CN105021807B - Reagent reacting box and the application in biology sample detection thereof - Google Patents
Reagent reacting box and the application in biology sample detection thereof Download PDFInfo
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- CN105021807B CN105021807B CN201510395786.6A CN201510395786A CN105021807B CN 105021807 B CN105021807 B CN 105021807B CN 201510395786 A CN201510395786 A CN 201510395786A CN 105021807 B CN105021807 B CN 105021807B
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
Abstract
The present invention relates to detection of biological samples technical field, particularly relate to a kind of reagent reacting box and the application in biology sample detection of the reagent reacting box, the reagent reacting box of the present invention, including reagent storage part, reagent release portion and reacting part, reagent storage part, reagent release portion and reacting part are arranged at inside reaction box；Reagent storage part includes that at least one reagent accommodates chamber, and reagent accommodates chamber and sealed by sealing member；Reagent release portion include one can the push rod of relative reagent storage part motion, push rod is connected with sealing member, and push rod is used for cooperating with external equipment making sealing member separate with reagent storage part；Described reacting part includes at least one reaction zone, and reaction zone receives the reagent of reagent storage part release.The application in biology sample detection of the reagent reacting box of the present invention, including described reagent reacting box.The reagent reacting box of the present invention is a single entirety, it is only necessary to coordinate with detection box, single stepping, can realize the quick release of reagent in reagent storage part.
Description
Technical field
The present invention relates to detection of biological samples technical field, particularly relate to a kind of reagent reacting box and at biological sample
Application in product examine survey.
Background technology
Medical science in-vitro diagnosis plays the part of the role quite reused in medical industry now, and it can be qualitative or quantitative
Measure the change of various Biological indicators in human fluid, to provide the consulting such as medical diagnosis on disease or treatment index, such as,
The detection of the glycolated hemoglobin (HbA1c) in blood for the diagnosis of diabetes and controls most important.
Glycolated hemoglobin is the product that in blood of human body, endoerythrocytic hemoglobin is combined with blood glucose, works as blood
When middle concentration of glucose is higher, the content of the HbA1c that human body is formed also can be of a relatively high.Erythrocyte in human body
Average life be 120 days, before cell death, in blood, the content of HbA1c also can keep relatively the most not
Becoming, therefore, glycolated hemoglobin test generally can reflect patient's glycemic control situation of nearly 8～12 weeks, no
It is raised and lowered by an once in a while blood glucose and is affected.
Prior art also has the multiple concentration being designed for detecting analyte, such as:
As it is shown in figure 1, US1562237 discloses a kind of reaction box, reaction box includes reaction channel and liquid examination
Agent storage part, reaction channel is provided with dry reagent, liquid reagent storage part be used for depositing buffer or other
Liquid reagent, liquid reagent storage part includes storing body 30', and storage body is sealed by sealing member or thin film 32',
Thin film 32' has a far-end 33', directly tears thin film from far-end and makes thin film separate with storage body, will storage
Reagent in body is discharged in reaction channel.
When releasing liquid reagent, need artificially to be separated by the thin film 32' being originally sealed on storage body 30'
Open, then thin film is removed.Although aforesaid way can tear thin film, releasing liquid reagent, but, by one
The far-end 33' of Duan Yanshen tears thin film and is extremely difficult to from storage body 30', during as artificially torn overexertion or
It is firmly to kick the beam easily cause thin film disconnection or tear off not exclusively, makes liquid reagent not discharge completely, detection
Time liquid reagent very few, cause the deviation of testing result；On the other hand, in the non-detection period, thin film 32' is
Exposed aerial, the risk that artificial or other factors is ripped or destroyed easily occurs.
As in figure 2 it is shown, US5272093 discloses one " reagent container and delivering method thereof ", including reagent
Storage chamber 12' and the sealing member 40' being sealed on reagent storage chamber, sealing member is that folding two-layer is arranged, one
Layer stores chamber for sealed reagent, and another layer extends to outside, reagent storage chamber and forms extension 42', extension
42' is for tearing the reagent in release reagent storage chamber.When releasing liquid reagent, needing artificially will be the closeest
The sealing member 40' being enclosed on reagent storage chamber 12' separates, if overexertion or firmly kick the beam as easy as rolling off a log making
Become thin film to disconnect or tear off not exclusively, causing liquid reagent not discharge completely, liquid reagent mistake when making detection
Few, cause the deviation of testing result.
As it is shown on figure 3, US8846380 discloses one " for detecting the reaction box of glycolated hemoglobin concentration ",
Reaction box include the firstth district for accommodating test kit blood sample, for accommodate wash solution the secondth district,
Detection zone and reagent bag, reagent and wash solution in reagent bag are stored separately, and seal up for safekeeping with aluminium foil 120'.When
Time in reagent bag insertion reaction box, aluminium foil is torn by reaction box, and reagent and wash solution in reagent bag are the most temporary
Exist on the first reaction zone and the second reaction zone of reaction box, sent out with blood sample successively by the rotation of reaction box
Raw reaction, thus solve storage and the assignment problem of reagent.
Above-mentioned reaction box, when detection, firstly, it is necessary to artificially fold the release portion 130' on aluminium foil, makes release portion
Clamper in 130' aim detecting box, then the reagent bag after alignment is inserted detection box so that detection box can
Excision release portion, it is achieved aluminium foil separates with reagent bag.This design is relative complex, and operating procedure is various, and
And, need artificial alignment when grafting, easily occur that grafting situation not in place causes grafting repeatedly.The opposing party
Face, reaction box uses split-type design with reagent bag, the opened type design of reaction box, if misoperation, easily
Foreign body is fallen in reaction box, impact the accuracy of testing result.Furthermore, in non-detection period, release
Portion 130' is exposed aerial, the risk that artificial or other factors is ripped or destroyed easily occurs.
Summary of the invention
The technical problem to be solved is for the above-mentioned problems in the prior art, it is provided that a kind of
Reagent reacting box and the application in biology sample detection of the reagent reacting box.
For solving the problems referred to above, a kind of technical scheme of the present invention is:
A kind of reagent reacting box, including reagent storage part, reagent release portion and reacting part, described reagent storage part,
Reagent release portion and reacting part are arranged at inside reaction box；Described reagent storage part includes that at least one reagent holds
Receiving chamber, reagent accommodates chamber and is sealed by sealing member；Described reagent release portion includes that one can the motion of relative reagent storage part
Push rod, push rod is connected with sealing member, and push rod makes sealing member store with reagent for cooperating with external equipment
Part from；Described reacting part includes at least one reaction zone, and reaction zone receives the reagent of reagent storage part release.
Preferably, described reagent storage part includes that multiple reagent accommodates chamber, and multiple reagent accommodate chamber and become array distribution.
Preferably, described reagent storage part includes that at least two row reagent accommodate chamber, and each column includes at least one reagent
Accommodate chamber.
Preferably, during described reagent accommodates chamber, at least one is used for depositing solid particle or powder particle reagent.
Preferably, described reacting part includes the first reaction zone and second reaction zone, the first reaction zone and the second reaction
District receives respectively and temporary different reagent accommodates the reagent that chamber discharges.
Preferably, described first reaction zone is used for keeping in solid particle reagent, the first reaction zone include supporting part and
Stop part, forms a gap between supporting part and stop part, the Breadth Maximum in gap is less than solid particle reagent
Minimum widith, described supporting part is a step.
Preferably, described first reaction zone also includes second baffle, and second baffle is obliquely installed, second baffle with
Stop part forms the second gap, and the minimum widith in the second gap is more than the Breadth Maximum of solid particle reagent.
Preferably, described stop part and second baffle are the baffle plate with radian.
Preferably, described second reaction zone is used for keeping in liquid reagent, and second reaction zone is provided with conducting element, leads
Stream part includes the first drainage plate and the second drainage plate.
The another kind of technical scheme of the present invention is:
The application in biology sample detection of a kind of reagent reacting box, described reagent reacting box includes that reagent stores
Portion, reagent release portion and reacting part, described reagent storage part, reagent release portion and reacting part are arranged at reaction
Inside box；Described reagent storage part includes that at least one reagent accommodates chamber, and reagent accommodates chamber and sealed by sealing member；
Described reagent release portion include one can relative reagent storage part motion push rod, push rod is connected with sealing member, push rod
Sealing member is made to separate with reagent storage part for cooperating with external equipment；Described reacting part includes at least one
Reaction zone, reaction zone receives the reagent of reagent storage part release.
It is compared to prior art, the invention has the beneficial effects as follows:
1. the reagent reacting box of the present invention is a single entirety, and reagent storage part and push rod are all encapsulated in reagent
Inside reaction box, when reaction, it is only necessary to coordinate with detection box, single stepping, will be inserted into by reagent reacting box
In external equipment, the quick release of reagent in reagent storage part can be realized.Said structure is by detection reaction
Artificial manual operation section tapers to the degree simplified most, improves the automaticity of reaction, when reaction,
The most manual operating procedure only includes that sampling rod gathers sample and added to by sample in reagent reacting box and examination
Agent reaction box inserts the step of external equipment.
2. the various piece that the reagent reacting box of the present invention is comprised all is housed in inside reagent reacting box, especially
Reagent storage part and push rod are included in inside reagent reacting box so that the artificial reagent that manually all can not touch stores
Portion and push rod, just can promote when only stretching in reagent reacting box by the external thrust detecting box in external equipment
Push rod action；When having stopped unreacted, because manual operation is improper or other factors, make sealing member part and reagent
Storage part tears off, and causes the possibility that reagent spills in advance.
3. it is all that gap coordinates between the opening on the reagent reacting box of the present invention and each parts, it is provided that reagent
The internal simple reaction compartment of reaction box, it is ensured that the purity of each reaction reagent, it is to avoid foreign is mixed into examination
Possibility in agent reaction box, improves the precision of reaction.
4. the reagent storing device of the present invention is individual components, is encapsulated in reagent reacting box, reagent storing device bag
Include the reagent for depositing solid particle reagent or powder particle reagent and accommodate chamber.Solid particle reagent or powder
Grain reagent is sealed in reagent storing device, it is to avoid random mobile in the reagent reacting box of reagent, it is to avoid existing
The problem needing in technology to be fixed dried reagent, meanwhile, under sealing state, also improves this reagent normal
Effect duration storage life under Wen so that it is be distinguished with other reagent, compares under particularly suitable room temperature and to be difficult to ensure and deposit
Reagent.
5. the reagent storage part of the present invention is provided with latex lyophilizing ball, on the one hand achieves and lives latex antibody response
Property protection maximization, on the other hand, be greatly improved reagent room temperature storage life of lower effect duration.
6. the reagent reacting box of the present invention accommodates number and the row thereof in chamber by configuring reagent in reagent storage part
Row, with coordinating of push rod automation function, it is possible to achieve multiple reagent discharge simultaneously, multiple reagent is according to reaction
Time and order are sequentially or simultaneously added and coordinate drying reagent, complete the possibility of various sample detection.
7. the detecting system of the present invention is when reagent reacting box inserts, it is possible to promote push rod action rapidly, with
Time, quickly consolidated by reagent reacting box is fixed in detection box, it is ensured that detection zone alignment light hole, and i.e. one
Step operation, complete simultaneously reagent release, reagent reacting box fixes and detection zone is to multiple functions such as light, the simplest
Change reactions steps.
Accompanying drawing explanation
Fig. 1 is the structural representation of reaction box in prior art.
Fig. 2 is the structural representation of reagent container in prior art.
Fig. 3 is the partial structurtes schematic diagram of reaction box in prior art.
Fig. 4 is that in detecting system of the present invention, the axle of partial structurtes measures intention.
Fig. 5 be in reagent reacting box of the present invention panel hide after structural representation.
Fig. 6 is the structural representation after reagent reacting box dorsulum of the present invention is hidden.
Fig. 7 is the attachment structure schematic diagram in reagent storage part in the present invention, sealing member and reagent release portion.
Fig. 7 A is the structural representation after sealing member is torn in Fig. 7.
Fig. 8 is the structural representation of reagent storage part in the present invention.
Fig. 8 A is the structural representation of one angle of reagent storage part in the present invention.
Fig. 8 B is the structural representation of another angle of reagent storage part in the present invention.
Fig. 9 is the structural representation of a kind of embodiment of reagent storage part in the present invention.
Figure 10 is the structural representation of reagent storage part another embodiment in the present invention.
Figure 11 is that upper cover in reagent reacting box of the present invention, reagent storage part show with the attachment structure in reagent release portion
It is intended to.
Figure 12 is box body and the attachment structure schematic diagram of push rod in reagent reacting box of the present invention.
Figure 12 A be in reagent reacting box of the present invention push rod relative to box body slide after structural representation.
Figure 13 is the structural representation that in reagent reacting box of the present invention, box body is connected another embodiment with push rod
Figure.
Figure 14 is the axle geodesic structure schematic diagram in release portion in reagent reacting box of the present invention.
Figure 15 is the structure for amplifying schematic diagram in Fig. 6 at A.
Figure 16 is the axle geodesic structure schematic diagram that in reagent reacting box of the present invention, box body is connected with push rod.
Figure 16 A is the structure for amplifying schematic diagram in Figure 16 at C.
Figure 17 is the axle geodesic structure of the another embodiment that box body is connected with push rod in reagent reacting box of the present invention
Schematic diagram.
Figure 18 is the structure for amplifying schematic diagram in Fig. 5 at D.
Figure 19 is that the axle of reagent reacting box external structure of the present invention measures intention.
Figure 19 A is the backsight structural representation of reagent reacting box of the present invention.
Figure 20 is the structural representation of upper cover in reagent reacting box of the present invention.
Figure 20 A is the structure for amplifying schematic diagram in Figure 20 at B.
Figure 20 B is another angled arrangement schematic diagram of upper cover in reagent reacting box of the present invention.
Figure 21 is upper cover and the attachment structure schematic diagram of reagent storage part in reagent reacting box of the present invention.
Figure 22 is the explosive view of reagent reacting box of the present invention.
Figure 23 is the cross-sectional view that the present invention detects box.
Figure 24 is that the present invention detects the structural representation of internal part in box.
Figure 25 is that in reagent reacting box of the present invention insertion detection box, push rod is illustrated by the cross-section structure of push rod jack-up
Figure.
Figure 26 is the structural representation that the present invention detects box Elastic part.
Figure 27 is that the present invention detects the structural representation of shell fragment in box.
Figure 28 is the reagent view being in step 0 during reagent reacting box of the present invention detection.
Figure 28 A-28F is the reagent state signal being in step 1-6 during reagent reacting box of the present invention detection respectively
Figure.
Detailed description of the invention
Further describe the present invention with embodiment below in conjunction with the accompanying drawings, but protection scope of the present invention does not limit
In this.
As shown in Figure 4, the detecting system of the present invention is the concentration of analyte in quickly detection biological sample,
Including reagent reacting box 100 and external equipment, external equipment is the equipment in addition to reagent reacting box 100 body,
Wherein, external equipment also referred to as detects equipment, external equipment or detection equipment and is provided with a detection box 200, examination
Agent reaction box 100 and detection box 200 support the use.Reagent reacting box 100 is as the container of reaction, Ke Yishi
The functions such as the existing storage of plurality of reagents, the order interpolation of plurality of reagents and devil liquor recovery.Detection box 200 is used for putting
Putting reagent reacting box 100, detection box 200 is arranged on detection equipment, and is provided reagent reacting by detection equipment
Box 100 rotates, the machine power of reagent mixing, reagent release etc. and control in reagent reacting box 100, simultaneously
Being additionally provided with optics on external equipment, optics is the concentration of detectable substance in detecting fluid sample.
As shown in Figure 5 and Figure 6, be provided with in reagent reacting box 100 reagent storage part 102, reagent release portion 150,
Reacting part and detection zone 28.Reagent storage part 102 and reagent release portion 150 are all encapsulated in reagent reacting box 100
In.Preferably, being provided with a cavity in described reagent reacting box 100, described reagent storage part 102 is housed in chamber
In body.Reagent storage part 120 is encapsulated in reagent reacting box 100 for individual components, beneficially reagent reacting box
Quick assembling.Reagent storage part 102 is for sealed type storage solid particle, powder or liquid reagent, and reagent is released
Put portion 150 to detect in detection equipment and under the cooperation of box 200, can quickly open reagent storage part 102 release examination
Agent is in the reacting part of reagent reacting box 100.Reacting part for biological sample and reagent temporary, mix and instead
Should, and by detection zone 28, intermediate product or the end product of reaction are detected.
As shown in Figure 7 and Figure 8, reagent storage part 102 includes that at least one reagent accommodates chamber, reagent storage part
102 are sealed by sealing member 104, i.e. reagent receiving chamber is sealed by sealing member 104.Reagent storage part 102 is also referred to as
For reagent storing device, reagent storage part 102 includes that multiple reagent accommodates chamber, and multiple reagent accommodate chamber and become array to divide
Cloth.Reagent accommodates the reagent type required for the quantity in chamber is reacted according to detection, the order of interpolation is set,
Such as one, two or multiple, it is independent each other that reagent accommodates chamber, the most multiple reagent accommodate chamber that
It is provided with spacing between this, becomes array distribution.Such as, it can be shape the most in a row that two reagent accommodate chamber, also
Can be longitudinally to become column-shaped, can also be to angular distribution；For another example, multiple reagent accommodate chamber and can be spaced plug hole and divide
Cloth.It is also that the dosage required for reacting according to detection is set that reagent accommodates the capacity in chamber.
Preferred as one, described reagent storage part includes that at least two row reagent accommodate chamber, and each column includes at least one
Individual reagent accommodates chamber.Preferably, as shown in Figure 8, reagent storage part 102 includes that two reagent accommodate chambeies, two
It is transversely arranged that individual reagent accommodates left and right, chamber.I.e. reagent accommodates chamber 106 and reagent accommodates chamber 108 and arranges a left side in a row two
Parting cloth on the right side, reagent accommodates the bottom in chamber the most in the same horizontal line.Therefore, when tearing sealing member 104,
The reagent that reagent accommodates in chamber 106 and reagent receiving chamber 108 can together be discharged in reagent reacting box 100.
As it is shown in figure 9, as a kind of embodiment of reagent storage part 102, two reagent accommodate chamber longitudinally row
The situation of row, i.e. reagent accommodate chamber 110 and reagent accommodates chamber 112 and is distributed up and down in two row's string, tear in part
When opening sealing member 104, the reagent receiving chamber 112 being positioned at lower row preferentially discharges reagent, according to wanting of response time
Ask, continue to tear sealing member 104, make the reagent being positioned at row accommodate chamber 110 and discharge reagent.Controlling sealing
Under the degree that part 104 is torn, it is possible to be sequentially added into reagent, control the carrying out of reaction.
As shown in Figure 10, as the another embodiment of reagent storage part, it is array that four reagent accommodate chamber
The situation of arrangement, i.e. reagent accommodate chamber 114, reagent accommodates chamber 116, reagent accommodates chamber 118 and reagent accommodates
Chamber 120 is distributed in two row four column-shaped.Under controlling the degree that sealing member 104 is torn, the reagent of lower row accommodates chamber
118 and the reagent that accommodates in chamber 120 of reagent preferentially discharge, the then reagent of the upper row of release.
Preferred as one, described reagent storing device includes for depositing solid particle reagent or powder particle reagent
Reagent accommodate chamber.Solid particle reagent or powder particle reagent is sealed in reagent storing device, it is to avoid reagent
Arbitrarily moving in reagent reacting box, it is to avoid the problem needing in prior art dried reagent is fixed,
Meanwhile, under sealing state, also improve the effect duration storage life under this reagent room temperature so that it is with other reagent area
Do not come, compare under particularly suitable room temperature and be difficult to ensure the reagent deposited.
Preferably, described solid particle reagent or powder particle reagent are lyophilized solid particle reagents or freeze-dried powder
Particle reagents.Preferably, described solid particle reagent is latex lyophilizing ball.Latex lyophilizing ball, on the one hand realizes
Maximization to the protection of latex antibody reactivity, on the other hand, is greatly improved under reagent room temperature effectively
Phase storage life.
Preferred as one, described reagent storing device also includes that the reagent for depositing liquid reagent accommodates chamber.Examination
Agent accommodate chamber may be used for depositing solid particle, powder particle or liquid reagent, can according to detection need into
Row sets.
Preferred as one, as shown in Figure 8 A, at least one reagent accommodates chamber and is provided with injection orifice 130, note
Perforation 130 connects reagent and accommodates chamber and space outerpace, and reagent accommodates chamber and injection orifice 130 by sealing member 104
Seal.Preferably, injection orifice 130 is located at the reagent receiving chamber for depositing powder reagent or liquid reagent.
For eased sealing liquid reagent or powder reagent, when sealing, first with sealing member 104 sealed reagent
Accommodate chamber and outside by exposed for injection orifice 130 then liquid or powder reagent are injected into reagent accommodate in chamber,
Sealing injection hole 130 the most again, ensure that the effect of sealing with this, and reduces sealing liquid or powder reagent
Difficulty.Reagent accommodates chamber and injection orifice can be sealed by same sealing member timesharing substep, can be divided by two sealing members
Do not seal, as used sealing plug sealing injection hole etc..
Preferred as one, as shown in Figure 8 B, the back of reagent storage part 102 is additionally provided with for installing dry
The cavity 132 of drying prescription.When reagent storage part 102 is arranged in reagent reacting box 100, by cavity 132 dough-making powder
The cooperation of plate 10 inwall, desiccant is arranged in cavity 132.
Preferred as one, reagent storage part 102 includes that two reagent accommodate chamber, and two reagent accommodate chamber respectively
For storing solid particle reagent and liquid reagent.With reagent storage part 102, the present embodiment includes that two reagent hold
Receiving chamber 106, be described in detail as a example by 108, reagent accommodates chamber 106 and stores solid particle reagent and reagent accommodates
Chamber 108 stores liquid reagent, and is provided with injection orifice 130 in the upper end in reagent receiving chamber 108.When sealing first
Solid particle reagent is placed on reagent and accommodates in chamber 106, accommodate chamber 106 with sealing member 104 sealed reagent
With the cavity hatch that reagent accommodates chamber 108, then liquid reagent is injected into reagent accommodates in chamber 108, then profit
With sealing member 104 sealing injection hole 130.
Preferred as one, reagent accommodates the bottom design in chamber and takes the form of inclined plane.When discharging reagent, facilitate reagent
Quickly flow out, decrease reagent in the residual accommodating intracavity, it is ensured that the precision that reagent volumes controls as far as possible.
As it is shown in fig. 7, reagent storage part 102 is sealed by sealing member 104, reagent receiving will be sealed in by reagent
In chamber, sealing member 104, under the effect of external force, can accommodate chamber with reagent and tear off, tearing off of sealing member 104
Can be by from left to right, the mode such as from lower to upper, illustrate as a example by sealing member tears off from lower to upper herein,
As shown in Figure 7 A.Sealing member 104 can be conventional use of sealing member in the prior art such as aluminium foil, thin film.
As is illustrated by figs. 11 and 12, reagent release portion 150 includes push rod 152, push rod 152 and sealing member 104
Being connected, described push rod 152 makes sealing member separate with reagent storage part for cooperating with external equipment.Such as figure
Shown in 7A, i.e. sealing member 104 is pasted or is fixed on push rod 152, when external device acts on push rod 152
Time, push rod 152 coordinates the generation motion relative to reagent storage part 102 with the inwall of reagent reacting box 100,
While push rod 152 moves, band dynamic seal 104 relative reagent storage part 102 moves, i.e. sealing member
104 actions torn off so that the reagent that reagent accommodates in chamber is discharged in reagent reacting box 100.Push rod 152
The motion of relative reagent storage part 102 can to serve as reasons from left to right, the right side to turn left, from lower to upper etc. mode realize.
Preferred as one, as it is shown in fig. 7, one end sealed reagent storage part 102 of sealing member 104, another
End is pasted onto on push rod 152 after folding.
Preferred as one, described push rod 152 is provided with the forced section cooperated with external equipment.Forced section
For accepting the thrust that external equipment provides.
Preferred as one, it is internal that push rod 152 is all housed in reagent reacting box 100, the thrust of external equipment
Part needs extend into reagent reacting box 100 inside or act on the forced section of push rod 152 by other means.Push away
Bar 152 is all housed in reagent reacting box, and human contact is less than the push rod in reagent reacting box, the most free-hand
Push rod 152 action can not be promoted, after by external tool, only just can make push rod 152 action.Thus
Avoiding in the non-detection period, maloperation or other reason are artificially torn or breakseal part 104 causes reagent to carry
The possibility of front leakage.
Preferred as one, as shown in figure 13, in reagent reacting box 100, it is provided with chute 38.On the one hand, sliding
Push rod 152 is embedded in slideway by groove 38, and on the other hand, chute 38 is slidably matched with push rod 152.As one
Individual embodiment, chute 38 is arranged on backboard 12, and chute 38 can be designed as only backboard 12 and two grooves
Limit forms, and now, push rod 152 dual-side partial receipt is in chute 38.
Preferred as one, as shown in Figure 12 and Figure 12 A, described chute 38 is provided with division board 36, every
The inner space of chute 38 and reagent reacting box 100 is isolated from plate 36.As a kind of embodiment, chute
38 is the column of at least one end open, and four sides of column are by part backboard 12, chute 38 dual-side
Forming with division board 36, say, that chute 38 is tightly connected division board 36, division board 36 separates chute
38 will not flow to reagent reacting box with the inner space of reagent reacting box 100, division board 36 height with reactant liquor
Outer for design requirement.Chute 38 is designed to an independent region, by push rod 152 or external thrust part and examination
Agent is kept apart, and push rod 152 or external thrust part can be avoided to take away portion of reagent with contacting of reaction reagent.
Preferred as one, as shown in figure 14, at least one side of described push rod 152 is provided with at least one
Individual spacing preiection 154.Spacing preiection 154 can make push rod 152 more stable be arranged on reagent reacting box 100
Inside.Preferably, the quantity of spacing preiection 154 is two, is separately positioned on the left and right side of push rod 152
On.Preferably, described spacing preiection 154 is arranged on the top of push rod 152 side.When push rod 152 is arranged on
Time in reagent reacting box 100, the spacing preiection 154 on push rod 152 under the extruding of reagent reacting box 100,
Make push rod 152 produce elastic deformation, add the frictional force of push rod 152 and reagent reacting box 100, make push rod
152 can be stable be arranged in reagent reacting box 100, also increase the push rod 152 resistance when moving.
Preferred as one, as shown in figure 14, described push rod 152 is provided with at least one hollow out near edge
Cell body 156, described spacing preiection 154 is arranged on the lateral wall of cell body 156, spacing preiection 154 and groove
Body 156 is arranged in pairs.As a specific embodiment, spacing preiection 154 is two, two spacing preiections
On 154 left and right sides being separately positioned on push rod 152, corresponding, the cell body 156 of hollow out is also two
Individual, it is separately positioned on the left of push rod and right side is push rod near edge, the i.e. lateral wall of left side cell body 156
The left surface of 152, the lateral wall of right edge cell body 156 is the right flank of push rod 152, say, that spacing
Protruding 154 are arranged on the lateral wall of cell body 156.The setting of hollow out cell body 156, when making push rod 152 action
More it is easily generated deformation, it is to avoid cause push rod 152 to slide because friction is excessive dumb.
If push rod 152 is chronically at deformed state and is easy for losing certain elasticity, and makes fastening effect the best,
For solving the problems referred to above, preferred as one, as shown in figure 15, reagent reacting box 100 is provided with spacing
Protruding 154 limited impressions 158 coordinated.Preferably, chute 38 is provided with and blocks with described spacing preiection 154
The limited impression 158 closed, the quantity of limited impression 158 is identical with spacing preiection 154.Push rod 152 is initially
Time under state i.e. normality, limited impression 158 engages with spacing preiection 154, and now, push rod 152 does not occurs shape
Become；When the push rod 152 promotion lower slider in external force, push rod 152 produces deformation also at spacing preiection 154
Leave limited impression 158.
Preferred as one, as shown in figure 14, described push rod is provided with a groove 162.Groove 162 is arranged
In the upper end of push rod 152, groove 162, for pasting groove, pastes groove for adhesive seal part 104.Do not pasting
Before sealing member, the bottom land place plane of groove 162 is slightly below groove 162 notch place plane, works as adhesive seal
After part 104, sealing member 104 fills and leads up groove 162, makes push rod plane concordant.When avoiding push rod 152 to promote,
The possibility of stripping is caused because sealing member 104 exceeds push rod 152 surface.
Preferred as one, the forced section of push rod 152 is bottom surface or the back of push rod, when forced section is push rod
During bottom, External Force Acting, in the bottom surface of push rod, makes push rod 152 tear sealing member 104 from lower to upper, works as stress
When portion is the back of push rod, External Force Acting, in the back of push rod, promotes push rod 152 to tear from lower to upper close equally
Sealing 104.The forced section of push rod 152 can also be the upper bottom surface of push rod, left surface or right flank, for upper
During bottom surface, push rod can be pulled from top；During for left surface, push rod can be promoted from left to right；For right flank
Time, promotion push rod of can turning left from the right side.
As another embodiment, the forced section of push rod 152 is connected with extraneous thrust piece magnetic, i.e. push rod
Forced section, the extraneous thrust piece of 152 are the magnet assembly attracted each other, such as iron block, Magnet etc..Push rod 152
Isolated by reagent reacting box 100 with extraneous thrust piece, under magnetic action, extraneous thrust piece drive push rod
152 actions.
Preferred as one, reagent reacting box 100 is provided with an opening, and forced section is exposed in said opening,
Forced section receives external thrust by opening.I.e. external thrust part can stretch in opening, with reagent reacting box
Push rod 152 in 100 contacts and promotes its action, and the forced section that aperture position is arranged with push rod 152 arranges phase
Close.
As a kind of embodiment, as shown in figure 16, the forced section of push rod 152 is the bottom surface of push rod, aforementioned
Opening 34 be arranged on the base plate 4 of reagent reacting box 100.Preferably, as shown in Figure 16 A, opening 34
Being provided with chamfering 40, push rod 152 is all housed in chute 38 and distance opening 34 has certain interval,
To avoid collision during maloperation.External thrust part is introduced and stretches into chute 38 by the chamfering 40 of opening 34,
Contact with push rod 152, and promote push rod 152 and external thrust part to be slidably matched with chute 38.
As another embodiment, as shown in figure 17, the forced section of push rod 152 is the back of push rod 152,
Aforementioned opening 34 is then arranged on the backboard 12 of reagent reacting box 100.Preferably, on the back of push rod 152
Being provided with fin or recess 160, fin or recess 160 is forced section, and fin or recess 160 are exposed at opening 34
In.External thrust part contacts with fin or recess 160, and promotes push rod 152 to be slidably matched with chute 38.
Described reacting part includes at least one reaction zone, and reaction zone receives the reagent of reagent storage part 102 release.
Reacting part includes multiple reaction zone, and the quantity of reaction zone and position set and hold according to reagent in reagent storage part 102
Receiving the quantity in chamber and reactions steps is correlated with, two kinds of reagent as discharged simultaneously can be temporarily stored in a reaction zone,
Can also be temporarily stored in respectively in two each independent reaction zones；If also drying reagent in reaction zone, it is possible to individually
Other reaction zone is set, is interconnected between each reaction zone, can be realized each by rotating reagent reacting box
The mixing of reagent in reaction zone.
As shown in Figure 5 and Figure 6, being provided with detection zone 28 in reacting part, detection zone 28 can be arranged on reagent
On the circulation passage of any agent of reaction box 100, detection zone 28 typically uses transparent material, makes optics device
Transmission light or scattered light that part sends enter reagent reacting box 100.Set between reagent storage part 102 and reacting part
Having conducting element, more specifically, conducting element is arranged on reagent and accommodates between chamber and corresponding reaction zone, water conservancy diversion
The reagent that part makes reagent accommodate intracavity is discharged in reaction zone fast and accurately.
Preferred as one, as shown in Fig. 5 and Figure 18, reacting part includes at least one reaction zone, Qi Zhongzhi
A few reaction zone is the first reaction zone 26, and described first reaction zone 26 is used for keeping in solid particle reagent, the
One reaction zone 26 includes supporting part and stop part, forms a gap 47, gap 47 between supporting part and stop part
Breadth Maximum less than the minimum widith of solid particle reagent.Gap between supporting part and stop part is used for stopping
Solid particle reagent in first reaction zone enters other reaction zone, and make that solid particle reagent can be stable is temporary
On supporting part.As a kind of embodiment, supporting part is a step 46, and stop part is a baffle plate, is designated as
First baffle plate 48.First baffle plate 48 is vertical baffle plate, and the bottom of the first baffle plate 48 forms seam with supporting part
Gap 47.Preferably, described solid particle reagent is latex lyophilizing ball agent.The minimum of latex lyophilizing ball agent
The width i.e. diameter of latex lyophilizing ball, more than the Breadth Maximum in gap 47, makes latex lyophilizing ball agent by gap 47
Stop.
Preferred as one, as shown in Fig. 5 and Figure 18, the first reaction zone 26 also includes second baffle 50,
Second baffle 50 is obliquely installed, and second baffle 50 and stop part form the second gap 49, the second gap 49
Minimum widith is more than the Breadth Maximum of solid particle reagent.Second baffle 50 tries for solid particle with stop part
Agent drain, enables solid particle reagent to smoothly enter in the first reaction zone 26.As a concrete reality
Executing mode, the Breadth Maximum of the latex lyophilizing ball agent i.e. diameter of latex lyophilizing ball is less than the second gap 49
Little width, makes in the convenient entrance of latex lyophilizing ball agent the first reaction zone 26.
Preferred as one, the first baffle plate 48 and second baffle 50 are the baffle plate with radian.On the one hand make solid
Body particle reagents can rapidly enter, it is contemplated that the difference on Solids Particle Diameter, it is to avoid solid particle is stuck in
Cannot fall on step 46 between one baffle plate 48 and second baffle 50.
Preferred as one, described reacting part also includes second reaction zone, and second reaction zone is used for keeping in liquid examination
Agent.Preferably, second reaction zone is provided with conducting element, and conducting element includes the first drainage plate and the second drainage plate.
As a kind of detailed description of the invention, as shown in Figure 5 and Figure 6, reacting part includes the first reaction zone 26 He
Second reaction zone 25, the first reaction zone 26 accommodates the solid particle of chamber 106 release for receiving reagent, and second
Reaction zone 25 accommodates the liquid reagent of chamber 108 release for receiving reagent.Second reaction zone 25 is provided with water conservancy diversion
Part, conducting element includes the first drainage plate 42 and the second drainage plate 44, makes liquid reagent flow rapidly into the second reaction
In district 25.First reaction zone 26 includes step the 46, first baffle plate 48 and the second baffle for keeping in reagent
50, the first baffle plate 48 and second baffle 50 are used for guiding solid particle to enter step 46, meanwhile, first gear
Forming gap 47 between plate 48 and step 46, this gap 47 can stop solid particle to enter second reaction zone
25, and allow liquid reagent to flow in the first reaction zone 26.
As shown in Figure 19 and Figure 19 A, reagent reacting box 100 includes top board 2, base plate 4, left plate 6, the right side
Side plate 8, panel 10 and backboard 12.The substantially square box-like body of reagent reacting box 100, by plastic material system
Become,
Preferred as one, as shown in figure 19, the left plate 6 of described reagent reacting box 100 or right plate 8
One end be an inclined-plane 22.Preferably, the backboard 12 of reagent reacting box 100 is provided with an extension 24, prolongs
Extending portion 24 extends out to the outside on inclined-plane 22.Preferably, inclined-plane 22 is arranged on a left side for reagent reacting box 100
Inferior horn.In one embodiment, backboard 12 is square, and panel 10 is pentagon, and left plate 6 has one
Side and an inclined-plane, the rectangular triangle of extension 24, the hypotenuse of right angled triangle is backboard 12 and extension
The junction in portion 24.Inclined-plane 22 and the setting of extension 24, it is simple to artificial differentiation reagent reacting box 100
Positive and negative, it is to avoid reagent reacting box 100 is reversely inserted in detection box 200, on the other hand, at reagent reacting
When box coordinates with detection box, can the positive and negative of identification agent reaction box automatically, it is to avoid maloperation.
Preferred as one, as shown in figure 20, reagent reacting box 100 is provided with positioning convex 16.Convex
Playing 16 settings on the faceplate 10, positioning convex 16 is in upside-down triangle shape, and such as Figure 20 A, positioning convex 16 is used
In being fixedly positioning by reagent reacting box 100 in the groove that detection box 200 is corresponding, positioning convex 16 is with recessed
The spacing engaging of groove, makes reagent reacting box 100 be fastened in detection box 200, it is to avoid during rotation, reagent reacting box
100 displacements relatively detecting box 200.
Preferred as one, as shown in Figure 19 A, the top board 2 of reagent reacting box 100 is provided with handle 14,
When detection, facilitate user to hold handle 14 and quickly reagent reacting box 100 is inserted in detection box 200,
After having detected, quickly reagent reacting box 100 is extracted again.Preferred as one, reagent reacting box 100
Backboard 12 be provided with notch 52, the setting of notch 52 makes the injection mo(u)lding of reagent reacting box 100 the most square
Just.
As a kind of detailed description of the invention, as shown in Fig. 5, Fig. 6 and Figure 22, set in reagent reacting box 100
Reagent storage part 102, reagent release portion 150, reacting part, detection zone 28, sampling rod 30 and liquid is had to inhale
Receive pad 32, reagent reacting box 100, reagent storage part 102, reagent release portion 104 and sampling rod 30 by
Plastic material is made.Sampling rod 30 is used for gathering fluid sample such as blood, urine etc., and is added by fluid sample
Enter in reagent reacting box 100.Upon completion of the assays, liquid absorption pad 32 is for Recycling of waste liquid, it is to avoid liquid
Body leakage pollutes.
As shown in Fig. 5, Fig. 6 and Figure 22, reagent reacting box 100 is a cavity, can be divided into box body 18 and upper
Lid 20, box body 18 includes backboard 12, base plate 4, left plate 6 and right plate 8, and upper cover 20 includes top board 2
With panel 10, upper cover 20 and box body 18 are tightly connected by the mode such as welding, facilitate reagent reacting box 100
Assembling.Described liquid absorption pad 32 is arranged on the top on inclined-plane 22, and liquid absorption pad 32 is turned right successively
The direction of plate 8 is sampling rod 30 and reagent storage part 102, and reacting part and reagent release portion 104 are arranged at
The lower section of reagent storage part 102, detection zone 28 is arranged in reacting part.Sampling rod 30 is arranged by an opening
In reagent reacting box 100, sampling rod 30 coordinates with this opened gap, it is to avoid during sampling, being mixed into of foreign body.
Inclined-plane 22 is arranged on the left of sampling rod 30, and the beneficially sample on sampling rod 30 is fully contacted with reactant liquor,
Avoid reactant liquor very few and do not reach sample.Liquid absorption pad 32, reagent storage part 102, sampling rod 30,
The general layout in reacting part and reagent release portion 104 is arranged and is not limited to upper type.Preferably, the second drainage plate 44 He
First baffle plate 48 upper end is connected, and the upper end of the first drainage plate 42 is also associated with sampling needle guide plate 57, sampling
Pin guide plate 57 vertical direction is arranged.As shown in Figure 12 and Figure 20 B, box body 18 and upper cover 20 are equipped with and adopt
Sample pin fixture 59, for sampling needle fixture 59, it is to avoid it rocks.
As a kind of detailed description of the invention, reagent storage part and reagent reacting box connected mode, as shown in Figure 8 A,
Reagent storage part 102 is provided with keeper, and each keeper constitutes cavity, and this cavity is used for housing reagent storage
Portion 102 so that reagent storage part 102 is fixedly mounted in reagent reacting box 100.Described cavity can also be adopted
By routine techniques means of the prior art.As a specific embodiment, such as Figure 12 and 12A, Tu20He
Shown in Figure 20 B, panel 10 is provided with locating dowel 54 and gripper shoe 61, left positioner plate 56 and right positioner
Plate 58 is multiple scattered keepers, and scattering device is on box body 18 and upper cover 20, and above-mentioned keeper is used for
Fixing connection reagent storage part 102.As shown in Figure 8 A and 8 B, the keeper of reagent storage part 102 includes
Installing hole 122, left keeper 124, right keeper 126 and lower keeper 128.As shown in figure 21, install
Hole 122 is buckled in locating dowel 54, for the upper end of fixating reagent storage part 102；Left keeper 124 and the right side
Keeper 126 respectively with left positioner plate 56 and right positioner plate 58 is spacing is connected, lower keeper 128 is placed
In gripper shoe 61.Therefore, in reagent storage part 102 is stably mounted at reagent reacting box 100, it is to avoid
The reagent storage part 102 displacement during reagent reacting box 100 moves or rocks.
Above-mentioned each preferably and can the most arbitrarily be matched and combine between embodiment, to realize
The purpose of final quick sample Concentration Testing.
As shown in figure 23 and figure 24, being provided with a push rod 204 in detection box 200, push rod 204 is anti-by reagent
Answer described opening on box 34 to contact with forced section and the active force of external equipment is provided.
Preferred as one, described push rod 204 can relatively detect box 200 and move.Push rod 204 can fix peace
On box body, it is also possible to box body relative motion.When push rod 204 is movable, by peripheral equipment control push rod 204
Zone of action and the position of movement, such as can with motor control push rod 204 movement, it is possible to use existing
Routine techniques in technology.If push rod 204 is fixed on box body, reagent reacting box 100 inserts external equipment
During detection box 200, by insertion force, push rod 204 is coordinated with push rod 152, make push rod 152 action.
Preferred as one, described push rod 204 is arranged on the base plate of detection box 200 or described push rod 204
It is arranged in the inner panel of detection box 200.As shown in figure 25, detection box 200 and forced section are push rod 152
Bottom surface time, opening 34 is arranged on the base plate 4 of reagent reacting box 100, and push rod 204 is arranged on inspection
Survey on the base plate of box 200.Preferably, the upper end of push rod 204 is provided with chamfering, facilitates push rod 204 to stretch into out
Mouth 34.When detection box 200 coordinates with the back that forced section is push rod 152, it is anti-that opening 34 is then arranged on reagent
Answering on the backboard 12 of box 100, push rod 204 is arranged in the inner panel of detection box 200.
Preferred as one, as shown in Figure 24 and Figure 26, in detection box 200, it is provided with a portable plate, portable plate
Including substrate 206 and elastic component 210, substrate 206 is fixedly connected on detection box 200 by elastic component 210
On inwall.Preferably, the quantity of elastic component 210 is two, and elastic component 210 includes installed surface 212 and two
Elastic arm 214, installed surface 212 is fixing with the medial surface of detection box 200 to be connected, two elastic arms 214 respectively with base
Plate 206 is fixing to be connected.
Preferred as one, described substrate 206 is heating plate, is designated as the first heating plate.I.e. portable plate i.e. conduct
Heating member is simultaneously again as elastic fastener.
Preferred as one, one of them medial surface of the most described detection box 200 is provided with bullet
Sheet 216.Preferably, shell fragment 216 is arranged on one of them medial surface adjacent with portable plate.Shell fragment 216
Being provided with an elastic arm 218, elastic arm 218 one end is fixed with shell fragment 216, and the other end of elastic arm 218 is smooth curved surface
Shape, elastic arm 218 is installed towards detection box 200 internal cavities.Shell fragment 216 is in order to reagents reaction box 100
On inclined-plane 22, make the side being not provided with inclined-plane on recoil case 100 be close to detect box 200 medial wall.
Owing to shell fragment 216 has certain elasticity, if reagent reacting box 100 is reversely inserted in detection box 200,
Due to the effect of shell fragment 216, reagent reacting box 100 cannot plug together completely in detection box 200, therefore, and bullet
The cooperation on sheet 216 and inclined-plane 22 can play again the recognition reaction that reagent reacting box 100 is correctly inserted into.
Preferred as one, as shown in figure 23, one of them medial surface of described detection box 200 is provided with recessed
Groove 202.Preferably, groove 202 is arranged on the medial surface of relative motion plate.Groove 202 tries for clamping
Positioning convex 16 on agent reaction box 100, makes reagent reacting box 100 can be connected on groove 202, keeps away
Exempt from detection box 200 rotating or in vibration processes, the displacement of reagent reacting box 100.
As a kind of detailed description of the invention, as shown in Fig. 4, Figure 23 and Figure 24, outside detection box 200 is fixed on
On portion's equipment, to realize mixing or to rotate, detection box 200 includes the box body of open at one end, and box body is provided with logical
Unthreaded hole 201, is provided with push rod the 204, first heating plate 206 and the second heating plate 208, the first heating in box body
Plate 206 is portable plate, and push rod 204 is contacted with forced section by above-mentioned opening 34 and provides external thrust, the
One heating plate 206 and the second heating plate 208 are used for adding thermal agent, to meet the requirement of reaction temperature.
As shown in figure 25, when reagent reacting box 100 is inserted in detection box 200, reagent reacting box 100
Pressing the first heating plate 206, the first heating plate 206 under pressure, makes elastic component 210 deform upon,
Reagent reacting box 100 can quick insertion being fastened in groove 202 by positioning convex 16.Meanwhile, reagent
The inclined-plane 22 of reaction box 100 compresses shell fragment 216, makes detection zone 28 aim detecting on reagent reacting box 100
Light hole 201 on box 200.During reagent reacting box 100 is inserted into detection box 200, push rod 204
Stretch into opening 34 to contact with push rod 152, along with reagent reacting box 100 from top to bottom during, push rod 152
Action from lower to upper, band dynamic seal 104 action from lower to upper, make sealing member 104 and reagent storage part 102
Tearing off, release reagent is as shown in Figure 7 A.
On existing market face, glycolated hemoglobin detection method is a lot, and wherein conventional detection method has ion to exchange
Chromatography, affinity chromatograph, high-pressure liquid phase, immunization, Ion capture, electrophoresis method etc..Immunization refer to red carefully
After cellular lysis, HbA1c is measured in interaction based on antigen molecule and distinct antibodies.
Following two detecting step is included: detect the total blood in sample respectively with immune agglutination method detection HbA1c
Lactoferrin Hb concentration and Glycohemoglobin HbA1c concentration.The detection of total hemoglobin (Hb) is by using
Ferrous ion in potassium ferricyanide oxygenated haemoglobin, generates metahemoglobin, metahemoglobin and sulfur cyanogen
Hydrochlorate reaction generates Hydrogen thiocyanate metahemoglobin, detection light absorption value under 531nm, obtains the concentration of Hb.
Glycolated hemoglobin (HbA1c) with in agglutinin and the blood containing multiple HbA1c immunoreation binding sites
The anti-HbA1c antibody that is marked on latex beads of HbA1c competition binding, the former combination can cause reaction
The change of liquid turbidity, can obtain the concentration of HbA1c in blood by the light absorption value change of detection 531nm.
In blood, the concentration of HbA1c is the highest, and turbidity is the lowest, and light absorption value is the least, light absorption value and the change of HbA1c concentration
Change and obtained by calibration curve.
Therefore, immunization detection HbA1c need use rhodanate liquid reagent (Buffer), marked anti-
The latex balloon of HbA1c antibody, potassium ferricyanide dried reagent (drying object) and containing multiple HbA1c immunoreation
The agglutinin dried reagent (drying object) of binding site.As shown in figure 28, rhodanate liquid reagent 60 is deposited
Storing up and accommodate chamber 108 at reagent, the latex balloon 62 that marked anti-HbA1c antibody is stored in reagent receiving chamber 106
In, potassium ferricyanide dried reagent 64 is solidificated on second reaction zone 25, and agglutinin dried reagent 66 solidifies
In one reaction zone 26.
Wherein, latex balloon is latex lyophilizing bead, will be connected by covalently bound method in advance in latex bead
Upper specificity HbA1c antibody, then use freeze drying technology by bead that latex balloon quick-freezing is that volume is identical, it is achieved
Maximization to the protection of latex antibody reactivity, meanwhile, is greatly improved room temperature storage life of lower effect duration.
Utilize the concentration of HbA1C in the reagent reacting box detection blood of the present invention.Immunization detection HbA1c's
Step is as follows:
Step 0: as shown in figure 28, is inserted into the sampling rod 30 with sample in reagent reacting box.
Step 1: as shown in Figure 28 A, rotates detection box, is inserted into by reagent reacting box in detection box, push rod
152 move upward under the effect of external thrust, tear off sealing member 104, latex balloon 62 and rhodanate 60
Discharging, latex balloon 62 falls through in the first reaction zone 26 temporary, and rhodanate liquid reagent 60 falls simultaneously
In lower entrance second reaction zone 25 in detection zone 28.
Step 2: as shown in Figure 28 B, rotates reagent reacting box, by Buffer60 (rhodanate), drying object
64 (potassium ferricyanides) and blood sample mixing, form mixture X.
Step 3: as shown in fig. 28 c, rotates reagent reacting box, rotates the mixture X in step 2 to inspection
Survey district 28, the content of hemoglobin (Hb) in detection sample.
Step 4: as shown in fig. 28d, continues to rotate reagent reacting box, makes the mixture X in step 2 enter
First reaction zone 26, mixture and the drying object 66 (coagulation containing multiple HbA1c immunoreation binding sites
Element) and latex balloon 62 (marked the latex beads of anti-HbA1c antibody) mixing, form mixture Y.
Step 5: as shown in Figure 28 E, rotates reagent reacting box, makes the mixture Y in step 4 enter detection
District 28, the content of glycolated hemoglobin (HbA1C) in detection sample.
Step 6: as shown in Figure 28 F, rotates reagent reacting box, makes the mixture Y in step 4 enter liquid
Absorption pad 32, makes reacted waste liquid be absorbed.
HbA1c detection terminates, external equipment calculate detection structure and export.
The reagent reacting box of the present invention is not limited to the detection of HbA1c in above-mentioned blood sample, it is also possible to for other
The detection of biological specimen such as urine, saliva, spinal fluid etc. is it can also be used to C reactive protein, gallbladder in biological specimen
The Concentration Testing of the analytes such as sterin, blood fat and blood glucose.
In described above, all that do not add special instruction, all use routine techniques means of the prior art.
Claims (11)
1. a reagent reacting box, including reagent storage part, reagent release portion and reacting part, it is characterised in that institute
State reagent storage part, reagent release portion and reacting part to be arranged at inside reaction box；
Described reagent storage part includes that at least one reagent accommodates chamber, and reagent accommodates chamber and sealed by sealing member；
Described reagent release portion include one can relative reagent storage part motion push rod, push rod and sealing member phase
Even, push rod makes sealing member separate with reagent storage part for cooperating with external equipment；
Described reacting part includes at least one reaction zone, and reaction zone receives the reagent of reagent storage part release.
Reagent reacting box the most according to claim 1, it is characterised in that described reagent storage part includes multiple
Reagent accommodates chamber, and multiple reagent accommodate chamber and become array distribution.
Reagent reacting box the most according to claim 2, it is characterised in that described reagent storage part includes at least
Two row reagent accommodate chamber, and each column includes that at least one reagent accommodates chamber.
4. according to the reagent reacting box described in any one in claim 13, it is characterised in that described reagent holds
Receive in chamber at least one for depositing solid particle reagent.
Reagent reacting box the most according to claim 3, it is characterised in that described reacting part includes the first reaction
District and second reaction zone, the first reaction zone and second reaction zone receive respectively and temporary different reagent accommodates chamber
The reagent of release.
Reagent reacting box the most according to claim 5, it is characterised in that described first reaction zone is used for keeping in
Solid particle reagent, the first reaction zone includes supporting part and stop part, is formed between supporting part and stop part
One gap, the Breadth Maximum in gap is less than the minimum widith of solid particle reagent, and described supporting part is one
Rank.
Reagent reacting box the most according to claim 6, it is characterised in that described first reaction zone also includes
Two baffle plates, second baffle is obliquely installed, and second baffle and stop part form the second gap, the second gap
Minimum widith is more than the Breadth Maximum of solid particle reagent.
Reagent reacting box the most according to claim 7, it is characterised in that described stop part and second baffle are
There is the baffle plate of radian.
9. according to the reagent reacting box described in claim 58 any one, it is characterised in that described second reaction
District is used for keeping in liquid reagent, and second reaction zone is provided with conducting element, conducting element include the first drainage plate and
Second drainage plate.
Reagent reacting box the most according to claim 4, it is characterised in that described solid particle reagent is powder
Particle reagents.
The application in biology sample detection of 11. 1 kinds of reagent reacting boxes, it is characterised in that
Described reagent reacting box includes reagent storage part, reagent release portion and reacting part, and described reagent stores
Portion, reagent release portion and reacting part are arranged at inside reaction box；
Described reagent storage part includes that at least one reagent accommodates chamber, and reagent accommodates chamber and sealed by sealing member；
Described reagent release portion include one can relative reagent storage part motion push rod, push rod and sealing member phase
Even, push rod makes sealing member separate with reagent storage part for cooperating with external equipment；
Described reacting part includes at least one reaction zone, and reaction zone receives the reagent of reagent storage part release.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510395786.6A CN105021807B (en) | 2015-07-03 | 2015-07-03 | Reagent reacting box and the application in biology sample detection thereof |
| PCT/CN2016/087523 WO2017005124A1 (en) | 2015-07-03 | 2016-06-28 | Reagent reaction box and application thereof in biological sample testing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510395786.6A CN105021807B (en) | 2015-07-03 | 2015-07-03 | Reagent reacting box and the application in biology sample detection thereof |
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| Publication Number | Publication Date |
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| CN105021807A CN105021807A (en) | 2015-11-04 |
| CN105021807B true CN105021807B (en) | 2016-08-31 |
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| CN201510395786.6A Active CN105021807B (en) | 2015-07-03 | 2015-07-03 | Reagent reacting box and the application in biology sample detection thereof |
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| Country | Link |
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| CN (1) | CN105021807B (en) |
| WO (1) | WO2017005124A1 (en) |
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|---|---|---|---|---|
| CN105021544B (en) | 2015-07-03 | 2018-02-02 | 艾康生物技术(杭州)有限公司 | Detecting system |
| CN105013546B (en) * | 2015-07-03 | 2017-12-08 | 艾康生物技术(杭州)有限公司 | Biological sample reaction box |
| CN105021807B (en) * | 2015-07-03 | 2016-08-31 | 艾康生物技术(杭州)有限公司 | Reagent reacting box and the application in biology sample detection thereof |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5162237A (en) * | 1988-04-11 | 1992-11-10 | Miles Inc. | Reaction cassette for preforming sequential analytical assays by noncentrifugal and noncapillary manipulations |
| CA2043464C (en) * | 1990-08-02 | 2001-01-30 | James J. Silva | Reagent containment and delivery tray |
| US5272093A (en) * | 1990-08-02 | 1993-12-21 | Miles Inc. | Reagent containment and delivery tray and method of use |
| CA2772050C (en) * | 2002-12-26 | 2016-09-06 | Meso Scale Technologies, Llc. | Assay cartridges and methods of using the same |
| KR100798471B1 (en) * | 2007-10-08 | 2008-01-28 | 주식회사 인포피아 | Reaction cassette for measuring glycated hemoglobin and measuring method thereof |
| CN102200536B (en) * | 2010-03-25 | 2015-05-27 | 艾博生物医药(杭州)有限公司 | Device for detecting analyzed objects in test liquid samples |
| CN202903544U (en) * | 2012-08-30 | 2013-04-24 | 苏爱美 | Urine collector for clinical examination |
| TWI484182B (en) * | 2012-11-07 | 2015-05-11 | Taidoc Technology Corp | Reaction vessel, assay device, and measuring method |
| CN103278625A (en) * | 2013-04-28 | 2013-09-04 | 上海快灵生物科技有限公司 | Closed type chromatography test paper detection device and application method of closed type chromatography test paper detection device |
| CN204832049U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Detect box |
| CN105013546B (en) * | 2015-07-03 | 2017-12-08 | 艾康生物技术(杭州)有限公司 | Biological sample reaction box |
| CN105021807B (en) * | 2015-07-03 | 2016-08-31 | 艾康生物技术(杭州)有限公司 | Reagent reacting box and the application in biology sample detection thereof |
| CN204816573U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Biological sample reaction box |
| CN204832204U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Reagent reaction box |
| CN105021544B (en) * | 2015-07-03 | 2018-02-02 | 艾康生物技术(杭州)有限公司 | Detecting system |
| CN205049571U (en) * | 2015-07-03 | 2016-02-24 | 艾康生物技术(杭州)有限公司 | Reaction box |
| CN204855358U (en) * | 2015-07-03 | 2015-12-09 | 艾康生物技术(杭州)有限公司 | Assay system |
| CN204832194U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Biological sample testing cassette |
| CN204855520U (en) * | 2015-07-03 | 2015-12-09 | 艾康生物技术(杭州)有限公司 | Biological sample analysis box |
-
2015
- 2015-07-03 CN CN201510395786.6A patent/CN105021807B/en active Active
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2016
- 2016-06-28 WO PCT/CN2016/087523 patent/WO2017005124A1/en active Application Filing
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| Publication number | Publication date |
|---|---|
| CN105021807A (en) | 2015-11-04 |
| WO2017005124A1 (en) | 2017-01-12 |
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