CN102735736A - Induction film used for calcium ion-selective electrode, production method and application thereof - Google Patents
Induction film used for calcium ion-selective electrode, production method and application thereof Download PDFInfo
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- CN102735736A CN102735736A CN2012102447311A CN201210244731A CN102735736A CN 102735736 A CN102735736 A CN 102735736A CN 2012102447311 A CN2012102447311 A CN 2012102447311A CN 201210244731 A CN201210244731 A CN 201210244731A CN 102735736 A CN102735736 A CN 102735736A
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Abstract
The invention relates to the technical field of an induction film used for a calcium ion-selective electrode, a production method and application thereof. The method comprises the following steps: taking polymer, plasticizer, ion selective carrier and fat soluble salt as solute, and uniformly mixing the solute with solvent and stirring for 12 hours at normal temperature, wherein the total content of the solute is 150-400mg/ml; and then volatilizing for 72 hours to prepare the induction film with thickness of about 0.5mm, wherein the polymer is one or more of carboxyl polyvinyl chloride, polyvinyl chloride with high molecular weight or polyurethane, the plasticizer is one or two of bis(2-ethylhexyl) sebacate and o-nitrophenyl octyl ether, the ion selective carrier is laurate, the fat soluble salt is potassium tetra-(4-chlorphenyl) boric acid, and the solvent is tetrahydrofuran or a mixture of the tetrahydrofuran and acetone.
Description
Technical field
Patent of the present invention relates to and is used for calcium ion-selective electrode sense film, working method and application thereof.
Background technology
The rescue of the development of modern medicine, particularly clinical urgent patient need be tested to the electrolyte of human body, and calcium ion is important test item wherein.Calcium is relevant with growth, the skeleton development of human body, has also played the part of the key player in blood clotting.And keep calcium (ion) content of normal concentration in the serum, just can keep normal heart and vascular health.Calcium has been played the part of very important role with intercellular citation in cell, so each intracellular calcium ion concentration all will pass through strict control.And more special in the cell of muscle, except storing mode, calcium is also quite important to starting contraction of muscle.Other are like lipid-metabolism etc., and are also extremely important.
Calcium in the blood plasma has 48% for ionic species, and calcium ion has special and significant effects to nerve fiber, if the blood calcium ion concentration descends, nerve fiber can be overexcited, only cause hand/foot or even tic of limbs; On the other hand, hypercalcemia suppresses nervous excitation.The concentration quite stable of blood calcium by the accurate control of parathormone, is among the balance bone calcium and blood calcium.Blood calcium concentration is low, is then replenished by bone calcium; Otherwise blood calcium concentration is high, then calcium is deposited in the bone to store, or excretes in urine through kidney.
Detect Ca ion concentration employing Ca ISE in the blood at present, core component is exactly the calcium ion sense film.And the calcium ion sense film all adopts the form film forming of dripping film at present, and directly the film drop with preparation is added to the film forming of volatilizing in the electrode chambers.This mode need repeatedly drip film liquid (10-15 time), drips once more after each film liquid fully volatilizees, and relatively wastes time and energy, and harmfulness is bigger.And directly be added drop-wise in the electrode chambers; The solute of film liquid is most of or all be tetrahydrofuran; Can corrode all plastic products, some adjuvant can be dissolved in the film liquid in the electrode inner chamber pmma material, like siliceous filler; Can destroy sense film calcium ion is adsorbed, influence the sense film performance.
Summary of the invention
The purpose of this invention is to provide a kind of sense film, working method and application thereof that is used for calcium ion-selective electrode.
In order to achieve the above object, technical scheme of the present invention is following:
A kind of calcium ion-selective electrode sense film that is used for, even with solute and one or more solvent, film forming after the solvent evaporates; Said solute is made up of the plastifier of 20-40% polymkeric substance, 50-75%, the ion selectivity carrier of 1-8% and the liposoluble salt of 0.5-3%, all calculates with the percentage by weight that accounts for the solute general assembly (TW), and the total content of solute is 150-400mg/ml;
Described polymkeric substance is the carboxyl PVC, high molecular weight pvc, or one or more of polyurethane;
Described plastifier is one or both of two-(2-ethylhexyl) last of the ten Heavenly stems two, ortho-nitrophenyl octyl ether;
Described ion selectivity carrier is a laurate;
Described liposoluble salt is potassium four (4-chlorphenyl) boric acid;
Described solvent is the potpourri of tetrahydrofuran or tetrahydrofuran, acetone.
A kind of sense film working method that is used for calcium ion-selective electrode; Stir as solute and solvent with above-mentioned polymkeric substance, plastifier, ion selectivity carrier, liposoluble salt; Wherein the total content of solute is 150-400mg/ml; Mix at normal temperatures and stirred 12 hours, producing after volatilizing then 72 hours and making thickness is 0.5mm left and right sides sense film.
A kind of application that is used for the sense film of calcium ion-selective electrode is applied to measure the block sense film ion-selective electrode of electrolytic calcium ion test ion.
Calcium ion-selective electrode sense film and the working method thereof of being used for provided by the invention, technique effect is following:
(1) sense film stable performance can be at the clinical potassium concentration of accurately stably testing;
(2) process film in advance, make electrode sense film performance consistent;
(3) independent film forming outside electrode chambers, solvent can not contact with the material of electrode chambers, do not have the objectionable impurities stripping;
(4) once process sense film, avoided repeatedly dripping in the traditional fabrication process film and waited for solvent evaporates.Improved work efficiency, solvent evaporates reduces the human harm number of times during dropping.
Embodiment
Embodiment 1 ~ 7:
A kind of sense film that is used for calcium ion-selective electrode, even with solute and one or more solvent, film forming after the solvent evaporates; Said solute is made up of the plastifier of 20-40% polymkeric substance, 50-75%, the ion selectivity carrier of 1-8% and the liposoluble salt of 0.5-3%, all calculates with the percentage by weight that accounts for the solute general assembly (TW), and the total content of solute is 150-400mg/ml;
Described polymkeric substance is the carboxyl PVC, high molecular weight pvc, or one or more of polyurethane;
Described plastifier is one or both of two-(2-ethylhexyl) last of the ten Heavenly stems two, ortho-nitrophenyl octyl ether;
Described ion selectivity carrier is a laurate;
Described liposoluble salt is potassium four (4-chlorphenyl) boric acid;
Described solvent is the potpourri of tetrahydrofuran or tetrahydrofuran, acetone.
Below be the concrete steps of working method:
(1) under the room temperature fuming cupboard, solvent is added in the potpourri of solute, add clean magneton, the bottle mouth is sealed with sealing film; Place agitating solution on the magnetic stirrer; Stir speed (S.S.) is 500-800r/min, and mixing time is 12 hours, and each solute and solvent are stirred;
(2) solution that stirs is moved in the smooth clean stainless steel film container with the glass liquid-transfering gun,, make the film fluid power volatilize enough slowly and keep clean at cloche of container outer mask; .
(3) in the solution solvent evaporates after 72 hours made film be the calcium ion sense film, thickness is about 0.5mm.
The following calcium ion sense film set of dispense that is used for for the embodiment of the invention 1 ~ 7 compares:
Wherein polymer P VC-COOH is the carboxyl PVC, and HMW-PVC is a high molecular weight pvc;
Wherein plastifier DOS was two-(2-ethylhexyl) last of the ten Heavenly stems two, and NPOE is the ortho-nitrophenyl octyl ether;
Wherein the ion selectivity carrier is a laurate;
Wherein liposoluble salt KTpCIPB is potassium four (4-chlorphenyl) boric acid;
Wherein solvent THF is a tetrahydrofuran, or the potpourri of tetrahydrofuran, acetone.
In embodiment 1-7, take by weighing solute according to said ratio and be dissolved in preparation sense film liquid in the solvent, under the room temperature fuming cupboard, solvent is added in the potpourri of solute; Add clean magneton; With sealing film the bottle mouth is sealed, placed agitating solution on the magnetic stirrer, mixing time is 12 hours; Stir speed (S.S.) is 500-800r/min, and each solute and solvent are stirred; The solution that stirs is moved in the smooth clean stainless steel film container with the glass liquid-transfering gun,, make the film fluid power volatilize enough slowly and keep clean at cloche of container outer mask; In the solution solvent evaporates after 72 hours made film be the calcium ion sense film.
The above-mentioned calcium ion sense film that is prepared from packed into process the block sense film ion-selective electrode of measuring the electrolytic calcium ion test ion in the electrode; These electrodes are carried out slope, precision, accuracy, stability test; Slope is all near this special slope of ability; Other performance test can both be passed through the rower performance requirement, and is as shown in the table:
The foregoing description 1 to embodiment 7 electrode is carried out life-span examination, advance whole blood every day 100 times, it is cleaned after having advanced blood sample.Can both surpass 30 days by sample introduction with top electrode, the life-span all surpasses more than 3000 times.
Reaction film of the present invention is used as calcium ion-selective electrode and is used, and can measure whole blood, serum or other solution example, and adopts this sense film can in sample, select to measure calcium ion accurately.
Claims (3)
1. one kind is used for the calcium ion-selective electrode sense film, even with solute and one or more solvent, film forming after the solvent evaporates; Said solute is made up of the plastifier of 20-40% polymkeric substance, 50-75%, the ion selectivity carrier of 1-8% and the liposoluble salt of 0.5-3%, all calculates with the percentage by weight that accounts for the solute general assembly (TW), and the total content of solute is 150-400mg/ml;
Described polymkeric substance is the carboxyl PVC, high molecular weight pvc, or one or more of polyurethane;
Described plastifier is one or both of two-(2-ethylhexyl) last of the ten Heavenly stems two, ortho-nitrophenyl octyl ether;
Described ion selectivity carrier is a laurate;
Described liposoluble salt is potassium four (4-chlorphenyl) boric acid;
Described solvent is the potpourri of tetrahydrofuran or tetrahydrofuran, acetone.
2. sense film working method that is used for calcium ion-selective electrode; Stir as solute and solvent with the said polymkeric substance of claim 1, plastifier, ion selectivity carrier, liposoluble salt; Wherein the total content of solute is 150-400mg/ml; Mix at normal temperatures and stirred 12 hours, producing after volatilizing then 72 hours and making thickness is 0.5mm left and right sides sense film.
3. one kind is used for the application of the sense film of calcium ion-selective electrode like claim 1, is applied to measure the block sense film ion-selective electrode of electrolytic calcium ion test ion.
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| CN201210244731.1A CN102735736B (en) | 2012-07-16 | 2012-07-16 | Induction film used for calcium ion-selective electrode, production method and application thereof |
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| CN201210244731.1A CN102735736B (en) | 2012-07-16 | 2012-07-16 | Induction film used for calcium ion-selective electrode, production method and application thereof |
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| CN102735736B CN102735736B (en) | 2014-02-26 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104374814A (en) * | 2014-12-04 | 2015-02-25 | 上海惠中医疗科技有限公司 | Chloride-ion selective electrode sensing film and production method thereof |
| CN104849335A (en) * | 2015-05-29 | 2015-08-19 | 李宏奎 | Method for detecting ionic calcium content of blood sample |
| CN110734583A (en) * | 2019-09-05 | 2020-01-31 | 北京华科仪科技股份有限公司 | Induction film of calcium ion selective electrodes and preparation method thereof |
| CN114235928A (en) * | 2021-12-09 | 2022-03-25 | 铂恩医疗科技(深圳)有限公司 | Calcium ion selective electrode induction membrane solution, preparation method and application thereof |
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| EP1087225A2 (en) * | 1999-09-24 | 2001-03-28 | Ortho-Clinical Diagnostics, Inc. | Polymeric compositions for ion-selective electrodes |
| CN102507696A (en) * | 2011-10-19 | 2012-06-20 | 浙江大学 | Ion-selective electrode based on graphene electrode and preparation method thereof |
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2012
- 2012-07-16 CN CN201210244731.1A patent/CN102735736B/en not_active Expired - Fee Related
Patent Citations (2)
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|---|---|---|---|---|
| EP1087225A2 (en) * | 1999-09-24 | 2001-03-28 | Ortho-Clinical Diagnostics, Inc. | Polymeric compositions for ion-selective electrodes |
| CN102507696A (en) * | 2011-10-19 | 2012-06-20 | 浙江大学 | Ion-selective electrode based on graphene electrode and preparation method thereof |
Non-Patent Citations (6)
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| RITU KATAKY: "Comparative study of tripodal oxa-amides and oxa-esters as ionophores in potentiometric ion-selective electrodes for alkali and alkaline earth cations", 《ANALYTICA CHIMICA ACTA》, 31 December 1993 (1993-12-31) * |
| ULRICH SCHALLER 等: "Carrier Mechanism of Acidic Ionophores in Solvent Polymeric Membrane Ion-Selective Electrodes", 《ANALYTICAL CHEMISTRY》, 15 September 1995 (1995-09-15) * |
| ULRICH SCHALLER等: "Ionic Additives for Ion-Selective Electrodes Based on Electrically Charged Carriers", 《ANALYTICAL CHEMISTRY》, 1 February 1994 (1994-02-01) * |
| WERNER E等: "Design of a Calcium-Selective Optode Membrane Based on Neutral Ionophores", 《ANALYTICAL CHEMISTRY》, 1 April 1990 (1990-04-01) * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104374814A (en) * | 2014-12-04 | 2015-02-25 | 上海惠中医疗科技有限公司 | Chloride-ion selective electrode sensing film and production method thereof |
| CN104849335A (en) * | 2015-05-29 | 2015-08-19 | 李宏奎 | Method for detecting ionic calcium content of blood sample |
| CN110734583A (en) * | 2019-09-05 | 2020-01-31 | 北京华科仪科技股份有限公司 | Induction film of calcium ion selective electrodes and preparation method thereof |
| CN114235928A (en) * | 2021-12-09 | 2022-03-25 | 铂恩医疗科技(深圳)有限公司 | Calcium ion selective electrode induction membrane solution, preparation method and application thereof |
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| CN102735736B (en) | 2014-02-26 |
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Effective date of registration: 20180927 Address after: 200437 1-5 floor, 5 building, 1111 North Zhongshan Road, Hongkou District, Shanghai. Patentee after: Shanghai Zhongke run Da Medical Laboratory Laboratory Co., Ltd. Address before: 201206 3, 1 building, 1295 new Jin Qiao Road, Pudong New Area, Shanghai. Patentee before: Shanghai Huizhong Medical Technology Co., Ltd. |
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