CN105199834A - Method for preparing wild rose seed oil by enzymolysis-cold pressing process - Google Patents
Method for preparing wild rose seed oil by enzymolysis-cold pressing process Download PDFInfo
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- CN105199834A CN105199834A CN201510657813.2A CN201510657813A CN105199834A CN 105199834 A CN105199834 A CN 105199834A CN 201510657813 A CN201510657813 A CN 201510657813A CN 105199834 A CN105199834 A CN 105199834A
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Abstract
The invention aims to provide an enzymolysis-cold pressing method for preparing rose seed oil. The method solves the problem of organic solvent residues in the solvent extraction process and the problem that a certain amount of trans fatty acids can be easily generated in the traditional hot pressing technique, and maximally maintains the nutrients. The method is characterized by comprising the following steps: sufficiently crushing plant cell walls by mechanical external force so that the plant cell walls sufficiently contact an enzyme, adding a composite enzyme, carrying out composite enzyme enzymolysis to maximally free oil, carrying out low-temperature drying to regulate the water content, and carrying out cold pressing. The oil yield is enhanced to 66.1%, and no trans fatty acids are generated in the whole extraction process.
Description
The present invention relates to fats and oils processing field, be specifically related to a kind of method that enzymolysis cold-press produces wild rose seed oil.
Background technology
Wild rose (Roseacicularis) is the Rosaceae, gul, and undershrub, also known as rose hip.Mainly be distributed in Xiaoxing ' anling Mountains of Heilongjiang Province areas to the south, be often born in opening or border, barren-resistant, drought-resistant, at the very low ground, sandy beach of organic content, riverbank, deserted mountain barren hill and road both sides well-grown.Rose hip fruit has VC content in anti-ageing, the effect, particularly pulp such as radioprotective, raising immunizing power and, up to (43 ~ 72) g/kg, is described as " king of dimension ", " flowers of life ".Rose hip is spent and can be extracted essence, and wild rose volatile oil is spices the most expensive in the market, has very strong persistence.Containing a large number of nutrients in wild rose rose-seed oil, be mainly linolenic and linoleic, linolenic acid content is 29.77%, linoleic acid content is 58.10%.
Oil extracting methods domestic at present concentrates on pressure sintering, solvent extration, and laboratory is commonly used Soxhlet extraction method and carried oil.The rose-seed oil that solvent extration extracts has organic solvent residual, and hot moulding process inevitably reduces the nutritive value of rose-seed oil, produces a certain amount of trans fatty acid, reduces the nutritive value of rose-seed oil.Cold-press can preserve the natural component in grease well, but directly cold press is not suitable for the seed of low oil content, and tooling cost is higher, be uneconomic for this non-high price product of rose-seed oil, the higher and time gradient that is that dissolve in supercutical fluid due to grease in rose-seed oil and fragrance matter of supercritical extraction cost is different easily causes being separated of grease and fragrance matter.The present invention adopts enzymolysis cold-press preparing rose seed oil, has both improve the oil yield of rose seed, and has protected again the natural nutrient component in rose-seed oil.
Summary of the invention
The present invention can produce the problems such as certain trans-isomer(ide) to solve to extract in rose-seed oil hot moulding process and improve oil yield, and enzymolysis cold-press preparing rose seed oil is a kind of method of novel extraction rose-seed oil.Extract rose-seed oil by the method for enzymolysis cold press from rose seed to be realized by following steps: step one, make plant cell wall broken by mechanical external force; Step 2, add prozyme to pulverize after rose seed carry out enzymolysis; Step 3, under the enzymatic hydrolysis condition of the best, cold press is carried out to the rose seed after enzymolysis.
Enzymolysis cold-press is utilized rose-seed oil to be extracted to the natural component not only can preserved well in grease, turn improve the oil yield of rose seed, final oil yield is 66.1%, oil yield gets a promotion compared with common extraction process, farthest saves the natural nutrient component in grease and produces without trans fatty acid.
Embodiment
Embodiment one: a kind of method that enzymolysis cold-press produces wild rose seed oil is realized by following steps: one, make plant cell wall broken by mechanical external force, two, add prozyme (plant hydrolyzed-enzyme: cellulase: compound protease is 1:3:1) and enzymolysis is carried out to the rose seed after pulverizing, because enzymatic reaction is by hydrolysis temperature, enzymolysis pH value, the impact of enzyme dosage and enzymolysis time, determine the processing condition producing wild rose seed oil with enzymolysis process: the rose seed after pulverizing is placed on 35, 40, 45, 50, in the thermostat water bath of 55 DEG C, enzymolysis pH is regulated to be 5.5, 6.0, 6.5, 7.0, 7.5, enzyme dosage scope is 1%, 2%, 3%, 4%, 5% (w/w), enzymolysis 1.5, 2.5, 3.5, 4.5, under 5.5h condition, carry out enzyme digestion reaction under these conditions, oven drying at low temperature carries out cold press after regulating moisture, take the quality of oil, obtain oil yield efficiency, three, under the enzymatic hydrolysis condition of the best, cold press is carried out to the rose seed after enzymolysis, in water content 7%, 8%, 9%, 10%, 11% (w/w), cold press time 7,8,9,10,11min, squeeze bore temperature 65,70,75,80,85 DEG C, squeeze gun pressure power 25,30,35,40, measure the oil yield of rose seed and the content of total trans fatty acid under the condition of 45MPa.
Embodiment two: the difference of present embodiment and embodiment one is to be placed in the thermostat water bath of 40,45,50 DEG C by the rose seed after pulverizing in step 2, enzymolysis pH is regulated to be 6.0,6.5,7.0, enzyme dosage scope is 2%, 3%, 4% (w/w), enzymolysis 2.5,3.5, carry out enzyme digestion reaction under 4.5h condition, oven drying at low temperature carries out cold press after regulating moisture, draws oil yield efficiency.Other step is identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is to carry out enzymolysis to rose seed in step 3 under the enzymatic hydrolysis condition of the best, then in water content 8%, 9%, 10% (w/w), cold press time 5,10,15min, squeeze bore temperature 70,75,80 DEG C, squeeze gun pressure power 30,35, under the condition of 40MPa cold press through the rose seed of enzymolysis, draw the content of oil yield and total trans fatty acid.Other step is identical with embodiment one.
Claims (3)
1. enzymolysis cold-press produces a method for wild rose seed oil, it is characterized in that extracting rose-seed oil by the method for enzymolysis cold press from rose seed is realized by following steps: one, make plant cell wall broken by mechanical external force, two, add prozyme (plant hydrolyzed-enzyme: cellulase: compound protease is 1:3:1) and enzymolysis is carried out to the rose seed after pulverizing, because enzymatic reaction is by hydrolysis temperature, enzymolysis pH value, the impact of enzyme dosage and enzymolysis time, determine the processing condition producing wild rose seed oil with enzymolysis process: the rose seed after pulverizing is placed on 35, 40, 45, 50, in the thermostat water bath of 55 DEG C, enzymolysis pH is regulated to be 5.5, 6.0, 6.5, 7.0, 7.5, enzyme dosage scope is 1%, 2%, 3%, 4%, 5% (w/w), enzymolysis 1.5, 2.5, 3.5, 4.5, under 5.5h condition, carry out enzyme digestion reaction under these conditions, oven drying at low temperature carries out cold press after regulating moisture, take the quality of oil, obtain oil yield efficiency, three, under the enzymatic hydrolysis condition of the best, cold press is carried out to the rose seed after enzymolysis, in water content 7%, 8%, 9%, 10%, 11% (w/w), cold press time 7,8,9,10,11min, squeeze bore temperature 65,70,75,80,85 DEG C, squeeze gun pressure power 25,30,35,40, measure total content of trans fatty acids of oil yield and rose-seed oil under the condition of 45MPa.
2. a kind of enzymolysis cold-press according to claim 1 produces the method for wild rose seed oil, it is characterized in that in step 2, the rose seed after pulverizing being placed in the thermostat water bath of 40,45,50 DEG C, enzymolysis pH is regulated to be 6.0,6.5,7.0, enzyme dosage scope is 2%, 3%, 4% (w/w), enzymolysis 2.5,3.5, carry out enzyme digestion reaction under 4.5h condition, oven drying at low temperature carries out cold press after regulating moisture, draws oil yield efficiency.
3. a kind of enzymolysis cold-press according to claim 1 produces the method for wild rose seed oil, it is characterized in that under the enzymatic hydrolysis condition of the best, carrying out enzymolysis to rose seed in step 3, then in water content 8%, 9%, 10% (w/w), cold press time 5,10,15min, squeeze bore temperature 70,75,80 DEG C, squeeze gun pressure power 30,35, under the condition of 40MPa cold press through the rose seed of enzymolysis, draw oil yield and the total content of trans fatty acids of rose-seed oil.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510657813.2A CN105199834A (en) | 2015-10-13 | 2015-10-13 | Method for preparing wild rose seed oil by enzymolysis-cold pressing process |
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| CN201510657813.2A CN105199834A (en) | 2015-10-13 | 2015-10-13 | Method for preparing wild rose seed oil by enzymolysis-cold pressing process |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105886100A (en) * | 2016-05-18 | 2016-08-24 | 蚌埠市九华油脂有限公司 | Preparation method of high-quality pomegranate seed oil |
| CN110042001A (en) * | 2019-05-05 | 2019-07-23 | 黑龙江省睿墨笛科技有限责任公司 | A kind of post-processing approach of wild rose fruit seed oil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103351944A (en) * | 2013-04-11 | 2013-10-16 | 石河子市天成油脂有限公司 | Method for extracting grape seed oil by cold pressing |
| CN104629890A (en) * | 2015-01-21 | 2015-05-20 | 东北农业大学 | Method for preparing pumpkin seed oil through enzymolysis cold squeezing method |
-
2015
- 2015-10-13 CN CN201510657813.2A patent/CN105199834A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103351944A (en) * | 2013-04-11 | 2013-10-16 | 石河子市天成油脂有限公司 | Method for extracting grape seed oil by cold pressing |
| CN104629890A (en) * | 2015-01-21 | 2015-05-20 | 东北农业大学 | Method for preparing pumpkin seed oil through enzymolysis cold squeezing method |
Non-Patent Citations (1)
| Title |
|---|
| CONCHA, C. SOTO, R. CHAMY, AND M.E. ZÚÑIGA: "Enzymatic Pretreatment on Rose-Hip Oil Extraction:Hydrolysis and Pressing Conditions", 《JAOCS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105886100A (en) * | 2016-05-18 | 2016-08-24 | 蚌埠市九华油脂有限公司 | Preparation method of high-quality pomegranate seed oil |
| CN110042001A (en) * | 2019-05-05 | 2019-07-23 | 黑龙江省睿墨笛科技有限责任公司 | A kind of post-processing approach of wild rose fruit seed oil |
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Application publication date: 20151230 |
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