CN105123527A - Culture bottle provided with built-in fixing frame and applicable to liquid culture medium - Google Patents
Culture bottle provided with built-in fixing frame and applicable to liquid culture medium Download PDFInfo
- Publication number
- CN105123527A CN105123527A CN201510594443.2A CN201510594443A CN105123527A CN 105123527 A CN105123527 A CN 105123527A CN 201510594443 A CN201510594443 A CN 201510594443A CN 105123527 A CN105123527 A CN 105123527A
- Authority
- CN
- China
- Prior art keywords
- bottle
- beam barrel
- applicable
- nutrient medium
- liquid nutrient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A culture bottle provided with a built-in fixing frame and applicable to a liquid culture medium comprises a bottle body and a bottle cover buckling at a bottle mouth of the bottle body and further comprises an air exchange device for realizing air exchange between air in the bottle body and the liquid culture medium, wherein the air exchange device comprises a hollow connecting shaft barrel; the connecting shaft barrel penetrates through the bottom end of the bottle cover to extend into the bottle body; an air exchange core rod matched with the hollow part of the connecting shaft barrel is arranged in the connecting shaft barrel; the air exchange core rod can vertically move along the axis of the connecting shaft barrel to realize air exchange; the bottom end of the connecting shaft barrel is fixedly connected with the fixing device used for fixing plant materials. According to the culture bottle provided by the invention, the fixing device suitable for the liquid culture medium is arranged in the culture bottle, so that effective fixing of plant tissue is realized, liquid culture of plant tissue is realized, demands of plant tissue for oxygen are met, and further requirements of the plant tissue for rapidly and stably growing in the liquid culture medium are met. Air exchange of the culture medium in the culture bottle is realized through the air exchange device.
Description
Technical field
The present invention relates to experiment appliance technical field, be specially adapted to a kind of blake bottle with built-in fixed mount being applicable to liquid nutrient medium.
Background technology
At present, mostly use solid culture medium or semisolid culturemedium in Plant Tissue Breeding, two kinds of medium are all based on liquid nutrient medium, and the agar adding different proportion is formulated.Solid culture medium and semisolid culturemedium have certain supporting role to plant tissue, but due to less with the contact surface of plant tissue, nutrient absorption is slow, causes tissue growth delayed.Liquid nutrient medium compares first two medium, and to have quality homogeneous, contact surface is large, can promote the advantages such as plant tissue ramp, but lacks supporting role to plant tissue due to liquid nutrient medium, and easily anaerobic environment is caused to culture, thus fail extensive use.
Summary of the invention
For overcoming problems of the prior art, the invention provides a kind of can effectively fixing plant tissue and the blake bottle of amount of oxygen abundance.
The present invention solves the technical scheme that its technical problem takes: this kind is applicable to the blake bottle with built-in fixed mount of liquid nutrient medium, comprise bottle and buckle the bottle cap at bottle body and bottleneck place, it is characterized in that: also comprise the air interchanger realizing taking a breath between air and liquid nutrient medium in bottle, air interchanger comprises the connection beam barrel of hollow, connecting beam barrel stretches in bottle through bottle cap bottom, and the top connecting beam barrel is uncovered and be positioned at outside bottle; In connection beam barrel, be provided with the ventilation core bar coordinated with connecting axle hollow space, outside the top of ventilation core bar, be exposed at bottle outward and be fixedly connected with handle; Ventilation core bar can move up and down along connection beam barrel axis; The bottom of connection beam barrel stretches into below liquid nutrient medium liquid level and there is the air-vent being communicated with and connecting beam barrel and bottle bottom, and the bottom connecting beam barrel is fixedly connected with the fixture of fixing vegetable material, fixture comprises circular fixed head, and fixed head is provided with some fixing holes for fixing vegetable material; Be provided with supporting leg in the bottom of fixed head, supporting leg is supported on position at the bottom of bottle.
Further, described bottle cap is provided with ventilative filter paper.
Further, described fixture be connected beam barrel and be stainless steel.
Further, the hollow space of described connection beam barrel is cross section is circular cavity, and ventilation core bar is high temperature resistant rigid plastics material, and is equipped with resistant to elevated temperatures rubber sleeve in the bottom of ventilation core bar.
Further, the hollow space of described connection beam barrel to be cross section be circular cavity, ventilation core bar is stainless steel and the smooth cylinder in its bottom, and the diameter of cylinder is identical with the diameter of cavity, and cylinder and cavity inner wall fit tightly.
Further, the fixing hole on described fixed head is square opening and/or circular port.
Further, described supporting leg has three, and three supporting leg is isometric and supporting leg is uniform at the marginal position of fixed head.
Further, this fixed head is applicable to stem apex to different vegetable material, stem section, stem tuber, bulb, blade, inflorescence, petal, ovary, flower pesticide, fruit, seed carry out cultivation and fix.
To sum up, the beneficial effect of technique scheme of the present invention is as follows:
The present invention by arranging the fixture being applicable to liquid nutrient medium in blake bottle, the supporting leg of fixture is supported in blake bottle, the fixed head of fixture is supported on liquid nutrient medium ullage, fixed head can realize effectively fixing plant tissue, make to stretch into below medium liquid level bottom plant tissue to be cultivated, top is then fixed on fixed head and directly contacts with aerobic air in blake bottle, both the liquid culture of plant tissue had been met, meet again the demand of plant tissue to oxygen, and then meet in plant tissue liquid medium within quick, the requirement of stable growth.
By connecting beam barrel and ventilation core bar coordinates the air interchanger forming syringe-like structure, ventilation core bar moves up and down the ventilation achieved medium in blake bottle bottle in beam barrel connecting.
Accompanying drawing explanation
Fig. 1 is perspective view of the present invention;
Fig. 2 is front view of the present invention;
Fig. 3 is the partial enlarged drawing (embodiment one) of Fig. 2 part A;
Fig. 4 is the partial enlarged drawing (embodiment two) of Fig. 2 part A;
In figure:
1 bottle, 2 bottle caps, 3 connect beam barrel, 4 ventilation core bars, 4 ' ventilation core bar, 5 rubber sleeves, 6 handles, 7 fixed heads, 8 fixing holes, 9 supporting legs, 10 filter paper, 11 liquid nutrient mediums, 12 plant tissue materials.
Embodiment
Be described in detail to characteristic sum principle of the present invention below in conjunction with accompanying drawing, illustrated embodiment, only for explaining the present invention, not limits protection scope of the present invention with this.
As shown in Figure 1, this invention mainly comprises bottle 1, buckles bottle cap 2 at bottle body and bottleneck place, realizes the air interchanger of taking a breath between air and liquid nutrient medium in bottle and plant tissue fixture.
Wherein, the bottle of blake bottle is cylindric uncovered bottle, is buckled with a bottle cap at the position of bottleneck of bottle.In order to realize the consistent of bottle inner and outer air pressure, bottle cap is provided with a ventilative filter paper 10, and filter paper is arranged on bottle cap non-central location, filter paper, except ensureing that bottle inner and outer air pressure unanimously, also serves the effect of the pollutant such as isolate bacteria, fungi.
The center of bottle cap is for connecting air interchanger, and the center why air interchanger is arranged on bottle cap is to topple over when preventing from ensureing that blake bottle is placed on experimental bench.Described air interchanger comprises and connects beam barrel 3, connects in beam barrel to coordinate to be provided with ventilation core bar 4, connects beam barrel and coordinates with core bar of taking a breath and form the air interchanger identical with conventional syringe operation principle.Connect beam barrel as piston cylinder, ventilation core bar is as piston, and ventilation core bar can freely up and down pull be movable in connection beam barrel.
Connecting beam barrel stretches in bottle through bottle cap bottom, and the top connecting beam barrel is uncovered and be positioned at outside bottle.Be provided with the air-vent being communicated with and connecting beam barrel and bottle connecting the bottom of beam barrel, the bottom of connection beam barrel be extend into liquid nutrient medium 11 in bottle liquid level below, as shown in Figure 2.The bottom of ventilation core bar is stretched into and is connected in beam barrel, and top is then stretched out from the open top end connecting beam barrel and fixed with handle 6, can singlehanded driving handle during use, convenient operation.
Be fixedly connected with in the bottom connecting beam barrel for the fixing fixture of plant tissue materials 12, described fixture comprises circular fixed head 7, and the diameter of fixed head is less than bottle intracavity diameter, ensures that fixed head can be put into, take out from the bottleneck of bottle.Fixed head is provided with some fixing holes for fixing plant tissue materials, and fixing hole 8 runs through fixed head.Be provided with supporting leg 9 in the bottom of fixed head, fixed head is supported on bottle body bottom by supporting leg, and makes on fixed head liquid medium within liquid level.Further, in order to make fixed head to be placed in bottle stably, three isometric supporting legs can be fixed in the bottom of fixed head.Being assigned plant tissue materials to be cultivated is fixed in the fixing hole of fixed head, and be inserted into during the bottom of plant tissue materials in liquid nutrient medium, the top of plant tissue materials is fixed in fixing hole, directly contact with the filtrated air part in bottle, plant tissue materials both can abundant contact liq medium, can normal respiration be carried out again, reach the effect of fast and stable breeding.
In order to ensure the stability of fixture and the easily sterilising of blake bottle entirety, stainless steel is selected to make fixture and connecting axle.
Embodiment one: the hollow space of described connection beam barrel is cross section is circular cavity, ventilation core bar is that resistant to elevated temperatures rigid plastics material makes, and be equipped with high temperature high voltage resistant (pressure 103kPa-137kPa in the bottom of ventilation core bar, temperature 121 DEG C-126 DEG C, time 20min-30min, wherein the rubber sleeve of high temperature high voltage resistant can select material of the prior art to make) rubber sleeve 5, the cavity inner wall of rubber sleeve and connecting axle fits tightly, as shown in Figure 3, along connection beam barrel upper and lower pull ventilation core bar, when ventilation core bar is promoted downwards, ventilation core bar promotes the air in squeeze flow beam barrel, compressed air enters in liquid nutrient medium through the air-vent connecting beam barrel bottom, medium by compression air act on liquid level under produce bubble, realize the ventilation of liquid nutrient medium and filtrated air, filtrated air is changed in liquid nutrient medium, ensure that plant tissue materials immerses the eupnea effect of liquid culture base section.Adopt plastic material to make ventilation core bar and can reduce manufacturing cost, cost is lower.
Embodiment two: the cross section of the hollow space of described connection beam barrel is circular cavity, ventilation core bar 4 ' is for stainless steel and the smooth cylinder in its bottom, and as shown in Figure 4, the diameter of cylinder is identical with the diameter of cavity, and cylinder and cavity inner wall fit tightly.Its operation principle is identical with the principle of glass syringe, and implementation step is identical with embodiment one, does not repeat them here.
Further, the fixing hole on described fixed head is square opening and/or circular port, or the hole of shape that other and plant tissue materials match.
Further, this fixed head stem apex, stem section, stem tuber, bulb, blade, inflorescence, petal, ovary, flower pesticide, fruit, seed etc. be applicable to different vegetable material carry out cultivation fix.Grid minimum aperture can be arranged different size; 0.2cm ╳ 0.2cm, 0.3cm ╳ 0.3cm, 0.4cm ╳ 0.4cm, 0.5cm ╳ 0.5cm, 0.6cm ╳ 0.6cm etc.
On superclean bench, bottle cap is opened, pour liquid nutrient medium into; According to the size of ready organization material (stem apex, stem section, stem tuber, bulb, blade, inflorescence, petal, ovary, flower pesticide, holder, fruit, seed etc.), select the fixed head of different size; With tweezers, sterilizing, the organization material that cuts are put into the fixing hole of the fixed head of hollow out; Fixed head is carefully put into blake bottle, ensure that material lower end fully contacts with liquid nutrient medium, and upper end exceeds horizontal plane, fully carries out respiration; Blake bottle is put into cultivation hot-house culture.2-3 days, for avoiding carrying out anaerobic respiration in the liquid medium within of material lower end and producing material that is poisonous or Developing restraint, blake bottle is put into superclean bench, after carrying out sufficiently sterilised sterilizing outside bottle, promote ventilation core bar up and down, air part in bottle is filled with in liquid nutrient medium, ensures the eupnea effect of plant tissue materials lower end tissue.
The present invention by arranging the fixture being applicable to liquid nutrient medium in blake bottle, the supporting leg of fixture is supported in blake bottle, the fixed head of fixture is supported on liquid nutrient medium ullage, fixed head can realize effectively fixing plant tissue, make to stretch into below medium liquid level bottom plant tissue to be cultivated, top is then fixed on fixed head and directly contacts with aerobic air in blake bottle, both the liquid culture of plant tissue had been met, meet again the demand of plant tissue to oxygen, and then meet in plant tissue liquid medium within quick, the requirement of stable growth.
By connecting beam barrel and ventilation core bar coordinates the air interchanger forming syringe-like structure, ventilation core bar moves up and down the ventilation achieved medium in blake bottle bottle in beam barrel connecting.
Above-described embodiment is only the description carried out the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; relevant technical staff in the field, to various distortion of the present invention and improvement, all should expand in the determined protection domain of claims of the present invention.
Claims (8)
1. one kind is applicable to the blake bottle with built-in fixed mount of liquid nutrient medium, comprise bottle and buckle the bottle cap at bottle body and bottleneck place, it is characterized in that: also comprise the air interchanger realizing taking a breath between air and liquid nutrient medium in bottle, air interchanger comprises the connection beam barrel of hollow, connecting beam barrel stretches in bottle through bottle cap bottom, and the top connecting beam barrel is uncovered and be positioned at outside bottle; In connection beam barrel, be provided with the ventilation core bar coordinated with connecting axle hollow space, outside the top of ventilation core bar, be exposed at bottle outward and be fixedly connected with handle; Ventilation core bar can move up and down along connection beam barrel axis; The bottom of connection beam barrel stretches into below liquid nutrient medium liquid level and there is the air-vent being communicated with and connecting beam barrel and bottle bottom, and the bottom connecting beam barrel is fixedly connected with the fixture of fixing plant tissue materials, fixture comprises circular fixed head, and fixed head is provided with some fixing holes for fixing plant tissue materials; Be provided with supporting leg in the bottom of fixed head, supporting leg is supported on position at the bottom of bottle.
2. a kind of blake bottle with built-in fixed mount being applicable to liquid nutrient medium according to claim 1, is characterized in that: described bottle cap is provided with ventilative filter paper.
3. a kind of blake bottle with built-in fixed mount being applicable to liquid nutrient medium according to claim 1, is characterized in that: described fixture be connected beam barrel and be stainless steel.
4. a kind of blake bottle with built-in fixed mount being applicable to liquid nutrient medium according to claim 1, it is characterized in that: the hollow space of described connection beam barrel is cross section is circular cavity, ventilation core bar is high temperature resistant rigid plastics material, and is equipped with the rubber sleeve of high temperature high voltage resistant in the bottom of ventilation core bar.
5. a kind of blake bottle with built-in fixed mount being applicable to liquid nutrient medium according to claim 1, it is characterized in that: the hollow space of described connection beam barrel is cross section is circular cavity, ventilation core bar is stainless steel and its bottom is smooth cylinder, the diameter of cylinder is identical with the diameter of cavity, and cylinder and cavity inner wall fit tightly.
6. a kind of blake bottle with built-in fixed mount being applicable to liquid nutrient medium according to claim 1, is characterized in that: the fixing hole on described fixed head is square opening and/or circular port.
7. a kind of blake bottle with built-in fixed mount being applicable to liquid nutrient medium according to claim 1, is characterized in that: described supporting leg has three, and three supporting leg is isometric and supporting leg is uniform at the marginal position of fixed head.
8. a kind of blake bottle with built-in fixed mount being applicable to liquid nutrient medium according to claim 1, is characterized in that: this fixed head is applicable to stem apex to different plant tissue materials, stem section, stem tuber, bulb, blade, inflorescence, petal, ovary, flower pesticide, fruit, seed carry out cultivation and fix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510594443.2A CN105123527A (en) | 2015-09-17 | 2015-09-17 | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510594443.2A CN105123527A (en) | 2015-09-17 | 2015-09-17 | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105123527A true CN105123527A (en) | 2015-12-09 |
Family
ID=54709424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510594443.2A Pending CN105123527A (en) | 2015-09-17 | 2015-09-17 | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105123527A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113598058A (en) * | 2021-09-13 | 2021-11-05 | 广西壮族自治区农业科学院 | Improved method for sugarcane stem tip culture |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0856250B1 (en) * | 1995-10-30 | 2001-02-21 | Kubota Corporation | Plant culture vessel |
| CN201284337Y (en) * | 2008-10-31 | 2009-08-05 | 吉林农业大学 | Culture bottle with ventilating device |
| CN201947752U (en) * | 2010-11-22 | 2011-08-31 | 北方民族大学 | Tissue culture bottle for liquid culturing of potato detoxification base seedlings |
| CN103493732A (en) * | 2013-09-18 | 2014-01-08 | 天津海友佳音生物科技股份有限公司 | Temperature-controllable large-size alga suspension culture device with spore adhesion components |
| CN205005668U (en) * | 2015-09-17 | 2016-02-03 | 山东省农作物种质资源中心 | Blake bottle that has built -in mount suitable for liquid medium |
-
2015
- 2015-09-17 CN CN201510594443.2A patent/CN105123527A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0856250B1 (en) * | 1995-10-30 | 2001-02-21 | Kubota Corporation | Plant culture vessel |
| CN201284337Y (en) * | 2008-10-31 | 2009-08-05 | 吉林农业大学 | Culture bottle with ventilating device |
| CN201947752U (en) * | 2010-11-22 | 2011-08-31 | 北方民族大学 | Tissue culture bottle for liquid culturing of potato detoxification base seedlings |
| CN103493732A (en) * | 2013-09-18 | 2014-01-08 | 天津海友佳音生物科技股份有限公司 | Temperature-controllable large-size alga suspension culture device with spore adhesion components |
| CN205005668U (en) * | 2015-09-17 | 2016-02-03 | 山东省农作物种质资源中心 | Blake bottle that has built -in mount suitable for liquid medium |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113598058A (en) * | 2021-09-13 | 2021-11-05 | 广西壮族自治区农业科学院 | Improved method for sugarcane stem tip culture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103918418B (en) | The storage device of Seeds of Dendrobium Candidum and method for preserving thereof | |
| CN204317213U (en) | A kind of processing tomato hydroponic device | |
| CN205005668U (en) | Blake bottle that has built -in mount suitable for liquid medium | |
| CN203399582U (en) | Chinese chive water planting device | |
| CN105123527A (en) | Culture bottle provided with built-in fixing frame and applicable to liquid culture medium | |
| CN102511451B (en) | Insect breeding cage | |
| CN206776152U (en) | A kind of plant disease inoculation case | |
| CN206760183U (en) | A kind of succulent planting unit | |
| CN206629669U (en) | Water planting takes root device and blueberry water planting is taken root device | |
| CN206314442U (en) | A kind of diamondback moth larvae and the breeding apparatus of adult | |
| CN105660343A (en) | Soil-less culture tray for allium ascalonicum | |
| CN105210866B (en) | A kind of intermittent immersed orchid protocorms propagation quick-breeding method | |
| CN208191731U (en) | Japanese Premna tissue culture flasks | |
| CN202340622U (en) | Insect breeding cage | |
| CN105191798B (en) | A kind of intermittent immersed lily bud scale propagation quick-breeding method | |
| CN105340733B (en) | A kind of intermittent immersed Conandron ramondioides sieb. Et zuce blade adventitious bud inducing method | |
| CN203340739U (en) | Indoor movable multifunctional soilless culture device | |
| CA3007538A1 (en) | Bioreactor | |
| CN208387643U (en) | A kind of adjustable quantitative fish feeding device | |
| CN209518052U (en) | A kind of tiny seed disinfection case efficiently, easy | |
| CN203788757U (en) | Preserving device for dendrobium officinale seeds | |
| JP2014060930A (en) | Hydroponics apparatus | |
| CN207978516U (en) | A kind of lily breeding apparatus | |
| TWI417381B (en) | Cactus camellia fruiting body and mycelia fast culture flask device | |
| CN105766455A (en) | Sterile and energy-saving experimental apparatus for plant seedling raising |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151209 |
|
| WD01 | Invention patent application deemed withdrawn after publication |