CN104911230B - The method that in-situ fermentation prepares bacteria cellulose - Google Patents
The method that in-situ fermentation prepares bacteria cellulose Download PDFInfo
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- CN104911230B CN104911230B CN201510391158.0A CN201510391158A CN104911230B CN 104911230 B CN104911230 B CN 104911230B CN 201510391158 A CN201510391158 A CN 201510391158A CN 104911230 B CN104911230 B CN 104911230B
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Abstract
The invention discloses a kind of method that in-situ fermentation prepares bacteria cellulose, by adding nonmetallic ore inorganic gel into fermentation medium for bacterial cellulose, then adds production bacterial cellulose strain, and after certain time ferments, separation obtains bacterial cellulose product.The present invention prepares bacteria cellulose using nonmetallic ore inorganic gel, realize the high-valued application of nonmetallic ore, the addition of inorganic gel improves the water absorbing properties or water holding capacity of bacteria cellulose, in-situ fermentation pattern makes fermentation be completed with bacteria cellulose modification in same system, greatly shortens technological process and time.
Description
Technical field
The present invention relates to the preparation method of bacteria cellulose, and in particular to one kind addition nonmetallic ore inorganic gel carries out former
The method that position fermentation prepares bacteria cellulose, belongs to biological chemical field.
Background technology
Bacteria cellulose（Bacterial cellulose）It is a kind of boiomacromolecule chemical combination synthesized by some bacteriums
Thing.Different from plant cellulose, bacteria cellulose is not the constituent of cell membrane, but bacterial secretory is to extracellular product,
It is in independent filamentary fibers form, and the plant cellulose impurity such as the lignin that undopes, hemicellulose.With plant cellulose phase
Than bacteria cellulose has many unique physicochemical properties and mechanical performance, includes ultra-fine property（Nanoscale）, high-purity, height
Crystallinity；Exist in the form of Single Fiber, there is consistent molecularly oriented；High Young's modulus, tensile strength and shape maintain energy
Power；Extremely strong moisture holding capacity；Higher biocompatibility；Shape and performance are controllable etc..Due to its unique physicochemical property and
Mechanical performance, bacteria cellulose are widely used in the fields such as environmental protection, papermaking, food, macromolecule, medical treatment.
In order to further improve the water absorbing properties of bacteria cellulose, it is necessary to which appendix is expected to increase it in bacteria cellulose
The group of water absorbing properties.Bacteria cellulose in-situ fermentation (in situFermentation) refer to and different changes is added before fermentation
Compound, then carry out bacteria cellulose fermentation, fermentation while, compound can appendix product is realized on bacteria cellulose
It is in-situ modified.In order to realize bacteria cellulose water suction and the raising of water retention property, added in the medium using in-situ fermentation technology
Add xenobiotic substrates.In numerous substrates, inorganic gel turns into the preferable xenobiotic substrates of super water-absorbent energy bacteria cellulose fermentation,
Its advantage has two aspects, first, inorganic gel, which has, meets water-swellable physical property, it is expected the suction for strengthening bacteria cellulose
Water-based energy；Second, inorganic gel itself is nontoxic, institute is compatible with general aqueous ferment environment, does not influence bacteria cellulose and fermented
Journey.
The content of the invention
The purpose of the present invention is：A kind of method that in-situ fermentation prepares bacteria cellulose is provided, by adding nonmetallic ore
Inorganic gel carries out in-situ fermentation and prepares bacteria cellulose, and nonmetallic ore raw material sources are extensive, cheap, and nothing is prepared using it
Machine gel industrially has broad prospects.
The present invention is achieved by the following technical programs：Nonmetallic ore inorganic gel is added first to bacterial fibers
In plain fermentation medium, then production bacterial cellulose strain is added, after certain time ferments, separation obtains bacteria cellulose production
Product.
Wherein, the nonmetallic ore inorganic gel includes bentonite inorganic gel, attapulgite inorganic gel；In culture medium
Inorganic gel addition is 0.5-5 g/L.
Wherein, fermentation medium for bacterial cellulose is HS culture mediums or ligno-cellulose hydrolysate；Produce bacteria cellulose bacterium
Strain is acetobacter xylinum.
Wherein, fermentation method includes dynamic fermentation and static fermentation, fermentation temperature 20-35oC；Inoculum concentration is 1-15%
(v/v)。
Wherein, bacteria cellulose separation method is centrifugation or filtering.
The beneficial effects of the invention are as follows：Bacteria cellulose is prepared using nonmetallic ore inorganic gel, realizes nonmetallic ore
High-valued application, the addition of inorganic gel improve the water absorbing properties or water holding capacity of bacteria cellulose, and in-situ fermentation pattern makes hair
Ferment is completed with bacteria cellulose modification in same system, greatly shortens technological process and time.
Embodiment
Technical scheme is described further below in conjunction with specific embodiment, embodiment is not to the present invention
Limitation.
Embodiment 1：0.5 g/L bentonite inorganic gels add and in-situ fermentation system are formed in HS culture mediums, add in system
Enter static fermentation 20 days, fermentation temperature 20 after acetobacter xylinumoC, inoculum concentration are 1% (v/v)；After fermentation ends, filtering obtains
Bacterial cellulose product, products obtained therefrom water-holding capacity ratio are not added with inorganic gel group and improve 47.0%.
Embodiment 2：5 g/L attapulgites inorganic gels, which add, forms in-situ fermentation body in Corncob hydrolysate culture medium
System, dynamic fermentation one week, fermentation temperature 25 after acetobacter xylinum are added in systemoC, inoculum concentration are 15% (v/v)；Fermentation ends
Afterwards, centrifugation obtains bacterial cellulose product, and products obtained therefrom water holding capacity ratio is not added with inorganic gel group and improves 20.0%.
Embodiment 3：2.5 g/L attapulgites inorganic gels add and in-situ fermentation system are formed in HS culture mediums, in system
Dynamic fermentation 4 days, fermentation temperature 30 after addition acetobacter xylinumoC, inoculum concentration are 8% (v/v)；After fermentation ends, centrifugation obtains
Bacterial cellulose product, products obtained therefrom water-holding capacity ratio are not added with inorganic gel group and improve 25.0%.
Embodiment 4：1 g/L bentonite inorganic gels add in bagasse hydrolysis liquid culture medium and form in-situ fermentation system,
Static fermentation two weeks after acetobacter xylinum, fermentation temperature 35 are added in systemoC, inoculum concentration are 10% (v/v)；After fermentation ends,
Filtering obtains bacterial cellulose product, and products obtained therefrom water holding capacity ratio is not added with inorganic gel group and improves 15.0%.
Claims (1)
1. the method that in-situ fermentation prepares bacteria cellulose, nonmetallic ore inorganic gel to bacteria cellulose fermentation is added first and is trained
Support in base, then add production bacterial cellulose strain, after certain time ferments, separation obtains bacterial cellulose product；It is special
Sign is：The nonmetallic ore inorganic gel includes bentonite inorganic gel, attapulgite inorganic gel；Inorganic gel in culture medium
Addition is 0.5-5 g/L；Fermentation medium for bacterial cellulose is HS culture mediums or ligno-cellulose hydrolysate；Produce bacterial fibers
Plain bacterial strain is acetobacter xylinum；Fermentation method includes dynamic fermentation and static fermentation；Fermentation temperature is 20-35 DEG C, inoculum concentration 1-
15% (v/v), fermentation time are 4-20 days；Bacteria cellulose separation method is centrifugation or filtering.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107698805A (en) * | 2016-08-09 | 2018-02-16 | 丁玉琴 | A kind of defervescence plaster used preparation method with compound water congealing glued membrane |
| CN106831123A (en) * | 2017-01-19 | 2017-06-13 | 南京理工大学 | A kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer and preparation method thereof |
| CN107044054A (en) * | 2017-04-12 | 2017-08-15 | 江南大学 | A kind of bacteria cellulose in situ/eider down insulation material and preparation method thereof |
| CN110563886A (en) * | 2018-06-06 | 2019-12-13 | 中国科学院广州能源研究所 | bacterial cellulose-based super absorbent material and preparation method thereof |
| CN109248663A (en) * | 2018-09-28 | 2019-01-22 | 芜湖市鹏磊新材料有限公司 | A kind of preparation method of use in waste water treatment bentonite adsorption material |
| CN112695009B (en) * | 2021-01-29 | 2023-01-06 | 广东药科大学 | Method for preparing bacterial cellulose by in-situ fermentation |
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Address after: 211700 Dongfang Road, Xuyi County, Huaian, Jiangsu Province, No. 3 Patentee after: Xuyi Aotu energy and environmental protection materials R & D Center Patentee after: GUANGZHOU INSTITUTE OF ENERGY CONVERSION, CHINESE ACADEMY OF SCIENCES Address before: 211700 Dongfang Road, Xuyi County, Huaian, Jiangsu Province, No. 3 Patentee before: XUYI ATTAPULGITE RESEARCH & DEVELOPMENT CENTER OF GUANGZHOU INSTITUTE OF ENERGY CONVERSION, CHINESE ACADEMY OF SCIENCES Patentee before: GUANGZHOU INSTITUTE OF ENERGY CONVERSION, CHINESE ACADEMY OF SCIENCES |