic control control management, throws off film for 110 days~130 days, and upper bag of transplanting seedlings after 140 days~160 days cultivates container seedling.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, step (2) the cutting processing is specially：It will adopt
The semi-lignified new life branch of collection is trimmed to the cutting that length is 5~8cm, retains cutting top vane 1~2, base portion is cut into
40 °~60 ° inclined-planes, are tied using every 50~60 root cutting as a bundle, and the lower end of cutting is soaked in 8 in bioactivity derivant solution
~12 it is small when, it is spare.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, bioactivity derivant is molten described in step (2)
Liquid is methyl α-naphthyl acetate, draws the mixed solution of diindyl butyric acid and silver nitrate, and its preparation method is：First by methyl α-naphthyl acetate and the quality for drawing diindyl butyric acid
Than the mixed solution for being 100~200mg/L for 1~3 ﹕, 1 compound concentrations, silver nitrate is then added according to the total amount of mixed solution,
The additive amount of silver nitrate adds 1~10mg for every liter of mixed solution, up to bioactivity derivant solution.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, the silver nitrate can use silver ion containing equivalent
The silver ion chelate of concentration is replaced.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, step (3) the slotting machine preparation is specially：Cuttage
Bed is located in cool canopy, surrounding common brick brick work, and the pellet shape yellow mud on the non-ground top layer of filling is as cutting medium, thickness in slotting machine
10~15cm, flattens and pours permeable rear bed surface thimerosal spray disinfectant, then stand 5~10 days with covered rearing with plastic film,
Film was thrown off using first 1~2 day, is poured permeable spare.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, the length of cutting bed described in step (3) for 3~
5m, width are 1~1.5m, and depth is 20~40cm.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, the diameter of pellet shape yellow mud described in step (3)
For 2~5mm.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, thimerosal described in step (3) is 1000 times
50% carbendazol wettable powder solution.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, fungicide described in step (4) is 2500 times
70% thiophanate methyl wettable powder agent solution.
In a kind of cuttage breeding method of above-mentioned borneol type burmannii tree, temperature requirement control in cool canopy described in step (4)
System is less than 30 DEG C.
After the present invention uses the above method, compared with prior art, have the advantages that following：
(1) cutting plantation and survival rate are widely different because plant variety is different, and particularly different plants are preferably selected
Variety classes and the plant growth regulator of concentration (plant hormone) or its combination carry out cutting processing, and the present invention is used to have and carried
Rise the chemical induction (Ag of bioactivity+) mixed with two plant growth regulators methyl α-naphthyl acetates (NAA) with drawing diindyl butyric acid (IBA)
The bioactivity derivant solution immersion treatment cutting formed, can be obviously promoted borneol type burmannii tree cutting base portion callus
Formed, be conducive to differentiate healthy and strong root system, effectively increase rooting of cuttings rate and its survival rate；It is demonstrated experimentally that using silver nitrate
Or silver ion (the Ag of equivalent concentration+) formed bioactivity derivant solution is mixed with two plant growth regulators, have
There is same effect.
(2) traditional cutting medium selects yellow mud, river sand or perlite etc. to be used after being mixed with certain proportion, cuttage of the present invention
Matrix selects the pellet shape yellow mud on non-ground top layer, and the debris containing miscellaneous bacteria and vegetable seeds etc. is few, can reach and reduces weeds and disease pest
The purpose that evil occurs, is conducive to improve cuttage survival rate；The pellet shape yellow mud of 100%1~5mm diameters is selected to make cutting medium,
With good water-retaining property, make the contiguity performance of root system and soil good, and cutting angular cut and cutting medium contact surface are larger, skewer
Insert and be easier to survive；
(3) conventional management mode is to adjust the temperature of slotting machine and wet by manual or automatic control device after the completion of cuttage
Degree, but repeated multiple times water spray easily leads to soil hardening influence cuttage quality, and the present invention is complete using masking film after the completion of cuttage
Hermetically sealed training method moistening effect is notable, can obviously reduce water spray number, at the same cutting be affected by the external environment it is small, hence it is evident that subtract
The chance of few secondary microbiological contamination, the management workload such as water spray spray significantly reduces after cuttage, and survival rate greatly improves；
(4) it is additional after cutting bed masking film according to the relatively low characteristic of borneol type burmannii tree early development light saturation point
One layer of obscurity of lid is 70~75% sunshade net, can be improved to avoid strong sunlight directly according to the injury to cutting blade and rudiment
Cuttage survival rate.Therefore, cottage method of the invention can successfully breed borneol type burmannii sapling, and cuttage survival rate can reach 90%
More than, 96.3% is can reach, seedling cost is significantly reduced and meets the needs of market.
Embodiment
With reference to specific embodiment, the present invention is described in further detail, but does not form to any of the present invention
Limitation.
A kind of cuttage breeding method of borneol type burmannii tree of the present invention, this method comprise the following steps：
(1) cutting is gathered：, will half wood during borneol type burmannii tree new life branch semi-lignified annual October to December
Matter new life branch is adopted down, spare；
(2) cutting is handled：The semi-lignified new life branch of collection is trimmed to the cutting that length is 5~8cm, retains cutting
Top vane 1~2, base portion are cut into 40 °~60 ° inclined-planes, are tied using every 50~60 root cutting as a bundle, the lower end immersion of cutting
In bioactivity derivant solution 8~12 it is small when, it is spare；Wherein, the bioactivity derivant solution is methyl α-naphthyl acetate, draws diindyl
The mixed solution of butyric acid and silver nitrate, its preparation method are：It is first 1 with the mass ratio for drawing diindyl butyric acid (IBA) by methyl α-naphthyl acetate (NAA)
1 compound concentrations of~3 ﹕ are the mixed solution of 100~200mg/L, then add silver nitrate, silver nitrate according to the total amount of mixed solution
Additive amount add 1~10mg for every liter of mixed solution, up to bioactivity derivant solution.Silver nitrate (the AgNO3) can
With using the silver ion (Ag of equivalent molar concentration (μm ol/L)+) chelate.
(3) slotting machine prepares：Length is 3~5m, and width is 1~1.5m, and depth is that the cutting bed of 20~40cm is located at cool canopy
Interior, surrounding common brick brick work, the pellet shape yellow mud of non-ground top layer and a diameter of 2~5mm is filled in slotting machine as cutting medium,
10~15cm of thickness, flattens and pours permeable rear bed surface thimerosal spray disinfectant, then stand 5~10 with covered rearing with plastic film
My god, throw off film within 1~2 day before use, pour permeable spare；The thimerosal is 1000 times of 50% carbendazol wettable powder
Solution.
(4) cuttage：The small bamboo spike consistent with cutting with thickness delineates shallow ridges on slotting machine, after by the cutting handled well by strain
Away from being inserted into for 3~5cm in ditch, the deep 3~5cm of soil is inserted into, cuttage is delineated line by line by line-spacing 5cm, water of drenching after being plugged, and spray and kill
Once, lid diaphragm seal moisturizing, humid control is capped one layer of obscurity to microbial inoculum in the range of 70%~90% outside film in film
For 70~75% sunshade net, checked once every 10~15 days；Temperature automatically controlled moisture control device is selected to carry out certainly shady temperature of shed
Dynamic control control manages, and temperature requirement control is less than 30 DEG C in cool canopy；Throw off within 110 days~130 days film, 140 days~160 days
After transplant seedlings upper bag cultivate container seedling.The fungicide is 2500 times of 70% thiophanate methyl wettable powder agent solution.
Embodiment 1
A kind of cuttage breeding method of borneol type burmannii tree, this method comprise the following steps：
(1) when annual early October, borneol type burmannii tree new life branch semi-lignified, by semi-lignified new life branch
Adopt down, it is spare；
(2) cutting is handled：The semi-lignified branch of collection is trimmed to the cutting that length is 5~8cm, is retained at the top of cutting
1~2, blade, base portion are cut into 40 °~60 ° inclined-planes, are tied using every 50 root cutting as a bundle, the lower end of cutting is soaked in life respectively
In thing activity inducement agent solution 12 it is small when, test group use bioactivity derivant solution species and concentration it is as follows：1. group：Nitre
Sour silver (AgNO3)1mg/L+NAA 50mg/L+IBA 50mg/L；2. group：Silver nitrate (AgNO3)1mg/L+NAA 100mg/L+IBA
50mg/L；3. group：Silver nitrate (AgNO3)1mg/L+NAA 150mg/L+IBA 50mg/L；4. group：NAA 50mg/L+IBA
50mg/L。
(3) slotting machine prepares：Length is 3m, and width 1m, depth is that the cutting bed of 25cm is located in cool canopy, surrounding common brick
Brick work, the pellet shape yellow mud of non-ground top layer and a diameter of 2~5mm is filled in slotting machine as cutting medium, filling thickness 10cm,
Flatten and pour it is permeable after bed surface with 50% carbendazol wettable powder, 1000 times of liquid spray disinfectants once, then covered with plastic film
Lid processing 8 days, throws off film in 1 day before use, pours permeable spare；
(4) cuttage：The small bamboo spike consistent with cutting with thickness delineates shallow ridges on slotting machine, after by the cutting handled well by strain
Away from being inserted into for 3~5cm in ditch, the deep 3~5cm of soil is inserted into, cuttage is delineated line by line by line-spacing 5cm, water of drenching after being plugged, and spray
Once, lid diaphragm seal moisturizing, film is capped one layer of obscurity to 2500 times of 70% thiophanate methyl wettable powder agent solution outside
For 75% sunshade net, checked once every 10 days, moisturizing of spraying water if necessary.Temperature control is less than 30 DEG C in cool canopy, throws off within 120 days
Film, upper bag of transplanting seedlings after 150 days cultivate container seedling.
The result shows that：Cutting processing is using the chemical induction (Ag with lifting bioactivity+) and two plant growths
The mixed solution of conditioning agent NAA+IBA (1~3 ﹕ 1), can be obviously promoted borneol type burmannii tree cutting base portion calli induction,
Be conducive to differentiate healthy and strong root system, each test group rooting of cuttings rate reaches 1. group：88.5%；2. group：93.3%；3. group：
90.8%；4. group：80.3%.
Embodiment 2
A kind of cuttage breeding method of borneol type burmannii tree, this method comprise the following steps：
(1) when annual late October, borneol type burmannii tree new life branch semi-lignified, by semi-lignified new life branch
Adopt down, it is spare；
(2) cutting is handled：The semi-lignified branch of collection is trimmed to the cutting that length is 5~8cm, is retained at the top of cutting
1~2, blade, base portion are cut into 40 °~60 ° inclined-planes, are tied using every 50 root cutting as a bundle, the lower end of cutting is soaked in life respectively
In thing activity inducement agent solution 8 it is small when, test group use bioactivity derivant solution species and concentration it is as follows：1. group：Sulphur
Silver thiosulfate Ag2S2O3(silver ion Ag+3 μm of ol/L of chelate)+NAA 50mg/L+IBA 50mg/L；2. group：Silver thiosulfate
Ag2S2O3(Ag+Chelate) 3 μm of ol/L+NAA 100mg/L+IBA 50mg/L；3. group：Silver thiosulfate Ag2S2O3(Ag+Chelating
Thing) 3 μm of ol/L+NAA 150mg/L+IBA 50mg/L；4. group：NAA 50mg/L+IBA 50mg/L.3 μm of ol/ of silver thiosulfate
L silver ions and silver nitrate (AgNO3) 2mg/L is suitable.
(3) slotting machine prepares：Length is 4m, width 1.5m, and depth is that the cutting bed of 40cm is located in cool canopy, and surrounding is with red
Brick brick work, the interior pellet shape yellow mud for filling non-ground top layer and a diameter of 2~5mm of slotting machine is as cutting medium, filling thickness
15cm, flatten and pour it is permeable after bed surface with 50% carbendazol wettable powder, 1000 times of liquid spray disinfectants once, then use plastics
Film covering treatment 6 days, throws off film in 2 days before use, pours permeable spare；
(4) cuttage：The small bamboo spike consistent with cutting with thickness delineates shallow ridges on slotting machine, after by the cutting handled well by strain
Away from being inserted into for 3~5cm in ditch, the deep 3~5cm of soil is inserted into, cuttage is delineated line by line by line-spacing 5cm, water of drenching after being plugged, and spray
Once, lid diaphragm seal moisturizing, film is capped one layer of obscurity to 2500 times of 70% thiophanate methyl wettable powder agent solution outside
For 75% sunshade net, checked once every 15 days, moisturizing of spraying water if necessary.Temperature control is less than 30 DEG C in cool canopy, throws off within 110 days
Film, upper bag of transplanting seedlings after 140 days cultivate container seedling.
The result shows that：Cutting processing is using the chemical induction (Ag with lifting bioactivity+) and two plant growths
The mixed solution of conditioning agent NAA+IBA (1~3 ﹕ 1), can be obviously promoted borneol type burmannii tree cutting base portion calli induction,
Be conducive to differentiate healthy and strong root system, each test group rooting of cuttings rate reaches 1. group：91.5%；2. group：96.3%；3. group：
92.8%；4. group：82.9%.
Embodiment 3
A kind of cuttage breeding method of borneol type burmannii tree, this method comprise the following steps：
(1) when annual mid-November, borneol type burmannii tree new life branch semi-lignified, by semi-lignified new life branch
Adopt down, it is spare；
(2) cutting is handled：The semi-lignified branch of collection is trimmed to the cutting that length is 5~8cm, is retained at the top of cutting
1~2, blade, base portion are cut into 40 °~60 ° inclined-planes, are tied using every 60 root cutting as a bundle, the lower end of cutting is soaked in life respectively
In thing activity inducement agent solution 10 it is small when, test group use bioactivity derivant solution species and concentration it is as follows：1. group：Nitre
Sour silver (AgNO3)5mg/L+NAA 50mg/L+IBA 50mg/L；2. group：Silver nitrate (AgNO3)5mg/L+NAA 100mg/L+IBA
50mg/L；3. group：Silver nitrate (AgNO3)5mg/L+NAA 150mg/L+IBA 50mg/L；4. group：NAA 50mg/L+IBA
50mg/L。
(3) slotting machine prepares：Length is 5m, width 1.5m, and depth is that the cutting bed of 20cm is located in cool canopy, and surrounding is with red
Brick brick work, the interior pellet shape yellow mud for filling non-ground top layer and a diameter of 2~5mm of slotting machine is as cutting medium, filling thickness
12cm, flatten and pour it is permeable after bed surface with 50% carbendazol wettable powder, 1000 times of liquid spray disinfectants once, then use plastics
Film covering treatment 10 days, throws off film in 1 day before use, pours permeable spare；
(4) cuttage：The small bamboo spike consistent with cutting with thickness delineates shallow ridges on slotting machine, after by the cutting handled well by strain
Away from being inserted into for 3~5cm in ditch, the deep 3~5cm of soil is inserted into, cuttage is delineated line by line by line-spacing 5cm, water of drenching after being plugged, and spray
Once, lid diaphragm seal moisturizing, film is capped one layer of obscurity to 2500 times of 70% thiophanate methyl wettable powder agent solution outside
For 75% sunshade net, checked once every 10 days, moisturizing of spraying water if necessary.Shady temperature of shed is preferably controlled less than 30 DEG C, is taken off within 130 days
Film is removed, upper bag of transplanting seedlings after 160 days cultivates container seedling.
The result shows that：Cutting processing is using the chemical induction (Ag with lifting bioactivity+) and two plant growths
The mixed solution of conditioning agent NAA+IBA (1~3 ﹕ 1), can be obviously promoted borneol type burmannii tree cutting base portion calli induction,
Be conducive to differentiate healthy and strong root system, each test group rooting of cuttings rate reaches 1. group：92.3%；2. group：95.8%；3. group：
91.6%；4. group：83.0%.
Embodiment 4
A kind of cuttage breeding method of borneol type burmannii tree, this method comprise the following steps：
(1) when annual early December, borneol type burmannii tree new life branch semi-lignified, by semi-lignified new life branch
Adopt down, it is spare；
(2) cutting is handled：The semi-lignified branch of collection is trimmed to the cutting that length is 5~8cm, is retained at the top of cutting
1~2, blade, base portion are cut into 40 °~60 ° inclined-planes, are tied using every 55 root cutting as a bundle, the lower end of cutting is soaked in life respectively
In thing activity inducement agent solution 9 it is small when, test group use bioactivity derivant solution species and concentration it is as follows：1. group：Nitre
Sour silver (AgNO3)10mg/L+NAA 50mg/L+IBA 50mg/L；2. group：Silver nitrate (AgNO3)10mg/L+NAA100mg/L+
IBA 50mg/L；3. group：Silver nitrate (AgNO3)10mg/L+NAA 150mg/L+IBA 50mg/L；4. group：NAA 50mg/L+
IBA 50mg/L。
(3) slotting machine prepares：Length is 3.5m, width 1m, and depth is that the cutting bed of 30cm is located in cool canopy, and surrounding is with red
Brick brick work, the interior pellet shape yellow mud for filling non-ground top layer and a diameter of 2~5mm of slotting machine is as cutting medium, filling thickness
14cm, flatten and pour it is permeable after bed surface with 50% carbendazol wettable powder, 1000 times of liquid spray disinfectants once, then use plastics
Film covering treatment 5 days, throws off film in 2 days before use, pours permeable spare；
(4) cuttage：The small bamboo spike consistent with cutting with thickness delineates shallow ridges on slotting machine, after by the cutting handled well by strain
Away from being inserted into for 3~5cm in ditch, the deep 3~5cm of soil is inserted into, cuttage is delineated line by line by line-spacing 5cm, water of drenching after being plugged, and spray
Once, lid diaphragm seal moisturizing, film is capped one layer of obscurity to 2500 times of 70% thiophanate methyl wettable powder agent solution outside
For 75% sunshade net, checked once every 15 days, moisturizing of spraying water if necessary.Shady temperature of shed is preferably controlled less than 30 DEG C, is taken off within 120 days
Film is removed, upper bag of transplanting seedlings after 150 days cultivates container seedling.
The result shows that：Cutting processing is using the chemical induction (Ag with lifting bioactivity+) and two plant growths
The mixed solution of conditioning agent NAA+IBA (1~3 ﹕ 1), can be obviously promoted borneol type burmannii tree cutting base portion calli induction,
Be conducive to differentiate healthy and strong root system, each test group rooting of cuttings rate reaches 1. group：90.1%；2. group：93.8%；3. group：
90.6%；4. group：81.1%.
Embodiment provided above is the better embodiment of the present invention, is only used for the convenient explanation present invention, not to this hair
The limitation of bright work in any form, any those of ordinary skill in the art, if putting forward skill not departing from the present invention
In the range of art feature, using the equivalent embodiment for locally changing or modifying made by disclosed technology contents, and
Without departing from the technical feature content of the present invention, in the range of still falling within the technology of the present invention feature.
Claims (7)
1. a kind of cuttage breeding method of borneol type burmannii tree, it is characterised in that this method comprises the following steps：
(1) cutting is gathered：Annual October to December, borneol type burmannii tree new life branch semi-lignified when, by semi-lignified
Newborn branch is adopted down, spare；
(2) cutting is handled：The semi-lignified new life branch of collection is trimmed to the cutting that length is 5~8cm, base portion is cut into tiltedly
Face, is tied using every 50~60 root cutting as a bundle, the lower end of cutting be soaked in bioactivity derivant solution 8~12 it is small when,
It is spare；The bioactivity derivant solution is methyl α-naphthyl acetate, draws the mixed solution of diindyl butyric acid and silver nitrate, and its preparation method is：
First by methyl α-naphthyl acetate and to draw the mass ratio of diindyl butyric acid be mixed solution that 1~3 ﹕, 1 compound concentrations are 100~200mg/L, then basis
The total amount addition silver nitrate of mixed solution, the additive amount of silver nitrate adds 1~10mg for every liter of mixed solution, up to bioactivity
Derivant solution；
(3) slotting machine prepares：Cutting bed is located in cool canopy, surrounding common brick brick work, the pellet shape on the non-ground top layer of filling in slotting machine
As cutting medium, 10~15cm of thickness, flattens and pours permeable rear bed surface thimerosal spray disinfectant yellow mud, then thin with plastics
Film covering stands 5~10 days, throws off film within 1~2 day before use, pours permeable spare；A diameter of the 1 of the pellet shape yellow mud~
5mm；
(4) cuttage：The small bamboo spike consistent with cutting with thickness delineates shallow ridges on slotting machine, after by the cutting handled well be by spacing in the rows
In 3~5cm insertion ditches, the deep 3~5cm of soil is inserted into, cuttage is delineated line by line by line-spacing 5cm, water of drenching after being plugged, and spraying fungicide
Once, lid diaphragm seal moisturizing, it is 70 that humid control is capped one layer of obscurity in the range of 70%~90%, outside film in film
~75% sunshade net, checked once every 10~15 days；Temperature automatically controlled moisture control device is selected to control shady temperature of shed automatically
System control management, throws off film for 110 days~130 days, and upper bag of transplanting seedlings after 140 days~160 days cultivates container seedling.
A kind of 2. cuttage breeding method of borneol type burmannii tree according to claim 1, it is characterised in that step (2) institute
Stating cutting processing is specially：The semi-lignified new life branch of collection is trimmed to the cutting that length is 5~8cm, retains cutting top
1~2, portion's blade, base portion are cut into 40 °~60 ° inclined-planes, are tied using every 50~60 root cutting as a bundle, the lower end of cutting is soaked in
In bioactivity derivant solution 8~12 it is small when, it is spare.
A kind of 3. cuttage breeding method of borneol type burmannii tree according to claim 1, it is characterised in that the silver nitrate
The silver ion chelate of equivalent concentration of silver ions can be used to replace.
4. the cuttage breeding method of a kind of borneol type burmannii tree according to claim 1, it is characterised in that in step (3)
The length of the cutting bed is 3~5m, and width is 1~1.5m, and depth is 20~40cm.
5. the cuttage breeding method of a kind of borneol type burmannii tree according to claim 1, it is characterised in that in step (3)
The thimerosal is 1000 times of 50% carbendazol wettable powder solution.
6. the cuttage breeding method of a kind of borneol type burmannii tree according to claim 1, it is characterised in that in step (4)
The fungicide is 2500 times of 70% thiophanate methyl wettable powder agent solution.
7. the cuttage breeding method of a kind of borneol type burmannii tree according to claim 1, it is characterised in that in step (4)
Temperature requirement control is less than 30 DEG C in the cool canopy.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263765A (en) * | 2008-05-13 | 2008-09-17 | 湖南省新晃县龙脑开发有限责任公司 | Cirmamonun camphora clone scale fast propagation seedling-breeding method |
| CN101473736A (en) * | 2008-05-18 | 2009-07-08 | 姚成义 | Cuttage and breeding method of Aquilaria sinensis |
| CN104170734A (en) * | 2014-09-04 | 2014-12-03 | 南京标科生物科技有限公司 | Rapid propagation method of burmann cinnamon bark |
| CN104170647A (en) * | 2014-09-12 | 2014-12-03 | 南京通泽农业科技有限公司 | Rapid propagation method for cinnamomum burmannii through cuttage |
| CN104872181A (en) * | 2015-05-12 | 2015-09-02 | 柳州市耕青科技有限公司 | Special rooting agent for camphor trees |
-
2015
- 2015-11-10 CN CN201510765630.2A patent/CN105230321B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263765A (en) * | 2008-05-13 | 2008-09-17 | 湖南省新晃县龙脑开发有限责任公司 | Cirmamonun camphora clone scale fast propagation seedling-breeding method |
| CN101473736A (en) * | 2008-05-18 | 2009-07-08 | 姚成义 | Cuttage and breeding method of Aquilaria sinensis |
| CN104170734A (en) * | 2014-09-04 | 2014-12-03 | 南京标科生物科技有限公司 | Rapid propagation method of burmann cinnamon bark |
| CN104170647A (en) * | 2014-09-12 | 2014-12-03 | 南京通泽农业科技有限公司 | Rapid propagation method for cinnamomum burmannii through cuttage |
| CN104872181A (en) * | 2015-05-12 | 2015-09-02 | 柳州市耕青科技有限公司 | Special rooting agent for camphor trees |
Non-Patent Citations (2)
| Title |
|---|
| 天然右旋龙脑新资源―梅片树;卓万廉;《中国野生植物资源》;19891231(第04期);第20页 * |
| 天然右旋龙脑新资源――梅片树的研究;李毓敬等;《Journal of Integrative Plant Biology》;19870531;第29卷(第05期);第527-531页 * |
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Address after: 514641 Guangdong Huaqingyuan Biotechnology Co., Ltd., Pingyuan Industrial Park, huanghuapi, Dazhe Town, Pingyuan County, Meizhou City, Guangdong Province Patentee after: GUANGDONG HUAQINGYUAN BIOTECHNOLOGY Co.,Ltd. Patentee after: Forestry Research Institute of Meizhou Academy of agricultural and Forestry Sciences Address before: 514641 Guangdong Huaqingyuan Biotechnology Co., Ltd., Pingyuan Industrial Park, huanghuapi, Dazhe Town, Pingyuan County, Meizhou City, Guangdong Province Patentee before: GUANGDONG HUAQINGYUAN BIOTECHNOLOGY Co.,Ltd. Patentee before: Meizhou Forestry Science Research Institute |
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Application publication date: 20160113 Assignee: Meizhou jiayimei chip tree planting professional cooperative Assignor: GUANGDONG HUAQINGYUAN BIOTECHNOLOGY Co.,Ltd.|Forestry Research Institute of Meizhou Academy of agricultural and Forestry Sciences Contract record no.: X2022440000054 Denomination of invention: A cutting breeding method of Dipterocarpus vulgaris Granted publication date: 20180413 License type: Exclusive License Record date: 20220721 |
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Address after: 514641 Guangdong Huaqingyuan Biotechnology Co., Ltd., Pingyuan Industrial Park, huanghuapi, Dazhe Town, Pingyuan County, Meizhou City, Guangdong Province Patentee after: Guangdong Huaqingyuan Biotechnology Co.,Ltd. Country or region after: China Patentee after: Forestry Research Institute of Meizhou Academy of agricultural and Forestry Sciences Address before: 514641 Guangdong Huaqingyuan Biotechnology Co., Ltd., Pingyuan Industrial Park, huanghuapi, Dazhe Town, Pingyuan County, Meizhou City, Guangdong Province Patentee before: GUANGDONG HUAQINGYUAN BIOTECHNOLOGY Co.,Ltd. Country or region before: China Patentee before: Forestry Research Institute of Meizhou Academy of agricultural and Forestry Sciences |