CN105230321A - Cuttage breeding method for borneol type cinnamomum burmannii tree - Google Patents
Cuttage breeding method for borneol type cinnamomum burmannii tree Download PDFInfo
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- CN105230321A CN105230321A CN201510765630.2A CN201510765630A CN105230321A CN 105230321 A CN105230321 A CN 105230321A CN 201510765630 A CN201510765630 A CN 201510765630A CN 105230321 A CN105230321 A CN 105230321A
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- cutting
- cuttage
- breeding method
- borneol
- cuttage breeding
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- DTGKSKDOIYIVQL-WEDXCCLWSA-N (+)-borneol Chemical compound C1C[C@@]2(C)[C@@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-WEDXCCLWSA-N 0.000 title claims abstract description 54
- REPVLJRCJUVQFA-UHFFFAOYSA-N (-)-isopinocampheol Natural products C1C(O)C(C)C2C(C)(C)C1C2 REPVLJRCJUVQFA-UHFFFAOYSA-N 0.000 title claims abstract description 49
- CKDOCTFBFTVPSN-UHFFFAOYSA-N borneol Natural products C1CC2(C)C(C)CC1C2(C)C CKDOCTFBFTVPSN-UHFFFAOYSA-N 0.000 title claims abstract description 49
- 229940116229 borneol Drugs 0.000 title claims abstract description 49
- DTGKSKDOIYIVQL-UHFFFAOYSA-N dl-isoborneol Natural products C1CC2(C)C(O)CC1C2(C)C DTGKSKDOIYIVQL-UHFFFAOYSA-N 0.000 title claims abstract description 49
- 238000009395 breeding Methods 0.000 title claims abstract description 35
- 244000037185 Cinnamomum burmannii Species 0.000 title abstract 4
- 235000014487 Cinnamomum burmannii Nutrition 0.000 title abstract 4
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000005520 cutting process Methods 0.000 claims description 107
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 46
- 239000000243 solution Substances 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 23
- 241001597008 Nomeidae Species 0.000 claims description 21
- 239000011449 brick Substances 0.000 claims description 18
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 16
- 239000004563 wettable powder Substances 0.000 claims description 14
- 239000008187 granular material Substances 0.000 claims description 13
- 239000007921 spray Substances 0.000 claims description 12
- 238000005507 spraying Methods 0.000 claims description 12
- 230000003020 moisturizing effect Effects 0.000 claims description 11
- 239000000645 desinfectant Substances 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 9
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 8
- 229940033663 thimerosal Drugs 0.000 claims description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 7
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 7
- 241001330002 Bambuseae Species 0.000 claims description 7
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 7
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 7
- 239000011425 bamboo Substances 0.000 claims description 7
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 239000002985 plastic film Substances 0.000 claims description 7
- 229920006255 plastic film Polymers 0.000 claims description 7
- 230000000384 rearing effect Effects 0.000 claims description 7
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 7
- 239000013522 chelant Substances 0.000 claims description 6
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 6
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 claims description 5
- 229910052709 silver Inorganic materials 0.000 claims description 4
- 239000004332 silver Substances 0.000 claims description 4
- -1 silver ions Chemical class 0.000 claims description 4
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 239000003899 bactericide agent Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 230000000855 fungicidal effect Effects 0.000 claims description 3
- 239000000417 fungicide Substances 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 9
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 230000004071 biological effect Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 101710134784 Agnoprotein Proteins 0.000 description 11
- 230000006698 induction Effects 0.000 description 10
- 239000005648 plant growth regulator Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- DTGKSKDOIYIVQL-NQMVMOMDSA-N (+)-Borneol Natural products C1C[C@]2(C)[C@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-NQMVMOMDSA-N 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 230000000975 bioactive effect Effects 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- ZSILVJLXKHGNPL-UHFFFAOYSA-L S(=S)(=O)([O-])[O-].[Ag+2] Chemical compound S(=S)(=O)([O-])[O-].[Ag+2] ZSILVJLXKHGNPL-UHFFFAOYSA-L 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000723347 Cinnamomum Species 0.000 description 2
- 240000005636 Dryobalanops aromatica Species 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 241000218195 Lauraceae Species 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000000873 masking effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000007182 Ochroma pyramidale Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 229960000846 camphor Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a cuttage breeding method for a borneol type cinnamomum burmannii tree, and belongs to the technical field of tree cuttage breeding methods. The method is technically characterized by comprising the steps of 1, cuttage collection; 2, cuttage treatment, wherein collected semi-lignified newly-born branches are trimmed into cuttages with the length being 5-8 cm, each base portion is sheared into an inclined plane, every 50-60 cuttages are bundled up into one bundle, the lower ends of the cuttages are soaked with a biological activity inductive agent solution for 8-12 h for use; 3, slotting machine preparation; 4 cuttage. The cuttage breeding method for the borneol type cinnamomum burmannii tree is high in survival rate and short in cuttage period and is used for cuttage breeding of the borneol type cinnamomum burmannii tree.
Description
Technical field
The present invention relates to a kind of trees mating system, more particularly, particularly relate to the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type.
Background technology
The cloudy fragrant tree of borneol type and Borneolum tree (Dryobalanopsaromatica) belong to Lauraceae (Lauraceae) Cinnamomum (Cinnamomum) aiphyllium, external form and the fragrant plant (CinnamomumburmanniiB1.) of the moon are as good as, and are physiology or the cloudy perfume of chemical type being rich in d-Bomeol.Borneolum contained in its branches and leaves, formal name used at school " natural d-borneol ", it is a kind of fine perfumery, also be a kind of famous and precious rare medicinal material, be widely used in the industries such as medicine, cosmetics, spices, because it can suppress or the generation of the multiple degenerative disorders such as epidemic prevention system degradation and angiocardiopathy effectively, applying very extensive clinically, is the main component of multiple famous-brand and high-quality Chinese patent drug.The borneo camphor tree tree root originating in Indonesia Sumatra archipelago is the early stage main source of natural d-borneol, over nearly more than 100 years, the natural d-borneol of China mainly relies on import to satisfy the demand, due to locality over-exploitation for a long time, natural resources destroys serious, and local government has forbidden cutting down trees getting brain in addition, thus, natural d-borneol product is in great shortage, more and more concerned.
In the fragrant tree branches and leaves of common the moon, effectively product assay is low for d-Bomeol etc., and in the cloudy fragrant branches and leaves of borneol type of seed selection, effectively product assay is high, utilize cutting propagation mode (vegetative propagation) can keep merit, and quickly breeding goes out breeding nursery stock.Setting up raw material forestbase is in the above described manner solve important channel in short supply, medicine source.Therefore, the cuttage breeding method researching and developing the cloudy fragrant tree of borneol type that a kind of survival rate is high, the cuttage cycle is short is necessary.
Summary of the invention
The object of the invention is to for above-mentioned the deficiencies in the prior art, the cuttage breeding method of the cloudy fragrant tree of borneol type that a kind of survival rate is high, the cuttage cycle is short is provided.
Technical scheme of the present invention is achieved in that the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type, and the method comprises the steps:
(1) gather cutting: annual October to December, during the newborn branch semi-lignified of the cloudy fragrant tree of borneol type, newborn for semi-lignified branch is adopted down, for subsequent use;
(2) cutting process: newborn for the semi-lignified of collection branch is trimmed to the cutting that length is 5 ~ 8cm, base portion is cut into inclined-plane, with every 50 ~ 60 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 8 ~ 12 hours, for subsequent use;
(3) slotting machine prepares: cutting bed is located in cool canopy, surrounding common brick brick work, and fill the granule shape yellow mud on top layer, non-ground in slotting machine as cutting medium, thickness 10 ~ 15cm, flattens and water permeable rear bed surface thimerosal spray disinfectant, for subsequent use;
(4) cuttage: the small bamboo spike consistent with cutting by thickness delineates shallow ridges on slotting machine, after be that 3 ~ 5cm inserts in ditch by the cutting handled well by spacing in the rows, insert the dark 3 ~ 5cm of soil, delineate cuttage line by line by line-spacing 5cm, water of drenching after being plugged, and spraying fungicide once, lid diaphragm seal moisturizing, film humidity controls in 70% ~ 90% scope, and the additional lid layer obscurity of film is the sunshade net of 70 ~ 75%, checks once every 10 ~ 15 days; Select temperature automatically controlled moisture control device automatically to control control and management to shady temperature of shed, within 110 days ~ 130 days, throw off film, container seedling cultivated by upper bag of transplanting seedlings after 140 days ~ 160 days.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, the described cutting process of step (2) is specially: newborn for the semi-lignified of collection branch is trimmed to the cutting that length is 5 ~ 8cm, retain cutting top vane 1 ~ 2, base portion is cut into 40 ° ~ 60 ° inclined-planes, with every 50 ~ 60 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 8 ~ 12 hours, for subsequent use.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, biologically active derivant solution described in step (2) is methyl α-naphthyl acetate, draws the mixed solution of diindyl butyric acid and silver nitrate, its preparation method is: be first 1 ~ 3 ﹕ 1 compound concentration by methyl α-naphthyl acetate with the mass ratio drawing diindyl butyric acid be the mixed solution of 100 ~ 200mg/L, then silver nitrate is added according to the total amount of mixed solution, the addition of silver nitrate is that often liter of mixed solution adds 1 ~ 10mg, obtains biologically active derivant solution.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, described silver nitrate can adopt the silver ion chelate containing equivalent concentration of silver ions to replace.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, the described slotting machine of step (3) prepares to be specially: cutting bed is located in cool canopy, surrounding common brick brick work, the granule shape yellow mud on top layer, non-ground is filled as cutting medium in slotting machine, thickness 10 ~ 15cm, flattens and waters permeable rear bed surface thimerosal spray disinfectant, then leaves standstill 5 ~ 10 days with covered rearing with plastic film, within 1 ~ 2 day before use, throw off film, water permeable for subsequent use.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, described in step (3), the length of cutting bed is 3 ~ 5m, and width is 1 ~ 1.5m, and the degree of depth is 20 ~ 40cm.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, described in step (3), the diameter of granule shape yellow mud is 2 ~ 5mm.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, thimerosal described in step (3) is the 50% carbendazol wettable powder solution of 1000 times.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, bactericide described in step (4) is the 70% thiophanate methyl wettable powder agent solution of 2500 times.
In the cuttage breeding method of the cloudy fragrant tree of above-mentioned a kind of borneol type, in cool canopy described in step (4), temperature requirement controls lower than 30 DEG C.
After the present invention adopts said method, compared with prior art, there is following advantage:
(1) cutting plantation and survival rate are widely different because plant variety is different, particularly different plants should select the plant growth regulator (plant hormone) of variety classes and concentration or its combination to carry out cutting process, and the present invention adopts to have and promotes bioactive chemical induction (Ag
+) with two plant growth regulators methyl α-naphthyl acetates (NAA) with draw the biologically active derivant solution immersion treatment cutting that diindyl butyric acid (IBA) mixes, obviously can promote the cloudy fragrant tree cutting base portion calli induction of borneol type, be conducive to differentiating healthy and strong root system, effectively improve rooting of cuttings rate and survival rate thereof; Experiment proves, adopts the silver ion (Ag of silver nitrate or equivalent concentration
+) mix formed biologically active derivant solution with two plant growth regulators, there is same effect.
(2) traditional cutting medium selects yellow mud, river sand or perlite etc. to use after certain proportion mixing, cutting medium of the present invention selects the granule shape yellow mud on top layer, non-ground, few containing the foreign material such as miscellaneous bacteria and plant seed, the object reducing weeds and damage by disease and insect generation can be reached, be conducive to improving cuttage survival rate; Select the granule shape yellow mud of 100%1 ~ 5mm diameter to make cutting medium, have good moisture capacity, make the contiguity performance of root system and soil good, and cutting angular cut and cutting medium contact surface are comparatively large, cuttage more easily survives;
(3) cuttage complete after conventional management mode be the temperature and humidity being regulated slotting machine by artificial or automatic control equipment, but repeated multiple times water spray easily causes soil compaction to affect cuttage quality, it is remarkable that the present invention adopts masking film to seal training method moistening effect completely after cuttage completes, can obviously reduce water spray number of times, cutting is simultaneously affected by the external environment little, the chance of obvious minimizing secondary microbiological contamination, the management workloads such as spray medicine of spraying water after cuttage obviously reduce, and survival rate improves greatly;
(4) lower according to the cloudy fragrant tree early development light saturation point of borneol type characteristic, after cutting bed masking film, additional lid layer obscurity is the sunshade net of 70 ~ 75%, strong sunlight can be avoided directly to shine injury to cutting blade and rudiment, improve cuttage survival rate.Therefore, cottage method of the present invention successfully can breed the cloudy fragrant sapling of borneol type, and cuttage survival rate can reach more than 90%, can reach 96.3%, significantly reduces seedling cost and meets the demand in market.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, but do not form any limitation of the invention.
The cuttage breeding method of the cloudy fragrant tree of a kind of borneol type of the present invention, the method comprises the steps:
(1) gather cutting: annual October to December, during the newborn branch semi-lignified of the cloudy fragrant tree of borneol type, newborn for semi-lignified branch is adopted down, for subsequent use;
(2) cutting process: newborn for the semi-lignified of collection branch is trimmed to the cutting that length is 5 ~ 8cm, retain cutting top vane 1 ~ 2, base portion is cut into 40 ° ~ 60 ° inclined-planes, with every 50 ~ 60 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 8 ~ 12 hours, for subsequent use; Wherein, described biologically active derivant solution is methyl α-naphthyl acetate, draws the mixed solution of diindyl butyric acid and silver nitrate, its preparation method is: be first 1 ~ 3 ﹕ 1 compound concentration by methyl α-naphthyl acetate (NAA) with the mass ratio drawing diindyl butyric acid (IBA) be the mixed solution of 100 ~ 200mg/L, then silver nitrate is added according to the total amount of mixed solution, the addition of silver nitrate is that often liter of mixed solution adds 1 ~ 10mg, obtains biologically active derivant solution.Described silver nitrate (AgNO
3) silver ion (Ag of equivalent molar concentration (μm ol/L) can be adopted
+) chelate.
(3) slotting machine prepares: length is 3 ~ 5m, width is 1 ~ 1.5m, the degree of depth is that the cutting bed of 20 ~ 40cm is located in cool canopy, surrounding common brick brick work, fill top layer, non-ground in slotting machine and diameter be the granule shape yellow mud of 2 ~ 5mm as cutting medium, thickness 10 ~ 15cm, flatten and water permeable rear bed surface thimerosal spray disinfectant, then leave standstill 5 ~ 10 days with covered rearing with plastic film, within 1 ~ 2 day before use, throw off film, water permeable for subsequent use; Described thimerosal is the 50% carbendazol wettable powder solution of 1000 times.
(4) cuttage: the small bamboo spike consistent with cutting by thickness delineates shallow ridges on slotting machine, after be that 3 ~ 5cm inserts in ditch by the cutting handled well by spacing in the rows, insert the dark 3 ~ 5cm of soil, delineate cuttage line by line by line-spacing 5cm, water of drenching after being plugged, and spraying fungicide once, lid diaphragm seal moisturizing, film humidity controls in 70% ~ 90% scope, and the additional lid layer obscurity of film is the sunshade net of 70 ~ 75%, checks once every 10 ~ 15 days; Select temperature automatically controlled moisture control device automatically to control control and management to shady temperature of shed, in cool canopy, temperature requirement controls lower than 30 DEG C; Within 110 days ~ 130 days, throw off film, container seedling cultivated by upper bag of transplanting seedlings after 140 days ~ 160 days.Described bactericide is the 70% thiophanate methyl wettable powder agent solution of 2500 times.
Embodiment 1
A cuttage breeding method for the cloudy fragrant tree of borneol type, the method comprises the steps:
(1), in annual early October, during the newborn branch semi-lignified of the cloudy fragrant tree of borneol type, newborn for semi-lignified branch is adopted down, for subsequent use;
(2) cutting process: the semi-lignified branch of collection is trimmed to the cutting that length is 5 ~ 8cm, retain cutting top vane 1 ~ 2, base portion is cut into 40 ° ~ 60 ° inclined-planes, with every 50 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 12 hours respectively, the biologically active derivant solution type that experimental group adopts and concentration as follows: 1. group: silver nitrate (AgNO
3) 1mg/L+NAA50mg/L+IBA50mg/L; 2. group: silver nitrate (AgNO
3) 1mg/L+NAA100mg/L+IBA50mg/L; 3. group: silver nitrate (AgNO
3) 1mg/L+NAA150mg/L+IBA50mg/L; 4. group: NAA50mg/L+IBA50mg/L.
(3) slotting machine prepares: length is 3m, width is 1m, the degree of depth is that the cutting bed of 25cm is located in cool canopy, surrounding common brick brick work, fill top layer, non-ground in slotting machine and diameter be the granule shape yellow mud of 2 ~ 5mm as cutting medium, filling thickness 10cm, flatten and water permeable rear bed surface 50% carbendazol wettable powder 1000 times of liquid spray disinfectants once, then with covered rearing with plastic film process 8 days, within 1 day before use, throw off film, water permeable for subsequent use;
(4) cuttage: the small bamboo spike consistent with cutting by thickness delineates shallow ridges on slotting machine, after be that 3 ~ 5cm inserts in ditch by the cutting handled well by spacing in the rows, insert the dark 3 ~ 5cm of soil, delineate cuttage line by line by line-spacing 5cm, water of drenching after being plugged, and the 70% thiophanate methyl wettable powder agent solution spraying 2500 times once, lid diaphragm seal moisturizing, the additional lid layer obscurity of film is 75% sunshade net, checks once, moisturizing of spraying water if desired every 10 days.Shady temperature of shed controls lower than 30 DEG C, within 120 days, throws off film, and container seedling cultivated by upper bag of transplanting seedlings after 150 days.
Result shows: cutting process adopts to have and promotes bioactive chemical induction (Ag
+) with the mixed solution of two plant growth regulators NAA+IBA (1 ~ 3 ﹕ 1), obviously can promote the cloudy fragrant tree cutting base portion calli induction of borneol type, be conducive to differentiating healthy and strong root system, each experimental group rooting of cuttings rate reaches 1. group: 88.5%; 2. group: 93.3%; 3. group: 90.8%; 4. group: 80.3%.
Embodiment 2
A cuttage breeding method for the cloudy fragrant tree of borneol type, the method comprises the steps:
(1), in annual late October, during the newborn branch semi-lignified of the cloudy fragrant tree of borneol type, newborn for semi-lignified branch is adopted down, for subsequent use;
(2) cutting process: the semi-lignified branch of collection is trimmed to the cutting that length is 5 ~ 8cm, retain cutting top vane 1 ~ 2, base portion is cut into 40 ° ~ 60 ° inclined-planes, with every 50 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 8 hours respectively, the biologically active derivant solution type that experimental group adopts and concentration as follows: 1. group: silver thiosulfate Ag
2s
2o
3(silver ion Ag
+chelate 3 μm of ol/L)+NAA50mg/L+IBA50mg/L; 2. group: silver thiosulfate Ag
2s
2o
3(Ag
+chelate) 3 μm of ol/L+NAA100mg/L+IBA50mg/L; 3. group: silver thiosulfate Ag
2s
2o
3(Ag
+chelate) 3 μm of ol/L+NAA150mg/L+IBA50mg/L; 4. group: NAA50mg/L+IBA50mg/L.Silver thiosulfate 3 μm of ol/L silver ions and silver nitrate (AgNO
3) 2mg/L is suitable.
(3) slotting machine prepares: length is 4m, width is 1.5m, the degree of depth is that the cutting bed of 40cm is located in cool canopy, surrounding common brick brick work, fill top layer, non-ground in slotting machine and diameter be the granule shape yellow mud of 2 ~ 5mm as cutting medium, filling thickness 15cm, flatten and water permeable rear bed surface 50% carbendazol wettable powder 1000 times of liquid spray disinfectants once, then with covered rearing with plastic film process 6 days, within 2 days before use, throw off film, water permeable for subsequent use;
(4) cuttage: the small bamboo spike consistent with cutting by thickness delineates shallow ridges on slotting machine, after be that 3 ~ 5cm inserts in ditch by the cutting handled well by spacing in the rows, insert the dark 3 ~ 5cm of soil, delineate cuttage line by line by line-spacing 5cm, water of drenching after being plugged, and the 70% thiophanate methyl wettable powder agent solution spraying 2500 times once, lid diaphragm seal moisturizing, the additional lid layer obscurity of film is 75% sunshade net, checks once, moisturizing of spraying water if desired every 15 days.Shady temperature of shed controls lower than 30 DEG C, within 110 days, throws off film, and container seedling cultivated by upper bag of transplanting seedlings after 140 days.
Result shows: cutting process adopts to have and promotes bioactive chemical induction (Ag
+) with the mixed solution of two plant growth regulators NAA+IBA (1 ~ 3 ﹕ 1), obviously can promote the cloudy fragrant tree cutting base portion calli induction of borneol type, be conducive to differentiating healthy and strong root system, each experimental group rooting of cuttings rate reaches 1. group: 91.5%; 2. group: 96.3%; 3. group: 92.8%; 4. group: 82.9%.
Embodiment 3
A cuttage breeding method for the cloudy fragrant tree of borneol type, the method comprises the steps:
(1), annual mid-November, during the newborn branch semi-lignified of the cloudy fragrant tree of borneol type, newborn for semi-lignified branch is adopted down, for subsequent use;
(2) cutting process: the semi-lignified branch of collection is trimmed to the cutting that length is 5 ~ 8cm, retain cutting top vane 1 ~ 2, base portion is cut into 40 ° ~ 60 ° inclined-planes, with every 60 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 10 hours respectively, the biologically active derivant solution type that experimental group adopts and concentration as follows: 1. group: silver nitrate (AgNO
3) 5mg/L+NAA50mg/L+IBA50mg/L; 2. group: silver nitrate (AgNO
3) 5mg/L+NAA100mg/L+IBA50mg/L; 3. group: silver nitrate (AgNO
3) 5mg/L+NAA150mg/L+IBA50mg/L; 4. group: NAA50mg/L+IBA50mg/L.
(3) slotting machine prepares: length is 5m, width is 1.5m, the degree of depth is that the cutting bed of 20cm is located in cool canopy, surrounding common brick brick work, fill top layer, non-ground in slotting machine and diameter be the granule shape yellow mud of 2 ~ 5mm as cutting medium, filling thickness 12cm, flatten and water permeable rear bed surface 50% carbendazol wettable powder 1000 times of liquid spray disinfectants once, then with covered rearing with plastic film process 10 days, within 1 day before use, throw off film, water permeable for subsequent use;
(4) cuttage: the small bamboo spike consistent with cutting by thickness delineates shallow ridges on slotting machine, after be that 3 ~ 5cm inserts in ditch by the cutting handled well by spacing in the rows, insert the dark 3 ~ 5cm of soil, delineate cuttage line by line by line-spacing 5cm, water of drenching after being plugged, and the 70% thiophanate methyl wettable powder agent solution spraying 2500 times once, lid diaphragm seal moisturizing, the additional lid layer obscurity of film is 75% sunshade net, checks once, moisturizing of spraying water if desired every 10 days.Shady temperature of shed should control lower than 30 DEG C, within 130 days, throws off film, and container seedling cultivated by upper bag of transplanting seedlings after 160 days.
Result shows: cutting process adopts to have and promotes bioactive chemical induction (Ag
+) with the mixed solution of two plant growth regulators NAA+IBA (1 ~ 3 ﹕ 1), obviously can promote the cloudy fragrant tree cutting base portion calli induction of borneol type, be conducive to differentiating healthy and strong root system, each experimental group rooting of cuttings rate reaches 1. group: 92.3%; 2. group: 95.8%; 3. group: 91.6%; 4. group: 83.0%.
Embodiment 4
A cuttage breeding method for the cloudy fragrant tree of borneol type, the method comprises the steps:
(1), in annual early December, during the newborn branch semi-lignified of the cloudy fragrant tree of borneol type, newborn for semi-lignified branch is adopted down, for subsequent use;
(2) cutting process: the semi-lignified branch of collection is trimmed to the cutting that length is 5 ~ 8cm, retain cutting top vane 1 ~ 2, base portion is cut into 40 ° ~ 60 ° inclined-planes, with every 55 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 9 hours respectively, the biologically active derivant solution type that experimental group adopts and concentration as follows: 1. group: silver nitrate (AgNO
3) 10mg/L+NAA50mg/L+IBA50mg/L; 2. group: silver nitrate (AgNO
3) 10mg/L+NAA100mg/L+IBA50mg/L; 3. group: silver nitrate (AgNO
3) 10mg/L+NAA150mg/L+IBA50mg/L; 4. group: NAA50mg/L+IBA50mg/L.
(3) slotting machine prepares: length is 3.5m, width is 1m, the degree of depth is that the cutting bed of 30cm is located in cool canopy, surrounding common brick brick work, fill top layer, non-ground in slotting machine and diameter be the granule shape yellow mud of 2 ~ 5mm as cutting medium, filling thickness 14cm, flatten and water permeable rear bed surface 50% carbendazol wettable powder 1000 times of liquid spray disinfectants once, then with covered rearing with plastic film process 5 days, within 2 days before use, throw off film, water permeable for subsequent use;
(4) cuttage: the small bamboo spike consistent with cutting by thickness delineates shallow ridges on slotting machine, after be that 3 ~ 5cm inserts in ditch by the cutting handled well by spacing in the rows, insert the dark 3 ~ 5cm of soil, delineate cuttage line by line by line-spacing 5cm, water of drenching after being plugged, and the 70% thiophanate methyl wettable powder agent solution spraying 2500 times once, lid diaphragm seal moisturizing, the additional lid layer obscurity of film is 75% sunshade net, checks once, moisturizing of spraying water if desired every 15 days.Shady temperature of shed should control lower than 30 DEG C, within 120 days, throws off film, and container seedling cultivated by upper bag of transplanting seedlings after 150 days.
Result shows: cutting process adopts to have and promotes bioactive chemical induction (Ag
+) with the mixed solution of two plant growth regulators NAA+IBA (1 ~ 3 ﹕ 1), obviously can promote the cloudy fragrant tree cutting base portion calli induction of borneol type, be conducive to differentiating healthy and strong root system, each experimental group rooting of cuttings rate reaches 1. group: 90.1%; 2. group: 93.8%; 3. group: 90.6%; 4. group: 81.1%.
Above illustrated embodiment is better embodiment of the present invention, only be used for conveniently the present invention being described, not any pro forma restriction is done to the present invention, have in any art and usually know the knowledgeable, if do not depart from the present invention carry in the scope of technical characteristic, utilize the Equivalent embodiments that the done local of disclosed technology contents is changed or modified, and do not depart from technical characteristic content of the present invention, all still belong in the scope of the technology of the present invention feature.
Claims (10)
1. a cuttage breeding method for the cloudy fragrant tree of borneol type, it is characterized in that, the method comprises the steps:
(1) gather cutting: annual October to December, during the newborn branch semi-lignified of the cloudy fragrant tree of borneol type, newborn for semi-lignified branch is adopted down, for subsequent use;
(2) cutting process: newborn for the semi-lignified of collection branch is trimmed to the cutting that length is 5 ~ 8cm, base portion is cut into inclined-plane, with every 50 ~ 60 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 8 ~ 12 hours, for subsequent use;
(3) slotting machine prepares: cutting bed is located in cool canopy, surrounding common brick brick work, and fill the granule shape yellow mud on top layer, non-ground in slotting machine as cutting medium, thickness 10 ~ 15cm, flattens and water permeable rear bed surface thimerosal spray disinfectant, for subsequent use;
(4) cuttage: the small bamboo spike consistent with cutting by thickness delineates shallow ridges on slotting machine, after be that 3 ~ 5cm inserts in ditch by the cutting handled well by spacing in the rows, insert the dark 3 ~ 5cm of soil, delineate cuttage line by line by line-spacing 5cm, water of drenching after being plugged, and spraying fungicide once, lid diaphragm seal moisturizing, film humidity controls in 70% ~ 90% scope, and the additional lid layer obscurity of film is the sunshade net of 70 ~ 75%, checks once every 10 ~ 15 days; Select temperature automatically controlled moisture control device automatically to control control and management to shady temperature of shed, within 110 days ~ 130 days, throw off film, container seedling cultivated by upper bag of transplanting seedlings after 140 days ~ 160 days.
2. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 1, it is characterized in that, the described cutting process of step (2) is specially: newborn for the semi-lignified of collection branch is trimmed to the cutting that length is 5 ~ 8cm, retain cutting top vane 1 ~ 2, base portion is cut into 40 ° ~ 60 ° inclined-planes, with every 50 ~ 60 root cuttings for a bundle ties, the lower end of cutting to be soaked in biologically active derivant solution 8 ~ 12 hours, for subsequent use.
3. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 1 and 2, it is characterized in that, biologically active derivant solution described in step (2) is methyl α-naphthyl acetate, draws the mixed solution of diindyl butyric acid and silver nitrate, its preparation method is: be first 1 ~ 3 ﹕ 1 compound concentration by methyl α-naphthyl acetate with the mass ratio drawing diindyl butyric acid be the mixed solution of 100 ~ 200mg/L, then silver nitrate is added according to the total amount of mixed solution, the addition of silver nitrate is that often liter of mixed solution adds 1 ~ 10mg, obtains biologically active derivant solution.
4. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 3, it is characterized in that, described silver nitrate can adopt the silver ion chelate of equivalent concentration of silver ions to replace.
5. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 1, it is characterized in that, the described slotting machine of step (3) prepares to be specially: cutting bed is located in cool canopy, surrounding common brick brick work, fills the granule shape yellow mud on top layer, non-ground as cutting medium, thickness 10 ~ 15cm in slotting machine, flatten and water permeable rear bed surface thimerosal spray disinfectant, then leave standstill 5 ~ 10 days with covered rearing with plastic film, within 1 ~ 2 day before use, throw off film, water permeable for subsequent use.
6. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 1 or 5, it is characterized in that, described in step (3), the length of cutting bed is 3 ~ 5m, and width is 1 ~ 1.5m, and the degree of depth is 20 ~ 40cm.
7. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 1 or 5, it is characterized in that, described in step (3), the diameter of granule shape yellow mud is 1 ~ 5mm.
8. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 1 or 5, it is characterized in that, thimerosal described in step (3) is the 50% carbendazol wettable powder solution of 1000 times.
9. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 1, it is characterized in that, bactericide described in step (4) is the 70% thiophanate methyl wettable powder agent solution of 2500 times.
10. the cuttage breeding method of the cloudy fragrant tree of a kind of borneol type according to claim 1, is characterized in that, in cool canopy described in step (4), temperature requirement controls lower than 30 DEG C.
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