CN105210856B - A kind of seed selection of flue-cured tobacco sterile line new varieties and propagation method - Google Patents
A kind of seed selection of flue-cured tobacco sterile line new varieties and propagation method Download PDFInfo
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- CN105210856B CN105210856B CN201510729907.6A CN201510729907A CN105210856B CN 105210856 B CN105210856 B CN 105210856B CN 201510729907 A CN201510729907 A CN 201510729907A CN 105210856 B CN105210856 B CN 105210856B
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Abstract
The invention discloses a kind of seed selection of flue-cured tobacco sterile line new varieties and propagation method, methods described is originated using flue-cured tobacco cytoplasmic male sterile line MSG28 as Cytoplasmic male sterile genes, using MSG28 as female parent, it is recurrent parent with kind TZ 1, in the generation of continuous backcross 6 after hybridization, cultivate into novel fine cytoplasm male sterility line MSTZ 1；It is female parent with sterile line MSTZ 1, is male parent with maintainer TZ 1, outbreeding preserves sterile line MSTZ 1；The flue-cured tobacco sterile line MSTZ 1 of seed selection of the present invention has the advantages that sterility is stable, comprehensive resistance is strong, tobacco leaf outward appearance endoplasm is good, chemical composition is coordinated, and a new excellent sterile system germplasm is provided for flue-cured tobacco heterosis utilization research work.
Description
Technical field
The invention belongs to Tobacco Genetic Breeding technical field, and in particular to the seed selection of flue-cured tobacco cytoplasmic male sterile line and breeding
Method.
Background technology
Flue-cured tobacco has stronger Heterosis in terms of yield, disease resistance, breeding time, growth potential, but due to roasting
Tobacco interspecific hybridization is almost without obvious hybrid vigour in terms of quality of tobacco, and the work of flue-cured tobacco heterosis utilization is once baked
Cigarette breeder abandons.In recent years, because the sizing kind of seed selection in China's flue-cured tobacco breeding has breakthrough seldom, cause
National cigarette district main breed is single, and varietal resistance declines, the harm of pest and disease damage large area, China's cured tobacco production is caused serious
Loss.Flue-cured tobacco is due to stronger resistance hybrid vigour, and heterosis utilization cycle in breeding is short, instant effect spy
Point, domestic breeder starts to pay close attention to flue-cured tobacco heterosis utilization again again, assign it as a kind of breeding side quickly tackled
Method.
The performance of hybrid vigour is related to parent's genetic diversity, increases with being incremented by for parent's genetic distance.Therefore,
The flue-cured tobacco sterile line of seed selection genetic diversity, it is that flue-cured tobacco heterosis utilization is extremely important to expand the germ plasm resource that may be selected to utilize
Element task.
The content of the invention
The technical problems to be solved by the invention are, for need of the flue-cured tobacco heterosis utilization to sterile line genetic diversity
Ask there is provided a kind of seed selection of flue-cured tobacco sterile line new varieties and propagation method, excellent sterile system is provided for flue-cured tobacco heterosis utilization
Germplasm, widens the hereditary basis of flue-cured tobacco heterosis utilization, to overcome deficiency of the prior art.
Technical scheme：
A kind of selection of flue-cured tobacco sterile line new varieties：Using sterile line MSG28 as female parent, using TZ-1 as recurrent parent,
Sterile line new varieties MSTZ-1 is bred as by continuous backcross.
Foregoing selection, is comprised the following steps：
（1）Hybridization：Using sterile line MSG28 as female parent, using TZ-1 as male parent, hybridization obtains F1Seed；
（2）Continuous backcross：With F1For female parent, using TZ-1 as recurrent parent, backcrossing obtains BC1F1Seed；Using TZ-1 as samsara
Parent, and backcross progeny generation of continuous backcross 5 again, until obtaining BC6F1Seed, as sterile line new varieties MSTZ-1.
The propagation method of above-mentioned flue-cured tobacco sterile line new varieties：Using sterile line new varieties MSTZ-1 as female parent, using TZ-1 as
Male parent, breeds MSTZ-1.
The propagation method of foregoing flue-cured tobacco sterile line new varieties, is comprised the following steps：
（1）Plantation：Using MSTZ-1 as female parent, using TZ-1 as male parent, the sowing of the Parent same period, the same period transplant, male parent and mother
This is according to 1:Plant at 4 column number proportion intervals；
（2）Roguing:When cigarette strain is buddingged, hybrid strain is strictly gone, weak plant is gone, goes diseased plant, and thoroughly remove cigarette wooden fork, pressed
Type strain bagging is selected according to parent's standard；
（3）Artificial pollination：When MSTZ-1 corollas top red occurs without opening, exposed using manual method female
Stamen, is then evenly coated in TZ-1 pollen on the column cap of gynoecium；
（4）Timely sowing：According to the sequencing of hybridization pollination, ripe a collection of, harvesting is a collection of；Whenever capsule is into brown
When, you can harvest；The seed shady place of harvesting is air-dried, and threshing is after preservation in low temperature, drying, closed environment.
The sterile line MSTZ-1 that the present invention is bred as has the property that：
Floral organ is characterized as：Sterile line MSTZ-1 is consistent with maintainer TZ-1 inflorescence properties, is compact inflorescence, pink
Corolla, gynoecium normal growth, as staminody, staminody type sterile line.
Varietal character is characterized as：Comprehensive resistance is stronger, high anti-weather spot disease resistance, resists the red disease of black shin and bacterial wilt；Cigarette
Leaf presentation quality is good, and color is orange, and colourity is strong, and oil content is good；Tobacco leaf chemical composition is coordinated, and judging parameter is good.
1 feature
MSTZ-1 is transplanted to buddingging 55 days, growing stage 128 days.Plant tubular, blade lanceolar, leaf color is green, blade face
Relatively put down, blade tip is tapering, and leaf margin microwave, mesophyll tissue is loose, and cauline leaf angle is medium, and blade master pulse thickness is medium, in axillary bud growing way
Deng.Plant height of pinching 130cm, effective blade number 23 or so, stem girth 9.53cm, pitch 5.22cm, the long 82.54cm of waist leaf, waist leaf
Wide 28.92cm, the long 64.72cm of top, the wide 17.75cm of top.Compared with K326, MSTZ-1 plant are higher, can picking leaves number quite,
Quite, waist leaf, top are longer for stem girth, pitch.
3 economic characters
MSTZ-1 per mu yield 142.11kg, 2468.68 yuan of average yield per mu value, 17.36 yuan/kg of average price, first-class cigarette ratio
Rate is 46.94%, and upper medium grade cigarette ratio is 58.98%.
4 qualities of tobacco
4.1 presentation quality
MSTZ-1 Maturity of Tobacco Leaf is based on maturation, and small part is still ripe, and colourity is based on " strong~medium ", small part colourity
Denseer, oil content is based on " having " quality scale, and small part is slightly owed, and identity is medium, and blade construction is based on loose, and small part is still dredged
Pine.
4.2 main chemical
The average nicotine content 2.79% of tobacco leaf, total nitrogen 1.95%, reduced sugar 28.22%, potassium 2.23%, chlorine in the middle part of MSTZ-1
0.18%, compare with compareing K326, MSTZ-1 nicotine contents are suitable, and content of reducing sugar is slightly biased low, and potassium content is lower slightly, chemical composition
Harmony is suitable.
4.3 aesthetic quality
MSTZ-1 B2F rating sample Type of aroma belongs to flat-flavor, and preferably, fragrance richness is good for fragrance texture, fragrant
Makings, perfume quantity performance are slightly better than K326, and miscellaneous tolerance is lighter, and excitant is micro- to be had, and pleasant impression is relatively comfortable, and flue gas concentration is larger, cigarette
The fine and soft degree of gas is preferable, and strength is larger, good in taste, MSTZ-1 B2F rating samples totality judging parameter is slightly better than control
K326；MSTZ-1 C3F rating sample Type of aroma belongs to flat-flavor, and preferably, perfume quantity is sufficient, flue gas concentration, strength for aroma quality
Moderate, miscellaneous gas is lighter, and excitant is micro- to be had, and pleasant impression is purer comfortable, and in good taste, flue gas is more fine and soft, and flammability is stronger, grey
It is good, MSTZ-1 C3F rating samples totality judging parameter is better than control K326.
Brief description of the drawings
Fig. 1 is sterile line MSTZ-1 seed selection flows of the present invention.
Embodiment
Embodiments of the invention：
The flue-cured tobacco cultivars MSG28 and TZ-1 that the present invention is used are planted from country of Dohanykutato Intezet of Qingzhou Tobacco Inst., China Tobacco Corp.
Matter resources bank is commercially available.
The selection of flue-cured tobacco sterile line new varieties, is comprised the following steps：
（1）Hybridization：Using sterile line MSG28 as female parent, using TZ-1 as male parent, hybridization obtains male sterility cross combination F1Kind
Son, F1Seed mixes sowing, and seed numbering is preserved；
（2）Continuous backcross：In next year, male sterility cross combination F is planted respectively1And TZ-1, with male sterility cross combination F1
For female parent, using TZ-1 as recurrent parent, backcrossing obtains BC1F1Seed, BC1F1Seed mixes sowing；In the follow-up time, with reference to sea
Nan Jiadai, using TZ-1 as recurrent parent, and backcross progeny generation of continuous backcross 5 again, until obtaining BC6F1Seed, it is substantially complete to reach
Into the purpose that MSG28 nucleus is replaced with TZ-1 nucleus, inheritance stability, completely sterile cytoplasmic male sterile line are cultivated into
MSTZ-1。
Wherein, " Hainan adds generation " refers to carrying out breeding material using the light and temperature condition not available for Hainan in Winter continent
Breeding plus generation, to accelerate breeding process.
Breeding male sterile lines new varieties MSTZ-1 method, is comprised the following steps：
（1）Plantation：Using MSTZ-1 as female parent, using TZ-1 as male parent, the sowing of the Parent same period, the same period transplant, male parent and mother
This is according to 1:Plant at 4 column number proportion intervals；
（2）Roguing:When cigarette strain is buddingged, hybrid strain is strictly gone, weak plant is gone, goes diseased plant, and thoroughly remove cigarette wooden fork, pressed
Type strain bagging is selected according to parent's standard；
（3）Artificial pollination：When MSTZ-1 corollas top occur it is red without it is open when, cut off with scissors corolla top or
Corolla is gently torn with finger, exposes gynoecium, then TZ-1 pollen is evenly coated on the column cap of sterile line gynoecium；
（4）Timely sowing：It is ripe a collection of according to the sequencing of hybridization pollination, harvest in time it is a collection of, whenever capsule is into brown
During color, maturation can be harvested seed, and the seed shady place of harvesting is air-dried, and threshing is after guarantor in low temperature, drying, closed environment
Deposit.
Claims (3)
1. a kind of selection of flue-cured tobacco sterile line new varieties, it is characterised in that：It is close by samsara of TZ-1 using MSG28 as female parent
This, sterile line new varieties MSTZ-1 is bred as by continuous backcross；Specifically comprise the following steps：
（1）Hybridization：Using MSG28 as female parent, using TZ-1 as male parent, hybridization obtains F1Seed；
（2）Continuous backcross：With F1For female parent, using TZ-1 as recurrent parent, backcrossing obtains BC1F1Seed；It is close by samsara of TZ-1
This, and backcross progeny generation of continuous backcross 5 again, until obtaining BC6F1Seed, as sterile line new varieties MSTZ-1.
2. breed the method for the flue-cured tobacco sterile line new varieties described in claim 1, it is characterised in that：Using MSTZ-1 as female parent, with
TZ-1 is male parent, breeds MSTZ-1.
3. the method for the flue-cured tobacco sterile line new varieties described in breeding claim 1 according to claim 2, its feature exists
In comprising the following steps：
（1）Plantation：Using MSTZ-1 as female parent, using TZ-1 as male parent, the sowing of the Parent same period, the same period are transplanted, and male parent is pressed with female parent
According to 1:Plant at 4 column number proportion intervals；
（2）Roguing:When cigarette strain is buddingged, hybrid strain is strictly gone, weak plant is gone, goes diseased plant, and thoroughly remove cigarette wooden fork, according to parent
This standard selects type strain bagging；
（3）Artificial pollination：When MSTZ-1 corollas top red occurs without opening, gynoecium is exposed using manual method, so
TZ-1 pollen is evenly coated on the column cap of gynoecium afterwards；
（4）Timely sowing：According to the sequencing of hybridization pollination, ripe a collection of, harvesting is a collection of；When capsule is into brown, i.e.,
It can harvest；The seed shady place of harvesting is air-dried, and threshing is after preservation in low temperature, drying, closed environment.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030115642A1 (en) * | 2001-09-21 | 2003-06-19 | Rufty Rebeca C. | Tobacco cultivar NC 2000 |
| CN103299895A (en) * | 2013-02-27 | 2013-09-18 | 中国农业科学院烟草研究所 | Rapid creation method of cytoplasmic male sterility (CMS) line |
| CN104885930A (en) * | 2015-07-01 | 2015-09-09 | 湖北省烟草科学研究院 | Breeding method for multi-resistance flue-cured tobacco hybrid |
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2015
- 2015-11-02 CN CN201510729907.6A patent/CN105210856B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030115642A1 (en) * | 2001-09-21 | 2003-06-19 | Rufty Rebeca C. | Tobacco cultivar NC 2000 |
| CN103299895A (en) * | 2013-02-27 | 2013-09-18 | 中国农业科学院烟草研究所 | Rapid creation method of cytoplasmic male sterility (CMS) line |
| CN104885930A (en) * | 2015-07-01 | 2015-09-09 | 湖北省烟草科学研究院 | Breeding method for multi-resistance flue-cured tobacco hybrid |
Non-Patent Citations (1)
| Title |
|---|
| 5个烤烟新品系的鉴定;王军等;《农技服务》;20091231;第26卷(第11期);第113-115页，尤其是摘要以及115页最后一段 * |
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