CN105123765A

CN105123765A - Preparing method for chitin copper-lactate gray-mold preventing and treating preparation

Info

Publication number: CN105123765A
Application number: CN201510643437.1A
Authority: CN
Inventors: 王猛
Original assignee: Suzhou Keda Weilong Agricultural Technology Co Ltd
Current assignee: Suzhou Keda Weilong Agricultural Technology Co Ltd
Priority date: 2015-10-08
Filing date: 2015-10-08
Publication date: 2015-12-09

Abstract

The invention discloses a preparing method for a chitin copper-lactate gray-mold preventing and treating preparation. Materials for the preparing method for the chitin copper-lactate gray-mold preventing and treating preparation are prepared from 80% of plant lactic acid bacterium liquid, 15% of chitin and 5% of nanometer complexing monovalent copper. The preparing method includes the steps that 1, tender leaves of towel gourds are collected to be mixed with brown sugar in the ratio of 2:1, the mixture is placed into a fermentation kettle to be fermented for 7 d, and a suspension obtained after filtering is the plant lactic acid bacterium liquid; 2, the nanometer complexing monovalent copper is slowly put into the suspension obtained in the step 1 till the suspension gets into a cyan color; 3, the chitin is further put into the liquid obtained after the step 2 is completed, stirring is carried out, then standing is carried out for 24 d, and obtained viscous fluid is the chitin copper-lactate gray-mold preventing and treating preparation. When used, the chitin copper-lactate gray-mold preventing and treating preparation is diluted by 500 folds to be sprayed to leaves and fruits of plants, active enzymes of pathogens are damaged, and the gray-mold inhibiting effective rate is 93%.

Description

A kind of preparation method of chitin copper lactate gray mold control agent

Technical field

The present invention relates to a kind of preparation method of chitin copper lactate gray mold control agent, belong to the technical field of agro-ecology type control of plant disease.

Background technology

Gray mold is open country, a kind of fungal disease of common and more difficult control in greenhouse gardening, the pathogene of gray mold is that Botrytis cinerea belongs to, happiness grows under the environment of low temperature and high relative humidity, when temperature is at 20-30 DEG C, humidity continues the high-incidence season for disease in the environment of 90%, the flower of plant, really, leaf, stem all can be fallen ill, fruit grows white after infecting, the mould layer of the rear grey having one deck thick, fruit is caused to be the rotten shape of water, gray mold is a kind of typical aeroborne disease, can with air-flow, irrigate or the upper infection of farming operation, infection velocity is fast, as prophylactic treatment infected full garden in 3 days not in time, cause the situation of total crop failure, gray mold is in the control of actual disease, be difficult to take effectively thoroughly to cut off source of infection, when pathogene invades, also be difficult to eliminate pathogen thoroughly, at present under the operation of reality plantation, control for gray mold generally takes fumigation and spraying medicine method two kinds to walk abreast, the medicine used is procymidone, tpn, iprodione, the agricultural chemicals such as the poly-phonetic mould amine of astatine are prevented and treated, although the situation of Pesticide use recited above can be prevented and treated, but its residual pathogene is under suitable environmental condition, breeding still can be continued after drug effect, therefore develop immunity to drugs after pathogene several generations, agricultural chemicals is used to carry out gray mold control, no matter adopt sootiness, the method sprayed, in reality plantation, its pathogene develops immunity to drugs to agricultural chemicals conventional on the market at present, in control more difficulty, and use these agricultural chemicals easily to produce residue of pesticide, land pollution, outside spoiled soil vigor, also the Quality Down of finished product fruit can be caused.

Present consumer payes attention to gradually for nuisanceless, green food, requirement for Detecting Pesticide standard is more and more higher, how a kind of control agent of gray mold of nontoxic, harmless boilogical type is provided, not only can suppress the growth of gray mold, trace element during can providing plant growth again supplements, except not developing immunity to drugs when disease control, the raw material adopted, easily by the organic-biological control agent that soil and plant absorption decompose, is a solution being badly in need of at present wanting on agricultural planting.

Summary of the invention

Technical problem to be solved by this invention is to provide with a kind of antibacterial protein peptide bond chelated nano complexing monovalence copper produced with vegetalitas lactic acid bacteria, forms the strong oxidizer destroying pathogen cells wall, and then the gray mold pathogene that deactivation has been infected, utilize vegetalitas lactic acid bacteria to the function of dissolving chitin, form chitin jelly, in order to the fruit of coated plant, stem, leaf, prevent the effect that pathogene invades, a breathable diaphragm is formed in the appearance of plant, prevent infecting of pathogene, advantage of the present invention not only can not produce pollution by environmental pollution, the composition used is the nutrient of demand of plant growth, easily be absorbed and used by plants, enter in soil and automatically degraded by the microorganism of soil, except being the control agent of the harmless a kind of gray mold of nature, not only play the incidence of disease preventing and treating gray mold, outside the infecting of isolated gray mold, only need in addition to adopt the method sprayed in control, do not need sootiness+spray dual work, reduce and preventing and treating the human input of gray mold, more can increase finished product fruit sugariness and mouthfeel, increase the quality of finished product fruit, the present invention is the biocontrol agent that not only can become economic worth, the otherwise address technical barrier of residue of pesticide and pathogene disease resistance.

The present invention solves the problems of the technologies described above the preparation method that the technical scheme adopted is a kind of chitin copper lactate gray mold control agent, its material mixture ratio and processing step as follows:

1, material:

(1), material I vegetalitas lactobacillus suspension 80%, be main material by sponge gourd tender leaf, fermentation culture is mixed by brown sugar 15%+ zinc sulphate 2%+ manganese sulphate 1.5%+ magnesium sulfate 2%;

(2), material II complexing nano monovalence copper 5%, material III chitin 15%;

2, processing step:

(1), collect sponge gourd tender leaf after, with the ratio of fermentation culture 2:1, put into fermentation cauldron, fermentation 7d, the temperature maintaining fermentation cauldron between yeast phase is between 30 DEG C-35 DEG C, and the rear filtration residue that fermented obtains suspension, is vegetalitas lactobacillus suspension;

(2) that, is blown slowly by material II puts in the vegetalitas lactobacillus suspension of completing steps 1, until zymotic fluid becomes dark green;

(3), further by material III, put into the solution after completing steps 2, after stirring, after leaving standstill 24h, solution becomes viscous fluid, is a kind of chitin copper lactate gray mold control agent;

Advantage of the present invention:

The present invention contrasts prior art following effect: the present invention is on a kind of application sponge gourd blade on every square centimeter, saccharomycete containing 10-15 ten thousand, lactic acid bacteria, culture fluid is utilized to turn out natural vegetalitas lactic acid bacteria and saccharomycete, produce antibacterial protein peptide, copper lactate is formed by antibacterial protein peptide bond chelated nano complexing monovalence copper, the function that chitin forms colloid substance can be dissolved by zymotic fluid, the chitin of powdery is formed colloid, when spraying on plant, not only play the cell wall destroying gray mold pathogene, block the sporulation of pathogene, in addition the fruit of plant, stem, leaf one-tenth one deck has the diaphragm of gas permeability, block the infection way of pathogene, and do not affect the effect of plant photosynthesis and breathing, the fruit plant is directly sprayed during use, stem, Ye Shang, play outside anti-two functions with controlling, element used is in addition the trace element needed for plant, not only can increase outside the nutritional supplementation of plant, can also by the microbial decomposition in soil, there is no the problem of residue of pesticide and soil pollution, the governance role of the present invention to pathogene is by external damage cell wall in addition, not utilize nerve or stomach toxicity sterilized principle for germ, so the pesticide resistance of pathogene can not be caused, it is a kind of product of worth large-scale promotion application, solve the problem that plantation at present relies on agricultural chemicals.

Accompanying drawing explanation

Fig. 1 is the process flow diagram of a kind of preparation method of chitin copper lactate gray mold control agent

Embodiment

1, case one: farm, Zhangjiagang, Suzhou City of Jiangsu Province, utilizes facility plastic greenhouse to plant edible-type tomato 100 mu of ground, the mid-April in this year, the fruit finding that there is the tomato of 30% has the mould layer of grey, through being judged as tomato gray mold; Apply chitin copper lactate gray mold control agent of the present invention 500 times of liquid to spray, within second day, find that mould layer presents and tighten dry shape, through sampling microscopic examination, discovery conidiophore reduces, through laboratory cultures, lose the bacterium colony of gray mold pathogene, after spray 1000 times of liquid, to the symptom having no gray mold when uprooting plants after their edible portions have been harvested every maintenance in 7 days;

2, case two: strawberry cultivating family, Dongshan Town, Suzhou City of Jiangsu Province, application simple plastic greenhouse plantation beauty strawberry 10 booths, in the February in this year, find when temperature raises that the strawberry in booth grows the mould layer of grey, through being judged as grey mould fruit rot of strawberry; Apply chitin copper lactate gray mold control agent of the present invention 500 times of liquid to spray, within second day, find that mould layer presents and tighten dry shape, 1000 times of liquid are sprayed every maintenance in 10 days backward, to the symptom having no gray mold at the end of strawberry at the beginning of 5 months, the sugariness of strawberry fruit is detected as 12-14 degree through sugariness detector, increase 3-4 degree than strawberry sugariness 9-10 originally, arround the strawberry sugariness of plantation family plantation at 7-8 degree;

3, case three: Zhangjiagang, Suzhou City of Jiangsu Province three Fengzhen, apply outdoor plantation summer black grape, when May, grape was born fruit, find that grape occurs white dot and with the situation of the rotten shape of yellow water, be judged as the omen infecting grape grey mould, apply chitin copper lactate gray mold control agent of the present invention 500 times of liquid to spray, within 3 days, check to have no afterwards and spread, sprayed 1000 times of liquid to prevent and treat as maintenance every 15 days, gray mold outbreak is had no when July gathers, its grape painted than arround fast 10 days of vineyard, the residue of pesticide of grape detect in local agriculture test department, do not detect residue of pesticide,

By above actual in the application in land for growing field crops, when between the gray mold high-incidence season, apply a kind of chitin copper lactate gray mold control agent of the present invention 500 times of liquid to spray, treatment infected plant has served the effect of the activity suppressing germ, and spray the protective liquid of low solubility at a fixed time, also can play outside preventive effect, separately can increase colourity and the sugariness of fruit, increasing quality and the price of commodity fruit, is the solution of a kind of green non-poisonous gray mold control be worthy to be popularized.

Although the present invention discloses as above with preferred embodiment; so itself and be not used to limit the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; when doing a little amendment and perfect, therefore protection scope of the present invention is when being as the criterion of defining with claims.

Claims

1. the preparation method of a chitin copper lactate gray mold control agent, it is characterized in that by vegetalitas lactobacillus suspension 80%, complexing nano monovalence copper 5%, chitin 15%, through the Biological Control of Gray Mold agent of a kind of biotype nontoxic residue-free that fermentation is made with the technique of chelating.

2. the preparation method of a kind of chitin copper lactate gray mold control agent as claimed in claim 1, adopts following processing step to make:

1), collect the tender leaf of sponge gourd, with the mixed liquor of brown sugar 15%+ zinc sulphate 2%+ manganese sulphate 1.5%+ magnesium sulfate 2%, carry out fermentation 7d with the ratio of 2:1, the rear filtration residue that fermented obtains suspension, is vegetalitas lactobacillus suspension;

2), further by complexing nano monovalence copper liquid, that blows slowly pours in vegetalitas lactobacillus suspension, until the color of bacterium liquid becomes dark green;

3), further by chitin put in the solution after completing steps 2, after stirring, after leaving standstill 24h, until liquid become thick after namely obtain a kind of chitin copper lactate gray mold control agent.