CN105053755A - Method for effectively removing heavy metals from shellfish protein enzymatic hydrolysate - Google Patents
Method for effectively removing heavy metals from shellfish protein enzymatic hydrolysate Download PDFInfo
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- CN105053755A CN105053755A CN201510491966.4A CN201510491966A CN105053755A CN 105053755 A CN105053755 A CN 105053755A CN 201510491966 A CN201510491966 A CN 201510491966A CN 105053755 A CN105053755 A CN 105053755A
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Abstract
The invention discloses a method for effectively removing heavy metals from a shellfish protein enzymatic hydrolysate. The method comprises the following steps of (1) dissolving phytic acid in water to prepare a phytic acid aqueous solution; (2) sufficiently mixing the phytic acid aqueous solution with diatomaceous earth to obtain paste, heating and drying the paste, and finely grinding the paste to obtain a phytic acid-loaded diatomaceous earth heavy metal adsorbent; and (3) adding the phytic acid-loaded diatomaceous earth heavy metal adsorbent into the shellfish protein enzymatic hydrolysate, regulating the pH value of the mixture, sufficiently stirring the mixture, carrying out adsorbing and centrifuging, and filtering the precipitation out. The method can be used for simultaneously reducing the content of arsenic, cadmium, lead, mercury and other heavy metals in the shellfish enzymatic hydrolysate, and accords with the standard of pollutant limit in foods of the National Food Safety Standard GB2762-2012. The method has the advantages of simple operation, low cost, short reaction time, no environmental pollution and high product recycling rate, can be used for easily separating heavy metal complexes, and is suitable for industrial operation.
Description
Technical field
The present invention relates to a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal.
Background technology
China is shellfish culture big country of the world, and annual shellfish output reaches 1,000 ten thousand tons, accounts for 60% of total output.Marine shellfish cultured output accounts for sea-farming total output 80%, and the shellfish by nest RT-PCR reaching large-scale production level at present mainly contains oyster, clam, scallop, razor clam and mussel etc.Along with economic development, the discharge of industrial wastewater, municipal sewage makes paralic environment be seriously damaged.Seashells because of its activity poor, be easily exposed in polluted water region, simultaneously due to its filter-feeding, compare other biological heavy metal and there is stronger accumulation ability, be more easily subject to heavy metal pollution and cause heavy metals exceeding standard.Disease Control and Prevention Center of Shenzhen discloses an item number certificate, the detection display to 21 based foods in 2012, and the shellfish food heavy metals exceeding standard situation in aquatic products is serious, and in 69 increment product of sampling observation, seventy percent cadmium content exceeds standard.Comprising the shellfish aquatic products such as belt, scallop, first shellfish, blood clam, raw oyster, cycle of sixty years, Sha Bai, Holy Son-Jesus Christ, abalone, clam all has cadmium to exceed standard sample.That cadmium content detected value is the highest is 21.4mg/kg, exceeds national safety standard 10 times.A large amount of the survey showed that, and the heavy metal problem of China shellfish is particularly outstanding, is mainly manifested in cadmium and lead doping phenomenon is serious, and the cadmium content of most shellfish all exceedes " human physiology consumption standard ".These pollute the food security that directly affects people, and the Deep processing pedagogy that have impact on seashells protein utilizes; Also have impact on China's seafood exportation simultaneously, local economy is caused damage.In effective control seashells, heavy metal pollution has become a problem demanding prompt solution.
Removal methods at present for seashells heavy metal has live body shellfish purification, shellfish protein enzymolysis liquid heavy metal removes both direction.Live body shellfish purification technology refers in the water environment by shellfish being transferred to cleaning and supports temporarily, utilizes shellfish own metabolism process to excrete pollutants such as the heavy metals in body until reach safety standard.The method spended time is longer, and the foster proportion of goods damageds are higher temporarily, and cause certain pollution to Zan Yang district water quality.By protease, seashells protein digestion is become the small soluble molecules such as amino acid, polypeptide composition, effectively improving the resource utilization of seashells and improve its economic value added, is the necessary ways preparing Marine Products.Because the enzymolysis liquid obtained after enzymolysis is liquid medium, removing advantageously in heavy metal.
Summary of the invention
The object of the present invention is to provide a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal.
The technical solution used in the present invention is:
Effectively remove a method for shellfish protein enzymolysis liquid heavy metal, step is:
1) phytic acid is soluble in water, make the phytic acid aqueous solution;
2) the phytic acid aqueous solution and diatomite are fully mixed into pasty state, heat drying, porphyrize obtains the heavy metal absorbent of tripolite loading phytic acid;
3) in shellfish protein enzymolysis liquid, add the heavy metal absorbent of tripolite loading phytic acid, regulate pH, fully stir, absorption, centrifugal, precipitation is abandoned in filter.
Phytic acid, diatomaceous mass ratio are: 5-50:95-50.
The mass ratio of shellfish protein enzymolysis liquid and heavy metal absorbent is 20 ~ 200: 1.
Adjust ph to 6 ~ 10.
Adsorption temp is 20 ~ 60 DEG C.
Adsorption time is 0.5 ~ 6 hour.
Centrifugal condition is: rotating speed: 2000 ~ 10000R/min; Time: 5 ~ 30 minutes.
Heat drying is micro-wave drying.
The invention has the beneficial effects as follows: method provided by the present invention can reduce the content of beary metal such as arsenic, cadmium, lead, mercury in shellfish enzymolysis liquid simultaneously, meets GB2762-2012 national food safety standard pollutants in food limit standard.Method is simple to operate, cost is low, the reaction time is short, heavy metal complex is easily separated, non-environmental-pollution, product recovery rate are high, is applicable to industrial operation.
Detailed description of the invention
Effectively remove a method for shellfish protein enzymolysis liquid heavy metal, step is:
1) phytic acid is soluble in water, make the phytic acid aqueous solution;
2) the phytic acid aqueous solution and diatomite are fully mixed into pasty state, heat drying, porphyrize obtains the heavy metal absorbent of tripolite loading phytic acid;
3) in shellfish protein enzymolysis liquid, add the heavy metal absorbent of tripolite loading phytic acid, regulate pH, fully stir, absorption, centrifugal, precipitation is abandoned in filter.
Preferably, phytic acid, diatomaceous mass ratio are: 5-50:95-50;
Preferably, heat drying is micro-wave drying.
Preferably, shellfish protein enzymolysis liquid and heavy metal absorbent liquid-solid ratio scope are 20 ~ 200: 1; Preferably, described shellfish is selected from least one in oyster, scallop, mussel, clam;
Preferably, adjust ph to 6 ~ 10.
Preferably, adsorption temp is 20 ~ 60 DEG C; Adsorption time is 0.5 ~ 6 hour.
Preferably, centrifugal actual conditions is: centrifugal 5 ~ 30 minutes of 2000 ~ 10000R/min.
Below in conjunction with embodiment and comparative example, the present invention is described further:
embodiment 1:
In 10L Oyster Protein matter enzymolysis liquid, add the heavy metal absorbent (phytic acid and diatomite mass ratio are 5:95) of 100g tripolite loading phytic acid, adjust ph to 8.0,200rpm/min stirs, and temperature is 45 DEG C, and adsorption time is 1 hour; Centrifugal 10 minutes of 5000R/MIN, precipitation is abandoned in filter.Result is as shown in table 1 below:
table 1 Oyster Protein matter enzymolysis liquid adds tripolite loading phytic acid adsorbent and removes before and after heavy metal and contrast
comparative example 1:
In 10L Oyster Protein matter enzymolysis liquid, add 0.50% phytic acid, adjust ph to 8.0,200rpm/min stirs, and temperature is 45 DEG C, and adsorption time is 1 hour; Centrifugal 10 minutes of 5000R/MIN, precipitation is abandoned in filter.Result is as shown in table 2 below:
table 2 Oyster Protein matter enzymolysis liquid adds phytic acid and removes before and after heavy metal and contrast
comparative example 2:
In 10L Oyster Protein matter enzymolysis liquid, add 100g diatomite, adjust ph to 8.0,200rpm/min stirs, and temperature is 45 DEG C, and adsorption time is 1 hour; Centrifugal 10 minutes of 5000R/MIN, precipitation is abandoned in filter.Result is as shown in table 3 below:
table 3 Oyster Protein matter enzymolysis liquid adds diatomite and removes before and after heavy metal and contrast
Claims (8)
1. effectively remove a method for shellfish protein enzymolysis liquid heavy metal, it is characterized in that: step is:
1) phytic acid is soluble in water, make the phytic acid aqueous solution;
2) the phytic acid aqueous solution and diatomite are fully mixed into pasty state, heat drying, porphyrize obtains the heavy metal absorbent of tripolite loading phytic acid;
3) in shellfish protein enzymolysis liquid, add the heavy metal absorbent of tripolite loading phytic acid, regulate pH, fully stir, absorption, centrifugal, precipitation is abandoned in filter.
2. a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal according to claim 1, is characterized in that: phytic acid, diatomaceous mass ratio are: 5-50:95-50.
3. a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal according to claim 1, is characterized in that: the mass ratio of shellfish protein enzymolysis liquid and heavy metal absorbent is 20 ~ 200: 1.
4. a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal according to claim 1, is characterized in that: adjust ph to 6 ~ 10.
5. a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal according to claim 1, is characterized in that: adsorption temp is 20 ~ 60 DEG C.
6. a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal according to claim 1, is characterized in that: adsorption time is 0.5 ~ 6 hour.
7. a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal according to claim 1, is characterized in that: centrifugal condition is: rotating speed: 2000 ~ 10000R/min; Time: 5 ~ 30 minutes.
8. a kind of method effectively removing shellfish protein enzymolysis liquid heavy metal according to claim 1, is characterized in that: heat drying is micro-wave drying.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110734329A (en) * | 2019-10-10 | 2020-01-31 | 浙江海洋大学 | method for preparing liquid protein fertilizer by using mussel cooking liquor |
| CN112674261A (en) * | 2021-01-04 | 2021-04-20 | 贵州金山蚂蚁保健品有限公司 | Method for reducing heavy metal content of polyrhachis vicina roger |
| CN113854473A (en) * | 2021-09-30 | 2021-12-31 | 无锡定象改性硅胶材料有限公司 | Method for preparing low-arsenic oyster protein peptide by adsorption of targeting nano silica gel material |
| CN114195582A (en) * | 2021-12-27 | 2022-03-18 | 威海长青海洋科技股份有限公司 | Amino acid chelated fertilizer for marine shellfish and preparation method thereof |
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| CN110734329A (en) * | 2019-10-10 | 2020-01-31 | 浙江海洋大学 | method for preparing liquid protein fertilizer by using mussel cooking liquor |
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| CN113854473A (en) * | 2021-09-30 | 2021-12-31 | 无锡定象改性硅胶材料有限公司 | Method for preparing low-arsenic oyster protein peptide by adsorption of targeting nano silica gel material |
| CN113854473B (en) * | 2021-09-30 | 2024-06-04 | 无锡定象改性硅胶材料有限公司 | Method for preparing low-arsenic oyster protein peptide by targeted nano silica gel material adsorption |
| CN114195582A (en) * | 2021-12-27 | 2022-03-18 | 威海长青海洋科技股份有限公司 | Amino acid chelated fertilizer for marine shellfish and preparation method thereof |
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