CN104856067A - Cistanche or cynomorium songaricum ferment beef jerky and preparation method therefor - Google Patents
Cistanche or cynomorium songaricum ferment beef jerky and preparation method therefor Download PDFInfo
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- CN104856067A CN104856067A CN201510220371.5A CN201510220371A CN104856067A CN 104856067 A CN104856067 A CN 104856067A CN 201510220371 A CN201510220371 A CN 201510220371A CN 104856067 A CN104856067 A CN 104856067A
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- cynomorium songaricum
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- fermentation
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to the field of food processing, particularly to a cistanche or cynomorium songaricum ferment beef jerky and a manufacturing method therefor. The cistanche or cynomorium songaricum ferment contained in the beef jerky provided by the invention is obtained by virtue of a modern biological fermentation technology by taking cistanche or cynomorium songaricum as a core matrix. The fermentation modes for the invention comprise a liquid fermentation method and a solid fermentation method. Compared with the prior art, the cistanche or cynomorium songaricum ferment beef jerky provided by the invention has the advantages and effects that: in the preparation process, the nutritional components of the raw material cistanche or cynomorium songaricum is not wasted or damaged, and the nutritional and health-care values of the prepared cistanche or cynomorium songaricum ferment are remarkably improved; the cistanche or cynomorium songaricum ferment beef jerky is simple in preparation process and controllable in preparation procedure; and the obtained ferment product is stable in quality, can be stored at normal temperature, is suitable for scaled production and has a good market prospect. The cistanche or cynomorium songaricum ferment beef jerky provided by the invention is suitable for all people to eat and has the functions of enhancing the immunity and the memory, or tonifying the kidney and securing essence in daily life.
Description
Technical field
The present invention relates to food processing field, be specifically related to a kind of saline cistanche or cynomorium songaricum ferment dried beef and preparation method thereof.
Background technology
Ferment is also called " enzyme ", and be the material of biological Nature creating own, ferment is the large biological molecule with biocatalytic Activity, i.e. biocatalyst.It can accelerate the speed of biochemical reaction, but does not change direction and the product of reaction.That is, ferment can only be used for the speed accelerating all kinds of biochemical reaction, but is not biochemical reaction itself.It is present in the animal and plant body of all work, is the required material of one maintaining the vital movements such as body normal function, digest food, repair tissue.
Ferment has emerged at the study and utilization in the fields such as nutrition and health care, clinical medicine, aesthetic nursing, weight-reducing, health care, Subhealth treating, the stock substrate of current production ferment is mainly based on cereal, veterinary antibiotics, edible mushroom, and the research for Chinese medicine ferment is less.
But fermentation method is one of method of Chinese medicine preparation always, it by microorganism effect, change original property of medicine, improve curative effect, reduce toxic and side effect, expand indication.The various enzymes that microorganism produces in growth course kind, are converted into new active component or are decomposed by toxic component and lower the toxic and side effect of medicine by the ingredient breakdown of medicine.Combine with traditional method of Chinese medicinal with modern biotechnology, biofermentation technique is applied in Chinese medicine development, make the effectively large molecule of medicine Absorbable rod become Small molecular and be more easily absorbed by the body, more easily arrive target organ and play a role.Moreover, in the Chinese medicine preparation that applying biological zymotechnique is produced, physiological activator not only containing Chinese medicine itself, but also be rich in the trace element of multiple thing of supporting one's family, abundant amino acid and the multiple beneficial that bacterial classification and matrix produce in fermentation preparation process, the combination of these nutritional labelings rationally, very easily absorbed by body and utilize, reaching the object of integration of drinking and medicinal herbs.
Saline cistanche is loaded in Shennong's Herbal, is classified as top grade, the effect such as have kidney-replenishing, benefiting essence-blood, relax bowel.The saline cistanche that " Chinese Pharmacopoeia " records is the fleshy stem of orobanchaceae plant cistanche and Cistanche tubulosa dry zone scale leaf.Saline cistanche is mainly containing the chemical composition such as benzyl carbinol glycosides, iridoid glycoside, lignanoid and sterol, and wherein phenylethanoid glycosides is the main active in saline cistanche, have establishing-Yang, anti-oxidant, anti-ageing, improve immunity, strengthen the several functions such as memory.
Cynomorium songaricum is the medicine simply of kidney tonifying, it can flat liver kidney tonifying, beneficial intensive culture blood, relax bowel, the infertility that causes for the treatment of insufficiency of vital energy and blood, also can strengthening the muscles and bones, supplement calcium.Very large benefit is had to function of human body; Strengthen immunologic function; Scavenging free radicals; Platelet aggregation-against; There is the effect of carbohydrate cortin sample; Replenishing vitamins and mineral matter.Up-to-date scientific experiment proves that cynomorium songaricum also has anti-cancer, antiviral, the effect that delays senility.Be applicable to hypoimmunity, easy infection disease patient; Young and middle-aged hardworking cause and healthy overdraw person; The multiple patients with chronic diseases such as frequent micturition constipation, insomnia alopecia, asthma, the weak premature ejaculation of impotence.
Take beef as the dried beef that Raw material processing becomes, the several mineral materials containing needed by human body and amino acid, both maintained the resistance to feature of chewing of beef, stored for a long timely again never degenerates.Develop so far from first cavalryman's invention dried beef, dried beef technique is constantly innovated improvement by descendant, till now various in style, taste is different.Along with China's expanding economy, people are more and more various for the quality of dried beef, safety, local flavor, mouthfeel demand.But.Traditional dried beef color and taste is single, rare change, and food of a specified duration makes us losing interest, so that the requirement of people to its taste and health-care effect also becomes clear day by day.
By saline cistanche or Cynomorium fermented preparation saline cistanche or cynomorium songaricum ferment and then the dried beef of preparation containing saline cistanche or cynomorium songaricum ferment are not also studied in the prior art.
Summary of the invention
The invention provides a kind of dried beef containing saline cistanche or cynomorium songaricum ferment and preparation method thereof, wherein contained saline cistanche or cynomorium songaricum ferment do not have the nutritional labeling of losing, destroying saline cistanche or cynomorium songaricum raw material in preparation process, and the nutrition and health care that obtained saline cistanche or cynomorium songaricum ferment significantly improve dried beef are worth.
The dried beef that the present invention contains saline cistanche or cynomorium songaricum ferment is after the dried beef adopting conventional method to obtain is placed in saline cistanche or cynomorium songaricum ferment infuse, air-dry or dry and obtain, the content of described saline cistanche or cynomorium songaricum ferment is 0.5-8%, preferred 3-5% (g/100mL); Or the dried beef adopting conventional method to obtain is mixed with saline cistanche or cynomorium songaricum ferment powder or mixes with the saline cistanche or cynomorium songaricum ferment powder that contain flavoring, make the outer uniform adhesion ferment powder of dried beef and obtain.Further, the time of described infuse is 1-5 hour.Described flavoring is one or more in sugar, salt, soy sauce, bent wine, monosodium glutamate, ginger, garlic, five-spice powder or fennel powder.Or in the process of beef boiling, add 0.5-8%, the saline cistanche of preferred 3-5% (g/100mL) or cynomorium songaricum ferment.
Above-mentioned conventional method can comprise the following steps:
(1) preparation of beef gruel: boned by beef, clean, drain, puts into cutmixer by raw meat and cuts into meat gruel, and adds flavoring mixing, mixes 6-12h under 0-4 DEG C of condition;
(2) die-filling boiling: the beef gruel of mixing loaded in grinding tool, builds and boils 2-4h in the pot being placed in 80-100 DEG C;
(3) cooling, section, vegetable oil smear;
(4) baking: toast 2-4h at sliced meat being placed in 80 DEG C-100 DEG C.
Above-mentioned conventional method also can comprise the following steps:
(1) select the forward and backward das Beinfleisch of ox, reject fat and muscle tendon etc., clean, drain, be cut into the cube meat of 0.2-0.3kg;
(2) precook: cube meat is put into pot, clear water is boiled, and boils 20-30min, picks up cooling, and former soup is stand-by;
(3) stripping and slicing: to cut into inch strips by different size, block or fourth, in the same size, thickness is even;
(4) halogen boils: the cutlet cut, cube meat or meat cubelets are put into former soup, adds flavoring, first simmers with moderate heat with after big fire boiling, finally receives juice with slow fire, treats that thick gravy is received dry, can be off the pot;
(5) dewater: in ventilation natural air drying or oven dry.
For saline cistanche of the present invention or cynomorium songaricum ferment be with saline cistanche or cynomorium songaricum for core matrix, obtained by modern biotechnology fermentation technique.
For the preparation of raw material mainly saline cistanche or the cynomorium songaricum of saline cistanche of the present invention or cynomorium songaricum ferment, further containing cereal, veterinary antibiotics, nut, edible mushroom, draft (other Chinese medicines) and benthophyte etc.
The saline cistanche referring to fresh saline cistanche or cynomorium songaricum, dry saline cistanche or cynomorium songaricum for saline cistanche of the present invention or cynomorium songaricum or concoct through various concocting method or cynomorium songaricum medicine materical crude slice, described saline cistanche is selected from saline cistanche, Cistanche tubulosa, halophilous herbage, husky desert cistanche and/or broomrape.Wherein fresh saline cistanche or cynomorium songaricum are cut into sheet, the strip or granular for fermentation process of suitable size after cleaning, or are polished into slurries for fermentation process.Sheet, the strip or granular for fermentation process of suitable size can be prepared into for dry saline cistanche or cynomorium songaricum or saline cistanche or cynomorium songaricum medicine materical crude slice after soaking, or be polished into slurries for fermentation process, or directly clay into power without immersion and carry out fermentation process.
For described cereal of the present invention including but not limited to wheat, rice, corn, millet, beans; Described vegetables including but not limited to leaf class, tubers, fruit class, as cucumber, muskmelon, watermelon, balsam pear, edible wild herbs, cabbage, radish, Chinese cabbage, bean sprouts, lettuce, celery etc.; Described fruit is including but not limited to orange, tomato, lemon, grape, all kinds of pears, apple, pawpaw, carambola, strawberry etc.Described nut is including but not limited to walnut, peanut, fibert, pine nut etc.Described edible mushroom is including but not limited to all kinds of mushroom.Described draft is including but not limited to matrimony vine, ginseng, aloe, Cordyceps sinensis, glossy ganoderma etc.Described benthophyte is including but not limited to sea-tangle, laver etc.Above-mentioned material uses with the form of slurries or powder.
Liquid fermentation method and solid fermentation method is had for fermentation mode of the present invention.
Liquid fermentation method:
Ferment under certain condition after fermented bacterium being inoculated in the fermentation substrate containing fluid nutrient medium.
One or more can be used in the bacterial classification of food or pharmaceutical fermentation to include but not limited to saccharomycete, lactic acid bacteria, lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus plantarum, bacillus subtilis, Bifidobacterium Bifidum, aspergillus oryzae, halimasch, Cordyceps Militaris, Ganoderma Lucidum, bright red bacterium etc. for liquid fermentation bacterial classification of the present invention.
Culture medium for liquid fermentation of the present invention is as included but not limited to malt extract medium, fruit pulp culture medium, cereal aqueous solution culture medium etc. for food or the common culture medium of medicine.Carbohydrate, nitrogenous source and inorganic salts can be added in described culture medium.Described carbohydrate includes but not limited to white sugar, brown sugar, oligosaccharides, glycitols, rock sugar, honey etc.As preferably, the addition of described carbohydrate is the 0-8% of raw material gross weight.Described nitrogenous source includes but not limited to dusty yeast, peptone, soybean cake powder, corn steep liquor, fish meal, dried silkworm chrysalis meal, wheat bran etc.As preferably, the addition of described nitrogenous source is the 0-10% of raw material gross weight.Described inorganic salts include but not limited to ammonium sulfate, manganese chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate etc.As preferably, the addition of described inorganic salts is the 0-3% of raw material gross weight.Preferably, during the fermentation, culture medium is implanted stage by stage according to the difference of bacterial classification.
Method for liquid fermentation of the present invention comprises the steps:
(1) carry out sterilization processing to raw material, sterilization processing herein comprises the bactericidal treatments that cold sterilization, radiation sterilization, chemical sterilization etc. do not destroy material quality;
(2) culture medium is prepared: adopt this area conventional method to prepare various liquid fermentation medium, include but not limited to malt extract medium, fruit pulp culture medium, cereal aqueous solution culture medium.
As preferably, described malt extract medium is that obtained dried malt forms through the upper sequence such as saccharification, gelatinization, filtration by Fructus Hordei Germinatus through screening, cleaning, dry.As preferably, described Fructus Hordei Germinatus is barley malt or wheat malt.As preferably, described drying is for drying or drying.As preferably, described saccharification adopts acid system, enzyme process or acid-enzyme binding-method saccharification.
As preferably, described fruit pulp culture medium is water intaking fruit edible portion, through cleaning, making beating (add water or do not add water), filter obtained.
As preferably, described cereal aqueous solution culture medium is by after cereal impurity elimination, cleaning, grinding, adds water boil, filtration and obtaining.
As preferably, described culture medium needs before use through sterilization treatment.
As preferably, described malt extract medium is suitable for being only fermentation substrate with Chinese medicine; Can using fruit pulp as culture medium when containing fruit in fermentation substrate; If can using the aqueous solution of cereal as culture medium when containing cereals material in fermentation substrate.This principle only does general guidance, when carbohydrate in fermentation substrate and water content enough grow for zymophyte, then fermentation substrate can be used as culture medium.
(3) prepare fermentation substrate: in sterilising medium, add saline cistanche or cynomorium songaricum raw material, preferably add the saline cistanche or cynomorium songaricum raw material that are polished into slurries.As preferably, the addition of described saline cistanche or cynomorium songaricum raw material is 0.5-8% (w/v) or the 0.5-8% (v/v) of culture medium.Preferably add other Chinese drugs powders further, further preferably add wherein one or more such as cereal, vegetables, edible mushroom, nut, benthophyte.Other Chinese medicines or cereal, vegetables, edible mushroom, nut, benthophyte can add according to conventional amount used in powder form, are generally 0-10% (w/v).
(4) bacterial classification is implanted and is fermented: after the bacterial classification of selection being carried out expansion cultivation, implant in described fermentation substrate and ferment.As preferably, described bacterial classification is anaerobic species, aerobic bacterial classification or anaerobism and aerobic bacterial classification dual-purpose.The implantation amount of described anaerobism, aerobic bacterial classification is fermentation substrate 5-16% (w/w), or 8-12% (w/w) or 10% (w/w).As preferably, described expansion cultivation comprises that solid slope is cultivated, Shaking culture and/or seed tank culture.As preferably, the culture medium composition that described solid slope is cultivated comprises glucose 2-4%, peptone 1-3%, bean powder 1-2%, agar 2-4%, magnesium sulfate 0.05-0.08%, potassium dihydrogen phosphate 0.2-0.3%, sodium chloride 0.1-0.3%.As preferably, the culture medium of described Shaking culture comprises glucose 2-3%, peptone 1-4%, bean powder 0.5-2%, wheat bran 0.5-2%, magnesium sulfate 0.01-0.1%, potassium dihydrogen phosphate 0.1-0.4%, dipotassium hydrogen phosphate 0.1-0.2%.As preferably, the rotating speed of described shaking flask is 200 turns/min.As preferably, the culture medium of described seed tank culture is beef extract 0.5%-1.5%, peptone 1%-2%, corn steep liquor 0.5%-1.0%, potassium dihydrogen phosphate 0.03%-0.05%, zinc sulfate 0.02%-0.07%, magnesium chloride 0.04%-0.08%, and all the other are water.
As preferably, when selecting anaerobic species, under anaerobic cultivate 15-40 days in 25-40 DEG C of sealing and standing, or fermentation temperature be 28-35 DEG C, 30-32 DEG C or 31 DEG C, fermentation time is 21-35 days, 25-28 days or 27 days.
Further preferably, when selecting aerobic bacteria kind, in 22-35 DEG C of fermentation 7-30 days under aeration condition, or fermentation temperature is 25-30 DEG C or 28 DEG C, and fermentation time is 14-25 days or 18-22 days or 20 days.Body of ventilating be filtrated air, throughput is 2.5-5.0L/min.
Further preferred, described fermentation is carried out stage by stage, and the first stage is aerobic fermentation, selects aerobic bacterial classification, and fermentation temperature is 22-35 DEG C, and filtrated air throughput is 2.5-5.0L/min, and the aerobic fermentation time is 7-30 days; Second stage transfers anaerobic fermentation to, selects anaerobic species, and close ventilation, control fermentation temperature is 25-40 DEG C, and the anaerobic fermentation time is 15-40 days.
(5) extract the liquid after fermentation, obtain saline cistanche or cynomorium songaricum ferment after filtering.
Solid fermentation method:
Ferment under certain condition after fermented bacterium being inoculated in the fermentation substrate containing solid medium.
One or more can be used in the bacterial classification of food or pharmaceutical fermentation to include but not limited to saccharomycete, Bacillus acidi lactici, lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus plantarum, bacillus, Bifidobacterium Bifidum, aspergillus oryzae, halimasch, Cordyceps Militaris, Ganoderma Lucidum, bright red bacterium etc. for solid fermentation bacterial classification of the present invention.
Culture medium for solid fermentation of the present invention is as included but not limited to brewer's wort solid medium, fruit culture medium, grain culture medium etc. for food or the common culture medium of medicine.Carbohydrate, nitrogenous source and inorganic salts can be added in described culture medium.Described carbohydrate includes but not limited to white sugar, brown sugar, oligosaccharides, glycitols, rock sugar, honey etc.As preferably, the addition of described carbohydrate is the 0-8% of raw material gross weight.Described nitrogenous source includes but not limited to dusty yeast, peptone, soybean cake powder, corn steep liquor, fish meal, dried silkworm chrysalis meal, wheat bran etc.As preferably, the addition of described nitrogenous source is the 0-10% of raw material gross weight.Described inorganic salts include but not limited to ammonium sulfate, manganese chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate etc.As preferably, the addition of described inorganic salts is the 0-3% of raw material gross weight.Preferably, during the fermentation, culture medium is implanted stage by stage according to the difference of bacterial classification.
Method for solid fermentation of the present invention comprises the steps:
(1) carry out sterilization processing to raw material, sterilization processing herein comprises the bactericidal treatments that cold sterilization, radiation sterilization, chemical sterilization etc. do not destroy material quality;
(2) culture medium is prepared: adopt this area conventional method to prepare various liquid fermentation medium, include but not limited to brewer's wort solid medium, fruit culture medium, grain culture medium.
As preferably, described brewer's wort solid medium is that obtained dried malt, through operations such as saccharification, gelatinization, filtrations, finally adds agar and makes by Fructus Hordei Germinatus through screening, cleaning, dry.As preferably, described Fructus Hordei Germinatus is barley malt or wheat malt.As preferably, described drying is for drying or drying.As preferably, described saccharification adopts acid system, enzyme process or acid-enzyme binding-method saccharification.As preferably, the addition of described agar is 1-5% (g/100mL), or 2-4% (g/100mL) or 3% (g/100mL).
As preferably, described fruit culture medium is water intaking fruit edible portion, through cleaning, making beating (add water or do not add water), filter, concentrated, finally add agar and obtain.As preferably, described in be concentrated into solid content in slurries be 3-15% (g/100mL) or 5-10% (g/100mL) or 8% (g/100mL).As preferably, the addition of described agar is 1-5% (g/100mL), or 2-4% (g/100mL) or 3% (g/100mL).
As preferably, described grain culture medium is by after cereal impurity elimination, cleaning, grinding, adds water boil, filtration, adds agar and obtain.As preferably, the addition of described agar is 1-5% (g/100mL), or 2-4% (g/100mL) or 3% (g/100mL).
As preferably, described culture medium needs before use through sterilization treatment.
As preferably, described brewer's wort solid medium is suitable for being only fermentation substrate with Chinese medicine; When selecting fruit culture medium containing during fruit in fermentation substrate; If can grain culture medium be selected containing during cereals material in fermentation substrate.This principle only does general guidance, when carbohydrate in fermentation substrate and water content enough grow for zymophyte, then fermentation substrate can be used as culture medium.
(3) prepare fermentation substrate: in sterilising medium, add saline cistanche or cynomorium songaricum raw material, preferably add be cut into suitable size sheet, strip or granular saline cistanche or cynomorium songaricum raw material.As preferably, the addition of described saline cistanche or cynomorium songaricum raw material is 0.5-8% (w/w) or the 1-5% (w/w) or 2.5% (w/w) of culture medium.Preferably add other Chinese drugs powders further, further preferably add wherein one or more such as cereal, vegetables, edible mushroom, nut, benthophyte.Other Chinese medicines or cereal, vegetables, edible mushroom, nut, benthophyte can add according to conventional amount used by powder form, are generally 0-10% (w/w).
(4) bacterial classification is implanted and is fermented: after the bacterial classification of selection being carried out expansion cultivation, implant in described fermentation substrate and ferment.As preferably, described bacterial classification is anaerobic species, aerobic bacterial classification or anaerobism and aerobic bacterial classification dual-purpose.The implantation amount of described anaerobism, aerobic bacterial classification is fermentation substrate 5-16% (w/w), or 8-12% (w/w) or 10% (w/w).As preferably, described expansion cultivation comprises that solid slope is cultivated, Shaking culture and/or seed tank culture.As preferably, the culture medium composition that described solid slope is cultivated comprises glucose 2-4%, peptone 1-3%, bean powder 1-2%, agar 2-4%, magnesium sulfate 0.05-0.08%, potassium dihydrogen phosphate 0.2-0.3%, sodium chloride 0.1-0.3%.As preferably, the culture medium of described Shaking culture comprises glucose 2-3%, peptone 1-4%, bean powder 0.5-2%, wheat bran 0.5-2%, magnesium sulfate 0.01-0.1%, potassium dihydrogen phosphate 0.1-0.4%, dipotassium hydrogen phosphate 0.1-0.2%.As preferably, the rotating speed of described shaking flask is 200 turns/min.As preferably, the culture medium of described seed tank culture is beef extract 0.5%-1.5%, peptone 1%-2%, corn steep liquor 0.5%-1.0%, potassium dihydrogen phosphate 0.03%-0.05%, zinc sulfate 0.02%-0.07%, magnesium chloride 0.04%-0.08%, and all the other are water.
As preferably, when selecting anaerobic species, under anaerobic cultivate 15-40 days in 25-40 DEG C of sealing and standing, or fermentation temperature be 28-35 DEG C, 30-32 DEG C or 31 DEG C, fermentation time is 21-35 days, 25-28 days or 27 days.
Further preferably, when selecting aerobic bacteria kind, in 22-35 DEG C of fermentation 7-30 days under aeration condition, or fermentation temperature is 25-30 DEG C or 28 DEG C, and fermentation time is 14-25 days or 18-22 days or 20 days.Body of ventilating be filtrated air, throughput is 2.5-5.0L/min.
Further preferred, described fermentation is carried out stage by stage, and the first stage is aerobic fermentation, selects aerobic bacterial classification, and fermentation temperature is 22-35 DEG C, and filtrated air throughput is 2.5-5.0L/min, and the aerobic fermentation time is 7-30 days; Second stage transfers anaerobic fermentation to, selects anaerobic species, and close ventilation, control fermentation temperature is 25-40 DEG C, and the anaerobic fermentation time is 15-40 days.
(5) collect the culture medium after fermentation, obtain cynomorium songaricum ferment or saline cistanche ferment.
The present invention has following advantage and effect relative to prior art:
(1) the present invention does not lose or destroys saline cistanche or cynomorium songaricum material nutrient component in preparation process, and obtained saline cistanche or cynomorium songaricum ferment significantly improve nutrition and health care value;
(2) preparation technology of the present invention is simple, and preparation process is controlled, the ferment constant product quality obtained, and can preserve by normal temperature, is suitable for large-scale production, has good market prospects;
(3) dried beef containing saline cistanche or cynomorium songaricum ferment of the present invention is applicable to the various crowd of men and women, old and young and eats, the effect that can reach develop immunitypty, memory in daily life or invigorate the kidney and stop nocturnal emission.
Detailed description of the invention
Embodiment 1
(1) get after fresh saline cistanche or cynomorium songaricum clean and be polished into slurries, cold sterilization process.
(2) preparation of malt extract medium: by barley malt through screening, cleaning, naturally dry, obtained dried malt forms through operations such as acid saccharification, gelatinization, filtrations; And add the glucose of 3%, sterilizing.
(3) prepare fermentation substrate: in above-mentioned sterilizing malt extract medium, add saline cistanche or the cynomorium songaricum slurries of step (1) gained, the addition of described saline cistanche or cynomorium songaricum slurries is 3% (v/v) of culture medium.
(4) bacterial classification is implanted and is fermented: opt lactic acid bacteria is fermented bacterium, carries out, after expansion cultivation, implanting in described fermentation substrate and fermenting through solid slope cultivation.The culture medium composition that described solid slope is cultivated comprises glucose 4%, peptone 1%, bean powder 2%, agar 4%, magnesium sulfate 0.06%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.1%.The implantation amount of described lactic acid bacteria is fermentation substrate 8% (w/w).Described fermentation is under anaerobic fermented 40 days in 30 DEG C of sealing and standing.
(5) extract the liquid after fermentation, obtain saline cistanche or cynomorium songaricum ferment after filtering.
Embodiment 2
(1) get after fresh saline cistanche or cynomorium songaricum clean and be polished into slurries, radiation sterilization process.
(2) preparation of apple pulp culture medium: after apple peel, stoning, obtains through cleaning, making beating, filtration, and sterilizing.
(3) prepare fermentation substrate: in the apple pulp culture medium of above-mentioned sterilizing, add saline cistanche or the cynomorium songaricum slurries of step (1) gained, the addition of described saline cistanche or cynomorium songaricum slurries is 2% (v/v) of culture medium.
(4) bacterial classification is implanted and is fermented: selection lactobacillus bulgaricus is fermented bacterium, after solid slope is cultivated and seed tank culture carries out expansion cultivation, implants in described fermentation substrate and ferments.The culture medium composition that described solid slope is cultivated comprises glucose 4%, peptone 1%, bean powder 2%, agar 4%, magnesium sulfate 0.06%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.1%.The culture medium of described seed tank culture is beef extract 0.8%, peptone 2%, corn steep liquor 0.5%, potassium dihydrogen phosphate 0.05%, zinc sulfate 0.05%, magnesium chloride 0.06%, and all the other are water.The implantation amount of described lactobacillus bulgaricus is fermentation substrate 10% (w/w).Described fermentation is under anaerobic fermented 25 days in 28 DEG C of sealing and standing.
(5) extract the liquid after fermentation, obtain saline cistanche or cynomorium songaricum ferment after filtering.
Embodiment 3
(1) get after fresh saline cistanche or cynomorium songaricum clean and be polished into slurries, chemical sterilization process.
(2) preparation of cereal aqueous solution culture medium: after corn impurity elimination, cleaning, grinding, after adding water boil, filtering, adds the glucose of 5%, sterilizing and obtaining.
(3) prepare fermentation substrate: in the cereal aqueous solution culture medium of above-mentioned sterilizing, add saline cistanche or the cynomorium songaricum slurries of step (1) gained, the addition of described saline cistanche or cynomorium songaricum slurries is 5% (v/v) of culture medium.
(4) bacterial classification is implanted and is fermented: selection lactobacillus acidophilus is fermented bacterium, carries out, after expansion cultivation, implanting in described fermentation substrate and fermenting through Shaking culture and seed tank culture.The culture medium composition of described Shaking culture comprises glucose 2%, peptone 2%, bean powder 1%, wheat bran 2%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.2%.The culture medium of described seed tank culture is beef extract 0.5%, peptone 1%, corn steep liquor 0.5%, potassium dihydrogen phosphate 0.03%, zinc sulfate 0.03%, magnesium chloride 0.05%, and all the other are water.The implantation amount of described lactobacillus acidophilus is fermentation substrate 12% (w/w).Described fermentation is under anaerobic fermented 28 days in 32 DEG C of sealing and standing.
(5) extract the liquid after fermentation, obtain saline cistanche or cynomorium songaricum ferment after filtering.
Embodiment 4
(1) saline cistanche or the cynomorium songaricum of getting drying are thinly sliced, are pulverized, cold sterilization process.
(2) preparation of brewer's wort solid medium: by wheat malt through screening, cleaning, oven dry, obtained dried malt is through operations such as enzyme process saccharification, gelatinization, filtrations, and the agar finally adding 3% (g/100mL) is made; And add the glucose of 3%, sterilization treatment.
(3) prepare fermentation substrate: in above-mentioned sterilizing brewer's wort solid medium, add saline cistanche or the cynomorium songaricum raw material of step (1) gained, the addition of described saline cistanche or cynomorium songaricum raw material is 2.5% (w/w) of culture medium.
(4) bacterial classification is implanted and is fermented: selection bacillus subtilis is fermented bacterium, carries out, after expansion cultivation, implanting in described fermentation substrate and fermenting through solid slope cultivation.The culture medium composition that described solid slope is cultivated comprises glucose 2%, peptone 2%, bean powder 2%, agar 3%, magnesium sulfate 0.06%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.1%.The implantation amount of described bacillus subtilis is fermentation substrate 10% (w/w).Described fermentation be under aeration condition in 25 DEG C fermentation 18 days, body of ventilating be filtrated air, throughput is 5.0L/min.
(5) collect the culture medium after fermentation, obtain cynomorium songaricum ferment or saline cistanche ferment.
Embodiment 5
(1) saline cistanche or the cynomorium songaricum of getting drying are cut into strip, pulverizing, radiation sterilization process.
(2) preparation of fruit culture medium: after getting carambola stoning, peeling, through cleaning, making beating, filter, being concentrated into solid content in slurries is 3% (g/100mL), and the agar adding 2% (g/100mL) obtains; Rear sterilization treatment.
(3) prepare fermentation substrate: in above-mentioned sterilizing fruit culture medium, add saline cistanche or the cynomorium songaricum raw material of step (1) gained, the addition of described saline cistanche or cynomorium songaricum raw material is 3% (w/w) of culture medium.
(4) bacterial classification is implanted and is fermented: selection saccharomycete is fermented bacterium, carries out, after expansion cultivation, implanting in described fermentation substrate and fermenting through Shaking culture.The culture medium composition of described Shaking culture comprises glucose 3%, peptone 4%, bean powder 0.5%, wheat bran 0.5%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.4%, dipotassium hydrogen phosphate 0.15%.The rotating speed of described shaking flask is 200 turns/min.Described saccharomycetic implantation amount is fermentation substrate 9% (w/w).Described fermentation be under aeration condition in 30 DEG C fermentation 21 days, body of ventilating be filtrated air, throughput is 3.0L/min.
(5) collect the culture medium after fermentation, obtain cynomorium songaricum ferment or saline cistanche ferment.
Embodiment 6
(1) saline cistanche or the cynomorium songaricum of getting drying are cut into granular, pulverizing, chemical sterilization process.
(2) preparation of grain culture medium: after millet impurity elimination, cleaning, grinding, adds water boil, filtration, adds 3% (g/100mL) agar to obtaining; After add 3% glucose, sterilization treatment.
(3) prepare fermentation substrate: in above-mentioned sterilizing grain culture medium, add saline cistanche or the cynomorium songaricum raw material of step (1) gained, the addition of described saline cistanche or cynomorium songaricum raw material is 2.5% (w/w) of culture medium.
(4) bacterial classification is implanted and is fermented: selection aspergillus oryzae is fermented bacterium, carries out, after expansion cultivation, implanting in described fermentation substrate and fermenting through Shaking culture and seed tank culture.The culture medium composition of described Shaking culture comprises glucose 2%, peptone 3%, bean powder 1%, wheat bran 1%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.4%, dipotassium hydrogen phosphate 0.2%.The rotating speed of described shaking flask is 200 turns/min.The culture medium of described seed tank culture is beef extract 1.5%, peptone 1.5%, corn steep liquor 1.0%, potassium dihydrogen phosphate 0.05%, zinc sulfate 0.02%, magnesium chloride 0.04%, and all the other are water.Described saccharomycetic implantation amount is fermentation substrate 10% (w/w).Described fermentation be under aeration condition in 35 DEG C fermentation 20 days, body of ventilating be filtrated air, throughput is 2.5L/min.
(5) collect the culture medium after fermentation, obtain cynomorium songaricum ferment or saline cistanche ferment.
Embodiment 7
Embodiment of the present invention 1-6 gained saline cistanche or cynomorium songaricum ferment reference standard are analyzed, total arsenic≤0.2mg/L; Lead≤0.05mg/L; Copper≤5mg/L, total bacterium colony≤100cfu/mL.Analysis result through sensory evaluation is known, and embodiment of the present invention 1-6 prepares good mouthfeel, liquid clarifies bright saline cistanche or cynomorium songaricum ferment.And above-mentioned preparation process is controlled, fermented product steady quality, can preserve by normal temperature, is suitable for large-scale production, has good market prospects.
Embodiment 8
The preparation of beef gruel: boned by beef, clean, drain, puts into cutmixer by raw meat and cuts into meat gruel, and adds flavoring mixing, mixes 8h under 4 DEG C of conditions;
Die-filling boiling: the beef gruel of mixing loaded in grinding tool, builds and boils 3h in the pot being placed in 90 DEG C;
Cooling, section, vegetable oil smear;
Baking: toast 2h at sliced meat being placed in 80 DEG C;
After dried beef slices after baking is placed in saline cistanche or cynomorium songaricum ferment infuse 2h prepared by embodiment 1, air-dry or dry, obtained the present invention contains the dried beef of saline cistanche or cynomorium songaricum ferment.
Embodiment 9
Preparation saline cistanche or cynomorium songaricum ferment powder: the saline cistanche of prepare embodiment 6 or cynomorium songaricum ferment are dried and made powder, add as required or not with flavoring, mix;
Select the forward and backward das Beinfleisch of ox, reject fat and muscle tendon etc., clean, drain, be cut into the cube meat of 0.3kg;
Precook: cube meat is put into pot, clear water is boiled, and boils 30min, picks up cooling, and former soup is stand-by;
Stripping and slicing: cut into inch strips by different size, in the same size, thickness is even;
Halogen boils: the cutlet cut is put into former soup, adds flavoring, first simmers with moderate heat with after big fire boiling, finally receives juice with slow fire, treats that thick gravy is received dry, can be off the pot;
4h is toasted at 60 DEG C; Mix with saline cistanche or cynomorium songaricum ferment powder, make the outer uniform adhesion ferment powder of dried beef, obtained the present invention contains the dried beef of saline cistanche or cynomorium songaricum ferment.
Embodiment 10
Give different volunteer by the dried beef containing saline cistanche or cynomorium songaricum ferment of embodiment of the present invention 8-9 gained to take, every day 100g, after taking one or two moon continuously, all volunteers reflect health status, the state of mind all has clear improvement, immunity and memory strengthen all to some extent, or reach the effect of invigorating the kidney and stopping nocturnal emission.
Although embodiment of the present invention are open as above, but it is not restricted to listed in description and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment described.
Claims (9)
1. contain a dried beef for saline cistanche or cynomorium songaricum ferment, it is characterized in that, the content of described saline cistanche or cynomorium songaricum ferment is 0.5-8%.
2. dried beef according to claim 1, is characterized in that, the described dried beef containing saline cistanche or cynomorium songaricum ferment is by after adopting the obtained dried beef of conventional method to be placed in saline cistanche or cynomorium songaricum ferment infuse, air-dry or dry and obtain; Or the dried beef adopting conventional method to obtain is mixed with saline cistanche or cynomorium songaricum ferment powder or mixes with the saline cistanche or cynomorium songaricum ferment powder that contain flavoring, make the outer uniform adhesion ferment powder of dried beef and obtain.
3. dried beef according to claim 1, is characterized in that, described in have saline cistanche or cynomorium songaricum ferment with saline cistanche or cynomorium songaricum for primary raw material, obtain through fermentation.
4. dried beef according to claim 3, it is characterized in that, described saline cistanche or cynomorium songaricum refer to fresh saline cistanche or cynomorium songaricum, dry saline cistanche or cynomorium songaricum or the saline cistanche of concocting through various concocting method or cynomorium songaricum medicine materical crude slice, are selected from saline cistanche, Cistanche tubulosa, halophilous herbage, husky desert cistanche or broomrape.
5. dried beef according to claim 3, is characterized in that, the mode of described fermentation is selected from liquid fermentation method and solid fermentation method.
6. dried beef according to claim 3, is characterized in that, described liquid fermentation method ferments under certain condition after fermented bacterium being inoculated in the fermentation substrate containing fluid nutrient medium; Described solid fermentation method ferments under certain condition after fermented bacterium being inoculated in the fermentation substrate containing solid medium.
7. dried beef according to claim 6, it is characterized in that, described fermented bacterium be selected from saccharomycete, lactic acid bacteria, lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus plantarum, bacillus subtilis, Bifidobacterium Bifidum, aspergillus oryzae, halimasch, Cordyceps Militaris, Ganoderma Lucidum, bright red bacterium one or more.
8. dried beef according to claim 3, is characterized in that, described liquid fermentation method comprises the steps:
(1) carry out sterilization processing to raw material, described sterilization is selected from cold sterilization, radiation sterilization or chemical sterilization;
(2) culture medium is prepared: adopt this area conventional method to prepare malt extract medium, fruit pulp culture medium or cereal aqueous solution culture medium;
(3) prepare fermentation substrate: in sterilising medium, add saline cistanche or cynomorium songaricum raw material;
(4) bacterial classification is implanted and is fermented: after the bacterial classification of selection being carried out expansion cultivation, implant in described fermentation substrate and ferment;
(5) extract the liquid after fermentation, obtain saline cistanche or cynomorium songaricum ferment after filtering.
9. dried beef according to claim 3, is characterized in that, described solid fermentation method comprises the steps:
(1) carry out sterilization processing to raw material, described sterilization is selected from cold sterilization, radiation sterilization or chemical sterilization;
(2) culture medium is prepared: adopt this area conventional method to prepare brewer's wort solid medium, fruit culture medium or grain culture medium;
(3) prepare fermentation substrate: in sterilising medium, add saline cistanche or cynomorium songaricum raw material;
(4) bacterial classification is implanted and is fermented: after the bacterial classification of selection being carried out expansion cultivation, implant in described fermentation substrate and ferment;
(5) extract the liquid after fermentation, obtain saline cistanche or cynomorium songaricum ferment after filtering.
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