CN105137091B - Reagent storing device - Google Patents
Reagent storing device Download PDFInfo
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- CN105137091B CN105137091B CN201510397932.9A CN201510397932A CN105137091B CN 105137091 B CN105137091 B CN 105137091B CN 201510397932 A CN201510397932 A CN 201510397932A CN 105137091 B CN105137091 B CN 105137091B
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- reagent
- box
- accommodating chamber
- push rod
- reaction
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/726—Devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin
Abstract
The present invention relates to detection of biological samples technical field, more particularly to a kind of reagent storing device, the reagent storing device includes the reagent accommodating chamber for being used to deposit solid particle reagent or powder particle reagent, and the reagent accommodating chamber is sealed by seal.The present invention is individual components, it is encapsulated in reagent reacting box, solid particle reagent or powder particle reagent are sealed in reagent storing device, avoid random movement of the reagent in reagent reacting box, it is to avoid the problem of in the prior art needing that dried reagent is fixed, simultaneously, under sealing state, the term of validity storage life under the reagent normal temperature is also improved, it is distinguished with other reagents, the reagent for being difficult to ensure and depositing is compared under particularly suitable normal temperature.
Description
Technical field
The present invention relates to detection of biological samples technical field, more particularly to a kind of reagent storing device.
Background technology
Medical science in-vitro diagnosis plays the part of the role quite reused in medical industry now, the measurement that it can be qualitative or quantitative
The change of various Biological indicators in human fluid, to provide the consulting such as medical diagnosis on disease or treatment index, for example, the saccharification in blood
The detection of hemoglobin (HbA1c) is most important for the diagnosis and control of diabetes.
Glycosylated hemoglobin is the product that endoerythrocytic hemoglobin is combined with blood glucose in blood of human body, when Portugal in blood
When grape sugar concentration is higher, the HbA1c of human body formation content also can be of a relatively high.The average life span of red blood cell is 120 in human body
My god, before cell death, HbA1c content can also be remained relatively unchanged in blood, therefore, and glycosylated hemoglobin test is usual
Patient's glycemic control situation of nearly 8~12 weeks can be reflected, do not influenceed by a blood glucose rise or reduction once in a while.
Also there are a variety of concentration designed for detection and analysis thing in the prior art, such as：
As shown in figure 1, US1562237 discloses a kind of reaction box, reaction box includes reaction channel and liquid reagent is stored
Portion, reaction channel is provided with dry reagent, and liquid reagent storage part is used to deposit buffer solution or other liquid reagents, liquid examination
Agent storage part includes storage body 30', and storage body is sealed by seal or film 32', and film 32' has a distal end 33',
Directly tearing film from distal end makes film be separated with storage body, and the reagent stored in body is discharged into reaction channel.
When discharging liquid reagent, it is necessary to which the artificial film 32' that script is sealed on storage body 30' is separated, then
Film is removed.Although aforesaid way can tear film, discharge liquid reagent, by the distal end 33' of one section of extension from
Film is torn on storage body 30' to be extremely difficult, and overexertion or is firmly kicked the beam when such as artificially tearing and is easily caused film and break
Open or tear off not exclusively, prevent liquid reagent from discharging completely, liquid reagent is very few during detection, cause the inclined of testing result
Difference；On the other hand, in the non-detection period, film 32' is exposed aerial, artificial or other factorses easily occurs and rips or break
Bad risk.
As shown in Fig. 2 US5272093 is disclosed a kind of " reagent container and its delivering method ", including reagent storage chamber 12'
With the seal 40' being sealed in reagent storage chamber, seal is folding two layers setting, and one layer is used for sealed reagent and stores
Chamber, another layer extends to and extension 42' is formed outside reagent storage chamber, and extension 42' is used to tear in release reagent storage chamber
Reagent.When discharging liquid reagent, it is necessary to which the artificial seal 40' that script is sealed on reagent storage chamber 12' is separated,
It is easy to cause film to disconnect or tear off not exclusively if overexertion firmly kicks the beam, causes liquid reagent complete
Release, liquid reagent is very few when making detection, causes the deviation of testing result.
As shown in figure 3, US8846380 discloses a kind of " being used for the reaction box for detecting glycosylated hemoglobin concentration ", reaction
Box includes being used to accommodate the firstth area of kit blood sample, the secondth area, detection zone and reagent for accommodating wash solution
Reagent and wash solution in bag, reagent bag are stored separately, and are sealed up for safekeeping with aluminium foil 120'.When in reagent bag intercalation reaction box,
Aluminium foil is torn by reaction box, and the reagent and wash solution in reagent bag are temporarily stored in the first reaction zone of reaction box and second anti-respectively
Answer in area, reacted successively with blood sample by the rotation of reaction box, so as to solve storage and the assignment problem of reagent.
Above-mentioned reaction box, firstly, it is necessary to artificially fold the release portion 130' on aluminium foil, makes 130' pairs of release portion in detection
Clamper in quasi- detection box, then the reagent bag insertion after alignment is detected into box so that detection box can cut off release portion, realize
The separation of aluminium foil and reagent bag.This design is relative complex, and operating procedure is various, moreover, needing artificial alignment in grafting, easily
There is grafting situation not in place and cause grafting repeatedly.On the other hand, reaction box uses split-type design with reagent bag, reacts
The opened type design of box, foreign matter, if misoperation, is easily fallen into reaction box, impact the accuracy of testing result.Again
Person, in the non-detection period, release portion 130' is exposed aerial, the wind that artificial or other factorses are ripped or destroyed easily occurs
Danger.
The content of the invention
The technical problems to be solved by the invention are that there is provided a kind of reagent for the above-mentioned problems in the prior art
Memory.
To solve the above problems, the technical scheme is that：
A kind of reagent storing device, the reagent storing device includes being used to deposit solid particle reagent or powder particle reagent
Reagent accommodating chamber, the reagent accommodating chamber is sealed by seal.
Preferably, the solid particle reagent or powder particle reagent are lyophilized solid particle reagents or freeze-dried powder particle
Reagent.
Preferably, the solid particle reagent is that latex freezes ball.
Preferably, the reagent storing device also includes the reagent accommodating chamber for being used to deposit liquid reagent or powder reagent.
Preferably, it is described to be used to deposit to be provided with injection orifice in the reagent accommodating chamber of liquid reagent or powder reagent, inject
Hole connects reagent accommodating chamber and exterior space, and injection orifice is sealed by seal.
Preferably, the reagent storing device includes at least two reagent accommodating chambers, and at least two reagent accommodating chambers are into array
Distribution.
Preferably, the reagent storing device includes two reagent accommodating chambers, two reagent accommodating chambers or so it is transversely arranged or
Longitudinal arrangement up and down.
Preferably, the reagent storing device includes four reagent accommodating chambers, and four reagent accommodating chambers are in the column-shaped point of two row four
Cloth, often row is provided with two reagent accommodating chambers, and each column is provided with a reagent accommodating chamber.
Preferably, the back of the reagent storing device is provided with the cavity for being used for accommodating drier.
Prior art is compared to, the beneficial effects of the invention are as follows：
1. the reagent reacting box of the present invention is individually overall for one, reagent storage part and push rod are encapsulated in reagent reacting
Inside box, in reaction, it is only necessary to coordinate with detection box, reagent reacting box is inserted into external equipment by single stepping, you can
Realize the quick release of reagent in reagent storage part.Said structure is by the manually operated fractional reduction detected in reaction to most
Simplified degree, improves the automaticity of reaction, in reaction, and artificial manual operating procedure only includes sampling rod and gathered
Sample and by sample be added to reagent reacting box neutralization reagent reaction box insertion external equipment the step of.
2. the various pieces that the reagent reacting box of the present invention is included are housed in inside reagent reacting box, especially reagent
Storage part and push rod are included in inside reagent reacting box so that artificially can not touch reagent storage part and push rod manually, only
Putter action can just be promoted when being stretched into by the external thrust that box is detected in external equipment in reagent reacting box by having；Prevent not anti-
At once, because manual operation is improper or other factorses, seal part is torn off with reagent storage part, cause what reagent was spilt in advance
May.
3. it is that there is provided reagent reacting box for gap cooperation between the opening and each part on the reagent reacting box of the present invention
Internal simple reaction compartment, it is ensured that the purity of each reaction reagent, it is to avoid foreign be mixed into reagent reacting box can
Can, improve the precision of reaction.
4. the reagent storing device of the present invention is individual components, it is encapsulated in reagent reacting box, reagent storing device includes being used for
Deposit the reagent accommodating chamber of solid particle reagent or powder particle reagent.Solid particle reagent or powder particle reagent are sealed in examination
In agent memory, it is to avoid random movement of the reagent in reagent reacting box, it is to avoid need to enter dried reagent in the prior art
The problem of row is fixed, meanwhile, under sealing state, the term of validity storage life under the reagent normal temperature is also improved, makes itself and other examinations
Agent is distinguished, and the reagent for being difficult to ensure and depositing is compared under particularly suitable normal temperature.
5. the reagent storage part of the present invention freezes ball provided with latex, on the one hand realize and latex antibody reactivity is protected
Maximization, on the other hand, greatly improve term of validity storage life under reagent normal temperature.
6. the reagent reacting box of the present invention is by configuring the number of reagent accommodating chamber and its arrangement in reagent storage part, with pushing away
The cooperation of bar automation function, it is possible to achieve multiple reagents simultaneously discharge, multiple reagents according to the reaction time and order successively or
Add simultaneously and coordinate drying reagent, complete the possibility of various sample detections.
7. the detecting system of the present invention rapidly can promote push rod to act when reagent reacting box is inserted, meanwhile, it will try
What agent reaction box was quickly consolidated is fixed in detection box, it is ensured that detection zone is directed at light hole, i.e. single stepping, completes simultaneously
Reagent discharges, reagent reacting box is fixed and detection zone is to multiple functions such as light, most simplifies reactions steps.
Brief description of the drawings
Fig. 1 is the structural representation of reaction box in the prior art.
Fig. 2 is the structural representation of reagent container in the prior art.
Fig. 3 is the partial structural diagram of reaction box in the prior art.
Fig. 4 is the axonometric schematic diagram of partial structurtes in detecting system of the present invention.
Fig. 5 is the structural representation after panel is hidden in reagent reacting box of the present invention.
Fig. 6 is the structural representation after reagent reacting box dorsulum of the present invention is hidden.
Fig. 7 is the attachment structure schematic diagram of reagent storing device, seal and reagent release portion in the present invention.
Fig. 7 A are the structural representations after seal is torn in Fig. 7.
Fig. 8 is the structural representation of reagent storing device in the present invention.
Fig. 8 A are the structural representations of one angle of reagent storing device in the present invention.
Fig. 8 B are the structural representations of another angle of reagent storing device in the present invention.
Fig. 9 is a kind of structural representation of embodiment of reagent storing device in the present invention.
Figure 10 is the structural representation of reagent storing device another embodiment in the present invention.
Figure 11 is upper lid, reagent storing device and the attachment structure schematic diagram in reagent release portion in reagent reacting box of the present invention.
Figure 12 is the attachment structure schematic diagram of box body and push rod in reagent reacting box of the present invention.
Figure 12 A are the structural representations after push rod is slided with respect to box body in reagent reacting box of the present invention.
Figure 13 is the structural representation that box body is connected another embodiment with push rod in reagent reacting box of the present invention.
Figure 14 is the axle geodesic structure schematic diagram in release portion in reagent reacting box of the present invention.
Figure 15 is the mplifying structure schematic diagram at A in Fig. 6.
Figure 16 is the axle geodesic structure schematic diagram that box body is connected with push rod in reagent reacting box of the present invention.
Figure 16 A are the mplifying structure schematic diagrams at C in Figure 16.
Figure 17 is the axle geodesic structure signal of the another embodiment that box body is connected with push rod in reagent reacting box of the present invention
Figure.
Figure 18 is the mplifying structure schematic diagram at D in Fig. 5.
Figure 19 is the axonometric schematic diagram of reagent reacting box external structure of the present invention.
Figure 19 A are the backsight structural representations of reagent reacting box of the present invention.
Figure 20 is the structural representation of upper lid in reagent reacting box of the present invention.
Figure 20 A are the mplifying structure schematic diagrams at B in Figure 20.
Figure 20 B are another angled arrangement schematic diagrames of upper lid in reagent reacting box of the present invention.
Figure 21 is the attachment structure schematic diagram of upper lid and reagent storage part in reagent reacting box of the present invention.
Figure 22 is the explosive view of reagent reacting box of the present invention.
Figure 23 is the cross-sectional view of present invention detection box.
Figure 24 is the structural representation of internal part in present invention detection box.
Figure 25 is that reagent reacting box insertion of the present invention detects that push rod is by the cross-sectional view of push rod jack-up in box.
Figure 26 is the structural representation of elastic component in present invention detection box.
Figure 27 is the structural representation of shell fragment in present invention detection box.
Reagent view in step 0 when Figure 28 is reagent reacting box detection of the present invention.
Reagent view in step 1-6 when Figure 28 A-28F are reagent reacting box detection of the present invention respectively.
Embodiment
The present invention is further described with reference to the accompanying drawings and examples, but protection scope of the present invention is not limited to
This.
As shown in figure 4, the detecting system of the present invention is used for the concentration of analyte in quick detection biological sample, including examination
Agent reaction box 100 and external equipment, external equipment are the equipment in addition to the body of reagent reacting box 100, wherein, external equipment
Referred to as detection device, external equipment or detection device are provided with a detection box 200, and reagent reacting box 100 and detection box 200 are supporting
Use.Reagent reacting box 100 is used as the container of reaction, it is possible to achieve the storage of plurality of reagents, the order addition of plurality of reagents and
The functions such as devil liquor recovery.Detection box 200 is used to place reagent reacting box 100, and detection box 200 is arranged on detection device, and by
Detection device provide reagent reacting box 100 rotate, in reagent reacting box 100 reagent mix, the machine power of reagent release etc. and
Control, while being additionally provided with optics on external equipment, optics is used for the concentration for detecting detectable substance in fluid sample.
As shown in Figure 5 and Figure 6, reagent storage part 102, reagent release portion 150, reacting part are provided with reagent reacting box 100
With detection zone 28.Reagent storage part 102 and reagent release portion 150 are encapsulated in reagent reacting box 100.Preferably, the examination
A cavity is provided with agent reaction box 100, the reagent storage part 102 is housed in cavity.Reagent storage part 120 is individual components
It is encapsulated in reagent reacting box 100, is conducive to the quick assembling of reagent reacting box.Reagent storage part 102 is solid for sealed type storage
Body particle, powder or liquid reagent, reagent release portion 150 is detected in detection device can quickly open under the cooperation of box 200
Reagent storage part 102 discharges reagent into the reacting part of reagent reacting box 100.Reacting part is used for the temporary of biological sample and reagent
Deposit, mix and react, and detected by the intermediate product or final product of 28 pairs of reactions of detection zone.
As shown in Figure 7 and Figure 8, reagent storage part 102 includes at least one reagent accommodating chamber, and reagent storage part 102 is by close
Sealing 104 is sealed, i.e., reagent accommodating chamber is sealed by seal 104.Reagent storage part 102 is also referred to as reagent storing device, and reagent is deposited
Storage portion 102 includes multiple reagent accommodating chambers, and multiple reagent accommodating chambers are into array distribution.The quantity of reagent accommodating chamber is anti-according to detection
Reagent type that should be required, the order of addition are set, such as one, two or multiple, reagent accommodating chamber each other it
Between be independent, i.e., between multiple reagent accommodating chambers be provided with spacing, into array distribution.For example, two reagent accommodating chambers
It, into row pattern or longitudinal direction into column-shaped, can also be diagonal distribution that can be laterally；For another example, multiple reagent accommodating chambers can be with
It is spaced plug hole distribution.The capacity of reagent accommodating chamber is also that the dosage according to required for detection reaction is set.
As a preferred embodiment, the reagent storage part includes at least two row reagent accommodating chambers, each column is tried including at least one
Agent accommodating chamber.Preferably, as shown in figure 8, reagent storage part 102 includes two reagent accommodating chambers, two reagent accommodating chambers or so
It is transversely arranged.That is reagent accommodating chamber 106 and reagent accommodating chamber 108 is in the left and right distribution of a row two row, and the bottom of reagent accommodating chamber is big
Cause in the same horizontal line.Therefore, when tearing seal 104, the reagent in reagent accommodating chamber 106 and reagent accommodating chamber 108
Can together it be discharged into reagent reacting box 100.
As shown in figure 9, being used as a kind of embodiment of reagent storage part 102, the feelings of two reagent accommodating chamber longitudinal arrangements
Shape, i.e. reagent accommodating chamber 110 and reagent accommodating chamber 112 above and below two rows one row in being distributed, in partially torn seal 104, position
Reagent is preferentially discharged in the reagent accommodating chamber 112 of lower row, according to the requirement in reaction time, continues to tear seal 104, makes to be located at
The release reagent of reagent accommodating chamber 110 of upper row.Under the degree that control seal 104 is torn, it is possible to sequentially add reagent, control
The progress of system reaction.
As shown in Figure 10, as the another embodiment of reagent storage part, four reagent accommodating chambers are arranged in array
Situation, i.e. reagent accommodating chamber 114, reagent accommodating chamber 116, reagent accommodating chamber 118 and reagent accommodating chamber 120 are in the column-shaped point of two row four
Cloth.Under the degree that control seal 104 is torn, the reagent in the reagent accommodating chamber 118 and reagent accommodating chamber 120 of lower row is preferential
Release, the reagent then arranged in release.
As a preferred embodiment, the reagent storing device includes the examination for being used to deposit solid particle reagent or powder particle reagent
Agent accommodating chamber.Solid particle reagent or powder particle reagent be sealed in reagent storing device, it is to avoid reagent is in reagent reacting box
In random movement, it is to avoid the problem of in the prior art needing that dried reagent is fixed, meanwhile, under sealing state, also carry
Term of validity storage life under the high reagent normal temperature, makes it be distinguished with other reagents, compares under particularly suitable normal temperature and be difficult to ensure
The reagent deposited.
Preferably, the solid particle reagent or powder particle reagent are lyophilized solid particle reagents or freeze-dried powder particle
Reagent.Preferably, the solid particle reagent is that latex freezes ball.Latex freezes ball, on the one hand realizes anti-to latex antibody
The maximization of active protection is answered, on the other hand, term of validity storage life under reagent normal temperature is greatly improved.
As a preferred embodiment, the reagent storing device also includes the reagent accommodating chamber for being used to deposit liquid reagent.Reagent holds
Chamber of receiving can be used for storage solid particle, powder particle or liquid reagent, can be needed to be set according to detection.
As a preferred embodiment, as shown in Figure 8 A, at least one reagent accommodating chamber is provided with injection orifice 130, injection orifice 130 connects
Logical reagent accommodating chamber and exterior space, reagent accommodating chamber and injection orifice 130 are sealed by seal 104.Preferably, injection orifice
130 are located at the reagent accommodating chamber for being used for depositing powder reagent or liquid reagent.For eased sealing liquid reagent or
Powder reagent, it is first with the sealed reagent accommodating chamber of seal 104 and injection orifice 130 is exposed outside in sealing, then by liquid
Or powder reagent is injected into reagent accommodating chamber, then sealing injection hole 130 again, ensure that sealed effect, and reduce with this
The difficulty of sealing liquid or powder reagent.Reagent accommodating chamber and injection orifice can be sealed by same seal timesharing substep, can be by
Two seals are sealed respectively, such as using sealing plug sealing injection hole.
As a preferred embodiment, as shown in Figure 8 B, the chamber for installing drier is additionally provided with the back of reagent storage part 102
Body 132.When reagent storage part 102 is arranged in reagent reacting box 100, with cavity 132 and the inwall of panel 10, drier
In cavity 132.
As a preferred embodiment, reagent storage part 102 includes two reagent accommodating chambers, two reagent accommodating chambers are respectively used to deposit
Store up solid particle reagent and liquid reagent.The present embodiment is so that reagent storage part 102 includes two reagent accommodating chambers 106,108 as an example
It is described in detail, the storage solid particle reagent of reagent accommodating chamber 106 and the storage liquid reagent of reagent accommodating chamber 108, and in examination
The upper end of agent accommodating chamber 108 is provided with injection orifice 130.First solid particle reagent is placed in reagent accommodating chamber 106 in sealing,
Reagent is injected into the cavity hatch of the sealed reagent accommodating chamber 106 of seal 104 and reagent accommodating chamber 108, then by liquid reagent
In accommodating chamber 108, followed by the sealing injection hole 130 of seal 104.
As a preferred embodiment, the bottom design of reagent accommodating chamber takes the form of inclined plane.When discharging reagent, reagent is facilitated quickly to flow
Go out, residual of the reagent in accommodating chamber is reduced as far as possible, it is ensured that the precision of reagent volumes control.
As shown in fig. 7, reagent storage part 102 is sealed by seal 104, i.e., reagent is sealed in reagent accommodating chamber, it is close
Sealing 104 can be torn off in the presence of external force with reagent accommodating chamber, and tearing off for seal 104 can be by from left to right, under
Toward first-class mode, illustrated herein so that seal is torn off from lower to upper as an example, as shown in Figure 7 A.Seal 104 can be aluminium
The conventional use of seal in the prior art such as paper tinsel, film.
As is illustrated by figs. 11 and 12, reagent release portion 150 includes push rod 152, and push rod 152 is connected with seal 104, described
Push rod 152, which is used to cooperate with external equipment, makes seal be separated with reagent storage part.As shown in Figure 7 A, i.e. seal 104
Paste or be fixed on push rod 152, when external device acts on push rod 152, the inwall of push rod 152 and reagent reacting box 100
Coordinate the motion produced relative to reagent storage part 102, while push rod 152 is moved, the band relative reagent of dynamic seal 104 is deposited
Storage portion 102 is moved, i.e., the action that seal 104 is torn off so that the reagent in reagent accommodating chamber is discharged into reagent reacting box 100
In.The motion of the relative reagent storage part 102 of push rod 152 can be to be turned left by from left to right, by the right side, mode be realized from lower to upper etc..
As a preferred embodiment, as shown in fig. 7, one end sealed reagent storage part 102 of seal 104, after the other end is folded
It is pasted onto on push rod 152.
As a preferred embodiment, the push rod 152 is provided with the forced section cooperated with external equipment.Forced section is used to hold
The thrust of external equipment offer is provided.
As a preferred embodiment, push rod 152 is all housed in inside reagent reacting box 100, the thrust piece of external equipment needs
It extend into the inside of reagent reacting box 100 or acts on the forced section of push rod 152 by other means.Push rod 152 is all housed in
In reagent reacting box, human contact is artificially free-hand that push rod 152 can not be promoted to act less than the push rod in reagent reacting box, only
Have just can act push rod 152 after by external tool.So avoid in the non-detection period, maloperation or other reasons
Artificially tear or destroy the possibility that seal 104 causes reagent to leak in advance.
As a preferred embodiment, as shown in figure 13, chute 38 is provided with reagent reacting box 100.On the one hand, chute 38 is by push rod
152 are embedded in slideway, and on the other hand, chute 38 is slidably matched with push rod 152.As an embodiment, chute 38 is arranged on
On backboard 12, chute 38 can be designed as the only trough rim of backboard 12 and two composition, and now, 152 liang of lateral sections of push rod are housed in
In chute 38.
As a preferred embodiment, as shown in Figure 12 and Figure 12 A, the chute 38 is provided with division board 36, division board 36 is isolated
The inner space of chute 38 and reagent reacting box 100.As a kind of embodiment, chute 38 is the column of at least one end open
Body, four sides of column are made up of part backboard 12,38 liang of sides of chute and division board 36, that is to say, that chute 38 is close
Envelope connection division board 36, division board 36 separates the inner space of chute 38 and reagent reacting box 100, and the height of division board 36 is to react
It is design requirement that liquid, which will not be flowed to outside reagent reacting box,.Chute 38 is designed to an independent region, and push rod 152 or outside are pushed away
Power part is kept apart with reagent, can be avoided the contact with reaction reagent of push rod 152 or external thrust part and be taken away portion of reagent.
As a preferred embodiment, as shown in figure 14, at least one side of the push rod 152 is provided with least one limit convex
Play 154.Spacing preiection 154 can make the inside installed in reagent reacting box 100 that push rod 152 is more stablized.Preferably, it is spacing
The quantity of projection 154 is on two, the left and right side for being separately positioned on push rod 152.Preferably, the spacing preiection 154 is set
Top in the side of push rod 152.When push rod 152 is arranged in reagent reacting box 100, the spacing preiection 154 on push rod 152 exists
Under the extruding of reagent reacting box 100, push rod 152 is produced elastic deformation, add rubbing for push rod 152 and reagent reacting box 100
Wipe power, allow push rod 152 stablize be arranged on reagent reacting box 100 in, also increase push rod 152 move when resistance.
As a preferred embodiment, as shown in figure 14, the push rod 152 is provided with the cell body of at least one hollow out close to edge
156, the spacing preiection 154 is arranged on the lateral wall of cell body 156, and spacing preiection 154 is arranged in pairs with cell body 156.As
One specific embodiment, spacing preiection 154 is two, and two spacing preiections 154 are separately positioned on the left and right side of push rod 152
On, corresponding, the cell body 156 of hollow out is also two, is separately positioned on the left of push rod and right side is close to edge, i.e., left
The lateral wall of side cell body 156 is the left surface of push rod 152, and the lateral wall of right edge cell body 156 is the right flank of push rod 152,
That is, spacing preiection 154 is arranged on the lateral wall of cell body 156.The setting of hollow out cell body 156, when making the action of push rod 152
More be also easy to produce deformation, it is to avoid because rub it is excessive and caused by push rod 152 slide it is dumb.
If push rod 152 is chronically at deformed state and is easy for losing certain elasticity, and makes fastening effect not good, to solve
Above mentioned problem, as a preferred embodiment, as shown in figure 15, reagent reacting box 100 is provided with spacing recessed with the cooperation of spacing preiection 154
Groove 158.Preferably, chute 38 is provided with the limited impression 158 engaged with the spacing preiection 154, the quantity of limited impression 158
It is identical with spacing preiection 154.Push rod 152 is when under original state is normality, and limited impression 158 engages with spacing preiection 154, this
When, push rod 152 is not deformed upon；When promotion lower slider of the push rod 152 in external force, push rod 152 is produced at spacing preiection 154
Deformation simultaneously leaves limited impression 158.
As a preferred embodiment, as shown in figure 14, the push rod is provided with a groove 162.Groove 162 is arranged on push rod 152
Upper end, groove 162 for paste groove, paste groove be used for adhesive seal part 104.Before non-adhesive seal part, the bottom land of groove 162
Place plane is slightly below plane where the notch of groove 162, and after adhesive seal part 104, seal 104 fills and leads up groove 162, makes to push away
Bar plane is concordant.When avoiding the push rod 152 from promoting, because seal 104 is higher by the surface of push rod 152 and caused by the possibility peeled off.
As a preferred embodiment, the forced section of push rod 152 is the bottom surface or back of push rod, when the bottom that forced section is push rod
When, external force acts on the bottom surface of push rod, push rod 152 is torn seal 104 from lower to upper, when the back that forced section is push rod
When, external force acts on the back of push rod, equally promotes push rod 152 to tear seal 104 from lower to upper.The forced section of push rod 152
It can also be the upper bottom surface, left surface or right flank of push rod, when being upper bottom surface, push rod can be pulled from top；For left surface
When, push rod can be promoted from left to right；During for right flank, can turn left promotion push rod from the right side.
As another embodiment, the forced section of push rod 152 is connected with extraneous thrust piece magnetic, i.e., push rod 152 by
Power portion, extraneous thrust piece are the magnet assembly attracted each other, such as iron block, magnet.Push rod 152 and extraneous thrust piece are anti-by reagent
Answer box 100 to isolate, under magnetic action, drive push rod 152 to act by extraneous thrust piece.
As a preferred embodiment, reagent reacting box 100 is provided with an opening, forced section is exposed in said opening, forced section
External thrust is received by opening.I.e. external thrust part can be stretched into opening, connect with the push rod 152 in reagent reacting box 100
Touch and promote it to act, aperture position sets related to the forced section setting of push rod 152.
As a kind of embodiment, as shown in figure 16, the forced section of push rod 152 is the bottom surface of push rod, foregoing opening 34
It is arranged on the bottom plate 4 of reagent reacting box 100.Preferably, as shown in Figure 16 A, opening 34 is provided with chamfering 40, and push rod 152 is complete
Portion is housed in chute 38 and has certain interval apart from opening 34, to avoid collision during maloperation.External thrust part is by opening
Introduced at the chamfering 40 of mouth 34 and stretch into chute 38, contacted with push rod 152, and promote push rod 152 and external thrust part and chute
38 are slidably matched.
As another embodiment, as shown in figure 17, the forced section of push rod 152 is the back of push rod 152, foregoing opening
34 are arranged on the backboard 12 of reagent reacting box 100.Preferably, the back of push rod 152 is provided with fin or recess 160, convex
Rib or recess 160 are forced section, and fin or recess 160 are exposed in opening 34.External thrust part connects with fin or recess 160
Touch, and promote push rod 152 to be slidably matched with chute 38.
The reacting part includes at least one reaction zone, and reaction zone receives the reagent of the release of reagent storage part 102.Reacting part
Including multiple reaction zones, the quantity of reaction zone and position setting are according to the quantity of reagent accommodating chamber in reagent storage part 102 and instead
Answer step related, such as two kinds of reagents of release can be temporarily stored in a reaction zone simultaneously, can also be temporarily stored in respectively two each
In independent reaction zone；If there is drying reagent in reaction zone, other reaction zones can be also separately provided, phase between each reaction zone
It is intercommunicated, the mixing of reagent in each reaction zone can be realized by rotating reagent reacting box.
As shown in Figure 5 and Figure 6, detection zone 28 is provided with reacting part, detection zone 28 can be arranged on reagent reacting box 100
Any agent circulation passage on, detection zone 28 typically uses transparent material, makes transmitted light or scattering that optics is sent
Light enters reagent reacting box 100.Conducting element is provided between reagent storage part 102 and reacting part, more specifically, conducting element is set
Put between reagent accommodating chamber and corresponding reaction zone, conducting element makes the reagent in reagent accommodating chamber fast and accurately be discharged into instead
Answer in area.
As a preferred embodiment, as shown in Fig. 5 and Figure 18, reacting part includes at least one reaction zone, wherein at least one is anti-
It is the first reaction zone 26 to answer area, and first reaction zone 26 is used to keep in solid particle reagent, and the first reaction zone 26 includes support
Portion and stop part, form a gap 47 between supporting part and stop part, the Breadth Maximum in gap 47 is less than solid particle reagent
Minimum widith.It is other anti-that gap between supporting part and stop part is used to stop that the solid particle reagent in the first reaction zone to enter
Answer area, allow solid particle reagent stablize be temporarily stored on supporting part.As a kind of embodiment, supporting part is a step
46, stop part is a baffle plate, is designated as first baffle 48.First baffle 48 is vertical baffle plate, the bottom of first baffle 48 and branch
Support part forms gap 47.Preferably, the solid particle reagent is that latex freezes ball agent.The minimum that latex freezes ball agent is wide
Degree is Breadth Maximum of the diameter more than gap 47 that latex freezes ball, latex is freezed ball agent and is stopped by gap 47.
As a preferred embodiment, as shown in Fig. 5 and Figure 18, the first reaction zone 26 also includes second baffle 50, second baffle 50
It is obliquely installed, second baffle 50 is tried with the second gap 49 of stop part formation, the minimum widith in the second gap 49 more than solid particle
The Breadth Maximum of agent.Second baffle 50 is used for solid particle reagent drainage with stop part, enables solid particle reagent smoothly
Enter in the first reaction zone 26.As an embodiment, the Breadth Maximum that latex freezes ball agent is that latex is freezed
The diameter of ball is less than the minimum widith in the second gap 49, and freezing latex, ball agent is convenient to be entered in the first reaction zone 26.
As a preferred embodiment, first baffle 48 and second baffle 50 are the baffle plate with radian.On the one hand solid particle is made
Reagent can be rapidly entered, it is contemplated that the difference on Solids Particle Diameter, it is to avoid solid particle is stuck in first baffle 48 and second
It can not be fallen on step 46 between baffle plate 50.
As a preferred embodiment, the reacting part also includes second reaction zone, second reaction zone is used to keep in liquid reagent.It is excellent
Selection of land, second reaction zone is provided with conducting element, and conducting element includes the first drainage plate and the second drainage plate.
As a kind of embodiment, as shown in Figure 5 and Figure 6, reacting part includes the first reaction zone 26 and the second reaction
Area 25, the first reaction zone 26 is used for the solid particle for receiving the release of reagent accommodating chamber 106, and second reaction zone 25 is used to receive reagent
The liquid reagent that accommodating chamber 108 discharges.Second reaction zone 25 is provided with conducting element, and conducting element includes the first drainage plate 42 and second
Drainage plate 44, makes liquid reagent flow rapidly into second reaction zone 25.First reaction zone 26 includes the step for being used to keep in reagent
46th, first baffle 48 and second baffle 50, first baffle 48 and second baffle 50 are used to guide solid particle to enter step 46, together
When, gap 47 is formed between first baffle 48 and step 46, the gap 47 can prevent solid particle from entering second reaction zone 25,
And allow liquid reagent to flow into the first reaction zone 26.
As shown in Figure 19 and Figure 19 A, reagent reacting box 100 includes top plate 2, bottom plate 4, left plate 6, right plate 8, panel 10
With backboard 12.The substantially square box-like body of reagent reacting box 100, is made up of plastic material,
As a preferred embodiment, as shown in figure 19, the left plate 6 of the reagent reacting box 100 or one end of right plate 8 are one
Inclined-plane 22.Preferably, the backboard 12 of reagent reacting box 100 is provided with an extension 24, and extension 24 extends out to inclined-plane 22
It is outside.Preferably, inclined-plane 22 is arranged on the lower left corner of reagent reacting box 100.In one embodiment, backboard 12 is square,
Panel 10 be pentagon, left plate 6 have one side and an inclined-plane, the rectangular triangle of extension 24, right angled triangle
Hypotenuse is the junction of backboard 12 and extension 24.The setting on inclined-plane 22 and extension 24, is easy to artificially distinguish reagent reacting box
100 positive and negative, it is to avoid reagent reacting box 100 is reversely inserted into detection box 200, on the other hand, in reagent reacting box and inspection
, can be with the positive and negative of automatic identification reagent reacting box, it is to avoid maloperation when surveying box cooperation.
As a preferred embodiment, as shown in figure 20, reagent reacting box 100 is provided with positioning convex 16.Positioning convex 16 is set
On the faceplate 10, positioning convex 16 is in upside-down triangle shape, and such as Figure 20 A, positioning convex 16 is used for reagent reacting box 100 is firmly fixed
Position in the corresponding groove of detection box 200, positioning convex 16 with groove is spacing engages, reagent reacting box 100 is fastened on inspection
Survey in box 200, it is to avoid during rotation, the displacement of the relative detection box 200 of reagent reacting box 100.
As a preferred embodiment, as shown in Figure 19 A, the top plate 2 of reagent reacting box 100 is provided with handle 14, and in detection, side
Just user holds quick insert reagent reacting box 100 of handle 14 and detected in box 200, upon completion of the assays, and quick by reagent
Reaction box 100 is extracted.As a preferred embodiment, the backboard 12 of reagent reacting box 100 is provided with notch 52, the setting of notch 52 makes examination
The injection molding of agent reaction box 100 is more convenient.
As a kind of embodiment, as shown in Fig. 5, Fig. 6 and Figure 22, provided with reagent storage in reagent reacting box 100
Portion 102, reagent release portion 150, reacting part, detection zone 28, sampling rod 30 and liquid absorption pad 32, reagent reacting box 100, reagent
Storage part 102, reagent release portion 104 and sampling rod 30 are made by plastic material.Sampling rod 30 is used to gather fluid sample such as
Blood, urine etc., and fluid sample is added in reagent reacting box 100.Upon completion of the assays, liquid absorption pad 32 is used for back
Receive waste liquid, it is to avoid liquid leakage is polluted.
As shown in Fig. 5, Fig. 6 and Figure 22, reagent reacting box 100 is a cavity, can be divided into box body 18 and upper lid 20, box body 18
Including backboard 12, bottom plate 4, left plate 6 and right plate 8, upper lid 20 includes top plate 2 and panel 10, and upper lid 20 passes through with box body 18
The modes such as welding are tightly connected, and facilitate the assembling of reagent reacting box 100.The liquid absorption pad 32 is arranged on the upper of inclined-plane 22
Portion, liquid absorption pad 32 turn right successively side plate 8 direction be sampling rod 30 and reagent storage part 102, reacting part and reagent release
Portion 104 is arranged at the lower section of reagent storage part 102, and detection zone 28 is arranged in reacting part.Sampling rod 30 is set by an opening
Put in reagent reacting box 100, sampling rod 30 and the opened gap coordinate, it is to avoid during sampling, foreign matter is mixed into.Inclined-plane 22 is set
In the left side of sampling rod 30, the sample on sampling rod 30 is conducive to fully to be contacted with reaction solution, it is to avoid reaction solution is very few and contacts not
To sample.Liquid absorption pad 32, reagent storage part 102, sampling rod 30, reacting part and the general layout in reagent release portion 104 arrangement are not
It is limited to upper type.Preferably, the second drainage plate 44 is connected with the upper end of first baffle 48, and the upper end of the first drainage plate 42 is also associated with
Sampling needle guide plate 57, the vertical direction of sampling needle guide plate 57 is set.As shown in Figure 12 and Figure 20 B, box body 18 and upper lid 20 are equal
Provided with sampling needle fixture 59, for sampling needle fixture 59, it is to avoid it is rocked.
As a kind of embodiment, reagent storage part and reagent reacting box connected mode, as shown in Figure 8 A, reagent is deposited
Storage portion 102 is provided with keeper, and each keeper constitutes cavity, and the cavity is used to house reagent storage part 102 so that reagent is deposited
Storage portion 102 is fixedly mounted in reagent reacting box 100.The cavity can also use conventional technical means of the prior art.
As a specific embodiment, as shown in Figure 12 and 12A, Figure 20 and Figure 20 B, panel 10 is provided with locating dowel 54 and supporting plate
61, left positioner plate 56 and right positioner plate 58 are multiple scattered keepers, scattering device on box body 18 and upper lid 20, on
Stating keeper is used to be fixedly connected with reagent storage part 102.As shown in Figure 8 A and 8 B, the keeper of reagent storage part 102 includes
Mounting hole 122, left keeper 124, right keeper 126 and lower keeper 128.As shown in figure 21, mounting hole 122 is buckled in locating dowel
On 54, the upper end for fixating reagent storage part 102；Left keeper 124 and right keeper 126 respectively with left positioner plate 56 and
The spacing connection of right positioner plate 58, lower keeper 128 is placed in supporting plate 61.Therefore, the firm installation of reagent storage part 102
In reagent reacting box 100, it is to avoid displacement of the reagent storage part 102 during reagent reacting box 100 is moved or rocked.
Each above-mentioned can carry out any apolegamy and combination as needed preferably between embodiment, final to realize
The purpose of quick sample Concentration Testing.
As shown in figure 23 and figure 24, a push rod 204 is provided with detection box 200, push rod 204 passes through the institute on reagent reacting box
State the active force that opening 34 contacted and provided external equipment with forced section.
As a preferred embodiment, the push rod 204 relative can detect that box 200 is moved.Push rod 204 can be fixed positioned on box body
On, can also be with box body relative motion.During the activity of push rod 204, by the zone of action of peripheral equipment control push rod 204 and movement
Position, for example, can use the movement of motor control push rod 204, can also use routine techniques of the prior art.If push rod 204 is consolidated
It is scheduled on box body, when reagent reacting box 100 inserts the detection box 200 of external equipment, by insertion force, by push rod 204 and push rod
152 coordinate, and act push rod 152.
As a preferred embodiment, the push rod 204 is arranged on the bottom plate of detection box 200 or the push rod 204 is arranged on inspection
In the inner panel for surveying box 200.As shown in figure 25, when detection box 200 and forced section is the bottom surface of push rod 152, opening 34
It is arranged on the bottom plate 4 of reagent reacting box 100, push rod 204 is arranged on the bottom plate of detection box 200.Preferably, push rod 204
Upper end is provided with chamfering, facilitates push rod 204 to stretch into opening 34.When detecting that box 200 coordinates with forced section for the back of push rod 152,
Opening 34 is then arranged on the backboard 12 of reagent reacting box 100, and push rod 204 is arranged in the inner panel of detection box 200.
As a preferred embodiment, as shown in Figure 24 and Figure 26, a portable plate is provided with detection box 200, portable plate includes substrate
206 and elastic component 210, substrate 206 is fixedly connected on by elastic component 210 on the inwall of detection box 200.Preferably, elastic component
210 quantity is two, and elastic component 210 includes mounting surface 212 and two elastic arms 214, and mounting surface 212 is interior with detection box 200
Side is fixedly connected, and two elastic arms 214 are fixedly connected with substrate 206 respectively.
As a preferred embodiment, the substrate 206 is heating plate, the first heating plate is designated as.I.e. portable plate is used as heating member
It is used as elastic fastener again simultaneously.
As a preferred embodiment, one of medial surface of the detection box 200 is provided with shell fragment 216 as shown in figures 23-27.
Preferably, shell fragment 216 is arranged on one of medial surface adjacent with portable plate.Shell fragment 216 is provided with an elastic arm 218, elastic arm
218 one end are fixed with shell fragment 216, and the other end of elastic arm 218 is in smooth curved surface shape, and elastic arm 218 is towards the detection internal cavities of box 200
Install.Shell fragment 216 is close to the side for being not provided with inclined-plane on recoil case 100 to the inclined-plane 22 on reagents reaction box 100
The madial wall of detection box 200.Because shell fragment 216 has certain elasticity, if reagent reacting box 100 is reversely inserted into detection
When in box 200, due to the effect of shell fragment 216, reagent reacting box 100 can not be plugged together in detection box 200 completely, therefore, shell fragment
216 and inclined-plane 22 cooperation again can play the recognition reaction that reagent reacting box 100 is correctly inserted into.
As a preferred embodiment, as shown in figure 23, one of medial surface of the detection box 200 is provided with groove 202.It is excellent
Selection of land, groove 202 is arranged on the medial surface of relative portable plate.The convex that groove 202 is used on clamping reagent reacting box 100
16 are played, reagent reacting box 100 is connected on groove 202, it is to avoid detection box 200 is in the rotation or vibration processes, examination
The displacement of agent reaction box 100.
As a kind of embodiment, as shown in Fig. 4, Figure 23 and Figure 24, detection box 200 is fixed on external equipment,
Mix or rotate to realize, detection box 200 includes the box body of open at one end, box body is provided with light hole 201, box body provided with top
Bar 204, the first heating plate 206 and the second heating plate 208, the first heating plate 206 are portable plate, and push rod 204 passes through above-mentioned opening
34 contact with forced section and provide external thrust, and the first heating plate 206 and the second heating plate 208 are used to heat reagent, to meet
The requirement of reaction temperature.
As shown in figure 25, when reagent reacting box 100 is inserted into detection box 200, the pressing of reagent reacting box 100 first adds
Hot plate 206, the first heating plate 206 under pressure, deforms upon elastic component 210, and reagent reacting box 100 can be inserted quickly
Enter and fasten to positioning convex 16 in groove 202.Meanwhile, the compression of inclined-plane 22 shell fragment 216 of reagent reacting box 100 makes reagent
The light hole 201 on the alignment detection box 200 of detection zone 28 on reaction box 100.Detection box is inserted into reagent reacting box 100
During 200, push rod 204 stretches into opening and 34 contacted with push rod 152, with reagent reacting box 100 from top to bottom during,
Push rod 152 is acted from lower to upper, and band dynamic seal 104 is acted from lower to upper, seal 104 is torn off with reagent storage part 102,
Discharge reagent as shown in Figure 7 A.
Glycosylated hemoglobin detection method is many on existing market face, wherein conventional detection method has ion exchange layer
Analysis, affinity chromatography, high pressure liquid phase, immunization, Ion capture, electrophoresis etc..Immunization refers to after erythrocytolysis, is based on
The interactions of antigen molecule and distinct antibodies measures HbA1c.
Include following two detecting steps with immune agglutination method detection HbA1c：The total hemoglobin in sample is detected respectively
Hb concentration and Glycohemoglobin HbA1c concentration.The detection of total hemoglobin (Hb) aoxidizes blood red egg by using the potassium ferricyanide
Ferrous ion in white, generates ferrihemoglobin, and ferrihemoglobin and rhodanide reaction generation thiocyanic acid high ferro are blood red
Albumen, detects the light absorption value under 531nm, obtains Hb concentration.Glycosylated hemoglobin (HbA1c) containing multiple HbA1c with exempting from
HbA1c competition bindings in the agglutinin and blood in epidemic disease reaction bonded site are marked at the anti-HbA1c antibody on latex beads, preceding
The combination of person can cause the change of reaction solution turbidity, by detecting that 531nm extinction value changes can obtain HbA1c in blood
Concentration.HbA1c concentration is also high in blood, and turbidity is lower, and light absorption value is smaller, and light absorption value and the change of HbA1c concentration pass through school
Directrix curve is obtained.
Therefore, immunization detection HbA1c need to use rhodanate liquid reagent (Buffer), marked anti-HbA1c resist
Latex balloon, potassium ferricyanide dried reagent (drying object) and the agglutinin containing multiple HbA1c immune responses binding sites of body are done
Dry reagent (drying object).As shown in figure 28, rhodanate liquid reagent 60 is stored in reagent accommodating chamber 108, marked anti-HbA1c
The latex balloon 62 of antibody is stored in reagent accommodating chamber 106, and potassium ferricyanide dried reagent 64 is solidificated on second reaction zone 25, is coagulated
The plain dried reagent 66 of collection solidifies in the first reaction zone 26.
Wherein, latex balloon is that latex freezes bead, will connect upper spy by covalently bound method in latex bead in advance
Different in nature HbA1c antibody, then use freeze drying technology that latex balloon is quick-frozen for volume identical bead, realize to latex antibody response
The maximization of active protection, meanwhile, greatly improve term of validity storage life under normal temperature.
Utilize the concentration of HbA1C in the reagent reacting box detection blood of the present invention.The step of immunization detects HbA1c is such as
Under：
Step 0：As shown in figure 28, the sampling rod 30 with sample is inserted into reagent reacting box.
Step 1：As shown in Figure 28 A, reagent reacting box is inserted into detection box, push rod 152 is in outside by rotation detection box
Moved upwards in the presence of thrust, tear off seal 104, latex balloon 62 and rhodanate 60 are discharged simultaneously, and latex balloon 62 is fallen
Kept in into the first reaction zone 26, rhodanate liquid reagent 60 is fallen through into second reaction zone 25 in detection zone 28.
Step 2：As shown in Figure 28 B, reagent reacting box is rotated, by Buffer60 (rhodanate), (the iron cyaniding of drying object 64
Potassium) and blood sample mixing, form mixture X.
Step 3：As shown in fig. 28 c, reagent reacting box is rotated, the mixture X in step 2 is rotated to detection zone 28, inspection
The content of hemoglobin (Hb) in test sample product.
Step 4：As shown in fig. 28d, continue to rotate reagent reacting box, the mixture X in step 2 is entered the first reaction zone
26, mixture (marked anti-with drying object 66 (agglutinin containing multiple HbA1c immune responses binding sites) and latex balloon 62
The latex beads of HbA1c antibody) mixing, form mixture Y.
Step 5：As shown in Figure 28 E, reagent reacting box is rotated, the mixture Y in step 4 is entered detection zone 28, detection
The content of glycosylated hemoglobin (HbA1C) in sample.
Step 6：As shown in Figure 28 F, reagent reacting box is rotated, the mixture Y in step 4 is entered liquid absorption pad 32,
Absorbed reacted waste liquid.
HbA1c detections terminate, and are calculated detection structure by external equipment and are exported.
The reagent reacting box of the present invention is not limited to the detection of HbA1c in above-mentioned blood sample, can be used for other biological
The detection of sample such as urine, saliva, spinal fluid etc., it can also be used to C reactive protein, cholesterol, blood fat and blood glucose etc. in biological specimen
The Concentration Testing of analyte.
It is all not add special instruction in described above, using conventional technical means of the prior art.
Claims (9)
1. a kind of reaction box, including reagent storing device and reacting part, it is characterised in that the reagent storing device includes being used to deposit
The reagent accommodating chamber of solid particle reagent and the reagent accommodating chamber for depositing liquid reagent, it is described to be used to deposit solid particle examination
The reagent accommodating chamber of agent and sealed for the reagent accommodating chamber for depositing liquid reagent by seal；The reacting part includes at least the
One reaction zone and second reaction zone, first reaction zone are used to keep in solid particle reagent, in seal and reagent accommodating chamber
After separation, the solid particle reagent in reagent accommodating chamber is discharged into the first reaction zone and kept in, and the second reaction zone is received simultaneously
The liquid reagent of temporary reagent accommodating chamber release；
First reaction zone includes the step, first baffle and second baffle for being used to keep in solid particle reagent, described first
Baffle plate and second baffle are used to guide solid particle to enter step, meanwhile, the step and one gap of first baffle formation, the seam
The Breadth Maximum of gap is less than the minimum widith of solid particle reagent, and the gap can prevent solid particle from entering second reaction zone,
And allowing liquid reagent to flow into the first reaction zone, the second baffle is obliquely installed, second baffle and first baffle formation the
Two gaps, the minimum widith in the second gap is more than the Breadth Maximum of solid particle reagent.
2. reaction box according to claim 1, it is characterised in that the solid particle reagent tries for lyophilized solid particle
Agent.
3. reaction box according to claim 1, it is characterised in that the solid particle reagent is that latex freezes ball.
4. reaction box according to claim 1, it is characterised in that the reagent accommodating chamber for being used to deposit liquid reagent
Injection orifice, injection orifice connection reagent accommodating chamber and exterior space are provided with, injection orifice is sealed by seal.
5. reaction box according to claim 1, it is characterised in that the reagent for being used to deposit solid particle reagent is accommodated
Chamber and for deposit liquid reagent reagent accommodating chamber be in array distribution.
6. reaction box according to claim 5, it is characterised in that the reagent for being used to deposit solid particle reagent is accommodated
Chamber and reagent accommodating chamber for depositing liquid reagent or so are transversely arranged.
7. reaction box according to claim 1, it is characterised in that the back of the reagent storing device, which is provided with, to be used to accommodate
The cavity of drier.
8. reaction box according to claim 1, it is characterised in that reagent release portion, detection are additionally provided with the reaction box
Area, sampling rod and liquid absorption pad, the reagent release portion include push rod, and push rod is connected with seal, the push rod for
External equipment, which cooperates, makes seal be separated with reagent storage part.
9. reaction box according to claim 8, it is characterised in that the liquid reagent is rhodanate liquid reagent, Gu
Body particle reagents are to be solidified with drying object on the latex balloon that marked anti-HbA1c antibody, first reaction zone：Agglutinin is dried
Drying object is solidified with reagent, second reaction zone：Potassium ferricyanide dried reagent；
The reaction box is used for the concentration of HbA1C in immune detection blood, comprises the following steps：
Step 0：Sampling rod with sample is inserted into reagent reacting box；
Step 1：The external equipment includes detection box, and reaction box and detection box are supported the use；Rotation detection box, by reagent reacting
Box is inserted into detection box, and push rod is moved upwards in the presence of external thrust, tears off seal, and latex balloon and rhodanate are same
When discharge, latex balloon fall through into the first reaction zone keep in, rhodanate liquid reagent fall through into second reaction zone examine
Survey in area；
Step 2：Reagent reacting box is rotated, rhodanate Buffer, potassium ferricyanide drying object and blood sample are mixed, forms mixed
Compound X；
Step 3：Reagent reacting box is rotated, the mixture X in step 2 is rotated to detection zone, hemoglobin in sample is detected
(Hb) content；
Step 4：Continue to rotate reagent reacting box, the mixture X in step 2 is entered the first reaction zone, mixture and drying object
With latex balloon mixing, the drying object formed in mixture Y, step 4 is the aggegation containing multiple HbA1c immune responses binding sites
Latex balloon in element, step 4 is the latex beads that marked anti-HbA1C antibody；
Step 5：Reagent reacting box is rotated, the mixture Y in step 4 is entered glycosylated hemoglobin in detection zone, detection sample
(HbA1C) content；
Step 6：Reagent reacting box is rotated, the mixture Y in step 4 is entered liquid absorption pad, is inhaled reacted waste liquid
Receive；
HbA1c detections terminate, and are calculated detection structure by external equipment and are exported.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510397932.9A CN105137091B (en) | 2015-07-03 | 2015-07-03 | Reagent storing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510397932.9A CN105137091B (en) | 2015-07-03 | 2015-07-03 | Reagent storing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105137091A CN105137091A (en) | 2015-12-09 |
| CN105137091B true CN105137091B (en) | 2017-10-10 |
Family
ID=54722514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510397932.9A Active CN105137091B (en) | 2015-07-03 | 2015-07-03 | Reagent storing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105137091B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5272093A (en) * | 1990-08-02 | 1993-12-21 | Miles Inc. | Reagent containment and delivery tray and method of use |
| CN101408549A (en) * | 2007-10-08 | 2009-04-15 | 因福皮亚有限公司 | Reaction cassette for measuring the concentration of glycated hemoglobin and measuring method thereof |
| CN102620959A (en) * | 2002-12-26 | 2012-08-01 | 梅索磅秤技术有限公司 | Assay cartridges and methods of using the same |
| WO2014159834A1 (en) * | 2013-03-14 | 2014-10-02 | Siemens Healthcare Diagnostics Inc. | Microfluidic chip with sealed on-board reagent |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6949377B2 (en) * | 2001-03-05 | 2005-09-27 | Ho Winston Z | Chemiluminescence-based microfluidic biochip |
| US20050221281A1 (en) * | 2003-01-08 | 2005-10-06 | Ho Winston Z | Self-contained microfluidic biochip and apparatus |
| US8911689B2 (en) * | 2010-07-27 | 2014-12-16 | General Electric Company | Interfacing caps for microfluidic devices and methods of making and using the same |
-
2015
- 2015-07-03 CN CN201510397932.9A patent/CN105137091B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5272093A (en) * | 1990-08-02 | 1993-12-21 | Miles Inc. | Reagent containment and delivery tray and method of use |
| CN102620959A (en) * | 2002-12-26 | 2012-08-01 | 梅索磅秤技术有限公司 | Assay cartridges and methods of using the same |
| CN101408549A (en) * | 2007-10-08 | 2009-04-15 | 因福皮亚有限公司 | Reaction cassette for measuring the concentration of glycated hemoglobin and measuring method thereof |
| WO2014159834A1 (en) * | 2013-03-14 | 2014-10-02 | Siemens Healthcare Diagnostics Inc. | Microfluidic chip with sealed on-board reagent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105137091A (en) | 2015-12-09 |
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