CN105112367A - 一种间充质干细胞表皮分化诱导剂及其应用方法 - Google Patents
一种间充质干细胞表皮分化诱导剂及其应用方法 Download PDFInfo
- Publication number
- CN105112367A CN105112367A CN201510538443.0A CN201510538443A CN105112367A CN 105112367 A CN105112367 A CN 105112367A CN 201510538443 A CN201510538443 A CN 201510538443A CN 105112367 A CN105112367 A CN 105112367A
- Authority
- CN
- China
- Prior art keywords
- stem cell
- cell
- bmp
- epidermal differentiation
- mesenchymal stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000004069 differentiation Effects 0.000 title claims abstract description 37
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title claims description 12
- 239000000411 inducer Substances 0.000 title abstract 4
- 210000000130 stem cell Anatomy 0.000 claims abstract description 27
- OHCQJHSOBUTRHG-KGGHGJDLSA-N FORSKOLIN Chemical compound O=C([C@@]12O)C[C@](C)(C=C)O[C@]1(C)[C@@H](OC(=O)C)[C@@H](O)[C@@H]1[C@]2(C)[C@@H](O)CCC1(C)C OHCQJHSOBUTRHG-KGGHGJDLSA-N 0.000 claims abstract description 20
- SUZLHDUTVMZSEV-UHFFFAOYSA-N Deoxycoleonol Natural products C12C(=O)CC(C)(C=C)OC2(C)C(OC(=O)C)C(O)C2C1(C)C(O)CCC2(C)C SUZLHDUTVMZSEV-UHFFFAOYSA-N 0.000 claims abstract description 10
- 108010002350 Interleukin-2 Proteins 0.000 claims abstract description 10
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims abstract description 10
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims abstract description 10
- OHCQJHSOBUTRHG-UHFFFAOYSA-N colforsin Natural products OC12C(=O)CC(C)(C=C)OC1(C)C(OC(=O)C)C(O)C1C2(C)C(O)CCC1(C)C OHCQJHSOBUTRHG-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000000694 effects Effects 0.000 claims abstract description 10
- 108010000685 platelet-derived growth factor AB Proteins 0.000 claims abstract description 10
- 108010049955 Bone Morphogenetic Protein 4 Proteins 0.000 claims abstract description 9
- 102100024505 Bone morphogenetic protein 4 Human genes 0.000 claims abstract description 9
- 108090000385 Fibroblast growth factor 7 Proteins 0.000 claims abstract description 7
- 239000002609 medium Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 6
- 239000012679 serum free medium Substances 0.000 claims description 6
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 abstract description 46
- 230000008859 change Effects 0.000 abstract description 5
- 108090000695 Cytokines Proteins 0.000 abstract description 3
- 102000004127 Cytokines Human genes 0.000 abstract description 3
- 206010028980 Neoplasm Diseases 0.000 abstract description 2
- 201000011510 cancer Diseases 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract description 2
- 238000001890 transfection Methods 0.000 abstract description 2
- 210000001339 epidermal cell Anatomy 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 14
- 230000001939 inductive effect Effects 0.000 description 12
- 108010022222 Integrin beta1 Proteins 0.000 description 10
- 102000012355 Integrin beta1 Human genes 0.000 description 10
- 230000006698 induction Effects 0.000 description 10
- 210000003954 umbilical cord Anatomy 0.000 description 10
- 210000003491 skin Anatomy 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 210000004204 blood vessel Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 3
- 101000998011 Homo sapiens Keratin, type I cytoskeletal 19 Proteins 0.000 description 3
- 102100033420 Keratin, type I cytoskeletal 19 Human genes 0.000 description 3
- 108060000200 adenylate cyclase Proteins 0.000 description 3
- 102000030621 adenylate cyclase Human genes 0.000 description 3
- 230000001079 digestive effect Effects 0.000 description 3
- 210000002514 epidermal stem cell Anatomy 0.000 description 3
- 230000008175 fetal development Effects 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 102100022464 5'-nucleotidase Human genes 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 2
- 102000006354 HLA-DR Antigens Human genes 0.000 description 2
- 108010058597 HLA-DR Antigens Proteins 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- 101000678236 Homo sapiens 5'-nucleotidase Proteins 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 description 2
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 2
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 2
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 description 2
- 102100025304 Integrin beta-1 Human genes 0.000 description 2
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 2
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 2
- 210000001691 amnion Anatomy 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 235000011089 carbon dioxide Nutrition 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000002458 cell surface marker Substances 0.000 description 2
- 230000009194 climbing Effects 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 239000003636 conditioned culture medium Substances 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000004130 lipolysis Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 230000001172 regenerating effect Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 229930002330 retinoic acid Natural products 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000012422 test repetition Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- JYGRAOYMDDFOSM-FQJIPJFPSA-N (4s)-4-[[(2s)-4-carboxy-2-[[(2s)-3-carboxy-2-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]propanoyl]amino]butanoyl]amino]-5-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-hydroxy-1-oxopropan-2-yl]amino]-5-oxopentano Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN JYGRAOYMDDFOSM-FQJIPJFPSA-N 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102100037241 Endoglin Human genes 0.000 description 1
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 1
- 230000010558 Gene Alterations Effects 0.000 description 1
- 101000881679 Homo sapiens Endoglin Proteins 0.000 description 1
- 101000994375 Homo sapiens Integrin alpha-4 Proteins 0.000 description 1
- 102100032818 Integrin alpha-4 Human genes 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 208000028990 Skin injury Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 description 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000000080 chela (arthropods) Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000001612 chondrocyte Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000024835 cytogamy Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 108010053500 delicious peptide Proteins 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000007443 liposuction Methods 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000003716 mesoderm Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000008215 water for injection Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510538443.0A CN105112367B (zh) | 2015-08-28 | 2015-08-28 | 一种间充质干细胞表皮分化诱导剂及其应用方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510538443.0A CN105112367B (zh) | 2015-08-28 | 2015-08-28 | 一种间充质干细胞表皮分化诱导剂及其应用方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105112367A true CN105112367A (zh) | 2015-12-02 |
CN105112367B CN105112367B (zh) | 2018-07-27 |
Family
ID=54660482
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510538443.0A Active CN105112367B (zh) | 2015-08-28 | 2015-08-28 | 一种间充质干细胞表皮分化诱导剂及其应用方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105112367B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111454896A (zh) * | 2020-04-10 | 2020-07-28 | 南京大学 | 一种提高间充质干细胞促进急性髓系白血病细胞分化能力的诱导方法 |
CN114438025A (zh) * | 2022-02-22 | 2022-05-06 | 辛志远 | 一种制备脂肪间充质干细胞因子的诱导培养基及培养方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003018760A2 (en) * | 2001-08-24 | 2003-03-06 | Advanced Cell Technology, Inc. | Screening assays for identifying differentiation-inducing agents and production of differentiated cells for cell therapy |
CN1871343A (zh) * | 2003-11-04 | 2006-11-29 | 株式会社宝玛斯特 | 从脂肪组织制备干细胞的方法和*** |
WO2007075807A2 (en) * | 2005-12-20 | 2007-07-05 | Es Cell International Pte Ltd. | Methods for the directed differentiation of embryonic stem cell |
CN102433302A (zh) * | 2011-12-15 | 2012-05-02 | 成都清科生物科技有限公司 | 一种间充质干细胞无血清培养基 |
CN104388381A (zh) * | 2014-10-31 | 2015-03-04 | 浙江大学 | 一种人骨髓间充质干细胞诱导分化内耳毛细胞的方法 |
CN104762259A (zh) * | 2015-04-21 | 2015-07-08 | 广州赛莱拉干细胞科技股份有限公司 | 一种间充质干细胞的培养基及其大规模培养方法 |
-
2015
- 2015-08-28 CN CN201510538443.0A patent/CN105112367B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003018760A2 (en) * | 2001-08-24 | 2003-03-06 | Advanced Cell Technology, Inc. | Screening assays for identifying differentiation-inducing agents and production of differentiated cells for cell therapy |
CN1871343A (zh) * | 2003-11-04 | 2006-11-29 | 株式会社宝玛斯特 | 从脂肪组织制备干细胞的方法和*** |
WO2007075807A2 (en) * | 2005-12-20 | 2007-07-05 | Es Cell International Pte Ltd. | Methods for the directed differentiation of embryonic stem cell |
CN102433302A (zh) * | 2011-12-15 | 2012-05-02 | 成都清科生物科技有限公司 | 一种间充质干细胞无血清培养基 |
CN104388381A (zh) * | 2014-10-31 | 2015-03-04 | 浙江大学 | 一种人骨髓间充质干细胞诱导分化内耳毛细胞的方法 |
CN104762259A (zh) * | 2015-04-21 | 2015-07-08 | 广州赛莱拉干细胞科技股份有限公司 | 一种间充质干细胞的培养基及其大规模培养方法 |
Non-Patent Citations (7)
Title |
---|
万振洲等: "体外诱导胎盘间充质干细胞向表皮细胞的分化", 《中国组织工程研究与临床康复》 * |
何丽娟等: "自体骨髓间充质干细胞为种子细胞构建组织工程化全层皮肤", 《中国科学》 * |
张景云: "《实用美容药物学》", 31 July 2006, 中国中医药出版社 * |
李仙松等: "大鼠骨髓间充质干细胞重建表皮细胞及皮肤附件", 《中国组织工程研究》 * |
李平风等: "Forskolin对成骨样细胞蛋白激酶c及三磷酸肌醇的影响", 《生物化学杂志》 * |
许伟石等: "《烧伤创面修复》", 31 October 2013, 湖北科学技术出版社 * |
韩桂林等: "体外诱导犬骨髓间充质干细胞向上皮样细胞分化的初步研究", 《第二军医大学学报》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111454896A (zh) * | 2020-04-10 | 2020-07-28 | 南京大学 | 一种提高间充质干细胞促进急性髓系白血病细胞分化能力的诱导方法 |
CN111454896B (zh) * | 2020-04-10 | 2023-02-03 | 南京大学 | 一种提高间充质干细胞促进急性髓系白血病分化能力的诱导方法 |
CN114438025A (zh) * | 2022-02-22 | 2022-05-06 | 辛志远 | 一种制备脂肪间充质干细胞因子的诱导培养基及培养方法 |
CN114438025B (zh) * | 2022-02-22 | 2024-01-12 | 深圳市华晨阳科技有限公司 | 一种制备脂肪间充质干细胞因子的诱导培养基及培养方法 |
Also Published As
Publication number | Publication date |
---|---|
CN105112367B (zh) | 2018-07-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112322580B (zh) | 一种间充质干细胞无血清培养基的应用 | |
CN101748096B (zh) | 亚全能干细胞、其制备方法及其用途 | |
Cheng et al. | The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities | |
CN103266081B (zh) | 从脐带中分离培养间充质干细胞的高效方法 | |
CN109234229B (zh) | 从胎盘血管分离间充质干细胞的方法和所用消化酶组合物 | |
KR20070104735A (ko) | 중간엽 줄기세포 배양 배지 및 이를 이용한 중간엽줄기세포의 배양 방법 | |
CN108300690A (zh) | 一种脂肪间充质干细胞的分离培养方法和无血清培养基 | |
CN104762257B (zh) | 一种从脐带制备间充质干细胞的方法 | |
CN104099294A (zh) | 基于干细胞分泌因子的培养基及其制备和使用方法 | |
CN104726406A (zh) | 一种诱导牙髓间充质干细胞分化为神经细胞的方法 | |
CN104651305A (zh) | 一种利用脐带间充质干细胞获取生物活性蛋白的方法 | |
CN102028970A (zh) | 一种用于治疗肝硬化的干细胞制剂 | |
CN106929470A (zh) | 一种用于间充质干细胞体外培养和扩增的无血清培养基 | |
CN101821383A (zh) | 一种用于人成体原始间充质干细胞体外规模化培养的培养基、方法及获得的原始间充质干细胞及其应用 | |
CN107267453A (zh) | 一种用于培养脂肪间充质干细胞的培养基及其应用 | |
CN104707140A (zh) | 治疗骨关节炎的组合物 | |
CN108823148A (zh) | 一种脂肪间充质干细胞诱导分化为肝脏样细胞的方法 | |
CN106957818A (zh) | 一种高效促使脂肪干细胞增殖的方法及其试剂盒 | |
CN106566803A (zh) | 一种培养液及其应用和培养脐带间充质干细胞的方法 | |
CN107287156A (zh) | 一种脂肪间充质干细胞的分离培养方法及其应用 | |
CN103215222A (zh) | 一种将人脂肪间充质干细胞诱导成神经细胞的诱导培养基及方法 | |
CN105062970A (zh) | 一种将间充质干细胞诱导成神经细胞的诱导剂及诱导分化完全培养基 | |
CN107083359A (zh) | 干细胞培养基及干细胞分离方法 | |
CN105112367A (zh) | 一种间充质干细胞表皮分化诱导剂及其应用方法 | |
CN111849878A (zh) | 一种提高间充质干细胞成骨能力的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20160824 Address after: 266109, No. 368, Hedong Road, hi tech Zone, Shandong, Qingdao, Qingdao 101, room 7, blue bio Pharmaceutical Industrial Park Applicant after: QINGDAO RUISIKE BIOTECHNOLOGY CO.,LTD. Address before: 101200 Pinggu City, North Street, Beijing District, building No. 13, room No. 802 Applicant before: BEIJING RE-STORE BIOMEDICINE RESEARCH INSTITUTE |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20181108 Address after: 266109 1st and 2nd floors, 7th Building, Incubation Center of Qingdao Blue Biomedical Industrial Park, 368 Hedong Road, Qingdao Hi-tech District, Qingdao City, Shandong Province Patentee after: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Address before: 266109 Qingdao No. 368 Hedong Road, Qingdao high tech Zone, Shandong, 101, room 7, blue biomedical industry park. Patentee before: QINGDAO RUISIKE BIOTECHNOLOGY CO.,LTD. |
|
TR01 | Transfer of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Mesenchymal stem cell epidermal differentiation inducer and its application method Effective date of registration: 20220325 Granted publication date: 20180727 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370010040 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230328 Granted publication date: 20180727 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370010040 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A mesenchymal stem cell epidermal differentiation inducer and its application method Effective date of registration: 20230329 Granted publication date: 20180727 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023370010033 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20180727 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023370010033 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A mesenchymal stem cell epidermal differentiation inducer and its application method Granted publication date: 20180727 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024370010043 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |