CN104849335A - Method for detecting ionic calcium content of blood sample - Google Patents
Method for detecting ionic calcium content of blood sample Download PDFInfo
- Publication number
- CN104849335A CN104849335A CN201510289961.3A CN201510289961A CN104849335A CN 104849335 A CN104849335 A CN 104849335A CN 201510289961 A CN201510289961 A CN 201510289961A CN 104849335 A CN104849335 A CN 104849335A
- Authority
- CN
- China
- Prior art keywords
- calcium
- calcium ion
- electrode
- blood sample
- response value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a method for detecting the ionic calcium content of a blood sample, and belongs to the field of calcium ion detection. The method comprises the following steps: by adopting polytetrafluoroethylene, synthetic plant ester, sodium tetraphenylborate, laurate, N-butyl pyridine calcium hexafluorophosphate and N,N,N',N'-tetracyclohexyl-3-oxa-glutaramide as solutes, reacting with a solvent tetrahydrofuran to prepare a calcium ion sensing membrane, and filling the calcium ion sensing membrane on a glassy carbon electrode substrate to obtain a calcium ion selective electrode; and measuring the ionic calcium content of the blood sample by using an ion selective electrode method which is used for a self-made calcium ion selective electrode. The method disclosed by the invention can be used for rapidly measuring the calcium ion content of the blood sample, and is high in measurement accuracy, good in stability, high in specificity, simple in measurement step and low in test cost.
Description
Technical field
The present invention relates to calcium ion detection field, specifically a kind of method detecting blood sample calcium ion content.
Background technology
Blood calcium is almost all present in blood plasma, can be divided into diffusion calcium and the large class of nondiffusible calcium two.Nondiffusible calcium refers to and the calcium that protein (mainly albumin) combines, and accounts for 40% of the total calcium of blood plasma.60% is diffusible calcium.This two classes calcium exists with three kinds of forms: 1. diffusive ionic calcium, for having the part of physiologically active.2. diffusivity nonionic calcium (accounting for the l5% of serum total calcium amount) is for be combined into composite calcium with citric acid, heavy carbonic root.3. nondiffusible calcium is the calcium be combined with plasma albumin, its content is relevant with density of plasma albumin, this part calcium is not easily through vascular wall, also without physiologically active, as plasma albumin obviously declines, non-dispersive calcium also reduces, so that serum total calcium amount declines, but because free calcium does not reduce, so there is not calcium deficiency symptom clinically.Non-dispersive calcium and diffusivity nonionic calcium are associativity calcium, can change mutually between non-dispersive calcium and ionized calcium, and in mobile equilibrium, when free calcium concentration reduces, non-dispersive calcium can be released into free calcium gradually.The assay method of blood calcium is a lot, and total calcium generally can be divided into measure and ionized calcium determination method.The former comprises isotope dilution mass spectrometry (isotope diluting mass spectrometry), atomic absorption spectrography (AAS) (atomicabsorption spectroscopy, AAS), spectrophotometric method (spectrophotography) and compleximetry (chelating titration) etc.AAS is the reference method of the most frequently used Accurate Determining blood plasma total calcium content, but somewhat expensive, be unsuitable for routine work; In spectrophotometric method with methyl thymol blue colourimetry (methyl thymol blue colorimetry) and arsenazo Ⅲ colourimetry (Arsenazo III colorimetry) the most frequently used.Compleximetry is simple and easy to do, but judges that terminal affects by subjective factor, and be eliminated trend, but many laboratories still adopt.International Federation of Clinical Chemistry (IFCC) recommends isotope dilution mass spectrometry (IDMS) for decisive method, and atomic absorption spectrophotometry is reference method.The common laboratory conventional determining method that the World Health Organization (WHO) (WHO) is recommended is o-cresolphthalein complex copper method (OCPC).Ion selective electrode method measures ionized calcium in clinical practice, although determination of electrode process is without chemical contamination, quick and convenient, relatively additive method stability and specificity poor.CN102735736B discloses a kind of sense film for calcium ion-selective electrode, and the selectivity of this film has much room for improvement, and its ion selectivity carrier is laurate.Patent of invention CN102507696B discloses a kind of ion-selective electrode based on Graphene electrodes, comprise electrode matrix and the ion selectivity polymer film being coated in electrode matrix surface, described electrode matrix is doped with Graphene or the electrode being had graphene film by electro-deposition at electrode face finish in electrode.
Summary of the invention
The present invention is directed to prior art exist deficiency, a kind of method detecting blood sample calcium ion content is provided, the method is quick and convenient, stability and specificity good.
In order to achieve the above object, the present invention is achieved by the following technical solutions:
Detect a method for blood sample calcium ion content, concrete steps comprise:
(1) make calcium ion-selective electrode by oneself: by teflon 33-35% by mass percentage, synthesis vegetable esters 62-65%, tetraphenylboron sodium 0.5-1.0%, laurate 1-1.5%, N-butyl-pyridinium hexafluorophosphoric acid calcium 1-1.5%, N, N, N ', N '-four cyclohexyl-3-oxa-glutaramide 0-0.5% is as solute, with solvents tetrahydrofurane mixing and stirring, wherein solvent quality is 4-8 times of solute, mix and blend 16h at normal temperatures, then after volatilization 60h, obtained thickness is 0.4-0.6mm sense film, sense film is loaded on glass-carbon electrode matrix, obtain calcium ion-selective electrode,
(2) prepare calcium ion standard serial solution, at room temperature, two electrode systems are set up by Calcium signaling and contrast electrode, specifically comprise indicator electrode and saturated calomel electrode, Calcium signaling is connected on indicator electrode, saturated calomel electrode does contrast electrode, measure the potential response value of calcium ion in the measurement liquid containing sodium chloride and Tris-NaAc buffer solution of variable concentrations, draw concentration-potential response value working curve, in measurement liquid, the volumetric molar concentration of sodium chloride is the pH=7.4 of 0.10mol/L, Tris-NaAc buffer solution;
(3) measure the potential response value of blood sample calcium ion to be measured, potential response value is substituted into concentration-potential response value working curve, obtains blood sample calcium ion concentration to be measured.
Compared with prior art, beneficial effect of the present invention: energy Quick Measuring goes out the calcium ion content in blood sample, and measuring accuracy is high, good stability, and specificity is high, and measuring process is simple, and testing cost is low.
Embodiment
Embodiment is used for further illustrating the present invention below, but does not limit the content that the present invention relates in any form.
Embodiment
Detect a method for blood sample calcium ion content, concrete steps comprise:
(1) make calcium ion-selective electrode by oneself: by teflon 33.5% by mass percentage, synthesis vegetable esters 63%, tetraphenylboron sodium 0.8%, laurate 1.2%, N-butyl-pyridinium hexafluorophosphoric acid calcium 1.2%, N, N, N ', N '-four cyclohexyl-3-oxa-glutaramide 0.3% is as solute, with solvents tetrahydrofurane mixing and stirring, wherein solvent quality is 6 times of solute, mix and blend 16h at normal temperatures, then after volatilization 60h, obtained thickness is 0.5mm sense film, sense film is loaded on glass-carbon electrode matrix, obtain calcium ion-selective electrode;
(2) prepare calcium ion standard serial solution, at room temperature, two electrode systems are set up by Calcium signaling and contrast electrode, specifically comprise indicator electrode and saturated calomel electrode, Calcium signaling is connected on indicator electrode, saturated calomel electrode does contrast electrode, measure the potential response value of calcium ion in the measurement liquid containing sodium chloride and Tris-NaAc buffer solution of variable concentrations, draw concentration-potential response value working curve, in measurement liquid, the volumetric molar concentration of sodium chloride is the pH=7.4 of 0.10mol/L, Tris-NaAc buffer solution;
(3) measure the potential response value of blood sample calcium ion to be measured, potential response value is substituted into concentration-potential response value working curve, obtains blood sample calcium ion concentration to be measured.
After measured, the response time that the electrode potential of the Calcium signaling of the present embodiment reaches balance and stability is no more than 25s, and wherein calcium ion is 1.00 × 10 in volumetric molar concentration
-5-1.00 × 10
-2presenting in the scope of mol/L can this special response.
Carry out 20 repeatability by the method for embodiment to the human normal plasma without any acute and chronic diseases to detect, result shows, blood sample ionized calcium average out to 1.18mmol/L.
Carry out recovery testu, recording average recovery rate is 99.6%.
Above-described embodiment is preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (1)
1. detect a method for blood sample calcium ion content, it is characterized in that: concrete steps comprise:
(1) make calcium ion-selective electrode by oneself: by teflon 33-35% by mass percentage, synthesis vegetable esters 62-65%, tetraphenylboron sodium 0.5-1.0%, laurate 1-1.5%, N-butyl-pyridinium hexafluorophosphoric acid calcium 1-1.5%, N, N, N ', N '-four cyclohexyl-3-oxa-glutaramide 0-0.5% is as solute, with solvents tetrahydrofurane mixing and stirring, wherein solvent quality is 4-8 times of solute, mix and blend 16h at normal temperatures, then after volatilization 60h, obtained thickness is 0.4-0.6mm sense film, sense film is loaded on glass-carbon electrode matrix, obtain calcium ion-selective electrode,
(2) prepare calcium ion standard serial solution, at room temperature, two electrode systems are set up by Calcium signaling and contrast electrode, specifically comprise indicator electrode and saturated calomel electrode, Calcium signaling is connected on indicator electrode, saturated calomel electrode does contrast electrode, measure the potential response value of calcium ion in the measurement liquid containing sodium chloride and Tris-NaAc buffer solution of variable concentrations, draw concentration-potential response value working curve, in measurement liquid, the volumetric molar concentration of sodium chloride is the pH=7.4 of 0.10mol/L, Tris-NaAc buffer solution;
(3) measure the potential response value of blood sample calcium ion to be measured, potential response value is substituted into concentration-potential response value working curve, obtains blood sample calcium ion concentration to be measured.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510289961.3A CN104849335A (en) | 2015-05-29 | 2015-05-29 | Method for detecting ionic calcium content of blood sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510289961.3A CN104849335A (en) | 2015-05-29 | 2015-05-29 | Method for detecting ionic calcium content of blood sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104849335A true CN104849335A (en) | 2015-08-19 |
Family
ID=53849138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510289961.3A Pending CN104849335A (en) | 2015-05-29 | 2015-05-29 | Method for detecting ionic calcium content of blood sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104849335A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105334255A (en) * | 2015-10-23 | 2016-02-17 | 吴国清 | Calcium ion selective membrane and preparation method thereof |
| CN110734583A (en) * | 2019-09-05 | 2020-01-31 | 北京华科仪科技股份有限公司 | Induction film of calcium ion selective electrodes and preparation method thereof |
| RU2736488C1 (en) * | 2020-03-19 | 2020-11-17 | Федеральное государственное бюджетное учреждение науки Институт общей и неорганической химии им. Н.С. Курнакова Российской академии наук (ИОНХ РАН) | Composition of ion-selective electrode membrane for determination of calcium ions |
| CN114235928A (en) * | 2021-12-09 | 2022-03-25 | 铂恩医疗科技(深圳)有限公司 | Calcium ion selective electrode induction membrane solution, preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5863972A (en) * | 1994-11-14 | 1999-01-26 | Chiron Diagnostics Corporation | Randomly segmented thermoplastic polyurethanes as matrix for electrochemical analysis of Ca++ ions |
| CN102507696A (en) * | 2011-10-19 | 2012-06-20 | 浙江大学 | Ion-selective electrode based on graphene electrode and preparation method thereof |
| CN102735736A (en) * | 2012-07-16 | 2012-10-17 | 上海惠中医疗科技有限公司 | Induction film used for calcium ion-selective electrode, production method and application thereof |
| CN102980931A (en) * | 2012-11-23 | 2013-03-20 | 浙江大学 | All-solid-state selective electrode based on functional ion liquid and preparation method of all-solid-state selective electrode |
| WO2013081442A1 (en) * | 2011-12-01 | 2013-06-06 | Mimos Berhad | Calcium sensing device and method of preparing thereof |
| CN103424450A (en) * | 2013-08-26 | 2013-12-04 | 深圳市希莱恒医用电子有限公司 | Ion selective electrode based on conducting polymer film and preparation method thereof |
-
2015
- 2015-05-29 CN CN201510289961.3A patent/CN104849335A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5863972A (en) * | 1994-11-14 | 1999-01-26 | Chiron Diagnostics Corporation | Randomly segmented thermoplastic polyurethanes as matrix for electrochemical analysis of Ca++ ions |
| CN102507696A (en) * | 2011-10-19 | 2012-06-20 | 浙江大学 | Ion-selective electrode based on graphene electrode and preparation method thereof |
| WO2013081442A1 (en) * | 2011-12-01 | 2013-06-06 | Mimos Berhad | Calcium sensing device and method of preparing thereof |
| CN102735736A (en) * | 2012-07-16 | 2012-10-17 | 上海惠中医疗科技有限公司 | Induction film used for calcium ion-selective electrode, production method and application thereof |
| CN102980931A (en) * | 2012-11-23 | 2013-03-20 | 浙江大学 | All-solid-state selective electrode based on functional ion liquid and preparation method of all-solid-state selective electrode |
| CN103424450A (en) * | 2013-08-26 | 2013-12-04 | 深圳市希莱恒医用电子有限公司 | Ion selective electrode based on conducting polymer film and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105334255A (en) * | 2015-10-23 | 2016-02-17 | 吴国清 | Calcium ion selective membrane and preparation method thereof |
| CN110734583A (en) * | 2019-09-05 | 2020-01-31 | 北京华科仪科技股份有限公司 | Induction film of calcium ion selective electrodes and preparation method thereof |
| RU2736488C1 (en) * | 2020-03-19 | 2020-11-17 | Федеральное государственное бюджетное учреждение науки Институт общей и неорганической химии им. Н.С. Курнакова Российской академии наук (ИОНХ РАН) | Composition of ion-selective electrode membrane for determination of calcium ions |
| CN114235928A (en) * | 2021-12-09 | 2022-03-25 | 铂恩医疗科技(深圳)有限公司 | Calcium ion selective electrode induction membrane solution, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yiping et al. | Ion chromatography for rapid and sensitive determination of fluoride in milk after headspace single-drop microextraction with in situ generation of volatile hydrogen fluoride | |
| US8093059B2 (en) | Method for simply quantitatively determining hexavalent chromium technical field | |
| CN104849335A (en) | Method for detecting ionic calcium content of blood sample | |
| CN101692093A (en) | Automatic analyzer for anionic surfactant in water and automatic analysis method | |
| CN102539426A (en) | Method for determining phosphorus in silicon-manganese alloy | |
| CN106153498A (en) | A kind of new method detecting solute concentration in solution and device thereof | |
| CN103293175B (en) | Measure the method for liquid soluble glass chemical composition | |
| CN106324185A (en) | Measuring method for acid value of unsaturated polyester resin emulsion | |
| CN114184710A (en) | Method for detecting content of lithium hexafluorophosphate in lithium ion battery electrolyte | |
| CN102621135A (en) | Method for detecting trace chloride ions in boiler water | |
| CN102866197B (en) | Method for quickly detecting concentrations of various electrolytes by ion-selective electrodes | |
| CN105572290B (en) | Single biphosphonate and AEO detection method of content in phosphate | |
| Borges et al. | Constant-current coulometry and ion chromatography bromide determination to characterize the purity of the potassium chloride | |
| CN103175930B (en) | A kind of HPLC analytical method measuring sodium sulphite content | |
| CN103698370A (en) | New metformin hydrochloride detection method | |
| CN110361435B (en) | Ionic liquid modified montmorillonite modified glassy carbon electrode and preparation method and application thereof | |
| CN102866190B (en) | Magnesium ion-selective electrode | |
| CN102323232B (en) | Method for testing content of potassium | |
| Gomes et al. | The quantification of sodium in mineral waters using a quartz crystal microbalance | |
| CN111443150A (en) | Method for detecting content of acetylcysteine and acetyltyrosine in compound amino acid injection | |
| CN104297379B (en) | The method of high performance liquid chromatography-internal mark method determination pyrogallic acid content | |
| CN109001340A (en) | A kind of rapid assay methods of low concentration salt flushing liquor multicomponent content | |
| CN104569208B (en) | A kind of HPLC analytical method of serine | |
| CN106526050B (en) | The content assaying method of phenol in a kind of iron dextran injection | |
| CN115453022B (en) | Method for rapidly judging formaldehyde content in white spirit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150819 |
|
| RJ01 | Rejection of invention patent application after publication |