CN105213993A - One improves cachectic Chinese medicine composition of intestinal cancer and preparation method thereof - Google Patents
One improves cachectic Chinese medicine composition of intestinal cancer and preparation method thereof Download PDFInfo
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Abstract
The invention discloses one and improve cachectic Chinese medicine composition of intestinal cancer and preparation method thereof, this Chinese medicine composition comprises the crude drug of following weight portion: Radix Astragali 20-35 part, Radix Codonopsis 20-30 part, Rhizoma Curcumae 30-40 part, Semen Lablab Album 15-25 part, raw pearl barley 15-20 part, Flos Lonicerae 10-20 part, Rhizoma Atractylodis Macrocephalae (parched) 5-10 part, Pericarpium Citri Reticulatae 5-10 part.The present invention prepares simply, by the collaborative onset of each crude drug play good integrally-regulated, improve cachexia amyotrophy, suppress muscle protein to decompose, to alleviate and opposing cachexia body weight Progressive symmetric erythrokeratodermia declines, improves life in patients, extends the effects such as survival of patients phase, intervening intestinal cancer cachexia for prevention has good effect.
Description
Technical field
The present invention relates to a kind of medicine improving carcinemia patients ' life quality, be specifically related to one and improve cachectic Chinese medicine composition of intestinal cancer and preparation method thereof, belong to medical art.
Background technology
Cachexia (cachexia) is a medical terminology, derives from Hellenic " kakos " and " hexis ", literally means " severe situation ".Clinically cachexia be defined as a kind of with anorexia, anemia, lose weight as the syndrome of cardinal symptom, it can betide various diseases, comprise tumor, AIDS, severe trauma, Post operation, malabsorption and serious septicemia etc., the cachexia wherein occurred together with tumor is the most common, is called tumor cachexia.
Late period more than 80% there is carcinemia in intestinal cancer patient, has a strong impact on patients ' life quality, shortens life cycle, reduces the sensitivity of chemotherapy or treatment, and increasing complication and occur, is the major reason of cancer death.Unsatisfactory to the treatment of intestinal cancer cachexia at present, conventional therapy such as appetite stimulator etc. can be put on weight, but not obvious to the minimizing curative effect of the lean tissue mass groups such as muscle.The treatment of simple cell factor antibody can destroy body's immunity, accelerates tumor growth.
The traditional Chinese medical science thinks that the cachectic pathogenesis of intestinal cancer is that organ yin-yang QI and blood is deficient and has ecchymosis concurrently, and the symptoms such as the poor appetite that patient shows, emaciated physique are all due to obstinate disease, function obstacle, and negative and positive of qi and blood is deficient, body lose in moisten support caused by.Therefore, QI invigorating warming YANG, activating blood and eliminatng dampness, detoxicating and resolving stagnation of pathogens, it is the basic therapy of Chinese traditional treatment carcinemia, the cachectic research for the treatment of intestinal cancer is carried out under this thought guidance, and in conjunction with previous research, initiative treatment intestinal cancer cachexia Chinese medicine formula, decline to alleviating and improve intestinal cancer cachectic patients body weight Progressive symmetric erythrokeratodermia, amyotrophy, improves life in patients, extends the survival of patients phase.
In western medicine intestinal cancer process, the phenomenon of Clinical practice chemotherapy is quite general, drug resistance is caused to increase year by year, and the side effect of chemotherapy is also very serious, not only be unprofitable to the improvement of the state of an illness, also likely increase the weight of the state of an illness, how to improve the life life quality of intestinal cancer cachectic patients, become China and even a challenge facing of the whole world.Motherland's medical science is advocated to play patient's autoimmunity with the method be in harmonious proportion, the organic conception emphasized and prophylactic treatment, occupy more important status in intestinal cancer treatment of cachexia, makes patient experiences stable disease by appropriateness intervention, reach " people's tumor coexists ", the object of long-term band tumor existence.
Summary of the invention
The invention provides one and improve the cachectic Chinese medicine composition of intestinal cancer, said composition have integrally-regulated, alleviate and improve intestinal cancer cachectic patients body weight Progressive symmetric erythrokeratodermia and decline, improve cachexia amyotrophy, improve life in patients, extend the effect such as survival of patients phase.
Present invention also offers the preparation method of this Chinese medicine composition, its preparation is simple, evident in efficacy, is convenient to suitability for industrialized production and clinical practice.
The present invention is achieved by the following technical solutions:
One improves the cachectic Chinese medicine composition of intestinal cancer, and this Chinese medicine composition comprises the crude drug of following weight portion: Radix Astragali 20-35 part, Radix Codonopsis 20-30 part, Rhizoma Curcumae 30-40 part, Semen Lablab Album 15-25 part, raw pearl barley 15-20 part, Flos Lonicerae 10-20 part, Rhizoma Atractylodis Macrocephalae (parched) 5-10 part, Pericarpium Citri Reticulatae 5-10 part.
Further, when above-mentioned Chinese medicine composition selects the crude drug of following preferred weight part, effect is better: the Radix Astragali 25 parts, Radix Codonopsis 22 parts, Rhizoma Curcumae 35 parts, Semen Lablab Album 20 parts, raw pearl barley 18 parts, Flos Lonicerae 15 parts, Rhizoma Atractylodis Macrocephalae (parched) 6 parts, Pericarpium Citri Reticulatae 6 parts.
Further, in order to reach better effect, Herba Epimedii, Retinervus Luffae Fructus, Ramulus Euonymi three taste crude drug can be added on the basis of above-mentioned formula, new formula is made up of the crude drug of following weight portion: Radix Astragali 20-35 part, Radix Codonopsis 20-30 part, Rhizoma Curcumae 30-40 part, Semen Lablab Album 15-25 part, raw pearl barley 15-20 part, Flos Lonicerae 10-20 part, Rhizoma Atractylodis Macrocephalae (parched) 5-10 part, Pericarpium Citri Reticulatae 5-10 part, Herba Epimedii 10-20 part, Retinervus Luffae Fructus 10-20 part, Ramulus Euonymi 5-10 part.Preferably, new formula is made up of the crude drug of following weight portion: the Radix Astragali 25 parts, Radix Codonopsis 22 parts, Rhizoma Curcumae 35 parts, Semen Lablab Album 20 parts, raw pearl barley 18 parts, Flos Lonicerae 15 parts, Rhizoma Atractylodis Macrocephalae (parched) 6 parts, Pericarpium Citri Reticulatae 6 parts, Herba Epimedii 15 parts, Retinervus Luffae Fructus 18 parts, Ramulus Euonymi 6 parts.
Raw material of the present invention is easy to get, formula unique, by the coordinated of each crude drug, obtains QI invigorating warming YANG, effect of detoxicating and resolving stagnation of pathogens.Each crude drug effect is as follows:
The Radix Astragali, Gan Wen, strongly invigorating primordial QI, benefiting qi and raising yang, arrogates to oneself the gas of spleen reinforcing lung especially, and in order to do the prosperous then QI and blood of lung temper, biochemical active, zang-fu differentiation is moistened foster.
Radix Codonopsis, Gan Ping, invigorating the spleen and replenishing QI, promotes the production of body fluid and nourishes blood, for deficiency of QI in middle-JIAO, lung qi virtual loss, consumption of body fluid caused by febrile disease and thirsty, the blood deficiency and yellow complexion and dizzy nervous of breathing hard, the most applicable to cold deficiency syndrome.
Rhizoma Curcumae, pungent, temperature, bitter, there is effect of removing blood stasis, promoting the circulation of QI to relieve pain, for the amenorrhea stomachache Ji lumps in the chest and abdomen of caused by energy stagnation and blood stasis, can be used for eating and drinking without temperance simultaneously, spleen fortune is not normal caused stagnantly not to be changed, the card of distension and fullness in the abdomen pain.
Semen Lablab Album, sweet, tepor, has effect of invigorating the spleen for eliminating dampness.For insufficiency of the spleen have wet, fatigue and asthenia, anorexia and loose stool or have loose bowels and the bet of women's insufficiency of the spleen turbid damp, leukorrhagia and heat-damp in summer vomiting and diarrhoea etc.
Raw pearl barley, sweet, light, there is effect of promoting diuresis to eliminate damp pathogen, spleen invigorating, eliminating impediment, clearing away heat and discharging pus, for dysuria, edema, beriberi and diarrhea due to hypofunction of the spleen, rheumatic arthralgia, muscle arteries and veins contraction anxious also energy clearing away heat and discharging pus can be treated, treatment abscess of internal organ.
Flos Lonicerae, sweet, cold, there is heat-clearing and toxic substances removing, effect of wind-heat dissipating, for carbuncle furuncle. sore throat, erysipelas, toxic-heat and blood stasis, anemopyretic cold, epidemic febrile disease is generated heat.
Rhizoma Atractylodis Macrocephalae (parched), sweet, bitter, temperature, has effect that air making-up and spleen enlivening, dampness diuretic, hidroschesis are antiabortive, for deficiency-weakness of spleen-QI, anorexia and loose stool caused by dysfunction of the spleen in transportation and transformation, distension and fullness in the abdomen, lassitude and insufficiency of the spleen can not transporting, water-damp retention and be the diseases such as phlegm retention edema.
Pericarpium Citri Reticulatae, pungent, bitter, temperature, have in regulating the flow of vital energy, adjusting, dampness, the effect of reducing phlegm, can be used for the abdominal distention uncomfortable in chest caused by the cards such as the distension and fullness in the abdomen caused by stagnation of QI in spleen and stomach, belch, nausea and vomiting and turbid damp obstructing in middle-JIAO, indigestion and loss of appetite asthenia, large loose stool be thin, thick fur is greasy and the card such as phlegm dampness obstruction pattern, impairment of dispersing and descending function of the lung, cough with copious phlegm QI rising in reverse order.
Herba Epimedii, acrid, sweet, warm, have effect of kidney-replenishing, bone and muscle strengthening, wind-damp dispelling, and for impotence and seminal emission, muscles and bones flaccidity is soft, rheumatic arthralgia, numbness contracture, climacteric hypertension.
Retinervus Luffae Fructus, sweet, flat, there is dredging collateral, invigorate blood circulation, the effect of dispeling the wind, for arthralgia pain contracture, distending pain in the chest and hypochondrium, galactostasis.
Ramulus Euonymi, has another name called winged euonymus, and bitter in the mouth is cold in nature, has blood circulation promoting and blood stasis dispelling, and effect of dredge the meridian passage cures mainly the traumatic injury that the passages through which vital energy circulates stasis of blood is stagnant, delay of menstrual cycle, the stagnant stomachache of the stasis of blood, and the disease such as rheumatic arthralgia.
The above-mentioned Chinese medicine composition of the present invention, can make the dosage forms such as tablet, capsule, granule, pill as required.The moulding process preparing different dosage form only used is different with adjuvant, effective ingredient or the same, and therapeutic effect is also identical or approximate.The adjuvant of tablet can be selected from starch, microcrystalline Cellulose, pregelatinized Starch, lactose, Icing Sugar, carboxymethylstach sodium, carmethose, hypromellose, polyvinylpolypyrrolidone, PVP K30, Pulvis Talci, magnesium stearate etc.; The adjuvant of capsule can be selected from starch, microcrystalline Cellulose, pregelatinized Starch, lactose, Icing Sugar, carboxymethylstach sodium, carmethose, hypromellose, polyvinylpolypyrrolidone, PVP K30, Pulvis Talci, magnesium stearate etc.; The adjuvant of granule can be selected from cane sugar powder, dextrin and correctives etc.; The adjuvant of pill can be selected from starch, microcrystalline Cellulose, pregelatinized Starch, dextrin, carboxymethylstach sodium, PVP K30, hypromellose etc.
Each crude drug directly simply can be worn into fine powder and use by above-mentioned Chinese medicine composition, also can prepare according to additive method.The invention provides a kind of above-mentioned preferred preparation method improving the cachectic Chinese medicine composition of intestinal cancer, comprise the following steps:
(1) each crude drug is mixed, extract 3 times with the alcoholic solution of 60 ~ 85wt%, merge extractive liquid;
(2) extracting solution is condensed into clear paste or thick paste, dry, get dry extract;
(3) dried cream powder is broken into fine powder, adds the adjuvant of survival dose, obtain Chinese medicine composition.
In above-mentioned steps (1), the consumption of alcoholic solution is 6 ~ 10 times of crude drug gross mass.
In above-mentioned steps (1), each time of extracting is 1-2 hour.
In above-mentioned steps (2), clear paste or the relative density of thick paste at 60 DEG C ~ 85 DEG C are 1.20 ~ 1.40.
In above-mentioned steps (3), corresponding survival dose adjuvant can be added according to required dosage form.
Several kinds of Chinese medicinal materials that Chinese medicine composition of the present invention is selected, by their collaborative onset play good integrally-regulated, improve cachexia amyotrophy, suppress muscle protein to decompose, to alleviate and opposing cachexia body weight Progressive symmetric erythrokeratodermia declines, improves life in patients, extends the effects such as survival of patients phase, intervening intestinal cancer cachexia for prevention has good effect.
Traditional Chinese medicine composite of the present invention is easy to get, and preparation method is simple, is convenient to suitability for industrialized production.
Accompanying drawing explanation
Fig. 1 is A group gastrocnemius muscle fiber HE colored graph;
Fig. 2 is B group gastrocnemius muscle fiber HE colored graph;
Fig. 3 is C group gastrocnemius muscle fiber HE colored graph.
Detailed description of the invention
Be further detailed the present invention below by specific embodiment, following explanation is only exemplary, does not limit its content.If no special instructions, following raw material medicaments is weight portion.
Embodiment 1
Traditional Chinese medicine composition formula is: the Radix Astragali 25 parts, Radix Codonopsis 22 parts, Rhizoma Curcumae 35 parts, Semen Lablab Album 20 parts, raw pearl barley 18 parts, Flos Lonicerae 15 parts, Rhizoma Atractylodis Macrocephalae (parched) 6 parts, Pericarpium Citri Reticulatae 6 parts.
Preparation method is:
(1) by the Radix Astragali, Radix Codonopsis, Rhizoma Curcumae, Semen Lablab Album, raw pearl barley, Flos Lonicerae, Rhizoma Atractylodis Macrocephalae (parched), Pericarpium Citri Reticulatae mixing, with the ethanol extraction 3 times of their 8 quality 80wt% doubly, each 1.5 hours, merge extractive liquid;
(2) extracting solution being condensed into relative density at 80 DEG C is clear paste or the thick paste of 1.20 ~ 1.40, dry, gets dry extract;
(3) dried cream powder is broken into fine powder, adds the adjuvant of survival dose, Chinese medicine composition is made the dosage forms such as tablet, capsule, granule, pill.
Embodiment 2
Traditional Chinese medicine composition formula is: the Radix Astragali 20 parts, Radix Codonopsis 30 parts, Rhizoma Curcumae 30 parts, Semen Lablab Album 25 parts, raw pearl barley 15 parts, Flos Lonicerae 20 parts, Rhizoma Atractylodis Macrocephalae (parched) 5 parts, Pericarpium Citri Reticulatae 10 parts.Preparation method is with embodiment 1.
Embodiment 3
Traditional Chinese medicine composition formula is: the Radix Astragali 35 parts, Radix Codonopsis 20 parts, Rhizoma Curcumae 40 parts, Semen Lablab Album 15 parts, raw pearl barley 20 parts, Flos Lonicerae 10 parts, Rhizoma Atractylodis Macrocephalae (parched) 10 parts, Pericarpium Citri Reticulatae 5 parts.Preparation method is with embodiment 1.
Embodiment 4
Traditional Chinese medicine composition formula is: the Radix Astragali 25 parts, Radix Codonopsis 22 parts, Rhizoma Curcumae 35 parts, Semen Lablab Album 20 parts, raw pearl barley 18 parts, Flos Lonicerae 15 parts, Rhizoma Atractylodis Macrocephalae (parched) 6 parts, Pericarpium Citri Reticulatae 6 parts, Herba Epimedii 15 parts, Retinervus Luffae Fructus 18 parts, Ramulus Euonymi 6 parts.
Preparation method is:
(1) by the Radix Astragali, Radix Codonopsis, Rhizoma Curcumae, Semen Lablab Album, raw pearl barley, Flos Lonicerae, Rhizoma Atractylodis Macrocephalae (parched), Pericarpium Citri Reticulatae, Herba Epimedii, Retinervus Luffae Fructus, Ramulus Euonymi mixing, with the ethanol extraction 3 times of their 8 quality 80wt% doubly, each 1.5 hours, merge extractive liquid;
(2) extracting solution being condensed into relative density at 80 DEG C is clear paste or the thick paste of 1.20 ~ 1.40, dry, gets dry extract;
(3) dried cream powder is broken into fine powder, adds the adjuvant of survival dose, Chinese medicine composition is made the dosage forms such as tablet, capsule, granule, pill.
Embodiment 5
Traditional Chinese medicine composition formula is: the Radix Astragali 20 parts, Radix Codonopsis 30 parts, Rhizoma Curcumae 30 parts, Semen Lablab Album 25 parts, raw pearl barley 15 parts, Flos Lonicerae 20 parts, Rhizoma Atractylodis Macrocephalae (parched) 5 parts, Pericarpium Citri Reticulatae 10 parts, Herba Epimedii 10 parts, Retinervus Luffae Fructus 20 parts, Ramulus Euonymi 5 parts.Preparation method is with embodiment 4.
Embodiment 6
Traditional Chinese medicine composition formula is: the Radix Astragali 35 parts, Radix Codonopsis 20 parts, Rhizoma Curcumae 40 parts, Semen Lablab Album 15 parts, raw pearl barley 20 parts, Flos Lonicerae 10 parts, Rhizoma Atractylodis Macrocephalae (parched) 10 parts, Pericarpium Citri Reticulatae 5 parts, Herba Epimedii 20 parts, Retinervus Luffae Fructus 10 parts, Ramulus Euonymi 10 parts.Preparation method is with embodiment 4.
Chinese medicine composition of the present invention has good improvement result for lung cancer cachexia, in order to verify its curative effect, exemplifies following experiment.
1, laboratory animal
The healthy BALB/c mouse 30 of SPF level, in 5 week age, weight (20 ± 2) g, male, inbred line, in Beijing, Fukang Biotechnology Ltd. of China buys, the quality certification number: SCXK (capital) 20090004.Raise in the IVC independent air-blowing-returning purification cage of Shandong Traditional Chinese Medicine University's animal experimental center.Rearing conditions is: room temperature 22-28 DEG C, relative humidity 40%-70%.
C26 colon carcinoma solid tumor tumor source Mus, male, be purchased from Beijing Li Keaida biotechnology consultative centre.
2, Experimental agents
Chinese medicine composition prepared by embodiment 4.
3, method for establishing model
Choose BALB/c mouse 20, in an aseptic environment by right for mice axil skin routine disinfection, in right axil subcutaneous vaccination C26 colon cancer cell suspension, only, stochastic averagina is divided into model control group (B) and Chinese medicine intervention group (C) to 0.2ml/; Separately get BALB/c mouse 10 only as Normal group (A group), with injection location normal saline, be 0.2ml/.
The same day is inoculated in laboratory animal oxter, and namely C group starts administration, and medicine is Chinese medicine composition prepared by embodiment 4, dosage is crude drug 36g/ kilogram, by crude drug water dissolution during administration, carry out gavage according to 0.2mL/10g weight, other each group to wait dosage normal saline gavage.Every day 2 times, continue medication 15 days.Observe Mice Body quality, food ration, amount of drinking water, tumor volume, tumor weight, the change of muscle quality.
4, index observing
4.1 first weights
Recording laboratory animal is weight just, and weighs the weight of every mice during 9:00-10:00 in morning every day.
4.2 food ration
Morning every day, 9:00-10:00 quantitatively gave each mouse feed, weighed feedstuff surplus after 24h, and feedstuff intake=first 1 day feed relative-records forage volume in 2 days.
4.3 amount of drinking water
Morning every day, 9:00-10:00 measured every mice amount of drinking water, calculated the drinking-water consumption of mice, and drinking-water intake=first 1 day drinking-water weight records amount of drinking water in-the second day.
4.4 tumor cubings
Subcutaneous vaccination starts the long and short diameter measuring tumor with vernier cursor on the 4th day, tumor volume (V)=ab
2/ 2.Wherein a, b represent the long and short diameter of tumor.
4.5 tumor weights
Administration after 15 days mice claim its weight, then complete stripping tumor tissue, claim tumor weight.
4.6 weight rates of descent
Meter record laboratory animal, except tumor end-body quality, calculates weight rate of descent.
4.7 gastrocnemius quality
Get that mice is prostrate to be positioned on animal operating table, the skin of shank shin front portion, subcutaneous tissue and deep fascia are cut, not damaged peels off calf gastrocnemius and extensor digitorum longus, claims its quality.
5, experimental result
5.1, general state
After model is set up, B group is model control group, and mice activity is slow, and neck back wool is erect, color is gloomy, and have obscission, tumor obviously increases, and weight declines obviously; C group is Chinese medicine intervention group, and mice state comparatively model control group makes moderate progress, and fur is erect but without obvious obscission, hair color obfuscation but totally and slightly gloss.Normal group A group mice state is good all the time, and fur is fine and close, smooth, moist all the time, and hair color is totally glossy, is quick on the draw.
5.2 food rations, amount of drinking water change
Table 1 mice food ration, amount of drinking water change
| Group | Food ration | Amount of drinking water |
| A group | 3.19±0.27 | 3.20±0.42 |
| B group | 2.20±0.58 ** | 2.36±0.56 ** |
| C group | 2.43±0.60 ** | 2.53±0.50 ** |
Note: compare with A group,
*p<0.05,
*p<0.01.
5.3 Mice Body Quality Down rates
The initial weight of table 2 experiment mice, remove tumor weight, weight rate of descent
| Group | Initial weight | Remove tumor weight | Weight rate of descent |
| A group | 21.08±0.99 | — |
| B group | 20.94±0.97 | 15.07±1.04 | 28.03％ |
| C group | 20.78±0.86 | 16.84±0.53 △ | 18.96％ △ |
Note: compare with A group,
*p<0.05,
*p<0.01; Compare with B group,
△p<0.05,
△ △p<0.01.
Compare with B group, C group goes the heavy obviously increase of tumor body constitution, statistical analysis has significant difference (P<0.05), and the weight rate of descent of C group mice is significantly less than B group, and statistical analysis has significant difference (P<0.05).
5.4 gross tumor volume changes
Table 3 lotus tumor group mouse tumor change in volume
| Group | 9th day tumor volume after inoculation | Final tumor volume |
| A group | — | — |
| B group | 1555.35±143.11 | 5579.29±300.24 |
| C group | 1028.41±141.23 | 3114.57±364.53 △△ |
Note: compare with B group,
Δp<0.05,
Δ Δp<0.01.
Compare with B group, C group gross tumor volume obviously reduces, and statistical analysis has significant difference (P<0.01).
5.5 tumour inhibiting rate
Table 4 tumour inhibiting rate
| Group | Tumor weight | Tumour inhibiting rate |
| A group | — | — |
| B group | 8.97±0.30 | — |
| C group | 6.63±0.55 △△ | 26.09％ |
Note: compare with B group,
△p<0.05,
△ △p<0.01.
Weigh each group of mouse tumor, calculate tumour inhibiting rate, the average tumor of tumour inhibiting rate (%)=(model group average tumor weight-intervention group average tumor weight)/model group heavy × 100%.Compare with B group, C group tumor weight obviously reduces, and statistical analysis has significant difference (P<0.01).5.6 muscle quality
Table 5 muscle quality
Note: compare with A group,
*p<0.05,
*p<0.01.Compare with B group,
△p<0.05.
Compare with A group, each group mouse muscle gross weight obviously reduces, and statistical analysis has significant difference (P<0.01).Compare with B group, C group mouse muscle gross weight obviously increases, and statistical analysis has significant difference (P<0.05).
5.7 gastrocnemius fibre morphology observed results
Gastrocnemius muscle fiber cross-sectional slices, can be observed A group mice gastrocnemius lines through HE dyeing clear, Skeletal Muscle Cell arrangement is tight, rounded or polygon, and nucleus multidigit is in cell periphery; B group mice gastrocnemius myocyte arranges loose, and myofibroblasts reduces, and intercellular substance increases, and myofibroblasts difference in size is larger; C group mice myenteron myocyte arranges relative close, and myofibroblasts slightly reduces.
5.8 gastrocnemius myofibroblasts transverse diameters are measured
Table 6 gastrocnemius myofibroblasts transverse diameter
| Group | Myofibroblasts transverse diameter |
| A group | 220.50±8.19 |
| B group | 79.14±2.238 ** |
| C group | 128.00±4.04 **△△ |
Note: compare with A group,
*p<0.05,
*p<0.01; Compare with B group,
△p<0.05,
△ △p<0.01
Compared with A, B group mice gastrocnemius myofibroblasts transverse diameter significantly reduces, and statistical analysis has significant difference (P<0.01), illustrates that the gastrocnemius of cachexia model exists obvious atrophy phenomenon.Compare with B group, C group mice gastrocnemius myofibroblasts transverse diameter significantly increases, and statistical analysis has significant difference (P<0.01).
Further, in order to verify different formulations Chinese medicine composition between the difference of curative effect, carry out following contrast test.
1, laboratory animal and grouping:
BALB/c mouse 60, is equally divided into 6 groups, no difference of science of statistics between each group mice.Wherein one group is Normal group, and one group is model control group, and other four groups is experimental group.
C26 colon carcinoma solid tumor tumor source Mus 2.
2, experimental technique
2.1 inoculations according to above-mentioned same method, model control group and experimental mice being carried out tumor cell, set up colon cancer cachexia mouse model, Normal group mouse inoculation saline solution in contrast.
2.2 application method
The same day is inoculated in laboratory animal oxter, and Normal group and model control group, with normal saline 0.2mL/10g weight gavage, every day 2 times, continue 15 days; Each experimental group carries out gavage with the Chinese medicine composition of embodiment 1,2,4,5 according to crude drug 36g/ kilogram respectively, every day 2 times, continues medication 15 days.
The weight of 2.3 observations each group of mice, food ration, amount of drinking water, tumor volume, tumor weight, the features such as muscle quality, line item of going forward side by side.
3, experimental result
3.1 mice states
In whole experimentation, Normal group mice is in good condition, and diet, amount of drinking water are without significant change, and fur is fine and close, smooth, moist all the time, and hair color is totally glossy, is quick on the draw, frequent activity, and weight increased gradually along with the time.
In whole experimentation, the activity of model control group mice is slow, and neck back wool is erect, color is gloomy, and have obscission, tumor obviously increases, and weight declines obviously.
In whole experimentation, experimental mice fur is erect but without obvious obscission, hair color obfuscation but totally and slightly gloss, and amount of drinking water of ingesting, activity reduce, but comparatively model group makes moderate progress.
3.2 weight rates of descent
Table 7 weight rate of descent
| Group | Initial body constitution weight | Remove tumor body constitution weight | Weight rate of descent |
| Embodiment 1 | 21.59 | 16.77±0.78 | 22.34％ |
| Embodiment 2 | 21.42 | 16.04±0.89 | 25.12％ |
| Embodiment 4 | 21.31 | 17.29±1.01 | 18.86％ |
| Embodiment 5 | 21.58 | 16.89±0.67 | 21.73％ |
| Model control group | 21.73 | 15.54±0.52 | 28.49％ |
3.3 mouse tumor change in volume situations
Table 8 respectively group experiment mice gross tumor volume change
| Group | 9th day tumor volume after inoculation | Final tumor volume |
| Embodiment 1 | 1249±132.12 | 4025.38±325.12 |
| Embodiment 2 | 1376±125.28 | 4888.21±368.29 |
| Embodiment 4 | 1022.12±121.35 | 3102.33±365.44 |
| Embodiment 5 | 1209±128.14 | 4023.56±389.12 |
| Model control group | 1556.55±140.01 | 5587.31±302.45 |
3.4 tumour inhibiting rate
Table 9 tumour inhibiting rate ((X ± S, g)
| Group | Tumor weight | Tumour inhibiting rate |
| Embodiment 1 | 7.48±0.35 | 17.98％ |
| Embodiment 2 | 8.19±0.33 | 10.20％ |
| Embodiment 4 | 6.60±0.31 | 27.63％ |
| Embodiment 5 | 7.43±0.34 | 18.53％ |
| Model control group | 9.12±0.51 | -- |
3.5 muscle quality
Table 10 muscle quality
| Group | Gastrocnemius muscle weight | Extensor digitorum longus weight |
| Embodiment 1 | 0.057±0.007 | 0.004284±0.001901 |
| Embodiment 2 | 0.055±0.004 | 0.004257±0.001921 |
| Embodiment 4 | 0.059±0.006 | 0.004338±0.001945 |
| Embodiment 5 | 0.057±0.005 | 0.004289±0.001933 |
| Normal group | 0.090±0.021 | 0.005626±0.002490 |
| Model control group | 0.053±0.005 | 0.004225±0.001901 |
3.6 gastrocnemius myofibroblasts transverse diameters are measured
Table 11 gastrocnemius myofibroblasts transverse diameter
| Group | Myofibroblasts transverse diameter |
| Embodiment 1 | 108±3.35 |
| Embodiment 2 | 92±2.14 |
| Embodiment 4 | 128.54±3.78 |
| Embodiment 5 | 110±3.55 |
| Normal group | 220.15±8.29 |
| Model control group | 78.28±2.23 |
4, conclusion
As can be seen from above experiment, the Chinese medicine composition of the embodiment of the present invention 1,2,4,5 all has good improvement result to colon cancer cachexia, and the effect of the Chinese medicine composition of embodiment 4 is best.
Claims (9)
1. improve the cachectic Chinese medicine composition of intestinal cancer, it is characterized in that the crude drug comprising following weight portion: Radix Astragali 20-35 part, Radix Codonopsis 20-30 part, Rhizoma Curcumae 30-40 part, Semen Lablab Album 15-25 part, raw pearl barley 15-20 part, Flos Lonicerae 10-20 part, Rhizoma Atractylodis Macrocephalae (parched) 5-10 part, Pericarpium Citri Reticulatae 5-10 part.
2. Chinese medicine composition according to claim 1, is characterized in that the crude drug comprising following weight portion: the Radix Astragali 25 parts, Radix Codonopsis 22 parts, Rhizoma Curcumae 35 parts, Semen Lablab Album 20 parts, raw pearl barley 18 parts, Flos Lonicerae 15 parts, Rhizoma Atractylodis Macrocephalae (parched) 6 parts, Pericarpium Citri Reticulatae 6 parts.
3. Chinese medicine composition according to claim 1 and 2, is characterized in that the crude drug also comprising following weight portion: Herba Epimedii 10-20 part, Retinervus Luffae Fructus 10-20 part, Ramulus Euonymi 5-10 part.
4. Chinese medicine composition according to claim 3, is characterized in that the crude drug also comprising following weight portion: Herba Epimedii 15 parts, Retinervus Luffae Fructus 18 parts, Ramulus Euonymi 6 parts.
5. the Chinese medicine composition according to any one of claim 1-4, is characterized in that: dosage form is tablet, capsule, granule or pill.
6. a preparation method for the cachectic Chinese medicine composition of improvement intestinal cancer according to any one of claim 1-4, is characterized in that comprising the following steps:
(1) each crude drug is mixed, extract 3 times with the alcoholic solution of 60 ~ 85wt%, merge extractive liquid;
(2) extracting solution is condensed into clear paste or thick paste, dry, get dry extract;
(3) dried cream powder is broken into fine powder, adds the adjuvant of survival dose, obtain Chinese medicine composition.
7. preparation method according to claim 6, is characterized in that: in step (1), and the consumption of alcoholic solution is 6 ~ 10 times of crude drug gross mass.
8. preparation method according to claim 6, is characterized in that: in step (1), each 1-2 hour.
9. preparation method according to claim 6, is characterized in that: in step (2), and clear paste or the relative density of thick paste at 60 DEG C ~ 85 DEG C are 1.20 ~ 1.40.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115317543A (en) * | 2022-09-13 | 2022-11-11 | 复旦大学附属金山医院(上海市金山区眼病防治所、上海市金山区核化伤害应急救治中心) | Traditional Chinese medicine composition for treating liver cancer cachexia and application thereof |
| CN115317543B (en) * | 2022-09-13 | 2023-05-23 | 复旦大学附属金山医院(上海市金山区眼病防治所、上海市金山区核化伤害应急救治中心) | Traditional Chinese medicine composition for treating liver cancer cachexia and application thereof |
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