CN101747407A - High-efficient integrated system for separation and purification - Google Patents
High-efficient integrated system for separation and purification Download PDFInfo
- Publication number
- CN101747407A CN101747407A CN200810162337A CN200810162337A CN101747407A CN 101747407 A CN101747407 A CN 101747407A CN 200810162337 A CN200810162337 A CN 200810162337A CN 200810162337 A CN200810162337 A CN 200810162337A CN 101747407 A CN101747407 A CN 101747407A
- Authority
- CN
- China
- Prior art keywords
- separation
- sample
- hyperchannel
- purification
- pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The invention provides a high-efficient integrated system for separation and purification, comprising a fluid dispensing system, a sample introduction system, a separation system, a cleaning system, a communication system, a detector, a collection system and a corresponding control system, wherein the separation system is a chromatographic column; the fluid dispensing system, the sample introduction system and the cleaning system are connected with the communication system; the communication system is connected with the separation system; and the separation system is connected with the detector and the collection system. The invention mainly solves the technical problems of insufficient automation degree, incapability of massive and continuous production and the like, and provides a liquid-phase separation system which has rapid separation speed, multi-path on-line detection, unattended operation within 24h and capability of batch production.
Description
Technical field
The present invention relates to a peptide species high-efficient integrated system for separation and purification.
Background technology
The synthetic polypeptide is the low thick product of a kind of purity, must just can obtain high purity product through purifying, and all the time, the separation and purification problem is the biggest obstacle in the chemically synthesized polypeptide.The separation method commonly used of chemically synthesized polypeptide has three kinds: gel filtration chromatography, ion-exchange chromatography, RPLC.RPLC is the hydrophilic and hydrophobic difference according to polypeptide and analogue thereof, and they are separated.But it is difficult for mass-producing, and separation costs is higher.
Liquid phase chromatography is an important branch in the chromatography, existing liquid chromatography is mainly analysis mode, half preparation type and several classes of preparation type, analysis mode is used for the detection of sample, its objective is exactly to the accurately qualitative or quantitative analysis of micro-example sample introduction, and not obtaining product separation, half preparation type is to utilize the analysis mode instrument, only uses slightly bigger separator column instead and analyzes and obtain micro-product, the preparation type then further increases separator column, to obtain a small amount of product separation.But because pumping capacity is little, column volume is little etc. a series of problems, and in process of production, mostly be manually or semiautomatic controls, level of automation is not high, is difficult to scale operation.
Application number is 200410066339.8 highly effective liquid phase chromatographic system, is made up of transfusion system, sampling system, separation system, UV-detector, light path system, microcomputer control system, data handling system etc.High pressure constant flow pump is depressed into certain high pressure mutually with liquid-flow, and with the constant rate of setting flow through sampling system and separator column, thereby reaches the separation of analyte.UV-detector adopts deuterium lamp to make light source, the monochromatic ray of holographic concave grating diffraction is mapped to the half transmitting reflective mirror, be divided into the two-beam line, a branch of through shining behind the flow-through cell on the measurement photoelectric cells, another bundle shines on the reference photocell through reference cell, and photocell converts luminous energy to the Weak current signal.The sample component pond that circulates after chromatographic column is separated has optionally the monochromatic ray of specific wavelength and to absorb, and the light intensity that photosensor is measured has reduced, and the absorption of electromagnetic energy is realized the mensuration of sample concentration according to molecule.
The patent No. is a kind of biochemical Process for preparing liquid-phase chromatograph of 00133303.8, comprise the moving phase basin, inject the chromatographic working station etc. of pump, valve, chromatographic column, detector, collector and the control data collection and the processing of moving phase, wherein, described pump is normal pressure chromatogram peristaltic pump and middle pressure chromatogram titanium pump, described chromatographic column is the titanium alloy chromatographic column, described collector is an automatic fraction collector, and is equipped with ten channel valve as collecting by the peak, and described chromatographic working station is realized the control to pump, valve.This equipment can be advantageously used in the purifying of Bio-engineering Products, integrates normal pressure and middle pressure chromatographic separation, and volume is less, preparation cost is lower.
Above liquid chromatographic system all can not be realized full-automation, and can't satisfy large batch of separation.
Summary of the invention
The present invention solves the not enough and technical problem of continuous production etc. in enormous quantities of existing in prior technology level of automation, provide a kind of velocity of separation very fast, the online detection of hyperchannel, the liquid-phase separating system that unmanned operation automatically in 24 hours can be produced in enormous quantities.
Above-mentioned technical problem of the present invention is mainly solved by following technical proposals:
A kind of high-efficient integrated system of separation and purification, comprise liquid mixing system, sampling system, separation system, cleaning system, connected system, detector, gathering system and control corresponding system, separation system is a chromatographic column, liquid mixing system, sampling system and cleaning system are to connected system, connected system is connected to separation system, and separation system is connected to detector and gathering system.
Liquid mixing system comprises some kinds of eluents, correspondingly is provided with pump on the stream of each eluent, and pump is connected to mixing tank jointly, and mixing tank is connected to connected system.Eluent is mixed with elutriant according to certain mixed, and the flow of eluent and flow velocity are to be controlled by pump.
Sampling system comprises two sample intake passages, the sample reagent bottle that the passage connection capacity is bigger, a passage connects automatic sampler, automatic sampler can separately store the less sample of multiple capacity, two sample intake passages are connected to the hyperchannel valve jointly, the hyperchannel valve is connected to pump, and pump is connected to connected system.Automatic sampler can select to store the sample interior, with the sample output of needed kind, and sample in the hyperchannel valve gating reagent bottle or the sample in the automatic sampler, pump carries out flow velocity and flow control to sample.
Connected system is a hyperchannel valve, and the flow channel of hyperchannel valve is respectively sample channel, eluent channel and two and washes post liquid passage, and the flow pass of hyperchannel valve is connected to chromatographic column.Connected system select to be communicated with sample or elutriant or to wash post liquid, and corresponding solution is input to correspondingly separates in the chromatographic column or clean.
Chromatographic column is connected to a hyperchannel valve, and the first-class road of this hyperchannel valve connects the waste liquid cylinder, and a stream connection detector, detector are connected to a hyperchannel valve, and this hyperchannel valve connects waste liquid cylinder and automatic collector.The hyperchannel valve is controlled by Controlling System, solution after chromatographic column cleaned does not need just to detect and directly enters in the waste liquid cylinder, through isolating sample after testing device detect, will collect in the needed component input automatic collector, impurity etc. is entered the waste liquid cylinder.Automatic collector can be collected different components respectively in the different spaces according to detected component.
A kind of liquid phase separation method of high-throughput polypeptide, its step comprises: (a) dosing, the preparation elutriant, elutriant is pressed required mixed by different eluents; (b) sample introduction is selected required isolating sample, and sample is imported connected system; (c) separate, in the chromatographic column sample is separated; (d) clean, it is cleaned with washing post solution; (e) detect, the sample after separating is carried out online detection, judge whether to reach the parameter of setting; (f) collect, the sample that meets setup parameter is collected, waste liquid is entered the waste liquid cylinder; (g) control, the orderly function automatically as required of control total system.
Step (a) dosing, comprise by pump the flow velocity and the flow of eluent are controlled, thereby control the ratio of different eluents, the elutriant after the preparation detects through pressure detector, whether the pressure of judging elutriant meets the requirements, and judges whether to need to adjust pump with this.
Step (f) is collected, also comprise with during the chromatographic column running clearance to its wash liquid directly enter the waste liquid cylinder, to flow into detector through the liquid after separating, detector will meet the sample inflow automatic collector of setup parameter to be collected, and the sample that does not meet setup parameter enters the waste liquid cylinder.
Step (g) control comprises: come controlled liq to flow through the flow velocity and the flow of pump by control pump; By control hyperchannel valve the passage of required connection is carried out conducting; Also control automatic sampler and automatic collector,, will separate the different spaces of the different sample collection in back to automatic collector with needed sample feeding.
The beneficial effect that the present invention brings is: the present invention adopts hyperchannel to work simultaneously sample is separated, in total system, all adopt Controlling System to control to dosing, sample introduction, separation, cleaning and collection, make total system to circulate and carry out work in an orderly manner, realize full-automatic, velocity of separation of the present invention can satisfy the separation of high-throughout synthetic peptide than piece.The present invention also adopts automatic sampler and automatic collector, can store several samples in the automatic sampler, automatic collector can to sample discern with and be collected in different spaces.
Description of drawings
Accompanying drawing 1 is a kind of structural representation of the present invention;
1-automatic sampler, 2-elutriant, 3-balance liquid, 4-sample, 5-hyperchannel valve one, 6-sample introduction pipe, 7-sampling system, 8-pump, 9-chromatography column, 10-sample collection device, 11-control workstation, 12-UV-detector, 13-LC/MS, 14-HPLC/MS, 15-infrared spectra or Raman spectrum, 16-hyperchannel valve two
Sampling system comprises two sample intake passages, sample reagent bottle (4), equilibrium liquid (3) and the elution reagent (2) that the passage connection capacity is bigger, a passage connects automatic sampler (1), automatic sampler (1) can separately store the littler sample of multiple capacity, two sample intake passages are connected to multiple-way valve one (5) jointly, multiple-way valve one (5) is connected to pump (8) by sampling device (7), and pump (8) is connected to connected system; Multiple detector comprises UV-detector (12), LC/MS (13), HPLC/MS (14), infrared spectrum or Raman spectrum (15).
Embodiment:
Below by embodiment, and in conjunction with the accompanying drawings, technical scheme of the present invention is described in further detail.
Embodiment 1: high-efficient integrated system purifying 21 peptide compounds in enormous quantities
A kind of high-efficient integrated system for separation and purification, comprise liquid mixing system, sampling system, separation system, cleaning system, connected system, detector and gathering system, wherein liquid mixing system, sampling system and cleaning system are connected to connected system, and connected system is connected to separation system.
In 30% acetonitrile solution 5L, supersound process placed sample bottle (4) with consoluet sample more than 10 minutes with synthetic 21 peptide dissolving crude products; Be ready to balanced solution (water+1%TFA solution) 5L, in balance liquid bottle (3); Be ready to elutriant (acetonitrile+1%TFA solution) 5L, place elutriant bottle (2).And choose suitable chromatography column (9) C4 reversed-phase column, and correctly be connected in the system.
Balance pillar: set program: comprise sample introduction order, sample size and sample introduction by workstation (11).Under the control of hyperchannel valve one (5), be communicated with balance liquid (3) to sampling system (7) by pump (8); Balance liquid enters chromatography column (9) under the effect of pump, the effusive solution of chromatography column is connected to UV-detector (12) through hyperchannel, and whether balance is good to judge chromatography column by the baseline of UV-detector.From the effusive sample of UV-detector directly in the waste liquid bottle to the sample collection device (10).
Sample introduction: by time variable control, after pillar balance to baseline has been put down, switch to sample solution (4), be communicated with sample solution (4) to sampling system (7) by pump (8) by hyperchannel valve one (5); Balance liquid enters chromatography column (9) under the effect of pump, the effusive solution of chromatography column is connected to UV-detector (12), LC/MS (13), infrared spectra or Raman spectrum (15) through hyperchannel, result by online detection judges target product, and whether stream wears out, and,, collects target product stream sample if wearing to sample collection device (10) according to detected result.
Wash-out: after last sample program is finished, by hyperchannel valve one (5), switch to elutriant (2), under the effect of elutriant, target product and magazine are eluted respectively, and the product that elutes is flowed through hyperchannel at thread detector, by online detected result, determine target product, by sample collection device collect sample respectively, be divided into salable product, unacceptable product and waste liquid.Salable product can flow directly into next step production, unacceptable product upper prop repurity again, and waste liquid can directly be thrown away.
Post cleans: after having collected target product, can directly clean with the elutriant coupled columns, guarantee the regeneration of pillar.
By this method, can realize that the automatization of extensive separation and purification is carried out, improved efficient greatly, reduced the waste of reagent.
Embodiment 2: high-efficient integrated system purifying 32 peptide compounds (small amount of sample)
A kind of high-efficient integrated system for separation and purification, comprise liquid mixing system, sampling system, separation system, cleaning system, connected system, detector and gathering system, wherein liquid mixing system, sampling system and cleaning system are connected to connected system, and connected system is connected to separation system.
In 30% acetonitrile solution 100ml, supersound process placed sample bottle (4) with consoluet sample more than 5 minutes with synthetic 32 peptide dissolving crude products; Be ready to balanced solution (water+1%TFA solution) 1L, in balance liquid bottle (3); Be ready to elutriant (acetonitrile+1%TFA solution) 1L, place elutriant bottle (2).And choose suitable chromatography column (9) C4 reversed-phase column, and correctly be connected in the system.
Balance pillar: set program: comprise sample introduction order, sample size and sample introduction by workstation (11).Under the control of hyperchannel valve one (5), be communicated with balance liquid (3) to sampling system (7) by pump (8); Balance liquid enters chromatography column (9) under the effect of pump, the effusive solution of chromatography column is connected to UV-detector (12) through hyperchannel, and whether balance is good to judge chromatography column by the baseline of UV-detector.From the effusive sample of UV-detector directly in the waste liquid bottle to the sample collection device (10).
Sample introduction: by time variable control, after pillar balance to baseline has been put down, switch to automatic sampler (1), be communicated with sample in the automatic sampler (1) to sampling system (7) by pump (8) by hyperchannel valve one (5); Balance liquid enters chromatography column (9) under the effect of pump, the effusive solution of chromatography column is connected to UV-detector (12), LC/MS (13), infrared spectra or Raman spectrum (15) through hyperchannel, result by online detection judges target product, and whether stream wears out, and,, collects target product stream sample if wearing to sample collection device (10) according to detected result.。
Wash-out: after last sample program is finished, by hyperchannel valve one (5), switch to elutriant (2), under the effect of elutriant, target product and magazine are eluted respectively, and the product that elutes is flowed through hyperchannel at thread detector, by online detected result, determine target product, by sample collection device collect sample respectively, be divided into salable product, unacceptable product and waste liquid.Salable product can flow directly into next step production, unacceptable product upper prop repurity again, and waste liquid can directly be thrown away.
Post cleans: after having collected target product, can directly clean with the elutriant coupled columns, guarantee the regeneration of pillar.
By this method purifying, can improve the flux of peptide purification greatly.
Claims (10)
1. high-efficient integrated system for separation and purification, comprise liquid mixing system, sampling system, separation system, cleaning system, connected system, detector, gathering system and control corresponding system, it is characterized in that described separation system is made of chromatographic column, liquid mixing system, sampling system and cleaning system are connected to connected system, connected system is connected to separation system, and separation system is connected to various detectors and gathering system by hyperchannel.
2. high-efficient integrated system for separation and purification according to claim 1 is characterized in that described liquid mixing system comprises some kinds of eluents, correspondingly is provided with pump on the stream of each eluent, and pump is connected to mixing tank jointly, and mixing tank is connected to connected system.
3. high-efficient integrated system for separation and purification according to claim 1, it is characterized in that described sampling system comprises two sample intake passages, sample reagent bottle (4), balance liquid (3) and the elution reagent (2) that the passage connection capacity is bigger, a passage connects automatic sampler (1), automatic sampler (1) can separately store the less sample of multiple capacity, two sample intake passages are connected to hyperchannel valve one (5) jointly, hyperchannel valve one (5) is connected to pump (8) by sampling device (7), and pump (8) is connected to connected system.
4. according to claim 1 or 2 or 3 described high-efficient integrated system for separation and purification, it is characterized in that described connected system is a hyperchannel valve, the flow channel of hyperchannel valve is respectively sample channel, eluent channel and balance liquid passage and automatic sampling passage.
5. high-efficient integrated system for separation and purification according to claim 1 is characterized in that described hyperchannel coupled unit, is directly connected on the multiple detector, realizes the online detection of hyperchannel.
6. high-efficient integrated system for separation and purification according to claim 5 is characterized in that described multiple detector comprises UV-detector (12), LC/MS (13), HPLC/MS (14), infrared spectra or Raman spectrum (15).
7. high-efficient integrated system for separation and purification separation method, its step comprises:
(a) dosing, the preparation elutriant, elutriant is pressed required mixed by different eluents;
(b) sample introduction is selected required isolating sample, and sample is imported connected system;
(c) be communicated with, chromatographic column is selected to be communicated with sample or elutriant respectively or washed post liquid;
(d) separate, in chromatographic column, sample is separated;
(e) detect, the sample after separating is carried out determining target product by online detection;
(f) collect, the sample that meets setup parameter is collected, waste liquid is entered the waste liquid cylinder;
(g) control, the orderly function automatically as required of control total system.Clean, after sample separation finishes, it is cleaned with washing post solution.
8. the separation method of high-efficient integrated system for separation and purification according to claim 7 is characterized in that described step (a) dosing, comprises by pump the flow velocity and the flow of eluent are controlled, thereby control the ratio of different eluents.
9. high-efficient integrated system for separation and purification separation method according to claim 7, it is characterized in that described step (g) collection, also comprise with during the chromatographic column running clearance to its wash liquid directly enter the waste liquid cylinder, to flow into detector through the liquid after separating, detector will meet the sample inflow automatic collector of component requirement to be collected, and the sample that does not meet the component requirement enters the waste liquid cylinder.
10. high-efficient integrated system for separation and purification separation method according to claim 7 is characterized in that described step (g) control comprises: come controlled liq to flow through the flow velocity and the flow of pump by control pump; By control hyperchannel valve the passage of required connection is carried out conducting; Also control automatic sampler and automatic collector,, will separate the different spaces of the different sample collection in back to automatic collector with needed sample feeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810162337A CN101747407A (en) | 2008-12-02 | 2008-12-02 | High-efficient integrated system for separation and purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810162337A CN101747407A (en) | 2008-12-02 | 2008-12-02 | High-efficient integrated system for separation and purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101747407A true CN101747407A (en) | 2010-06-23 |
Family
ID=42475079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810162337A Pending CN101747407A (en) | 2008-12-02 | 2008-12-02 | High-efficient integrated system for separation and purification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101747407A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103201003A (en) * | 2010-09-20 | 2013-07-10 | Abbvie公司 | Purification of antibodies using simulated moving bed chromatography |
| CN108152243A (en) * | 2017-12-12 | 2018-06-12 | 福建省测试技术研究所（福建省分析测试中心） | A kind of compound system nonionic surfactant qualitative determination method |
| CN112630332A (en) * | 2020-12-14 | 2021-04-09 | 安徽标王农牧有限公司 | Embryo egg separation and purification system based on liquid chromatogram |
| CN114047274A (en) * | 2021-09-22 | 2022-02-15 | 四川轻化工大学 | Separation and extraction system for nuclides in radioactive sample |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2348203Y (en) * | 1998-07-10 | 1999-11-10 | 崔宝科 | Multiple-way valve |
| CN1289922A (en) * | 2000-11-21 | 2001-04-04 | 中国人民解放军军事医学科学院放射医学研究所 | Biochemical Process for preparing liquid-phase chromatograph |
| CN1301964A (en) * | 1999-12-28 | 2001-07-04 | 福州大学 | Intelligent integrated automatic liquid phase chromatographic system |
| CN1319736A (en) * | 2000-03-24 | 2001-10-31 | 株式会社百尼尔 | Device for multi-channel quantitative controlled valve |
| CN2476022Y (en) * | 2001-04-20 | 2002-02-06 | 阎超 | Multichannel pressure capillary tube electrochromatographic biological sample analyser |
| CN1749750A (en) * | 2004-09-13 | 2006-03-22 | 聂智明 | High efficiency liquid phase chromatograph |
| CN101169393A (en) * | 2006-10-25 | 2008-04-30 | 中国科学院大连化学物理研究所 | Two-dimensional array liquid phase chromatogram-mass spectrometry combination method |
-
2008
- 2008-12-02 CN CN200810162337A patent/CN101747407A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2348203Y (en) * | 1998-07-10 | 1999-11-10 | 崔宝科 | Multiple-way valve |
| CN1301964A (en) * | 1999-12-28 | 2001-07-04 | 福州大学 | Intelligent integrated automatic liquid phase chromatographic system |
| CN1319736A (en) * | 2000-03-24 | 2001-10-31 | 株式会社百尼尔 | Device for multi-channel quantitative controlled valve |
| CN1289922A (en) * | 2000-11-21 | 2001-04-04 | 中国人民解放军军事医学科学院放射医学研究所 | Biochemical Process for preparing liquid-phase chromatograph |
| CN2476022Y (en) * | 2001-04-20 | 2002-02-06 | 阎超 | Multichannel pressure capillary tube electrochromatographic biological sample analyser |
| CN1749750A (en) * | 2004-09-13 | 2006-03-22 | 聂智明 | High efficiency liquid phase chromatograph |
| CN101169393A (en) * | 2006-10-25 | 2008-04-30 | 中国科学院大连化学物理研究所 | Two-dimensional array liquid phase chromatogram-mass spectrometry combination method |
Non-Patent Citations (1)
| Title |
|---|
| 房其年等: "ZB-2型制备液相色谱仪的研制", 《色谱》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103201003A (en) * | 2010-09-20 | 2013-07-10 | Abbvie公司 | Purification of antibodies using simulated moving bed chromatography |
| CN103201003B (en) * | 2010-09-20 | 2016-04-13 | Abbvie公司 | Adopt SMBC antibody purification |
| CN105753933A (en) * | 2010-09-20 | 2016-07-13 | Abbvie 公司 | Purification Of Antibodies By Using Simulated Moving Bed Chromatography |
| TWI617571B (en) * | 2010-09-20 | 2018-03-11 | 艾伯維有限公司 | Purification of antibodies using simulated moving bed chromatography |
| CN108152243A (en) * | 2017-12-12 | 2018-06-12 | 福建省测试技术研究所（福建省分析测试中心） | A kind of compound system nonionic surfactant qualitative determination method |
| CN108152243B (en) * | 2017-12-12 | 2020-06-30 | 福建省测试技术研究所（福建省分析测试中心） | Qualitative determination method for nonionic surfactant in compound system |
| CN112630332A (en) * | 2020-12-14 | 2021-04-09 | 安徽标王农牧有限公司 | Embryo egg separation and purification system based on liquid chromatogram |
| CN114047274A (en) * | 2021-09-22 | 2022-02-15 | 四川轻化工大学 | Separation and extraction system for nuclides in radioactive sample |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6344172B1 (en) | Protein chromatography system | |
| US8268625B2 (en) | Method of measuring glycated hemoglobin concentration and concentration measuring apparatus | |
| CN101782587B (en) | Instrument system suitable for synchronous on-line detection of adsorption spectrum and fluorescence spectrum | |
| CN102166433B (en) | Multichannel parallel automatic solid phase extraction device | |
| CN103698428B (en) | Vehicle-mounted portable liquid chromatographic instrument | |
| US20180340917A1 (en) | Chromatography system with guard columns | |
| CN102967681A (en) | Multi-dimensional ion chromatographic analysis system | |
| CN101747407A (en) | High-efficient integrated system for separation and purification | |
| CN107106930A (en) | Method and apparatus for performing liquid chromatography purification | |
| CN1749750A (en) | High efficiency liquid phase chromatograph | |
| CN111318052A (en) | Laboratory type continuous flow chromatography system | |
| US8021887B2 (en) | Method of measuring glycated hemoglobin concentration | |
| CN110923366A (en) | Method for on-line monitoring yield and quality of target protein | |
| CN103293253B (en) | Efficient purification-analysis system for biotechnological medicine and medicine separation detection method thereof | |
| EP0359322A2 (en) | Liquid chromatography | |
| CN212375283U (en) | System for on-line monitoring yield and quality of target protein | |
| CN209215064U (en) | A kind of separation of close and distant water component and collection device based on on-line checking | |
| CN111643928B (en) | Automatic solid phase extraction device | |
| CN208171864U (en) | Semiautomatic plant and detection device based on Solid Phase Extraction | |
| JP5087727B2 (en) | Apparatus and method for measuring molecular weight distribution of organic polymer component | |
| CN110455942A (en) | A kind of online Ultra Performance Liquid Chromatography instrument of three-dimensional | |
| CN110286173A (en) | Liquid-chromatography apparatus is used in a kind of detection of blood concentration | |
| CN2762132Y (en) | High-efficient liquid phase chromatograph | |
| CN109557199A (en) | A kind of two-dimensional liquid chromatography full-automatic valve control system and method | |
| CN115406995B (en) | Device, method and application for nuclear fuel burnup analysis and fission product preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20100623 |
|
| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 310018, No. 12, No. 69, technical development zone, economic city, Zhejiang, Hangzhou Applicant after: CHINESE PEPTIDE COMPANY Address before: 310018, No. 12, No. 69, technical development zone, economic city, Zhejiang, Hangzhou Applicant before: Hangzhou Chinese Peptide Co., Ltd. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: ZHONGTAI BIO-CHEM. CO., LTD., HANGZHOU TO: CHINESE PEPTIDE CO., LTD. |