CN104982516A - Environment-friendly and ecological orange preservation method and preservative - Google Patents
Environment-friendly and ecological orange preservation method and preservative Download PDFInfo
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Abstract
The invention relates to an environment-friendly and ecological orange preservation method and a preservative and belongs to the field of picked fruit and vegetable preservation. According to the method, firstly, fruits for storage are screened; the fruits are soaked in a solution containing polyaminopropyl biguanide for 1-2 minutes to be sterilized; the sterilized oranges are soaked in biological fresh-keeping liquor containing bacillus subtilis R31to be processed for 1-2 minutes; individual packing is conducted on the fruits through polyethylene bags. After oranges are preserved through the method, the purpose of environment-friendly and ecological preservation of oranges can be achieved, the effect equivalent to that of a chemical disinfectant is guaranteed, that is, the rotting rate of oranges is lowered, orange quality is maintained, the length for preservation is prolonged, the orange commodity value is guaranteed, and the market competitiveness of preserved commodities is improved while preservation through the chemical sterilization agent is avoided, residues are reduced, the food safety risk is lowered, consumption of the chemical disinfectant is reduced, influences on the ecology and the environment are reduced, and the method and the preservative are environmentally friendly, ecological and capable of protecting the environment. The method is suitable for industrial production and application and popularization and has good market popularization prospects.
Description
Technical field
The invention belongs to and adopt rear preserving fruit and vegetable utilizing field, relate to citrus fresh-keeping, particularly a kind of green ecological preservation method of citrus and antistaling agent.
Background technology
Citrus is the second largest fruit of China, and output is large, and distribution is wide, and postharvest decay problem is serious, affects citrus industry and develops in a healthy way.Citrus postharvest decay is because pathologic disease and physiological disturbance cause.Physiological disturbance have damage to plants caused by sudden drop in temperature, the disease such as oil vacuole disease, brown spot, oedema and dry scar.Pathologic disease mainly comprises penicilliosis, green mould, black rot, blossom-end rot (comprising the corruption of the brown base of a fruit and the corruption of the black base of a fruit), sour rot and anthracnose etc.
Adopt the fresh-keeping means of rear citrus storage at present, fresh-keeping based on chemical bactericide, Main Ingredients and Appearance have that Prochloraz, imazalil, hundred can obtain, probenazole etc., technology also stays in the chemical sterilization fresh-keeping stage.These compositions itself are also the common drugs adopting the front cultivation of fruit tree phase, use throughout the year, produce resistance, preventive effect declines; Be re-used as post-harvest fresh-keeping medication, residual quantity is a problem; The security of fresh-keeping rear citrus is comparatively difficult to ensure card, there is food security hidden danger; Use waste water impact ecology, the environment of rear discharge.
The fresh-keeping industry of citrus, also has and adds the way that clorox is used as sterilization, as Chinese Patent Application No.: mention in technical scheme disclosed in 201410242839.6 and utilize clorox disinfection at wash phase.Clorox is unstable, sees that light easily decomposes, has chlorine flavor, uses, can affect IAQ in freezer; Have sensitization, often catch tactile, the adverse effects such as palm is perspired in a large number, and nail is thinning, trichomadesis, in disinfecting process, in the environment of the frequent contact sterilizing liquid of human body possibility, clorox is not a good selection.
Polyaminopropyl biguanide is the one of biguanides, molecular formula: (C
5h
14n
6) X, be a kind of fungi-proofing material of mildew-resistant of high-efficiency broad spectrum safety.Material is stablized, the characteristic of cation superpolymer, makes it optionally be adsorbed in pathogenic microorganisms surface, acts on cell membrane, destroys film and eucaryotic cell structure.Research shows that polyaminopropyl biguanide belongs to actual nontoxic level, polyurethane/cationic propyl group biguanides also has purifying water body effect, the polyaminopropyl biguanide of 3-4ppm is perfectly safe to aquatile, environmentally safe, can naturally or by biological decomposition, when can by microbial digestion catabolism after high dilution.Data at home and abroad is reported, polyaminopropyl biguanide is mainly used in the surface sterilization of cosmetics, medicine, food processing, separately has number of patent application 201010542279.8 biguanides to be used for citrus disease control.Disclose the antistaling agent that 0.05% ~ 0.6% hexamethylene and 0.05% ~ 0.2% imidazoles bactericide, benzimidazole germicide are composite, namely composite with imidazoles bactericide, benzimidazole germicide, technology still stays in the chemical sterilization fresh-keeping stage, inevitably there is the fresh-keeping disadvantage of chemical bactericide.
Bacillus subtillis be prevalent in soil, fruits and vegetables and plant surface and in organizing, environmentally safe, nontoxic to man and animal, harmless.More in the relevant report of the feasibility of disease control, Study and appliance about Bacillus subtillis, especially for the research of disease prevention and control in planting process, the Related product of industrialization (" standing grain clear water township (1,000 hundred million spores/gram bacillus subtilis " as Wuhan Tian favour); Fruit post-harvest fresh-keeping application report as the research of Zhan's happiness, Shi Fengyu etc., Yang Zhen etc., Zhao Yan etc., relate generally to Bacillus subtillis to adopt rear fruit disease prevention effect, to the fresh-keeping i.e. quality maintenance effect of fruit, the research of biocontrol mechanism, particularly at the post-harvest fresh-keeping of banana, pears, grape, tomato, indicate Bacillus subtillis and belong to bacterium for adopting the control of rear fruit disease and fresh-keeping feasibility.
Supernatant is gone to obtain wet thallus after the zymotic fluid that Chinese invention patent CN 101870959 B utilizes fermentation of bacillus subtilis to obtain further is centrifugal, wet thallus with etc. the calcium chloride of weight mix, dry to Powdered, obtain dry bacteria, every gram of dry bacteria has 1 × 10
11individual gemma, for black rot of pear, grape grey mould, watermelon blight.Relate to the production of former powder (dry bacteria), but former powder directly uses, and there are the following problems: when (i) diluting, dissolubility is poor in water; (ii) after dilution, bacterium is not protected, and affects comparatively large, as the effect etc. of light, affect the performance of bacterium fresh-keeping effect by external environmental condition; (iii) the poor adherence of former powder, is unfavorable for bacterium powder crop and fruit surface, reduces the effective content of bacterium at crop and fruit surface, reduces fresh-keeping effect.
The comprehensive fresh-keeping industry technology present situation of above citrus storage, loss after effective minimizing Fruit, minimizing chemical sterilization use amount, improve the foodsafety of fresh-keeping fruit, solve medicament residue problem, change the Industry that citrus is fresh-keeping, carry out the exploration of citrus green ecological preservation method.
Therefore, provide a kind of effectively can reduce citrus rotting rate, keep fruit quality and prolong storage period, the citrus disease control of green, safety, environmental protection, the method for preservation and freshness and antistaling agent just become the technical barrier that this technical field is badly in need of solution.
Summary of the invention
First object of the present invention be to provide a kind of effectively can reduce citrus rotting rate, keep fruit quality and prolong storage period, the citrus disease of green, safety, environmental protection is prevented and treated, the method for preservation and freshness.
Foregoing invention object of the present invention reaches by the following technical programs:
A green ecological preservation method for citrus, its step is as follows:
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the citrus fruit rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) disinfect: by citrus fruit stand-by for step (1) gained, be immersed in polyaminopropyl biguanide sterilization listerine process 1-2 minute, to remove fresh citrus fruit remained on surface dirt, surperficial pathogenic microorganisms, to act on the pathogenic bacteria that partly hide, drain for subsequent use;
(3) biological preservation process: the citrus fruit after being disinfected by step (2) gained, is immersed in the biological preservation liquid process 1-2 minute containing bacillus subtilis R31, and process is placed in basket, is placed in sterilization storehouse ventilation airing;
(4) packaging process: the citrus fruit after step (3) process is placed and to be chosen machinery wound after 3-5 days and to rot fruit, and use Polythene Bag individual packing, in suitable condition storage after dress basket, as room temperature storage or refrigeration or ventilation storehouse are preserved.
Polyaminopropyl biguanide described in described step (2) sterilization listerine by Zhuhai Zhenlvse Technology Co., Ltd produce 40 % by weight polyaminopropyl biguanide aqua product, form by 500 times ~ 1000 multiple dilutions.
The concrete formula of the polyaminopropyl biguanide aqua of described 40 % by weight is (weight ratio): polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
The biological preservation liquid containing bacillus subtilis R31 described in described step (3), by the citrus bio-preservative containing bacillus subtilis R31, forms by 200 times ~ 800 times multiple dilutions.
Second goal of the invention of the present invention is to provide a kind of citrus bio-preservative containing bacillus subtilis R31.
Foregoing invention object of the present invention reaches by the following technical programs:
1, the preparation method of the former powder of bacillus subtilis R31, comprises the steps:
(1) activated spawn
Activation bacillus subtilis R31 bacterial strain (bacillus subtilis R31, it is characterized in that " broad-spectrum antifungal plant endophytic bacillus subtillis described in patent CN 101760438B and application thereof " Patent bacterial strain " bacillus subtilis R31 "), treat the bacterium colony milky of bacillus subtilis R31 bacterial strain on NA solid medium, the tiny circle of bacterium colony, smooth surface thickness, without obviously protruding, partial folds;
(2) preparation of primary seed solution
With NA fluid nutrient medium as primary-seed medium, choose the single bacterium colony on NA solid medium, be inoculated in first order seed shaking flask, by first order seed shaking flask 35 DEG C, cultivate 9 hours under 200r/min, obtain primary seed solution;
(3) preparation of secondary seed solution
Culture medium in seeding tank is still NA fluid nutrient medium, and at 121 DEG C, sterilizing 20min, accesses primary seed solution in seeding tank and cultivate, obtain secondary seed solution at the end of cultivation;
(4) bacillus subtilis R31 ferments
By the secondary seed solution of step (3) gained bacillus subtilis R31, be in 1% access fermentation tank according to inoculum concentration, cultivate under fermentation medium, obtain zymocyte liquid (being mainly gemma);
(5) the former powder of bacillus subtilis R31 bacterium is produced
The zymocyte liquid of step (4) gained is after disk plate centrifuge is concentrated, add precipitated calcium carbonate, the weight ratio of concentrated fermenting liquid and precipitated calcium carbonate is made to be 100:3, obtain liquid-solid mixture, after this liquid-solid mixture is stirred 35min, utilize dehvery pump to be sent into by liquid-solid mixture in atomizer tower dry, obtain the former powder of bacillus subtilis R31 bacterium.
NA culture medium prescription (g/L) described in described step (1): peptone 5g, beef extract 3g, glucose 2.5g, agar 15g, solvent is water, and 1000mL, pH7.2 do not add 1.5% agar in fluid nutrient medium;
The inoculum concentration of seeding tank described in described step (3) is 1% (V/V); Condition of culture is: ventilation ratio 1:1, and speed of agitator is 200r/min, cultivation temperature 35 DEG C, tank pressure 0.05Mpa, cultivates 11 hours; The viable bacteria content of described secondary seed solution is 2-6 × 10
8cfu/mL, OD value is greater than 0.85;
The formula (percentage by weight) of fermentation medium described in described step (4): cornstarch 2%, bean cake powder 1.5%, yeast extract 0.8%, sodium chloride 0.25%, magnesium sulfate 0.4%, dipotassium hydrogen phosphate 0.3%, pH7.2,121 DEG C of sterilizing 30min; Condition of culture is: ventilation ratio is 1:0.7, and speed of agitator is 200r/min, tank pressure 0.05MPa, cultivation temperature 35 DEG C, incubation time are 48h, adds defoamer in sweat according to foam situation; The lower tank time will be as the criterion to sample microscopy gemma more than 90% release, and in zymotic fluid, the living spores content of the former powder of bacillus subtilis R31 bacterium is 40 ~ 60 × 10
8cfu/mL;
Described in described step (5), the drying condition of atomizer tower is: intake air temperature is 180-200 DEG C, and air outlet temperature is 80-85 DEG C, and in the former powder of bacterium, water content is less than 5% (W/W), and living spores content is greater than 2000 × 10
8cfu/g.
2, containing a citrus bio-preservative of bacillus subtilis R31, its product forms is wettable powder or oil-suspending agent.
Containing a preparation for the citrus bio-preservative wettable powder of bacillus subtilis R31, its step is as follows:
(1) the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide, wetting agent, dispersant, a certain amount of carrier, co-grinding 1.5 hours in high speed Universalpulverizer;
(2) on step (1) basis, add stabilizing agent, and supply carrier, continue pulverizing 1 hour, must containing the wettable powder of bacillus subtilis R31 living spores.
Gained containing the component of citrus bio-preservative wettable powder of bacillus subtilis R31 and proportioning as follows:
Described wetting agent, dispersant, stabilizing agent, carrier are conventional surfactant.
Described wetting agent is AEO, pulls open the mixing of one or more arbitrary proportions in powder, lauryl alcohol polyethylene glycol oxide base ether sodium sulfate, neopelex, calcium dodecyl benzene sulfonate, sldium lauryl sulfate and ethoxylated dodecyl alcohol sodium sulfonate.
Described dispersant is the mixing of one or more arbitrary proportions in dispersing agent MF, lignosulfonates, naphthalenesulfonic acid-formaldehyde condensate (NN0), polyvinylpyrrolidone, polyethylene glycol and polyvinyl alcohol.
Described stabilizing agent is the mixing of one or more arbitrary proportions in sucrose, sodium chloride, potassium sorbate and ascorbic acid.
Described carrier is as the mixing of one or more arbitrary proportions in precipitated calcium carbonate, kaolin and diatomite.
Containing a preparation for the citrus bio-preservative oil-suspending agent of bacillus subtilis R31, its step is as follows:
(1) the quantitative former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide, Component Vectors, emulsifying agent, mix and blend 2 hours in mixing kettle;
(2) add stabilizing agent in the mixing kettle after stirring 2 hours toward step (1), and supply carrier to 100%, continue stirring 0.5 hour, the stable oil-suspending agent containing bacillus subtilis R31 living spores can be formed.
Gained containing the component of citrus bio-preservative oil-suspending agent of bacillus subtilis R31 and proportioning as follows:
Described floating agent stabilizing agent, carrier are conventional surfactant or chemical composition.
Described emulsifying agent is the mixing of one or more arbitrary proportions in polyoxyethylene sorbitol acid anhydride monopalmitate (polysorbate40), polyoxyethylene sorbitan monostearate (polysorbate60), Tween-80 (Tween 80), AEO, Brij and cithrol.
Described stabilizing agent is the mixing of one or more arbitrary proportions in potassium sorbate, Sodium Benzoate, sodium chloride, sucrose and sodium acetate.
Described carrier is as the mixing of one or more arbitrary proportions of soybean oil, rapeseed oil, epoxidized soybean oil and corn wet goods.
The present invention has following advantages and effect:
The present invention is a kind of citrus green, ecological preservation method, sterilization used, bio-preservative are all actual nontoxic materials,, safety nontoxic to people, animal and environment, after guarantee is adopted citrus, overall fresh-keeping effect simultaneously, improve the foodsafety of fresh-keeping fruit, solve medicament residue problem, reduce chemical sterilization medicament and use, change the Industry that citrus is fresh-keeping; Reduce waste water to impact that is ecological, environment, the water after process also helps soil disease control, purifies water, free from environmental pollution, environmental protection, ecology; Guarantee that citrus green ecological preservation method of the present invention reaches the object of food security, environmental friendliness, green ecological.
The preservation method that citrus of the present invention is green, ecological, tool obvious characteristic: certain density polyaminopropyl biguanide is to citrus storage disease tool broad-spectrum antibacterial, can effectively remove fruit surface microorganism, the microorganism of effects on surface and wound plays detoxicating, degermation, and can partial action in the germ of contaminating that hides, reduce the generation of disease, but more weak to physiology quality regulating and controlling effect, the easy deterioration of anaphase storage physiology, Disease management is not good enough.Bacillus subtilis R31 is the existing patented strain (patent No.: 200910214430.2), the bacterial strain of tool broad-spectrum disease resistance fungal pathogens activity, experiment indicates validity citrus being infected to disease, the induced resistance of bacterium and secondary metabolite can effectively regulate citrus post-harvest physiology, improve fruit disease resistance, delay physiological aging, keep storage quality, ensure storage and shelf life, but biological anti-microbial inoculum prevention effect need further lifting, affects greatly by storage environment.
The inventive method makes full use of complementation between the two, utilizes sterilization farthest to be removed by most of germ, and recycling bacteria agent controls remaining pathogenic bacteria, induced resistance, physiological regulatory action, improve fruit disease resistance, delay physiological aging, keep storage quality.Utilize the effect of biological bacteria secondary metabolites and bacterium itself to degrade a small amount of polyaminopropyl biguanide simultaneously, reduce residual in fruit face of chemical substance.Therefore utilize the inventive method Citrus Fruits In Storage can effectively reduce citrus rotting rate, keep fruit quality prolonging storage period, ensure fruit commodity value, the safety of food, green character, improve fresh-keeping commodity market competitiveness.
Below by detailed description of the invention, the present invention will be further described, but and do not mean that limiting the scope of the invention.
Detailed description of the invention
Reference examples 1
For Germicidal efficacy holding conditions is based on normal temperature: 20-25 DEG C, preserve under relative humidity (RH) 75-90% condition.
Select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by.
(1) Preservation Treatment: by stand-by granulated sugar tangerine, is immersed in 500 times of dilute solution process 1min of 25% Prochloraz, is positioned over the vacant room after sterilization, ventilation of windowing after process.
(2) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(3) result: in table 1.
25% described Prochloraz missible oil, is produced by Zhuhai Zhenlvse Technology Co., Ltd.
Embodiment 1
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, be immersed in 500 times of dilute solution process 2 minutes of 40 % by weight polyaminopropyl biguanides, remove fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, act on the pathogenic bacteria that partly hide, drain for subsequent use;
(3) biological preservation process: by the granulated sugar tangerine after disinfecting, is immersed in 200 times of dilute solution process 1 minute of 70% bacillus subtilis R31 bio-preservative, is positioned over the vacant room after sterilization, ventilation of windowing after process;
(4) packaging process: choose the rotten fruit of machinery wound after 3 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(5) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
The citrus bio-preservative of 70% described bacillus subtilis R31, is every gram and contains 1.4 × 10
11the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
1, the preparation method of the former powder of bacillus subtilis R31, comprises the steps:
(1), activated spawn
Activation bacillus subtilis R31 bacterial strain (bacillus subtilis R31, it is characterized in that " broad-spectrum antifungal plant endophytic bacillus subtillis described in patent CN 101760438B and application thereof " Patent bacterial strain " bacillus subtilis R31 "), treat the bacterium colony milky of bacillus subtilis R31 bacterial strain on NA solid medium, the tiny circle of bacterium colony, smooth surface thickness, without obviously protruding, partial folds;
(2), the preparation of primary seed solution
With NA fluid nutrient medium as primary-seed medium, choose the single bacterium colony on NA solid medium, be inoculated in first order seed shaking flask, by first order seed shaking flask 35 DEG C, cultivate 9 hours under 200r/min, obtain primary seed solution;
(3), the preparation of secondary seed solution
Culture medium in seeding tank is still NA fluid nutrient medium, and at 121 DEG C, sterilizing 20min, accesses primary seed solution in seeding tank and cultivate, obtain secondary seed solution at the end of cultivation;
(4), bacillus subtilis R31 ferments
By the secondary seed solution of step (3) gained bacillus subtilis R31, be in 1% access fermentation tank according to inoculum concentration, cultivate under fermentation medium, obtain zymocyte liquid (being mainly gemma);
(5), the former powder of bacillus subtilis R31 bacterium is produced
The zymocyte liquid of step (4) gained is after disk plate centrifuge is concentrated, add precipitated calcium carbonate, the weight ratio of concentrated fermenting liquid and precipitated calcium carbonate is made to be 100:3, obtain liquid-solid mixture, after this liquid-solid mixture is stirred 35min, utilize dehvery pump to be sent into by liquid-solid mixture in atomizer tower dry, obtain the former powder of bacillus subtilis R31 bacterium.
NA culture medium prescription (g/L) described in described step (1): peptone 5g, beef extract 3g, glucose 2.5g, agar 15g, solvent is water, and 1000mL, pH7.2 do not add 1.5% agar in fluid nutrient medium;
The inoculum concentration of seeding tank described in described step (3) is 1% (V/V); Condition of culture is: ventilation ratio 1:1, and speed of agitator is 200r/min, cultivation temperature 35 DEG C, tank pressure 0.05Mpa, cultivates 11 hours; The viable bacteria content of described secondary seed solution is 2-6 × 10
8cfu/mL, OD value is greater than 0.85;
The formula (percentage by weight) of fermentation medium described in described step (4): cornstarch 2%, bean cake powder 1.5%, yeast extract 0.8%, sodium chloride 0.25%, magnesium sulfate 0.4%, dipotassium hydrogen phosphate 0.3%, pH7.2,121 DEG C of sterilizing 30min; Condition of culture is: ventilation ratio is 1:0.7, and speed of agitator is 200r/min, tank pressure 0.05MPa, cultivation temperature 35 DEG C, incubation time are 48h, adds defoamer in sweat according to foam situation; The lower tank time will be as the criterion to sample microscopy gemma more than 90% release, and in zymotic fluid, the living spores content of the former powder of bacillus subtilis R31 bacterium is 40 ~ 60 × 10
8cfu/mL;
Described in described step (5), the drying condition of atomizer tower is: intake air temperature is 180-200 DEG C, and air outlet temperature is 80-85 DEG C, and in the former powder of bacterium, water content is less than 5% (W/W), and living spores content is greater than 2000 × 10
8cfu/g.
Containing a preparation for the citrus bio-preservative wettable powder of bacillus subtilis R31, its step is as follows:
(1) the former powder of bacillus subtilis R31 is taken in proportion, chitosan oligosaccharide, as calcium dodecyl benzene sulfonate, lignosulfonates, diffusant (MF), co-grinding 1.5 hours in high speed Universalpulverizer;
(2) on step (1) basis, add sucrose, ascorbic acid, and supply precipitated calcium carbonate, continue pulverizing 1 hour, must containing the Microbial wettable powder of bacillus subtilis R31 living spores.
Its composition and proportioning as follows:
(1) 70 grams, the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide 10 grams, calcium dodecyl benzene sulfonate 2 grams, lignosulfonates 2.5 grams, dispersing agent MF 2 grams, co-grinding 1.5 hours in high speed Universalpulverizer;
(2) on step (1) basis, add sucrose 1 gram, 2 grams, ascorbic acid, and supply precipitated calcium carbonate 10.5 grams, continue pulverizing 1 hour, must containing the Microbial wettable powder of bacillus subtilis R31 living spores.
Reference examples 2
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, be immersed in 500 times of dilute solution process 2 minutes of 40 % by weight polyaminopropyl biguanides, remove fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, act on the pathogenic bacteria that partly hide, drain, ventilate;
(3) packaging process: choose the rotten fruit of machinery wound after 3 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
Reference examples 3
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) biological preservation process: by stand-by granulated sugar tangerine, is immersed in 200 times of dilute solution process 1 minute of 70% bacillus subtilis R31 antistaling agent, is positioned over the vacant room after sterilization, ventilation of windowing after process;
(3) packaging process: choose the rotten fruit of machinery wound after 3 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The citrus bio-preservative of 70% described bacillus subtilis R31, is every gram and contains 1.4 × 10
11the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
Reach particular by following technical scheme: for gross weight 100 grams, its composition and proportioning as follows:
(1) 70 grams, the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide 10 grams, calcium dodecyl benzene sulfonate 2 grams, lignosulfonates 2.5 grams, dispersing agent MF 2 grams, co-grinding 1.5 hours in high speed Universalpulverizer;
(2) on step (1) basis, add sucrose 1 gram, 2 grams, ascorbic acid, and supply precipitated calcium carbonate 10.5 grams, continue pulverizing 1 hour, must containing the Microbial wettable powder of bacillus subtilis R31 living spores.
Embodiment 2
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, be immersed in 1000 times of dilute solution process 1-2 minute of 40% polyaminopropyl biguanide, remove fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, act on the pathogenic bacteria that partly hide, drain for subsequent use;
(3) biological preservation process: by the granulated sugar tangerine after disinfecting, is immersed in 1000 times of dilute solution process 1 minute of 10% bacillus subtilis R31 bio-preservative, is positioned over the vacant room after sterilization, ventilation of windowing after process;
(4) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(5) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
The citrus bio-preservative of 10% described bacillus subtilis R31, is every gram and contains 2 × 10
10the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
The formula of the citrus bio-preservative of 10% bacillus subtilis R31 of the present invention is as follows:
10 grams, the former powder of bacillus subtilis R31 (preparation method is identical with embodiment 1)
(1) take 10 grams, the former powder of bacillus subtilis R31, chitosan oligosaccharide 10 grams, AEO 4 grams, pull open 2 grams, powder, polyvinylpyrrolidone 3 grams, co-grinding 1.5 hours in high speed Universalpulverizer;
(2) on step (1) basis, add 1 gram, sodium chloride, and supply precipitated calcium carbonate 70 grams, continue pulverizing 1 hour, must containing the Microbial wettable powder of bacillus subtilis R31 living spores.
Reference examples 4
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, be immersed in 1000 times of dilute solution process 1-2min of 40% polyaminopropyl biguanide, remove fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, act on the pathogenic bacteria that partly hide, drain, ventilate;
(3) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
Reference examples 5
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) biological preservation process: by stand-by granulated sugar tangerine, is immersed in 1000 times of dilute solution process 1min of 10% bacillus subtilis R31 bio-preservative, is positioned over the vacant room after sterilization, ventilation of windowing after process;
(3) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The citrus bio-preservative of 10% described bacillus subtilis R31, is every gram and contains 2 × 10
10the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
The formula of the citrus bio-preservative of 10% bacillus subtilis R31 of the present invention is as follows:
(1) take 10 grams, the former powder of bacillus subtilis R31, chitosan oligosaccharide 70 grams, AEO 4 grams, pull open 2 grams, powder, polyvinylpyrrolidone 3 grams, co-grinding 1.5 hours in high speed Universalpulverizer;
(2) on step (1) basis, add 1 gram, sodium chloride, and supply precipitated calcium carbonate 10 grams, continue pulverizing 1 hour, must containing the Microbial wettable powder of bacillus subtilis R31 living spores.
Embodiment 3
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, is immersed in 800 times of dilute solution process 1min of 40% polyaminopropyl biguanide, removes fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, acts on the pathogenic bacteria that partly hide, drain for subsequent use;
(3) biological preservation process: by the granulated sugar tangerine after disinfecting, be immersed in 800 times of dilute solution process 1min of 50% bacillus subtilis R31 bio-preservative, be positioned over the vacant room after sterilization after process, ventilation of windowing;
(4) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(5) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
The citrus bio-preservative of 50% described bacillus subtilis R31, is every gram and contains 1 × 10
11the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
Composition and the proportioning of the citrus bio-preservative of 50% bacillus subtilis R31 of the present invention are as follows:
(1) 50 grams, the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide 25 grams, neopelex 4 grams, NN01 gram, lignosulfonates 2 grams, co-grinding 2 hours in high speed Universalpulverizer;
(2) on step (1) basis, add potassium sorbate 1 gram, and supply kaolin 16.5 grams, continue pulverizing 1 hour, must containing the Microbial wettable powder of bacillus subtilis R31 living spores.
Reference examples 6
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, is immersed in 800 times of dilute solution process 1min of 40% polyaminopropyl biguanide, removes fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, acts on the pathogenic bacteria that partly hide, drain, ventilate;
(3) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
Reference examples 7
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) biological preservation process: by stand-by granulated sugar tangerine, is immersed in 800 times of dilute solution process 1min of 25% bacillus subtilis R31 antistaling agent, is positioned over the vacant room after sterilization, ventilation of windowing after process;
(3) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The citrus bio-preservative of 50% described bacillus subtilis R31, is every gram and contains 1 × 10
11the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
Composition and the proportioning of the citrus bio-preservative of 50% bacillus subtilis R31 of the present invention are as follows:
(1) 50 grams, the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide 25 grams, neopelex 4 grams, NN01 gram, lignosulfonates 2 grams, co-grinding 2 hours in high speed Universalpulverizer;
(2) on step (1) basis, add potassium sorbate 1 gram, and supply kaolin 16.5 grams, continue pulverizing 1 hour, must containing the Microbial wettable powder of bacillus subtilis R31 living spores.
Embodiment 4
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, is immersed in 600 times of dilute solution process 1min of 40% polyaminopropyl biguanide, removes fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, acts on the pathogenic bacteria that partly hide, drain for subsequent use;
(3) biological preservation process: by the granulated sugar tangerine after disinfecting, be immersed in 600 times of dilute solution process 1min of 30% bacillus subtilis R31 bio-preservative, be positioned over the vacant room after sterilization after process, ventilation of windowing;
(4) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(5) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
The citrus bio-preservative of 30% described bacillus subtilis R31, is every gram and contains 6 × 10
10the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
Composition and the proportioning of the citrus bio-preservative of 30% bacillus subtilis R31 of the present invention are as follows:
(1) 30 grams, the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide 15 grams, soybean oil 20 grams, AEO 12 grams, cithrol 8 grams, mix and blend 2 hours in mixing kettle;
(2) Sodium Benzoate 1 gram is added in the mixing kettle after stirring 2 hours toward step (1), 3 grams, sodium chloride, sucrose 0.5 gram, and supply soybean oil to 100%, continue stirring 0.5 hour, the stable oil-suspending agent containing bacillus subtilis R31 living spores can be formed.
Reference examples 8
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, is immersed in 600 times of dilute solution process 1min of 40% polyaminopropyl biguanide, removes fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, acts on the pathogenic bacteria that partly hide, drain, ventilate;
(3) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
Reference examples 9
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) biological preservation process: by stand-by granulated sugar tangerine, is immersed in 600 times of dilute solution process 1min of 30% bacillus subtilis R31 antistaling agent, is positioned over the vacant room after sterilization, ventilation of windowing after process;
(3) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The citrus bio-preservative of 30% described bacillus subtilis R31, is every gram and contains 6 × 10
10the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
Composition and the proportioning of the citrus bio-preservative of 30% bacillus subtilis R31 of the present invention are as follows:
(1) 30 grams, the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide 15 grams, soybean oil 20 grams, AEO 12 grams, cithrol 8 grams, mix and blend 2 hours in mixing kettle;
(2) Sodium Benzoate 1 gram is added in the mixing kettle after stirring 2 hours toward step (1), 3 grams, sodium chloride, sucrose 0.5 gram, and supply soybean oil to 100%, continue stirring 0.5 hour, the stable oil-suspending agent containing bacillus subtilis R31 living spores can be formed.
Embodiment 5
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, is immersed in 1000 times of dilute solution process 1min of 40% polyaminopropyl biguanide, removes fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, acts on the pathogenic bacteria that partly hide, drain for subsequent use;
(3) biological preservation process: by the granulated sugar tangerine after disinfecting, be immersed in 300 times of dilute solution process 2min of 15% bacillus subtilis R31 bio-preservative, be positioned over the vacant room after sterilization after process, ventilation of windowing;
(4) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(5) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
The citrus bio-preservative of 15% described bacillus subtilis R31, is every gram and contains 3 × 10
10the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
Composition and the proportioning of the citrus bio-preservative of the present invention 15% bacillus subtilis R31 are as follows:
(1) 15 grams, the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide 30 grams, rapeseed oil 20 grams, Brij 9 grams, Tween-80 (Tween 80) 10 grams, mix and blend 2 hours in mixing kettle;
(2) potassium sorbate 1.5 grams is added in the mixing kettle after stirring 2 hours toward step (1), sodium acetate 2 grams, sucrose 1 gram, and supply rapeseed oil to 100%, continue stirring 0.5 hour, the stable oil-suspending agent containing bacillus subtilis R31 living spores can be formed.
Reference examples 10
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) cleaning and sterilizing process: by stand-by granulated sugar tangerine, be immersed in 1000 times of dilute solution process 1min of 40% polyaminopropyl biguanide, remove fresh fruit remained on surface dirt, surperficial pathogenic microorganisms, act on the pathogenic bacteria that partly hide, drain, ventilate;
(3) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The formula (weight ratio) of 40% described polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
Reference examples 11
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the granulated sugar tangerine rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) biological preservation process: by stand-by granulated sugar tangerine, is immersed in 300 times of dilute solution process 2min of 15% bacillus subtilis R31 antistaling agent, is positioned over the vacant room after sterilization, ventilation of windowing after process;
(3) packaging process: choose the rotten fruit of machinery wound after 5 days, and be the Polythene Bag individual packing of 0.03mm with thickness, dress basket is placed in 20-25 DEG C, preserves under relative humidity (RH) 75-90% condition, observe morbidity Decay, often process 180 fruits.The incidence of disease/%=(morbidity fruit number/inspection total fruit number) × 100, commodity rate/%=[(checking the dogberries such as total fruit number-morbidity fruit number-physiological disease)/check total fruit number] × 100
(4) result: in table 1.
The citrus bio-preservative of 15% described bacillus subtilis R31, is every gram and contains 3 × 10
10the microbial bacterial agent product of the bacillus subtilis R31 gemma of cfu.
Composition and the proportioning of the citrus bio-preservative of the present invention 15% bacillus subtilis R31 are as follows:
(1) 15 grams, the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide 30 grams, rapeseed oil 20 grams, Brij 9 grams, Tween-80 (Tween 80) 10 grams, mix and blend 2 hours in mixing kettle;
(2) potassium sorbate 1.5 grams is added in the mixing kettle after stirring 2 hours toward step (1), sodium acetate 2 grams, sucrose 1 gram, and supply rapeseed oil to 100%, continue stirring 0.5 hour, the stable oil-suspending agent containing bacillus subtilis R31 living spores can be formed.
The impact of different embodiments on sugar orange rotting rate and commodity rate sees the following form 1.
Table 1 Preservation Treatment is on the impact of sugar orange rotting rate and commodity rate
Preserve 30 days under room temperature 20-25 DEG C, relative humidity (RH) 75-90% condition, the sugar orange of each processed group is rotten and commodity rate situation is as described in Table 1.As can be known from the results: citrus green ecological preservation method of the present invention can effectively reduce sugar orange rotting rate, improve commodity rate, with the effect quite even better effect of chemical bactericide Prochloraz (reference examples 1); But a kind of citrus green ecological preservation method, disinfecting with biological preservation process is complementary organic whole, the while of only having under service condition, guarantee can be close with chemical bactericide effect, and no matter disinfect or biological preservation process, be used alone the commodity rate that all can not effectively ensure in sugar orange storage period.
Claims (10)
1. a green ecological preservation method for citrus, its step is as follows:
(1) storage fruit is screened: select mechanical injury, without disease and pest, without the citrus fruit rotted, as fresh-retaining preserving fruit, clean stand-by;
(2) disinfect: by citrus fruit stand-by for step (1) gained, be immersed in polyaminopropyl biguanide sterilization listerine process 1-2 minute, to remove fresh citrus fruit remained on surface dirt, surperficial pathogenic microorganisms, to act on the pathogenic bacteria that partly hide, drain for subsequent use;
(3) biological preservation process: the citrus fruit after step (2) being disinfected, is immersed in the biological preservation liquid process 1-2 minute containing bacillus subtilis R31, and process is placed in basket, is placed in sterilization storehouse ventilation airing;
(4) packaging process: choose machinery wound after the citrus fruit after step (3) process is placed 3-5 days and rot really, and use Polythene Bag individual packing, preserve in suitable condition after dress basket.
2. the green ecological preservation method of citrus according to claim 1, it is characterized in that: the sterilization of polyaminopropyl biguanide described in described step (2) listerine, by the polyaminopropyl biguanide aqua product of 40 % by weight, forms by 500 times ~ 1000 multiple dilutions.
3. the green ecological preservation method of citrus according to claim 2, is characterized in that: the formula of the polyaminopropyl biguanide aqua of described 40 % by weight is: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
4. the green ecological preservation method of citrus according to claim 1, it is characterized in that: contain the biological preservation liquid of bacillus subtilis R31 described in described step (3) by the citrus bio-preservative containing bacillus subtilis R31, form by 200 times ~ 800 times multiple dilutions, the described citrus bio-preservative containing bacillus subtilis R31 is wettable powder or oil-suspending agent.
5. a green ecological antistaling agent for citrus, is characterized in that: described antistaling agent is the citrus bio-preservative wettable powder containing bacillus subtilis R31, its component and proportioning as follows:
6. the green ecological antistaling agent of citrus according to claim 5, is characterized in that: the preparation process containing the citrus bio-preservative wettable powder of bacillus subtilis R31 is as follows:
(1) the former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide, wetting agent, dispersant, a certain amount of carrier, co-grinding in pulverizer;
(2) on step (1) basis, add stabilizing agent, and supply carrier, continue to pulverize, must containing the wettable powder of bacillus subtilis R31 living spores.
7. the green ecological antistaling agent of the citrus according to claim 5 or 6, is characterized in that: described wetting agent is AEO, pulls open the mixing of one or more arbitrary proportions in powder, lauryl alcohol polyethylene glycol oxide base ether sodium sulfate, neopelex, calcium dodecyl benzene sulfonate, sldium lauryl sulfate and ethoxylated dodecyl alcohol sodium sulfonate;
Described dispersant is the mixing of one or more arbitrary proportions of dispersing agent MF, lignosulfonates, naphthalenesulfonic acid-formaldehyde condensate, polyvinylpyrrolidone, polyethylene glycol and polyvinyl alcohol;
Described stabilizing agent is the mixing of one or more arbitrary proportions in sucrose, sodium chloride, potassium sorbate and ascorbic acid;
Described carrier is the mixing of one or more arbitrary proportions in precipitated calcium carbonate, kaolin and diatomite.
8. a green ecological antistaling agent for citrus, is characterized in that: described antistaling agent is the citrus bio-preservative oil-suspending agent containing bacillus subtilis R31, its component and proportioning as follows:
9. the green ecological antistaling agent of citrus according to claim 8, is characterized in that: the preparation process of the described citrus bio-preservative oil-suspending agent containing bacillus subtilis R31 is as follows:
(1) the quantitative former powder of bacillus subtilis R31 is taken, chitosan oligosaccharide, carrier, emulsifying agent, mix and blend in mixing kettle;
(2) add stabilizing agent in the mixing kettle after stirring toward step (1), and supply carrier to 100%, continue to stir, form the stable oil-suspending agent containing bacillus subtilis R31 living spores.
10. the green ecological antistaling agent of citrus according to claim 9, is characterized in that: described emulsifying agent is the mixing of one or more arbitrary proportions in polyoxyethylene sorbitol acid anhydride monopalmitate, polyoxyethylene sorbitan monostearate, Tween-80, AEO, Brij and cithrol; Described stabilizing agent is the mixing of one or more arbitrary proportions in potassium sorbate, Sodium Benzoate, sodium chloride, sucrose and sodium acetate; Described carrier is the mixing of one or more arbitrary proportions in soybean oil, rapeseed oil, epoxidized soybean oil and corn oil.
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| CN112336671A (en) * | 2020-11-20 | 2021-02-09 | 山东消博士消毒科技股份有限公司 | Skin-care disinfectant and preparation method thereof |
| CN115399315A (en) * | 2022-08-30 | 2022-11-29 | 凡美(广州)农业科技有限公司 | Fresh flower preservative and preparation method thereof |
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