CN104833747B - A kind of preparative hplc UV-detector using deep ultraviolet LED light source - Google Patents
A kind of preparative hplc UV-detector using deep ultraviolet LED light source Download PDFInfo
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- CN104833747B CN104833747B CN201510240792.4A CN201510240792A CN104833747B CN 104833747 B CN104833747 B CN 104833747B CN 201510240792 A CN201510240792 A CN 201510240792A CN 104833747 B CN104833747 B CN 104833747B
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- led
- glass tubing
- detector
- light source
- deep ultraviolet
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Abstract
The present invention relates to a kind of preparative hplc UV-detector using deep ultraviolet LED light source, it comprises shell, base, glass tubing, photomask, power supply, condenser lens, light cell, light cell adjusting bracket, control circuit plate, LED, signal output part；Glass tubing is fixed on the middle part of base by support；LED is respectively placed in the both sides of glass tubing and aligns with the axis of glass tubing with light cell；Condenser lens is placed in the rear portion of LED, the head alignment condenser lens of LED.The present invention uses wide bandwidth deep ultraviolet LED etc. for light source, without light-splitting processing, using the glass tubing as a stream part directly as detection cell, simplifies structure；Condenser lens, to from whole light focusings of LED through detection cell, obtains the luminous flux of maximum, improves detection sensitivity.
Description
[technical field]
The present invention relates to liquid chromatographic detection technical field, specifically, be a kind of employing deep ultraviolet LED
The preparative hplc UV-detector of light source.
[background technology]
UV-detector is the most frequently used detection of preparative liquid chromatography, monitoring means.Common ultraviolet is examined
Survey device is with mercury lamp as light source, by the means such as light splitting, focusing, makes the ultraviolet light of target wavelength by detection
Pond, and measure its uv absorption intensity, calculate its concentration by Lang Bote-Beer law further.
Chromatograph UV-detector with LED as light source obtains bigger development in recent years, and this has benefited from LED
The fast development of technology, new technique can be greatly reduced deep UV LED cost, and Japan has begun to scale
Metaplasia produce emission wavelength be 255～350nm deep ultraviolet LED, deep UV LED to a certain extent may be used
To be used for substituting finsen lamp, the application of LED is made to be widened.
The feature of LED light source is to have narrow spectral bandwidth, and selection is mated with sample maximum absorption wavelength
LED is as ultraviolet source, it might even be possible to does not use optical filtering to process and can be obtained by higher detection sensitivity.
But, on the one hand, select Wavelength matched LED light source more difficult, on the other hand, the valency of deep ultraviolet LED
Lattice are general costly, and the life-span is relatively low, and the deep ultraviolet LED price of narrow spectral half width is higher.
Preparative hplc is to prepare the best means of high purity product, the field such as prepares at medicine, high purity reagent
It has been used widely.Complicated material sample, after chromatographic column separates, can obtain the list that purity is the highest
One component, additionally, the concentration that the target product of preparative hplc is in effluent is the highest and the most multiple
Miscellaneous.Common scale preparing chromatography system is all equipped with shunting ultraviolet detection system, to whole preparative separation
Process is monitored.And for general mesolow preparation system, the method mostly using off-line, collect
HPLC is used to carry out separating analysis after sample.
Preparative liquid chromatography has that sample concentration is higher, disturb less feature, therefore detects it
The requirement of sensitivity of UV-detector the highest.The present invention, with wide spectrum LED as light source, uses
The pattern of complete wavelength range absorption detecting, designs novel low-cost, Simple UV-detector, effectively drops
The installation cost of low preparing chromatography system, can meet and monitor preparative hplc separation process in real time,
Ensure the requirement of product quality.
[summary of the invention]
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of employing deep ultraviolet LED light source
Preparative hplc UV-detector.
It is an object of the invention to be achieved through the following technical solutions:
A kind of preparative hplc UV-detector using deep ultraviolet LED light source, it comprises shell, base,
Glass tubing, photomask, power supply, condenser lens, light cell, light cell adjusting bracket, control circuit plate, LED
Lamp, signal output part；It is characterized in that, glass tubing is fixed on the middle part of base by support；LED
Lamp is respectively placed in the both sides of glass tubing and aligns with the axis of glass tubing with light cell；Condenser lens is placed in
The rear portion of LED, the head alignment condenser lens of LED；Right opposite in LED arranges light cell and adjusts
Joint frame.
UV-detector is used photomask by body seal, the exposed portion as the glass tubing of detection cell
Seal.
Described base and outlet, signal output part are fixed in the shell of sealing.
Described shell can be metal material or plastic material.
The length of described glass tubing is more than 2 times of base, and internal diameter can adjust as required, 100
Micron-5 centimetres, can be respectively used to shunting detection and directly detection.
Battery provides power supply for LED and panel.The signal exported by light cell is introduced into control circuit
Plate, exports computer disposal from UPS port after amplifying.
By bracket adjustment condenser lens, the light sent by LED is made all to be focused on glass tubing as far as possible,
And ensure that the hot spot on glass tubing is close with bore.
Owing in the material of glass tubing, thickness, pipe, the character of solution all can produce different refractive indexs, can
Up and down, front and back to be regulated light cell by photronic support so that it is be just placed in and appeared by glass tubing
Light focus on.
Take into full account glass tubing and the refractive index of liquid wherein flowed, for making light cell be placed in Jiao of light
Near Dian, the right opposite in LED arranges light cell adjusting bracket, can raise in up and down and back and forth direction
Whole, to ensure to absorb more light.
Base and outlet, signal output socket are fixed in the shell of sealing, and shell can be gold
Belong to material, it is also possible to be plastic material, but to ensure its opacity.
Glass tubing passes from the two of shell sections, and it is exposed to outer part and is also required to use shading treatment, keeps away
Exempt from veiling glare to enter, impact detection.
Compared with prior art, the positive effect of the present invention is:
Employing wide bandwidth deep ultraviolet LED etc. are light source, without light-splitting processing, using as a stream part
Glass tubing directly as detection cell, simplify structure.Whole light focusings from LED are worn by condenser lens
Cross detection cell, obtain the luminous flux of maximum, improve detection sensitivity.
[accompanying drawing explanation]
Fig. 1 structure drawing of device；
The focusing of Fig. 2 light and detection；
Labelling in accompanying drawing: 1. shell, 2. glass tubing；3. photomask；4. power supply；5. condenser lens；
6. light cell；7. light cell adjusting bracket；8. control circuit plate；9.LED lamp；10. signal output part.
[detailed description of the invention]
The tool of a kind of preparative hplc UV-detector using deep ultraviolet LED light source of the present invention presented below
Body embodiment.
Embodiment 1
Refer to accompanying drawing 1,2, a kind of preparative hplc UV-detector using deep ultraviolet LED light source,
It comprises shell, base, glass tubing, photomask, power supply, condenser lens, light cell, and light cell regulates
Frame, control circuit plate, LED, signal output part；It is characterized in that, glass tubing is consolidated by support
It is scheduled on the middle part of base；LED and light cell be respectively placed in glass tubing both sides and with the axis of glass tubing
Line aligns；Condenser lens is placed in the rear portion of LED, the head alignment condenser lens of LED；In LED
Right opposite light cell adjusting bracket is set.
A kind of preparative hplc UV-detector using deep ultraviolet LED light source, whole system is by shell 1
Seal, be also used photomask 3 as the exposed portion of the glass tubing 2 of detection cell and seal, it is to avoid environment
The entrance of veiling glare, reduces background absorption, improves detection sensitivity.
Glass tubing 2 is the quartz glass tube of a pass-through facility, LED 9 light sent, through optically focused
After mirror 5 optically focused, being reflected on detection cell 2, after sample solution, the focus of light falls at photoelectricity
On pond 6.
Take into full account glass tubing and the refractive index of liquid wherein flowed, for making light cell 6 be placed in light
Near focal point, arranges light cell adjusting bracket 7, can adjust in up and down and back and forth direction, permissible to ensure
Absorb more light.
The power supply of LED 9 is lithium battery 4, light cell 6 signal of telecommunication obtained is admitted to control electricity
Road plate 8, and export and carry out on computer 9 showing and processing.
Detection cell glass tubing 2 can regulate caliber and length as required；Can directly and column outlet
It is connected, or detects after the effluent shunting of chromatographic column.
Base and outlet, signal output part 10 are fixed in the shell of sealing, and shell can be gold
Belong to material, it is also possible to be plastic material, but to ensure its opacity.
Can be split or not shunt direct and as detection cell glass by preparative liquid chromatography post effluent
Pipe is connected, and switches on power.Identical with the operation principle of general detector.Light is through containing sample
Solution.Owing to different samples are different to the absorbability of different wave length ultraviolet light, light cell is caused to be collected
Signal intensity produce change.Whether the change judgement sample according to signal flows out, purity and concentration etc.
Parameter.
The above is only the preferred embodiment of the present invention, it is noted that general for the art
Logical technical staff, without departing from the inventive concept of the premise, it is also possible to make some improvements and modifications,
These improvements and modifications also should be regarded as in protection scope of the present invention.
Claims (6)
1. using a preparative hplc UV-detector for deep ultraviolet LED light source, it comprises shell, base, glass tubing, photomask, power supply, condenser lens, light cell, light cell adjusting bracket, control circuit plate, LED, signal output part；It is characterized in that, glass tubing is fixed on the middle part of base by support；LED and light cell are respectively placed in the both sides of glass tubing,And LED Lamp aligns with the axis of glass tubing with photronic line；Condenser lens is placed in the rear portion of LED, the head alignment condenser lens of LED；Right opposite in LED arranges light cell adjusting bracket.
A kind of preparative hplc UV-detector using deep ultraviolet LED light source the most as claimed in claim 1, it is characterised in that UV-detector is used photomask sealing by body seal, the exposed portion as the glass tubing of detection cell.
A kind of preparative hplc UV-detector using deep ultraviolet LED light source the most as claimed in claim 1, it is characterised in that described base and outlet, signal output part are fixed in the shell of sealing.
A kind of preparative hplc UV-detector using deep ultraviolet LED light source the most as claimed in claim 1, it is characterised in that described shell is metal material or plastic material.
A kind of preparative hplc UV-detector using deep ultraviolet LED light source the most as claimed in claim 1, it is characterised in that the length of described glass tubing is more than 2 times of base.
A kind of preparative hplc UV-detector using deep ultraviolet LED light source the most as claimed in claim 1, it is characterized in that, battery provides power supply for LED and panel, light cell the signal exported is introduced into control circuit plate, exports computer disposal from UPS port after amplifying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510240792.4A CN104833747B (en) | 2015-05-06 | 2015-05-06 | A kind of preparative hplc UV-detector using deep ultraviolet LED light source |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510240792.4A CN104833747B (en) | 2015-05-06 | 2015-05-06 | A kind of preparative hplc UV-detector using deep ultraviolet LED light source |
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| Publication Number | Publication Date |
|---|---|
| CN104833747A CN104833747A (en) | 2015-08-12 |
| CN104833747B true CN104833747B (en) | 2016-08-24 |
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| CN201510240792.4A Expired - Fee Related CN104833747B (en) | 2015-05-06 | 2015-05-06 | A kind of preparative hplc UV-detector using deep ultraviolet LED light source |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108461613A (en) * | 2018-05-31 | 2018-08-28 | 深圳市瑞丰光电紫光技术有限公司 | A kind of UV-LED light sources and its lamps and lanterns |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1050298A (en) * | 1974-05-15 | 1979-03-13 | Kenneth E. Nelson | Photometric system with conical flow cell |
| JPS6373137A (en) * | 1986-09-17 | 1988-04-02 | Showa Denko Kk | Chemical light-emission detector for liquid chromatography |
| CA2306791C (en) * | 1997-10-24 | 2007-05-15 | Northeastern University | A multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis |
| CN1287140C (en) * | 2000-09-25 | 2006-11-29 | 松下电器产业株式会社 | Device for chromatographic quantitative measurement |
| US7268868B2 (en) * | 2004-10-29 | 2007-09-11 | Palo Alto Research Center Incorporated | Anti-resonant waveguide sensors |
| CN1924556A (en) * | 2005-08-29 | 2007-03-07 | 深圳雷杜生命科学股份有限公司 | Optical path system for clinical test analyzer |
| CN100371710C (en) * | 2006-02-20 | 2008-02-27 | 厦门大学 | Portable liquid-phase chromatograph |
| US20110312751A1 (en) * | 2010-06-17 | 2011-12-22 | Geneasys Pty Ltd | Microfluidic device for detection of mitochondrial dna via fluorescence modulated by hybridization |
| CN102175805A (en) * | 2010-12-31 | 2011-09-07 | 大连依利特分析仪器有限公司 | Fixed wavelength detector for liquid chromatograph |
| US20120195806A1 (en) * | 2011-02-01 | 2012-08-02 | Tim Mulvihill | Solar Floater |
| CN202133646U (en) * | 2011-05-05 | 2012-02-01 | 北京清博华科技有限公司 | Fixed wavelength detector |
| CN203216912U (en) * | 2013-04-25 | 2013-09-25 | 上海伍丰科学仪器有限公司 | Monochromatic light generator of ultrahigh performance liquid chromatograph ultraviolet detector |
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