CN103627514A - Method for extracting grape seed oil from grape seeds - Google Patents
Method for extracting grape seed oil from grape seeds Download PDFInfo
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- CN103627514A CN103627514A CN201210305028.7A CN201210305028A CN103627514A CN 103627514 A CN103627514 A CN 103627514A CN 201210305028 A CN201210305028 A CN 201210305028A CN 103627514 A CN103627514 A CN 103627514A
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- vitis viniferae
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Abstract
The invention discloses a method for extracting grape seed oil from grape seeds. The method comprises: successively performing crushing, enzymatic hydrolysis, organic solvent extraction, hot-cold alternate processing, active carbon decoloring and filtering on grape seeds for obtaining grape seed oil. According to the method, waste grape seeds are taken as the raw material for extraction, so that the waste is utilized, and resource waste is effectively avoided. By employing the method provided by the invention, the oil yield is high up to 95% or more, the anthocyanidin extraction rate is high up to 65%, the nutritional ingredients of the grape seed oil is furthest kept, also the harmful impurities in grape seed crude oil is eliminated, the quality of the grape seed oil is improved, and the prepared grape seed oil is naturally light yellow or light green.
Description
Technical field
The invention belongs to food processing technology field, be specifically related to extract the method for raisin seed oil from Semen Vitis viniferae.
Background technology
China's grape resource is abundant, the 5th, cultivated area and the output Jun Lie world, and 517.6 ten thousand tons of annual production, Semen Vitis viniferae is mainly derived from the tankage of Wine Enterprises, accounts for 5～7% of grape gross weight, if not being used, will cause the huge waste of resource.In Semen Vitis viniferae, contain large number of biological activeconstituents, containing pycnogenols approximately 8%, raisin seed oil approximately 12%.In raisin seed oil, contain palmitinic acid, oleic acid, linolic acid and micro-linolenic acid, lauric acid, Semen Myristicae etc., unsaturated fatty acid content reaches more than 90%, and linoleic acid content reaches 65～85%, is a kind of edible oil that has nourishing function.Pycnogenols (OPC) has removing free radical, the effect of inhibited oxidation damage.The Green Tea Extract oxidation capacity of OPC is 50 times of vitamin-E; ascorbic 20 times; and oral absorption is good; enter human body and can reach the highest haemoconcentration in 20 minutes; metabolic half life is for 7 hours; at aspects such as improving blood circulation, sight protectio, elimination oedema, skin care, memory, all having good effect, is rare beauty and health care good merchantable brand.
The method of extracting at present raisin seed oil from Semen Vitis viniferae comprises water extraction and alcohol precipitation method and supercritical extraction technique, adopts water extraction and alcohol precipitation method, and Semen Vitis viniferae loss of effective components is more, and extraction yield is low, and cost is also higher; And supercritical CO
2abstraction technique, method is complicated, complex operation, the problem such as output is little, and equipment investment is higher.
Summary of the invention
It is little that the technical problem to be solved in the present invention is to provide a kind of equipment investment, the method of extracting raisin seed oil from Semen Vitis viniferae that production cost is low, and the method oil yield is up to 95%, and anthocyanidin extraction yield is up to 65%, the raisin seed oil color and luster that extraction obtains is good, is naturally faint yellow or light green.
Technical scheme provided by the invention is from Semen Vitis viniferae, to extract the method for raisin seed oil, comprises the following steps:
1) Semen Vitis viniferae is ground into 10～40 order meal;
2) Semen Vitis viniferae meal is placed in to container, adds water and mix, the cellulase that adds 8～120,000 unit of activity/0.1g Semen Vitis viniferae powder carries out enzymolysis, obtains enzymolysis solution;
3) to the organic solvent lixiviate that adds 4～10 times of its weight in enzymolysis solution, extract 3 times at 60～70 ℃, each 4～6 hours, united extraction liquid, reclaimed solvent, obtains raisin seed oil;
4) by the hot deepfreeze of Semen Vitis viniferae crude oil 3 times, then be cooled to-1～-5 ℃ freezing 1～3 hour, filter, get filtrate activated carbon decolorizing, cooling, elimination impurity, obtains raisin seed oil;
Described hot deepfreeze is to be first warming up to 50～80 ℃ to process 1～3 hour, then is cooled to 0～8 ℃ of processing 1～6 hour, elimination precipitation;
Described decolouring is to decolour 30～60 minutes under 70～80 ℃, 0.6～0.8MPa, and activated carbon dosage is 1%～5% of Semen Vitis viniferae crude oil weight.
According to specific embodiment of the invention scheme, the method for extracting raisin seed oil from Semen Vitis viniferae of the present invention, step 2) in, Semen Vitis viniferae meal being added to water and mix, the add-on of water is 25%～50% of the young meal weight of grape.Water is tap water, is preferably deionized water.
According to specific embodiment of the invention scheme, the method of extracting raisin seed oil from Semen Vitis viniferae of the present invention, step 2) in, described enzymolysis is after Semen Vitis viniferae powder is mixed with water, with lemon acid for adjusting pH value to 4.6～5.0, under 40～50 ℃ of conditions, enzymolysis is 0.5～1 hour.After enzymolysis processing, Semen Vitis viniferae cellularstructure and grease compounded body destroy, and for extracting effective components, have created good condition, not only can improve oil yield, the extraction yield of pycnogenols be can also improve, protein and the further processing and utilization of other compositions in oil meal also can be made simultaneously.
According to specific embodiment of the invention scheme, the method for extracting raisin seed oil from Semen Vitis viniferae of the present invention, in step 3), described organic solvent is methyl alcohol, ethanol, acetone or butanols, its volumetric concentration is 60～95%.Described recovery solvent is that extracting solution reduction vaporization under 80～85 ℃, 0.02～0.04MPa condition is reclaimed to solvent.
According to specific embodiment of the invention scheme, the method for extracting raisin seed oil from Semen Vitis viniferae of the present invention, in step 4), the optimum condition of described decolouring is: bleaching temperature is 70 ℃, working pressure is 0.8MPa, bleaching time 30～50 minutes.
The present invention adopts the techniques such as hot deepfreeze, decolouring effectively to remove the various detrimental impurity such as water-soluble and fat-soluble in crude oil, has improved the quality of raisin seed oil, and the raisin seed oil color and luster of gained is good, is naturally faint yellow or light green.Technique of the present invention is simple, and facility investment is little, and energy consumption Di，Wei enterprise saves production cost, and adopts present method oil yield up to more than 95%, and pycnogenols extraction yield, up to more than 65%, has reduced the loss of the effective constituent in Semen Vitis viniferae to greatest extent.
Embodiment
The following examples contribute to the present invention of those skilled in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1
1) 10kg Semen Vitis viniferae is ground into 10 order meal;
2) Semen Vitis viniferae meal is placed in to container, adds 2.5kg water and mix, the cellulase that adds 80,000 unit of activity/0.1g Semen Vitis viniferae powder carries out enzymolysis, obtains enzymolysis solution;
3) in enzymolysis solution, add the lixiviate of 50L methyl alcohol, at 60 ℃, extract 3 times, each 4 hours, united extraction liquid, under 80 ℃, 0.02MPa condition, reduction vaporization reclaims solvent, obtains raisin seed oil;
4) by the hot deepfreeze of Semen Vitis viniferae crude oil 3 times, then be cooled to-1 ℃ freezing 1 hour, filter, get filtrate activated carbon decolorizing, cooling, elimination impurity, obtains raisin seed oil;
Described hot deepfreeze is to be first warming up to 50 ℃ to process 1 hour, then is cooled to 0 ℃ of processing 1 hour, elimination precipitation;
Described decolouring is to decolour 30 minutes under 70 ℃, 0.6MPa, and activated carbon dosage is 1% of Semen Vitis viniferae crude oil weight.
Oil yield is up to 95%, pycnogenols extraction rate reached 65%.
Embodiment 2
1) 10kg Semen Vitis viniferae is ground into 40 order meal;
2) Semen Vitis viniferae meal is placed in to container, adds 5kg water and mix, the cellulase that adds 120,000 unit of activity/0.1g Semen Vitis viniferae powder carries out enzymolysis, obtains enzymolysis solution;
3) in enzymolysis solution, add 150L alcohol steep, at 70 ℃, extract 3 times, each 6 hours, united extraction liquid, under 85 ℃, 0.04MPa condition, reduction vaporization reclaims solvent, obtains raisin seed oil;
4) by the hot deepfreeze of Semen Vitis viniferae crude oil 3 times, then be cooled to-5 ℃ freezing 3 hours, filter, get filtrate activated carbon decolorizing, cooling, elimination impurity, obtains raisin seed oil;
Described hot deepfreeze is to be first warming up to 80 ℃ to process 3 hours, then is cooled to 8 ℃ of processing 6 hours, elimination precipitation;
Described decolouring is to decolour 60 minutes under 80 ℃, 0.8MPa, and activated carbon dosage is 5% of Semen Vitis viniferae crude oil weight.
Oil yield is up to 96%, pycnogenols extraction rate reached 66%.
Embodiment 3
1) 10kg Semen Vitis viniferae is ground into 20 order meal;
2) Semen Vitis viniferae meal is placed in to container, adds 3kg water and mix, the cellulase that adds 100,000 unit of activity/0.1g Semen Vitis viniferae powder carries out enzymolysis, obtains enzymolysis solution;
3) in enzymolysis solution, add 78L acetone extraction, at 65 ℃, extract 3 times, each 5 hours, united extraction liquid, under 82 ℃, 0.03MPa condition, reduction vaporization reclaims solvent, obtains raisin seed oil;
4) by the hot deepfreeze of Semen Vitis viniferae crude oil 3 times, then be cooled to-3 ℃ freezing 3 hours, filter, get filtrate activated carbon decolorizing, cooling, elimination impurity, obtains raisin seed oil;
Described hot deepfreeze is to be first warming up to 60 ℃ to process 2 hours, then is cooled to 4 ℃ of processing 3 hours, elimination precipitation;
Described decolouring is to decolour 50 minutes under 70 ℃, 0.8MPa, and activated carbon dosage is 3% of Semen Vitis viniferae crude oil weight.
Oil yield is up to 98%, pycnogenols extraction rate reached 67%.
Embodiment 4
1) 10kg Semen Vitis viniferae is ground into 40 order meal;
2) Semen Vitis viniferae meal is placed in to container, adds 5kg water and mix, the cellulase that adds 80,000 unit of activity/0.1g Semen Vitis viniferae powder carries out enzymolysis, obtains enzymolysis solution;
3) in enzymolysis solution, add the lixiviate of 60L butanols, at 70 ℃, extract 3 times, each 4 hours, united extraction liquid, under 85 ℃, 0.02MPa condition, reduction vaporization reclaims solvent, obtains raisin seed oil;
4) by the hot deepfreeze of Semen Vitis viniferae crude oil 3 times, then be cooled to-5 ℃ freezing 1 hour, filter, get filtrate activated carbon decolorizing, cooling, elimination impurity, obtains raisin seed oil;
Described hot deepfreeze is to be first warming up to 50 ℃ to process 3 hours, then is cooled to 0 ℃ of processing 1 hour, elimination precipitation;
Described decolouring is to decolour 30 minutes under 70 ℃, 0.6MPa, and activated carbon dosage is 5% of Semen Vitis viniferae crude oil weight.
Oil yield is up to 95%, pycnogenols extraction rate reached 66%.
Claims (6)
1. from Semen Vitis viniferae, extract the method for raisin seed oil and pycnogenols, it is characterized in that: concrete steps are as follows:
1) Semen Vitis viniferae is ground into 10～40 order meal;
2) Semen Vitis viniferae meal is placed in to container, adds water and mix, the cellulase that adds 8～120,000 unit of activity/0.1g Semen Vitis viniferae powder carries out enzymolysis, obtains enzymolysis solution;
3) to the organic solvent lixiviate that adds 4～10 times of its weight in enzymolysis solution, extract 3 times at 60～70 ℃, each 4～6 hours, united extraction liquid, reclaimed solvent, obtains raisin seed oil;
4) by the hot deepfreeze of Semen Vitis viniferae crude oil 3 times, then be cooled to-1～-5 ℃ freezing 1～3 hour, filter, get filtrate activated carbon decolorizing, cooling, elimination impurity, obtains raisin seed oil;
Described hot deepfreeze is to be first warming up to 50～80 ℃ to process 1～3 hour, then is cooled to 0～8 ℃ of processing 1～6 hour, elimination precipitation;
Described decolouring is to decolour 30～60 minutes under 70～80 ℃, 0.6～0.8MPa, and activated carbon dosage is 1%～5% of Semen Vitis viniferae crude oil weight.
2. the method for extracting raisin seed oil from Semen Vitis viniferae according to claim 1, is characterized in that: step 2) in: the add-on of water is 25%～50% of Semen Vitis viniferae meal weight.
3. the method for extracting raisin seed oil from Semen Vitis viniferae according to claim 1, is characterized in that: step 2) in, described enzymolysis is by Semen Vitis viniferae mixed solution enzymolysis 0.5～1 hour under 40～50 ℃, pH value 4.6～5.0 conditions.
4. the method for extracting raisin seed oil from Semen Vitis viniferae according to claim 1, is characterized in that: in step 3), described recovery solvent is that extracting solution reduction vaporization under 80～85 ℃, 0.02～0.04MPa condition is reclaimed to solvent.
5. the method for extracting raisin seed oil from Semen Vitis viniferae according to claim 1, is characterized in that: in step 3), described organic solvent is methyl alcohol, ethanol, acetone or butanols, and its volumetric concentration is 60～95%.
6. the method for extracting raisin seed oil from Semen Vitis viniferae according to claim 1, is characterized in that: in step 4), described decolouring is to decolour 30～50 minutes under 70 ℃, 0.8MPa condition.
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| CN201210305028.7A CN103627514A (en) | 2012-08-24 | 2012-08-24 | Method for extracting grape seed oil from grape seeds |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357214A (en) * | 2014-11-13 | 2015-02-18 | 江苏金洲粮油食品有限公司 | Production method of grape seed oil |
| CN104845730A (en) * | 2015-04-30 | 2015-08-19 | 安庆市顺民粮油贸易有限公司 | Avocado oil extraction method |
| CN105111065A (en) * | 2015-08-05 | 2015-12-02 | 常州市鼎日环保科技有限公司 | Method for using grape seeds to prepare conjugated linoleic acid |
| CN105779115A (en) * | 2016-03-15 | 2016-07-20 | 安徽九禾农业发展有限公司 | Method for preparing wind-expelling cold-removing grape seed edible oil |
| CN106221904A (en) * | 2016-09-27 | 2016-12-14 | 乌鲁木齐上善元生物科技有限公司 | A kind of Oleum Vitis viniferae method of refining |
| CN106433948A (en) * | 2016-08-30 | 2017-02-22 | 闫涛 | Method for extracting grape seed oil through wetting-method squeezing |
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
| CN107307420A (en) * | 2017-07-04 | 2017-11-03 | 浙江绍兴东灵保健食品有限公司 | A kind of grape pip extract powder production technology |
| CN108835237A (en) * | 2018-06-05 | 2018-11-20 | 陈琪峰 | A kind of antistaling agent for grape |
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| JP2007056083A (en) * | 2005-08-23 | 2007-03-08 | Morinaga & Co Ltd | Method for producing deodorized vegetable oil |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357214A (en) * | 2014-11-13 | 2015-02-18 | 江苏金洲粮油食品有限公司 | Production method of grape seed oil |
| CN104845730A (en) * | 2015-04-30 | 2015-08-19 | 安庆市顺民粮油贸易有限公司 | Avocado oil extraction method |
| CN105111065A (en) * | 2015-08-05 | 2015-12-02 | 常州市鼎日环保科技有限公司 | Method for using grape seeds to prepare conjugated linoleic acid |
| CN105779115A (en) * | 2016-03-15 | 2016-07-20 | 安徽九禾农业发展有限公司 | Method for preparing wind-expelling cold-removing grape seed edible oil |
| CN106433948A (en) * | 2016-08-30 | 2017-02-22 | 闫涛 | Method for extracting grape seed oil through wetting-method squeezing |
| CN106221904A (en) * | 2016-09-27 | 2016-12-14 | 乌鲁木齐上善元生物科技有限公司 | A kind of Oleum Vitis viniferae method of refining |
| CN106929147A (en) * | 2017-03-10 | 2017-07-07 | 河南工业大学 | A kind of method for preparing oil body |
| CN107307420A (en) * | 2017-07-04 | 2017-11-03 | 浙江绍兴东灵保健食品有限公司 | A kind of grape pip extract powder production technology |
| CN108835237A (en) * | 2018-06-05 | 2018-11-20 | 陈琪峰 | A kind of antistaling agent for grape |
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Application publication date: 20140312 |