CN104983791A - Health-care product containing coenzyme Q10 and preparation method thereof - Google Patents
Health-care product containing coenzyme Q10 and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of health-care products, in particular to a health-care product containing coenzyme Q10 and a preparation method thereof. The health-care product is a soft capsule which is composed of acapsule shell and filling liquid.The filling liquid comprises, by weight, 30-50 parts of the coenzyme Q10, 20-60 parts of vitamin E, 350-450 parts of perilla seed oil and 20-60 parts of beewax.The capsule shell comprises, by weight, 50-120 parts of gelatin, 50-120 parts of water, 30-60 parts of glycerin and 1-20 parts of pigment. According to the health-care product containing the coenzyme Q10 and the preparation method thereof, the health-care product is prepared from the coenzyme Q10, the perilla seed oil, the vitamin E and the beewax and has the functions of enhancing immunity and protectingheart and cerebral vessels, and the product is stable in quality, convenient to take, safe, effective, free of toxic and side effects, and capable of being takenfor a long time.
Description
Technical field
The present invention relates to health product technology field, be specifically related to a kind of health product containing coenzyme Q10 and preparation method thereof.
Background technology
Modern life rhythm is nervous, and the increasing pressure of family, cause is large, and the emotion of people is also more and more unstable; Simultaneously, excessive consumption of alcohol, the motion taken in too many food fat, lack necessity, in addition the pollution of living environment, the anion concentration in air sharply declines, and the anion taken in body is also just not enough, these factors directly cause human metabolism's speed to slow down, velocity of blood flow can slow down, and blood viscosity raises rapidly, causes cerebral ischemia, if prevent not in time, nurse one's health, the cardiovascular and cerebrovascular diseases such as coronary heart disease, hypertension, cerebral thrombosis, fatty liver will be caused.
Along with the quickening of modern life rhythm; the life stress increasing that common people are general; simultaneously; excessive consumption of alcohol, the motion taken in too many food fat, lack necessity; the a variety of causes such as the pollution grows worse of environment make immune system can not normally play protective effect, in the case, very easily cause the infection such as antibacterial, virus, fungus; therefore hypoimmunity the most directly shows is exactly liable to illness, so modern is badly in need of the immunity strengthening self.Meanwhile, along with the raising of modern life level, traffic is more convenient, causes people easily to take in excess fat, and lack again necessary motion, these easily cause people's blood viscosity to increase simultaneously, if do not noted, cardiovascular and cerebrovascular vessel very easily occurs unexpected.And in current commercially available prod, the product of protection cardiovascular and cerebrovascular vessel while not yet finding to strengthen body immunity, the present invention can strengthen body immunity effectively, effectively protects cardiovascular and cerebrovascular vessel, fills up the blank of commercially available prod simultaneously.
Coenzyme Q10 (also claiming co-ferment Q10) is present in Herba Spinaciae, Brassica oleracea L. var. botrytis L., nut, meat and Fish, is fat soluble nutrient.It has similar structure with vitamin K.Take separately coenzyme Q10 or coordinate vitamin e to take together and can produce very powerful antioxidant.It manufactures in the process of energy (ATP) to do at strengthening health drills very important role.It is dispersed throughout in whole body 5,000 ten thousand to seven 15,000,000 cells, many especially at heart, lungs, liver, kidney, spleen, pancreas and adrenal content.
Coenzyme Q10 is present in the plant in the Nature, and in animal body, human body also can synthesize voluntarily, also can obtain from food.But health is along with the increase of age, the amount of health synthesizing coenzyme Q 10 but reduces, be not enough to reach the standard expecting preventable disease clinically, and coenzyme Q10 also can the constantly consumption when health carries out Repiration, more have research display coenzyme Q10 to lack to be the reason of accelerated ageing.
Also have the nutrient and healthcare products containing coenzyme Q10 at present on the market, but its effect is unsatisfactory.As Chinese patent CN104323283A discloses a kind of alimentation composition containing coenzyme Q10 and preparation method thereof and application, its nutrition composition is made up of according to a certain percentage coenzyme Q10, natural Vitamin E, oil-soluble adjuvant, water soluble adjuvant.Its water soluble adjuvant is selected from least one composition in zinc citrate, sodium selenite, vitamin B1, vitamin B2, vitamin B3, folic acid.Its water soluble adjuvant adopts microencapsulated form to wrap up rear and unclassified stores mixing.In the process making microcapsule, the temperature of fluid bed reaches 60-85 DEG C, and needs fluidisation 0.5-5h, and under this hot conditions, the core of water soluble adjuvant degraded very easily occurs and lost efficacy.And, in the process being pressed into soft capsule, water soluble adjuvant after microencapsulation because of its granule larger, easily blocking nozzle causes production to carry out continuously, larger granule is easily clipped in the crack of soft capsule simultaneously, cause in sizing and dry run and even occur oil leakage phenomenon in shelf life, affect product quality.Its oil-soluble adjuvant and water soluble adjuvant do not have clear superiority in enhancing body immunity simultaneously.
Summary of the invention
The object of this invention is to provide a kind of health product containing coenzyme Q10 and preparation method thereof; this health product coenzyme Q10, perilla oil, vitamin E and Cera Flava are made; there is the function of enhancing immunity and protection cardiovascular and cerebrovascular vessel; constant product quality, taking convenience; safe and effective; have no side effect, can long-term taking.
In order to realize above object, the present invention adopts following technical scheme:
Health product containing coenzyme Q10, described health product comprise following component by weight: coenzyme Q10: 30-50 part, vitamin E: 20-60 part, perilla oil: 350-450 part, Cera Flava: 20-60 part.
Described health product are the soft capsule be made up of rubber and filling liquid, and described filling liquid is prepared from by coenzyme Q10, vitamin E, perilla oil and Cera Flava.
Described rubber comprises following component by weight: gelatin: 50-120 part, water: 50-120 part, glycerol: 30-60 part, pigment: 1-20 part.
Described pigment is titanium dioxide and/or burnt sugar coloring.
Described rubber is made up of following raw material by weight: gelatin: 50-120 part, water: 50-120 part, glycerol: 30-60 part, titanium dioxide 0.1-1 part, burnt sugar coloring 3-10 part.
Described coenzyme Q10 is that fermentation method is obtained and/or semi-synthesis method is obtained and/or complete synthesizing process is obtained and/or biological extraction method is obtained and/or cell culture method obtains.
Described vitamin E is the vitamin E of natural vitamin E and/or synthesis and/or the carboxylate of vitamin E.
A preparation method for the above-mentioned health product containing coenzyme Q10, comprises the following steps:
(1) preparation of colloid solution: take raw material by formula ratio, crosses colloid mill after getting titanium dioxide and part water mix homogeneously, obtains titania solution, and then add burnt sugar coloring mix homogeneously, obtain pigment solution; By glycerol and remaining water mixing post-heating to 60-85 DEG C, drop into gelatin, be stirred to Gelatin, be then evacuated to bubble-free in gelatin solution, then by gelatin solution and pigment solution mix homogeneously and keep colloid solution 55-75 DEG C for subsequent use;
(2) preparation of filling liquid: take raw material by formula ratio, the perilla oil taken is heated to 55-85 DEG C, adds Cera Flava, keep temperature 35-75 DEG C, make the complete melting of Cera Flava, oily wax liquid, when oily wax liquid temperature is down to below 50 DEG C, take coenzyme Q10, vitamin E join in oily wax liquid, be uniformly mixed, cross colloid mill, vacuumize degassing, obtain filling liquid;
(3) pelleting: the filling liquid that the colloid solution obtain step (1) and step (2) obtain suppresses to obtain soft capsule.
Need sizing and dry after described step (3) pelleting, described in be fixed to 10-30 DEG C of cold wind, to soft capsule sizing, described drying is 20-30 DEG C of dry 12-36h.
In described step (1), evacuation is vacuum 0.02-0.15Mpa, time 10-50min.
Coenzyme Q10 has another name called Ubidecarenone, is a kind of fat-soluble quinone, its similar in vitamin K, because of the side chain on its parent nucleus six---the degree of polymerization of polyisoamylene base is 10 and gains the name, and is a kind of quinone cyclics.It is a kind of fat-soluble antioxidant, is one of indispensable important element of human life, the nutrition of energy human activin cell and cellular energy, has functions such as improving body immunity, enhancing antioxidation, slow down aging and enhancing human activity.
Perilla oil take Folium Perillae as the edible oil that raw material extracts, and has significant health care and medical efficacy to human body.In recent years find, perilla oil has the effect of obvious enhancing immunity.Particularly when being in hypoimmunity state, using perilla oil to improve immunity, improving tired state.Folium Perillae about has the history of nearly 2000 in China's plantation application, " book on Chinese herbal medicine justice " cloud: " Folium Perillae, aroma is strong.External-open fur, lets out lung qi and leads to space between skin and muscles; On then stuffy nose relieving plug, refresh oneself is wind and cold diseases caused by exogenous pathogenic factor miraculous cure ".Effect of " Japan hanako materia medica " Yun Qiyou " invigorating the spleen and replenishing QI ", the traditional Chinese medical science commonly uses its disease such as treatment flu of being used as medicine since ancient times.Perilla oil, except strengthening the immunity of human body, has extremely excellent performance in addition in protection human body cardiovascular.Prove through scientific experiments, it is the vegetable oil that in current known plants oils, alpha-linolenic acid content is the highest, enjoys the good name of " bathypelagic fish oil of land ".Linolenic acid abundant in perilla oil not only can control the cohesion of blood in human body in platelet; also there is the effect reducing cholesterol in serum, triglyceride, low density lipoprotein, LDL and very low density lipoprotein (VLDL); thus inhibition thrombosis, prevention myocardial infarction, increase the excretion of various lipid in body; so the effect of perilla oil blood fat-reducing blood pressure-decreasing is obvious especially; especially to hyperlipidemia and borderline hypertension effect more outstanding, effectively protect cardiovascular and cerebrovascular vessel healthy.
Vitamin E is a kind of fatsoluble vitamin, is one of topmost antioxidant.Its antioxidation can protect coenzyme Q10 and perilla oil effectively, avoids effective ingredient oxidized, ensures product efficacy to greatest extent.Vitamin E is not only antioxidant, is again a kind of effective immunomodulator simultaneously, can improves anti-infection ability.Vitimin supplement E can allow the immune system of human body maintain good operation, thus plays the effect improving immunity.In addition, vitamin E can improve lipid metabolism, reduces lipids contents, prevents atherosis, protection cardiovascular and cerebrovascular vessel.
Coenzyme Q10, perilla oil, vitamin E three work in coordination with mutually, triple role, and while enhancing immunity, available protecting cardiovascular and cerebrovascular vessel are healthy.
The present invention also adds Cera Flava as suspending agent, and it is safe and reliable food additive, guarantees whole product quality stable homogeneous in shelf life, and play the effect of this product better in formula.
The health product that the present invention obtains are made up of coenzyme Q10, perilla oil, vitamin E and Cera Flava, have the function of enhancing immunity and protection cardiovascular and cerebrovascular vessel, constant product quality, taking convenience, safe and effective, have no side effect, can long-term taking.
Detailed description of the invention
Embodiment 1
Health product containing coenzyme Q10, be made up of the filling liquid in rubber and rubber, its filling liquid comprises: coenzyme Q10: 45 parts, vitamin E: 20 parts, perilla oil: 415 parts, Cera Flava: 20 parts;
Rubber comprises: 100 parts, gelatin, 100 parts, water, glycerol 40 parts, titanium dioxide 0.2 part, burnt sugar coloring 4 parts.
The described health product its preparation method containing coenzyme Q10 comprises the following steps:
(1) preparation of colloid solution: take needed raw material by above-mentioned formula, crosses colloid mill by after the titanium dioxide that weighs up and partial purification water mix homogeneously, to obtain after titania solution again with burnt sugar coloring mix homogeneously, obtain pigment solution; By glycerol, residue purified water mix homogeneously, then 70-75 DEG C is heated to, drop into gelatin, be stirred to Gelatin complete, then evacuation (vacuum 0.04-0.12Mpa) 25min is to colloid solution bubble-free, then releases colloid solution in storing container, add pigment solution, leave standstill 2 hours after stirring, keep colloid solution 72-75 DEG C, for subsequent use;
(2) preparation of filling liquid: take needed raw material by above-mentioned formula, perilla oil is heated to 80-85 DEG C, add Cera Flava, keep temperature 70-75 DEG C, make the complete melting of Cera Flava, be uniformly mixed, obtain oily wax liquid, for subsequent use, when oily wax liquid temperature is down to 40 DEG C, get coenzyme Q10, vitamin E oil joins in oily wax liquid, be uniformly mixed.Then cross colloid mill, vacuumize degassing, obtain filling liquid;
(3) pelleting: colloid solution and filling liquid are suppressed to obtain soft capsule through automatic rotary soft capsule filling machine, automatically two adhesive tapes are made by pellet press, successively move to rotation film, the flange of the fenestra of left and right transferring film starts to be extruded by adhesive tape, bond, filling liquid is aimed between fenestra quantitative simultaneous implantation two adhesive tape by syringe immediately, because transferring film not stall is moved, the adhesive tape of two-layer parcel feed liquid squeezes off by fenestra flange, boning and being pressed in fenestra forms soft gelatin capsule, and peel off, be separated, obtain soft capsule;
(4) shape: the soft capsule that step (3) is obtained through 20 DEG C of cold air dryings, to soft capsule sizing;
(5) dry: by the soft capsule after sizing 20 DEG C of dry 24h (relative humidity is lower than 35%);
(6) select ball: select conformality ball, remove non-conformality ball, obtain qualified soft gelatin capsule.
Embodiment 2
Health product containing coenzyme Q10, be made up of the filling liquid in rubber and rubber, its filling liquid comprises: coenzyme Q10: 50 parts, vitamin E: 25 parts, perilla oil: 420 parts, Cera Flava: 20 parts;
Rubber comprises: 90 parts, gelatin, 100 parts, water, glycerol 50 parts, titanium dioxide 0.2 part, burnt sugar coloring 4 parts.
The described health product its preparation method containing coenzyme Q10 comprises the following steps:
(1) preparation of colloid solution: take needed raw material by above-mentioned formula, crosses colloid mill by after the titanium dioxide that weighs up and partial purification water mix homogeneously, to obtain after titania solution again with burnt sugar coloring mix homogeneously, obtain pigment solution; By glycerol, residue purified water mix homogeneously, then 60-70 DEG C is heated to, drop into gelatin, be stirred to Gelatin complete, then evacuation (vacuum 0.04-0.12Mpa) 50min is to colloid solution bubble-free, then releases colloid solution in storing container, add pigment solution, leave standstill 2 hours after stirring, keep colloid solution 64-67 DEG C, for subsequent use;
(2) preparation of filling liquid: take needed raw material by above-mentioned formula, perilla oil is heated to 55-60 DEG C, add Cera Flava, keep temperature 35-40 DEG C, make the complete melting of Cera Flava, be uniformly mixed, obtain oily wax liquid, for subsequent use, when oily wax liquid temperature is down to 35 DEG C, get coenzyme Q10, vitamin E oil joins in oily wax liquid, be uniformly mixed.Then cross colloid mill, vacuumize degassing, obtain filling liquid;
(3) pelleting: colloid solution and filling liquid are suppressed to obtain soft capsule through automatic rotary soft capsule filling machine, automatically two adhesive tapes are made by pellet press, successively move to rotation film, the flange of the fenestra of left and right transferring film starts to be extruded by adhesive tape, bond, filling liquid is aimed between fenestra quantitative simultaneous implantation two adhesive tape by syringe immediately, because transferring film not stall is moved, the adhesive tape of two-layer parcel feed liquid squeezes off by fenestra flange, boning and being pressed in fenestra forms soft gelatin capsule, and peel off, be separated, obtain soft capsule;
(4) shape: the soft capsule that step (3) is obtained through 10 DEG C of cold air dryings, to soft capsule sizing;
(5) dry: by the soft capsule after sizing 30 DEG C of dry 24h (relative humidity is lower than 35%);
(6) select ball: select conformality ball, remove non-conformality ball, obtain qualified soft gelatin capsule.
Embodiment 3
Health product containing coenzyme Q10, be made up of the filling liquid in rubber and rubber, its filling liquid comprises: coenzyme Q10: 40 parts, vitamin E: 20 parts, perilla oil: 420 parts, Cera Flava: 20 parts;
Rubber comprises: 110 parts, gelatin, 100 parts, water, glycerol 30 parts, titanium dioxide 0.4 part, burnt sugar coloring 5 parts.
The described health product its preparation method containing coenzyme Q10 comprises the following steps:
(1) preparation of colloid solution: take needed raw material by above-mentioned formula, crosses colloid mill by after the titanium dioxide that weighs up and partial purification water mix homogeneously, to obtain after titania solution again with burnt sugar coloring mix homogeneously, obtain pigment solution; By glycerol, residue purified water mix homogeneously, then 80-85 DEG C is heated to, drop into gelatin, be stirred to Gelatin complete, then evacuation (vacuum 0.04-0.12Mpa) 10min is to colloid solution bubble-free, then releases colloid solution in storing container, add pigment solution, leave standstill 2 hours after stirring, keep colloid solution 55-58 DEG C, for subsequent use;
(2) preparation of filling liquid: take needed raw material by above-mentioned formula, perilla oil is heated to 70-75 DEG C, add Cera Flava, keep temperature 50-55 DEG C, make the complete melting of Cera Flava, be uniformly mixed, obtain oily wax liquid, for subsequent use, when oily wax liquid temperature is down to 30 DEG C, get coenzyme Q10, vitamin E oil joins in oily wax liquid, be uniformly mixed.Then cross colloid mill, vacuumize degassing, obtain filling liquid;
(3) pelleting: colloid solution and filling liquid are suppressed to obtain soft capsule through automatic rotary soft capsule filling machine, automatically two adhesive tapes are made by pellet press, successively move to rotation film, the flange of the fenestra of left and right transferring film starts to be extruded by adhesive tape, bond, filling liquid is aimed between fenestra quantitative simultaneous implantation two adhesive tape by syringe immediately, because transferring film not stall is moved, the adhesive tape of two-layer parcel feed liquid squeezes off by fenestra flange, boning and being pressed in fenestra forms soft gelatin capsule, and peel off, be separated, obtain soft capsule;
(4) shape: the soft capsule that step (3) is obtained through 30 DEG C of cold air dryings, to soft capsule sizing;
(5) dry: by the soft capsule after sizing 25 DEG C of dry 24h (relative humidity is lower than 35%);
(6) select ball: select conformality ball, remove non-conformality ball, obtain qualified soft gelatin capsule.
Embodiment 4
Health product containing coenzyme Q10, be made up of the filling liquid in rubber and rubber, its filling liquid comprises: coenzyme Q10: 30 parts, vitamin E: 40 parts, perilla oil: 450 parts, Cera Flava: 60 parts;
Rubber comprises: 50 parts, gelatin, 80 parts, water, glycerol 60 parts, titanium dioxide 1 part, burnt sugar coloring 8 parts.Its preparation method is with embodiment 1.
Embodiment 5
Health product containing coenzyme Q10, be made up of the filling liquid in rubber and rubber, its filling liquid comprises: coenzyme Q10: 45 parts, vitamin E: 60 parts, perilla oil: 350 parts, Cera Flava: 40 parts; Rubber comprises: 90 parts, gelatin, 120 parts, water, glycerol 30 parts, titanium dioxide 0.7 part, burnt sugar coloring 3 parts.
Its preparation method is with embodiment 2.
Embodiment 6
Health product containing coenzyme Q10, be made up of the filling liquid in rubber and rubber, its filling liquid comprises: coenzyme Q10: 38 parts, vitamin E: 45 parts, perilla oil: 410 parts, Cera Flava: 30 parts; Rubber comprises: 120 parts, gelatin, 50 parts, water, glycerol 45 parts, titanium dioxide 0.5 part, burnt sugar coloring 10 parts.
Its preparation method is with embodiment 3.
Be the functional experiment report of enhancing immunity below
1 materials and methods
1.1 samples: sample is health product prepared by the embodiment of the present invention 1, and specification is 0.5g/ grain, put shady and cool dry place and preserve.(select health product prepared by other embodiments of the invention, its experimental result is roughly the same with the result that embodiment 1 obtains.)
1.2 laboratory animals: the healthy ICR female mice of the SPF level selecting Beijing HFK Bio-Technology Co., Ltd. (production licence number: SCXK (capital) 2009-0004) to breed, body weight 18-23g, totally 192, be divided into four groups, immune one group is carried out internal organs/body weight ratio, delayed allergy, antibody-producting cell detects and serum hemolysin measures; Carbonic clearance experiment is carried out in immunity two groups; Immunity three groups is carried out Turnover of Mouse Peritoneal Macrophages and is engulfed chicken red blood cell experiment; Mouse spleen lymphocyte transformation experiment and the experiment of NK cytoactive detection of ConA induction are carried out in immunity four groups.
1.3 experimental situations: barrier environment, laboratory animal occupancy permit number is SYXK (Shandong) 2008-0005.
1.3 dosage choice:
Sample human body recommended amounts is 0.5g/60kg.bw every day, three dosage groups are established in experiment, that is: 0.042g/kg.bw, 0.083g/kg.bw, 0.250g/kg.bw, the dosage of basic, normal, high three groups is equivalent to 5 times, 10 times, 30 times of human body recommended intake respectively.As solvent, sample is assigned to desired concn respectively using edible peanut oil, namely take 0.17g, 0.33g, 1.00g sample edible peanut oil and be assigned to 40ml respectively, set up solvent control group simultaneously, after 30 days, survey every immune indexes by the continuous per os gavage of 0.1ml/10g.bw every day.
1.4 instruments and reagent
Electronic balance, electronic digital indicator (precision 0.01mm), microsyringe (50 μ l), CO
2incubator, constant water bath box, centrifuge, continuous spectrum microplate reader, microscope, slide frame, 200 eye mesh screens, operating theater instruments, stopwatch, disposable quantitative blood vessel, 24 holes and 96 porocyte culture plates.
SRBC, complement (guinea pig serum), Hanks liquid, SA buffer, agarose, india ink, Na
2cO
3solution, Dou Shi reagent, YAC-1 cell, RPMI1640 complete culture solution, EINECS 212-761-8, INT, PMS, NAD, 0.2mol/LTris-HCl buffer, 1%NP40, ConA, 1% glacial acetic acid, MTT liquid, acid isopropyl alcohol, 1mol/L HCl solution, PBS solution, Giemsa dye liquor.
1.5 experimental techniques:
1.5.1 delayed allergy (DTH) (toes thicken method)
Mouse peritoneal injection 2% (V/V) SRBC, within after sensitization 4 days, measure left back sufficient sole of the foot portion thickness, then be the SRBC (the every Mus of 20 μ l/) of 20% in measuring point subcutaneous injection volume fraction, after injection, 24h measures left back sufficient sole of the foot portion thickness, same position measures 3 times, averages.To inject the difference of the sufficient sole of the foot thickness in front and back, represent the degree of DTH.
Mouse spleen lymphocyte transformation experiment (MTT) method of 1.5.2ConA inducing
Asepticly get spleen, prepare splenocyte suspension, wash 2 times with Hanks liquid, each centrifugal 10min (1000r/min), by cell suspension in 1ml RPMI1640 complete culture solution, adjustment cell concentration is 3 × 10
6individual/ml.Add in 24 well culture plates by a cell suspension point holes, every hole 1ml, a hole adds 75 μ l ConA liquid (being equivalent to 7.5 μ g/ml), and 5%CO in contrast, is put in another hole
2, in 37 DEG C of incubators, cultivate 72h.Cultivation terminates front 4h, every hole Aspirate supernatant 0.7ml, adds 0.7ml not containing the culture fluid of calf serum, adds MTT (5mg/ml) 50 μ l/ hole simultaneously, continues to cultivate 4h.After cultivation terminates, every hole adds 1ml acid isopropyl alcohol, and piping and druming mixing, makes purple crystal dissolve completely.OD value is measured at 570nm wavelength.Deduct with the OD value adding ConA hole the OD value not adding ConA hole and represent lymphocyte proliferation ability.
1.5.3 mice organs/body weight ratio and serum hemolysin measure
SRBC is after five days in mouse peritoneal injection, and pluck eyeball and get blood, centrifuging and taking serum-dilution 150 times, carries out HD50 pH-value determination pH.Then put to death animal, get thymus, spleen is weighed, calculate internal organs/body weight ratio.Prepare splenocyte suspension simultaneously, carry out antibody-producting cell mensuration.
1.5.4 antibody-producting cell detects (Jerne improves slide method)
Get spleen, make cell suspension.Hanks liquid double with equivalent after the culture medium heating for dissolving of top layer is mixed, subpackage small test tube, often pipe 0.5ml, 50 μ l 10%SRBC (v/v, with the preparation of SA liquid), 25 μ l splenocyte suspensions are added again, rapidly after mixing in pipe, be poured on the slide of brush agarose thin layer, after agar solidification, slide level is buckled and is placed on slide frame, put into CO
2incubator cultivates 1.5h, then joins in slide frame groove with the complement (1:8) of SA liquid dilution, after continuing incubation 1.5h, and counting hemolysis plaque number.
1.5.5 mice carbonic clearance is tested
The india ink that mouse tail vein injection 1:3 dilutes, treats that prepared Chinese ink injects timing immediately, injects prepared Chinese ink 2,10min, gets blood 20 μ l respectively, and be added to 2ml Na from angular vein clump
2cO
3in solution, at 600nm wavelength place densitometric value (OD).By sacrifice, get liver and spleen is weighed, calculate phagocytic index.
Phagocytic index a=terminates body weight × K
1/3/ (liver weight+spleen weight) K=(logOD1-logOD2)/(t2-t1)
In above formula, OD1, OD2 are the optical density value of different time institute blood sampling, and t1, t2 are the time difference of getting two blood samples.
1.5.6 Turnover of Mouse Peritoneal Macrophages engulfs chicken red blood cell experiment
The chicken erythrocyte suspension 1ml of mouse peritoneal injection 20%, interval 0.5h puts to death mice, is fixed on Mus plate and cuts off abdominal skin, injecting normal saline 2ml, rotates Mus plate 1min, sucking-off abdominal cavity washing liquid 1ml, divide and drip on 2 slides, 37 DEG C of incubation 30min, use normal saline rinsing, dry, fix with 1:1 acetone methanol solution, Giemsa dye liquor dyes 10 minutes, dries with distilled water rinsing, with oily mirror microscopy, calculate phagocytic rate and phagocytic index.
Phagocytic percentage %=engulfs macrophage number × 100 of the macrophage number/counting of Sanguis Gallus domesticus cell
Phagocytic index=by engulf chicken red blood cell sum/counting macrophage number
1.5.7NK cytoactive detection
Before experiment, 24h is by target cell Secondary Culture, washes 3 times before application with Hanks liquid, is 4 × 10 with RPMI1640 complete culture solution adjustment cell concentration
5individual/ml.Mice cervical dislocation is put to death, and asepticly gets spleen, prepares splenocyte suspension, washes 2 times with Hanks liquid, each centrifugal 10min (1000r/min).Abandon supernatant cytoplasm is upspring, add 0.5ml aquesterilisa splitting erythrocyte, 0.5ml 2 times of Hanks liquid and 8ml Hanks liquid is added again after 20 seconds, centrifugal 10min (1000r/min), resuspended containing the RPMI1640 complete culture solution of calf serum with 1ml, with counting after 1% glacial acetic acid dilution, adjustment cell concentration is 2 × 10
7individual/ml.Target cell is added 96 well culture plates, every hole 100 μ l, experimental port adds 100 μ l splenocytes (effect target is than 50:1), and Spontaneous release hole adds 100 μ l culture fluid, maximum release aperture adds 100 μ l1%NP40, cultivate 4h for 37 DEG C, centrifugal, get supernatant 100 μ l and put in 96 hole ELISA Plate, add LDH matrix liquid 100 μ l, reaction 3min, with the HCl cessation reaction of 1mol/L, measures OD value at microplate reader 490nm place.
NK cytoactive %=(reacting hole OD-Spontaneous release hole OD)/(maximum release aperture OD-Spontaneous release hole OD) × 100
1.6 experimental datas Excel software building database, carries out variance analysis with SPSS software, then carries out statistical analysis with the tournament method (Dunnett method of inspection) of mean between multiple experimental group and a matched group.
2. experimental result
2.1 samples are on the impact of mice delayed allergy
Table 1. sample is on the impact of mice delayed allergy
From table 1, toes swelling and the matched group of basic, normal, high dosage group mice more all have significant difference (P < 0.05, P < 0.01, P < 0.01)
2.2 samples are on the impact of the mouse spleen lymphocyte transformation experiment that ConA induces
Table 2. sample is on the impact of the mouse spleen lymphocyte transformation experiment that ConA induces
From table 2, difference (P > 0.05) that the spleen lymphocyte proliferation ability of basic, normal, high dosage group mice compares with matched group that there are no significant
2.3 samples are on the impact of mice organs/body weight ratio
Table 3. sample is on the impact of mice organs/body weight ratio
From table 3, difference (P > 0.05) that the spleen/body weight ratio of basic, normal, high dosage group mice and thymus/body weight ratio compare with matched group that there are no significant
2.4 samples are on the impact of mouse antibodies cellulation number
Table 4. sample is on the impact of mouse antibodies cellulation number
From table 1, antibody-producting cell number and the matched group of middle and high dosage group mice more all have significant difference (P < 0.01, P < 0.05)
2.5 samples are on the impact of mice serum hemolysin
Table 5. sample is on the impact of mice serum hemolysin
From table 5, difference (P > 0.05) that the serum hemolysin of basic, normal, high dosage group mice compares with matched group that there are no significant
2.6 samples are on the impact of mouse monokaryon-macrophage carbonic clearance ability
Table 6. sample is on the impact of mouse monokaryon-macrophage carbonic clearance ability
From table 6, monocytes/macrophages carbonic clearance phagocytic index and the matched group of low, middle dosage group mice more all have significant difference (P < 0.01, P < 0.05)
2.7 samples engulf the impact of chicken red blood cell ability to mouse peritoneal phagocyte
Table 7. sample engulfs the impact of chicken red blood cell ability to mouse peritoneal phagocyte
From table 7, abdominal cavity phagocytic phagocytic percentage and the matched group of low, middle dosage group mice more all have significant difference (P < 0.05, P < 0.01).Abdominal cavity phagocytic phagocytic index and the matched group of basic, normal, high dosage group mice more all have pole significant difference (P < 0.01)
2.8 samples are on the impact of NK cells in mice activity
Table 8 sample is on the impact of NK cells in mice activity
From table 8, NK cytoactive and the matched group of basic, normal, high dosage group mice more all have pole significant difference (P < 0.01)
2.9 samples are on the impact of Mouse Weight
Table 9 sample is on the impact of Mouse Weight
From table 9, difference (P > 0.05) that the original body mass of mice compares between basic, normal, high dosage group and matched group that there are no significant, namely the original body mass of mice is comparatively balanced between each group.
The body weight in mid-term of mice respectively organized by table 10.
The body weight in latter stage of mice respectively organized by table 11.
Table 12. sample is on the impact of Mouse Weight
From table 10,11,12, the body weight in mid-term of each group mice, latter stage body weight and body weight value added and the difference (P > 0.05) that compares between matched group that there are no significant.
3. brief summary
Experimental result shows, the basic, normal, high dosage group of sample can increase the toes swelling of mice; Middle and high dosage group can improve the antibody-producting cell number of mice; Low, middle dosage group can improve the monocytes/macrophages carbonic clearance ability of mice and peritoneal macrophage engulfs chicken red blood cell percentage rate; Basic, normal, high dosage group can improve the peritoneal macrophage phagocytic index of mice, can strengthen the NK cytoactive of mice.This sample has no significant effect the spleen/body weight ratio of mice and thymus/body weight ratio, the serum hemolysin of mice, the Splenic vein hemodynamics ability of mice.According to " health product inspection and assessment technical specification " criterion, this sample has certain enhancing immunity effect.
Be the functional experiment report of auxiliary antilipemic below
1 materials and methods
1.1 samples: sample is the health product of the embodiment of the present invention 1, and specification is 0.5g/ grain, put shady and cool dry place and preserve.
1.2 laboratory animals: the SD rat selecting Beijing HFK Bio-Technology Co., Ltd. to breed 40, body weight 200 ± 20g, is divided into four groups.
1.3 experimental situations: barrier environment, experimental session ambient temperature is 22 ~ 24 DEG C, relative humidity 52% ~ 58%.
1.3 dosage choice:
Sample human body recommended amounts is 0.5g/60kg.bw every day, three dosage groups are established in experiment, that is: 0.042g/kg.bw, 0.083g/kg.bw, 0.250g/kg.bw, the dosage of basic, normal, high three groups is equivalent to 5 times, 10 times, 30 times of human body recommended intake respectively.As solvent, sample is assigned to desired concn respectively using edible peanut oil, namely take 0.17g, 0.33g, 1.00g sample edible peanut oil and be assigned to 40ml respectively, set up solvent control group simultaneously, by the continuous per os gavage of 0.1ml/10g.bw every day after 30 days, survey indices.
1.4 instruments and reagent
Cholesterol (CHOL), triglyceride (TG), high density lipoprotein (HDL-C) test kit, automatic clinical chemistry analyzer, electronic balance.
High lipid food: normal feedstuff 78.8%, cholesterol 1%, yolk powder 10%, Adeps Sus domestica 10%, cholate 0.2%.
1.5 experimental techniques:
With normal feedstuff feed rat after one week, fasting 16h, get tail blood, measure serum total cholesterol (TC), triglyceride (TG), HDL-C (HDL-C) with automatic clinical chemistry analyzer, take into account TG according to TC level and animal is divided into 4 groups at random: high fat matched group and three tested material groups.From formally testing beginning, each treated animal uses high lipid food instead, and tested material group gives the sample of various dose by above-mentioned dose design gavage, high fat matched group gavage gives the distilled water of same volume, after continuous 30d, fasting 16h, pulls out eyeball blood sampling and measures every blood lipids index.
1.6 experimental datas Excel software building database, carries out variance analysis with SPSS software, then analyzes with multiple experimental group tournament methods of comparable sodium.
2. result
Table 13. sample is on the impact of rat body weight
As shown in Table 13, experimental session, each treated animal growth is normal.Each dosage treated animal original body mass, mid-term body weight, the heavy and hypertrophy of whole opisthosoma compares with high fat matched group, there was no significant difference (p > 0.05).
2.2 samples 1 are on the impact of Serum TC, TG, HDL-C
Table 14. is each group serum TC level before and after testing
From table 14, after experiment, the serum TC level of basic, normal, high dosage group rat more all has significant difference (P < 0.05) with matched group
Table 15. respectively organizes serum TG levels before and after testing
From table 15, after experiment, the serum TG levels of basic, normal, high dosage group rat more all has significant difference (P < 0.05) with matched group
Table 16. is each group Serum HDL-C level before and after testing
From table 16, after experiment, the Serum HDL-C level of basic, normal, high dosage group rat compares there was no significant difference (P > 0.05) with matched group
3. brief summary
Experimental result shows, the basic, normal, high dosage group of sample 1 can reduce serum TC level, the serum TG levels (P < 0.05) of rat, proves that this sample has certain auxiliary antilipemic effect.
Claims (10)
1. the health product containing coenzyme Q10, it is characterized in that, described health product comprise following component by weight: coenzyme Q10: 30-50 part, vitamin E: 20-60 part, perilla oil: 350-450 part, Cera Flava: 20-60 part.
2. the health product containing coenzyme Q10 according to claim 1, it is characterized in that, described health product are the soft capsule be made up of rubber and filling liquid, and described filling liquid is prepared from by coenzyme Q10, vitamin E, perilla oil and Cera Flava.
3. the health product containing coenzyme Q10 according to claim 1, it is characterized in that, described rubber comprises following component by weight: gelatin: 50-120 part, water: 50-120 part, glycerol: 30-60 part, pigment: 1-20 part.
4. the health product containing coenzyme Q10 according to claim 3, it is characterized in that, described pigment is titanium dioxide and/or burnt sugar coloring.
5. the health product containing coenzyme Q10 according to claim 4, it is characterized in that, described rubber is made up of following raw material by weight: gelatin: 50-120 part, water: 50-120 part, glycerol: 30-60 part, titanium dioxide 0.1-1 part, burnt sugar coloring 3-10 part.
6. the health product containing coenzyme Q10 according to claim 1, is characterized in that, described coenzyme Q10 is that fermentation method is obtained and/or semi-synthesis method is obtained and/or complete synthesizing process is obtained and/or biological extraction method is obtained and/or cell culture method obtains.
7. the health product containing coenzyme Q10 according to claim 1, it is characterized in that, described vitamin E is the vitamin E of natural vitamin E and/or synthesis and/or the carboxylate of vitamin E.
8. a preparation method for the health product containing coenzyme Q10 according to claim 1, is characterized in that, comprise the following steps:
(1) preparation of colloid solution: take raw material by formula ratio, crosses colloid mill after getting titanium dioxide and part water mix homogeneously, obtains titania solution, and then add burnt sugar coloring mix homogeneously, obtain pigment solution; By glycerol and remaining water mixing post-heating to 60-85 DEG C, drop into gelatin, be stirred to Gelatin, be then evacuated to bubble-free in gelatin solution, then by gelatin solution and pigment solution mix homogeneously and keep colloid solution 55-75 DEG C for subsequent use;
(2) preparation of filling liquid: take raw material by formula ratio, the perilla oil taken is heated to 55-85 DEG C, adds Cera Flava, keep temperature 35-75 DEG C, make the complete melting of Cera Flava, oily wax liquid, when oily wax liquid temperature is down to below 50 DEG C, take coenzyme Q10, vitamin E join in oily wax liquid, be uniformly mixed, cross colloid mill, vacuumize degassing, obtain filling liquid;
(3) pelleting: the filling liquid that the colloid solution obtain step (1) and step (2) obtain suppresses to obtain soft capsule.
9. the preparation method of the health product containing coenzyme Q10 according to claim 8, it is characterized in that, after described step (3) pelleting, need sizing and dry, described in be fixed to 10-30 DEG C of cold wind, to soft capsule sizing, described drying is 20-30 DEG C of dry 12-36h.
10. the preparation method of the health product containing coenzyme Q10 according to claim 8, is characterized in that, in described step (1), evacuation is vacuum 0.02-0.15Mpa, time 10-50min.
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| CN105831767A (en) * | 2016-03-24 | 2016-08-10 | 袁洪 | Composition for enhancing immunity and resisting fatigue and preparation method and application thereof |
| CN106376918A (en) * | 2016-08-25 | 2017-02-08 | 成都润馨堂药业有限公司 | Health care product preparation for enhancing immunity and preparation method thereof |
| CN106389375A (en) * | 2016-08-18 | 2017-02-15 | 安士生物科技（中山）有限公司 | A coenzyme Q<10> soft capsule preventing oxidation and relieving physical fatigue and a preparing method thereof |
| CN106562937A (en) * | 2016-10-20 | 2017-04-19 | 山东健康源生物工程有限公司 | Coenzyme Q10 soft capsule and preparation method thereof |
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| CN105831767A (en) * | 2016-03-24 | 2016-08-10 | 袁洪 | Composition for enhancing immunity and resisting fatigue and preparation method and application thereof |
| CN106389375A (en) * | 2016-08-18 | 2017-02-15 | 安士生物科技（中山）有限公司 | A coenzyme Q<10> soft capsule preventing oxidation and relieving physical fatigue and a preparing method thereof |
| CN106376918A (en) * | 2016-08-25 | 2017-02-08 | 成都润馨堂药业有限公司 | Health care product preparation for enhancing immunity and preparation method thereof |
| CN106562937A (en) * | 2016-10-20 | 2017-04-19 | 山东健康源生物工程有限公司 | Coenzyme Q10 soft capsule and preparation method thereof |
| CN106902150A (en) * | 2017-03-16 | 2017-06-30 | 桦南仙紫食品科技有限公司 | Co-Q10 purple perilla soft capsule prescription and preparation method |
| CN108236714A (en) * | 2018-01-29 | 2018-07-03 | 江苏神华药业有限公司 | Alleviate physical fatigue and assist the composition and its soft capsule of reducing blood lipid |
| CN108236714B (en) * | 2018-01-29 | 2021-09-10 | 江苏神华药业有限公司 | Composition for relieving physical fatigue and assisting in reducing blood fat and soft capsule thereof |
| CN108295116A (en) * | 2018-02-09 | 2018-07-20 | 南京邦康生物技术有限公司 | A kind of selenium-enriched health care composition, capsule and preparation method thereof |
| CN109007839A (en) * | 2018-08-13 | 2018-12-18 | 王鸿雁 | A kind of health-care edible capsule and preparation method thereof with auxiliary lipid-lowering efficacy |
| CN110585266A (en) * | 2019-09-18 | 2019-12-20 | 广东润和生物科技有限公司 | Application of composition of perilla seed oil and coenzyme Q10 |
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