CN105067746B - The assay method of padimate in cosmetics - Google Patents
The assay method of padimate in cosmetics Download PDFInfo
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- CN105067746B CN105067746B CN201510475211.5A CN201510475211A CN105067746B CN 105067746 B CN105067746 B CN 105067746B CN 201510475211 A CN201510475211 A CN 201510475211A CN 105067746 B CN105067746 B CN 105067746B
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Abstract
The invention discloses the assay method of padimate in a kind of cosmetics, it comprises the steps: the pre-treatment of (1) sample；(2) use the method for high performance liquid chromatography that padimate is analyzed, optimize high-efficient liquid phase chromatogram condition；(3) draw standard working curve, use quantified by external standard method, calculate the content of padimate.Padimate A and PADIMATE O during in cosmetics of the present invention, the assay method of padimate uses hplc simultaneous determination cosmetics, for using the sample of the high performance liquid chromatography detection positive, high performance liquid chromatography mass spectrum/mass spectrography is used to confirm further.The method of the invention is accurate, quick, highly sensitive, and the quality safety Risk Monitoring for cosmetics is significant.
Description
Technical field
The present invention relates to the assay method of a kind of chemical substance, particularly relate to the assay method of padimate in cosmetics.
Background technology
Padimate is divided into Padimate A and PADIMATE O.Padimate A, relative molecular mass: 235.36, CAS:
14779-78-3, molecular formula: C14H21NO2, structural formula is as follows:
PADIMATE O, relative molecular mass: 277.41, CAS:21245-02-3, molecular formula: C17H27NO2, structural formula
As follows:
Padimate A has stimulation due to it to eyes, respiratory tract and skin, in China's " cosmetics health specification " clearly
Must not add Padimate A in regulation cosmetics, PADIMATE O is as a kind of anti-ultraviolet absorbent, being widely used in
In cosmetic and other printing opacity anti-Exposure to Sunlight industrial goods, limitation is 8%.Both at home and abroad the detection method of padimate in cosmetics is not appeared in the newspapers
Road.
Summary of the invention
The technical problem to be solved in the present invention is to provide the mensuration of padimate in a kind of accurate, quick, highly sensitive cosmetics
Method.
The assay method of padimate in cosmetics, it comprises the steps:
(1) pre-treatment of sample；
(2) use the method for high performance liquid chromatography that padimate is analyzed, optimize high-efficient liquid phase chromatogram condition；
(3) draw standard working curve, use quantified by external standard method, calculate the content of padimate.
The assay method of padimate in cosmetics of the present invention, wherein, described high-efficient liquid phase chromatogram condition is as follows:
Chromatographic column: XTerra C18(4.6mm×250mm,5μm)；
Flow velocity: 1.0mL/min；
Flowing phase: acetonitrile-water mixed solution, wherein acetonitrile is 9:1 with the volume ratio of water, isocratic elution；
Detection wavelength: 310nm；
Column temperature: 30 DEG C；
Sample size: 10 μ L.
The assay method of padimate in cosmetics of the present invention, wherein, described sample is cream kind, lip pomade class, water preparation
Class, loose powder class or shampoo class cosmetic sample.
The assay method of padimate in cosmetics of the present invention, wherein, described sample is cream kind cosmetic sample, front
Process comprises the steps: that taking 1g sample is placed in 10mL color comparison tube, after adding 2g sodium chloride breakdown of emulsion, adds methanol to carving
Degree, fully mixes, and ultrasonic assistant extracts 20min, is transferred in centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed,
Taking supernatant and cross film, filtrate is for high-performance liquid chromatogram determination.
The assay method of padimate in cosmetics of the present invention, wherein, described sample is lip pomade class cosmetic sample, front
Process comprises the steps: that taking 1g sample is placed in 10mL color comparison tube, add 2mL oxolane, add methanol to scale,
Fully mixing, ultrasonic assistant extracts 20min, is transferred in centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed, takes
Supernatant crosses film, and filtrate is for high-performance liquid chromatogram determination.
The assay method of padimate in cosmetics of the present invention, wherein, described sample is water preparation class, loose powder class or shampoo
Class cosmetic sample, pre-treatment comprises the steps: that taking 1g sample is placed in 10mL color comparison tube, add methanol to scale,
Fully mixing, ultrasonic assistant extracts 20min, is transferred in centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed, takes
Supernatant crosses film, and filtrate is for high-performance liquid chromatogram determination.
The assay method of padimate in cosmetics of the present invention, wherein, for using the high performance liquid chromatography detection positive
Sample, use High Performance Liquid Chromatography/Mass Spectrometry/mass spectrography to confirm, wherein further:
High-efficient liquid phase chromatogram condition is as follows:
Chromatographic column: HSS T3 chromatographic column (100mm × 2.1mm, 1.8 μm)；
Flowing phase: volume ratio is 0.1% aqueous formic acid and the methanol of 1:9；
Flow velocity: 0.4mL/min；
Column temperature: 30 DEG C；
Sample size: 10 μ L；
Mass Spectrometry Conditions is as follows:
Ion source: electric spray ion source；
Ionization mode: positive ion mode；
Capillary voltage: 3.5kV；
Extraction voltage: 2.9V；
Ion source temperature: 150 DEG C；
Desolventizing temperature: 350 DEG C；
Data acquisition modes: many reactive ions are monitored.
The assay method of padimate in cosmetics of the present invention, wherein, described padimate is Padimate A and handkerchief ground
Horse ester O.
The assay method of padimate in cosmetics of the present invention, wherein, described confirmatory test comprises the steps: sample
Liquid suitably dilutes, and measures sample liquid and standard working solution by High Performance Liquid Chromatography/Mass Spectrometry/Mass Spectrometry Conditions, if selected ion is equal
Occur, and selected ion ratio be consistent with the relative abundance of standard substance, it is allowed to the scope that deviation specifies less than table 1,
Then can determine whether in sample containing padimate.
Table 1 padimate mass spectral analysis parameter
In cosmetics of the present invention, the assay method difference from prior art of padimate is: there is no cosmetics both at home and abroad at present
The assay method report of middle Padimate A and PADIMATE O, in cosmetics of the present invention, the assay method of padimate uses efficiently
Liquid chromatography measures Padimate A and PADIMATE O in cosmetics simultaneously, accurate, quick, highly sensitive, for making up
The quality safety Risk Monitoring of product is significant.
Below in conjunction with the accompanying drawings the assay method of padimate in the cosmetics of the present invention is described further.
Accompanying drawing explanation
Fig. 1 is the ultra-violet absorption spectrum of padimate in the present invention；
Fig. 2 is employing Elipes XDB C in the present invention18The chromatogram that (4.6mm × 250mm, 5 μm) chromatographic column obtains；
Wherein, 1-Padimate A, 2-PADIMATE O；
Fig. 3 is employing XTerra C in the present invention18The chromatogram that (4.6mm × 250mm, 5 μm) chromatographic column obtains；Wherein,
1-Padimate A, 2-PADIMATE O；
Fig. 4 is employing Promosil C in the present invention18The chromatogram that (4.6mm × 250mm, 5 μm) chromatographic column obtains；Wherein,
1-Padimate A, 2-PADIMATE O；
Fig. 5 is employing Symmetry C in the present invention18The chromatogram that (4.6mm × 250mm, 5 μm) chromatographic column obtains；Its
In, 1-Padimate A, 2-PADIMATE O；
Fig. 6 is employing Kromasil C in the present invention18The chromatogram that (4.6mm × 250mm, 5 μm) chromatographic column obtains；Wherein,
1-Padimate A, 2-PADIMATE O；
Fig. 7 is the multiple-reaction monitoring chromatogram of padimate in the present invention, wherein, 1-Padimate A, 2-PADIMATE O.
The all of English corresponding translator of Chinese occurred in accompanying drawing of the present invention is:
MAU: absorption value in the least；Time: the time；Abs: trap；Nm: nanometer；Response: response value.
Detailed description of the invention
Embodiment 1
One, reagent and material
Except as otherwise noted, agents useful for same is analytical pure, and water is the experimental water that GB/T 6682-2008 specifies.
Methanol: chromatographically pure.
Oxolane: chromatographically pure.
Sodium chloride.
Padimate standard substance.
Padimate hybrid standard storing solution: weigh appropriate Padimate A respectively and PADIMATE O standard substance (are accurate to 0.0001
G), being configured to the list mark storing solution containing methanol 1000mg/L with methanol, again with methanol is configured to the hybrid standard deposit of 100mg/L
Liquid.
Padimate hybrid standard working solution: with methanol padimate hybrid standard storing solution is configured to concentration be 0.02,0.05,
0.1,0.2,0.5,1,2,5,10mg/L methanol mixed standard working solution.
Two, instrument and equipment
High performance liquid chromatograph: be furnished with UV-detector.
Analytical balance: sensibility reciprocal is 0.0001g and 0.001g.
Centrifuge: rotating speed is not less than 5000r/min.
Ultrasound bath
Syringe: 5mL.
Microporous filter membrane: 0.45 μm, organic facies.
Three, analytical procedure
1, sample treatment
(1) cream kind cosmetic sample pre-treatment
Taking 1g sample to be placed in 10mL color comparison tube, after adding 2g sodium chloride breakdown of emulsion, addition methanol, to scale, fully mixes,
Ultrasonic assistant extracts 20min, is transferred in centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed, takes supernatant and cross film,
Filtrate is for high-performance liquid chromatogram determination.
(2) lip pomade class cosmetic sample pre-treatment.
Take 1g sample to be placed in 10mL color comparison tube, add 2mL oxolane, add methanol to scale, fully mix,
Ultrasonic assistant extracts 20min, is transferred in centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed, takes supernatant and cross film,
Filtrate is for high-performance liquid chromatogram determination.
(3) water preparation class, loose powder class and shampoo class cosmetic sample pre-treatment
Taking 1g sample to be placed in 10mL color comparison tube, addition methanol, to scale, fully mixes, and ultrasonic assistant extracts 20min,
Being transferred in centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed, take supernatant and cross film, filtrate is surveyed for high performance liquid chromatography
Fixed.
2, condition determination
High performance liquid chromatography reference conditions:
Chromatographic column: XTerra C18(4.6mm×250mm,5μm)；
Flow velocity: 1.0mL/min；
Flowing phase: acetonitrile-water mixed solution, wherein acetonitrile is 9:1 with the volume ratio of water, isocratic elution；
Detection wavelength: 310nm；
Column temperature: 30 DEG C；
Sample size: 10 μ L.
3, the drafting of standard curve
Padimate standard working solution is measured by as above condition determination sample introduction the most from low to high, with peak area-concentration mapping,
To standard regressive method, draw standard working curve.
4, measure
By condition determination of the present invention, testing sample is measured, uses quantified by external standard method.Treat that in sample measuring liquid, padimate content should
Within standard curve, it is being analyzed after then should diluting beyond the range of linearity.For using the high performance liquid chromatography detection positive
Sample, uses High Performance Liquid Chromatography/Mass Spectrometry/mass spectrography to confirm further.
5, blank assay
In addition to not weighing sample, all carry out by said determination condition and step.
6, result calculates
In formula:
The content of padimate, mg/kg in W sample；
The concentration of padimate, mg/L in the sample solution that c finds from standard working curve；
The final constant volume of V sample liquid, mL；
The quality of m sample, g.
7, detection limit and quantitative limit
The detection of padimate is limited to 0.2mg/kg, is quantitatively limited to 0.5mg/kg.
8, the response rate
In adding concentration 0.5mg/kg～80000mg/kg concentration range, record the response rate 89.15%～112.57%, relatively
Standard deviation is 1.91%～7.71%.
Four, the selection of experiment condition:
1, the selection of wavelength is detected
Measuring Padimate A and the maximum absorption wavelength of PADIMATE O, sweep limits is 200-400nm.After measured, as
Shown in Fig. 1, Padimate A and PADIMATE O all have maximum absorption band at 310nm.Therefore, its detection wavelength is set
For 310nm.
2, the optimization of chromatographic column
Investigate Elipes XDB C respectively18(4.6mm×250mm,5μm)、XTerra C18(4.6mm×250mm,5
μm)、Promosil C18(4.6mm×250mm,5μm)、Symmetry C18(4.6mm × 250mm, 5 μm) and
Kromasil C18(4.6mm × 250mm, 5 μm) opposed polarity chromatographic column on padimate peak shape and the impact of retention time,
Result is as shown in figures 2-6.Result shows, with acetonitrile: water=90:10 is for flowing under phase elution requirement, and various chromatographic columns are to handkerchief
Ground horse ester eluting peak shape is hangover, peak shape and peak area zero difference, and XTerra C18(4.6mm×250mm,5μm)
The most reasonable to its retention time, go out peak very fast, therefore select XTerra C18(4.6mm × 250mm, 5 μm) are chromatographic column.
The optimization of the phase that 3, flows
Select methanol respectively: water=50:50, methanol: water=80:20, methanol: water=90:10, acetonitrile: water=80:20, acetonitrile:
Water=90:10 tetra-kinds flows phase, investigates the different eluting solvent impact on padimate.Result shows, selection acetonitrile: water=90:10
As flowing phase, padimate eluting effect peak shape is best, and retention time is the shortest, therefore flows and the most finally selects acetonitrile: water
=90:10.
4, chromatogram column temperature optimization
Investigate chromatogram column temperature and padimate is gone out the impact at peak.Select respectively 25 DEG C, 30 DEG C, 35 DEG C, under the conditions of 40 DEG C,
With acetonitrile: water=90:10 is flowing phase, XTerra C18(4.6mm × 250mm, 5 μm) chromatographic column is analytical column, result table
Bright, at each temperature, padimate retention time zero difference, and under the conditions of column temperature 30 DEG C, padimate peak shape is best, because of
This column temperature selects 30 DEG C.
5, the selection of Extraction solvent
Relatively methanol and the acetonitrile impact on padimate extraction effect.Screening is after measured without Padimate A and PADIMATE O
Cream kind, water preparation class, loose powder class, lip pomade class and shampoo class cosmetic sample as blank sample, be added thereto to a certain amount of
After padimate standard solution, fully mixing, extract padimate by above-mentioned steps.Result shows, using methanol and acetonitrile as
Sample extraction solution, to padimate extraction ratio zero difference.In view of the toxicity of acetonitrile and relatively costly, finally determine that methanol is
Sample extraction solvent.
Five, the linear relationship of method and quantitative limit
1, linear relationship
Under experiment condition determined by this standard method, the Padimate A of preparation 1000mg/L and the storage of PADIMATE O standard
Standby liquid, afterwards preparation 100mg/L hybrid standard storing solution.Be diluted to 0.02 with methanol, 0.05,0.1,0.2,0.5,1,
2、5、10mg/L；With peak area as vertical coordinate, corresponding padimate concentration is abscissa mapping, handkerchief in the range of concentrations above
The linear equation of ground horse ester A is y=56.564x-0.1024, correlation coefficient r2It is 0.9999；The linear equation of PADIMATE O
For y=51.417x-0.1266, correlation coefficient r2It is 0.9999, all in good linear relationship.
2, the detection limit of method and quantitative limit
Through the laboratory experiment of the method for inspection, result shows, the detection limit (S/N=3) of method is 0.2mg/kg, quantitative limit
(S/N=10) be the quantitative limit high-efficient liquid phase chromatogram of 0.5mg/kg, Padimate A and PADIMATE O as shown in Figure 7.
Six, the response rate of method and precision
This standard method sets 0.5mg/kg, 5mg/kg, 10mg/kg tri-for Padimate A recovery testu and adds
Add concentration, 0.5mg/kg, 500mg/kg, 80000mg/kg tri-interpolation is set for PADIMATE O recovery testu
Concentration.Treat in sample measuring liquid that padimate content should be analyzed after then should diluting beyond the range of linearity within standard curve.
Experiment condition as determined by this method of inspection, to each interpolation concentration in cream kind, water preparation class, lip pomade class, powder class and shampoo
Class sample has all carried out 6 tests, records the response rate 89.15～112.57%, and precision test records relative standard deviation and is
1.91～7.71%.Result is summarized in table 2-6.
The table 2 cream kind cosmetic sample response rate and Precision test result
The table 3 water preparation class cosmetic sample response rate and Precision test result
The table 4 lip pomade class cosmetic sample response rate and Precision test result
The table 5 loose powder class cosmetic sample response rate and Precision test result
The table 6 shampoo class cosmetic sample response rate and Precision test result
Seven, actual sample detection
The method that application is set up, the different brands that the number of ways such as commercially available, net purchase are obtained, 44 cosmetics samples of different class
Product carry out the mensuration of padimate content, wherein 11, cream kind sample, 8, water preparation class sample, 8, lip pomade class sample, dissipate
8, powder class sample, 9, shampoo class sample.Result shows, does not all detect Padimate A and padimate in surveyed cosmetics
O。
Eight, confirmatory test
For using the sample of the high performance liquid chromatography detection positive, High Performance Liquid Chromatography/Mass Spectrometry/mass spectrography is used to carry out further
Confirmation.
High-efficient liquid phase chromatogram condition is as follows:
Chromatographic column: HSS T3 chromatographic column (100mm × 2.1mm, 1.8 μm)；
Flowing phase: volume ratio is 0.1% aqueous formic acid and the methanol of 1:9；
Flow velocity: 0.4mL/min；
Column temperature: 30 DEG C；
Sample size: 10 μ L；
Mass Spectrometry Conditions is as follows:
Ion source: electric spray ion source；
Ionization mode: positive ion mode；
Capillary voltage: 3.5kV；
Extraction voltage: 2.9V；
Ion source temperature: 150 DEG C；
Desolventizing temperature: 350 DEG C；
Data acquisition modes: many reactive ions are monitored.
The assay method of padimate in cosmetics of the present invention, wherein, described padimate is Padimate A and handkerchief ground
Horse ester O.
The assay method of padimate in cosmetics of the present invention, wherein, described confirmatory test comprises the steps: sample
Liquid suitably dilutes, and measures sample liquid and standard working solution by High Performance Liquid Chromatography/Mass Spectrometry/Mass Spectrometry Conditions, if selected ion is equal
Occur, and selected ion ratio be consistent with the relative abundance of standard substance, it is allowed to the scope that deviation specifies less than table 1,
Then can determine whether in sample containing padimate.
Table 1 padimate mass spectral analysis parameter
Embodiment described above is only to be described the preferred embodiment of the present invention, not carries out the scope of the present invention
Limiting, on the premise of designing spirit without departing from the present invention, technical scheme is made by those of ordinary skill in the art
Various deformation and improvement, all should fall in the protection domain that claims of the present invention determines.
Claims (2)
1. the assay method of padimate in cosmetics, it is characterised in that: comprise the steps:
(1) pre-treatment of sample；
(2) use the method for high performance liquid chromatography that padimate is analyzed, optimize high-efficient liquid phase chromatogram condition；
(3) draw standard working curve, use quantified by external standard method, calculate the content of padimate；
Described high-efficient liquid phase chromatogram condition is as follows:
Chromatographic column: XTerra C18, 4.6mm × 250mm, 5 μm；
Flow velocity: 1.0mL/min；
Flowing phase: acetonitrile-water mixed solution, wherein acetonitrile is 9:1 with the volume ratio of water, isocratic elution；
Detection wavelength: 310nm；
Column temperature: 30 DEG C；
Sample size: 10 μ L；
Described padimate is Padimate A and PADIMATE O；
Described sample is cream kind, lip pomade class, water preparation class, loose powder class or shampoo class cosmetic sample；
When described sample is cream kind cosmetic sample, pre-treatment comprises the steps: that taking 1g sample is placed in 10mL ratio
In colour tube, after adding 2g sodium chloride breakdown of emulsion, addition methanol, to scale, fully mixes, and ultrasonic assistant extracts 20min,
Being transferred in centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed, take supernatant and cross film, filtrate is for high performance liquid chromatography
Measure；
When described sample is lip pomade class cosmetic sample, pre-treatment comprises the steps: that taking 1g sample is placed in 10mL ratio
In colour tube, adding 2mL oxolane, add methanol to scale, fully mix, ultrasonic assistant extracts 20min,
Being transferred in centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed, take supernatant and cross film, filtrate is for high performance liquid chromatography
Measure；
When described sample is water preparation class, loose powder class or shampoo class cosmetic sample, pre-treatment comprises the steps: to take 1g
Sample is placed in 10mL color comparison tube, and addition methanol, to scale, fully mixes, and ultrasonic assistant extracts 20min, is transferred to
In centrifuge tube, after being centrifuged 15min with 5000r/min rotating speed, taking supernatant and cross film, filtrate is for high-performance liquid chromatogram determination.
The assay method of padimate in cosmetics the most according to claim 1, it is characterised in that: for using height
The sample that the detection of effect liquid phase chromatogram method is positive, uses High Performance Liquid Chromatography/Mass Spectrometry/mass spectrum confirmation method to confirm further；
Described High Performance Liquid Chromatography/Mass Spectrometry/mass spectrum confirmation method comprises the steps: suitably to dilute sample liquid, by efficiently
Liquid chromatography-mass spectrography/Mass Spectrometry Conditions measures sample liquid and standard working solution；
Described High Performance Liquid Chromatography/Mass Spectrometry/Mass Spectrometry Conditions is as follows:
Chromatographic column: HSS T3 chromatographic column, 100mm × 2.1mm, 1.8 μm；
Flowing phase: volume ratio is 0.1% aqueous formic acid and the methanol of 1:9；
Flow velocity: 0.4mL/min；
Column temperature: 30 DEG C；
Sample size: 10 μ L；
Mass Spectrometry Conditions is as follows:
Ion source: electric spray ion source；
Ionization mode: positive ion mode；
Capillary voltage: 3.5kV；
Extraction voltage: 2.9V；
Ion source temperature: 150 DEG C；
Desolventizing temperature: 350 DEG C；
Data acquisition modes: many reactive ions are monitored；
Padimate mass spectral analysis parameter is:
If selected ion all occurs, and selected ion ratio is consistent with the relative abundance of standard substance, it is allowed to phase
To the deviation scope less than ± 20%, then containing padimate in judgement sample.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101863782A (en) * | 2010-06-25 | 2010-10-20 | 北京英力精化技术发展有限公司 | Method for synthesizing ultraviolet photoinitiator of p-dimethylamin benzoic ether compounds |
| CN102818874A (en) * | 2012-08-31 | 2012-12-12 | 国家烟草质量监督检验中心 | Detection method of determining 16 types of photoinitiators in paper printing and packaging material |
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| SE508191C2 (en) * | 1996-03-25 | 1998-09-14 | Nordic Synthesis Ab | Liquid mixtures of benzene derivatives with good odor properties as photoinitiators in radiation curing |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101863782A (en) * | 2010-06-25 | 2010-10-20 | 北京英力精化技术发展有限公司 | Method for synthesizing ultraviolet photoinitiator of p-dimethylamin benzoic ether compounds |
| CN102818874A (en) * | 2012-08-31 | 2012-12-12 | 国家烟草质量监督检验中心 | Detection method of determining 16 types of photoinitiators in paper printing and packaging material |
Non-Patent Citations (2)
| Title |
|---|
| High-performance liquid chromatographic assay for common sunscreening agents in cosmetic products, bovine serum albumin solution and human plasma;R. Jiang等;《Journal of Chromatography B》;19961231;第682卷;137-145 * |
| 使用UPLC/Q-Tof液质仪对食品包装纸和纸板中潜在迁移物进行鉴别和分析;Malcolm Driffield等;《食品安全导刊》;20130630(第6期);35-37 * |
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