CN104860749A - Flammulina velutipes culture medium - Google Patents
Flammulina velutipes culture medium Download PDFInfo
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- CN104860749A CN104860749A CN201510191386.3A CN201510191386A CN104860749A CN 104860749 A CN104860749 A CN 104860749A CN 201510191386 A CN201510191386 A CN 201510191386A CN 104860749 A CN104860749 A CN 104860749A
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Abstract
The invention discloses a flammulina velutipes culture medium, and relates to the technical field of mushroom planting. The culture medium is prepared from the raw materials of, by weight, 100-150 parts of corn stalk, 30-60 parts of soybean, 40-60 parts of brewer's grain, 35-40 parts of buckwheat kernel, 15-20 parts of shell powder, 10-20 parts of lotus leaf powder, 10-20 parts of longan shell, 8-10 parts of gypsum powder, 10-20 parts of ammonium sulfate, 8-10 parts of slaked lime, 8-10 parts of arrowroot powder, 3-5 parts of ant powder, 10-20 parts of glucose, 2-4 parts of peptone, 1-3 parts of bitter orange, 1-3 parts of sodium lactate, 0.4-0.8 parts of polyglutamic acid, and 0.6-1 parts of lactobacillus. With the culture medium provided by the invention, flammulina velutipes quality and yield are both improved. Mycelia growth speed is high, full-bag period is short, inoculation success rate is high, and harvest rate is high.
Description
Technical field
The present invention relates to mushroom planting technology field, especially relate to a kind of method of culture medium for golden mushroom containing herbal medicine and use substratum cultivating flammulina velutipes.
Background technology
Needle mushroom formal name used at school hair handle money bacterium, more complete containing essential amino acid composition, wherein Methionin and arginine content are especially abundant, and zinc content is higher, especially good effect is had to the height of children and intelligent growth to increasing intelligence, person " increases intelligence mushroom ", also containing a kind of material being Flammulin in needle mushroom, there is enhancing body to the resist ability of cancer cells, often edible needle mushroom, not only can prevention and therapy hepatopathy and stomach, enteron aisle ulcer, and applicable hyperpietic, obese person and the elderly eat, energy decreasing cholesterol, prevention of liver disease and intestines and stomach ulcer, enhancing body healthy tendency, illness prevention and fitness.
Needle mushroom is because of its delicious flavour, nutritious and extensively like by human consumer, and it can only absorb nutritive substance from planting material, and its required nutrition of growing comprises carbohydrate and nitrogenous compound, and a small amount of inorganic salt and VITAMIN etc.Therefore, planting material has a great impact yield of flammulina velutipes and quality.At present, the sufficient wheat straw that the substratum of known plantation needle mushroom generally uses, cotton seed hulls are main raw material, and so not only at the bottom of output, main deficiency is that the nutritive ingredient of needle mushroom is single.
Summary of the invention
Goal of the invention of the present invention is: for above-mentioned Problems existing, provides a kind of culture medium for golden mushroom, and the needle mushroom quality using substratum of the present invention to plant and output all increase; Its mycelial growth rate is high, and the purseful cycle is short, success ratio of inoculation and yield rate high.
The technical solution used in the present invention is as follows:
A kind of culture medium for golden mushroom is be made up of the raw material of following weight part: maize straw 100-150 part, soya-bean cake 30-60 part, brewer's grains 40-60 part, shelled buckwheat 35-40 part, oyster shell whiting 15-20 part, lotus leaf powder 10-20 part, Pericarpium Longan 10-20 part, terra alba 8-10 part, ammonium sulfate 10-20 part, white lime 8-10 part, powder of Radix Puerariae 8-10 part, Formica fusca powder 3-5 part, glucose 10-20 part, peptone 2-4 part, Fructus Aurantii 1-3 part, Sodium.alpha.-hydroxypropionate 1-3 part, polyglutamic acid 0.4-0.8 part, milk-acid bacteria 0.6-1 part.
Further preferably, described substratum is made up of the raw material of following weight part: maize straw 120 parts, soya-bean cake 50 parts, brewer's grains 50 parts, shelled buckwheat 38 parts, oyster shell whiting 18 parts, lotus leaf powder 15 parts, Pericarpium Longan 15 parts, terra alba 9 parts, 12 parts, ammonium sulfate, white lime 9 parts, powder of Radix Puerariae 9 parts, Formica fusca powder 4 parts, glucose 15 parts, peptone 3 parts, Fructus Aurantii 2 parts, Sodium.alpha.-hydroxypropionate 2 parts, polyglutamic acid 0.6 part, milk-acid bacteria 0.8 part.
The present invention also provides a kind of culture medium for golden mushroom to carry out the method for golden mushroom plantation, comprises the following steps:
(1) prepare burden: get described component respectively and carry out the rear poach of fragmentation, the water content of substratum is made to be 60%-65%, the substratum prepared is piled stockpile ferment, after 3-4 days, observe after material temperature reaches 55-60 DEG C and carry out turning, continue fermentation after turning, treat that material temperature reaches 55-60 DEG C of secondary fermentation again and terminates;
(2) disinfection inoculation: after the substratum pack after fermentation, carry out High Temperature Sterilization at 110-120 DEG C, obtained cultivating bag; Described cultivating bag material temperature degree is down to less than 27 DEG C, carries out strain inoculation in an aseptic environment;
(3) cultivate: postvaccinal cultivating bag is placed in culturing room, is adjusted to half-light in culturing room, temperature controls at 20 DEG C-24 DEG C, indoor carbon dioxide concentration controls at 2000-3500mg/kg, cloudy light cultivates 20-29 days, after mycelia covers with culture bag, cultivating bag is moved into mushroom room and carries out mycelium stimulation;
(4) after sporophore grows, culturing room's temperature controls between 12 ~ 17 DEG C, and relative air humidity keeps 90% ~ 95%, and carries out ventilation; Culturing room's half-light controls;
(5) stem grows to 13 ~ 14 centimetres, carries out finished product and gather during bacteria cover diameter 2 ~ 3 centimetres.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows: a kind of culture medium for golden mushroom of the present invention and golden mushroom plantation method, the collocation of substratum batching Middle nutrition element is scientific and reasonable, meet the growth demand of needle mushroom, through the needle mushroom repetition test of the present inventor by output, find that needle mushroom taste is fresh and tender, delicious good to eat; By cultivating method of the present invention, the quality of needle mushroom and output are all increased; Its mycelial growth rate is high, and the purseful cycle is short, success ratio of inoculation and yield rate high.
Embodiment
The invention discloses a kind of culture medium for golden mushroom, and utilize the method for substratum cultivating flammulina velutipes of the present invention.Below by way of specific embodiment, the invention will be further described.
Embodiment 1
A kind of culture medium for golden mushroom is be made up of following raw material: maize straw 100kg, soya-bean cake 30kg, brewer's grains 40kg, shelled buckwheat 35kg, oyster shell whiting 15kg, lotus leaf powder 10kg, Pericarpium Longan 10kg, terra alba 8kg, ammonium sulfate 10kg, white lime 8kg, powder of Radix Puerariae 8kg, Formica fusca powder 3kg, glucose 10kg, peptone 2kg, Fructus Aurantii 1kg, Sodium.alpha.-hydroxypropionate 1kg, polyglutamic acid 0.4kg, milk-acid bacteria 0.6kg.
Utilize above-mentioned culture medium for golden mushroom to carry out the method for golden mushroom plantation, comprise the following steps:
(1) prepare burden: get described component respectively and carry out the rear poach of fragmentation, make the water content of substratum be 60%, the substratum prepared is piled stockpile and ferments, after 3 days, observe after material temperature reaches 55 DEG C and carry out turning, continue fermentation after turning, treat that material temperature reaches 55 DEG C of secondary fermentation again and terminates;
(2) disinfection inoculation: after the substratum pack after fermentation, carry out High Temperature Sterilization at 110 DEG C, obtained cultivating bag; Described cultivating bag material temperature degree is down to less than 27 DEG C, carries out strain inoculation in an aseptic environment;
(3) cultivate: postvaccinal cultivating bag is placed in culturing room, half-light is adjusted in culturing room, temperature controls at 20 DEG C, indoor carbon dioxide concentration controls at 2000mg/kg, cloudy light cultivates 29 days, after mycelia covers with culture bag, cultivating bag is moved into mushroom room and carries out mycelium stimulation, the old bacterial classification being about to surface is wiped off, to promote former base and fruit-body formation;
(4) after sporophore grows, culturing room's temperature controls at about 12 DEG C, and relative air humidity keeps about 90%, and carries out ventilation; Culturing room's half-light controls;
(5) stem grows to 13 ~ 14 centimetres, carries out finished product and gather during bacteria cover diameter 2 ~ 3 centimetres, obtains finished product needle mushroom 560kg.
Embodiment 2
A kind of culture medium for golden mushroom is be made up of following raw material: maize straw 120kg, soya-bean cake 50kg, brewer's grains 50kg, shelled buckwheat 38kg, oyster shell whiting 18kg, lotus leaf powder 15kg, Pericarpium Longan 15kg, terra alba 9kg, ammonium sulfate 12kg, white lime 9kg, powder of Radix Puerariae 9kg, Formica fusca powder 4kg, glucose 15kg, peptone 3kg, Fructus Aurantii 2kg, Sodium.alpha.-hydroxypropionate 2kg, polyglutamic acid 0.6kg, milk-acid bacteria 0.8kg.
Utilize above-mentioned culture medium for golden mushroom to carry out the method for golden mushroom plantation, comprise the following steps:
(1) prepare burden: get described component respectively and carry out the rear poach of fragmentation, the water content of substratum is made to be about 65%, the substratum prepared is piled stockpile ferment, after 4 days, turning is carried out after observing material temperature 55-60 DEG C, continue fermentation after turning, treat that material temperature reaches 60 DEG C of secondary fermentation again and terminates;
(2) disinfection inoculation: after the substratum pack after fermentation, carry out High Temperature Sterilization at 120 DEG C, obtained cultivating bag; Described cultivating bag material temperature degree is down to less than 27 DEG C, carries out strain inoculation in an aseptic environment;
(3) cultivate: postvaccinal cultivating bag is placed in culturing room, half-light is adjusted in culturing room, temperature controls at about 24 DEG C, indoor carbon dioxide concentration controls at 3500mg/kg, cloudy light cultivates about 25 days, after mycelia covers with culture bag, cultivating bag is moved into mushroom room and carries out mycelium stimulation, the old bacterial classification being about to surface is wiped off, to promote former base and fruit-body formation;
(4) after sporophore grows, culturing room's temperature controls at about 17 DEG C, and relative air humidity remains on about 95%, and carries out ventilation; Culturing room's half-light controls;
(5) stem grows to 13 ~ 14 centimetres, carries out finished product and gather during bacteria cover diameter 2 ~ 3 centimetres, obtains finished product needle mushroom 580kg.
Embodiment 3
A kind of culture medium for golden mushroom is be made up of following raw material: maize straw 150kg, soya-bean cake 60kg, brewer's grains 60kg, shelled buckwheat 40kg, oyster shell whiting 20kg, lotus leaf powder 20kg, Pericarpium Longan 20kg, terra alba 10kg, ammonium sulfate 20kg, white lime 10kg, powder of Radix Puerariae 10kg, Formica fusca powder 5kg, glucose 20kg, peptone 4kg, Fructus Aurantii 3kg, Sodium.alpha.-hydroxypropionate 3kg, polyglutamic acid 0.8kg, milk-acid bacteria 1kg.
Utilize above-mentioned culture medium for golden mushroom to carry out the method for golden mushroom plantation, comprise the following steps:
(1) prepare burden: get described component respectively and carry out the rear poach of fragmentation, the water content of substratum is made to be about 65%, the substratum prepared is piled stockpile ferment, after 4 days, observe after material temperature reaches 58 DEG C and carry out turning, continue fermentation after turning, treat that material temperature reaches 58 DEG C of secondary fermentation again and terminates;
(2) disinfection inoculation: after the substratum pack after fermentation, carry out High Temperature Sterilization at 110 DEG C, obtained cultivating bag; Described cultivating bag material temperature degree is down to less than 27 DEG C, carries out strain inoculation in an aseptic environment;
(3) cultivate: postvaccinal cultivating bag is placed in culturing room, half-light is adjusted in culturing room, temperature controls at 22 DEG C, indoor carbon dioxide concentration controls at 3000mg/kg, cloudy light cultivates 20 days, after mycelia covers with culture bag, cultivating bag is moved into mushroom room and carries out mycelium stimulation, the old bacterial classification being about to surface is wiped off, to promote former base and fruit-body formation;
(4) after sporophore grows, culturing room's temperature controls at about 15 DEG C, and relative air humidity keeps about 92%, and carries out ventilation; Culturing room's half-light controls;
(5) stem grows to 13 ~ 14 centimetres, carries out finished product and gather during bacteria cover diameter 2 ~ 3 centimetres, obtains finished product needle mushroom 600kg.
Comparative example
With corn cob 300kg, wheat bran 125kg, gypsum 10kg makes culture medium raw material, according to the method cultivating flammulina velutipes that embodiment 3 is identical, obtains finished product needle mushroom 520kg.
In the embodiment of the present invention and comparative example, Growth of Flammulina Velutipes situation is as shown in table 1.
Table 1 golden mushroom plantation and prior art cultivate information slip
Sequence number | Mycelium length (cm/ day) | The purseful cycle (day) | Success ratio of inoculation (%) | Yield rate (%) |
Embodiment 1 | 0.36 | 4 | 98 | 130 |
Embodiment 2 | 0.38 | 4 | 99 | 129 |
Embodiment 3 | 0.40 | 5 | 99 | 132 |
Comparative example | 0.33 | 6 | 98 | 125 |
In upper table 1, after mycelium length fingering enters culture cycle, the day growth speed of mycelia, the purseful cycle, yield rate referred to the weight ratio between the finished product needle mushroom of results and substratum namely from being seeded to the cycle that mycelia is full of cultivating bag.From form 1, use the present invention to carry out golden mushroom plantation, its mycelial growth rate is higher than comparative example, and success ratio of inoculation and yield rate, higher than comparative example, had good economic benefit, were worthy of popularization lower than comparative example the purseful cycle.
Claims (3)
1. a culture medium for golden mushroom, is characterized in that: described substratum is made up of the raw material of following weight part: maize straw 100-150 part, soya-bean cake 30-60 part, brewer's grains 40-60 part, shelled buckwheat 35-40 part, oyster shell whiting 15-20 part, lotus leaf powder 10-20 part, Pericarpium Longan 10-20 part, terra alba 8-10 part, ammonium sulfate 10-20 part, white lime 8-10 part, powder of Radix Puerariae 8-10 part, Formica fusca powder 3-5 part, glucose 10-20 part, peptone 2-4 part, Fructus Aurantii 1-3 part, Sodium.alpha.-hydroxypropionate 1-3 part, polyglutamic acid 0.4-0.8 part, milk-acid bacteria 0.6-1 part.
2. a kind of culture medium for golden mushroom according to claim 1, it is characterized in that: described substratum is made up of the raw material of following weight part: maize straw 120 parts, soya-bean cake 50 parts, brewer's grains 50 parts, shelled buckwheat 38 parts, oyster shell whiting 18 parts, lotus leaf powder 15 parts, Pericarpium Longan 15 parts, terra alba 9 parts, 12 parts, ammonium sulfate, white lime 9 parts, powder of Radix Puerariae 9 parts, Formica fusca powder 4 parts, glucose 15 parts, peptone 3 parts, Fructus Aurantii 2 parts, Sodium.alpha.-hydroxypropionate 2 parts, polyglutamic acid 0.6 part, milk-acid bacteria 0.8 part.
3. utilize a kind of culture medium for golden mushroom described in claim 1 or 2 to carry out the method for golden mushroom plantation, comprise the following steps:
(1) prepare burden: get described component respectively and carry out the rear poach of fragmentation, the water content of substratum is made to be 60%-65%, the substratum prepared is piled stockpile ferment, after 3-4 days, observe after material temperature reaches 55-60 DEG C and carry out turning, continue fermentation after turning, treat that material temperature reaches 55-60 DEG C of secondary fermentation again and terminates;
(2) disinfection inoculation: after the substratum pack after fermentation, carry out High Temperature Sterilization at 110-120 DEG C, obtained cultivating bag; Described cultivating bag material temperature degree is down to less than 27 DEG C, carries out strain inoculation in an aseptic environment;
(3) cultivate: postvaccinal cultivating bag is placed in culturing room, is adjusted to half-light in culturing room, temperature controls at 20 DEG C-24 DEG C, indoor carbon dioxide concentration controls at 2000-3500mg/kg, cloudy light cultivates 20-29 days, after mycelia covers with culture bag, cultivating bag is moved into mushroom room and carries out mycelium stimulation;
(4) after sporophore grows, culturing room's temperature controls between 12 ~ 17 DEG C, and relative air humidity keeps 90% ~ 95%, and carries out ventilation; Culturing room's half-light controls;
(5) stem grows to 13 ~ 14 centimetres, carries out finished product and gather during bacteria cover diameter 2 ~ 3 centimetres.
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Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105112300A (en) * | 2015-08-31 | 2015-12-02 | 雷色香 | Needle mushroom mycelium blocks |
CN105237118A (en) * | 2015-08-27 | 2016-01-13 | 马鞍山市安康菌业有限公司 | High-effective culture medium being helpful to promote growth and metabolism of flammulina velutipes and preparation method thereof |
CN105503434A (en) * | 2016-01-06 | 2016-04-20 | 安庆市大源山农业科技有限公司 | Flammulina velutipes culture medium |
CN105493898A (en) * | 2016-01-06 | 2016-04-20 | 安庆市大源山农业科技有限公司 | Culture method of needle mushrooms |
CN106542860A (en) * | 2016-10-31 | 2017-03-29 | 嘉善弘欣化工厂(普通合伙) | Zinc-rich Edible Fungi method and culture medium prescription |
CN107135805A (en) * | 2017-05-02 | 2017-09-08 | 安徽铜仙子食用菌科技有限公司 | A kind of asparagus ecology planting method based on high nutrition |
CN107434722A (en) * | 2016-05-27 | 2017-12-05 | 临桂铭泰果蔬农民专业合作社 | The rainbow conk compost made of pueraria root residue grape branch |
CN107434728A (en) * | 2016-05-27 | 2017-12-05 | 临桂铭泰果蔬农民专业合作社 | The mushroom cultivation material made of pueraria root residue grape branch |
CN107926485A (en) * | 2017-12-05 | 2018-04-20 | 罗成喜 | A kind of culture medium of needle mushroom and preparation method thereof |
CN107954784A (en) * | 2017-12-13 | 2018-04-24 | 佛山市高明区生产力促进中心 | A kind of needle mushroom implantation methods with higher economic well-being of workers and staff |
CN108178678A (en) * | 2018-03-15 | 2018-06-19 | 夏文秀 | A kind of culture medium for golden mushroom material and preparation method thereof |
CN109348982A (en) * | 2016-09-14 | 2019-02-19 | 上海昌高科技发展有限公司 | A kind of cultural method and its culture medium of edible mushroom |
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Cited By (12)
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CN105237118A (en) * | 2015-08-27 | 2016-01-13 | 马鞍山市安康菌业有限公司 | High-effective culture medium being helpful to promote growth and metabolism of flammulina velutipes and preparation method thereof |
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CN105503434A (en) * | 2016-01-06 | 2016-04-20 | 安庆市大源山农业科技有限公司 | Flammulina velutipes culture medium |
CN105493898A (en) * | 2016-01-06 | 2016-04-20 | 安庆市大源山农业科技有限公司 | Culture method of needle mushrooms |
CN107434722A (en) * | 2016-05-27 | 2017-12-05 | 临桂铭泰果蔬农民专业合作社 | The rainbow conk compost made of pueraria root residue grape branch |
CN107434728A (en) * | 2016-05-27 | 2017-12-05 | 临桂铭泰果蔬农民专业合作社 | The mushroom cultivation material made of pueraria root residue grape branch |
CN109348982A (en) * | 2016-09-14 | 2019-02-19 | 上海昌高科技发展有限公司 | A kind of cultural method and its culture medium of edible mushroom |
CN106542860A (en) * | 2016-10-31 | 2017-03-29 | 嘉善弘欣化工厂(普通合伙) | Zinc-rich Edible Fungi method and culture medium prescription |
CN107135805A (en) * | 2017-05-02 | 2017-09-08 | 安徽铜仙子食用菌科技有限公司 | A kind of asparagus ecology planting method based on high nutrition |
CN107926485A (en) * | 2017-12-05 | 2018-04-20 | 罗成喜 | A kind of culture medium of needle mushroom and preparation method thereof |
CN107954784A (en) * | 2017-12-13 | 2018-04-24 | 佛山市高明区生产力促进中心 | A kind of needle mushroom implantation methods with higher economic well-being of workers and staff |
CN108178678A (en) * | 2018-03-15 | 2018-06-19 | 夏文秀 | A kind of culture medium for golden mushroom material and preparation method thereof |
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