CN105067772A - Method used for determining plant total photosynthesis carbon assimilation capacity - Google Patents
Method used for determining plant total photosynthesis carbon assimilation capacity Download PDFInfo
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Abstract
The invention discloses a method used for determining plant total photosynthesis carbon assimilation capacity. According to the method, double-way isotope labeling and a double source isotope mixed module are adopted to determine using ratio of plant on added bicarbonate ions and using ratio of plant on bicarbonate ions come from atmospheric carbon dioxide dissolving; a photosynthesis device is adopted to determine photosynthesis assimilation capacity of plant on atmospheric carbon dioxide; and plant total photosynthesis carbon assimilation capacity is obtained finally. The method is used for measuring using capacity of plant on added bicarbonate ions, and measuring using capacity of plant on bicarbonate ions come from atmospheric carbon dioxide dissolving; and influences of old carbon on determination results are avoided; so that obtained plant total photosynthesis carbon assimilation capacity data is reliable, and result accuracy is high.
Description
Technical field
The present invention relates to a kind of method measuring plant gross photosynthesis carbon assimilation ability, belong to Eco-Environment System monitoring, administer and reparation field.
Background technology
In recent years, numerous experiments is verified, and plant can not only utilize the carbon dioxide in air to carry out photosynthesis as substrate, and can utilize and come from the bicarbonate ion being stored in blade in soil for substrate and carry out photosynthesis.Especially in the karst In Limestone Area of bicarbonate ion with high concentration, only by the inorganic carbon assimilative capacity measuring plant based on the photosynthetic instrument measuring atmospheric carbon dioxide flux, the yield-power of karst plant is seriously underestimated.In view of the Utilization ability of plant to bicarbonate ion can not be ignored, therefore, Accurate Determining plant is to the equipment usage of the inorganic carbon source of different external source, measure the gross photosynthesis carbon assimilation ability comprising carbon dioxide and bicarbonate ion assimilation, to the yield-power of correct assessment plant, the karst adaptive plant kind of screening high productivity, administers with karst adaptive plant and recovers fragile Karst ecosystem and have important effect.
Stable carbon isotope technique has successfully been applied to the fields such as ecological, the two-way carbon isotope labelling method of Utilization ability of the bicarbonate ion of current mensuration plant, can obtain the equipment usage of plant to the bicarbonate ion that external source is added by the bicarbonate ion adding carbon isotope two-way mark in nutrient solution.But the carbon dioxide in air, also can be dissolved in nutrient solution and generate bicarbonate ion, this part bicarbonate ion also can be utilized by plant.The total bicarbonate ion equipment usage of plant is plant to the equipment usage of the bicarbonate ion that external source is added and plant to from atmospheric carbon dioxide lysigenous bicarbonate ion equipment usage sum.And yet there are no and be plant to the quantitative report from atmospheric carbon dioxide lysigenous bicarbonate ion equipment usage.Plant gross photosynthesis carbon assimilation ability comprises plant to the assimilative capacity of atmospheric carbon dioxide and plant to the assimilative capacity of the bicarbonate ion from root absorption.In view of the complicacy that the bicarbonate ion equipment usage that plant is total measures, cause and there is no a kind of method at present to measure plant gross photosynthesis carbon assimilation ability.The present invention then discloses the method for a set of mensuration plant gross photosynthesis carbon assimilation ability, to overcome many disadvantages and the defect of previous method.
Summary of the invention
The technical problem to be solved in the present invention is, a kind of method measuring plant total carbon assimilative capacity is provided, with overcome in prior art lack plant on from the lysigenous bicarbonate ion equipment usage of atmospheric carbon dioxide quantitatively and do not consider that old " carbon " is on the deficiency of the impact of measurement result, to solve in prior art a mensuration difficult problem for substantially understate yield-power because of non-Accurate Measurement plant carbon assimilative capacity.
The present invention takes following technical scheme: it comprises the following steps: the first, and the cave of indoor employing same specification dish water planting sprouts vegetable seeds, and preparation nutrient solution grows seedlings to more than 2 months, and the more consistent seedling of growth selection is as investigation plant seedlings; The second, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13c value difference value is greater than the sodium bicarbonate of 10 ‰ as isotope labeling 1 and isotope labeling 2, and its value is respectively δ
c1and δ
c2, add to respectively in nutrient solution; The δ of bicarbonate ion in the nutrient solution of isotope labeling 1
13c value is δ
c1,0, the δ of bicarbonate ion in isotope labeling 2 nutrient solution
13c value is δ
c2,0; 3rd, the plant seedlings at use the nutrient solution of isotope labeling 1 and isotope labeling 2 respectively more than 2 months monthly ages that incubation growth is consistent simultaneously, the corresponding nutrient solution more renewed every day; 4th, cultivate and start namely to cultivate the leaf area LA that the 0th day measures investigated plant first expanded leaves
0; Before renewing nutrient solution after 1 day, the stable carbon isotope composition δ of the nutrient solution that mensuration two kinds is isotope-labeled, corresponding respectively
13the value of C, δ
nS1and δ
nS2value; 5th, cultivate the leaf area LA measuring this first expanded leaves of investigated plant for 7 days afterwards
7, subsequently, measure the stable carbon isotope composition δ of this first expanded leaves of the plant that two kinds of isotope-labeled nutrient solutions are cultivated respectively
13the value of C, δ
t1and δ
t2; 6th, respectively by δ
c1, δ
c2, δ
c1,0, δ
c2,0, δ
t1, δ
t2, δ
nS1, δ
nS2, LA
0, LA
7bring equation into
,
,
,
, calculate the share that plant utilizes total carbonic acid hydrogen radical ion
f b; 7th, measure the Net Photosynthetic Rate of investigated plant blade
p n, be the photosynthesis assimilation ability of plant to atmospheric carbon dioxide; 8th, the Net Photosynthetic Rate value recorded
p ntotal carbonic acid hydrogen radical ion share is utilized with the plant calculated
f bsubstitute into equation
, calculate plant to the photosynthesis assimilation ability of total carbonic acid hydrogen radical ion; 9th, the plant measured to the photosynthesis assimilation ability of atmospheric carbon dioxide
p nwith the plant recorded, equation is substituted into the photosynthesis assimilation ability BUC of total carbonic acid hydrogen radical ion
p n'=
p n+ BUC, calculates plant total carbon assimilative capacity.
advantage of the present invention is as follows:
1) the present invention can not only record the equipment usage of plant to the bicarbonate ion that external source is added, and can record plant to the share utilized from the lysigenous bicarbonate ion of atmospheric carbon dioxide.Overcome prior art and underestimate the defect of plant to bicarbonate ion Utilization ability.
2) this method can record plant total carbon assimilative capacity, not only comprises the assimilative capacity of plant to atmospheric carbon dioxide, but also includes the assimilative capacity of plant to the bicarbonate ion from root absorption.
3) this method carries out two culture experiment under identical experiment condition simultaneously, therefore, obtains plant more reliable to the data of total carbon assimilative capacity.
4) this method eliminates always " carbon " to the impact of measurement result, and therefore, the result degree of accuracy of mensuration is high.
inventive principle
The strong fractionation feature of stable carbon isotope is the basis identifying different inorganic carbon source in plant.Occurring in nature carbon has two kinds of stable isotopes:
12c and
13c, their natural average abundance is respectively 98.89% and 1.11%.Stable carbon isotope composition uses δ usually
13c(‰) represent, occurring in nature δ
13c is changed to-90 ‰ ~+20 ‰.The strong fractionation feature of stable carbon isotope is conducive to identifying different inorganic carbon source in plant.Mass balance principle and isotopic dating method and chemometrics method are the bases in different inorganic carbon source in quantitative judge plant.
Isotope two end member mixture model can be expressed as:
δ
T=δ
A-
f Bδ
A+
f Bδ
B(1)
Here δ
tfor the δ of investigated plant blade
13c value, δ
afor supposition plant utilizes atmospheric carbon dioxide for the δ of blade during sole carbon source
13c value, δ
bfor the δ supposing that the bicarbonate ion that plant utilizes external source to add completely is blade during sole carbon source
13c value,
f bthe bicarbonate ion utilizing external source to add for this investigation plant accounts for the share that plant utilizes total inorganic carbon (TIC) source.
Obviously, only δ is known
tbe difficult to obtain
f b, therefore, the present invention adopts two-way carbon isotope labelling method to utilize two kinds of δ with larger difference
13with stable carbon isotope two-way mark, the C value bicarbonate ion plant that incubation growth is consistent simultaneously respectively, identifies that plant utilizes the share of different inorganic carbon source.
Because the present invention is the δ utilizing interpolation two kinds to have larger difference
13c value bicarbonate ion (isotope labeling 1 and the isotope labeling 2) plant that incubation growth is consistent simultaneously respectively, inorganic carbon source required for plant growth only includes atmospheric carbon dioxide and is added on the bicarbonate ion two kinds of inorganic carbon sources in nutrient solution, therefore, principle of the present invention is as follows:
The isotopic dating method of two end members:
δ
T=δ
A-
f Bδ
A+
f Bδ
B(1)
For isotope labeling 1, equation (1) is expressed as follows formula:
δ
T1=δ
A1-
f B1δ
A1+
f B1δ
B1(2)
Here δ
t1for with the first known δ
13the δ of the plant leaf blade of the bicarbonate ion cultivation of C value
13c value, δ
a1utilize carbon dioxide for the δ of blade during sole carbon source completely for being assumed to plant
13c value, δ
b1the first known δ is utilized completely for being assumed to plant
13the δ of blade when the bicarbonate ion of C value is sole carbon source
13c value,
f b1the first bicarbonate ion utilizing external source to add for this investigation plant accounts for the share of total carbon source that plant utilizes.
For isotope labeling 2, equation (1) is expressed as follows formula:
δ
T2=δ
A2-
f B2δ
A2+
f B2δ
B2(3)
Here δ
t2for with the known δ of the second
13the δ of the plant leaf blade of the bicarbonate ion cultivation of C value
13c value, δ
a2utilize carbon dioxide for the δ of blade during sole carbon source completely for being assumed to plant
13c value, δ
b2the known δ of the second is utilized completely for being assumed to plant
13the δ of blade when the bicarbonate ion of C value is sole carbon source
13c value,
f b2the second bicarbonate ion utilizing external source to add for this investigation plant accounts for the share of total carbon source that plant utilizes.
(2) δ and in (3) two equations
a1=δ
a2,
f b=
f b1=
f b2, simultaneous solution
(4)
(4) δ in formula
b1– δ
b2then can be converted into the δ of the bicarbonate ion of isotope labeling 1
13c value δ
c1with the δ of the bicarbonate ion of isotope labeling 2
13c value δ
c2difference, then (4) formula becomes:
(5)
Therefore, can by measuring the δ of the bicarbonate ion of isotope labeling 1
13c value δ
c1with the δ of the bicarbonate ion of isotope labeling 2
13c value δ
c2, the δ of the plant leaf blade of the bicarbonate ion cultivation of the mark that Simultaneously test is corresponding
13c value, namely determines δ
t1and δ
t2value, calculates the share of the bicarbonate ion that plant utilizes external source to add according to (5) formula
f b.
The share of the bicarbonate ion that the above-mentioned plant calculated utilizes external source to add
f brefer to that the first expanded leaves of investigated plant is to the equipment usage of the bicarbonate ion that external source is added, and the first expanded leaves now comprises " new carbon " and " old carbon ".In fact, " old carbon " part of this sheet leaf is the bicarbonate ion not utilizing external source to add, and only has " new carbon " part to be subject to the impact of the bicarbonate ion assimilation quotient that external source is added.Therefore, make use of the share that external source adds bicarbonate ion in the organic carbon of plant net increase
f bNbe then:
(6)
f bthe share calculating the bicarbonate ion that plant utilizes external source to add in above-mentioned (5) formula,
f lAfor investigated plant blade is in the increase ratio of cultivating organic carbon in certain hour.Here, the organic carbon of plant increases the ratio increase ratio of leaf area and represents, is:
(7)
f lAfor investigated plant blade is in the increase ratio of cultivating organic carbon in certain hour; LA
0and LA
7be respectively investigated plant blade the 0th day and cultivate the leaf area after 7 days.
In addition, carbon dioxide in air also can be dissolved in nutrient solution generate bicarbonate ion utilize by plant, this part bicarbonate ion is all left in the basket in existing technology by the share that plant utilizes, therefore, in order to record the equipment usage of plant to total carbonic acid hydrogen radical ion, also need to consider that atmospheric carbon dioxide is dissolved in nutrient solution the share generating bicarbonate ion and utilized by plant
f bNS.
Bicarbonate ion is directly proportional to the concentration of bicarbonate ion in solution by the share that plant utilizes, in order to record the bicarbonate ion concentration ratio in two kinds of sources in nutrient solution, isotope two end member mixture model can be utilized equally, an end member is the external source bicarbonate ion added, another end member is that atmospheric carbon dioxide is dissolved in the bicarbonate ion formed in nutrient solution, and to be formula be its expression formula:
δ
NS=δ
a-
f BNSδ
a+
f BNSδ
C(8)
Here δ
nSfor the δ of nutrient solution after cultivation plant certain hour
13c value, δ
afor atmospheric carbon dioxide is dissolved in the δ of the bicarbonate ion formed in nutrient solution
13c value, δ
cfor being added on the original delta of the external source bicarbonate ion in nutrient solution
13c value,
f bNSbicarbonate ion for external source interpolation after nutrient solution cultivation plant certain hour accounts for the share in nutrient solution total inorganic carbon (TIC) source.
For isotope labeling 1, equation (8) is expressed as follows formula:
δ
NS1=δ
a-
f BNS1δ
a+
f BNS1δ
C1,0(9)
Here δ
nS1for with the first known δ
13the δ of nutrient solution after the bicarbonate ion cultivation plant certain hour of C value
13c value, δ
afor atmospheric carbon dioxide is dissolved in the δ of the bicarbonate ion formed in nutrient solution
13c value, δ
c1,0for the bicarbonate ion of the first mark adds δ initial in nutrient solution to
13c value,
f bNS1the first bicarbonate ion for external source interpolation after cultivation plant certain hour accounts for the share in nutrient solution total inorganic carbon (TIC) source.
For isotope labeling 2, equation (8) is expressed as follows formula:
δ
NS2=δ
a-
f BNS2δ
a+
f BNS2δ
C2,0(10)
Here δ
nS2for with the known δ of the second
13the δ of nutrient solution after the bicarbonate ion cultivation plant certain hour of C value
13c value, δ
afor atmospheric carbon dioxide is dissolved in the δ of the bicarbonate ion formed in nutrient solution
13c value, δ
c2,0for the bicarbonate ion of the second mark adds δ initial in nutrient solution to
13c value,
f bNS2the second bicarbonate ion for external source interpolation after cultivation plant certain hour accounts for the share in nutrient solution total inorganic carbon (TIC) source.
(9) and in (10) two equations
f bNS=
f bNS1=
f bNS2, simultaneous solution
(11)
Therefore, δ initial in nutrient solution is dissolved into by measuring isotope labeling 1 with the bicarbonate ion of isotope labeling 2
13c value δ
c1,0and δ
c2,0, the δ of the nutrient solution after the bicarbonate ion cultivation plant certain hour of Simultaneously test correspondence markings
13c value, namely determines δ
nS1and δ
nS2value, the bicarbonate ion that after calculating cultivation plant certain hour according to (11) formula, external source is added accounts for the share in total inorganic carbon (TIC) source in nutrient solution
f bNS.
We suppose initially to add a certain amount of bicarbonate ion in nutrient solution, now in nutrient solution 100% be all external source add bicarbonate ion, but atmospheric carbon dioxide is dissolved in nutrient solution the bicarbonate ion generating bicarbonate ion and external source interpolation and is absorbed and used by plants, and the share that the bicarbonate ion that after cultivating certain hour, external source is added accounts for total inorganic carbon (TIC) source in nutrient solution is simultaneously
f bNS, the bicarbonate ion share utilizing external source to add in the organic carbon of plant net increase here
f bNutilize atmospheric carbon dioxide to be dissolved in nutrient solution to generate bicarbonate ion share sum with plant and be
fb, its expression formula is:
(12)
And the share of the bicarbonate ion that plant utilizes atmospheric carbon dioxide to be dissolved into be generated in nutrient solution is
f bCO2 – HCO3 -, its expression formula is:
(13)
Generally speaking, plant utilize total carbonic acid hydrogen radical ion (external source add bicarbonate ion and carbon dioxide solubility generate bicarbonate ion) share be
f balso can be expressed as:
(14)
f bfor the share of the bicarbonate ion that plant utilizes external source to add,
f bNSbicarbonate ion for external source interpolation after cultivation plant certain hour accounts for the share in total inorganic carbon (TIC) source in nutrient solution,
f lAfor investigating plant leaf blade in the increase ratio of cultivating leaf area in certain hour.
Above formula can calculate the share of the inorganic carbon source of difference that plant utilizes, then it also needs by photosynthetic instrument the photosynthesis assimilation ability of the inorganic carbon source of difference.Based on the plant leaf blade Net Photosynthetic Rate value that the photosynthetic instrument of atmospheric carbon dioxide flux measures
p nbe the photosynthetic carbon assimilative capacity of plant to atmospheric carbon dioxide, and plant can also utilize bicarbonate ion to carry out photosynthesis, it is expressed as BUC to the photosynthesis assimilation ability of bicarbonate ion, i.e. bicarbonate ion Utilization ability, and its expression formula is:
(15)
BUC is the photosynthesis assimilation ability of plant to bicarbonate ion,
p nfor plant is to the photosynthesis assimilation ability of atmospheric carbon dioxide,
f bfor plant utilizes the share of total carbonic acid hydrogen radical ion.
Therefore, plant total carbon assimilative capacity
p n' for plant is to the photosynthesis assimilation ability of atmospheric carbon dioxide
p nwith plant to bicarbonate ion photosynthesis assimilation ability BUC sum, its expression formula is:
P N'=
P N+BUC(16)。
Embodiment
Embodiments of the invention:
It comprises the following steps, and the first, the cave of indoor employing same specification dish water planting sprouts vegetable seeds, and preparation nutrient solution grows seedlings to 2 months, and the more consistent seedling of growth selection is as investigation plant seedlings; The second, measure the sodium bicarbonate that different manufacturers is produced, select two kinds of δ
13c value difference value is greater than the sodium bicarbonate of 10 ‰ as isotope labeling 1 and isotope labeling 2, and its value is respectively δ
c1and δ
c2, add in nutrient solution with 10mM amount respectively; The δ of bicarbonate ion in the nutrient solution of isotope labeling 1
13c value is δ
c1,0, the δ of bicarbonate ion in isotope labeling 2 nutrient solution
13c value is δ
c2,0; 3rd, the plant seedlings at use the nutrient solution of isotope labeling 1 and isotope labeling 2 respectively 2 monthly ages that incubation growth is consistent simultaneously, the corresponding nutrient solution more renewed every day; 4th, cultivate and start namely to cultivate the leaf area LA that the 0th day measures investigated plant first expanded leaves
0.Before renewing nutrient solution after 1 day, the stable carbon isotope composition δ of the nutrient solution that mensuration two kinds is isotope-labeled, corresponding respectively
13the value of C, δ
nS1and δ
nS2value; 5th, cultivate the leaf area LA measuring this first expanded leaves of investigated plant for 7 days afterwards
7, subsequently, measure the stable carbon isotope composition δ of this first expanded leaves of the plant that two kinds of isotope-labeled nutrient solutions are cultivated respectively
13the value of C, δ
t1and δ
t2; 6th, respectively by δ
c1, δ
c2, δ
c1,0, δ
c2,0, δ
t1, δ
t2, δ
nS1, δ
nS2, LA
0, LA
7bring equation into
,
,
,
, calculate the share that plant utilizes total carbonic acid hydrogen radical ion
f b; 7th, under indoor environment, utilize photosynthetic instrument to measure the Net Photosynthetic Rate of investigated plant blade
p n, be the photosynthesis assimilation ability of plant to atmospheric carbon dioxide; 8th, the Net Photosynthetic Rate value utilizing photosynthetic instrument to record
p ntotal carbonic acid hydrogen radical ion share is utilized with the plant calculated
f bsubstitute into equation
, calculate plant to the photosynthesis assimilation ability of total carbonic acid hydrogen radical ion; 9th, the plant measured to the photosynthesis assimilation ability of atmospheric carbon dioxide
p nwith the plant recorded, equation is substituted into the photosynthesis assimilation ability BUC of total carbonic acid hydrogen radical ion
p n'=
p n+ BUC, calculates plant total carbon assimilative capacity
p n'.
Detailed implementation process and content as follows:
The first step, the cave dish water planting of the same specification of indoor employing sprouts vegetable seeds, and preparation nutrient solution grows seedlings to 2 months, and the more consistent seedling of growth selection is as investigation plant seedlings;
Second step, measures the sodium bicarbonate that different manufacturers is produced, selects two kinds of δ
13c value difference value is greater than the sodium bicarbonate of 10 ‰ as isotope labeling 1 and isotope labeling 2, and its value is respectively δ
c1and δ
c2, add to respectively in nutrient solution; The δ of bicarbonate ion in the nutrient solution of isotope labeling 1
13c value is δ
c1,0, the δ of bicarbonate ion in isotope labeling 2 nutrient solution
13c value is δ
c2,0;
3rd step, the plant seedlings at use the nutrient solution of isotope labeling 1 and isotope labeling 2 respectively 2 monthly ages that incubation growth is consistent simultaneously, the corresponding nutrient solution more renewed every day;
4th step, cultivates and starts namely to cultivate the leaf area LA that the 0th day measures investigated plant first expanded leaves
0.Before renewing nutrient solution after 1 day, the stable carbon isotope composition δ of the nutrient solution that mensuration two kinds is isotope-labeled, corresponding respectively
13the value of C, δ
nS1and δ
nS2value;
5th step, cultivates the plant leaf blade stable carbon isotope composition δ measuring two kinds of isotope-labeled nutrient solutions cultivations after 7 days respectively
13the value of C, δ
t1and δ
t2value; The leaf area LA of this first expanded leaves of Simultaneously test investigated plant
7;
6th step, respectively by δ
c1, δ
c2, δ
c1,0, δ
c2,0, δ
t1, δ
t2, δ
nS1, δ
nS2, LA
0, LA
7bring equation into
,
,
,
, calculate the share that plant utilizes total carbonic acid hydrogen radical ion
f b;
7th step, utilizes photosynthetic instrument to measure the Net Photosynthetic Rate of investigated plant blade under indoor environment
p n, be the photosynthesis assimilation ability of plant to atmospheric carbon dioxide;
8th step, the Net Photosynthetic Rate value utilizing photosynthetic instrument to record
p ntotal carbonic acid hydrogen radical ion share is utilized with the plant calculated
f bsubstitute into equation
, calculate plant to the photosynthesis assimilation ability of total carbonic acid hydrogen radical ion;
9th step, the plant measured to the photosynthesis assimilation ability of atmospheric carbon dioxide
p nwith the plant recorded, equation is substituted into the photosynthesis assimilation ability BUC of total carbonic acid hydrogen radical ion
p n'=
p n+ BUC, calculates plant total carbon assimilative capacity
p n'.
Implementation result of the present invention is as follows:
Indoor employing 12 hole dish water planting sprouts Orychophragmus violaceus and mustard type rape two Plants seed, and preparation Huo Gelan nutrient solution grows seedlings large to 2 months, and the seedling that growth selection is more consistent is respectively as investigated plant seedling.Selection δ
13c value be-24.409 ‰ and-2.45 ‰ sodium bicarbonate as isotope labeling 1 and isotope labeling 2, respectively with 10mM amount add in Huo Gelan nutrient solution, be mixed with isotope labeling 1 nutrient solution and isotope labeling 2 nutrient solution.With isotope labeling 1 nutrient solution and isotope labeling 2 nutrient solution, the plant seedlings to 2 monthly ages is cultivated simultaneously respectively, the corresponding nutrient solution more renewed every day, within the 0th day, measures the leaf area LA of investigated plant first expanded leaves in cultivation
0, first day measures the stable carbon isotope composition δ of two kinds of isotope-labeled, corresponding nutrient solutions before changing nutrient solution
13the value of C; Cultivate after 7 days, measure the blade face product value LA of this first expanded leaves of plant that two kinds of isotope-labeled nutrient solutions are cultivated respectively
7and Net Photosynthetic Rate
p nvalue and stable carbon isotope composition δ
13the value of C.By the inventive method, draw Orychophragmus violaceus and mustard type rape two Plants total carbon photosynthesis assimilation ability respectively, as shown in the table.
Note: Zhu Ge Cai – 2 and juncea You Cai – 2 is for using carbon isotope labelling to be-2.45 ‰ (δ
c1) sodium bicarbonate treating fluid cultivate Orychophragmus violaceus and mustard type rape; Zhu Ge Cai – 24 and juncea You Cai – 24 is for using carbon isotope labelling to be-24.409 ‰ (δ
c2) sodium bicarbonate treating fluid cultivate Orychophragmus violaceus and mustard type rape.
Note: δ
c1,0and δ
c2,0the sodium bicarbonate being respectively carbon isotope labelling 1 and carbon isotope labelling 2 is dissolved into δ initial in nutrient solution
13c value; δ
nS1and δ
nS2the nutrient solution being respectively carbon isotope labelling 1 and carbon isotope labelling 2 cultivates the δ of plant after 1 day
13c value.
Note: in table, data are mean+/-standard error, the comparison between the different bicarbonate ion concentration of same parameters same plant adopts Multiple range test, and same letter represents that difference is not remarkable, different letter representation significant difference (
p<0.05).
Note: in table, data are mean+/-standard error, the comparison between the different bicarbonate ion concentration of same parameters same plant adopts Multiple range test, and same letter represents that difference is not remarkable, different letter representation significant difference (
p<0.05).
As can be seen from Table 3, when external source add sodium bicarbonate concentration be 5mM time, Orychophragmus violaceus external source add bicarbonate ion equipment usage be 2.27%, mustard type rape is 1.77%; When sodium bicarbonate concentration is 10mM, 7.06% of the bicarbonate ion equipment usage that the external source of Orychophragmus violaceus is added, and mustard type rape is only 2.11%; When sodium bicarbonate concentration is 15mM, 8.55% of the bicarbonate ion equipment usage that the external source of Orychophragmus violaceus is added, and mustard type rape is only 2.36%.The more important thing is, Orychophragmus violaceus utilizes total carbonic acid hydrogen radical ion (bicarbonate ion that external source is added and the bicarbonate ion that air carbon dioxide solubility generates to nutrient solution) share along with the increase of external source bicarbonate ion concentration is far above mustard type rape.This more can illustrate Orychophragmus violaceus comparatively mustard type rape more can survive under karst hydrocarbonate habitat, this result and Orychophragmus violaceus are that karst adaptive plant matches, clearly the ability of the Orychophragmus violaceus bicarbonate ion that utilizes external source to add is higher than mustard type rape, the more important thing is the assimilative capacity of two Plants total carbons
p n' the ratio that increases with blade and blade area
f lAthe increase of the bicarbonate ion concentration added along with external source is (table 4) that be proportionate, and this can this method illustration method be more reliable.
Claims (3)
1. one kind measures the method for plant gross photosynthesis carbon assimilation ability, it is characterized in that: it comprises the following steps: first, the cave dish water planting of the same specification of indoor employing sprouts vegetable seeds, and preparation nutrient solution grows seedlings to more than 2 months, and the more consistent seedling of growth selection is as investigation plant seedlings; The second, select two kinds of δ
13c value difference value is greater than the sodium bicarbonate of 10 ‰ as isotope labeling 1 and isotope labeling 2, and its value is respectively δ
c1and δ
c2, add to respectively in nutrient solution; The δ of bicarbonate ion in the nutrient solution of isotope labeling 1
13c value is δ
c1,0, the δ of bicarbonate ion in isotope labeling 2 nutrient solution
13c value is δ
c2,0; 3rd, the plant seedlings at use the nutrient solution of isotope labeling 1 and isotope labeling 2 respectively more than 2 months monthly ages that incubation growth is consistent simultaneously, the corresponding nutrient solution more renewed every day; 4th, cultivate and start namely to cultivate the leaf area LA that the 0th day measures investigated plant first expanded leaves
0; Before renewing nutrient solution after 1 day, the stable carbon isotope composition δ of the nutrient solution that mensuration two kinds is isotope-labeled, corresponding respectively
13the value of C, δ
nS1and δ
nS2value; 5th, cultivate the leaf area LA measuring this first expanded leaves of investigated plant for 7 days afterwards
7, subsequently, measure the stable carbon isotope composition δ of this first expanded leaves of the plant that two kinds of isotope-labeled nutrient solutions are cultivated respectively
13the value of C, δ
t1and δ
t2; 6th, utilize above-mentioned data measured, calculate the share that plant utilizes total carbonic acid hydrogen radical ion
f b; 7th, measure the Net Photosynthetic Rate of investigated plant blade
p n, be the photosynthesis assimilation ability of plant to atmospheric carbon dioxide; 8th, by the Net Photosynthetic Rate value recorded
p ntotal carbonic acid hydrogen radical ion share is utilized with the plant calculated
f bcalculate plant to the photosynthesis assimilation ability of total carbonic acid hydrogen radical ion; 9th, the photosynthesis assimilation ability of the plant recorded to atmospheric carbon dioxide
p nwith the plant recorded, equation is substituted into the photosynthesis assimilation ability BUC of total carbonic acid hydrogen radical ion
p n'=
p n+ BUC, calculates plant total carbon assimilative capacity
p n'.
2. a kind of method measuring plant gross photosynthesis carbon assimilation ability according to claim 1, is characterized in that: in step 6: respectively by δ
c1, δ
c2, δ
c1,0, δ
c2,0, δ
t1, δ
t2, δ
nS1, δ
nS2, LA
0, LA
7bring equation into
,
,
,
, calculate the share that plant utilizes total carbonic acid hydrogen radical ion
f b.
3. a kind of method measuring plant gross photosynthesis carbon assimilation ability according to claim 1, is characterized in that: in step 8: the Net Photosynthetic Rate value recorded
p ntotal carbonic acid hydrogen radical ion share is utilized with the plant calculated
f bsubstitute into equation
, calculate plant to the photosynthesis assimilation ability of total carbonic acid hydrogen radical ion.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105684899A (en) * | 2016-01-21 | 2016-06-22 | 中国科学院地球化学研究所 | Determining method of tissue cultured seedling sucrose |
| CN108319820A (en) * | 2018-04-09 | 2018-07-24 | 中国科学院地球化学研究所 | A kind of method of plant heavy carbonate equipment usage under acquisition outdoor habitatss |
| CN108333311A (en) * | 2018-02-12 | 2018-07-27 | 中国科学院地球化学研究所 | A kind of method of quick obtaining plant inorganic carbon supplement reuse share |
| CN108508156A (en) * | 2018-04-09 | 2018-09-07 | 中国科学院地球化学研究所 | A kind of method of plant inorganic carbon gross assimilation ability under acquisition outdoor habitatss |
| CN108801984A (en) * | 2018-05-21 | 2018-11-13 | 中国科学院地球化学研究所 | A kind of detection method of the plant inorganic nitrogen Assimilation rate based on Net Photosynthetic Rate |
| CN109444349A (en) * | 2018-12-26 | 2019-03-08 | 中国科学院地球化学研究所 | A method of measurement houseplant metabolic water equipment usage and practical water requirement |
| CN109900775A (en) * | 2019-03-22 | 2019-06-18 | 山东省农业科学院作物研究所 | A kind of method of in situ quantitation non-destructive testing wheat Ear photosynthesis contribution rate |
| CN111413465A (en) * | 2020-04-02 | 2020-07-14 | 沈阳农业大学 | Method for determining plant total photosynthetic carbon assimilation capacity |
| CN111983141A (en) * | 2020-07-22 | 2020-11-24 | 江苏大学 | Method for formulating crop irrigation strategy based on bicarbonate ion utilization capacity |
| CN113203837A (en) * | 2021-05-11 | 2021-08-03 | 浙江省园林植物与花卉研究所(浙江省萧山棉麻研究所) | Method for identifying main flow directions of carbon assimilation products of anthurium andraeanum in different development periods |
| CN113884621A (en) * | 2021-08-16 | 2022-01-04 | 中国科学院地球化学研究所 | Method for quantitatively absorbing and utilizing bicarbonate by plants |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19820078A1 (en) * | 1998-05-05 | 1999-11-11 | Fischer Analysen Instr Gmbh | Production of material labeled with carbon-13 useful for diagnostic or research purposes |
| CN101926267A (en) * | 2010-08-09 | 2010-12-29 | 中国科学院地球化学研究所 | Method for measuring bicarbonate ion utilizing capability of plant |
| EP2468848A2 (en) * | 2006-10-20 | 2012-06-27 | Arizona Board Regents For And On Behalf Of Arizona State University | Modified cyanobacteria |
| CN102511362A (en) * | 2011-10-27 | 2012-06-27 | 中国科学院地球化学研究所 | Method by utilizing double markers to acquire share of inorganic carbon source utilized by plants |
| CN102792891A (en) * | 2012-08-09 | 2012-11-28 | 中国科学院地球化学研究所 | Method for determining tissue culture seedling autotrophic portion |
| CN102899382A (en) * | 2012-10-23 | 2013-01-30 | 中国科学院地球化学研究所 | Method for quantifying indirect carbon sequestration ability of microalgae |
| CN103074411A (en) * | 2013-01-05 | 2013-05-01 | 中国科学院地球化学研究所 | Method for detecting and quantifying utilization of carbon source in calcium carbonate by microalgae |
| CN103616477A (en) * | 2013-11-13 | 2014-03-05 | 中国科学院地球化学研究所 | Method for measuring daily mean stable carbon isotope composition of atmospheric carbon dioxide |
-
2015
- 2015-08-10 CN CN201510482616.1A patent/CN105067772B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19820078A1 (en) * | 1998-05-05 | 1999-11-11 | Fischer Analysen Instr Gmbh | Production of material labeled with carbon-13 useful for diagnostic or research purposes |
| EP2468848A2 (en) * | 2006-10-20 | 2012-06-27 | Arizona Board Regents For And On Behalf Of Arizona State University | Modified cyanobacteria |
| CN101926267A (en) * | 2010-08-09 | 2010-12-29 | 中国科学院地球化学研究所 | Method for measuring bicarbonate ion utilizing capability of plant |
| CN102511362A (en) * | 2011-10-27 | 2012-06-27 | 中国科学院地球化学研究所 | Method by utilizing double markers to acquire share of inorganic carbon source utilized by plants |
| CN102792891A (en) * | 2012-08-09 | 2012-11-28 | 中国科学院地球化学研究所 | Method for determining tissue culture seedling autotrophic portion |
| CN102899382A (en) * | 2012-10-23 | 2013-01-30 | 中国科学院地球化学研究所 | Method for quantifying indirect carbon sequestration ability of microalgae |
| CN103074411A (en) * | 2013-01-05 | 2013-05-01 | 中国科学院地球化学研究所 | Method for detecting and quantifying utilization of carbon source in calcium carbonate by microalgae |
| CN103616477A (en) * | 2013-11-13 | 2014-03-05 | 中国科学院地球化学研究所 | Method for measuring daily mean stable carbon isotope composition of atmospheric carbon dioxide |
Non-Patent Citations (4)
| Title |
|---|
| DEKE XING,ET AL.: "Effect of phosphorus deficiency on photosyntheticinorganic carbon assimilation of three climber plant species", 《BITANICAL STUDIES》 * |
| Y.Y. WU, ET AL.: "Effect of bicarbonate treatment on photosynthetic assimilation of inorganic carbon in two plant species of Moraceae", 《PHOTOSYNTHETICA》 * |
| 吴沿友 等: "植物利用碳酸氢根离子的特征分析", 《地球与环境》 * |
| 杭红涛 等: "双向标记培养植物测定大气二氧化碳稳定碳同位素组成", 《广西植物》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105684899A (en) * | 2016-01-21 | 2016-06-22 | 中国科学院地球化学研究所 | Determining method of tissue cultured seedling sucrose |
| CN105684899B (en) * | 2016-01-21 | 2018-09-21 | 中国科学院地球化学研究所 | The assay method of tissue-cultured seedling sucrose utilization rate |
| CN108333311A (en) * | 2018-02-12 | 2018-07-27 | 中国科学院地球化学研究所 | A kind of method of quick obtaining plant inorganic carbon supplement reuse share |
| CN108319820A (en) * | 2018-04-09 | 2018-07-24 | 中国科学院地球化学研究所 | A kind of method of plant heavy carbonate equipment usage under acquisition outdoor habitatss |
| CN108508156A (en) * | 2018-04-09 | 2018-09-07 | 中国科学院地球化学研究所 | A kind of method of plant inorganic carbon gross assimilation ability under acquisition outdoor habitatss |
| CN108801984A (en) * | 2018-05-21 | 2018-11-13 | 中国科学院地球化学研究所 | A kind of detection method of the plant inorganic nitrogen Assimilation rate based on Net Photosynthetic Rate |
| CN109444349A (en) * | 2018-12-26 | 2019-03-08 | 中国科学院地球化学研究所 | A method of measurement houseplant metabolic water equipment usage and practical water requirement |
| CN109900775A (en) * | 2019-03-22 | 2019-06-18 | 山东省农业科学院作物研究所 | A kind of method of in situ quantitation non-destructive testing wheat Ear photosynthesis contribution rate |
| CN111413465A (en) * | 2020-04-02 | 2020-07-14 | 沈阳农业大学 | Method for determining plant total photosynthetic carbon assimilation capacity |
| CN111983141A (en) * | 2020-07-22 | 2020-11-24 | 江苏大学 | Method for formulating crop irrigation strategy based on bicarbonate ion utilization capacity |
| CN113203837A (en) * | 2021-05-11 | 2021-08-03 | 浙江省园林植物与花卉研究所(浙江省萧山棉麻研究所) | Method for identifying main flow directions of carbon assimilation products of anthurium andraeanum in different development periods |
| CN113884621A (en) * | 2021-08-16 | 2022-01-04 | 中国科学院地球化学研究所 | Method for quantitatively absorbing and utilizing bicarbonate by plants |
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