CN104839426B - A kind of biological fermentation feed and preparation method thereof - Google Patents

A kind of biological fermentation feed and preparation method thereof Download PDF

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CN104839426B
CN104839426B CN201510206609.9A CN201510206609A CN104839426B CN 104839426 B CN104839426 B CN 104839426B CN 201510206609 A CN201510206609 A CN 201510206609A CN 104839426 B CN104839426 B CN 104839426B
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fermentation
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biofermentation
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albumen
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宋鹏
杨赛
孙立枢
任付平
李爱江
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Tianjin Naer Biotechnology Co Ltd
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Henan University of Science and Technology
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Abstract

The invention belongs to biological feedstuff field, and in particular to a kind of biological fermentation feed and preparation method thereof.Not only containing medicinal herb componentses such as rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis and honeysuckles in the live pig biological fermentation feed of the present invention, it is possible to increase the ability of live pig resisting stress and the resistance to disease, it is horizontal to reduce live pig Blood Cholesterol.Simultaneously also containing the utilizable biofermentation albumen of live pig, fat, mineral matter, vitamin, a variety of high energy capacity materials, it is nutritious, and a large amount of active and inactive beneficial microbes, these materials can provide prebiotic former class material to gastrointestinal microbiological, improve the living environment of gastrointestinal microbiological, adjust the growth of microorganism and breed, whole gastrointestinal microbiological is in a kind of normal fast-developing situation, promote to wriggle, reduce gastrointestinal disease.It can effectively facilitate live pig weightening while reducing feeding fine fodder, reducing cost, improve meat quality, make pork fresh and tender tasty.

Description

A kind of biological fermentation feed and preparation method thereof
Technical field
The invention belongs to biological feedstuff field, and in particular to a kind of biological fermentation feed and preparation method thereof.
Background technology
As the improvement of people's living standards, for the demand more and more higher of pollution-free food, pollution-free food, pork and its The internal organs meat traditional as Chinese people is even more to receive much concern.At present, the common feedstuffses species of pig is a lot, is divided into by nutrition Protein feeds, energy feed, roughage, green forage, ensilage, mineral feed and the major class of feed addictive 7.Although All kinds of feeds have respective distinctive effect, and the feed collocation of the pig of various different cultivars is also required, particularly vegetalitas, ANFs in animality and mineral material is unfavorable for digesting and assimilating for pig.Meanwhile in the production of pork pig, because of anxious work( Nearly profit causes excessive using antibiotic and abuse prohibited items, and the harmful substance residual in animal food may be exceeded, sternly Ghost image rings the product quality of pork, so as to influence the health of people.Therefore, the different approaches for pork product, mode And degree, environment-friendly feed substitute is studied, the feed or additive for substituting those to easily cause pork pollution, so as to produce The safety-type pork product of high quality a, it has also become new trend of future in feed industry.
Biological fermentation feed refers to that under manual control condition microorganism by vegetalitas, is moved by the metabolic activity of itself ANFs in physical property and mineral material decomposes or conversion, and generation can more be searched for food, digest by livestock and poultry, absorbing and nonhazardous The feedstuff of effect.
At present, biological fermentation feed has that strain source is complicated, trophic level is low, limited to the enteron aisle improvement result of pig etc. Defect, these are not only related to fermented bacterium and zymotechnique, and fermentation substrate also has a certain impact to it.
The content of the invention
It is an object of the invention to provide a kind of biological fermentation feed and preparation method thereof.Immunity can be effectively improved, is promoted Enter appetite, improve food-intake, smoothly excretion, anti-bacteria and anti-virus, reduces the generation of disease.
The present invention is adopted the technical scheme that a kind of biological fermentation feed, the feed is by giving birth to solve the above problems Thing fermentation protein, rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle are made, and prepare the weight of each component needed for feed It is respectively -12 parts of biofermentation protein 10, rheum officinale 1-1.5 parts, radix scutellariae 2-4 parts, adenophora tetraphylla 1-2 parts, santal 1-1.5 to measure number Part, Radix Isatidis 1-2 parts, honeysuckle 2-3 parts;
The biofermentation albumen is using wheat bran, dregs of beans, sweet potato waste, pomace as fermentation substrate, using urea and sodium chloride to add Add agent, formed by fermented bacterium fermentation of geotrichum candidum, bacillus megaterium and trichoderma reesei.
The preparation method of the biological fermentation feed, comprises the following steps:
Step 1: crush
Rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, use powder Broken machine crosses 40 mesh sieves after crushing, standby;
Step 2: fermentation substrate pre-processes
It is 5 by weight by wheat bran, dregs of beans, sweet potato waste and pomace:1:2:2 are mixed and stirred for uniformly rear addition urea and chlorine Change sodium, fermentate is made, and wheat bran, dregs of beans, sweet potato waste, pomace, the weight ratio of urea and sodium chloride are 100:20:40:40:4: 1, then by fermentate and water be 20 by volume:3 are mixed and stirred for uniformly, that is, fermentation substrate being made, standby;
Step 3: inoculation strain
Trichoderma reesei is first added into fermentation substrate to stir, and after 30 DEG C ferment 36 hours, adds huge gemma bar Bacterium and geotrichum candidum, the inoculation total amount of strain is 3%, and each strain number ratio is geotrichum candidum:Bacillus megaterium:Trichoderma reesei= 3:2:1;
Step 4: fermentation
After step 3 is inoculated with into 30 DEG C of fermentation 36h of fermentation substrate of strain, it is 80- to take out tunning and be put into temperature 90 DEG C of drying indoor seasoning, until the water content of tunning is less than 10%, biofermentation albumen is made, it is standby;
Step 5: the preparation of biological fermentation feed
By the parts by weight of each component weigh biofermentation albumen, rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis and Honeysuckle, biological fermentation feed is made after stirring.
Geotrichum candidum in the present invention is preserved in China General Microbiological culture presevation administrative center, and preservation bacterium number is CGMCC 2.1132.Bacillus megaterium is preserved in China General Microbiological culture presevation administrative center, and preservation bacterium number is CGMCC 1.2416.Trichoderma reesei is preserved in China General Microbiological culture presevation administrative center, and preservation bacterium number is CGMCC 3.5218.With Upper strain is purchased from Shandong abyssopelagic organism Science and Technology Co., Ltd..
In the present invention the effect of each component:
Sorrel rhubarb stem is red, gas delicate fragrance, bitter and micro-puckery, and that chews sticks to one's teeth, and has sand feeling.With attacking stagnant, clearing away damp-heat, rush down Fire, cool blood, dissolving stasis, removing toxic substances and other effects.Radix scutellariae is lamiaceae labiatae scutellaria herbaceos perennial, and root is used as medicine, bitter, cold in nature, is had Heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, antiabortive and other effects.Cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, neonatal jaundice caused by dampness-heat, The diseases such as pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swells furuncle sore.The clinical antibiotic property of radix scutellariae is better than the coptis, and And do not develop immunity to drugs.Adenophora tetraphylla is Campanulaceae herbaceos perennial, first recorded in the Eastern Han Dynasty《Sheng Nong's herbal classic》, top grade is classified as, And describe and have that " main hematocele shies gas, except fever and chills, bowl spares benefit lung qi." function.Santal, it is the drying of sandalwood plants santal trunk Heartwood.Cure mainly and be used for cold obstruction causing qi stagnation, chest diaphragm does not relaxes, chest impediment and cardialgia, epigastric pain, vomit deficiency of food.Radix Isatidis is planted for Cruciferae The dry root of thing woaded blue, is generally excavated in the fall, can be used as medicine after processing.Its is cold in nature, and taste is first bitter after micro- sweet tea, has heat-clearing The effect of removing toxic substances, preventing cold, relieving sore-throat.It is mainly used in treating the diseases such as the dark reddish purple dark, scarlet fever of febrile virulent maculae, tongue.Honeysuckle For Caprifoliaceae woodbine honeysuckle and congener dry flower or with the flower just opened.The sweet cold air fragrance of its property, clear heat with drugs of sweet flavour and cold nature Without injuring one's stomach, fragrance reaches thoroughly again can eliminating evil.Honeysuckle can dispelling wind-heat, also kind removing summer-heat blood poison, for various febrile diseases, such as body The diseases such as heat, dermexanthesis, hair spot, heat toxin sore carbuncle, abscess of throat, equal significant effect.
The main component of Chinese herbal medicine has alkaloid, polysaccharide, protein, organic acid, glucosides, flavone compound and terpene Compound etc., mostly has nutrition and medical value concurrently, and it is increase immunity of organisms that it, which is acted on, improves organismic internal environment, is improved disease-resistant Power, anti-bacteria and anti-virus, improve a poor appetite, strengthen anti-stress effect, improve livestock and poultry production performance and product quality.Add Chinese herbal medicine energy Enough solve to endanger the antibiotic residue and problem of environmental pollution of people's health during animal husbandry development, while shorten raising Time, feeding cost is reduced, improve production efficiency, there is important economic implications and social benefit.
Beneficial effect
Not only contain rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis and gold and silver in the live pig biological fermentation feed of the present invention The medicinal herb componentses such as flower, it is possible to increase the ability of live pig resisting stress and the resistance to disease, reduce live pig Blood Cholesterol It is horizontal.Also contain the utilizable biofermentation albumen of live pig, fat, mineral matter, vitamin, a variety of high energy capacity materials simultaneously, It is nutritious, and a large amount of active and inactive beneficial microbe, these materials can give gastrointestinal microbiological to provide prebiotic former class Material, improve the living environment of gastrointestinal microbiological, adjust the growth of microorganism and breed, whole gastrointestinal microbiological is in one kind Normal fast-developing situation, promote to wriggle, reduce gastrointestinal disease.Can have while reducing feeding fine fodder, reducing cost Effect promotes live pig weightening, improves meat quality, makes pork fresh and tender tasty.
Embodiment
A kind of biological fermentation feed, the feed is by biofermentation albumen, rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis It is made with honeysuckle, and the parts by weight for preparing each component needed for feed are respectively -12 parts of biofermentation protein 10, rheum officinale 1- 1.5 parts, radix scutellariae 2-4 parts, adenophora tetraphylla 1-2 parts, santal 1-1.5 parts, Radix Isatidis 1-2 parts, honeysuckle 2-3 parts;
The biofermentation albumen is using wheat bran, dregs of beans, sweet potato waste, pomace as fermentation substrate, using urea and sodium chloride to add Add agent, formed by fermented bacterium fermentation of geotrichum candidum, bacillus megaterium and trichoderma reesei.
The preparation method of the biological fermentation feed, comprises the following steps:
Step 1: crush
Rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, use powder Broken machine crosses 40 mesh sieves after crushing, standby;
Step 2: fermentation substrate pre-processes
It is 5 by weight by wheat bran, dregs of beans, sweet potato waste and pomace:1:2:2 are mixed and stirred for uniformly rear addition urea and chlorine Change sodium, fermentate is made, and wheat bran, dregs of beans, sweet potato waste, pomace, the weight ratio of urea and sodium chloride are 100:20:40:40:4: 1, then by fermentate and water be 20 by volume:3 are mixed and stirred for uniformly, that is, fermentation substrate being made, standby;
Step 3: inoculation strain
Trichoderma reesei is first added into fermentation substrate to stir, and after 30 DEG C ferment 36 hours, adds huge gemma bar Bacterium and geotrichum candidum, the inoculation total amount of strain is 3%, and each strain number ratio is geotrichum candidum:Bacillus megaterium:Trichoderma reesei= 3:2:1;
Step 4: fermentation
After step 3 is inoculated with into 30 DEG C of fermentation 36h of fermentation substrate of strain, it is 80- to take out tunning and be put into temperature 90 DEG C of drying indoor seasoning, until the water content of tunning is less than 10%, biofermentation albumen is made, it is standby;
Step 5: the preparation of biological fermentation feed
By the parts by weight of each component weigh biofermentation albumen, rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis and Honeysuckle, biological fermentation feed is made after stirring.
Embodiment 1
A kind of biological fermentation feed, the feed is by biofermentation albumen, rheum officinale, radix scutellariae, adenophora tetraphylla, santal and honeysuckle It is made, and the parts by weight for preparing each component needed for feed are respectively biofermentation protein 10 part, 1 part of rheum officinale, 2 parts of radix scutellariae, south 1 part of the root of straight ladybell, 1 part of santal, 1 part of Radix Isatidis, 2 parts of honeysuckle;
The biofermentation albumen is using wheat bran, dregs of beans, sweet potato waste, pomace as fermentation substrate, using urea and sodium chloride to add Add agent, formed by fermented bacterium fermentation of geotrichum candidum, bacillus megaterium and trichoderma reesei.
The preparation method of the biological fermentation feed, comprises the following steps:
Step 1: crush
Rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, use powder Broken machine crosses 40 mesh sieves after crushing, standby;
Step 2: fermentation substrate pre-processes
It is 5 by weight by wheat bran, dregs of beans, sweet potato waste and pomace:1:2:2 are mixed and stirred for uniformly rear addition urea and chlorine Change sodium, fermentate is made, and wheat bran, dregs of beans, sweet potato waste, pomace, the weight ratio of urea and sodium chloride are 100:20:40:40:4: 1, then by fermentate and water be 20 by volume:3 are mixed and stirred for uniformly, that is, fermentation substrate being made, standby;
Step 3: inoculation strain
Trichoderma reesei is first added into fermentation substrate to stir, and after 30 DEG C ferment 36 hours, adds huge gemma bar Bacterium and geotrichum candidum, the inoculation total amount of strain is 3%, and each strain number ratio is geotrichum candidum:Bacillus megaterium:Trichoderma reesei= 3:2:1;
Step 4: fermentation
After step 3 is inoculated with into 30 DEG C of fermentation 36h of fermentation substrate of strain, it is 80- to take out tunning and be put into temperature 90 DEG C of drying indoor seasoning, until the water content of tunning is less than 10%, biofermentation albumen is made, it is standby;
Step 5: the preparation of biological fermentation feed
By the parts by weight of each component weigh biofermentation albumen, rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis and Honeysuckle, biological fermentation feed is made after stirring.
Embodiment 2
A kind of biological fermentation feed, the feed is by biofermentation albumen, rheum officinale, radix scutellariae, adenophora tetraphylla, santal and honeysuckle Be made, and the parts by weight for preparing each component needed for feed be respectively biofermentation protein 12 part, 1.5 parts of rheum officinale, 4 parts of radix scutellariae, 2 parts of adenophora tetraphylla, 1.5 parts of santal, 2 parts of Radix Isatidis, 3 parts of honeysuckle;
The biofermentation albumen is using wheat bran, dregs of beans, sweet potato waste, pomace as fermentation substrate, using urea and sodium chloride to add Add agent, formed by fermented bacterium fermentation of geotrichum candidum, bacillus megaterium and trichoderma reesei.
The preparation method of the biological fermentation feed, comprises the following steps:
Step 1: crush
Rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace are dried, use powder Broken machine crosses 40 mesh sieves after crushing, standby;
Step 2: fermentation substrate pre-processes
It is 5 by weight by wheat bran, dregs of beans, sweet potato waste and pomace:1:2:2 are mixed and stirred for uniformly rear addition urea and chlorine Change sodium, fermentate is made, and wheat bran, dregs of beans, sweet potato waste, pomace, the weight ratio of urea and sodium chloride are 100:20:40:40:4: 1, then by fermentate and water be 20 by volume:3 are mixed and stirred for uniformly, that is, fermentation substrate being made, standby;
Step 3: inoculation strain
Trichoderma reesei is first added into fermentation substrate to stir, and after 30 DEG C ferment 36 hours, adds huge gemma bar Bacterium and geotrichum candidum, the inoculation total amount of strain is 3%, and each strain number ratio is geotrichum candidum:Bacillus megaterium:Trichoderma reesei= 3:2:1;
Step 4: fermentation
After step 3 is inoculated with into 30 DEG C of fermentation 36h of fermentation substrate of strain, it is 80- to take out tunning and be put into temperature 90 DEG C of drying indoor seasoning, until the water content of tunning is less than 10%, biofermentation albumen is made, it is standby;
Step 5: the preparation of biological fermentation feed
By the parts by weight of each component weigh biofermentation albumen, rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis and Honeysuckle, biological fermentation feed is made after stirring.
Test influence of 1 biological fermentation feed to grower pigs production performance
Experiment selection parity, age in days and the substantially similar pig 45 of body weight, are randomly divided into tri- groups, every group 15 of I, II and III Head, male and female half and half.I groups compare, and do not add any biological fermentation feed, only feed basic mixed fodder, II groups are on the basis of I groups Only addition biofermentation albumen, III groups add biological fermentation feed on the basis of I groups.Test pig uses group feeding, feeds 2 times day, from By drinking water, other management are carried out according to a conventional method.5 days are preliminary trial period before experiment, and feed management method is identical.As shown in table 1, it is raw After long pig feeding biofermentation albumen and biological fermentation feed, weightening all significantly improves than control group I, while reduces feed-weight ratio, Improve feed efficiency, save feed cost, increase economic efficiency.
The weightening of the test pig of table 1, feed intake and feed conversion ratio
Test influence of 2 biological fermentation feeds to quality of pig trunk and internal organ character
Influence of the biological fermentation feed of table 2 to quality of pig trunk and internal organ character
As shown in table 2, Slaughter tests showed shows, the index and conscience etc. such as each group hog slaughtering rate, fat thickness, lean meat percentage Visceral weight is all improved, and slaughter experiment has no that internal organs observe lesion, shows that biological fermentation feed is raised as live pig Material, also has health care growth promoting function to quality of pig trunk and animal tissue's organ.

Claims (2)

1. a kind of live pig biological fermentation feed, it is characterised in that be made up of the component of following parts by weight:Biofermentation albumen 10-12 parts, rheum officinale 1-1.5 parts, radix scutellariae 2-4 parts, adenophora tetraphylla 1-2 parts, santal 1-1.5 parts, Radix Isatidis 1-2 parts, honeysuckle 2-3 Part;
The biofermentation albumen is using wheat bran, dregs of beans, sweet potato waste, pomace as fermentation substrate, using urea and sodium chloride as additive, Formed by fermented bacterium fermentation of geotrichum candidum, bacillus megaterium and trichoderma reesei;Wherein fermentation substrate pretreatment, inoculation strain And fermentation step is as follows successively:
1)Fermentation substrate pre-processes:It is 5 by weight by wheat bran, dregs of beans, sweet potato waste and pomace:1:2:2 are mixed and stirred for uniformly Urea and sodium chloride are added afterwards, is made fermentate, and wheat bran, dregs of beans, sweet potato waste, pomace, the weight ratio of urea and sodium chloride are 100:20:40:40:4:1, then by fermentate and water be 20 by volume:3 are mixed and stirred for uniformly, that is, fermentation substrate being made, standby With;
2)It is inoculated with strain:Trichoderma reesei is first added into fermentation substrate to stir, and after 30 DEG C ferment 36 hours, is added huge Bacillus and geotrichum candidum, the inoculation total amount of strain is 3%, and each strain number ratio is geotrichum candidum:Bacillus megaterium:Richter scale Trichoderma=3:2:1;
3)Fermentation:After step 3 is inoculated with into 30 DEG C of the fermentation substrate fermentation 36h of strain, takes out tunning and be put into temperature and be 80-90 DEG C of drying indoor seasoning, until the water content of tunning is less than 10%, biofermentation albumen is made.
2. the preparation method of a kind of live pig biological fermentation feed according to claim 1, it is characterised in that including following Step:
Step 1: crush:By rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis, honeysuckle, wheat bran, dregs of beans, sweet potato waste and pomace Dry, 40 mesh sieves are crossed after being crushed with pulverizer, it is standby;
Step 2: carry out fermentation substrate pretreatment by step described in claim 1;
Step 3: carry out inoculation strain by step described in claim 1;
Step 4: being fermented by step described in claim 1, biofermentation albumen is made, it is standby;
Step 5: the preparation of biological fermentation feed:The weight ratio of each component as described in claim 1 weigh biofermentation albumen, Rheum officinale, radix scutellariae, adenophora tetraphylla, santal, Radix Isatidis and honeysuckle, biological fermentation feed is made after stirring.
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CN105994977A (en) 2016-01-28 2016-10-12 深圳市七彩地农业科技有限公司 Non-grain ecological feed, preparation method and feeding method thereof
CN107410687A (en) * 2017-05-18 2017-12-01 佛山市高明区生产力促进中心 A kind of feed for being favorably improved live pig degree Fresh & Tender in Texture
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CN109938189A (en) * 2019-04-25 2019-06-28 湖南加丰生物科技有限公司 A kind of live pig rheum officinale no-antibiotics feed and preparation method thereof

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