CN105027873A - Plumbago auriculata shoot cutting propagation method - Google Patents
Plumbago auriculata shoot cutting propagation method Download PDFInfo
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- CN105027873A CN105027873A CN201510335265.1A CN201510335265A CN105027873A CN 105027873 A CN105027873 A CN 105027873A CN 201510335265 A CN201510335265 A CN 201510335265A CN 105027873 A CN105027873 A CN 105027873A
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- cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention provides a plumbago auriculata shoot cutting propagation method. The method comprises the following steps: (1) selecting a cutting substrate; (2) selecting a cutting shoot; (3) treating by a rooting agent; (4) performing the cutting; (5) managing after the cutting. Compared with the prior art, the plumbago auriculata shoot cutting propagation method not only has the advantages of the prior art such as simple conditions, easy operation and low propagation cost, but also can remarkably increase the average main root length of the cut plumbago auriculata, and can greatly improve the survival rate.
Description
Technical field
The present invention relates to a kind of ceratostigma plumbaginoides Bunge softwood cutting propagation method, belong to flowers and trees vegetative propagation technique field.
Background technology
Ceratostigma plumbaginoides Bunge (Plumbagoauriculata) originates in South Africa, has another name called Lan Huadan, Lan Xuedan, blue flower rock pine, blue jasmine etc., belongs to Plumbaginaceae (lumbaginaceae) leadwort perennial evergreen shrub.Ceratostigma plumbaginoides Bunge growing way is strong, heat-resisting, comparatively high-temp resisting high-humidity resisting, and damage by disease and insect is few, and management is simple, and viewing period is long.Leaf color jade green, pattern is simple and elegant, and hot summer gives cooling sensation, potted plantly can intersperse room, balcony; Ripe plant branch pendency, suitable tun combined pot culture, also can be used for afforesting and beautifying of the main section such as venue periphery, road, viaduct, also can plant border plantation or intersperse lawn in ground.The ceratostigma plumbaginoides Bunge florescence on the occasion of few colored inch joint, and is the rare Regulation and Blue Flowers of natural world, is subject to Gardener author's favor.Current ceratostigma plumbaginoides Bunge spends flowers by introducing and planting as a kind of new excellent sight at home, particularly during the 2008 Beijing Olympic Games, being applied in some important greening locations, played good landscape effect, is one of new excellent sight flower flowers decorating Beijing environment during the course of the Olympic Games.
Ceratostigma plumbaginoides Bunge is mainly bred with sowing and cuttage, but seed propagation, and seed Natural seed setting rate is low, not easily obtains seed, and domestic introducing and planting great majority utilize import seeds cultivation, and price is more expensive.Also little to the research of ceratostigma plumbaginoides Bunge softwood cutting propagation technical elements at present, traditional cuttage breeding method rooting rate is lower, and rooting rate is less than 60%, and rootage duration is longer, can not meet the needs of factorial seedling growth.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of ceratostigma plumbaginoides Bunge epicormic branch cutting method.
Technical scheme: for achieving the above object, the invention provides a kind of ceratostigma plumbaginoides Bunge softwood cutting propagation method, comprises the following steps:
(1) cutting medium is selected: adopt turfy soil and perlitic mixed-matrix as cutting medium;
(2) select cuttings: choose the raw ripe ceratostigma plumbaginoides Bunge plant of 4-5 as getting fringe maternal plant, clip as cuttings, prunes cuttings with the edible tender branch of terminal bud, robust growth, semi-lignified;
(3) root-growing agent process: 25-35 second in the solution that step (2) gained cuttings bottom is immersed in containing hormone, described solution concentration is 800-1200mg/L;
(4) cuttage: punch on cutting medium, then cuttings is inserted, compress the matrix of cutting base portion surrounding, shade, regularly water spray keeps humidity;
(5) manage after cuttage: spray water immediately after cuttage 1 time, moisturizing of shading and spray water to be strengthened in 15 days after cuttage, see weather conditions, every day, blade face water spray 2-3 time, kept certain temperature and humidity, in time starting to take root and have young leaves to grow, remove sunshade net gradually, and reducing water spray number of times, every day waters 1 time to matrix, and the cloudy day does not water.
As preferably, in described step (1), turfy soil and perlitic weight ratio are 1:1.
Preferred as another kind, the method that described step (2) prunes cuttings is: cuttings retains 2-3 joint, long 5-8cm, cuttings 2 blades are bottom cut off from the bottom of petiole, cuttings bottom is near joint position straight snips, and cuttings top retains 2-3 sheet tender leaf, and large blade cuts off 1/3-1/2.
Preferred as another kind, the hormone in described step (3) is methyl α-naphthyl acetate, and its solution concentration is 1000mg/L.
Preferred as another kind, the spacing of described step (4) mesopore is 3 × 4cm.
Preferred as another kind, in described step (4), the degree of depth of cuttage is the 1/3-1/2 of cutting length.
Preferred as another kind, temperature 20-25 DEG C in described step (5), humidity is 60-100%.
The present invention is directed to the characteristic of ceratostigma plumbaginoides Bunge, utilize turfy soil and perlitic 1:1 mixing cutting medium, by specifically pruning cuttings method and root-growing agent methyl α-naphthyl acetate 800-1200mg/L, in conjunction with the specific cuttage degree of depth, significantly can increase the average main root length of transplanted seedling, significantly improve survival rate, high-quality ceratostigma plumbaginoides Bunge can be bred effectively in enormous quantities.The inventive method is easy to operation, has that cost is low, technical requirement is simple, the advantage such as easily to apply.
Beneficial effect: relative to prior art, ceratostigma plumbaginoides Bunge softwood cutting propagation method of the present invention, the condition not only with prior art is easy, processing ease, the advantage that reproductive-cost is low, but also the average main length that significantly can increase cuttage ceratostigma plumbaginoides Bunge, and significantly improve survival rate.
Accompanying drawing explanation
Fig. 1: through the ceratostigma plumbaginoides Bunge rooting rate change curve of hormon process
Embodiment
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1
Turfy soil is adopted to mix as cutting medium with perlite 1:1;
Choose the ripe ceratostigma plumbaginoides Bunge plant of life in 4 years as getting fringe maternal plant, with sharp scissors clip with the edible tender branch of terminal bud, robust growth, semi-lignified as cuttings, cuttings retains 2 joints, long 5cm, cuttings 2 blades are bottom cut off from the bottom of petiole, cuttings bottom is near joint position straight snips, and cuttings top retains 2 tender leafs, and larger blade will cut off 1/3.
Cuttings bottom to be immersed in the hormone solution of methyl α-naphthyl acetate (NAA) 800mg/L 25 seconds.
Punch on cutting medium with pencil, spacing keeps 3 × 4cm, then cuttings is inserted, and the degree of depth of cuttage is 1/3 of cutting length, compresses the matrix of cutting base portion surrounding, and cover one deck sunshade net and shade, regularly water spray keeps humidity.
Spray water immediately after cuttage 1 time, will strengthen moisturizing of shading and spray water, see weather conditions after cuttage in 15 days, every day sprays water 2 times in blade face, and keeps temperature to be 20 DEG C, and humidity is 60%.In time starting to take root and have young leaves to grow, will remove sunshade net gradually, and reduce water spray number of times, every day waters 1 time to matrix, and the cloudy day does not water, and is beneficial to ceratostigma plumbaginoides Bunge cuttings new root.
Embodiment 2
Turfy soil is adopted to mix as cutting medium with perlite 1:1;
Choose the ripe ceratostigma plumbaginoides Bunge plant of life in 5 years as getting fringe maternal plant, with sharp scissors clip with the edible tender branch of terminal bud, robust growth, semi-lignified as cuttings, cuttings retains 3 joints, long 8cm, cuttings 2 blades are bottom cut off from the bottom of petiole, cuttings bottom is near joint position straight snips, and cuttings top retains 3 tender leafs, and larger blade will cut off 1/2.
Cuttings bottom to be immersed in the hormone solution of methyl α-naphthyl acetate (NAA) 1200mg/L 35 seconds.
Punch on cutting medium with pencil, spacing keeps 3 × 4cm, then cuttings is inserted, and the degree of depth of cuttage is 1/2 of cutting length, compresses the matrix of cutting base portion surrounding, and cover one deck sunshade net and shade, regularly water spray keeps humidity.
Spray water immediately after cuttage 1 time, will strengthen moisturizing of shading and spray water, see weather conditions after cuttage in 15 days, every day sprays water 3 times in blade face, and keeps temperature to be 25 DEG C, and humidity is 100%.In time starting to take root and have young leaves to grow, will remove sunshade net gradually, and reduce water spray number of times, every day waters 1 time to matrix, and the cloudy day does not water, and is beneficial to ceratostigma plumbaginoides Bunge cuttings new root.
Embodiment 3
Turfy soil is adopted to mix as cutting medium with perlite 1:1;
Choose the ripe ceratostigma plumbaginoides Bunge plant of life in 4 years as getting fringe maternal plant, with sharp scissors clip with the edible tender branch of terminal bud, robust growth, semi-lignified as cuttings, cuttings retains 3 joints, long 7cm, cuttings 2 blades are bottom cut off from the bottom of petiole, cuttings bottom is near joint position straight snips, and cuttings top retains 3 tender leafs, and larger blade will cut off 1/3.
Cuttings bottom to be immersed in the hormone solution of methyl α-naphthyl acetate (NAA) 1000mg/L 30 seconds.
Punch on cutting medium with pencil, spacing keeps 3 × 4cm, then cuttings is inserted, and the degree of depth of cuttage is 1/2 of cutting length, compresses the matrix of cutting base portion surrounding, and cover one deck sunshade net and shade, regularly water spray keeps humidity.
Spray water immediately after cuttage 1 time, will strengthen moisturizing of shading and spray water, see weather conditions after cuttage in 15 days, every day sprays water 2 times in blade face, and keeps temperature to be 22 DEG C, and humidity is 80%.In time starting to take root and have young leaves to grow, will remove sunshade net gradually, and reduce water spray number of times, every day waters 1 time to matrix, and the cloudy day does not water, and is beneficial to ceratostigma plumbaginoides Bunge cuttings new root.
Embodiment 4
Turfy soil is adopted to mix as cutting medium with perlite 1:1;
Choose the ripe ceratostigma plumbaginoides Bunge plant of life in 4 years as getting fringe maternal plant, with sharp scissors clip with the edible tender branch of terminal bud, robust growth, semi-lignified as cuttings, cuttings retains 3 joints, long 5cm, cuttings 2 blades are bottom cut off from the bottom of petiole, cuttings bottom is near joint position straight snips, and cuttings top retains 3 tender leafs, and larger blade will cut off 1/3.
Cuttings bottom to be immersed in the hormone solution of methyl α-naphthyl acetate (NAA) 900mg/L 28 seconds.
Punch on cutting medium with pencil, spacing keeps 3 × 4cm, then cuttings is inserted, and the degree of depth of cuttage is 1/2 of cutting length, compresses the matrix of cutting base portion surrounding, and cover one deck sunshade net and shade, regularly water spray keeps humidity.
Spray water immediately after cuttage 1 time, will strengthen moisturizing of shading and spray water, see weather conditions after cuttage in 15 days, every day sprays water 2 times in blade face, and keeps temperature to be 21 DEG C, and humidity is 70%.In time starting to take root and have young leaves to grow, will remove sunshade net gradually, and reduce water spray number of times, every day waters 1 time to matrix, and the cloudy day does not water, and is beneficial to ceratostigma plumbaginoides Bunge cuttings new root.
Embodiment 5
Turfy soil is adopted to mix as cutting medium with perlite 1:1;
Choose the ripe ceratostigma plumbaginoides Bunge plant of life in 4 years as getting fringe maternal plant, with sharp scissors clip with the edible tender branch of terminal bud, robust growth, semi-lignified as cuttings, cuttings retains 3 joints, long 6cm, cuttings 2 blades are bottom cut off from the bottom of petiole, cuttings bottom is near joint position straight snips, and cuttings top retains 2 tender leafs, and larger blade will cut off 1/3.
Cuttings bottom to be immersed in the hormone solution of methyl α-naphthyl acetate (NAA) 1100mg/L 32 seconds.
Punch on cutting medium with pencil, spacing keeps 3 × 4cm, then cuttings is inserted, and the degree of depth of cuttage is 1/2 of cutting length, compresses the matrix of cutting base portion surrounding, and cover one deck sunshade net and shade, regularly water spray keeps humidity.
Spray water immediately after cuttage 1 time, will strengthen moisturizing of shading and spray water, see weather conditions after cuttage in 15 days, every day sprays water 2 times in blade face, and keeps temperature to be 24 DEG C, and humidity is 90%.In time starting to take root and have young leaves to grow, will remove sunshade net gradually, and reduce water spray number of times, every day waters 1 time to matrix, and the cloudy day does not water, and is beneficial to ceratostigma plumbaginoides Bunge cuttings new root.
The average main root length of experimental example 1 cuttage breeding method of the present invention transplanted seedling and survival rate are investigated
Select the ceratostigma plumbaginoides Bunge cuttings of same batch, stochastic averagina is divided into 3 groups, is respectively embodiment 3 groups, embodiment 4 groups, embodiment 5 groups.
Embodiment 3 groups, embodiment 4 groups and embodiment 5 groups carry out cuttage and seedling culture according to the embodiment of the present invention 3,4 and 5 method respectively.
After transplanting, the management method of each group is all identical with external environment, investigates average main root length and the survival rate of each group, the results are shown in following table 1
Result investigated by the table 1 respectively rear average main root length of group cuttage breeding method transplanting and survival rate
Can be obtained by upper table 1 result, after the embodiment of the present invention 3, embodiment 4 and embodiment 5 propagation method are transplanted, reached at the 6.5cm that the average main root length of ceratostigma plumbaginoides Bunge is the highest, what after transplanting, survival rate was the highest reaches 92.3%, far above 60% of Traditional breeding processes.
The process of experimental example 2 hormon affects test method to ceratostigma plumbaginoides Bunge cuttage root-taking
The growth hormone methyl α-naphthyl acetate (NAA) of experimental study variable concentrations, heteroauxin (IAA), indolebutyric acid (IBA) are on the impact of ceratostigma plumbaginoides Bunge cuttage root-taking, often kind of hormone establishes 500,1000,1500,2000mg/L tetra-concentration gradients, with clear water process in contrast (CK).Test takes randomized complete-block design, each process 30 cuttings, repeats 3 times.Often process cuttings and be 30s in the time of hormone solution, after cuttage 30d, add up the rooting rate of each process, measure take root number and the main root root length of each process by same procedure after 60d, arcsine conversion is carried out to rooting rate numerical value, then utilizes SPSS statistical software to make variance analysis and Multiple range test to transforming numerical, statistical data.
Hormon process affects interpretation of result to ceratostigma plumbaginoides Bunge cuttage root-taking
Under the same terms, compare respectively process ceratostigma plumbaginoides Bunge cuttings with growth hormone NAA, IAA, IBA of variable concentrations and clear water, the rooting rate of each process and number of on average taking root, average main root length are as shown in table 2.
Table 2, hormon process are on the impact of ceratostigma plumbaginoides Bunge cuttage root-taking
As shown in Table 2, three kinds of somatotropin process all can improve ceratostigma plumbaginoides Bunge cutting plantation, and the rooting rate of each concentration process is all higher than clear water control treatment.NAA, IAA, IBA are along with the increase of concentration, and the trend first raising rear reduction all appears in rooting rate, and three kinds of hormones are under the process of variable concentrations condition, and the change curve of rooting rate as shown in Figure 1.
Carry out arcsine conversion to rooting rate, and carry out variance analysis to transforming numerical and SSR Multiple range test is learnt, the rooting rate of each process is all higher than contrast, and difference reaches pole significance level; Difference between the dense plain horizontal processing of difference of same hormone also all reaches pole significance level; The concentration level that NAA HORMONE TREATMENT rooting rate is the highest is 1000mg/L, is the peak in all process, and the concentration level that IAA HORMONE TREATMENT rooting rate is the highest is the concentration level that 1500mg/L, IBA HORMONE TREATMENT rooting rate is the highest is 1500mg/L.
Therefore, for ceratostigma plumbaginoides Bunge, not only its short rooting efficiency is optimum to use NAA root-growing agent, and its consumption also greatly reduces.
Claims (7)
1. a ceratostigma plumbaginoides Bunge softwood cutting propagation method, is characterized in that, comprises the following steps:
(1) cutting medium is selected: adopt turfy soil and perlitic mixed-matrix as cutting medium;
(2) select cuttings: choose the raw ripe ceratostigma plumbaginoides Bunge plant of 4-5 as getting fringe maternal plant, clip as cuttings, prunes cuttings with the edible tender branch of terminal bud, robust growth, semi-lignified;
(3) root-growing agent process: 25-35 second in the solution that step (2) gained cuttings bottom is immersed in containing hormone, described solution concentration is 800-1200mg/L;
(4) cuttage: punch on cutting medium, then cuttings is inserted, compress the matrix of cutting base portion surrounding, shade, regularly water spray keeps humidity;
(5) manage after cuttage: spray water immediately after cuttage 1 time, moisturizing of shading and spray water to be strengthened in 15 days after cuttage, see weather conditions, every day, blade face water spray 2-3 time, kept certain temperature and humidity, in time starting to take root and have young leaves to grow, remove sunshade net gradually, and reducing water spray number of times, every day waters 1 time to matrix, and the cloudy day does not water.
2. ceratostigma plumbaginoides Bunge softwood cutting propagation method according to claim 1, is characterized in that, in described step (1), turfy soil and perlitic weight ratio are 1:1.
3. ceratostigma plumbaginoides Bunge softwood cutting propagation method according to claim 1, it is characterized in that, the method that described step (2) prunes cuttings is: cuttings retains 2-3 joint, long 5-8cm, cuttings 2 blades are bottom cut off from the bottom of petiole, cuttings bottom is near joint position straight snips, and cuttings top retains 2-3 sheet tender leaf, and large blade cuts off 1/3-1/2.
4. ceratostigma plumbaginoides Bunge softwood cutting propagation method according to claim 1, is characterized in that, the hormone in described step (3) is methyl α-naphthyl acetate, and its solution concentration is 1000mg/L.
5. ceratostigma plumbaginoides Bunge softwood cutting propagation method according to claim 1, is characterized in that, the spacing of described step (4) mesopore is 3 × 4cm.
6. ceratostigma plumbaginoides Bunge softwood cutting propagation method according to claim 1, is characterized in that, in described step (4), the degree of depth of cuttage is the 1/3-1/2 of cutting length.
7. ceratostigma plumbaginoides Bunge softwood cutting propagation method according to claim 1, is characterized in that, in described step (5), temperature is 20-25 degree Celsius, and humidity is 60-100%.
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CN107691222A (en) * | 2017-11-10 | 2018-02-16 | 四川农业大学 | A kind of method that indigo plant spends red efficiently cuttage and quick-propagation |
CN108684346A (en) * | 2018-05-03 | 2018-10-23 | 贵阳中医学院 | A kind of cuttage breeding method of Sabia parviflora Wall.ex Roxb |
CN109089577A (en) * | 2018-08-28 | 2018-12-28 | 江苏农林职业技术学院 | A kind of cuttage breeding method of Phnom Penh serissa serissoide |
CN114027040A (en) * | 2021-10-26 | 2022-02-11 | 江苏省农业科学院 | Method for raising seedlings of hydrangea old branches by cutting |
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CN114027040B (en) * | 2021-10-26 | 2023-07-25 | 江苏省农业科学院 | Method for raising seedlings of old branches of hydrangea by cutting |
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