CN105211261B - A kind of tuna antistaling agent and its application method - Google Patents
A kind of tuna antistaling agent and its application method Download PDFInfo
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- CN105211261B CN105211261B CN201510532248.7A CN201510532248A CN105211261B CN 105211261 B CN105211261 B CN 105211261B CN 201510532248 A CN201510532248 A CN 201510532248A CN 105211261 B CN105211261 B CN 105211261B
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Abstract
The invention discloses a kind of tuna antistaling agent and its application method, the tuna antistaling agent is grouped as by the group of following parts by weight: 5~10 parts of marine low temperature lysozyme, 3~8 parts of nisin, 15~20 parts of modified alta-mud, 300 parts of water.Tuna antistaling agent of the invention is using marine low temperature lysozyme and nisin as main active, using modified alta-mud as absorption carrier, utilize bentonitic adhesive property, the modified alta-mud film of marine low temperature lysozyme and nisin will be adsorbed on tuna surface, effectively reduce the loss of active constituent in antistaling agent, extend the preservation time of tuna, simultaneously, due to bentonitic water retention property, it can prevent tuna from causing the loss of intracellular nutrient because of dehydration, ensure that the freshness of tuna.
Description
Technical field
The present invention relates to technical field of aquatic product preservation more particularly to a kind of tuna antistaling agent and its application methods.
Background technique
Tuna -- like Fishes are the general name of several category fish in Osteichthyes Perciformes mackerel section fish with cuirass, also known as sturgeon
Fish, tuna fish are a kind of height migration fishes for living in ocean waters at the middle and upper levels, mainly respectively the Pacific Ocean, the Atlantic Ocean,
The torrid zone, the wide waters in subtropical zone and temperate zone of the Indian Ocean.Common Tuna -- like Fishes have 5 categories, and 17 kinds.It mainly include long fin
Tuna, Atlantic tuna, yellowfin tuna, big-eye tunas, bluefin tuna, stripped tuna etc..Tuna is Fresh & Tender in Texture, egg
White matter content is very high, and biological value is up to 90, and amino acid cooperation is superior；Mostly not rich in the biologically active height such as DHA, EPA
Saturated fatty acid can promote brain development, effectively prevent cardiovascular and cerebrovascular disease；Meanwhile methionine, taurine, minerals and dimension
Raw cellulose content is abundant, is the green non-pollution food that international Nutrition Association is recommended.
Tuna belongs to abyssal fishes, generally needs about 30 days from capturing market, therefore, preservation technology is to golden rifle on ship
For this high level economic fish of fish, it appears particularly important.Currently, the most widely used preservation mode of seafood freshing is ice
Hiding, iced storage can slow down the breeding of microorganism in fish body, and the activity of enzyme in fish body is allowed to be suppressed, thus long-time preservation fish,
But iced storage will lead to the disadvantages of juice loss and nutritional ingredient reduce when fish meat protein is denaturalized, thaws.In addition to iced storage is
Except the preservation by low temperature of representative, applied to the preservation technology of marine product, there are also fresh chemically, irradiated targets, biological preservations at present
Deng wherein low in cost, easy to spread and of good preservation effect using chemical preservative.In recent years, with people's environmental consciousness
It improves and the development of preservation technology, the safety of fresh chemically gradually causes the worry of people.Using bio-preservative to marine products
Product carry out the fresh-keeping adverse effect that can reduce chemical synthesis antistaling agent to human health, and effectively prevent the anti-medicine of pathogen
Property, it is considered to be the most promising developing direction of food additives research field is the new and effective marine product biological antiseptic of exploitation
The important channel of antistaling agent.
Chinese patent publication No. CN102860568A, date of publication disclose a kind of aquatic product fresh keeping agent on January 9th, 2013,
The antistaling agent is dissolved in water by weight components and configures: calgon, sodium tripolyphosphate, sodium pyrophosphate three's mass ratio
For 10:2:1, and three's total weight accounts for the 50~57% of antistaling agent total weight, sodium alginate 15~20%, and ascorbic acid 5~
10%, glycerol 2~10%.The antistaling agent improves the water-retaining property of aquatic products using phosphate as water-retaining agent, reduces aquatic products water
On the one hand the loss divided, but the phosphate content in the antistaling agent at least 50%, excessive phosphate can be such that aquatic products generate
Offending metal astringent taste, and musculature is made to become coarse, the flavor and mouthfeel of aquatic products are influenced, on the other hand, consumer
It is edible excessively to add phosphatic aquatic products, blood clotting can be caused, caused harm to the human body.
Summary of the invention
It is an object of the invention to overcome existing tuna using safety issue existing for chemical preservative, one kind is provided
Tuna antistaling agent, the antistaling agent is using marine low temperature lysozyme and nisin as main active, with bentone
Soil will be adsorbed with the modification of marine low temperature lysozyme and nisin using bentonitic adhesive property for absorption carrier
Bentonite film effectively reduces the loss of active constituent in antistaling agent on tuna surface, when extending the preservation of tuna
Between, simultaneously as bentonitic water retention property, can prevent tuna from causing the loss of intracellular nutrient because of dehydration, guarantee
The freshness of tuna.
The present invention also provides a kind of application methods of tuna antistaling agent.
The specific technical proposal of the invention is: a kind of tuna antistaling agent, be grouped as by the group of following parts by weight: ocean is low
5~10 parts of warm lysozyme, 3~8 parts of nisin, 15~20 parts of modified alta-mud, 300 parts of water.
Marine low temperature lysozyme is a kind of novel lysozyme being separated to from the metabolite of ocean extremophile microorganism, tool
Have the characteristics that thermophilic low temperature, anti-oxidant and bacteriolyze compose it is wide, relatively be suitble to deepfreeze fish it is fresh-keeping, nisin is lactic acid
A kind of peptide material that streptococcus generates, can inhibit most of gram-positive bacteriums, and have strongly to the spore of bacillus
Inhibiting effect.The present invention utilizes bentonitic absorption property and adhesive property, will be adsorbed with marine low temperature lysozyme and lactic acid
The modified alta-mud of streptostacin, film effectively reduce the loss of antistaling agent active constituent on tuna surface, extend guarantor
It hides the time, meanwhile, in conjunction with the good water retention property of bentonite, so that fish body is kept activated state, to ensure that the new of tuna
Freshness.Additionally, due to bentonite adsorption performance, fishy smell can also be effectively adsorbed, reduces the fishlike smell of tuna.
Preferably, the modified alta-mud is prepared by the following steps:
(1) bentonite original soil crushed, dried, after grinding, crossing 250~300 meshes；
(2) bentonite after sieving is mixed with hydrochloric acid solution 1:3~4 in mass ratio that mass fraction is 13~18%,
It is placed in microwave applicator and heats；
(3) after heating, mixture is filtered, after filter cake is freeze-dried, 30~45min of activation is swollen to get modification
Profit soil.
The present invention uses HCl treatment bentonite, some non-adsorbent impure minerals in bentonite can be decomposed, be mentioned
The high content of tool adsorptivity montmorillonite.Meanwhile the H of minor radius high activity+Easily replace the interchangeability sun between montmorillonite crystal layer
Ion K+、Na+、Ca+、Mg2+Plasma increases surface area to change surface potential, to increase bentonitic suction
Attached performance.
Preferably, the activation temperature is 300~350 DEG C.High-temperature roasting can make bentonite lose part itself contain
Some absorption water, and the air inside bentonite is escaped, to form many tiny gaps inside bentonite, increase
Specific surface area enhances bentonitic adsorption capacity.
Preferably, the microwave applicator power is 800~900W, 6~8min of heating time.Using microwave heating,
Heating time can be shortened, it is energy saving.
A kind of application method of tuna antistaling agent, comprising the following steps:
(1) it after cleaning up fresh tuna, is soaked in the tuna antistaling agent, then takes out and drain, and
Recycle the tuna antistaling agent flowed down；
(2) the tuna vacuum packaging after draining, is placed in 3~5 DEG C of refrigerations.
Fresh tuna is immersed in antistaling agent, the effective component due to bentonitic adhesive property, in antistaling agent
Can effectively film not easily runed off on tuna surface.Meanwhile refrigerated storage temperature is 5~10 DEG C, at this temperature, marine low temperature
Lysozyme activity also with higher.
Preferably, the soaking time is 4~6min.Soaking time influences the adsorbance of antistaling agent, and the time is too short, inhales
Attached antistaling agent is very little, influences fresh-keeping effect.
Preferably, the tuna antistaling agent and tuna 1:2 in mass ratio~3 are used.Tuna antistaling agent has
Composition influence fresh-keeping effect is imitated, tuna is too many, and tuna antistaling agent is very little, and fresh-keeping effect is with regard to poor.
The beneficial effects of the present invention are: (1) present invention using modified alta-mud as absorption carrier, utilizes bentonitic caking property
Can, by the modified alta-mud film for being adsorbed with marine low temperature lysozyme and nisin on tuna surface, effectively reduce
The loss of active constituent, extends the preservation time of tuna in antistaling agent；(2) due to the good water retention property of bentonite,
It can prevent tuna from causing the loss of intracellular nutrient because of dehydration, ensure that the freshness of tuna；(3) present invention provides
Tuna antistaling agent, be free of chemical component, safety and environmental protection.
Specific embodiment
Below by specific embodiment, technical scheme is described further.
The marine low temperature lysozyme molecular weight that the present invention uses is ground for 39KD by China Aquatic Science Research Institute's Huanghai Sea aquatic products
Study carefully and provided, nisin is by China silver as biological Co., Ltd provides.
If not other raw materials of the present invention and equipment are refered in particular to, it is commercially available or commonly used in the art,
Method in embodiment is unless otherwise instructed the conventional method of this field.
Embodiment 1
A kind of tuna antistaling agent, is grouped as by the group of following parts by weight: 5 parts of marine low temperature lysozyme, streptococcus lactis
3 parts, 15 parts of modified alta-mud, 300 parts of water of element.
Wherein, modified alta-mud is prepared by the following steps:
(1) bentonite original soil crushed, dried, after grinding, crossing 250 meshes；
(2) bentonite after sieving is mixed with the hydrochloric acid solution 1:3 in mass ratio that mass fraction is 13%, is placed in microwave
6min is heated in heater, the power of microwave applicator is 800W；
(3) after heating, mixture is filtered, after filter cake is freeze-dried, in 300 DEG C of activation 30min to get modification
Bentonite.
The application method of tuna antistaling agent made of being configured as said components, comprising the following steps:
(1) after cleaning up fresh tuna, it is soaked in 4min in tuna antistaling agent, then takes out and drains, and
The tuna antistaling agent flowed down is recycled, wherein tuna antistaling agent and tuna mass ratio are 1:2；
(2) the tuna vacuum packaging after draining, is placed in 5 DEG C of refrigerations.
Embodiment 2
A kind of tuna antistaling agent, is grouped as by the group of following parts by weight: 8 parts of marine low temperature lysozyme, streptococcus lactis
5 parts, 18 parts of modified alta-mud, 300 parts of water of element.
Wherein, modified alta-mud is prepared by the following steps:
(1) bentonite original soil crushed, dried, after grinding, crossing 300 meshes；
(2) bentonite after sieving is mixed with the hydrochloric acid solution 1:3.5 in mass ratio that mass fraction is 15%, is placed in micro-
7min is heated in wave heater, the power of microwave applicator is 850W；
(3) after heating, mixture is filtered, after filter cake is freeze-dried, in 320 DEG C of activation 40min to get modification
Bentonite.
The application method of tuna antistaling agent made of being configured as said components, comprising the following steps:
(1) after cleaning up fresh tuna, it is soaked in 5min in tuna antistaling agent, then takes out and drains, and
The tuna antistaling agent flowed down is recycled, wherein tuna antistaling agent and tuna mass ratio are 1:2.5；
(2) the tuna vacuum packaging after draining, is placed in 8 DEG C of refrigerations.
Embodiment 3
A kind of tuna antistaling agent, is grouped as by the group of following parts by weight: 10 parts of marine low temperature lysozyme, streptococcus lactis
8 parts, 20 parts of modified alta-mud, 300 parts of water of element.
Wherein, modified alta-mud is prepared by the following steps:
(1) bentonite original soil crushed, dried, after grinding, crossing 300 meshes；
(2) bentonite after sieving is mixed with the hydrochloric acid solution 1:4 in mass ratio that mass fraction is 18%, is placed in microwave
8min is heated in heater, the power of microwave applicator is 900W；
(3) after heating, mixture is filtered, after filter cake is freeze-dried, in 350 DEG C of activation 45min to get modification
Bentonite.
The application method of tuna antistaling agent made of being configured as said components, comprising the following steps:
(1) after cleaning up fresh tuna, it is soaked in 6min in tuna antistaling agent, then takes out and drains, and
The tuna antistaling agent flowed down is recycled, wherein tuna antistaling agent and tuna mass ratio are 1:3；
(2) the tuna vacuum packaging after draining, is placed in 10 DEG C of refrigerations.
By the tuna in embodiment 1, embodiment 2, embodiment 3, after refrigeration 30 days, takes out and carry out sensory evaluation, comment
Price card is quasi- as shown in table 1, and obtained evaluation result is as shown in table 2.
From table 2 it can be seen that it is fresh-keeping to tuna progress using tuna antistaling agent of the invention, it is refrigerated at 5~10 DEG C
It can at least save 30 days, illustrate that the present invention has good fresh-keeping, water conservation and antiseptic property, can guarantee tuna color
Under the premise of pool, vividness and form are not destroyed, the fresh keeping time of tuna is greatly prolonged.
1 sensory evaluation criteria of table
The Analyses Methods for Sensory Evaluation Results of each group tuna after table 2 refrigerates 30 days
Claims (5)
1. a kind of tuna antistaling agent, which is characterized in that be grouped as by the group of following parts by weight: marine low temperature lysozyme 5~10
Part, 3~8 parts of nisin, 15~20 parts of modified alta-mud, 300 parts of water；Modified alta-mud is prepared by the following steps:
(1) bentonite original soil crushed, dried, after grinding, crossing 250~300 meshes；
(2) bentonite after sieving is mixed with hydrochloric acid solution 1:3~4 in mass ratio that mass fraction is 13~18%, is placed in
It is heated in microwave applicator；
(3) heat after, mixture is filtered, after filter cake is freeze-dried, activate 30~45min, activation temperature be 300~
350 DEG C to get modified alta-mud.
2. a kind of tuna antistaling agent according to claim 1, which is characterized in that the microwave applicator power is 800
~900W, 6~8min of heating time.
3. a kind of application method such as the described in any item tuna antistaling agents of claim 1~2, which is characterized in that including with
Lower step:
(1) it after cleaning up fresh tuna, is soaked in the tuna antistaling agent, then takes out and drain, and recycle
The tuna antistaling agent flowed down；
(2) the tuna vacuum packaging after draining, is placed in 5~10 DEG C of refrigerations.
4. a kind of application method of tuna antistaling agent according to claim 3, which is characterized in that the soaking time is
4~6min.
5. a kind of application method of tuna antistaling agent according to claim 3, which is characterized in that the tuna is fresh-keeping
Agent and tuna 1:2 in mass ratio~3 are used.
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| CN109479948A (en) * | 2018-12-10 | 2019-03-19 | 湖北楚玉莱信克食品科技有限公司 | A kind of single-freeze fillets of ictalurus punctatus processing method for keeping fresh |
| CN109845807A (en) * | 2019-02-21 | 2019-06-07 | 福州宏东食品有限公司 | A kind of processing method of fish |
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| CN1476764A (en) * | 2003-07-16 | 2004-02-25 | �Ϻ���ͨ��ѧ | Fruit and evgetable fresh-keeping agent and its production method |
| CN101496534A (en) * | 2008-01-29 | 2009-08-05 | 郑州轻工业学院 | Composite biological bacteriostatic agent and method for controlling decomposition of cooled meat thereby cooperated with ultra-high pressure |
| CN101461414A (en) * | 2009-01-19 | 2009-06-24 | 湖南农业大学 | Compound biological preservative for cooling pork and fresh-keeping method |
| CN101779700A (en) * | 2010-03-25 | 2010-07-21 | 浙江大学 | Compound preservative for western-style sausages |
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