CN105001852A - Preparation method and application of magnetic pyrenebutyric acid fluorescent probe detecting ferric ions - Google Patents
Preparation method and application of magnetic pyrenebutyric acid fluorescent probe detecting ferric ions Download PDFInfo
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- CN105001852A CN105001852A CN201510304316.4A CN201510304316A CN105001852A CN 105001852 A CN105001852 A CN 105001852A CN 201510304316 A CN201510304316 A CN 201510304316A CN 105001852 A CN105001852 A CN 105001852A
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- butyric acid
- pyrene butyric
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- QXYRRCOJHNZVDJ-UHFFFAOYSA-N 4-pyren-1-ylbutanoic acid Chemical compound C1=C2C(CCCC(=O)O)=CC=C(C=C3)C2=C2C3=CC=CC2=C1 QXYRRCOJHNZVDJ-UHFFFAOYSA-N 0.000 title claims abstract description 53
- 239000007850 fluorescent dye Substances 0.000 title claims abstract description 48
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 229910001447 ferric ion Inorganic materials 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- MZMNEDXVUJLQAF-UHFFFAOYSA-N 1-o-tert-butyl 2-o-methyl 4-hydroxypyrrolidine-1,2-dicarboxylate Chemical compound COC(=O)C1CC(O)CN1C(=O)OC(C)(C)C MZMNEDXVUJLQAF-UHFFFAOYSA-N 0.000 claims abstract description 17
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000243 solution Substances 0.000 claims description 32
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 15
- 239000011259 mixed solution Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000005576 amination reaction Methods 0.000 claims description 10
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 10
- 229910052742 iron Inorganic materials 0.000 claims description 8
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Substances CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 claims description 7
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 claims description 7
- -1 iron ion Chemical class 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- HOGDNTQCSIKEEV-UHFFFAOYSA-N n'-hydroxybutanediamide Chemical compound NC(=O)CCC(=O)NO HOGDNTQCSIKEEV-UHFFFAOYSA-N 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000000523 sample Substances 0.000 claims description 6
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- 229940040526 anhydrous sodium acetate Drugs 0.000 claims description 5
- 239000006185 dispersion Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 230000005284 excitation Effects 0.000 claims description 5
- 238000002189 fluorescence spectrum Methods 0.000 claims description 5
- 239000008187 granular material Substances 0.000 claims description 5
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000010907 mechanical stirring Methods 0.000 claims description 5
- 210000002966 serum Anatomy 0.000 claims description 5
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 239000003643 water by type Substances 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 238000003786 synthesis reaction Methods 0.000 claims 2
- 238000001514 detection method Methods 0.000 abstract description 11
- 238000000926 separation method Methods 0.000 abstract description 3
- 230000005389 magnetism Effects 0.000 abstract 2
- 239000008204 material by function Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000005307 ferromagnetism Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010022971 Iron Deficiencies Diseases 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000008575 Pinus pinea Nutrition 0.000 description 1
- 240000007789 Pinus pinea Species 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to a preparation method and application of a magnetic pyrenebutyric acid fluorescent probe detecting ferric ions, and belongs to the technical field of novel functional materials and analytical chemistry. Ferroferric oxide has high magnetism, 1-pyrenebutyric acid has good fluorescent performance, when ferroferric oxide and 1-pyrenebutyric acid are composited, magnetism of ferroferric oxide can be kept, the fluorescent performance of 1-pyrenebutyric acid can be kept as well, and therefore detection and separation of ferric ions can be achieved, the detection limit used for analyzing ferric ions is 0.01 mmol/L, and the linear range ranges from 0.5 mmol/L to 300 mmol/L.
Description
Technical field
The present invention relates to a kind of preparation method and the application that detect the magnetic pyrene butyric acid fluorescent probe of ferric ion, belong to new function material and chemical analysis technology field.
Background technology
Fe
3+be not only a kind of heavy metal ion, and it is also one of most important trace element in human body.It is present in many enzymes and protein, and as the cofactor that cellular metabolism is reacted.Fe
3+blood can promote the formation of oxyphorase, and iron deficiency can cause people's anaemia.But excessive iron level can damage biological system, this is because its redox form catalysis can produce high activity oxygen, and this material can participate in various diseases, as handkerchief parasol pine syndromes, Alzheimer's and cancer.Therefore, in human body, the assay of iron ion is a very important problem.
Fluorometric analysis measures has simple to operate, highly sensitive and efficiency advantages of higher, has now become one of most popular method.
1-pyrene butyric acid is the fluorescence dye of a kind of Fourth Ring aromatics anionic, and this material is used for Fe
3+detection there is not been reported.
Z 250 Fe
3o
4as the nano particle bought, there is ferromagnetism, the steerable performance of magnetic and good biocompatibility, be widely used in multiple fields, as biomagnetism separation, medicament transport release and nuclear magnetic resonance.
Z 250 is connected by 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy-succinamide with 1-pyrene butyric acid by the present invention, and uses it for the detection of ferric ion.The preparation condition of magnetic pyrene butyric acid fluorescent probe is gentle and environmentally friendly.Magnetic pyrene butyric acid fluorescent probe can not only keep the magnetic of Z 250, can also keep the fluorescence property of 1-pyrene butyric acid, can realize the detection to ferric ion and removal simultaneously, thus make magnetic pyrene butyric acid fluorescent probe have good using value.
Summary of the invention
An object of the present invention is a kind of quick, overdelicate magnetic pyrene butyric acid fluorescent probe of preparation.
Two of object of the present invention is the detection and the removal that magnetic pyrene butyric acid fluorescent probe are used for ferric ion.
technical scheme of the present invention is as follows:
1. the preparation of amination Z 250, step is as follows:
The iron trichloride of 1 ~ 3 g is dissolved in 20 ~ 60 mL ethylene glycol, add the anhydrous sodium acetate of 3 ~ 9 g and the anhydrous ethylenediamine of 10 ~ 30 mL, mixed solution mechanical stirring 30 min, mixed solution is transferred in tetrafluoroethylene reactor, 8 h are reacted at 200 DEG C, product through Magneto separate, and cleans 3 ~ 6 times with dehydrated alcohol and water, vacuum-drying 12 h at 60 DEG C.
2. the preparation of magnetic pyrene butyric acid fluorescent probe, step is as follows:
Take 10 ~ 30 mg amination ferroferric oxide nano granules, be scattered in 4 ~ 12 mL ultrapure waters, ultrasonic 30 min, make it to form uniform dispersion liquid; Add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy-succinamide mixing solutions that the 1-pyrene butyric acid of 200 mg and 1 ~ 3 mL molar concentration rate are 1:1, under room temperature, shake 12 h; Under the effect of external magnetic field, unconjugated 1-pyrene butyric acid is carried out being separated and cleans 3 ~ 5 times with distilled water, dry 6 h of product vacuum, obtained magnetic pyrene butyric acid fluorescent probe;
Take 10 ~ 30 mg magnetic pyrene butyric acid fluorescent probes, be scattered in 100 ~ 300 mL, volume ratio be 1: 1 dehydrated alcohol and water mixed solution in, the storing solution of obtained magnetic pyrene butyric acid fluorescent probe, saves backup in 4 DEG C of refrigerators.
3. the application of magnetic pyrene butyric acid fluorescent probe in ferric ion detects, step is as follows:
(1) preparation method according to claim 1 prepares a kind of magnetic pyrene butyric acid fluorescent probe detecting ferric ion;
(2) arrange fluorescent instrument parameter: excitation wavelength is 300 nm, and emission wavelength ranges is 350 ~ 480 nm, excite slit width to be 10 nm, launching slit width is 20 nm, and sweep velocity is 1200 nm/min; The magnetic pyrene butyric acid storing solution of 150 ~ 350 μ L is distributed in the PBS buffered soln of 10 mL, 20 mmol/L, pH 5.0 ~ 8.49, detect the fluorescence spectrum of the ferric ion standardized solution of different concns, according to magnetic pyrene butyric acid fluorescent probe relation between the fluorescence intensity and the concentration of ferric ion at 395 nm places, drawing curve;
(3) human serum sample's solution to be measured is replaced ferric ion standardized solution, detect according to the working curve method for drafting of described iron ion.
useful achievement of the present invention
(1) magnetic ferroferric oxide is incorporated in the preparation of magnetic fluorescence probe, utilizes the advantage such as the steerable performance of the ferromagnetism of magnetic ferroferric oxide, magnetic and good biocompatibility, the separation to target compound can be realized.
(2) utilize the good fluorescence property of 1-pyrene butyric acid, use it for preparation magnetic pyrene butyric acid, the analyzing and testing to target compound can be realized.
(3) amino and carboxyl is activated with 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy-succinamide mixing solutions, the preparation process of the magnetic pyrene butyric acid fluorescent probe made is simple to operation, and environmental sound, process green is clean.
(4) the magnetic pyrene butyric acid fluorescent probe prepared of the present invention not only can Selective recognition ferric ion, iron ion can also be removed fast from solution, the advantages such as have the time of response short, detectability is low, and linearity range is wide simultaneously.Detection for analyzing ferric ion is limited to 0.01 mmol/L, linearity range 0.5 ~ 300 mmol/L.
Embodiment
embodiment 1the preparation of amination Z 250
The iron trichloride of 1 g is dissolved in 20 mL ethylene glycol, add the anhydrous sodium acetate of 3 g and the anhydrous ethylenediamine of 10 mL, mixed solution mechanical stirring 30 min, mixed solution is transferred in tetrafluoroethylene reactor, 8 h are reacted at 200 DEG C, product through Magneto separate, and cleans 3 ~ 6 times with dehydrated alcohol and water, vacuum-drying 12 h at 60 DEG C.
embodiment 2the preparation of amination Z 250
The iron trichloride of 2 g is dissolved in 40 mL ethylene glycol, add the anhydrous sodium acetate of 6 g and the anhydrous ethylenediamine of 20 mL, mixed solution mechanical stirring 30 min, mixed solution is transferred in tetrafluoroethylene reactor, 8 h are reacted at 200 DEG C, product through Magneto separate, and cleans 3 ~ 6 times with dehydrated alcohol and water, vacuum-drying 12 h at 60 DEG C.
embodiment 3the preparation of amination Z 250
The iron trichloride of 3 g is dissolved in 60 mL ethylene glycol, add the anhydrous sodium acetate of 9 g and the anhydrous ethylenediamine of 30 mL, mixed solution mechanical stirring 30 min, mixed solution is transferred in tetrafluoroethylene reactor, 8 h are reacted at 200 DEG C, product through Magneto separate, and cleans 3 ~ 6 times with dehydrated alcohol and water, vacuum-drying 12 h at 60 DEG C;
embodiment 4the preparation of magnetic pyrene butyric acid fluorescent probe
Take 10 amination ferroferric oxide nano granules, be scattered in 4mL ultrapure water, ultrasonic 30 min, make it to form uniform dispersion liquid; Add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy-succinamide mixing solutions that the 1-pyrene butyric acid of 200 mg and 1 mL molar concentration rate are 1:1, under room temperature, shake 12 h; Under the effect of external magnetic field, unconjugated 1-pyrene butyric acid is carried out being separated and cleans 3 ~ 5 times with distilled water, dry 6 h of product vacuum, obtained magnetic pyrene butyric acid fluorescent probe;
Take 10 mg magnetic pyrene butyric acid fluorescent probes, be scattered in 100 mL, volume ratio be 1: 1 dehydrated alcohol and water mixed solution in, the storing solution of obtained magnetic pyrene butyric acid fluorescent probe, saves backup in 4 DEG C of refrigerators.
embodiment 5the preparation of magnetic pyrene butyric acid fluorescent probe
Take 20 mg amination ferroferric oxide nano granules, be scattered in 8 mL ultrapure waters, ultrasonic 30 min, make it to form uniform dispersion liquid; Add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy-succinamide mixing solutions that the 1-pyrene butyric acid of 200 mg and 2 mL molar concentration rates are 1:1, under room temperature, shake 12 h; Under the effect of external magnetic field, unconjugated 1-pyrene butyric acid is carried out being separated and cleans 3 ~ 5 times with distilled water, dry 6 h of product vacuum, obtained magnetic pyrene butyric acid fluorescent probe;
Take 20 mg magnetic pyrene butyric acid fluorescent probes, be scattered in 200 mL, volume ratio be 1: 1 dehydrated alcohol and water mixed solution in, the storing solution of obtained magnetic pyrene butyric acid fluorescent probe, saves backup in 4 DEG C of refrigerators.
embodiment 6the preparation of magnetic pyrene butyric acid fluorescent probe
Take 30 mg amination ferroferric oxide nano granules, be scattered in 12 mL ultrapure waters, ultrasonic 30 min, make it to form uniform dispersion liquid; Add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy-succinamide mixing solutions that the 1-pyrene butyric acid of 200 mg and 3 mL molar concentration rates are 1:1, under room temperature, shake 12 h; Under the effect of external magnetic field, unconjugated 1-pyrene butyric acid is carried out being separated and cleans 3 ~ 5 times with distilled water, dry 6 h of product vacuum, obtained magnetic pyrene butyric acid fluorescent probe;
Take 30 mg magnetic pyrene butyric acid fluorescent probes, be scattered in 300 mL, volume ratio be 1: 1 dehydrated alcohol and water mixed solution in, the storing solution of obtained magnetic pyrene butyric acid fluorescent probe, saves backup in 4 DEG C of refrigerators.
embodiment 7magnetic pyrene butyric acid fluorescent probe is used for the detection of ferric ion, and step is as follows:
(1) preparation method according to claim 1 prepares a kind of magnetic pyrene butyric acid fluorescent probe detecting ferric ion;
(2) arrange fluorescent instrument parameter: excitation wavelength is 300 nm, and emission wavelength ranges is 350 ~ 480 nm, excite slit width to be 10 nm, launching slit width is 20 nm, and sweep velocity is 1200 nm/min.The magnetic pyrene butyric acid storing solution of 150 μ L is distributed in the PBS buffered soln of 10 mL, 20 mmol/L, pH 5.0 ~ 8.49, detect the fluorescence spectrum of the ferric ion standardized solution of different concns, according to magnetic pyrene butyric acid fluorescent probe relation between the fluorescence intensity and the concentration of ferric ion at 395 nm places, drawing curve;
(3) human serum sample's solution to be measured is replaced ferric ion standardized solution, detect according to the working curve method for drafting of described iron ion.Its detection is limited to 0.01 mmol/L, linearity range 0.5 ~ 300 mmol/L.
embodiment 8magnetic pyrene butyric acid fluorescent probe is used for the detection of ferric ion, and step is as follows:
(1) preparation method according to claim 1 prepares a kind of magnetic pyrene butyric acid fluorescent probe detecting ferric ion;
(2) arrange fluorescent instrument parameter: excitation wavelength is 300 nm, and emission wavelength ranges is 350 ~ 480 nm, excite slit width to be 10 nm, launching slit width is 20 nm, and sweep velocity is 1200 nm/min.The magnetic pyrene butyric acid storing solution of 250 μ L is distributed in the PBS buffered soln of 10 mL, 20 mmol/L, pH 5.0 ~ 8.49, detect the fluorescence spectrum of the ferric ion standardized solution of different concns, according to magnetic pyrene butyric acid fluorescent probe relation between the fluorescence intensity and the concentration of ferric ion at 395 nm places, drawing curve;
(3) human serum sample's solution to be measured is replaced ferric ion standardized solution, detect according to the working curve method for drafting of described iron ion.
embodiment 9magnetic pyrene butyric acid fluorescent probe is used for the detection of ferric ion, and step is as follows:
(1) preparation method according to claim 1 prepares a kind of magnetic pyrene butyric acid fluorescent probe detecting ferric ion;
(2) arrange fluorescent instrument parameter: excitation wavelength is 300 nm, and emission wavelength ranges is 350 ~ 480 nm, excite slit width to be 10 nm, launching slit width is 20 nm, and sweep velocity is 1200 nm/min.The magnetic pyrene butyric acid storing solution of 350 μ L is distributed in the PBS buffered soln of 10 mL, 20 mmol/L, pH 5.0 ~ 8.49, detect the fluorescence spectrum of the ferric ion standardized solution of different concns, according to magnetic pyrene butyric acid fluorescent probe relation between the fluorescence intensity and the concentration of ferric ion at 395 nm places, drawing curve;
(3) human serum sample's solution to be measured is replaced ferric ion standardized solution, detect according to the working curve method for drafting of described iron ion.
Claims (2)
1. detect a preparation method for the magnetic pyrene butyric acid fluorescent probe of ferric ion, it is characterized in that, step is as follows:
The synthesis of amination Z 250
The iron trichloride of 1 ~ 3 g is dissolved in 20 ~ 60 mL ethylene glycol, add the anhydrous sodium acetate of 3 ~ 9 g and the anhydrous ethylenediamine of 10 ~ 30 mL, mixed solution mechanical stirring 30 min, mixed solution is transferred in tetrafluoroethylene reactor, 8 h are reacted at 200 DEG C, product through Magneto separate, and cleans 3 ~ 6 times with dehydrated alcohol and water, vacuum-drying 12 h at 60 DEG C;
The synthesis of magnetic pyrene butyric acid fluorescent probe
Take 10 ~ 30 mg amination ferroferric oxide nano granules, be scattered in 4 ~ 12 mL ultrapure waters, ultrasonic 30 min, make it to form uniform dispersion liquid; Add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy-succinamide mixing solutions that the 1-pyrene butyric acid of 200 mg and 1 ~ 3 mL molar concentration rate are 1:1, under room temperature, shake 12 h; Under the effect of external magnetic field, unconjugated 1-pyrene butyric acid is carried out being separated and cleans 3 ~ 5 times with distilled water, dry 6 h of product vacuum, obtained magnetic pyrene butyric acid fluorescent probe;
Take 10 ~ 30 mg magnetic pyrene butyric acid fluorescent probes, be scattered in 100 ~ 300 mL, volume ratio be 1: 1 dehydrated alcohol and water mixed solution in, the storing solution of obtained magnetic pyrene butyric acid fluorescent probe, saves backup in 4 DEG C of refrigerators.
2. a kind of magnetic pyrene butyric acid fluorescent probe detecting ferric ion that prepared by preparation method according to claim 1 detects for ferric ion, and it is characterized in that, step is as follows:
(1) preparation method according to claim 1 prepares a kind of magnetic pyrene butyric acid fluorescent probe detecting ferric ion;
(2) arrange fluorescent instrument parameter: excitation wavelength is 300 nm, and emission wavelength ranges is 350 ~ 480 nm, excite slit width to be 10 nm, launching slit width is 20 nm, and sweep velocity is 1200 nm/min; The magnetic pyrene butyric acid storing solution of 150 ~ 350 μ L is distributed in the PBS buffered soln of 10 mL, 20 mmol/L, pH 5.0 ~ 8.49, detect the fluorescence spectrum of the ferric ion standardized solution of different concns, according to magnetic pyrene butyric acid fluorescent probe relation between the fluorescence intensity and the concentration of ferric ion at 395 nm places, drawing curve;
(3) human serum sample's solution to be measured is replaced ferric ion standardized solution, detect according to the working curve method for drafting of described iron ion.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109439314A (en) * | 2018-12-07 | 2019-03-08 | 武汉工程大学 | The preparation method of the magnetic Nano fluorescence probe of specific recognition Fe (III) |
| CN109900670A (en) * | 2019-04-02 | 2019-06-18 | 济南大学 | A kind of double function probe and its preparation and the application of identification iron ion and fluorine ion |
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| CN102127586A (en) * | 2010-12-08 | 2011-07-20 | 苏州同科生物材料有限公司 | Magnetic fluorescent bifunctional nano biological probe and preparation method thereof |
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| CN103864640A (en) * | 2014-02-27 | 2014-06-18 | 华南师范大学 | N, N '-bis pyrene butyryl ornithine and application thereof |
| CN104045857A (en) * | 2014-06-16 | 2014-09-17 | 安徽师范大学 | Silicon dioxide chitosan composite material, and preparation method and application thereof |
| CN104262811A (en) * | 2014-09-19 | 2015-01-07 | 哈尔滨工业大学 | Preparation method and application of polystyrene fluorescent microspheres |
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Patent Citations (5)
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| CN102127586A (en) * | 2010-12-08 | 2011-07-20 | 苏州同科生物材料有限公司 | Magnetic fluorescent bifunctional nano biological probe and preparation method thereof |
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| CN104045857A (en) * | 2014-06-16 | 2014-09-17 | 安徽师范大学 | Silicon dioxide chitosan composite material, and preparation method and application thereof |
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| CN109439314A (en) * | 2018-12-07 | 2019-03-08 | 武汉工程大学 | The preparation method of the magnetic Nano fluorescence probe of specific recognition Fe (III) |
| CN109439314B (en) * | 2018-12-07 | 2022-05-10 | 武汉工程大学 | Preparation method of magnetic nano fluorescent probe for specifically recognizing Fe (III) |
| CN109900670A (en) * | 2019-04-02 | 2019-06-18 | 济南大学 | A kind of double function probe and its preparation and the application of identification iron ion and fluorine ion |
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